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Your search keyword '"Alam, Md Badrul"' showing total 262 results
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13. Attenuation of melanogenesis by Nymphaea nouchali (Burm. f) flower extract through the regulation of cAMP/CREB/MAPKs/MITF and proteasomal degradation of tyrosinase.

Author

Alam, Md Badrul, Ahmed, Arif, Motin, Md Abdul, Kim, Sunghwan, and Lee, Sang-Han

Subjects
Agaricales, Animals, Cyclic AMP, Cyclic AMP Response Element-Binding Protein, Flowers, Hypopigmentation, Melanins, Mice, Mice, Hairless, Microphthalmia-Associated Transcription Factor, Mitogen-Activated Protein Kinases, Monophenol Monooxygenase, Nymphaea, Phosphorylation, Plant Extracts, Polyphenols, Proteasome Endopeptidase Complex, Proteolysis, Signal Transduction, Ultraviolet Rays, Hairless
Abstract

Medicinal plants have been used to treat diseases from time immemorial. We aimed to examine the efficacy of the ethyl acetate fraction of Nymphaea nouchali flower extract (NNFE) against melanogenesis process, and the underlying mechanisms in vitro and in vivo. Paper spray ionisation mass spectroscopy and (+) mode electrospray ionisation revealed the presence of seven flavonoids, two spermidine alkaloids, 3,4,8,9,10-pentahydroxy-dibenzo[b,d]pyran-6-one, and shoyuflavone C in NNFE. NNFE (100 µg/mL) significantly inhibited the monophenolase and diphenolase activities of mushroom tyrosinase at 94.90 ± 0.003% and 93.034 ± 0.003%, respectively. NNFE significantly suppressed cellular tyrosinase activity and melanin synthesis in vitro in melan-a cells and in vivo in HRM2 hairless mice. Furthermore, NNFE inhibited tyrosinase (TYR), tyrosinase-related protein (TYRP)-1, TYRP-2, and microphthalmia-associated transcription factor (MITF) expression, thereby blocking melanin synthesis. In particular, NNFE suppressed cAMP production with subsequent downregulation of CREB phosphorylation. Additionally, it stimulated MAP kinase phosphorylation (p38, JNK, and ERK1/2) and the proteasomal debasement pathway, leading to degradation of tyrosinase and MITF and the suppression of melanin production. Moreover, selective inhibitors of ERK1/2, JNK, and p38 attenuated NNFE inhibitory effects on melanogenesis, and MG-132 (a proteasome inhibitor) prevented the NNFE-induced decline in tyrosinase protein levels. In conclusion, these findings indicate that NNFE is a potential therapy for hyperpigmentation.

Published
2018

16. Glutamic-Alanine Rich Glycoprotein from Undaria pinnatifida : A Promising Natural Anti-Inflammatory Agent.

Author

Rahman, Md Saifur, Alam, Md Badrul, Naznin, Marufa, Madina, Mst Hur, and Rafiquzzaman, S. M.

Abstract

This study aimed to assess the anti-inflammatory properties of a bioactive glutamic-alanine rich glycoprotein (GP) derived from Undaria pinnatifida on both LPS-stimulated RAW264.7 cells, peritoneal macrophages, and mouse models of carrageenan- and xylene-induced inflammation, investigating the underlying molecular mechanisms. In both in-vitro and in-vivo settings, GP was found to reduce the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) while also inhibiting the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in response to lipopolysaccharide (LPS) stimulation. GP treatment significantly impeded the nuclear translocation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway by blocking the phosphorylation of IKKα and IκBα, leading to a reduction in proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). Additionally, GP effectively inhibited the activation of mitogen-activated protein kinases (MAPKs), with specific inhibitors of p38 and extra-cellular signal regulated kinase (ERK) enhancing GP's anti-inflammatory efficacy. Notably, GP administration at 10 mg/kg/day (p.o.) markedly reduced carrageenan-induced paw inflammation and xylene-induced ear edema by preventing the infiltration of inflammatory cells into targeted tissues. GP treatment also downregulated key inflammatory markers, including iNOS, COX-2, IκBα, and NF-κB, by suppressing the phosphorylation of p38 and ERK, thereby improving the inflammatory index in both carrageenan- and xylene-induced mouse models. These findings suggest that marine resources, particularly seaweeds like U. pinnatifida, could serve as valuable sources of natural anti-inflammatory proteins for the effective treatment of inflammation and related conditions. [ABSTRACT FROM AUTHOR]

Published
2024
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27. Health-seeking behaviour of stroke patients in a rural area of Bangladesh

Author

Alam, Md Badrul, Saha, Uttam Kumar, Mashreky, Saidur Rahman, Hussain, AHM Enayet, Haque, Md Atiqul, Rahman, AKM Fazlur, Dalal, Koustuv, Mohammad, Quazi Deen, Alam, Md Badrul, Saha, Uttam Kumar, Mashreky, Saidur Rahman, Hussain, AHM Enayet, Haque, Md Atiqul, Rahman, AKM Fazlur, Dalal, Koustuv, and Mohammad, Quazi Deen

Abstract

Background: Improper health-seeking behaviours (HSB) have been correlated with detrimental health outcomes, elevated rates of illness and mortality. The study aimed to investigate how stroke patients in a rural community of Bangladesh seek health care. Methods: A cross-sectional survey was conducted in the Raiganj sub-district of Sirajganj district from January to June 2016, using a validated screening tool to identify stroke patients at the household level. Neurologists confirmed the diagnosis after examining all suspected cases. Out of the 419 suspected cases identified during the screening process, 186 cases were officially reported after undergoing a confirmed diagnosis. Information on health-seeking behaviour was collected through face-to-face interviews with patients or their attendants. Results: After experiencing a stroke, approximately 35% of patients received treatment from unregistered care providers and over 40% received treatment outside of a hospital setting. Males were significantly more likely than females to receive treatment from registered physicians or hospitals (P<.05 and P<.01). A significantly higher proportion of educated individuals sought healthcare from registered physicians or hospitals (P<.05). Although better health-seeking behaviour was observed among higher-income groups, the findings were not statistically significant. Around 67% of patients were found to be hypertensive, with about one-third of them not taking any medication for their elevated blood pressure. Approximately 37% of patients had elevated blood glucose levels but only 22% were taking medication. Conclusion: A notable proportion of stroke patients in rural Bangladesh sought treatment from unqualified service providers. Health-seeking behaviour was associated with factors such as gender, education, and economic condition.

Published
2023
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36. On-farm performance of genetically-improved rohu (Labeo rohita) in Bangladesh

Author

Hamilton, Matthew G., primary, Yeasin, Mohammed, additional, Alam, Md. Badrul, additional, Ali, Md. Rayhan, additional, Fakhruddin, Md., additional, Islam, Md. Mazharul, additional, Barman, Benoy K., additional, Shikuku, Kelvin Mashisia, additional, Shelley, Colin C., additional, Rossignoli, Cristiano M., additional, and Benzie, John A. H., additional

Published
2022
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47. Understanding the Biodegradation Pathways of Azo Dyes by Immobilized White-Rot Fungus Trametes Hirsuta D7 Using Uplc-Ft-Icr-Ms Supported by in Silico Simulations and Toxicity Assessment

Author

Alam, Rafiqul, primary, Mahmood, Raisul Awal, additional, Islam, Syful, additional, Ardiati, Fenny Clara, additional, Solihat, Nissa Nurfajrin, additional, Alam, Md Badrul, additional, Lee, Sang Han, additional, Yanto, Dede Heri Yuli, additional, and Kim, Sunghwan, additional

Published
2022
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