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In this study, we assessed the co-occurrence influence of Hyptis suaveolens and Urena lobata on native plant species and soil properties in a guinea savanna vegetation in Nigeria. We sampled 120 plots of 10 x 10 m² with 30 plots each in sites invaded by H. suaveolens, U. lobata, mixed site and in sites with none of the species (control). A sparse partial least square discriminant analysis was used to assess the effect of invasive plant treatments on the plant diversity and soil properties, whereas the relationships between the soil properties, plant diversity and invasive species treatments were assessed using the canonical correspondence analysis. The indices of diversity of the control were significantly higher than all the other treatments (p < 0.001) with the mixed site having the lowest. There were significant differences in phosphorus, calcium, aluminium, soil alkalinity and diversity indices among the treatments. The results also indicated that the diversity indices and some soil properties were negatively associated with the mixed site. The negative impacts on the native diversity and change in the soil properties caused by the co-invasion of these two plants are more additive than non-additive. Therefore, priority should be placed on the management of co-invaded sites. [ABSTRACT FROM AUTHOR]