95 results on '"Alberini CM"'
Search Results
2. C/EBP transcription factors expressed in the mammalian brain
- Author
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Ingrassia, R, primary, Gulotta, T, additional, Pollonini, G, additional, Ungari, M, additional, Facchetti, F, additional, Albertini, A, additional, and Alberini, CM, additional
- Published
- 1996
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3. Insulin-like growth factor 2 (IGF-2) rescues social deficits in NLG3 -/ y mouse model of ASDs.
- Author
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Pizzarelli R, Pimpinella D, Jacobs C, Tartacca A, Kullolli U, Monyer H, Alberini CM, and Griguoli M
- Abstract
Autism spectrum disorders (ASDs) comprise developmental disabilities characterized by impairments of social interaction and repetitive behavior, often associated with cognitive deficits. There is no current treatment that can ameliorate most of the ASDs symptomatology; thus, identifying novel therapies is urgently needed. Here, we used the Neuroligin 3 knockout mouse (NLG3
-/ y ), a model that recapitulates the social deficits reported in ASDs patients, to test the effects of systemic administration of IGF-2, a polypeptide that crosses the blood-brain barrier and acts as a cognitive enhancer. We show that systemic IGF-2 treatment reverses the typical defects in social interaction and social novelty discrimination reflective of ASDs-like phenotypes. This effect was not accompanied by any change in spontaneous glutamatergic synaptic transmission in CA2 hippocampal region, a mechanism found to be crucial for social novelty discrimination. However, in both NLG3+/ y and NLG3-/ y mice IGF-2 increased cell excitability. Although further investigation is needed to clarify the cellular and molecular mechanisms underpinning IGF-2 effect on social behavior, our findings highlight IGF-2 as a potential pharmacological tool for the treatment of social dysfunctions associated with ASDs., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Pizzarelli, Pimpinella, Jacobs, Tartacca, Kullolli, Monyer, Alberini and Griguoli.)- Published
- 2024
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4. Neuronal activity drives IGF2 expression from pericytes to form long-term memory.
- Author
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Pandey K, Bessières B, Sheng SL, Taranda J, Osten P, Sandovici I, Constancia M, and Alberini CM
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- Animals, Mice, Rats, Hippocampus metabolism, Memory, Long-Term, Signal Transduction, Neurons metabolism, Pericytes
- Abstract
Investigations of memory mechanisms have been, thus far, neuron centric, despite the brain comprising diverse cell types. Using rats and mice, we assessed the cell-type-specific contribution of hippocampal insulin-like growth factor 2 (IGF2), a polypeptide regulated by learning and required for long-term memory formation. The highest level of hippocampal IGF2 was detected in pericytes, the multi-functional mural cells of the microvessels that regulate blood flow, vessel formation, the blood-brain barrier, and immune cell entry into the central nervous system. Learning significantly increased pericytic Igf2 expression in the hippocampus, particularly in the highly vascularized stratum lacunosum moleculare and stratum moleculare layers of the dentate gyrus. Igf2 increases required neuronal activity. Regulated hippocampal Igf2 knockout in pericytes, but not in fibroblasts or neurons, impaired long-term memories and blunted the learning-dependent increase of neuronal immediate early genes (IEGs). Thus, neuronal activity-driven signaling from pericytes to neurons via IGF2 is essential for long-term memory., Competing Interests: Declaration of interests C.M.A. is an inventor of patents filed by NYU entitled on the use of IGF2 receptor agonist ligands for treatment of neurodevelopmental disorders and neurodegenerative diseases. C.M.A. is a founder of a company seeking to develop compounds covered by the patents., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
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5. Excessive Protein Accumulation and Impaired Autophagy in the Hippocampus of Angelman Syndrome Modeled in Mice.
- Author
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Aria F, Pandey K, and Alberini CM
- Subjects
- Mice, Animals, Hippocampus metabolism, Brain metabolism, Learning, Autophagy, Angelman Syndrome
- Abstract
Background: Angelman syndrome (AS), a neurodevelopmental disorder caused by abnormalities of the 15q11.2-q13.1 chromosome region, is characterized by impairment of cognitive and motor functions, sleep problems, and seizures. How the genetic defects of AS produce these neurological symptoms is unclear. Mice modeling AS (AS mice) accumulate activity-regulated cytoskeleton-associated protein (ARC/ARG3.1), a neuronal immediate early gene (IEG) critical for synaptic plasticity. This accumulation suggests an altered protein metabolism., Methods: Focusing on the dorsal hippocampus (dHC), a brain region critical for memory formation and cognitive functions, we assessed levels and tissue distribution of IEGs, de novo protein synthesis, and markers of protein synthesis, endosomes, autophagy, and synaptic functions in AS mice at baseline and following learning. We also tested autophagic flux and memory retention following autophagy-promoting treatment., Results: AS dHC exhibited accumulation of IEGs ARC, FOS, and EGR1; autophagy proteins MLP3B, SQSTM1, and LAMP1; and reduction of the endosomal protein RAB5A. AS dHC also had increased levels of de novo protein synthesis, impaired autophagic flux with accumulation of autophagosome, and altered synaptic protein levels. Contextual fear conditioning significantly increased levels of IEGs and autophagy proteins, de novo protein synthesis, and autophagic flux in the dHC of normal mice, but not in AS mice. Enhancing autophagy in the dHC alleviated AS-related memory and autophagic flux impairments., Conclusions: A major biological deficit of AS brain is a defective protein metabolism, particularly that dynamically regulated by learning, resulting in stalled autophagy and accumulation of neuronal proteins. Activating autophagy ameliorates AS cognitive impairments and dHC protein accumulation., (Copyright © 2023 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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6. IGF2 in memory, neurodevelopmental disorders, and neurodegenerative diseases.
- Author
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Alberini CM
- Subjects
- Animals, Brain metabolism, Humans, Alzheimer Disease, Neurodegenerative Diseases metabolism, Neurodevelopmental Disorders, Parkinson Disease
- Abstract
Insulin-like growth factor 2 (IGF2) emerged as a critical mechanism of synaptic plasticity and learning and memory. Deficits in IGF2 in the brain, serum, or cerebrospinal fluid (CSF) are associated with brain diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and amyotrophic lateral sclerosis (ALS). Increasing IGF2 levels enhances memory in healthy animals and reverses numerous symptoms in laboratory models of aging, neurodevelopmental disorders, and neurodegenerative diseases. These effects occur via the IGF2 receptor (IGF2R) - a receptor that is highly expressed in neurons and regulates protein trafficking, synthesis, and degradation. Here, I summarize the current knowledge regarding IGF2 expression and functions in the brain, particularly in memory, and propose a novel conceptual model for IGF2/IGF2R mechanisms of action in brain health and diseases., Competing Interests: Declaration of interests C.M.A. is the founder and current president of Ritrova Therapeutics, Inc., a start-up that stemmed from IGF2 and IGF2R studies and aims to develop treatment for neurodevelopmental disorders and neurodegenerative diseases., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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7. Hippocampal parvalbumin interneurons play a critical role in memory development.
- Author
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Miranda JM, Cruz E, Bessières B, and Alberini CM
- Subjects
- Child, Preschool, Rats, Humans, Animals, Interneurons physiology, Hippocampus physiology, Parvalbumins, Memory, Episodic
- Abstract
Episodic memories formed in early childhood rapidly decay, but their latent traces remain stored long term. These memories require the dorsal hippocampus (dHPC) and seem to undergo a developmental critical period. It remains to be determined whether the maturation of parvalbumin interneurons (PVIs), a major mechanism of critical periods, contributes to memory development. Here, we show that episodic infantile learning significantly increases the levels of parvalbumin in the dHPC 48 h after training. Chemogenetic inhibition of PVIs before learning indicated that these neurons are required for infantile memory formation. A bilateral dHPC injection of the γ-aminobutyric acid type A receptor agonist diazepam after training elicited long-term memory expression in infant rats, although direct PVI chemogenetic activation had no effect. Finally, PVI activity was required for brain-derived neurotrophic factor (BDNF)-dependent maturation of memory competence, i.e., adult-like long-term memory expression. Thus, dHPC PVIs are critical for the formation of infantile memories and for memory development., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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8. Differential role of neuronal glucose and PFKFB3 in memory formation during development.
- Author
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Cruz E, Bessières B, Magistretti P, and Alberini CM
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- Animals, Astrocytes metabolism, Child, Glucose Transporter Type 3 genetics, Glucose Transporter Type 3 metabolism, Humans, Neurons metabolism, Phosphofructokinase-2 genetics, Rats, Glucose metabolism, Glycolysis, Phosphofructokinase-2 metabolism
- Abstract
The consumption of glucose in the brain peaks during late childhood; yet, whether and how glucose metabolism is differentially regulated in the brain during childhood compared to adulthood remains to be understood. In particular, it remains to be determined how glucose metabolism is involved in behavioral activations such as learning. Here we show that, compared to adult, the juvenile rat hippocampus has significantly higher mRNA levels of several glucose metabolism enzymes belonging to all glucose metabolism pathways, as well as higher levels of the monocarboxylate transporters MCT1 and MCT4 and the glucose transporters endothelial-GLUT1 and GLUT3 proteins. Furthermore, relative to adults, long-term episodic memory formation in juvenile animals requires significantly higher rates of aerobic glycolysis and astrocytic-neuronal lactate coupling in the hippocampus. Only juvenile but not adult long-term memory formation recruits GLUT3, neuronal 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) and more efficiently engages glucose in the hippocampus. Hence, compared to adult, the juvenile hippocampus distinctively regulates glucose metabolism pathways, and formation of long-term memory in juveniles involves differential neuronal glucose metabolism mechanisms., (© 2022 Wiley Periodicals LLC.)
- Published
- 2022
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9. Computational analysis of memory consolidation following inhibitory avoidance (IA) training in adult and infant rats: Critical roles of CaMKIIα and MeCP2.
- Author
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Zhang Y, Smolen P, Alberini CM, Baxter DA, and Byrne JH
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- Animals, Avoidance Learning physiology, Hippocampus physiology, Humans, Memory, Long-Term physiology, Methyl-CpG-Binding Protein 2 genetics, Methyl-CpG-Binding Protein 2 metabolism, Methyl-CpG-Binding Protein 2 pharmacology, Rats, Brain-Derived Neurotrophic Factor metabolism, Memory Consolidation
- Abstract
Key features of long-term memory (LTM), such as its stability and persistence, are acquired during processes collectively referred to as consolidation. The dynamics of biological changes during consolidation are complex. In adult rodents, consolidation exhibits distinct periods during which the engram is more or less resistant to disruption. Moreover, the ability to consolidate memories differs during developmental periods. Although the molecular mechanisms underlying consolidation are poorly understood, the initial stages rely on interacting signaling pathways that regulate gene expression, including brain-derived neurotrophic factor (BDNF) and Ca2+/calmodulin-dependent protein kinase II α (CaMKIIα) dependent feedback loops. We investigated the ways in which these pathways may contribute to developmental and dynamical features of consolidation. A computational model of molecular processes underlying consolidation following inhibitory avoidance (IA) training in rats was developed. Differential equations described the actions of CaMKIIα, multiple feedback loops regulating BDNF expression, and several transcription factors including methyl-CpG binding protein 2 (MeCP2), histone deacetylase 2 (HDAC2), and SIN3 transcription regulator family member A (Sin3a). This model provides novel explanations for the (apparent) rapid forgetting of infantile memory and the temporal progression of memory consolidation in adults. Simulations predict that dual effects of MeCP2 on the expression of bdnf, and interaction between MeCP2 and CaMKIIα, play critical roles in the rapid forgetting of infantile memory and the progress of memory resistance to disruptions. These insights suggest new potential targets of therapy for memory impairment., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2022
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10. Introduction to the special issue on the ontogeny of hippocampal functions.
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Ohana O, Alberini CM, and Donato F
- Subjects
- Hippocampus, Neurons
- Published
- 2022
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11. Metabolomic profiling reveals a differential role for hippocampal glutathione reductase in infantile memory formation.
- Author
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Bessières B, Cruz E, and Alberini CM
- Subjects
- Animals, Female, Male, Metabolome, Rats, Rats, Long-Evans, Glutathione Reductase metabolism, Hippocampus metabolism, Memory, Episodic, Memory, Long-Term physiology
- Abstract
The metabolic mechanisms underlying the formation of early-life episodic memories remain poorly characterized. Here, we assessed the metabolomic profile of the rat hippocampus at different developmental ages both at baseline and following episodic learning. We report that the hippocampal metabolome significantly changes over developmental ages and that learning regulates differential arrays of metabolites according to age. The infant hippocampus had the largest number of significant changes following learning, with downregulation of 54 metabolites. Of those, a large proportion was associated with the glutathione-mediated cellular defenses against oxidative stress. Further biochemical, molecular, and behavioral assessments revealed that infantile learning evokes a rapid and persistent increase in the activity of neuronal glutathione reductase, the enzyme that regenerates reduced glutathione from its oxidized form. Inhibition of glutathione reductase selectively impaired long-term memory formation in infant but not in juvenile and adult rats, confirming its age-specific role. Thus, metabolomic profiling revealed that the hippocampal glutathione-mediated antioxidant pathway is differentially required for the formation of infantile memory., Competing Interests: BB, EC, CA No competing interests declared, (© 2021, Bessières et al.)
- Published
- 2021
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12. Associative memory persistence in 3- to 5-year-olds.
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Saragosa-Harris NM, Cohen AO, Shen X, Sardar H, Alberini CM, and Hartley CA
- Subjects
- Amnesia, Child, Preschool, Hippocampus, Humans, Learning, Mental Recall, Memory, Episodic
- Abstract
Adults struggle to recollect episodic memories from early life. This phenomenon-referred to as "infantile" and "childhood amnesia"-has been widely observed across species and is characterized by rapid forgetting from birth until early childhood. While a number of studies have focused on infancy, few studies have examined the persistence of memory for newly learned associations during the putative period of childhood amnesia. In this study, we investigated forgetting in 137 children ages 3-5 years old by using an interactive storybook task. We assessed associative memory between subjects after 5-min, 24-h, and 1-week delay periods. Across all delays, we observed a significant increase in memory performance with age. While all ages demonstrated above-chance memory performance after 5-min and 24-h delays, we observed chance-level memory accuracy in 3-year-olds following a 1-week delay. The observed age differences in associative memory support the proposal that hippocampal-dependent memory systems undergo rapid development during the preschool years. These data have the potential to inform future work translating memory persistence and malleability research from rodent models to humans by establishing timescales at which we expect young children to forget newly learned associations., (© 2021 John Wiley & Sons Ltd.)
- Published
- 2021
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13. Recovery of memory from infantile amnesia is developmentally constrained.
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Bisaz R, Bessières B, Miranda JM, Travaglia A, and Alberini CM
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- Animals, Brain, Conditioning, Operant, Mental Recall, Mice, Rats, Amnesia, Memory, Episodic
- Abstract
Episodic memories formed during infancy are rapidly forgotten, a phenomenon associated with infantile amnesia, the inability of adults to recall early-life memories. In both rats and mice, infantile memories, although not expressed, are actually stored long term in a latent form. These latent memories can be reinstated later in life by certain behavioral reminders or by artificial reactivations of neuronal ensembles activated at training. Whether the recovery of infantile memories is limited by developmental age, maternal presence, or contingency of stimuli presentation remains to be determined. Here, we show that the return of inhibitory avoidance memory in rats following a behavioral reactivation consisting of an exposure to the context (conditioned stimuli [CS]) and footshock (unconditioned stimuli [US]) given in a temporally unpaired fashion, is evident immediately after US and is limited by the developmental age at which the reactivations are presented; however, it is not influenced by maternal presence or the time interval between training and reactivation. We conclude that one limiting factor for infantile memory reinstatement is developmental age, suggesting that a brain maturation process is necessary to allow the recovery of a "lost" infantile memory., (© 2021 Bisaz et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2021
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14. Editorial.
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Alberini CM
- Published
- 2021
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15. Autophagy coupled to translation is required for long-term memory.
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Pandey K, Yu XW, Steinmetz A, and Alberini CM
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- Animals, Avoidance Learning physiology, Beclin-1 metabolism, Fluorescent Antibody Technique, Gene Knockdown Techniques, Hippocampus metabolism, Hippocampus physiology, Learning physiology, Lysosomal Membrane Proteins metabolism, Male, Microtubule-Associated Proteins metabolism, Rats, Rats, Long-Evans, Sequestosome-1 Protein metabolism, Autophagy physiology, Memory, Long-Term physiology, Protein Biosynthesis physiology
- Abstract
An increase in protein synthesis following learning is a fundamental and evolutionarily conserved mechanism of long-term memory. To maintain homeostasis, this protein synthesis must be counterbalanced by mechanisms such as protein degradation. Recent studies reported that macroautophagy/autophagy, a major protein degradation mechanism, is required for long-term memory formation. However, how learning regulates autophagy and recruits it into long-term memory formation remains to be established. Here, we show that inhibitory avoidance in rats significantly increases the levels of autophagy and lysosomal degradation proteins, including BECN1/beclin 1, LC3-II, SQSTM1/p62 and LAMP1, as well as autophagic flux in the hippocampus. Moreover, pharmacological inhibition or targeted molecular disruption of the learning-induced autophagy impairs long-term memory, leaving short-term memory intact. The increase in autophagy proteins results from active translation of their mRNA and not from changes in their total mRNA levels. Additionally, the induction of autophagy requires the immediate early gene Arc / Arg3.1 . Finally, in contrast to classical regulation of autophagy in other systems, we found that the increase in autophagy upon learning is dispensable for the increase in protein synthesis. We conclude that coupling between learning-induced translation and autophagy, rather than translation per se , is an essential mechanism of long-term memory. Abbreviations: AAV: adeno-associated virus; ARC/ARG3.1: activity regulated cytoskeletal-associated protein; ATG: autophagy related; DG: dentate gyrus; GFP: green fluorescent protein; IA: inhibitory avoidance; LAMP1: lysosomal-associated membrane protein 1; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; ODN: oligodeoxynucleotide; qPCR: quantitative polymerase chain reaction; SBI: SBI0206965; SQSTM1/p62: sequestosome 1; SUnSET: surface sensing of translation; TRAP: translating ribosome affinity purification; ULK1: unc-51 like kinase 1.
- Published
- 2021
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16. Distinct Transcriptomic Profiles in the Dorsal Hippocampus and Prelimbic Cortex Are Transiently Regulated following Episodic Learning.
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Katzman A, Khodadadi-Jamayran A, Kapeller-Libermann D, Ye X, Tsirigos A, Heguy A, and Alberini CM
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- Animals, Fear physiology, Fear psychology, Male, Rats, Rats, Long-Evans, Avoidance Learning physiology, Gene Regulatory Networks physiology, Hippocampus physiology, Memory, Episodic, Prefrontal Cortex physiology, Transcriptome physiology
- Abstract
A fundamental, evolutionarily conserved biological mechanism required for long-term memory formation is rapid induction of gene transcription upon learning in relevant brain areas. For episodic types of memories, two regions undergoing this transcription are the dorsal hippocampus (dHC) and prelimbic (PL) cortex. Whether and to what extent these regions regulate similar or distinct transcriptomic profiles upon learning remain to be understood. Here, we used RNA sequencing in the dHC and PL cortex of male rats to profile their transcriptomes in untrained conditions (baseline) and at 1 h and 6 d after inhibitory avoidance learning. We found that, of 33,713 transcripts, >14,000 were significantly expressed at baseline in both regions and ∼3000 were selectively enriched in each region. Gene Ontology biological pathway analyses indicated that commonly expressed pathways included synapse organization, regulation of membrane potential, and vesicle localization. The enriched pathways in the dHC were gliogenesis, axon development, and lipid modification, while in the PL cortex included vesicle localization and synaptic vesicle cycle. At 1 h after learning, 135 transcripts changed significantly in the dHC and 478 in the PL cortex; of these, only 34 were shared. Biological pathways most significantly regulated by learning in the dHC were protein dephosphorylation, glycogen and glucan metabolism, while in the PL cortex were axon development and axonogenesis. The transcriptome profiles returned to baseline by 6 d after training. Thus, a significant portion of dHC and PL cortex transcriptomic profiles is divergent, and their regulation upon learning is largely distinct and transient. SIGNIFICANCE STATEMENT Long-term episodic memory formation requires gene transcription in several brain regions, including the hippocampus and PFC. The comprehensive profiles of the dynamic mRNA changes that occur in these regions following learning are not well understood. Here, we performed RNA sequencing in the dorsal hippocampus and prelimbic cortex, a PFC subregion, at baseline, 1 h, and 6 d after episodic learning in rats. We found that, at baseline, dorsal hippocampus and prelimbic cortex differentially express a significant portion of mRNAs. Moreover, learning produces a transient regulation of region-specific profiles of mRNA, indicating that unique biological programs in different brain regions underlie memory formation., (Copyright © 2021 the authors.)
- Published
- 2021
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17. The Ontogeny of Hippocampus-Dependent Memories.
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Donato F, Alberini CM, Amso D, Dragoi G, Dranovsky A, and Newcombe NS
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- Adverse Childhood Experiences psychology, Animals, Hippocampus metabolism, Humans, Infant, Infant, Newborn, Memory physiology, Nerve Net metabolism, Child Development physiology, Hippocampus growth & development, Memory, Episodic, Nerve Net growth & development
- Abstract
The formation of memories that contain information about the specific time and place of acquisition, which are commonly referred to as "autobiographical" or "episodic" memories, critically relies on the hippocampus and on a series of interconnected structures located in the medial temporal lobe of the mammalian brain. The observation that adults retain very few of these memories from the first years of their life has fueled a long-standing debate on whether infants can make the types of memories that in adults are processed by the hippocampus-dependent memory system, and whether the hippocampus is involved in learning and memory processes early in life. Recent evidence shows that, even at a time when its circuitry is not yet mature, the infant hippocampus is able to produce long-lasting memories. However, the ability to acquire and store such memories relies on molecular pathways and network-based activity dynamics different from the adult system, which mature with age. The mechanisms underlying the formation of hippocampus-dependent memories during infancy, and the role that experience exerts in promoting the maturation of the hippocampus-dependent memory system, remain to be understood. In this review, we discuss recent advances in our understanding of the ontogeny and the biological correlates of hippocampus-dependent memories., (Copyright © 2021 the authors.)
- Published
- 2021
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18. CIM6P/IGF-2 Receptor Ligands Reverse Deficits in Angelman Syndrome Model Mice.
- Author
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Cruz E, Descalzi G, Steinmetz A, Scharfman HE, Katzman A, and Alberini CM
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- Animals, Disease Models, Animal, Ligands, Mice, Receptor, IGF Type 2, Angelman Syndrome complications, Angelman Syndrome drug therapy, Autism Spectrum Disorder
- Abstract
Angelman syndrome (AS), a genetic disorder that primarily affects the nervous system, is characterized by delayed development, intellectual disability, severe speech impairment, and problems with movement and balance (ataxia). Most affected children also have recurrent seizures (epilepsy). No existing therapies are capable of comprehensively treating the deficits in AS; hence, there is an urgent need to identify new treatments. Here we show that insulin-like growth factor 2 (IGF-2) and mannose-6-phosphate (M6P), ligands of two independent binding sites of the cation-independent M6P/IGF-2 receptor (CIM6P/IGF-2R), reverse most major deficits of AS modeled in mice. Subcutaneous injection of IGF-2 or M6P in mice modeling AS restored cognitive impairments as assessed by measurements of contextual and recognition memories, motor deficits assessed by rotarod and hindlimb clasping, and working memory/flexibility measured by Y-maze. IGF-2 also corrected deficits in marble burying and significantly attenuated acoustically induced seizures. An observational battery of tests confirmed that neither ligand changed basic functions including physical characteristics, general behavioral responses, and sensory reflexes, indicating that they are relatively safe. Our data provide strong preclinical evidence that targeting CIM6P/IGF-2R is a promising approach for developing novel therapeutics for AS. LAY SUMMARY: There is no effective treatment for the neurodevelopmental disorder Angelman syndrome (AS). Using a validated AS mouse model, the Ube3a
m-/p+ , in this study we show that systemic administration of ligands of the cation independent mannose-6-phosphate receptor, also known as insulin-like growth factor 2 receptor (CIM6P/IGF-2R) reverses cognitive impairment, motor deficits, as well as seizures associated with AS. Thus, ligands that activate the CIM6P/IGF-2R may represent novel, potential therapeutic targets for AS., (© 2020 International Society for Autism Research and Wiley Periodicals LLC.)- Published
- 2021
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19. A role for CIM6P/IGF2 receptor in memory consolidation and enhancement.
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Yu XW, Pandey K, Katzman AC, and Alberini CM
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- Animals, Female, Hippocampus metabolism, Hippocampus physiology, Male, Memory, Long-Term physiology, Mental Recall physiology, Mice, Neurons metabolism, Neurons physiology, Open Field Test, Rats, Rats, Long-Evans, Receptor, IGF Type 2 metabolism, Memory Consolidation physiology, Receptor, IGF Type 2 physiology
- Abstract
Cation-independent mannose-6-phosphate receptor, also called insulin-like growth factor two receptor (CIM6P/IGF2R), plays important roles in growth and development, but is also extensively expressed in the mature nervous system, particularly in the hippocampus, where its functions are largely unknown. One of its major ligands, IGF2, is critical for long-term memory formation and strengthening. Using CIM6P/IGF2R inhibition in rats and neuron-specific knockdown in mice, here we show that hippocampal CIM6P/IGF2R is necessary for hippocampus-dependent memory consolidation, but dispensable for learning, memory retrieval, and reconsolidation. CIM6P/IGF2R controls the training-induced upregulation of de novo protein synthesis, including increase of Arc, Egr1, and c-Fos proteins, without affecting their mRNA induction. Hippocampal or systemic administration of mannose-6-phosphate, like IGF2, significantly enhances memory retention and persistence in a CIM6P/IGF2R-dependent manner. Thus, hippocampal CIM6P/IGF2R plays a critical role in memory consolidation by controlling the rate of training-regulated protein metabolism and is also a target mechanism for memory enhancement., Competing Interests: XY, KP, AK, CA No competing interests declared, (© 2020, Yu et al.)
- Published
- 2020
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20. Early life experiences selectively mature learning and memory abilities.
- Author
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Bessières B, Travaglia A, Mowery TM, Zhang X, and Alberini CM
- Subjects
- Animals, Behavior, Animal, Brain-Derived Neurotrophic Factor metabolism, Disks Large Homolog 4 Protein metabolism, Female, Hippocampus physiology, Male, Mice, Rats, Rats, Long-Evans, Receptors, AMPA metabolism, Synapses metabolism, Synaptophysin metabolism, Learning, Memory
- Abstract
The mechanisms underlying the maturation of learning and memory abilities are poorly understood. Here we show that episodic learning produces unique biological changes in the hippocampus of infant rats and mice compared to juveniles and adults. These changes include persistent neuronal activation, BDNF-dependent increase in the excitatory synapse markers synaptophysin and PSD-95, and significant maturation of AMPA receptor synaptic responses. Inhibition of PSD-95 induction following learning impairs both AMPA receptor response maturation and infantile memory, indicating that the synapse formation/maturation is necessary for creating infantile memories. Conversely, capturing the learning-induced changes by presenting a subsequent learning experience or by chemogenetic activation of the neural ensembles tagged by learning matures memory functional competence. This memory competence is selective for the type of experience encountered, as it transfers within similar hippocampus-dependent learning domains but not to other hippocampus-dependent types of learning. Thus, experiences in early life produce selective maturation of memory abilities.
- Published
- 2020
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21. Corticosterone administration targeting a hypo-reactive HPA axis rescues a socially-avoidant phenotype in scarcity-adversity reared rats.
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Perry RE, Rincón-Cortés M, Braren SH, Brandes-Aitken AN, Opendak M, Pollonini G, Chopra D, Raver CC, Alberini CM, Blair C, and Sullivan RM
- Subjects
- Adolescent, Animals, Child, Corticosterone pharmacology, Female, Humans, Male, Rats, Stress, Psychological, Corticosterone therapeutic use, Hypothalamo-Hypophyseal System physiology, Social Behavior
- Abstract
It is well-established that children from low-income, under-resourced families are at increased risk of altered social development. However, the biological mechanisms by which poverty-related adversities can "get under the skin" to influence social behavior are poorly understood and cannot be easily ascertained using human research alone. This study utilized a rodent model of "scarcity-adversity," which encompasses material resource deprivation (scarcity) and reduced caregiving quality (adversity), to explore how early-life scarcity-adversity causally influences social behavior via disruption of developing stress physiology. Results showed that early-life scarcity-adversity exposure increased social avoidance when offspring were tested in a social approach test in peri-adolescence. Furthermore, early-life scarcity-adversity led to blunted hypothalamic-pituitary-adrenal (HPA) axis activity as measured via adrenocorticotropic hormone (ACTH) and corticosterone (CORT) reactivity following the social approach test. Western blot analysis of brain tissue revealed that glucocorticoid receptor levels in the dorsal (but not ventral) hippocampus and medial prefrontal cortex were significantly elevated in scarcity-adversity reared rats following the social approach test. Finally, pharmacological repletion of CORT in scarcity-adversity reared peri-adolescents rescued social behavior. Our findings provide causal support that early-life scarcity-adversity exposure negatively impacts social development via a hypocorticosteronism-dependent mechanism, which can be targeted via CORT administration to rescue social behavior., (Published by Elsevier Ltd.)
- Published
- 2019
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22. Enhancing Executive Functions Through Social Interactions: Causal Evidence Using a Cross-Species Model.
- Author
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Perry RE, Braren SH, Rincón-Cortés M, Brandes-Aitken AN, Chopra D, Opendak M, Alberini CM, Sullivan RM, and Blair C
- Abstract
It has long been theorized that humans develop higher mental functions, such as executive functions (EFs), within the context of interpersonal interactions and social relationships. Various components of social interactions, such as interpersonal communication, perspective taking, and conforming/adhering to social rules, may create important (and perhaps even necessary) opportunities for the acquisition and continued practice of EF skills. Furthermore, positive and stable relationships facilitate the development and maintenance of EFs across the lifespan. However, experimental studies investigating the extent to which social experiences contribute causally to the development of EFs are lacking. Here, we present experimental evidence that social experiences and the acquisition of social skills influence the development of EFs. Specifically, using a rat model, we demonstrate that following exposure to early-life adversity, a socialization intervention causally improves working memory in peri-adolescence. Our findings combined with the broader literature promote the importance of cultivating social skills in support of EF development and maintenance across the lifespan. Additionally, cross-species research will provide insight into causal mechanisms by which social experiences influence cognitive development and contribute to the development of biologically sensitive interventions., (Copyright © 2019 Perry, Braren, Rincón-Cortés, Brandes-Aitken, Chopra, Opendak, Alberini, Sullivan and Blair.)
- Published
- 2019
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23. Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat basolateral amygdala.
- Author
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Bessières B, Jia M, Travaglia A, and Alberini CM
- Subjects
- Age Factors, Animals, Female, Male, Rats, Rats, Long-Evans, Basolateral Nuclear Complex growth & development, Basolateral Nuclear Complex metabolism, Myelin Sheath metabolism, Nerve Net growth & development, Nerve Net metabolism, Neuroglia metabolism, Neuronal Plasticity physiology, Synapses metabolism
- Abstract
The basolateral complex of amygdala (BLA) processes emotionally arousing aversive and rewarding experiences. The BLA is critical for acquisition and storage of threat-based memories and the modulation of the consolidation of arousing explicit memories, that is, the memories that are encoded and stored by the medial temporal lobe. In addition, in conjunction with the medial prefrontal cortex (mPFC), the BLA plays an important role in fear memory extinction. The BLA develops relatively early in life, but little is known about the molecular changes that accompany its development. Here, we quantified relative basal expression levels of sets of plasticity, synaptic, glia, and connectivity proteins in the rat BLA at various developmental ages: postnatal day 17 (PN17, infants), PN24 (juveniles), and PN80 (young adults). We found that the levels of activation markers of brain plasticity, including phosphorylation of CREB at Ser133, CamKIIα at Thr286, pERK1/pERK2 at Thr202/Tyr204, and GluA1 at Ser831 and Ser845, were significantly higher in infant and juvenile compared with adult brain. In contrast, age increase was accompanied by a significant augmentation in the levels of proteins that mark synaptogenesis and synapse maturation, such as synaptophysin, PSD95, SynCAM, GAD65, GAD67, and GluN2A/GluN2B ratio. Finally, we observed significant age-associated changes in structural markers, including MAP2, MBP, and MAG, suggesting that the structural connectivity of the BLA increases over time. The biological differences in the BLA between developmental ages compared with adulthood suggest the need for caution in extrapolating conclusions based on BLA-related brain plasticity and behavioral studies conducted at different developmental stages., (© 2019 Bessières et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2019
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24. Wilm's tumor 1 promotes memory flexibility.
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Mariottini C, Munari L, Gunzel E, Seco JM, Tzavaras N, Hansen J, Stern SA, Gao V, Aleyasin H, Sharma A, Azeloglu EU, Hodes GE, Russo SJ, Huff V, Birtwistle MR, Blitzer RD, Alberini CM, and Iyengar R
- Subjects
- Animals, Behavior, Animal physiology, CA1 Region, Hippocampal metabolism, Fear physiology, Long-Term Potentiation physiology, Male, Memory Disorders pathology, Mice, Mice, Knockout, Neuronal Plasticity physiology, Neurons physiology, Rats, Rats, Sprague-Dawley, Repressor Proteins genetics, WT1 Proteins, Hippocampus physiology, Memory physiology, Repressor Proteins metabolism
- Abstract
Under physiological conditions, strength and persistence of memory must be regulated in order to produce behavioral flexibility. In fact, impairments in memory flexibility are associated with pathologies such as post-traumatic stress disorder or autism; however, the underlying mechanisms that enable memory flexibility are still poorly understood. Here, we identify transcriptional repressor Wilm's Tumor 1 (WT1) as a critical synaptic plasticity regulator that decreases memory strength, promoting memory flexibility. WT1 is activated in the hippocampus following induction of long-term potentiation (LTP) or learning. WT1 knockdown enhances CA1 neuronal excitability, LTP and long-term memory whereas its overexpression weakens memory retention. Moreover, forebrain WT1-deficient mice show deficits in both reversal, sequential learning tasks and contextual fear extinction, exhibiting impaired memory flexibility. We conclude that WT1 limits memory strength or promotes memory weakening, thus enabling memory flexibility, a process that is critical for learning from new experiences.
- Published
- 2019
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25. Lactate from astrocytes fuels learning-induced mRNA translation in excitatory and inhibitory neurons.
- Author
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Descalzi G, Gao V, Steinman MQ, Suzuki A, and Alberini CM
- Subjects
- Animals, Avoidance Learning, Brain metabolism, Citric Acid Cycle, Gangliosides, Glycogenolysis, Hippocampus metabolism, Male, Memory Disorders metabolism, Memory, Episodic, Monocarboxylic Acid Transporters genetics, Pyruvic Acid metabolism, Rats, Rats, Long-Evans, Astrocytes metabolism, Lactic Acid metabolism, Memory Consolidation, Neurons metabolism, RNA, Messenger metabolism
- Abstract
Glycogenolysis and lactate transport from astrocytes to neurons is required for long-term memory formation, but the role of this lactate is poorly understood. Here we show that the Krebs cycle substrates pyruvate and ketone body B3HB can functionally replace lactate in rescuing memory impairment caused by inhibition of glycogenolysis or expression knockdown of glia monocarboxylate transporters (MCTs) 1 and 4 in the dorsal hippocampus of rats. In contrast, either metabolite is unable to rescue memory impairment produced by expression knockdown of MCT2, which is selectively expressed by neurons, indicating that a critical role of astrocytic lactate is to provide energy for neuronal responses required for long-term memory. These responses include learning-induced mRNA translation in both excitatory and inhibitory neurons, as well as expression of Arc/Arg3.1. Thus, astrocytic lactate acts as an energy substrate to fuel learning-induced de novo neuronal translation critical for long-term memory., Competing Interests: Competing interestsThe authors declare no competing interests.
- Published
- 2019
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26. Editorial: Neurobiological Models of Psychotherapy.
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Javanbakht A and Alberini CM
- Published
- 2019
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27. Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex.
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Jia M, Travaglia A, Pollonini G, Fedele G, and Alberini CM
- Subjects
- Animals, Blotting, Western, Female, Gene Expression Regulation, Developmental, Male, Neuroglia cytology, Neurons cytology, Neurons metabolism, Prefrontal Cortex cytology, Rats, Long-Evans, Neuroglia metabolism, Neuronal Plasticity physiology, Prefrontal Cortex growth & development, Prefrontal Cortex metabolism, Proteins metabolism, Synapses metabolism
- Abstract
The medial prefrontal cortex (mPFC) plays a critical role in complex brain functions including decision-making, integration of emotional, and cognitive aspects in memory processing and memory consolidation. Because relatively little is known about the molecular mechanisms underlying its development, we quantified rat mPFC basal expression levels of sets of plasticity, synaptic, glia, and connectivity proteins at different developmental ages. Specifically, we compared the mPFC of rats at postnatal day 17 (PN17), when they are still unable to express long-term contextual and spatial memories, to rat mPFC at PN24, when they have acquired the ability of long-term memory expression and finally to the mPFC of adult rats. We found that, with increased age, there are remarkable and significant decreases in markers of cell activation and significant increases in proteins that mark synaptogenesis and synapse maturation. Furthermore, we found significant changes in structural markers over the ages, suggesting that structural connectivity of the mPFC increases over time. Finally, the substantial biological difference in mPFC at different ages suggest caution in extrapolating conclusions from brain plasticity studies conducted at different developmental stages., (© 2018 Jia et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2018
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28. Astrocyte glycogen and lactate: New insights into learning and memory mechanisms.
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Alberini CM, Cruz E, Descalzi G, Bessières B, and Gao V
- Subjects
- Animals, Brain metabolism, Humans, Neurons metabolism, Astrocytes metabolism, Glycogen metabolism, Lactic Acid metabolism, Learning physiology, Memory physiology
- Abstract
Memory, the ability to retain learned information, is necessary for survival. Thus far, molecular and cellular investigations of memory formation and storage have mainly focused on neuronal mechanisms. In addition to neurons, however, the brain comprises other types of cells and systems, including glia and vasculature. Accordingly, recent experimental work has begun to ask questions about the roles of non-neuronal cells in memory formation. These studies provide evidence that all types of glial cells (astrocytes, oligodendrocytes, and microglia) make important contributions to the processing of encoded information and storing memories. In this review, we summarize and discuss recent findings on the critical role of astrocytes as providers of energy for the long-lasting neuronal changes that are necessary for long-term memory formation. We focus on three main findings: first, the role of glucose metabolism and the learning- and activity-dependent metabolic coupling between astrocytes and neurons in the service of long-term memory formation; second, the role of astrocytic glucose metabolism in arousal, a state that contributes to the formation of very long-lasting and detailed memories; and finally, in light of the high energy demands of the brain during early development, we will discuss the possible role of astrocytic and neuronal glucose metabolisms in the formation of early-life memories. We conclude by proposing future directions and discussing the implications of these findings for brain health and disease. Astrocyte glycogenolysis and lactate play a critical role in memory formation. Emotionally salient experiences form strong memories by recruiting astrocytic β2 adrenergic receptors and astrocyte-generated lactate. Glycogenolysis and astrocyte-neuron metabolic coupling may also play critical roles in memory formation during development, when the energy requirements of brain metabolism are at their peak., (© 2017 Wiley Periodicals, Inc.)
- Published
- 2018
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29. Mechanisms of critical period in the hippocampus underlie object location learning and memory in infant rats.
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Travaglia A, Steinmetz AB, Miranda JM, and Alberini CM
- Subjects
- Animals, Brain-Derived Neurotrophic Factor administration & dosage, Brain-Derived Neurotrophic Factor physiology, Female, Mental Recall physiology, Rats, Long-Evans, Receptors, N-Methyl-D-Aspartate metabolism, Critical Period, Psychological, Hippocampus physiology, Memory, Episodic, Spatial Learning physiology
- Abstract
Episodic memories in early childhood are rapidly forgotten, a phenomenon that is associated with "infantile amnesia," the inability of adults to remember early-life experiences. We recently showed that early aversive contextual memory in infant rats, which is in fact rapidly forgotten, is actually not lost, as reminders presented later in life reinstate a long-lasting and context-specific memory. We also showed that the formation of this infantile memory recruits in the hippocampus mechanisms typical of developmental critical periods. Here, we tested whether similar mechanisms apply to a nonaversive, hippocampal type of learning. We report that novel object location (nOL) learned at postnatal day 17 (PN17) undergoes the typical rapid forgetting of infantile learning. However, a later reminder reinstates memory expression. Furthermore, as for aversive experiences, nOL learning at PN17 engages critical period mechanisms in the dorsal hippocampus: it induces a switch in the GluN2A/2B-NMDA receptor ratio, and brain-derived neurotrophic factor injected bilaterally into the dorsal hippocampus immediately after training results in long-lasting memory expression. We conclude that in infancy the hippocampus plays a necessary role in processing episodic and contextual memories, including nonaversive ones, and matures through a developmental critical period., (© 2018 Travaglia et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2018
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30. NLGN1 and NLGN2 in the prefrontal cortex: their role in memory consolidation and strengthening.
- Author
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Katzman A and Alberini CM
- Subjects
- Animals, Humans, Models, Animal, Nerve Net physiology, Neurons metabolism, Prefrontal Cortex pathology, Cell Adhesion Molecules, Neuronal metabolism, Immunoglobulins metabolism, Membrane Proteins metabolism, Memory Consolidation physiology, Prefrontal Cortex metabolism
- Abstract
The prefrontal cortex (PFC) is critical for memory formation, but the underlying molecular mechanisms are poorly understood. Clinical and animal model studies have shown that changes in PFC excitation and inhibition are important for cognitive functions as well as related disorders. Here, we discuss recent findings revealing the roles of the excitatory and inhibitory synaptic proteins neuroligin 1 (NLGN1) and NLGN2 in the PFC in memory formation and modulation of memory strength. We propose that shifts in NLGN1 and NLGN2 expression in specific excitatory and inhibitory neuronal subpopulations in response to experience regulate the dynamic processes of memory consolidation and strengthening. Because excitatory/inhibitory imbalances accompany neuropsychiatric disorders in which strength and flexibility of representations play important roles, understanding these mechanisms may suggest novel therapies., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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31. Insulin-Like Growth Factor II Targets the mTOR Pathway to Reverse Autism-Like Phenotypes in Mice.
- Author
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Steinmetz AB, Stern SA, Kohtz AS, Descalzi G, and Alberini CM
- Subjects
- Animals, Insulin-Like Growth Factor II metabolism, Male, Mice, Phenotype, Receptor, IGF Type 2 metabolism, Signal Transduction drug effects, Signal Transduction physiology, Autism Spectrum Disorder metabolism, Insulin-Like Growth Factor II pharmacology, TOR Serine-Threonine Kinases metabolism
- Abstract
Autism spectrum disorder (ASD) is a developmental disability characterized by impairments in social interaction and repetitive behavior, and is also associated with cognitive deficits. There is no current treatment that can ameliorate most of the ASD symptomatology; thus, identifying novel therapies is urgently needed. We used male BTBR T
+ Itpr3tf /J (BTBR) mice, a model that reproduces most of the core behavioral phenotypes of ASD, to test the effects of systemic administration of insulin-like growth factor II (IGF-II), a polypeptide that crosses the blood-brain barrier and acts as a cognitive enhancer. We show that systemic IGF-II treatments reverse the typical defects in social interaction, cognitive/executive functions, and repetitive behaviors reflective of ASD-like phenotypes. In BTBR mice, IGF-II, via IGF-II receptor, but not via IGF-I receptor, reverses the abnormal levels of the AMPK-mTOR-S6K pathway and of active translation at synapses. Thus, IGF-II may represent a novel potential therapy for ASD. SIGNIFICANCE STATEMENT Currently, there is no effective treatment for autism spectrum disorder (ASD), a developmental disability affecting a high number of children. Using a mouse model that expresses most of the key core as well as associated behavioral deficits of ASD, that are, social, cognitive, and repetitive behaviors, we report that a systemic administration of the polypeptide insulin-like growth factor II (IGF-II) reverses all these deficits. The effects of IGF-II occur via IGF-II receptors, and not IGF-I receptors, and target both basal and learning-dependent molecular abnormalities found in several ASD mice models, including those of identified genetic mutations. We suggest that IGF-II represents a potential novel therapeutic target for ASD., (Copyright © 2018 the authors 0270-6474/18/371015-15$15.00/0.)- Published
- 2018
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32. Erratum: Infantile amnesia reflects a developmental critical period for hippocampal learning.
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Travaglia A, Bisaz R, Sweet ES, Blitzer RD, and Alberini CM
- Published
- 2017
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33. Infantile Amnesia: A Critical Period of Learning to Learn and Remember.
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Alberini CM and Travaglia A
- Subjects
- Aging, Animals, Evidence-Based Medicine, Humans, Infant, Infant, Newborn, Models, Neurological, Rats, Amnesia physiopathology, Critical Period, Psychological, Hippocampus physiopathology, Memory, Long-Term, Mental Recall, Nerve Net physiopathology
- Abstract
Infantile amnesia, the inability of adults to recollect early episodic memories, is associated with the rapid forgetting that occurs in childhood. It has been suggested that infantile amnesia is due to the underdevelopment of the infant brain, which would preclude memory consolidation, or to deficits in memory retrieval. Although early memories are inaccessible to adults, early-life events, such as neglect or aversive experiences, can greatly impact adult behavior and may predispose individuals to various psychopathologies. It remains unclear how a brain that rapidly forgets, or is not yet able to form long-term memories, can exert such a long-lasting and important influence. Here, with a particular focus on the hippocampal memory system, we review the literature and discuss new evidence obtained in rats that illuminates the paradox of infantile amnesia. We propose that infantile amnesia reflects a developmental critical period during which the learning system is learning how to learn and remember., (Copyright © 2017 the authors 0270-6474/17/375783-13$15.00/0.)
- Published
- 2017
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34. Direct dorsal hippocampal-prelimbic cortex connections strengthen fear memories.
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Ye X, Kapeller-Libermann D, Travaglia A, Inda MC, and Alberini CM
- Subjects
- Animals, Brain-Derived Neurotrophic Factor metabolism, Conditioning, Classical physiology, Extinction, Psychological physiology, Male, Models, Animal, Rats, Long-Evans, Fear physiology, Hippocampus physiology, Memory physiology, Mental Recall physiology, Prefrontal Cortex physiology
- Abstract
The ability to regulate the consolidation and strengthening of memories for threatening experiences is critical for mental health, and its dysregulation may lead to psychopathologies. Re-exposure to the context in which the threat was experienced can either increase or decrease fear response through distinct processes known, respectively, as reconsolidation or extinction. Using a context retrieval-dependent memory-enhancement model in rats, we report that memory strengthens through activation of direct projections from dorsal hippocampus to prelimbic (PL) cortex and activation of critical PL molecular mechanisms that are not required for extinction. Furthermore, while sustained PL brain-derived neurotrophic factor (BDNF) expression is required for memory consolidation, retrieval engages PL BDNF to regulate excitatory and inhibitory synaptic proteins neuroligin 1 and neuroligin 2, which promote memory strengthening while inhibiting extinction. Thus, context retrieval-mediated fear-memory enhancement results from a concerted action of mechanisms that strengthen memory through reconsolidation while suppressing extinction., Competing Interests: The authors claim no conflict of interest.
- Published
- 2017
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35. Computational model of a positive BDNF feedback loop in hippocampal neurons following inhibitory avoidance training.
- Author
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Zhang Y, Smolen P, Alberini CM, Baxter DA, and Byrne JH
- Subjects
- Animals, Avoidance Learning drug effects, CCAAT-Enhancer-Binding Protein-beta metabolism, Cyclic AMP Response Element-Binding Protein metabolism, Feedback, Physiological drug effects, Gene Expression drug effects, Hippocampus drug effects, Neurons drug effects, Neurons metabolism, Phosphorylation, Protein Synthesis Inhibitors pharmacology, RNA, Messenger metabolism, Rats, Signal Transduction drug effects, Time Factors, Avoidance Learning physiology, Brain-Derived Neurotrophic Factor metabolism, Computer Simulation, Feedback, Physiological physiology, Hippocampus metabolism, Models, Neurological
- Abstract
Inhibitory avoidance (IA) training in rodents initiates a molecular cascade within hippocampal neurons. This cascade contributes to the transition of short- to long-term memory (i.e., consolidation). Here, a differential equation-based model was developed to describe a positive feedback loop within this molecular cascade. The feedback loop begins with an IA-induced release of brain-derived neurotrophic factor (BDNF), which in turn leads to rapid phosphorylation of the cAMP response element-binding protein (pCREB), and a subsequent increase in the level of the β isoform of the CCAAT/enhancer binding protein (C/EBPβ). Increased levels of C/EBPβ lead to increased bdnf expression. Simulations predicted that an empirically observed delay in the BDNF-pCREB-C/EBPβ feedback loop has a profound effect on the dynamics of consolidation. The model also predicted that at least two independent self-sustaining signaling pathways downstream from the BDNF-pCREB-C/EBPβ feedback loop contribute to consolidation. Currently, the nature of these downstream pathways is unknown., (© 2016 Zhang et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2016
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36. Developmental changes in plasticity, synaptic, glia and connectivity protein levels in rat dorsal hippocampus.
- Author
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Travaglia A, Bisaz R, Cruz E, and Alberini CM
- Subjects
- Age Factors, Animals, Female, Male, Rats, Rats, Long-Evans, Behavior, Animal physiology, Hippocampus growth & development, Hippocampus metabolism, Memory physiology, Neuroglia metabolism, Neuronal Plasticity physiology, Synapses metabolism
- Abstract
Thus far the identification and functional characterization of the molecular mechanisms underlying synaptic plasticity, learning, and memory have not been particularly dissociated from the contribution of developmental changes. Brain plasticity mechanisms have been largely identified and studied using in vitro systems mainly derived from early developmental ages, yet they are considered to be general plasticity mechanisms underlying functions -such as long-term memory- that occurs in the adult brain. Although it is possible that part of the plasticity mechanisms recruited during development is then re-recruited in plasticity responses in adulthood, systematic investigations about whether and how activity-dependent molecular responses differ over development are sparse. Notably, hippocampal-dependent memories are expressed relatively late in development, and the hippocampus undergoes and extended developmental post-natal structural and functional maturation, suggesting that the molecular mechanisms underlying hippocampal neuroplasticity may actually significantly change over development. Here we quantified the relative basal expression levels of sets of plasticity, synaptic, glia and connectivity proteins in rat dorsal hippocampus, a region that is critical for the formation of long-term explicit memories, at two developmental ages, postnatal day 17 (PN17) and PN24, which correspond to a period of relative functional immaturity and maturity, respectively, and compared them to adult age. We found that the levels of numerous proteins and/or their phosphorylation, known to be critical for synaptic plasticity underlying memory formation, including immediate early genes (IEGs), kinases, transcription factors and AMPA receptor subunits, peak at PN17 when the hippocampus is not yet able to express long-term memory. It remains to be established if these changes result from developmental basal activity or infantile learning. Conversely, among all markers investigated, the phosphorylation of calcium calmodulin kinase II α (CamKII α and of extracellular signal-regulated kinases 2 (ERK-2), and the levels of GluA1 and GluA2 significantly increase from PN17 to PN24 and then remain similar in adulthood, thus representing correlates paralleling long-term memory expression ability., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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37. Infantile amnesia reflects a developmental critical period for hippocampal learning.
- Author
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Travaglia A, Bisaz R, Sweet ES, Blitzer RD, and Alberini CM
- Subjects
- Animals, Brain-Derived Neurotrophic Factor metabolism, Female, Male, Nerve Net growth & development, Rats, Long-Evans, Receptors, N-Methyl-D-Aspartate metabolism, Amnesia physiopathology, Hippocampus growth & development, Learning physiology, Memory physiology
- Abstract
Episodic memories formed during the first postnatal period are rapidly forgotten, a phenomenon known as 'infantile amnesia'. In spite of this memory loss, early experiences influence adult behavior, raising the question of which mechanisms underlie infantile memories and amnesia. Here we show that in rats an experience learned during the infantile amnesia period is stored as a latent memory trace for a long time; indeed, a later reminder reinstates a robust, context-specific and long-lasting memory. The formation and storage of this latent memory requires the hippocampus, follows a sharp temporal boundary and occurs through mechanisms typical of developmental critical periods, including the expression switch of the NMDA receptor subunits from 2B to 2A, which is dependent on brain-derived neurotrophic factor (BDNF) and metabotropic glutamate receptor 5 (mGluR5). Activating BDNF or mGluR5 after training rescues the infantile amnesia. Thus, early episodic memories are not lost but remain stored long term. These data suggest that the hippocampus undergoes a developmental critical period to become functionally competent., Competing Interests: The authors declare no competing financial interests.
- Published
- 2016
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38. Insulin-like growth factor 2 rescues aging-related memory loss in rats.
- Author
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Steinmetz AB, Johnson SA, Iannitelli DE, Pollonini G, and Alberini CM
- Subjects
- Animals, Hippocampus metabolism, Insulin-Like Growth Factor II deficiency, Insulin-Like Growth Factor II metabolism, Male, Memory, Short-Term, Molecular Targeted Therapy, Proprotein Convertase 2 metabolism, Protein Processing, Post-Translational, Rats, Rats, Inbred F344, Recombinant Proteins administration & dosage, Aging psychology, Insulin-Like Growth Factor II administration & dosage, Insulin-Like Growth Factor II physiology, Memory, Memory Disorders etiology, Memory Disorders prevention & control
- Abstract
Aging is accompanied by declines in memory performance, and particularly affects memories that rely on hippocampal-cortical systems, such as episodic and explicit. With aged populations significantly increasing, the need for preventing or rescuing memory deficits is pressing. However, effective treatments are lacking. Here, we show that the level of the mature form of insulin-like growth factor 2 (IGF-2), a peptide regulated in the hippocampus by learning, required for memory consolidation and a promoter of memory enhancement in young adult rodents, is significantly reduced in hippocampal synapses of aged rats. By contrast, the hippocampal level of the immature form proIGF-2 is increased, suggesting an aging-related deficit in IGF-2 processing. In agreement, aged compared to young adult rats are deficient in the activity of proprotein convertase 2, an enzyme that likely mediates IGF-2 posttranslational processing. Hippocampal administration of the recombinant, mature form of IGF-2 rescues hippocampal-dependent memory deficits and working memory impairment in aged rats. Thus, IGF-2 may represent a novel therapeutic avenue for preventing or reversing aging-related cognitive impairments., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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39. Astrocytic β2-adrenergic receptors mediate hippocampal long-term memory consolidation.
- Author
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Gao V, Suzuki A, Magistretti PJ, Lengacher S, Pollonini G, Steinman MQ, and Alberini CM
- Subjects
- Adrenergic beta-Antagonists administration & dosage, Adrenergic beta-Antagonists pharmacology, Analysis of Variance, Animals, Hippocampus drug effects, Hippocampus metabolism, Lactic Acid metabolism, Lactic Acid pharmacology, Learning physiology, Male, Memory, Long-Term drug effects, Propanolamines administration & dosage, Propanolamines pharmacology, Propranolol administration & dosage, Propranolol pharmacology, RNA Interference, Rats, Long-Evans, Receptors, Adrenergic, beta-2 genetics, Time Factors, Astrocytes metabolism, Hippocampus physiology, Memory, Long-Term physiology, Receptors, Adrenergic, beta-2 metabolism
- Abstract
Emotionally relevant experiences form strong and long-lasting memories by critically engaging the stress hormone/neurotransmitter noradrenaline, which mediates and modulates the consolidation of these memories. Noradrenaline acts through adrenergic receptors (ARs), of which β2-adrenergic receptors (βARs) are of particular importance. The differential anatomical and cellular distribution of βAR subtypes in the brain suggests that they play distinct roles in memory processing, although much about their specific contributions and mechanisms of action remains to be understood. Here we show that astrocytic rather than neuronal β2ARs in the hippocampus play a key role in the consolidation of a fear-based contextual memory. These hippocampal β2ARs, but not β1ARs, are coupled to the training-dependent release of lactate from astrocytes, which is necessary for long-term memory formation and for underlying molecular changes. This key metabolic role of astrocytic β2ARs may represent a novel target mechanism for stress-related psychopathologies and neurodegeneration.
- Published
- 2016
- Full Text
- View/download PDF
40. Effective anti-Alzheimer Aβ therapy involves depletion of specific Aβ oligomer subtypes.
- Author
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Knight EM, Kim SH, Kottwitz JC, Hatami A, Albay R, Suzuki A, Lublin A, Alberini CM, Klein WL, Szabo P, Relkin NR, Ehrlich M, Glabe CG, Gandy S, and Steele JW
- Abstract
Background: Recent studies have implicated specific assembly subtypes of β-amyloid (Aβ) peptide, specifically soluble oligomers (soAβ) as disease-relevant structures that may underlie memory loss in Alzheimer disease. Removing existing soluble and insoluble Aβ assemblies is thought to be essential for any attempt at stabilizing brain function and slowing cognitive decline in Alzheimer disease. IV immunoglobulin (IVIg) therapies have been shown to contain naturally occurring polyclonal antibodies that recognize conformational neoepitopes of soluble or insoluble Aβ assemblies including soAβ. These naturally occurring polyclonal antibodies have been suggested to underlie the apparent clinical benefits of IVIg. However, direct evidence linking anti-Aβ antibodies to the clinical bioactivity of IVIg has been lacking., Methods: Five-month-old female Dutch APP E693Q mice were treated for 3 months with neat IVIg or with IVIg that had been affinity-depleted over immobilized Aβ conformers in 1 of 2 assembly states. Memory was assessed in a battery of tests followed by quantification of brain soAβ levels using standard anti-soAβ antibodies., Results: We provide evidence that NU4-type soAβ (NU4-soAβ) assemblies accumulate in the brains of Dutch APP E693Q mice and are associated with defects in memory, even in the absence of insoluble Aβ plaques. Memory benefits were associated with depletion from APP E693Q mouse brain of NU4-soAβ and A11-soAβ but not OC-type fibrillar Aβ oligomers., Conclusions: We propose that targeting of specific soAβ assembly subtypes may be an important consideration in the therapeutic and/or prophylactic benefit of anti-Aβ antibody drugs.
- Published
- 2016
- Full Text
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41. The Role of Lactate-Mediated Metabolic Coupling between Astrocytes and Neurons in Long-Term Memory Formation.
- Author
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Steinman MQ, Gao V, and Alberini CM
- Abstract
Long-term memory formation, the ability to retain information over time about an experience, is a complex function that affects multiple behaviors, and is an integral part of an individual's identity. In the last 50 years many scientists have focused their work on understanding the biological mechanisms underlying memory formation and processing. Molecular studies over the last three decades have mostly investigated, or given attention to, neuronal mechanisms. However, the brain is composed of different cell types that, by concerted actions, cooperate to mediate brain functions. Here, we consider some new insights that emerged from recent studies implicating astrocytic glycogen and glucose metabolisms, and particularly their coupling to neuronal functions via lactate, as an essential mechanism for long-term memory formation.
- Published
- 2016
- Full Text
- View/download PDF
42. From Memory Impairment to Posttraumatic Stress Disorder-Like Phenotypes: The Critical Role of an Unpredictable Second Traumatic Experience.
- Author
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Finsterwald C, Steinmetz AB, Travaglia A, and Alberini CM
- Subjects
- AIDS-Related Complex metabolism, Animals, Avoidance Learning drug effects, Brain-Derived Neurotrophic Factor metabolism, CREB-Binding Protein metabolism, Corticosterone metabolism, Corticosterone pharmacology, Disease Models, Animal, Dose-Response Relationship, Drug, Electroshock adverse effects, Exploratory Behavior physiology, Generalization, Psychological, Hippocampus metabolism, Male, Methyl-CpG-Binding Protein 2, Rats, Rats, Long-Evans, Receptor, trkB metabolism, Memory Disorders etiology, Phenotype, Stress Disorders, Post-Traumatic complications
- Abstract
Arousal and stress critically regulate memory formation and retention. Increasing levels of stress produce an inverted U-shaped effect on cognitive performance, including the retention of explicit memories, and experiencing a severe stress during a traumatic event may lead to posttraumatic stress disorder (PTSD). The molecular mechanisms underlying the impairing effect of a severe stress on memory and the key contribution of traumatic experiences toward the development of PTSD are still unknown. Here, using increasing footshock intensities in an inhibitory avoidance paradigm, we reproduced the inverted U-shaped curve of memory performance in rats. We then show that the inverted U profile of memory performance correlates with an inverted U profile of corticosterone level in the circulation and of brain-derived neurotrophic factor, phosphorylated tropomyosin-receptor kinase B, and methyl CpG binding protein in the dorsal hippocampus. Furthermore, training with the highest footshock intensity (traumatic experience) led to a significant elevation of hippocampal glucocorticoid receptors. Exposure to an unpredictable, but not to a predictable, highly stressful reminder shock after a first traumatic experience resulted in PTSD-like phenotypes, including increased memory of the trauma, high anxiety, threat generalization, and resistance to extinction. Systemic corticosterone injection immediately after the traumatic experience, but not 3 d later, was sufficient to produce PTSD-like phenotypes. We suggest that, although after a first traumatic experience a suppression of the corticosterone-dependent response protects against the development of an anxiety disorder, experiencing more than one trauma (multiple hits) is a critical contributor to the etiology of PTSD., (Copyright © 2015 the authors 0270-6474/15/3515903-13$15.00/0.)
- Published
- 2015
- Full Text
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43. Insulin Like Growth Factor 2 Expression in the Rat Brain Both in Basal Condition and following Learning Predominantly Derives from the Maternal Allele.
- Author
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Ye X, Kohtz A, Pollonini G, Riccio A, and Alberini CM
- Subjects
- Alleles, Animals, Avoidance Learning physiology, Base Sequence, Crosses, Genetic, DNA Methylation, Female, Gene Expression Regulation, Hippocampus chemistry, Insulin-Like Growth Factor II metabolism, Kidney chemistry, Liver chemistry, Male, Memory, Long-Term physiology, Molecular Sequence Data, Prefrontal Cortex chemistry, RNA, Messenger metabolism, Rats, Rats, Inbred F344, Sequence Alignment, Sex Factors, Spleen chemistry, Genomic Imprinting, Hippocampus physiology, Insulin-Like Growth Factor II genetics, Prefrontal Cortex physiology, RNA, Messenger genetics
- Abstract
Insulin like growth factor 2 (Igf2) is known as a maternally imprinted gene involved in growth and development. Recently, Igf2 was found to also be regulated and required in the adult rat hippocampus for long-term memory formation, raising the question of its allelic regulation in adult brain regions following experience and in cognitive processes. We show that, in adult rats, Igf2 is abundantly expressed in brain regions involved in cognitive functions, like hippocampus and prefrontal cortex, compared to the peripheral tissues. In contrast to its maternal imprinting in peripheral tissues, Igf2 is mainly expressed from the maternal allele in these brain regions. The training-dependent increase in Igf2 expression derives proportionally from both parental alleles, and, hence, is mostly maternal. Thus, Igf2 parental expression in the adult rat brain does not follow the imprinting rules found in peripheral tissues, suggesting differential expression regulation and functions of imprinted genes in the brain.
- Published
- 2015
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44. VGF and Its C-Terminal Peptide TLQP-62 Regulate Memory Formation in Hippocampus via a BDNF-TrkB-Dependent Mechanism.
- Author
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Lin WJ, Jiang C, Sadahiro M, Bozdagi O, Vulchanova L, Alberini CM, and Salton SR
- Subjects
- Animals, Avoidance Learning, Brain cytology, Conditioning, Psychological physiology, Down-Regulation genetics, Enzyme Activators pharmacology, Enzyme Inhibitors pharmacology, Excitatory Postsynaptic Potentials drug effects, Excitatory Postsynaptic Potentials genetics, Flavanones pharmacology, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, In Vitro Techniques, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Nerve Growth Factors, Neurons physiology, Neuropeptides genetics, Peptides metabolism, Rats, Rats, Long-Evans, Receptor, trkB antagonists & inhibitors, Brain metabolism, Brain-Derived Neurotrophic Factor metabolism, Memory physiology, Neuropeptides metabolism, Peptides administration & dosage, Receptor, trkB metabolism
- Abstract
Regulated expression and secretion of BDNF, which activates TrkB receptor signaling, is known to play a critical role in cognition. Identification of additional modulators of cognitive behavior that regulate activity-dependent BDNF secretion and/or potentiate TrkB receptor signaling would therefore be of considerable interest. In this study, we show in the adult mouse hippocampus that expression of the granin family gene Vgf and secretion of its C-terminal VGF-derived peptide TLQP-62 are required for fear memory formation. We found that hippocampal VGF expression and TLQP-62 levels were transiently induced after fear memory training and that sequestering secreted TLQP-62 peptide in the hippocampus immediately after training impaired memory formation. Reduced VGF expression was found to impair learning-evoked Rac1 induction and phosphorylation of the synaptic plasticity markers cofilin and synapsin in the adult mouse hippocampus. Moreover, TLQP-62 induced acute, transient activation of the TrkB receptor and subsequent CREB phosphorylation in hippocampal slice preparations and its administration immediately after training enhanced long-term memory formation. A critical role of BDNF-TrkB signaling as a downstream effector in VGF/TLQP-62-mediated memory consolidation was further revealed by posttraining activation of BDNF-TrkB signaling, which rescued impaired fear memory resulting from hippocampal administration of anti-VGF antibodies or germline VGF ablation in mice. We propose that VGF is a critical component of a positive BDNF-TrkB regulatory loop and, upon its induced expression by memory training, the TLQP-62 peptide rapidly reinforces BDNF-TrkB signaling, regulating hippocampal memory consolidation., Significance Statement: Identification of the cellular and molecular mechanisms that regulate long-term memory formation and storage may provide alternative treatment modalities for degenerative and neuropsychiatric memory disorders. The neurotrophin BDNF plays a prominent role in cognitive function, and rapidly and robustly induces expression of VGF, a secreted neuronal peptide precursor. VGF knock-out mice have impaired fear and spatial memory. Our study shows that VGF and VGF-derived peptide TLQP-62 are transiently induced after fear memory training, leading to increased BDNF/TrkB signaling, and that sequestration of hippocampal TLQP-62 immediately after training impairs memory formation. We propose that TLQP-62 is a critical component of a positive regulatory loop that is induced by memory training, rapidly reinforces BDNF-TrkB signaling, and is required for hippocampal memory consolidation., (Copyright © 2015 the authors 0270-6474/15/3510344-14$15.00/0.)
- Published
- 2015
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45. Commentary on Tuch.
- Author
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Alberini CM
- Subjects
- Humans, Neurosciences, Psychoanalysis
- Published
- 2015
- Full Text
- View/download PDF
46. The regulation of transcription in memory consolidation.
- Author
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Alberini CM and Kandel ER
- Subjects
- Humans, RNA, Untranslated metabolism, Transcription Factors metabolism, Chromatin metabolism, Epigenesis, Genetic physiology, Gene Expression Regulation physiology, Memory, Long-Term physiology, Models, Neurological, Neuronal Plasticity physiology, Transcription, Genetic physiology
- Abstract
De novo transcription of DNA is a fundamental requirement for the formation of long-term memory. It is required during both consolidation and reconsolidation, the posttraining and postreactivation phases that change the state of the memory from a fragile into a stable and long-lasting form. Transcription generates both mRNAs that are translated into proteins, which are necessary for the growth of new synaptic connections, as well as noncoding RNA transcripts that have regulatory or effector roles in gene expression. The result is a cascade of events that ultimately leads to structural changes in the neurons that mediate long-term memory storage. The de novo transcription, critical for synaptic plasticity and memory formation, is orchestrated by chromatin and epigenetic modifications. The complexity of transcription regulation, its temporal progression, and the effectors produced all contribute to the flexibility and persistence of long-term memory formation. In this article, we provide an overview of the mechanisms contributing to this transcriptional regulation underlying long-term memory formation., (Copyright © 2015 Cold Spring Harbor Laboratory Press; all rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
47. Editorial. Molecular and Cellular Cognition Society (MCCS) meetings.
- Author
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Alberini CM, Josselyn S, and Tsai LH
- Subjects
- Animals, Brain physiology, Congresses as Topic, Humans, Molecular Biology, Cognition physiology
- Published
- 2014
- Full Text
- View/download PDF
48. Insulin-like growth factor 2 reverses memory and synaptic deficits in APP transgenic mice.
- Author
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Pascual-Lucas M, Viana da Silva S, Di Scala M, Garcia-Barroso C, González-Aseguinolaza G, Mulle C, Alberini CM, Cuadrado-Tejedor M, and Garcia-Osta A
- Subjects
- Aged, Alzheimer Disease genetics, Alzheimer Disease metabolism, Amyloid beta-Protein Precursor genetics, Animals, Blotting, Western, Cell Line, Cell Line, Tumor, Cells, Cultured, Dendritic Spines genetics, Dendritic Spines physiology, Dependovirus genetics, Disease Models, Animal, Female, Genetic Vectors genetics, HEK293 Cells, Hippocampus metabolism, Hippocampus physiopathology, Humans, Insulin-Like Growth Factor I genetics, Insulin-Like Growth Factor I metabolism, Insulin-Like Growth Factor II genetics, Male, Memory Disorders genetics, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Confocal, Reverse Transcriptase Polymerase Chain Reaction, Synaptic Transmission genetics, Alzheimer Disease physiopathology, Insulin-Like Growth Factor II metabolism, Memory Disorders physiopathology, Synaptic Transmission physiology
- Abstract
Insulin-like growth factor 2 (IGF2) was recently found to play a critical role in memory consolidation in rats and mice, and hippocampal or systemic administration of recombinant IGF2 enhances memory. Here, using a gene therapy-based approach with adeno-associated virus (AAV), we show that IGF2 overexpression in the hippocampus of aged wild-type mice enhances memory and promotes dendritic spine formation. Furthermore, we report that IGF2 expression decreases in the hippocampus of patients with Alzheimer's disease, and this leads us to hypothesize that increased IGF2 levels may be beneficial for treating the disease. Thus, we used the AAV system to deliver IGF2 or IGF1 into the hippocampus of the APP mouse model Tg2576 and demonstrate that IGF2 and insulin-like growth factor 1 (IGF1) rescue behavioural deficits, promote dendritic spine formation and restore normal hippocampal excitatory synaptic transmission. The brains of Tg2576 mice that overexpress IGF2 but not IGF1 also show a significant reduction in amyloid levels. This reduction probably occurs through an interaction with the IGF2 receptor (IGF2R). Hence, IGF2 and, to a lesser extent, IGF1 may be effective treatments for Alzheimer's disease., (© 2014 The Authors. Published under the terms of the CC BY 4.0 license.)
- Published
- 2014
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49. The effect of insulin and insulin-like growth factors on hippocampus- and amygdala-dependent long-term memory formation.
- Author
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Stern SA, Chen DY, and Alberini CM
- Subjects
- Animals, Avoidance Learning drug effects, Conditioning, Psychological drug effects, Fear drug effects, Male, Rats, Rats, Long-Evans, Amygdala drug effects, Hippocampus drug effects, Insulin pharmacology, Insulin-Like Growth Factor I pharmacology, Insulin-Like Growth Factor II pharmacology, Memory, Long-Term drug effects, Nootropic Agents pharmacology
- Abstract
Recent work has reported that the insulin-like growth factor 2 (IGF2) promotes memory enhancement. Furthermore, impaired insulin or IGF1 functions have been suggested to play a role in the pathogenesis of neurodegeneration and cognitive impairments, hence implicating the insulin/IGF system as an important target for cognitive enhancement and/or the development of novel treatments against cognitive disorders. Here, we tested the effect of intracerebral injections of IGF1, IGF2, or insulin on memory consolidation and persistence in rats. We found that a bilateral injection of insulin into the dorsal hippocampus transiently enhances hippocampal-dependent memory and an injection of IGF1 has no effect. None of the three peptides injected into the amygdala affected memories critically engaging this region. Together with previous data on IGF2, these results indicate that IGF2 produces the most potent and persistent effect as a memory enhancer on hippocampal-dependent memories. We suggest that the memory-enhancing effects of insulin and IGF2 are likely mediated by distinct mechanisms., (© 2014 Stern et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2014
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50. A positive autoregulatory BDNF feedback loop via C/EBPβ mediates hippocampal memory consolidation.
- Author
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Bambah-Mukku D, Travaglia A, Chen DY, Pollonini G, and Alberini CM
- Subjects
- Animals, Feedback, Physiological physiology, Male, Rats, Rats, Long-Evans, Signal Transduction physiology, Avoidance Learning physiology, Brain-Derived Neurotrophic Factor metabolism, CCAAT-Enhancer-Binding Protein-beta metabolism, Hippocampus physiology, Inhibition, Psychological, Memory, Long-Term physiology
- Abstract
Little is known about the temporal progression and regulation of the mechanisms underlying memory consolidation. Brain-derived-neurotrophic-factor (BDNF) has been shown to mediate the maintenance of memory consolidation, but the mechanisms of this regulation remain unclear. Using inhibitory avoidance (IA) in rats, here we show that a hippocampal BDNF-positive autoregulatory feedback loop via CCAAT-enhancer binding protein β (C/EBPβ) is necessary to mediate memory consolidation. At training, a very rapid, learning-induced requirement of BDNF accompanied by rapid de novo translation controls the induction of a persistent activation of cAMP-response element binding-protein (CREB) and C/EBPβ expression. The latter, in turn, controls an increase in expression of bdnf exon IV transcripts and BDNF protein, both of which are necessary and, together with the initial BDNF requirement, mediate memory consolidation. The autoregulatory loop terminates by 48 h after training with decreased C/EBPβ and pCREB and increased methyl-CpG binding protein-2, histone-deacetylase-2, and switch-independent-3a binding at the bdnf exon IV promoter., (Copyright © 2014 the authors 0270-6474/14/3412547-13$15.00/0.)
- Published
- 2014
- Full Text
- View/download PDF
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