Search

Your search keyword '"Albane Marie"' showing total 6 results
Start Over Author "Albane Marie"
6 results on '"Albane Marie"'

1. The mosquito microbiome includes habitat-specific but rare symbionts

Author

Hans Schrieke, Loïs Maignien, Florentin Constancias, Florian Trigodet, Sarah Chakloute, Ignace Rakotoarivony, Albane Marie, Gregory L'Ambert, Patrick Makoundou, Nonito Pages, A. Murat Eren, Mylène Weill, Mathieu Sicard, and Julie Reveillaud

Subjects
Microbiome, Mosquitoes, Symbionts, Microdiversity, Oligotyping, Biotechnology, TP248.13-248.65
Abstract

Microbial communities are known to influence mosquito lifestyles by modifying essential metabolic and behavioral processes that affect reproduction, development, immunity, digestion, egg survival, and the ability to transmit pathogens. Many studies have used 16S rRNA gene amplicons to characterize mosquito microbiota and investigate factors that influence host-microbiota dynamics. However, a relatively low taxonomic resolution due to clustering methods based on arbitrary threshold and the overall dominance of Wolbachia or Asaia symbionts obscured the investigation of rare members of mosquito microbiota in previous studies. Here, we used high resolution Shannon entropy-based oligotyping approach to analyze the microbiota of Culex pipiens, Culex quinquefasciatus and Aedes individuals from continental Southern France and overseas Guadeloupe as well as from laboratories with or without antibiotics treatment. Our experimental design that resulted in a series of mosquito samples with a gradient of Wolbachia density and relative abundance along with high-resolution analyses of amplicon sequences enabled the recovery of a robust signal from typically less accessible bacterial taxa. Our data confirm species-specific mosquito-bacteria associations with geography as a primary factor that influences bacterial community structure. But interestingly, they also reveal co-occurring symbiotic bacterial variants within single individuals for both Elizabethkingia and Erwinia genera, distinct and specific Asaia and Chryseobacterium in continental and overseas territories, and a putative rare Wolbachia variant. Overall, our study reveals the presence of previously overlooked microdiversity and multiple closely related symbiotic strains within mosquito individuals with a remarkable habitat-specificity.

Published
2022
Full Text
View/download PDF

2. Spatial scale influences taxon conservation in the eukaryotic virome of a mosquito

Author

Patricia Gil, Antoni Exbrayat, Etienne Loire, Ignace Rakotoarivony, Florian Charriat, Thierry Baldet, Michel Boisseau, Albane Marie, Benoît Frances, Gregory L’Ambert, Mohamed Bessat, Yehia Otify, Maria Goffredo, Giuseppe Mancini, Núria Busquets-Martí, Lotty Birnberg, Sandra Talavera, Carlos Aranda, Emna Ayari, Selma Mejri, Soufien Sghaier, Amal Bennouna, Hicham El Rhaffouli, Thomas Balenghien, Ghita Chlyeh, Ouafaa Fassi Fihri, Julie Reveillaud, Yannick Simonin, Marc Eloit, and Serafin Gutierrez

Abstract

Our knowledge on the diversity of eukaryotic viruses has recently undergone a massive expansion. This diversity could influence host physiology through yet unknown phenomena of potential interest to the fields of health and food production. However, the assembly processes in eukaryotic viromes of terrestrial animals remain elusive. This situation hinders hypothesis-driven tests of virome influence on host physiology. Here, we explore virome assembly at different spatial scales in the eukaryotic virome of the mosquitoCulex pipiens. This mosquito is a vector of human pathogens worldwide. Several new aspects of virome assembly were unveiled through a sampling involving five countries in Africa and Europe and large sample sizes. A group of viruses was conserved inC. pipienspopulations in all countries. This core group represented a relatively large and diverse fraction of the virome. However, core viruses were not shared by all host individuals in a given country, and their infection rates fluctuated between countries and years. Moreover, the distribution of co-infections in individual mosquitoes suggested random co-occurrence of certain core viruses. We also observed differences in the virome depending on geography, with viromes tending to cluster depending on the continent. Thus, our results unveil that taxon conservation in a eukaryotic virome changes with spatial scale. Thus, predictions on virome assembly seem possible at a large geographical scale inC. pipiens.IMPORTANCEThe study of the eukaryotic virome of mosquitoes is an emerging research field. Beyond its fundamental interest, this field could lead to the development of control tools against the transmission of mosquito-borne human pathogens. However, we yet know little on the assembly patterns in the eukaryotic viromes of mosquitoes, as well as of terrestrial animals in general. This situation hampers the design of hypothesis-driven studies on the influence of the virome on pathogen transmission. Here, we have analyzed virome assembly in the mosquito vectorCulex pipienswithin and between countries in Africa and Europe. Our results show that integrating contrasted spatial scales allows to identify deterministic patterns in virome assembly. Such patterns can guide future studies of virome influence on mosquito physiology.

Published
2022

3. One Health surveillance of West Nile and Usutu viruses

Author

Orianne Constant, Patricia Gil, Jonathan Barthelemy, Karine Bolloré, Vincent Foulongne, Caroline Desmetz, Agnès Leblond, Isabelle Desjardins, Sophie Pradier, Aurélien Joulié, Alain Sandoz, Rayane Amaral, Michel Boisseau, Ignace Rakotoarivony, Thierry Baldet, Albane Marie, Benoît Frances, Florence Reboul Salze, Bachirou Tinto, Philippe Van de Perre, Sara Salinas, Cécile Beck, Sylvie Lecollinet, Serafin Gutierrez, Yannick Simonin, Pathogenesis and Control of Chronic and Emerging Infections (PCCEI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA)-Etablissement français du don du sang [Montpellier]-Université de Montpellier (UM), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Biocommunication en Cardio-Métabolique (BC2M), Université de Montpellier (UM), Unité Mixte de Recherche d'Épidémiologie des maladies Animales et zoonotiques (UMR EPIA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), GREMERES ICE, Clinique Vétérinaire Jolimont, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Laboratoire Chimie de l'environnement (LCE), Aix Marseille Université (AMU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Entente interdépartementale pour la démoustication du littoral méditerranéen [Montpellier] (EID Méditerranée), EID-Méditerranée, This study was funded by the ANR (French National Research Agency, reference ANR-16-IDEX-0006), the KIM RIVE initiative, the Labex EpiGenMed 'Investissements d’avenir' programme (reference ANR- 10-LABX-12-01), and Montpellier University of Excellence (MUSE, ArboSud project). Moreover, SG was recipient of funding from the Direction Générale de l’Alimentation from the French Ministry of Agriculture and Food. Horse serosurveys were funded by the French Horse and Riding Institute, IFCE, and the EPERON fund (Encouragement aux ProjetsEquestres Régionaux Ou Nationaux) (OpIUM project)., ANR-16-IDEX-0006,MUSE,MUSE(2016), and ANR-10-LABX-0012,EpiGenMed,From Genome and Epigenome to Molecular Medicine: turning new paradigms in biology into the therapeutic strategies of tomorrow(2010)

Subjects
Epidemiology, viruses, [SDV]Life Sciences [q-bio], L73 - Maladies des animaux, Flavivirus Infections, Virus des animaux, Birds, Dogs, Endémie, Seroepidemiologic Studies, Virology, Animals, Humans, Surveillance épidémiologique, Horses, One Health, Usutu virus, Transmission des maladies, seroprevalence, virus du Nil occidental, Flavivirus, Public Health, Environmental and Occupational Health, virus diseases, Morbidité, Cross-Sectional Studies, Culicidae, S50 - Santé humaine, France, One Health surveillance, West Nile virus, West Nile Fever, co-circulation
Abstract

Background West Nile virus (WNV) and Usutu virus (USUV), two closely related flaviviruses, mainly follow an enzootic cycle involving mosquitoes and birds, but also infect humans and other mammals. Since 2010, their epidemiological situation may have shifted from irregular epidemics to endemicity in several European regions; this requires confirmation, as it could have implications for risk assessment and surveillance strategies. Aim To explore the seroprevalence in animals and humans and potential endemicity of WNV and USUV in Southern France, given a long history of WNV outbreaks and the only severe human USUV case in France in this region. Methods We evaluated the prevalence of WNV and USUV in a repeated cross-sectional study by serological and molecular analyses of human, dog, horse, bird and mosquito samples in the Camargue area, including the city of Montpellier, between 2016 and 2020. Results We observed the active transmission of both viruses and higher USUV prevalence in humans, dogs, birds and mosquitoes, while WNV prevalence was higher in horses. In 500 human samples, 15 were positive for USUV and 6 for WNV. Genetic data showed that the same lineages, WNV lineage 1a and USUV lineage Africa 3, were found in mosquitoes in 2015, 2018 and 2020. Conclusion These findings support existing literature suggesting endemisation in the study region and contribute to a better understanding of USUV and WNV circulation in Southern France. Our study underlines the importance of a One Health approach for the surveillance of these viruses.

Published
2022

4. A library preparation optimized for metagenomics of RNA viruses

Author

Biram Biteye, Marc Eloit, Ignace Rakotoarivony, Thomas Balenghien, Claire Garros, Rachid Koual, Patricia Gil, Virginie Dupuy, Serafin Gutierrez, Antoni Exbrayat, Assane Gueye Fall, Grégory L'Ambert, Emmanuel Albina, Etienne Loire, Geoffrey Gimonneau, Albane Marie, Benoit Francés, Julie Reveillaud, Momar Talla Seck, Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Laboratoire National d'Elevage et de Recherches Vétérinaires [Dakar] (LNERV), Institut Sénégalais de Recherches Agricoles [Dakar] (ISRA), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), Entente Interdépartementale pour la démoustication du littoral méditerranéen (EID), École nationale vétérinaire - Alfort (ENVA), Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP), Centre Collaborateur de l'OIE de Détection et identification chez l’homme des pathogènes animaux émergents et développement d’outils pour leur diagnostic / Collaborating Center for the Detection and identification in humans of emerging animal pathogens and development of tools for their diagnoses (CCOIE-OIECC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé Animale / World Organisation Animal Health [Paris] (OIE), This work was funded by the Direction Générale de l’Alimentation from the French Ministry in charge of agriculture. This work was also funded by the European Union’s Seventh Framework Programme through grants Vmerge (FP7‐613996) and CuliOme (FP7‐291815)., European Project: 291815,EC:FP7:KBBE,FP7-ERANET-2011-RTD,ANIHWA(2012), European Project: 613996,EC:FP7:KBBE,FP7-KBBE-2013-7-single-stage,VMERGE(2013), European Project: 7247564(1972), Université de Bordeaux (UB)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), École nationale vétérinaire d'Alfort (ENVA), Institut Pasteur [Paris], and Institut Pasteur [Paris]-Organisation Mondiale de la Santé Animale / World Animal Health Information System (OIE-WAHIS)

Subjects
0106 biological sciences, 0301 basic medicine, Viral metagenomics, [SDV]Life Sciences [q-bio], Banque de gènes, Caulimovirus mosaïque du chou fleur, L73 - Maladies des animaux, 01 natural sciences, Genome, shotgun sequencing, Aedes vexans, Culex pipiens, insects, viromevirus, education.field_of_study, Shotgun sequencing, Virome, [SDV.BA]Life Sciences [q-bio]/Animal biology, Culicoides, PCR, Vecteur de maladie, S50 - Santé humaine, L72 - Organismes nuisibles des animaux, Biotechnology, Population, Computational biology, Genome, Viral, Biology, 010603 evolutionary biology, génomique, 03 medical and health sciences, Virus bluetongue, Genetics, Animals, RNA Viruses, Human virome, education, Ecology, Evolution, Behavior and Systematics, Virus classification, Gene Library, metagenomics, Virus maladie de newcastle, Bacteriome, 030104 developmental biology, Metagenomics, Metagenome, library preparation
Abstract

International audience; Our understanding of the viral communities associated to animals has not yet reached the level attained on the bacteriome. This situation is due to, among others, technical challenges in adapting metagenomics using high-throughput sequencing to the study of RNA viromes in animals. Although important developments have been achieved in most steps of viral metagenomics, there is yet a key step that has received little attention: the library preparation. This situation differs from bacteriome studies in which developments in library preparation have largely contributed to the democratisation of metagenomics. Here, we present a library preparation optimized for metagenomics of RNA viruses from insect vectors of viral diseases. The library design allows a simple PCR-based preparation, such as those routinely used in bacterial metabarcoding, that is adapted to shotgun sequencing as required in viral metagenomics. We first optimized our library preparation using mock viral communities and then validated a full metagenomic approach incorporating our preparation in two pilot studies with field-caught insect vectors; one including a comparison with a published metagenomic protocol. Our approach provided a fold increase in virus-like sequences compared to other studies, and nearly-full genomes from new virus species. Moreover, our results suggested conserved trends in virome composition within a population of a mosquito species. Finally, the sensitivity of our approach was compared to a commercial diagnostic PCR for the detection of an arbovirus in field-caught insect vectors. Our approach could facilitate studies on viral communities from animals and the democratization of metagenomics in community ecology of viruses.

Published
2021

5. Emergence of two Usutu virus lineages in Culex pipiens mosquitoes in the Camargue, France, 2015

Author

Martin H. Groschup, Yannick Simonin, Benoit Francés, Ute Ziegler, Serafin Gutierrez, Marc Eloit, Martin Eiden, Patricia Gil, Ignace Rakotoarivony, Albane Marie, Vincent Foulongne, Grégory L'Ambert, Friedrich-Loeffler-Institut (FLI), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), EID Méditerranée (EID Méditerranée), Etablissement public administratif, Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Laboratoire de Virologie, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), European Project: 613996,EC:FP7:KBBE,FP7-KBBE-2013-7-single-stage,VMERGE(2013), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), and Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
0301 basic medicine, Microbiology (medical), Zoology, Mosquito population, L73 - Maladies des animaux, Microbiology, Virus, Flavivirus Infections, 03 medical and health sciences, Mosquito, Culex pipiens, parasitic diseases, Genetics, Animals, Usutu virus, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Phylogeny, Lineages Africa 2/3, biology, Phylogenetic tree, Flavivirus, fungi, 000 - Autres thèmes, biology.organism_classification, 3. Good health, Culex, France, arbovirus, 030104 developmental biology, Infectious Diseases, RNA, Viral, Female, France, L72 - Organismes nuisibles des animaux, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience; Usutu virus (USUV) was detected in 11 Culex pipiens mosquito pools collected in 2015 in Camargue (France) using quantitative real-time RT-PCR assays. Phylogenetic analysis of recovered virus sequences identified USUV lineages Africa 2 and Africa 3, demonstrating the simultaneous occurrence of different strains within the mosquito population. This is the first report on USUV in mosquitoes from France that concurrently accompanied the emergence of Usutu virus in blackbirds and a human case in France during 2015/2016.

Published
2018

6. Persistence and Recycling of Bioinsecticidal Bacillus thuringiensis subsp. israelensis Spores in Contrasting Environments: Evidence from Field Monitoring and Laboratory Experiments

Author

Claire Duchet, Christophe Lagneau, Laurence Després, Margot Paris, Delphine Rey, Albane Marie, Jean-Philippe David, Yvon Perrin, Guillaume Tetreau, Gilles Besnard, Écologie et santé des écosystèmes (ESE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire d'Ecologie Alpine (LECA), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Entente Interdépartementale pour la Démoustication du Littoral Méditerranéen, Centre national d'expertise des vecteurs, Montpellier (CNEV), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF), Institut National de L'Energie Solaire (INES), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Mécanique et Technologie (LMT), École normale supérieure - Cachan (ENS Cachan)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Aménagement, Développement, Environnement, Santé et Sociétés (ADES), Centre National de la Recherche Scientifique (CNRS)-Université Bordeaux Segalen - Bordeaux 2-Université Bordeaux Montaigne, Laboratoire de Biologie des Populations d'Altitude, Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), and Université Bordeaux Segalen - Bordeaux 2-Université Bordeaux Montaigne-Centre National de la Recherche Scientifique (CNRS)

Subjects
Mosquito Control, [SDV]Life Sciences [q-bio], Bacillus thuringiensis, Soil Science, Environment, recycling, Persistence (computer science), mosquito larvae, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, Botany, parasitic diseases, Animals, Organic matter, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, Spores, Bacterial, Bacillus thuringiensis subsp israelensis, geography, geography.geographical_feature_category, Ecology, biology, fungi, persistence, 15. Life on land, biology.organism_classification, field, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Spore, Salinity, Horticulture, Mosquito control, Culicidae, chemistry, Brackish marsh, Larva, [SDE]Environmental Sciences, France, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Microcosm
Abstract

Sprays of commercial preparations of the bacterium Bacillus thuringiensis subsp. israelensis are widely used for the control of mosquito larvae. Despite an abundant literature on B. thuringiensis subsp. israelensis field efficiency on mosquito control, few studies have evaluated the fate of spores in the environment after treatments. In the present article, two complementary experiments were conducted to study the effect of different parameters on B. thuringiensis subsp. israelensis persistence and recycling, in field conditions and in the laboratory. First, we monitored B. thuringiensis subsp. israelensis persistence in the field in two contrasting regions in France: the Rhone-Alpes region, where mosquito breeding sites are temporary ponds under forest cover with large amounts of decaying leaf matter on the ground and the Mediterranean region characterized by open breeding sites such as brackish marshes. Viable B. thuringiensis subsp. israelensis spores can persist for months after a treatment, and their quantity is explained both by the vegetation type and by the number of local treatments. We found no evidence of B. thuringiensis subsp. israelensis recycling in the field. Then, we tested the effect of water level, substrate type, salinity and presence of mosquito larvae on the persistence/recycling of B. thuringiensis subsp. israelensis spores in controlled laboratory conditions (microcosms). We found no effect of change in water level or salinity on B. thuringiensis subsp. israelensis persistence over time (75 days). B. thuringiensis subsp. israelensis spores tended to persist longer in substrates containing organic matter compared to sand-only substrates. B. thuringiensis subsp. israelensis recycling only occurred in presence of mosquito larvae but was unrelated to the presence of organic matter.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results