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We compared the results of routine blood tests for 102 blood donors’ samples and 100 patients’ samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs. K3EDTA), its application (liquid vs. spray- dried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate (Olympus® PK 7200) and gel column methods (Ortho ID-Micro Typing System™/Gel Test™). Additional studies on blood donors’ samples included time delayed antigen testing and antibody identification and half-draw/half- evacuated collections. Also, we compared the results of routine ABO/D testing and antibody screening for 50 patients’ samples collected in spray-dried K2EDTA and spray-dried K3EDTA and for an additional 50 patients’ samples collected in spray-dried K2EDTA tubes from two different manufacturers. All patients’ samples were tested in parallel by solid phase/microplate method (Immucor® ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors’ and patients’ samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.

A woman's candidacy for Rh immune globulin depends on whether her blood type is Rh-positive (D antigen-positive) or Rh-negative (D antigen-negative). New molecular blood-typing methods have identified variant D antigens, which may be reported as Rh-positive or Rh-negative depending on the laboratory method. We describe a case illustrating the effect of the new laboratory methods on a woman's candidacy for Rh immune globulin and present recommendations for interpreting the new test results.A 40-year-old woman presented for management of her third pregnancy. During her first pregnancy, she was typed as Rh-positive ("D") and did not receive Rh immune globulin. During her second pregnancy, she was typed as Rh-negative, in accordance with revised Rh-typing procedures. Anti-D antibody was detected. During her third pregnancy, she was genotyped as a partial D antigen, which was reported as Rh-negative.Revisions in laboratory procedures for Rh typing may present as a change in the Rh blood type of pregnant women-and as a change in their eligibility for Rh immune globulin.

BACKGROUND: We evaluated the potential for radiofrequency (RF) transponder microchips to standardize and document key steps in the blood collection and transfusion process. STUDY DESIGN AND METHODS: Using the blood center's standard operating procedures for blood collections, we programmed a laptop computer and 10 multiwrite 256-byte RF microchips to prompt operators to enter data for key steps in blood collection. Before collections, RF microchips were attached to blood collection sets at the blood center. In parallel with actual collections, we added data to the microchips with the computer and a hand-held scanner-programmer. After labeling, we shipped the RF microchip-tagged blood units to the hospital where unit-related data (whole blood number, ABO and Rh, expiration date, special laboratory test results) were uploaded from the RF microchip to the transfusion service's information system. The microchip was subsequently used as a cross-match label for blood unit–recipient matching. RESULTS: Data were successfully uploaded to the RF microchip at key steps during blood collections. Software programs in the laptop computer and hand-held scanner-programmer successfully prompted operators to enter key data. At any stage in a blood collection, authorized operators were able to review electronic records of prior steps using the laptop computer or by scanning the microchip attached to the blood bag. Unit-related data were successfully transferred to the hospital transfusion service through the RF microchip. These data were successfully incorporated in the RF microchip cross-match label, which was used to confirm recipient–blood unit matching at the bedside. CONCLUSION: RF microchips can collect key data during blood collections, facilitate information transfer from the blood center to the hospital, and confirm recipient–blood unit matching at the bedside before transfusions.

We compared the results of routine blood tests for 102 blood donors' samples and 100 patients' samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs.K3EDTA), its application (liquid vs. spray-dried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate (Olympus(R) PK 7200) and gel column methods (Ortho ID-Micro Typing System/Gel Test). Additional studies on blood donors' samples included time delayed antigen testing and antibody identification and half-draw/half-evacuated collections. Also, we compared the results of routine ABO/D testing and antibody screening for 50 patients' samples collected in spray-dried K2EDTA and spray-dried K3EDTA and for an additional 50 patients' samples collected in spray-dried K2EDTA tubes from two different manufacturers. All patients' samples were tested in parallel by solid phase/microplate method (Immucor ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors' and patients' samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.