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This experiment was conducted in 16/3 / 2016 to 21/ 4 / 2016. The location was doing in research Hall at animal Resources department , college of Agricultural university of kufa, associated with the Humanisistic Scientific College, Department of Medical Laborites Sciences / laboratory of Histology and Anatomy. The design was RCBD with 2 replication, Broiler bird from Ross type (380) one day age were used and divided into 5 group. Each group contain 26 bird, the treatment were 5 as the following : 1- Group (T1) control treatment in which given normal diet without any amount of Pleurouts ostreatus mixed with Barley. 2- Group (T2) which were given 25%. Of mixed Barely/ in chick diet daily. 3- Group (T3) which were given 50% of mixed Barely/ in chick diet daily. 4- Group (T4) which were given 75% of mixed Barely in chick diet daily. 5-Group (T5) which were given 100% of mixed Barely in chick diet daily. The treatment started at the beginning of one day for 5 week at the end of experiment. Take sample of blood from each bird to analysis at end of 5 week to known the level of immunity (H.I. test) show that the group T5 gave high result in compression to other treatment at level P<0.05 (691). The weight of Buras fabricius is differ, it show high weight at group T4 =2.97 g. Group T1=2.95g, T2=2.88 the less weight at T3=2.13, T5=2.17. The histological change of Bursa fabricius gland is different between the control group T1 and other group, depended upon the amount of mixed Barley which take it. The group T4 show the histological change was more effect and activity at the other groups, which give the chickens 75% from mixed barley. [ABSTRACT FROM AUTHOR]