Search

Your search keyword '"Akutsu-Shigeno, Yukie"' showing total 12 results
Start Over Author "Akutsu-Shigeno, Yukie"
12 results on '"Akutsu-Shigeno, Yukie"'

6. Cloning and sequencing of a poly(DL-lactic acid) depolymerase gene from Paenibacillus amylolyticus strain TB-13 and its functional expression in Escherichia coli

Author

Akutsu-Shigeno, Yukie, Teeraphatpornchai, Teerawat, Teamtisong, Kamonluck, Nomura, Nobuhiko, Uchiyama, Hiroo, Nakahara, Tadaatsu, and Nakajima-Kambe, Toshiaki

Subjects
Microbiology -- Research, Microbiology -- Environmental aspects, Cloning -- Genetic aspects, Lactic acid -- Physiological aspects, Polymers -- Physiological aspects, Gene expression -- Physiological aspects, Escherichia coli -- Genetic aspects, Escherichia coli -- Physiological aspects, Biodegradation -- Analysis, Biological sciences
Abstract

Research has been conducted on gene encoding poly(DL-lactic acid) depolymerase from Paenibacillus amylolyticus strain TB-13. The authors report the cloning and overexpression of this gene, and demonstrate that purified recombinant poly(DL-lactic acid) depolymerase tends to degrade different biodegradable polyesters.

Published
2003

12. Matrix metalloproteinase-2 inhibition and Zn2+-chelating activities of pyoverdine-type siderophores

Author

Shinozaki-Tajiri, Yukiko, Akutsu-Shigeno, Yukie, Nakajima-Kambe, Toshiaki, Inomata, Shinji, Nomura, Nobuhiko, and Uchiyama, Hiroo

Subjects
*METALLOPROTEINASES, *CHROMATOGRAPHIC analysis, *PSEUDOMONAS, *CHELATES, *METAL ions
Abstract

Pyoverdine-type siderophores from fluorescent Pseudomonas species were purified by Zn2+-chelate chromatography, and their matrix metalloproteinase-2 (MMP-2) inhibition and metal-ion-chelating activities were studied. Structurally different pyoverdines showed different MMP-2 inhibition activities, and the inhibition activity was correlated with Zn2+-chelating activity. The IC50 value of a pyoverdine (P113A1-2, MW 1187) for MMP-2 was 0.27 μg/ml (0.23 μM). [Copyright &y& Elsevier]

Published
2004
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results