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18 results on '"Al-Jawabreh, Hanan"'

9. Additional file 1 of Concurrent molecular characterization of sand flies and Leishmania parasites by amplicon-based next-generation sequencing

Author

Nasereddin, Abedelmajeed, Ereqat, Suheir, Al-Jawabreh, Amer, Taradeh, Mohamad, Abbasi, Ibrahim, Al-Jawabreh, Hanan, Sawalha, Samer, and Abdeen, Ziad

Abstract

Additional file 1: Table S1. Reference DNA samples for Leishmania and sand fly species used in the study. Table S2. Sand flies and Leishmania GenBank accession numbers used in this study for primer designing. Table S3. The virtual probe sequences used for detection and identification of sand flies and Leishmania parasites.

Published
2022
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15. Serological and molecular survey of Leishmania parasites in apparently healthy dogs in the West Bank, Palestine

Author

Hamarsheh Omar, Nasereddin Abedalmajeed, Damaj Safa, Sawalha SamIr, Al-Jawabreh Hanan, Azmi Kifaya, Amro Ahmad, Ereqat Suheir, Abdeen Ziad, and Al-Jawabreh Amer

Subjects
Serological and molecular diagnosis, Leishmania, Domestic dogs, West bank, Palestine, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Canine visceral leishmaniasis (CVL) is caused by Leishmania infantum in all Mediterranean countries. The Leishmania parasite is transmitted by the bite of a corresponding sand fly vector and primarily maintained in nature by wild and domestic reservoirs, including dogs, foxes and jackals. Infected dogs are the primary reservoir host in endemic regions and are the most significant risk disposing humans to infection. The present study aimed at assessing the prevalence of infection with Leishmania and identification of Leishmania infantum in domestic dogs in the West Bank, Palestine. Methods The infection rate among domestic dogs collected from seven districts in the Palestinian West Bank was investigated by examination of parasites in culture from the buffy coat using serological and molecular methods; based on ELISA, internal transcribed spacer 1 (ITS1) and cysteine protease (CPB) PCR. Results Out of 215 dogs examined for Leishmania, 36 (16.7%) were positive in at least one method. Twenty three animals (11.5%) were positive for Leishmania DNA, whereas, ELISA and culture revealed 16 (7.5%), and 4 (1.5%) respectively. CPB-PCR on one of three culture-positive isolates revealed Leishmania infantum as the causative agent for Leishmania infection in dogs. Conclusions Our study showed that canine leishmania infection is prevalent with varying degrees in all the seven studied districts in Palestine despite the absence of human VL cases in 4 of these districts. The causative agent was confirmed to be Leishmania infantum.

Published
2012
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