Search

BACKGROUND: PSMA expression is influenced by hormonal status. We evaluated changes in PSA and whole-body 68Ga-PSMA-11 PET/CT (WB-PSMA PET) after initiation of androgen receptor signaling inhibitors (ARSi). METHODS: Prospectively enrolled patients with metastatic castration-resistant prostate cancer (mCRPC) initiating ARSi underwent serial PSA measurements and WB-PSMA PET at baseline, 1-week, and 3-months post-ARSi. We correlated WB-PSMA PET metrics and PSA kinetics after ARSi to 1-year clinical outcome. RESULTS: Due to low enrollment rate, the study was closed before reaching the recruitment goal of 30 patients. Nine patients were enrolled. At 1-year, unfavorable outcome was documented in 6/9 (66%) patients. Nine/9 patients completed PSMA PET at 1-week, 5/9 at 3-months. Changes in PSA, PSMA-VOL, SUVmean and SUVmax were - 12%, + 5%, + 3%, and + 10% at 1-week, - 42%, - 16%, - 15% and - 17% at 3-months, respectively. CONCLUSIONS: Our prospective trial involving 9 mCRPC patients initiating ARSi did not show significant modulation of PSMA expression measured on WB-PSMA PET at 1-week. This study was registered on clinicaltrials.gov (NCT04279561).

Various programs to fulfill community nutrition by the government in Sukodono District have been actively implemented to alleviate the problem of stunting. Our service activities are based on strategic issues faced in Sukodono District, consisting of education regarding the causes and solutions to the stunting problem and socializing one of the efforts to eradicate stunting. Supported by the Lumajang Regency Government, it has made various efforts to handle and alleviate stunting in Lumajang, so this outreach activity provides insight to the community, especially posyandu cadres, prospective brides, and mothers. This activity aims to help the communities in the Sukodono District, Lumajang Regency, to eradicate the problem of stunting by socializing the tilapia fish farming model and the benefits of tilapia fish. The activity is community service through socialization, which can increase public understanding before and after presenting material on tilapia program activities to prevent stunting.

Tilapia are commonly used in aquaculture but are sensitive to cold, limiting their culture in colder regions. Thus, the purpose of this investigation was to examine how lecithin and/or Arabic gum could help Nile tilapia cope with cold stress during the winter season. In a 3×3 factorial feeding study, tilapia fingerlings with an average initial weight of 7.56 ± 0.10 g were given nine different diets (crude protein: 305 g kg−1; gross energy: 19.47 MJ kg−1) for 60 days during the winter. The diet formulations included three levels of Arabic gum (0 g, 2 g, and 4 g kg−1), with each level receiving three different dosages of lecithin (0 g, 5 g, and 10 g kg−1). After the trial, tilapia fed the food enriched with 4 g kg−1 Arabic gum and 10 g kg−1 lecithin showed the highest specific growth rate, fish survival, weight gain, and the lowest feed conversion ratio. Low-density lipoprotein cholesterol and cholesterol levels were highest in fish given the control diet. The groups fed diets with 10 g kg−1 lecithin and 4 g kg−1 Arabic gum showed higher levels of triglycerides and HDL-C. Significant decreases in alanine aminotransferase, glucose, aspartate aminotransferase, and cortisol activities were also observed (P < 0.05) with the same diet. Fish fed a diet enriched with 4 g kg−1 Arabic gum and 10 g kg−1 lecithin showed the highest levels of serum calcium, potassium, sodium, and chlorine, as well as the highest transcription of Δ9D. The same diet was shown to have the lowest levels of malondialdehyde and the highest activity levels for other antioxidant enzymes such as glutathione peroxides, superoxide dismutase, glutathione, catalase, and total antioxidant capacity. Tilapia raised in cold-stressed winter conditions had improved survival and performance when fed a diet containing 4 g kg−1 of Arabic gum and 10 g kg−1 of lecithin.

ABSTRACT Objective: To evaluate the impact of chronic illness caused by chikungunya fever on the quality of life and functionality of affected individuals. Methods: A cross-sectional and comparative study was conducted in which two groups were investigated: a Chikungunya Group comprising 25 patients with chronic fever screened after 1 year of illness via a telephonic survey, and a Healthy Group comprising 25 healthy individuals matched for sex and age by face-to-face interview. The Stanford HAQ 20-Item Disability Scale (HAQ) and the Short Form Health Survey (SF-12) questionnaires were administered to both groups. Generalized Linear Models, Pearson χ2 tests, and odds ratios were used to evaluate the test results. Results: Significant differences in functional capacity and quality of life were observed between the Chikungunya and Healthy Groups. The chance of some impairment in functionality was also much higher in the Chikungunya Group in four of the HAQ categories, especially in the "walking" category (adjusted OR= 109.40). Further, the Chikungunya Group had a higher chance of presenting a below-average score in the mental component summary of the SF-12 (adjusted OR= 16.20) and of being positive in depression risk screening (adjusted OR= 34.57). Conclusion: Even one year after the acute phase, chikungunya fever can compromise the functionality and quality of life in affected individuals, with direct impairment in performing daily activities. Studies and therapeutic plans for chikungunya fever should consider the long-term impacts of this disease.

Background: Basic sanitation services and practices are crucial for the well-being and health of a community. However, little is known about the determinants of basic sanitation services in Ethiopia, particularly in the study area. Objectives: To identify the determinants of basic sanitation service coverage among households in the Shashogo district, Hadiya Zone, and Central Ethiopia in 2023. Methods: A case–control investigation was carried out in households in Shashogo District from August 8, 2023, to December 10, 2023. Ten Kebeles were chosen randomly from a total of 34 Kebeles in the district. A total of 579 households (193 cases and 386 controls) were included in the study. The data were gathered with pretested and structured questionnaires in digital form with a smartphone. The data were analyzed with SPSS. Independent t tests and regression models were used to determine predictors of basic sanitation services. Results: The mean (±SD) age of the study participants was 38.2 (±8.9) years. After controlling for confounders in the multivariate analysis, the adoption of improved latrine services among households was found to be associated with greater average monthly income (AOR = 1.841, 95% CI (1.043, 3.251)), the study respondents who had access to the necessary materials for latrine construction/upgrade were approximately 3.5 times more likely to adopt improved latrine services among households with (AOR = 3.477 (95% CI = 1.314, 9.202) than among those who lacked access to the necessary materials for latrine construction, the study respondents who had training in sanitation and hygiene (AOR = 4.744, 95% CI (2.984, 7.542)) and who had sufficient knowledge about the adoption of improved latrine services (AOR = 2.020, 95% CI (1.125, 3.628)) were found to be determinants of the adoption of improved latrine services among households. Conclusion: This study revealed that economics, knowledge, attitudes and practices, infrastructure, and culture were identified as determinants of the adoption of improved latrines in the study area. Poor sanitation significantly impacts economic, social, environmental and many other direct and indirect impacts on the well-being of citizens. Therefore, policies on sanitation and contextual initiatives and focused actions are needed by stakeholders and policy implementers to improve knowledge gaps, access to necessary materials and the income capacity of those lagging to adopt services.

This study proposes a novel approach to address the challenges posed by the increasing number of renewable sources in power grids, particularly focusing on networked microgrids. The proposed method integrates digital twin technology and blockchain technology to provide power support in networked microgrids. The method comprises three main components: 1) Blockchain Network: A blockchain network is established, utilizing a modified proof of stake (PoS) consensus mechanism. This network is responsible for maintaining hourly records and tracking system changes, including faults. Fault events are recorded in real-time, and a bidding mechanism is employed to determine which microgrids interconnect and provide power support to mitigate power imbalances. 2) Digital Twins: Digital twins are created for each distributed generation and energy storage unit within the microgrids. These digital twins play a crucial role in the proposed PoS consensus mechanism, providing an additional layer of validation for transaction records. 3) Long short-term memory (LSTM)-based Controller: An LSTM-based controller is implemented to ensure stable operation of the microgrids, even during fault events and interconnections with other microgrids. The controller is trained to maintain stable voltage levels within the system. The digital twins and LSTM-based controllers are optimized using Bayesian Optimization to determine the best parameters and hyperparameters. Simulation results demonstrate that the proposed method effectively records hourly system details and fault events. Additionally, power hardware-in-the-loop simulations confirm that the LSTM-based controller can maintain stable voltage levels and outperforms other controllers in terms of performance.

Tilapia culture is one of the largest sectors of global aquaculture. Among the different species of tilapia, Nile tilapia (Oreochromis niloticus) is perhaps the top cultured species. The production of Nile tilapia has been continually increasing throughout the years resulting in genetic deterioration. Several tilapia strains with better growth performance and adaptive capability to survive in different culture conditions have been developed to alleviate the crisis. Increased demand for Nile tilapia implies higher farming cost. Plant-based proteins are utilized as partial or complete fishmeal replacements to reduce feed cost. However, these proteins can adversely affect and alter growth and feed performance, carcass composition and indices, and gut and hepatic health. This review discusses the use of seven plant-based proteins: namely, soybean, copra, pea, corn, palm kernel, microalgae, and seaweed as a Nile tilapia aquafeed. Different processing methods are employed to produce several types of plant-based proteins. Processed plant-protein types, when utilized as an aquafeed ingredient, vary in its effect on the performance, hemato-immunological parameters, and gut and hepatic health of Nile tilapia. Studies have shown that Nile tilapia can effectively maximize plant-based protein diets based on the preparation method, type of plant source, amino acid supplementation, and inclusion levels of the plant proteins. These readily available crops should be considered as primary protein sources for aquaculture. Hindrances to the use of plant-based proteins as a main dietary protein are limiting amino acids, presence of anti-nutritional factors, and the competition between its demand as human food and as animal feed.

Abstract Climate change is rapidly altering weather patterns, resulting in shifts in climatic zones. The survival of trees in specific locations depends on their functional traits. Local populations exhibit trait adaptations that ensure their survival and accomplishment of growth and reproduction processes during the growing season. Studying these traits offers valuable insights into species responses to present and future environmental conditions, aiding the implementation of measures to ensure forest resilience and productivity. This study investigates the variability in functional traits among five black spruce (Picea mariana (Mill.) B.S.P.) provenances originating from a latitudinal gradient along the boreal forest, and planted in a common garden in Quebec, Canada. We examined differences in bud phenology, growth performance, lifetime first reproduction, and the impact of a late-frost event on tree growth and phenological adjustments. The findings revealed that trees from northern sites exhibit earlier budbreak, lower growth increments, and reach reproductive maturity earlier than those from southern sites. Late-frost damage affected growth performance, but no phenological adjustment was observed in the successive year. Local adaptation in the functional traits may lead to maladaptation of black spruce under future climate conditions or serve as a potent evolutionary force promoting rapid adaptation under changing environmental conditions.

Within rowing, lower back disorders (LBDs) are common, but the mechanisms underpinning LBDs are poorly understood. Considering this, it is essential to understand how coordination and motor control change under different constraints such as ergometer rowing and fatigue. This can help better inform movement features linked to LBDs. Measurement of the continuous relative phase (CRP) is a method used to quantify body segment and joint coordination, as CRP measures the spatiotemporal control of multi-joint movement. The purpose of this study was twofold: to examine the general spatiotemporal coordination aspects of ergometer rowing in an unfatigued state, and to quantify how the spatiotemporal coordination of a rowing movement changes in response to a fatigue-inducing rowing trial. Wearable IMUs monitored 20 participants’ movement during a 2000 m ergometer row. The Borg-10 Rating of Perceived Exertion (RPE) scale was used to quantify perceived fatigue. Despite significant RPE increases across all athletes, the spatiotemporal coordination of rowing revealed prevailing strategies for the lumbar spine and lower extremity but no significant effects (α = 0.05) of fatigue on CRP outcomes (MARP, DP), cross-correlation lag (RXY), or range of motion. These findings provide further insight into rowing movements and support the idea that heterogeneous responses to fatigue may exist, requiring further study.

Abstract Background PSMA expression is influenced by hormonal status. We evaluated changes in PSA and whole-body 68Ga-PSMA-11 PET/CT (WB-PSMA PET) after initiation of androgen receptor signaling inhibitors (ARSi). Methods Prospectively enrolled patients with metastatic castration-resistant prostate cancer (mCRPC) initiating ARSi underwent serial PSA measurements and WB-PSMA PET at baseline, 1-week, and 3-months post-ARSi. We correlated WB-PSMA PET metrics and PSA kinetics after ARSi to 1-year clinical outcome. Results Due to low enrollment rate, the study was closed before reaching the recruitment goal of 30 patients. Nine patients were enrolled. At 1-year, unfavorable outcome was documented in 6/9 (66%) patients. Nine/9 patients completed PSMA PET at 1-week, 5/9 at 3-months. Changes in PSA, PSMA-VOL, SUVmean and SUVmax were − 12%, + 5%, + 3%, and + 10% at 1-week, − 42%, − 16%, − 15% and − 17% at 3-months, respectively. Conclusions Our prospective trial involving 9 mCRPC patients initiating ARSi did not show significant modulation of PSMA expression measured on WB-PSMA PET at 1-week. This study was registered on clinicaltrials.gov (NCT04279561).

Abstract Background Physical activity has been shown to benefit patients undergoing adjuvant cancer therapy. Although exercise interventions may be applied in several settings, most trials have focused on specialized facilities for their interventions. While these approaches benefit the access for individuals living near exercise centers, it hampers the assessment of real-world effectiveness. Therefore, evaluating the feasibility and implementation of home-based models of exercise training, especially in low-to-middle-income settings, may inform future physical activity trials and programs. In this article, we present the protocol for the BENEFIT CA trial, which aims to assess the implementation of a remote exercise intervention for patients with breast cancer or prostate cancer, primarily quantifying adherence to an exercise program. Methods This is a 12-week study, utilizing a non-randomized, single-arm design to assess the feasibility of a home-based exercise training. The intervention is remotely guided, and participants also receive an educational component about cancer and exercise. The study aims to recruit 40 patients diagnosed with breast cancer and 40 patients diagnosed with prostate cancer, all of whom undergoing active hormonal treatment. The primary outcome is the level of adherence, indicated as the proportion of performed exercise episodes. Secondary outcomes include recruitment rates, fatigue, quality of life, and functional capacity. Adverse events will be monitored throughout the study. Because this is a feasibility trial, the statistical analysis plan is based on descriptive statistics, which encompasses an intention-to-treat analysis and a plan for handling missing data. Discussion This is a low-cost feasibility study to orient the design of a wide-range, pragmatic phase 3 trial based on remote exercise intervention. With this study, we aim to better understand the adherence and implementation strategies regarding home-based exercise for the proposed population and, in the near future, move forward to a randomized clinical trial. In addition, this trial may contribute to engage patients with cancer in exercise programs throughout their treatment and beyond. Trial registration This trial has been approved by the Hospital de Clínicas de Porto Alegre Ethics Committee/IRB (48,869,621.9.0000.5327), and it is registered at Clinicaltrials.gov (NCT05258526), registered on February 25, 2022, prior to the beginning of the study.

The prostate-specific membrane antigen (PSMA) has been targeted for PET imaging and radioligand therapy (RLT) in patients with prostate cancer. Xerostomia is a common side effect of RLT because of the high salivary gland uptake of PSMA radioligands. Here, we aimed to determine the impact of monosodium glutamate (MSG) administration on PSMA-radioligand biodistribution within healthy organs and tumor lesions by using 68Ga-PSMA-11 PET imaging. Methods: Sixteen men with prostate cancer were randomized (1:1) into oral ingestion and oral topical application ("swishing") arms. Each subject underwent 2 68Ga-PSMA-11 PET/CT scans within 14 d under baseline and MSG conditions. The salivary glands and whole-body tumor lesions were segmented using qPSMA software. We quantified tracer uptake via SUVmean and SUVmax and compared parameters within each patient. Results: For the oral ingestion arm, salivary gland SUVmean and SUVmax decreased on average from the control scan to the MSG scan by 45% ± 15% (P = 0.004) and 53% ± 11% (P < 0.001), respectively. Tumor lesion SUVmean and SUVmax also decreased by 38% (interquartile range, -67% to -33%) and -52% (interquartile range, -70% to -49%), respectively (P = 0.018). Swishing had no significant effect on 68Ga-PSMA-11 accumulation in normal organs or tumor lesions. Conclusion: Oral ingestion but not topical application of MSG reduced 68Ga-PSMA-11 uptake in salivary glands. Tumor uptake also declined; therefore, the clinical application of MSG is unlikely to be useful in the framework of RLT.

BackgroundThis study addresses the need for improved transparency and reproducibility in randomized clinical trials (RCTs) within the field of physical activity (PA) interventions. Despite efforts to promote these practices, there is limited evidence on the adherence to established reporting and methodological standards in published RCTs. The research, part of the Strengthening the Evidence in Exercise Sciences Initiative (SEES Initiative) in 2020, assessed the methodological standards and reporting quality of RCTs focusing on PA interventions.MethodsRCTs of PA advice or exercise interventions published in 2020 were selected. Monthly searches were conducted on PubMed/MEDLINE targeting six top-tier exercise science journals. Assessments were conducted by two independent authors, based on 44 items originally from CONSORT and TIDieR reporting guidelines. These items were divided into seven domains: transparency, completeness, participants, intervention, rigor methodology, outcomes and critical analysis. Descriptive analysis was performed using absolute and relative frequencies, and exploratory analysis was done by comparing proportions using the χ2 test (α = 0.05).ResultsOut of 1,766 RCTs evaluated for eligibility, 53 were included. The median adherence to recommended items across the studies was 30 (18-44) items in individual assessments. Notably, items demonstrating full adherence were related to intervention description, justification, outcome measurement, effect sizes, and statistical analysis. Conversely, the least reported item pertained to mentioning unplanned modifications during trials, appearing in only 11.3% of studies. Among the 53 RCTs, 67.9% reported having a registration, and these registered studies showed higher adherence to assessed items compared to non-registered ones.ConclusionsIn summary, while critical analysis aspects were more comprehensively described, aspects associated with transparency, such as protocol registrations/modifications and intervention descriptions, were reported suboptimally. The findings underscore the importance of promoting resources related to reporting quality and transparent research practices for investigators and editors in the exercise sciences discipline.

Abstract Tityus serrulatus scorpion is responsible for a significant number of envenomings in Brazil, ranging from mild to severe, and in some cases, leading to fatalities. While supportive care is the primary treatment modality, moderate and severe cases require antivenom administration despite potential limitations and adverse effects. The remarkable proliferation of T. serrulatus scorpions, attributed to their biology and asexual reproduction, contributes to a high incidence of envenomation. T. serrulatus scorpion venom predominantly consists of short proteins acting as neurotoxins (α and β), that primarily target ion channels. Nevertheless, high molecular weight compounds, including metalloproteases, serine proteases, phospholipases, and hyaluronidases, are also present in the venom. These compounds play a crucial role in envenomation, influencing the severity of symptoms and the spread of venom. This review endeavors to comprehensively understand the T. serrulatus scorpion venom by elucidating the primary high molecular weight compounds and exploring their potential contributions to envenomation. Understanding these compounds' mechanisms of action can aid in developing more effective treatments and prevention strategies, ultimately mitigating the impact of scorpion envenomation on public health in Brazil.

While some research supports utilizing plain radiography for measuring biomechanical alignment of the spine for prognosis and treatment, there are contrasting viewpoints regarding both the value and utilization of these procedures in conservative care. Evaluation of both conservative and non-conservative approaches to spinal care revealed vast differences in radiographic utilization and interpretation between orthopedic surgeons, primary care physicians, chiropractic physicians, and physical therapists, which may account for the different viewpoints and rationales in the literature. A research summary is provided to explore any unique biomechanical parameters identified with plain radiography of the spine (PROTS) and how these measurements may relate to patient health. Understanding any unique value provided through biomechanical assessment utilizing PROTS may help chiropractic physicians determine the appropriate use of radiographic procedures in clinical practice and how to coordinate efforts with other conservative and non-conservative spinal healthcare professions to improve patient health.

The impact of prostate-specific membrane antigen (PSMA) PET/CT on management of prostate cancer (PCa) patients with biochemical recurrence (BCR) is well established. However, whether and how PSMA PET/CT affects the management of patients undergoing scans for other clinical indications remains unknown. The goal of this study was to determine the impact of 68Ga-PSMA-11 PET/CT on initial and subsequent management decisions in a cohort of PCa patients referred for various indications (i.e., a basket trial) excluding the 2 main classic indications: BCR and presurgical staging. Methods: This was a prospective study of 197 patients that aimed to determine the impact of 68Ga-PSMA-11 PET/CT on PCa stage and management. The indications for PSMA PET/CT were initial staging of nonsurgical candidates (30 patients) and restaging after definitive treatment (167 patients). The restaging cohort comprised patients restaged with known advanced metastatic disease (n = 103), after androgen deprivation therapy only (n = 16), after surgery and with serum prostate-specific antigen levels lower than 0.2 ng/mL (n = 13), after radiation therapy and not meeting the Phoenix criteria (n = 22), and after other primary local treatments (i.e., high-intensity focused ultrasound, focal laser ablation, cryoablation, hyperthermia, or irreversible electroporation) (n = 13). Patients with BCR and candidates for curative surgery were excluded. Impact on management was assessed using pre- and post-PET questionnaires completed by referring physicians, electronic chart review, or patient telephone calls. Results: PSMA PET/CT changed the disease stage in 135 of 197 (69%) patients (upstaging in 38%, downstaging in 30%, and no change in stage in 32%). Management was affected in 104 of 182 (57%) patients. Specifically, PSMA PET/CT impacted the management of patients who were restaged after radiation therapy without meeting the Phoenix criteria for BCR, after other definitive local treatments, and with advanced metastatic disease in 13 of 18 (72%), 8 of 12 (67%), and 59 of 96 (61%), respectively. Conclusion: PSMA PET/CT has a profound impact on stage and management of PCa patients outside the 2 main classic indications (BCR and presurgical staging) across all examined clinical scenarios.

This work aimed to develop biodegradable composites formed from corn starch-based films and cellulose extracted from pineapple crown residue. Fibers obtained from the crowns underwent alkaline treatment, followed by bleaching. Cellulose was characterized by thermogravimetric analysis (TGA), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). These analyses verified that the process was effective in isolating cellulose fibers. The biocomposites were then produced via casting and with cellulose of different concentrations (5%, 10%, 15%). These biocomposites were less soluble in water than the pure starch film, and the film with 15% cellulose had lower water vapor permeability rate (WVPR) compared to pure starch film. In terms of the thermal characterization, there were no significant variations in the melting and gelatinization temperatures of the films. When analyzing the mechanical properties, it was seen that the Young’s modulus of the biocomposites increased in relation to the starch film, in which the composite with a 15% increase of 377.76%. Regarding the tensile strength, there were no differences between preferences, probably due to the dispersion of the fibers.

Com objetivo de comparar o desempenho agronômico da Tifton 85 com a Missioneira Gigante (Axonopus jesuiticus (Araújo) Valls), foi conduzido durante dois anos um trabalho de pesquisa na Epagri/Estação Experimental de Urussanga.

This current and pioneering work aimed to assess the on-road performance of selected hybrid electric vehicles (HEVs) and electric vehicles (EVs) in local urban road conditions following the World Harmonized Light Vehicles Test Procedure (WLTP) and the chase car protocol. An experimental research design was also implemented to investigate the effects of the different payload conditions on vehicle performance, and corresponding drive cycle patterns for the test vehicles were generated from each on-road test. From the series of these on-road tests, it was revealed that there was high variability in speed profiles, and vehicle speed was generally found to be inversely related to payload weight. The variations in the state of charge, fuel fill-up, and fuel and energy parameters exhibited no significant differences in terms of payload conditions. When compared to both the Canada fuel consumption guide and the US fuel consumption guide, the resulting fuel consumption and energy consumption indicated that the Mitsubishi Outlander PHEV and Mitsubishi iMiEV exceeded energy efficiency standards, unlike the Toyota Prius. Meanwhile, in terms of CO2 emissions, all vehicles demonstrated around 40–70% lower emissions compared to conventional vehicles according to the 2023 estimates of the US Environmental Protection Agency. Being the first of its kind in the Philippines, this study on the on-road performance assessments of HEVs and EVs is essential because it provides empirical data on these vehicles’ actual performance in everyday driving conditions. The data are important for evaluating the potential to address environmental concerns, promote sustainable transportation solutions, influence consumer adoption, and shape government policies. With ongoing improvements in technology and expanding charging infrastructure, HEVs and EVs are poised for significant adoption in the coming years.

Abstract Background Cannabinoid-based therapy has been shown to be promising and is emerging as crucial for the treatment of cognitive deficits, mental illnesses, and many diseases considered incurable. There is a need to find an appropriate therapy for Alzheimer’s disease, and cannabinoid-based therapy appears to be a feasible possibility. Case presentation This report addresses the beneficial effect of cannabinoids in microdoses on improving memory and brain functions of a patient with mild-stage Alzheimer’s disease. The patient is a 75-year-old white man presenting with main symptoms of memory deficit, spatial and temporal disorientation, and limited daily activity. The experimental therapeutic intervention was carried out for 22 months with microdoses of a cannabis extract containing cannabinoids. Clinical evaluations using Mini-Mental State Examination and Alzheimer’s Disease Assessment Scale-Cognitive Subscale were performed. Conclusions Here we provide original evidence that cannabinoid microdosing could be effective as an Alzheimer’s disease treatment while preventing major side effects. This is an important step toward dissociating cannabinoids’ health-improving effects from potential narcotic-related limitations.

Abstract Background The review of pharmacotherapy can be conceptualized as a service in which the drugs used by the patient are reviewed to control the risks as well as to improve the results of the drug therapy, detecting, solving, and preventing issues associated with the drug, readjusting the doses and times (schedule) so that the treatment is not incompatible or in duplicity. Methods The aim of the study was to validate an intelligent information system, which was developed to assist the scheduling activity in the pharmacotherapy review. The system used the concept of Genetic Algorithms. To validate the system, hypothetical cases were elaborated considering various aspects of pharmacotherapy such as underdose, overdose, drug interactions and contraindications. These cases were tested in the system and were also analyzed by pharmaceutical experts with clinical and research experience in the pharmacotherapy review process. The degree of agreement between the assessments of the appointments carried out by the pharmaceutical specialists and by the system were measured using the Kappa index with a 95% confidence interval. Results In detecting errors and make propositions, the system was able to identify 80% of errors, with pharmaceutical experts identifying between 20 and 70% of errors. In relation the results of kappa between the cases, the system had 87,3% of concordance, whereas the best pharmaceutical expert had 75,5% of concordance, considering the correct answer. Conclusion It can be concluded that with the methodology used, the investigation met the objectives and confirmed the system is effective for pharmaceutical review process. There are indications that the system can help in the Pharmacotherapy review process, being able to find prescription errors as well as to establish times for the use of medications according to the patient’s routine.

High-throughput screening of 120,000 compounds followed by counter-screening and validation assays reveals candidate antimalarial drugs that kill Plasmodium falciparum sexual and asexual blood stages blocking parasite transmission through mosquito.

IntroductionRecent evidence suggests that the bone marrow (BM) plays a key role in the diffusion of P. falciparum malaria by providing a “niche” for the maturation of the parasite gametocytes, responsible for human-to-mosquito transmission. Suitable humanized in vivo models to study the mechanisms of the interplay between the parasite and the human BM components are still missing.MethodsWe report a novel experimental system based on the infusion of immature P. falciparum gametocytes into immunocompromised mice carrying chimeric ectopic ossicles whose stromal and bone compartments derive from human osteoprogenitor cells.ResultsWe demonstrate that immature gametocytes home within minutes to the ossicles and reach the extravascular regions, where they are retained in contact with different human BM stromal cell types.DiscussionOur model represents a powerful tool to study BM function and the interplay essential for parasite transmission in P. falciparum malaria and can be extended to study other infections in which the human BM plays a role.

One of the most widely cultivated species in the aquaculture sector is the Nile tilapia (Oreochromis niloticus). However, the rising price of fishmeal has a huge impact on its market value. Proteins derived from plants have been used to address the issue. In this study, systematic review and meta-analysis were used to quantitatively analyze the whole-body composition and growth of Nile tilapia fed pea meal. Based on the preferred reporting items for systematic reviews and meta-analysis methods, the literature review and paper selection were completed. Meta-analysis was performed on the data from peer-reviewed studies that met the inclusion screening requirements, namely: tilapia development stage, standard error reporting, feeding period, lack of nutritional supplements, and crude protein levels. The control diet outperformed the plant-based diets according to the result, in which growth and feed utilization characteristics were significantly different (P < 0.05). All except the final weight were significantly better (P < 0.05) in tilapia fed control compared to tilapia fed pea treatment. Additionally, the pooled estimate of whole-body composition of fish fed a diet based on pea meal was not substantially different from that of fish fed a diet based on fishmeal (P > 0.05). Due to large and statistically significant I2 values, the feeding interval, crude protein concentration, fish development stage, and dietary pea meal inclusions were all examined using meta-regression analysis to identify the sources of heterogeneity. Not all co-variates explained the heterogeneity except for the feeding period and crude protein % in some of the parameters. The minimum quantities of dietary pea meal needed for best Nile tilapia performance in terms of growth, feed utilization, and whole-body composition were found to be 4.9, 488.6, and 210.3 g kg−1, respectively.

A Heartland Forgiveness Scale (HFS) é um instrumento que mede a disposição pessoal para o perdão em relação aos outros, a si mesmo e a situações fora do controle de qualquer pessoa. O presente estudo teve como objetivo traduzir e adaptar transculturalmente a HFS para idosos brasileiros. Para tanto, o método de adaptação do instrumento foi divido em etapas: definições conceituais e revisão da literatura; tradução e síntese das traduções; retrotradução (backtraslation); análise de especialistas; estudo piloto e elaboração da versão final do instrumento; primeiras análises psicométricas da versão final. Nas etapas de tradução, retrotradução e análises de especialistas participaram quatro tradutores e duas psicólogas especialistas. Já o estudo piloto contou com a participação de 32 idosos provenientes da região Sul do Brasil, com idades entre 60 e 89 anos, em sua maioria com alto grau de escolaridade. Utilizou-se uma Ficha de dados sócio demográficos e a HFS. A partir desse estudo, ainda que em caráter inicial, pode-se concluir que a HFS está adaptada transculturalmente para a população idosa brasileira. Sendo assim, está apta a ser aplicada em uma amostra maior para que possa ser submetida a análises psicométricas de sua validação. A adaptação de instrumentos de outras culturas nessa temática se faz necessária para realidade brasileira devido a escassez de estudos na área do perdão, em especial com populações de idosos. Futuramente, pode também contribuir para a elaboração de técnicas de intervenção e criação de estratégias voltadas para prevenção e promoção da saúde do idoso.

O artigo inaugura uma investigação sobre um possível processo de gentrificação urbana em município de baixa densidade populacional e com histórico de imigração italiana: a cidade de Nova Veneza | Santa Catarina. O estudo teve por objetivo compreender efeitos iniciais das reiteradas reformas arquitetônicas na Praça Humberto Bortoluzzi – conduzidas pelas investidas do poder público local – nos usos estabelecidos daquele espaço e na transformação urbana do seu entorno, e a estreita relação com o discurso da italianidade. Para dar conta da discussão, partimos da seguinte questão: de que modo as reiteradas reformas da Praça Humberto Bortoluzzi impactam no seu uso e na reconfiguração do seu entorno? Tratou-se de uma pesquisa qualitativa, cujos instrumentos de geração de dados empregados foram observação participante, entrevistas semiestruturadas e conversas informais. A organização textual se apresenta enquanto narrativa, de modo a auxiliar a forma textual, o processo analítico, a descrição espacial e eventuais subjetividades. No campo teórico, as referências utilizadas são de autores provenientes da Antropologia Urbana e do Planejamento Urbano.

Networked microgrids have many advantages for consumers and small energy producers, including higher reliability than non-networked microgrids. However, energy transaction, network interconnection, the intermittent nature of renewable sources, and other problems lead to challenges in the practical implementation of networked microgrids. Despite its favorable use of space, a floating PV system presents challenges that differ from those associated with its land-based counterparts because it is prone to the motion of the surface of the water, resulting in an unpredictable power output. This work presents a hierarchical energy management system (EMS) to address these issues. In level 1 of the EMS, which is for the overall management thereof, a blockchain model is used to manage transactions among microgrids. A grid synchronization algorithm is implemented in level 2 of the EMS, which manages the interconnection of microgrids. Level 2 is activated when an energy transaction between microgrids is needed. An on-line recurrent neural network (RNN)-based controller for an energy storage system (ESS), which is designed specifically to mitigate the problem caused by a floating PV platform, is deployed in level 3 as a local controller. The results of a hardware-in-the-loop (HIL) simulation demonstrate that the EMS can properly coordinate the levels in the hierarchical scheme to interconnect and provide power support between the microgrids. Real-time simulation results show that the ESS controller responds well, proving the viability of the hardware controller. According to these findings, the hierarchical EMS that is proposed in this work can solve the considered problems.

Antimalarial drugs that can block the transmission of Plasmodium gametocytes to mosquito vectors would be highly beneficial for malaria elimination efforts. Identifying transmission-blocking drugs currently relies on evaluation of their activity against gametocyte-producing laboratory parasite strains and would benefit from a testing pipeline with genetically diverse field isolates. The aims of this study were to develop a pipeline to test drugs against P. falciparum gametocyte field isolates and to evaluate the transmission-blocking activity of a set of novel compounds. Two assays were designed so they could identify both the overall transmission-blocking activity of a number of marketed and experimental drugs by direct membrane feeding assays (DMFA), and then also discriminate between those that are active against the gametocytes (gametocyte killing or sterilizing) or those that block development in the mosquito (sporontocidal). These DMFA assays used venous blood samples from naturally infected Plasmodium falciparum gametocyte carriers and locally reared Anopheles gambiae s.s. mosquitoes. Overall transmission-blocking activity was assessed following a 24 hour incubation of compound with gametocyte infected blood (TB-DMFA). Sporontocidal activity was evaluated following addition of compound directly prior to feeding, without incubation (SPORO-DMFA); Gametocyte viability was retained during 24-hour incubation at 37°C when gametocyte infected red blood cells were reconstituted in RPMI/serum. Methylene-blue, MMV693183, DDD107498, atovaquone and P218 showed potent transmission-blocking activity in the TB-DMFA, and both atovaquone and the novel antifolate P218 were potent inhibitors of sporogonic development in the SPORO-DMA. This work establishes a pipeline for the integral use of field isolates to assess the transmission-blocking capacity of antimalarial drugs to block transmission that should be validated in future studies.

IntroductionNile tilapia (Oreochromis niloticus) is one of the most cultured species in the aquaculture industry. However, its market price is affected by the increasing cost of fishmeal. Plant-based proteins are being used to mitigate the problem.MethodologyThis paper comprehensively quantified the growth performance and carcass composition of Nile tilapia fed copra meal via systematic review and meta-analysis. The literature review and paper selection were done based on the preferred reporting items for systematic reviews and meta-analysis method. The data from peer-reviewed papers that qualified from the inclusion screening criteria such as standard error reporting, feeding period, absence of dietary supplements, tilapia development stage, and crude protein levels were subjected to meta-analysis.ResultsResults showed that growth and feed utilization parameters were significantly different (P ≤ 0.05), favoring the control over the plant-based diets. Moreover, the pooled estimate of carcass composition of fish fed copra meal-based diet was not significantly different with that of fish fed fishmeal-based diet (P > 0.05) except for ash content (P < 0.05). The feeding period, crude protein level, fish development stage, and dietary copra inclusions were subjected to meta-regression analysis due to high and statistically significant I2 values to determine the sources of heterogeneity. The residual I2 values showed that these factors did not affect the heterogeneity of the data presented for copra meal. These effects may be attributed to the nutritional quality of raw materials and other abiotic factors like feed preparation.ConclusionNonetheless, data from meta-analysis using model coefficients revealed that the minimum required levels are 594, 617, and 462 g kg−1 of dietary copra meal are needed for optimum growth performance, feed utilization, and carcass composition of Nile tilapia, respectively. The study showed that systematic review and meta-analysis can be a useful tool in optimizing plant-based diets for aquaculture species.

Introduction: Although the efficacy of hydroxyurea (HU) in inhibiting erythrocyte sickling has been well demonstrated, the action of this drug on human neutrophils and the mechanism by which it improves the manifestations of the disease have not been studied thoroughly. We aimed to investigate the cell viability, along with inflammatory and oxidative markers in the neutrophils of sickle cell anemia (SCA) patients and the effects of HU therapy on these cells, by evaluating the dose-responsiveness. Methods: In the present study, 101 patients (45 men and 56 women, aged 18–69 years) with SCA were divided into groups according to the use or not of HU: the SS group (without HU treatment, n = 47) and the SSHU group (under HU treatment, n = 54). The SSHU group was further stratified into subgroups according to the daily dose of the drug that patients already used: SSHU - 0.5 g (n = 19); SSHU - 1 g (n = 26) and SSHU - 1.5–2 g (n = 9). A control group (AA) comprised 50 healthy individuals. Neutrophils isolated from whole blood were analyzed using Trypan Blue, monoiodotyrosine (MTT) and lactate dehydrogenase (LDH) toxicity assays. Myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and concentrations of interleukin 10 (IL-10), tumor necrosis factor alpha (TNF-α) and malonaldehyde (MDA) were also measured. Results: Neutrophils from SCA patients showed membrane fragility and a significant decrease in cell viability when analyzed by Trypan Blue (p

Abstract The use of quantitative qRT-PCR assays for detection and quantification of late gametocyte stages has revealed the high transmission capacity of the human malaria parasite, Plasmodium falciparum. To understand how the parasite adjusts its transmission in response to in-host environmental conditions including antimalarials requires simultaneous quantification of early and late gametocytes. Here, we describe qRT-PCR assays that specifically detect and quantify early-stage P. falciparum gametocytes. The assays are based on expression of known early and late gametocyte genes and were developed using purified stage II and stage V gametocytes and tested in natural and controlled human infections. Genes pfpeg4 and pfg27 are specifically expressed at significant levels in early gametocytes with a limit of quantification of 190 and 390 gametocytes/mL, respectively. In infected volunteers, transcripts of pfpeg4 and pfg27 were detected shortly after the onset of blood stage infection. In natural infections, both early (pfpeg4/pfg27) and late gametocyte transcripts (pfs25) were detected in 71.2% of individuals, only early gametocyte transcripts in 12.6%, and only late gametocyte transcripts in 15.2%. The pfpeg4/pfg27 qRT-PCR assays are sensitive and specific for quantification of circulating sexually committed ring stages/early gametocytes and can be used to increase our understanding of epidemiological processes that modulate P. falciparum transmission.

O presente artigo visa contribuir com uma proposta de recurso didático para auxiliar os conteúdos referentes a flora e as atividades de campo em biogeografia. Desenvolveu-se, a partir disso, a Tabela de Classificação fisionômica das Espécies da Flora, recurso didático direcionado aos estudos e observações das espécies pensados a partir das contribuições realizadas pelo pesquisador Silvio Stefenelli. Entre as contribuições que surgiram com esta pesquisa, destaca-se a importância da utilização de recursos didáticos para se estudar conteúdos biogeográficos nas salas de aula. Entende-se que a utilização do recurso didático aqui proposto venha acrescentar às aulas de geografia, bem como tornar mais prático os estudos da flora, possibilitando ao estudante maior compreensão das espécies e do espaço geográfico.

Water security is one of the pressing challenges in sustainable cities of the future as the demand for freshwater increases along with the environmental stresses caused by climate change. For example, Metro Manila, Philippines, experienced a water crisis brought by increasing demand and lasting impacts of climate change in 2019. In response, the Metropolitan Waterworks and Sewerage System (MWSS), together with its water concessionaires, Maynilad Water Services, Inc. (Maynilad), and Manila Water Company, Inc. (Manila Water), laid out a water infrastructure plan to bridge the water deficit. However, the decision-making process can be iterative and participatory characterized by uncertainty. This study thus proposed decision-making framework to explore the possibility of alternative sustainable water supply networks in Metro Manila through P-graph and analytic hierarchy process (AHP). Alternatives considered were saltwater desalination, rainwater harvesting, and non-revenue water (NRW) recovery. A sustainable network was defined as having low operational (OPEX) and capital expenses (CAPEX), high water security, and low global warming potential (GWP). Relative weights of sustainability, derived through AHP, showed that water security was the most important sustainability criterion among the surveyed experts in water supply construction and operations. P-graph simulations yielded optimal and near-optimal solutions indicating the possibility of not including the controversial water supply infrastructure project (Kaliwa dam) as a prioritized management option. Instead, sustainable supply networks relied on desalination, rainwater harvesting, and NRW recovery are identified as the main components of the optimal water supply network. Overall, the study suggests that better planning and accounting for sustainability is needed to identify and implement projects that do not only serve the short-term needs but also integrate well into the long-term plans.

Starch (ST) is the main determinant of cassava root industrial quality and is routinely quantified using the specific gravity (SG) method, which is simple but lacks precision. This study aimed to estimate the dry matter (DM) and ST content in nine modern cassava genotypes using the SG method and to develop updated empirical equations that relate SG values with DM and ST content. Two field experiments were conducted using a randomized block design with nine genotypes, nine to ten harvest periods, and four replicates. The correlation between root SG and DM was stronger than that between root SG and ST; however, harvest seasonality strongly influenced this relationship. Genotypes varied in their earliness of ST accumulation in the roots, and genotype-specific equations estimated root DM and ST with greater accuracy than equations based on pooled data from all genotypes. Furthermore, the equations from the literature were less accurate in estimating the root DM and ST content than the equations generated in this study. Therefore, these equations can be used by processing industries to improve the predicted cassava root DM and ST content; however, it may be necessary to include the percentage of ST that industries cannot remove from fresh cassava roots during processing.

Apesar da mandioca ser reconhecida pela sua elevada tolerância à seca, a irrigação vem apresentando resultados satisfatórios. Entretanto, poucos estudos vêm sendo desenvolvidos visando a determinação dos efeitos da cobertura do solo, da irrigação e da combinação de ambos no desenvolvimento da cultura. O objetivo deste estudo foi determinar a influência da irrigação e da cobertura plástica do solo, no desempenho agronômico de mandioca de mesa. O plantio foi feito em canteiros, no sistema de fileira dupla com as manivas-sementes implantadas na vertical em espaçamento de 0,6 m entre linhas e 0,80 m entre plantas. Foram aplicados os tratamentos canteiro desnudo sem irrigação, canteiro coberto com plástico de polietileno preto sem irrigação, canteiro desnudo com irrigação e canteiro coberto com plástico de polietileno preto com irrigação. As irrigações foram por aspersão convencional, com base no balanço diário de água do solo na profundidade efetiva do sistema radicular da mandioca nos diferentes estágios de desenvolvimento da cultura. Os caracteres avaliados foram: produtividade de parte aérea, produtividade de raízes, porcentagem de amido nas raízes e tempo para a cocção. A expressão dos caracteres produtividade de raízes, peso da parte aérea e teor de amido nas raízes, foi influenciada pelo manejo da irrigação, pela cobertura do solo e pela interação entre ambos. A utilização individual das tecnologias de irrigação e de cobertura plástica do solo, proporcionaram aumentos de produtividade de raízes de 55% e 13%, respectivamente, e quando foram utilizadas em conjunto a produtividade de raízes aumentou 89%.

Abstract The production and consumption of dulce de leche is one of the most important sweets in the Mercosul Region. However, recent studies have shown consumers’ interest for healthier food and no lactose products, hence creating a demand for this study aims to develop a more nutritional lactose-free dulce de leche with less calories and higher yielding by adding green banana biomass. Five lactose-free dulce de leche formulas have been elaborated in which four were added 5%, 10%, 15% and 20% of green banana biomass and one of them received a standard 0.5% of commercial starch addition. The samples were evaluated regarding its microbiological, physicochemical and sensorial quality. The different lactose-free dulce de leche formulas attended the microbiological standards and demonstrated an increased yield of the green banana biomass concentration. The results indicated that the rise of the green banana biomass concentration has increased moisture and decreased protein, lipids and calories levels. In regards of acceptance, all samples evaluated received grades between 7.23 to 8.72, that is, above 6 which is the minimum value accepted to all the evaluated attributes hence demonstrating the acceptance on behalf of evaluators. All samples presented high ratios of intent to purchase therefore confirming the acceptance results found. It has been observed that the use of green banana biomass aggregated positive characteristics to the product and did not influence the sensorial quality of the sweets, seeing that all of them could improve their yield and thus demonstrating to be a good option to the development of healthier products.

To mitigate the unprecedented health, social, and economic damage of COVID-19, the Philippines is undertaking a nationwide vaccination program to mitigate the effects of the global pandemic. In this study, we interrogated COVID-19 vaccine intent in the country by deploying a nationwide open-access online survey, two months before the rollout of the national vaccination program. The Health Belief Model (HBM) posits that people are likely to adopt disease prevention behaviors and to accept medical interventions like vaccines if there is sufficient motivation and cues to action. A majority of our 7,193 respondents (62.5%) indicated that they were willing to be vaccinated against COVID-19. Moreover, multivariable analysis revealed that HBM constructs were associated with vaccination intention in the Philippines. Perceptions of high susceptibility, high severity, and significant benefits were all good predictors for vaccination intent. We also found that external cues to action were important. Large majorities of our respondents would only receive the COVID-19 vaccines after many others had received it (72.8%) or after politicians had received it (68.2%). Finally, our study revealed that most (21%) were willing to pay an amount of PHP 1,000 (USD20) for the COVID-19 vaccines with an average willing-to-pay amount of PHP1,892 (USD38).