19 results on '"Akitomo K"'
Search Results
2. Downregulation of human CD46 by adenovirus serotype 35 vectors
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Sakurai, F, Akitomo, K, Kawabata, K, Hayakawa, T, and Mizuguchi, H
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- 2007
- Full Text
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3. Instabilities of the tidally induced bottom boundary layer in the rotating frame and their mixing effect
- Author
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Sakamoto, K. and Akitomo, K.
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- 2006
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4. Therapeutic response and prognostic factors of 14 dogs undergoing transcatheter arterial embolization for hepatocellular masses: A retrospective study
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Yuta Kawamura, Hiroki Itou, Akitomo Kida, Hiroki Sunakawa, Moe Suzuki, and Kenji Kawamura
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canine ,hemoabdomen ,interventional oncology ,median survival time ,tumor reduction rate ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background Information regarding the therapeutic effect and outcome of transcatheter arterial embolization (TAE) for hepatic masses is limited in veterinary medicine. Hypothesis/Objectives To analyze the therapeutic response, outcome (overall survival), and their predictors in dogs that underwent TAE for primary hepatocellular masses. We hypothesized that larger pre‐TAE tumors would be associated with worse outcomes. Animals Fourteen client‐owned dogs. Methods Retrospective study. Medical records between 1 September 2016 and 30 April 2022 were reviewed to identify dogs treated with TAE for hepatic masses diagnosed as hepatocellular origin by cytological or histopathological examination. Computed tomography images were compared before and after TAE. The univariate Cox proportional hazards test was performed to assess the associations between variables and survival. Univariate linear regression analysis was performed to assess the associations between variables and the tumor reduction percentage: ([post‐TAE volume − pre‐TAE volume]/pre‐TAE volume) × 100. Results The median survival time was 419 days (95% confidence interval, 82‐474). History of intra‐abdominal hemorrhage (P = .03) and pre‐TAE tumor volume/body weight (P = .009) were significantly associated with overall survival. The mean reduction percentage was −51% ± 40%. Pre‐TAE tumor volume/body weight ratio (cm3/kg; P = .02, correlation coefficient = 0.704) was significantly correlated with the volume reduction percentage. Conclusions History of intra‐abdominal hemorrhage and large pre‐TAE tumor volume/body weight ratio could be predictive factors for adverse outcomes after TAE. Pre‐TAE tumor volume/body weight ratio could be a predictive factor for therapeutic effect.
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- 2023
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5. Microwave ablation for the control of bleeding from disintegrated mammary tumours in two dogs
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Yuta Kawamura, Hiroki Itou, Akitomo Kida, Hiroki Sunakawa, and Kenji Kawamura
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breeding ,cancer ,dog ,haemostasis ,mammary gland ,Veterinary medicine ,SF600-1100 - Abstract
Abstract A 16‐year‐old intact female Miniature Dachshund (dog 1) and a 13‐year‐old intact female American Cocker Spaniel (dog 2) presented with a chief complaint of bleeding from a mammary gland tumour ulceration. Dog 1 was transferred to hospital from a local hospital in a haemorrhagic shock state with uncontrolled continuous bleeding. Thoracic radiographs revealed multiple nodular shadows suspected to be pulmonary metastasis. Dog 2 presented with intermittent bleeding from a mass lesion in the right fifth mammary gland. Due to high anaesthetic risk secondary to severe mitral valve insufficiency (ASA status III), the owner declined surgical excision of the tumour. Therefore, microwave ablation (MWA) under local anaesthesia was chosen in order to achieve adequate haemostasis. Both dogs received local anaesthesia around the bleeding mass lesion, and the disintegrated site was microwave‐ablated; dog 1 underwent MWA after blood transfusion to improve the haemorrhagic shock. The ablation site was protected using a non‐adhesive dressing. Scarring of the ulcerated site led to complete haemostasis in both cases. Dog 1 underwent tumorectomy on the 31st hospital day to prevent rebleeding; histopathology results were consistent with mammary adenocarcinoma with the ablation site covered by a capsule structure. To the authors’ knowledge, this is the first case report describing the use of MWA to stop bleeding from mammary tumours in veterinary medicine. MWA is a feasible and potentially effective palliative treatment modality to stop bleeding from disintegrated mammary tumours in dogs under local anaesthesia.
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- 2023
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6. Successful excision of an inflammatory endobronchial polyp using biopsy forceps with improvement in FEV1 in a patient with allergic bronchopulmonary aspergillosis: A case report
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Fumihiro Kashizaki, Kenji Konishi, Chihiro Yamada, Shunsuke Okazaki, Hao Chen, Atsushi Miyasaka, Nanami Tsuchiya, Akitomo Kikuchi, Kentaro Yumoto, Yui Kojima, Hiroyuki Osawa, Harumi Koizumi, Kenichi Takahashi, and Takeshi Kaneko
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Allergic bronchopulmonary aspergillosis ,Inflammatory endobronchial polyps ,Biologic ,Biopsy forceps ,Flexible bronchoscopy ,Pulmonary function test ,Diseases of the respiratory system ,RC705-779 - Abstract
Inflammatory endobronchial polyps (IEPs) are rare, benign bronchial tumors posing diagnostic and therapeutic challenges owing to limited data. A 55-year-old man, receiving treatment for allergic bronchopulmonary aspergillosis, presented with a one-week history of fever and purulent sputum. Diagnosed with pneumonia, he received antimicrobial treatment. However, because of persistent symptoms, an endobronchial tumor was suspected on computed tomography. IEP was confirmed through flexible bronchoscopy with forceps biopsy, and polyp removal improved symptoms, lung function, and imaging.
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- 2024
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7. The eDNA-Container App: A Simple-to-Use Cross-Platform Package for the Reproducible Analysis of eDNA Sequencing Data
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David Wheeler, Lillian Brancalion, Akitomo Kawasaki, and Meaghan L. Rourke
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environmental DNA ,QIIME2 ,Docker ,GUI ,biosecurity ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
The analysis of environmental DNA (eDNA) is a powerful and non-invasive method for monitoring the presence of species in ecosystems. However, ecologists and laboratory staff can find it challenging to use eDNA analysis software effectively due to the unfamiliar command-line interfaces used by many of these packages. Therefore, we developed the eDNA-container app, a free and open-source software package that provides a simple user-friendly interface for eDNA analysis. The application is based on the popular QIIME2 library and is distributed as a Docker image. The use of Docker makes it compatible with a wide range of operating systems and facilitates the reproducible analysis of data across different laboratories. The application includes a point-and-click user interface for selecting sequencing files, configuring parameters, and accessing the results. Key pipeline outputs, such as sequence quality plots, denoising, and ASV generation statistics, are automatically included in a PDF report. This open-source and freely available analysis package should be a valuable tool for scientists using eDNA in biodiversity and biosecurity applications.
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- 2024
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8. Adenovirus serotype 35 vector-mediated transduction following direct administration into organs of nonhuman primates
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Hiroaki Shibata, Takao Hayakawa, Shinichiro Nakamura, Keiji Terao, Akitomo K, Kenji Kawabata, Fuminori Sakurai, and Hiroyuki Mizuguchi
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Administration, Topical ,Genetic enhancement ,Transgene ,Genetic Vectors ,Gene delivery ,Biology ,medicine.disease_cause ,Virus ,Adenoviridae ,Injections ,Membrane Cofactor Protein ,Transduction (genetics) ,Transduction, Genetic ,Genetics ,medicine ,Animals ,Tissue Distribution ,Transgenes ,Receptor ,Molecular Biology ,Microglia ,beta-Galactosidase ,Virology ,Molecular biology ,Macaca fascicularis ,medicine.anatomical_structure ,Gene Expression Regulation ,Molecular Medicine - Abstract
Adenovirus (Ad) serotype 35 (Ad35) vectors have attracted remarkable attention as alternatives to conventional Ad serotype 5 (Ad5) vectors. In a previous study, we showed that intravenously administered Ad35 vectors exhibited a safer profile than Ad5 vectors in cynomolgus monkeys, which ubiquitously express CD46, an Ad35 receptor, in a pattern similar to that in humans. However, the Ad35 vectors poorly transduced the organs. In this study, we examined the transduction properties of Ad35 vectors after local administration into organs of cynomolgus monkeys. The vectors transduced different types of cells depending on the organ. Hepatocytes and microglia were mainly transduced after the vectors were injected into the liver and cerebrum, respectively. Injection of the vectors into the femoral muscle resulted in the transduction of cells that appeared to be fibroblasts and/or macrophages. Conjunctival epithelial cells showed transgene expression following infusion into the vitreous body of the eyeball. Transgene expression was limited to areas around the injection points in most of the organs. In contrast, Ad35 vector-mediated transgene expression was not detected in any of the organs not injected with Ad35 vectors. These results suggest that Ad35 vectors are suitable for gene delivery by direct administration to organs.
- Published
- 2008
9. Downregulation of human CD46 by adenovirus serotype 35 vectors
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Kenji Kawabata, Fuminori Sakurai, Takao Hayakawa, Akitomo K, and Hiroyuki Mizuguchi
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Adenoviridae Infections ,viruses ,Blotting, Western ,Genetic Vectors ,Green Fluorescent Proteins ,Down-Regulation ,Biology ,medicine.disease_cause ,Virus ,Membrane Cofactor Protein ,Measles virus ,Downregulation and upregulation ,Cell Line, Tumor ,Genetics ,medicine ,Humans ,Vector (molecular biology) ,Luciferases ,Molecular Biology ,Reverse Transcriptase Polymerase Chain Reaction ,CD46 ,Adenoviruses, Human ,Genetic Therapy ,Flow Cytometry ,biology.organism_classification ,Virology ,Molecular biology ,female genital diseases and pregnancy complications ,Mastadenovirus ,Adenoviridae ,Cell culture ,Molecular Medicine ,Genetic Engineering - Abstract
Human CD46 (membrane cofactor protein), which serves as a receptor for a variety of pathogens, including strains of measles virus, human herpesvirus type 6 and Neisseria, is rapidly downregulated from the cell surface following infection by these pathogens. Here, we report that replication-incompetent adenovirus (Ad) serotype 35 (Ad35) vectors, which belong to subgroup B and recognize human CD46 as a receptor, downregulate CD46 following infection. A decline in the surface expression of CD46 in human peripheral blood mononuclear cells was detectable 6 h after infection, and reached maximum (72%) 12 h after infection. Ad35 vector-induced downregulation of surface CD46 levels gradually recovered after the removal of Ad35 vectors, however, complete recovery of CD46 expression was not observed even at 96 h after removal. The surface expression of CD46 was also reduced after incubation with fiber-substituted Ad serotype 5 (Ad5) vectors bearing Ad35 fiber proteins, ultraviolet-irradiated Ad35, vectors and recombinant Ad35 fiber knob proteins; in contrast, conventional Ad5 vectors did not induce surface CD46 downregulation, suggesting that the fiber knob protein of Ad35 plays a crucial role in the downregulation of surface CD46 density. These results have important implications for gene therapy using CD46-utilizing Ad vectors and for the pathogenesis of Ads that interact with CD46.
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- 2007
10. Case Report: Transcatheter Arterial Embolization for the Initial Management of Intra-Abdominal Hemorrhage From a Hepatic Tumor in a Cat
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Yuta Kawamura, Hiroki Itou, Akitomo Kida, Hiroki Sunkawa, and Kenji Kawamura
- Subjects
transcatheter arterial embolization ,intra-abdominal hemorrhage ,hemostasis ,hepatic mass ,feline ,Veterinary medicine ,SF600-1100 - Abstract
An 8-year-old Ragdoll cat was admitted to our hospital after its owner noticed sudden lethargy. Abdominal ultrasonography showed a large amount of blood in the abdominal cavity, and the cat was diagnosed as having hemorrhagic shock caused by the rupture of an intra-abdominal mass. Blood transfusion was performed on the 1st day of hospitalization. On the 2nd day, contrast-enhanced computed tomography (CT) was performed, and hemorrhage from a mass originating in the caudate lobe of the liver was noted. Transcatheter arterial embolization (TAE) was performed to stop the bleeding from the mass using Gelpart to embolize the feeding artery. The following day, fever and elevation of liver enzyme levels were observed, but these subsided within a few days. At discharge 5 days after TAE, no fluid was found in the peritoneal cavity, and no further intra-abdominal bleeding occurred. Sixty-six days after TAE, we were able to perform resection surgery with the cat in good condition. A partial response was observed on CT performed before surgery. Histopathology revealed cholangiocellular adenoma. The cat was doing well as of postoperative day 549. This case indicates that TAE may be effective for initial hemostasis and stabilization of conditions in animals with tumor-induced hemorrhage.
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- 2021
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11. Two types of thermobaric deep convection possible in the Greenland Sea
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Akitomo, K., primary
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- 2011
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12. Baroclinic instability and submesoscale eddy formation in weakly stratified oceans under cooling
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Akitomo, K., primary
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- 2010
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13. Adenovirus serotype 35 vector-mediated transduction following direct administration into organs of nonhuman primates
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Sakurai, F, primary, Nakamura, S-i, additional, Akitomo, K, additional, Shibata, H, additional, Terao, K, additional, Kawabata, K, additional, Hayakawa, T, additional, and Mizuguchi, H, additional
- Published
- 2008
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14. A sterile hydroponic system for characterising root exudates from specific root types and whole-root systems of large crop plants
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Akitomo Kawasaki, Shoko Okada, Chunyan Zhang, Emmanuel Delhaize, Ulrike Mathesius, Alan E. Richardson, Michelle Watt, Matthew Gilliham, and Peter R. Ryan
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Hydroponic ,Root exudates ,Organic anion ,Malate ,Aluminium-tolerance ,Wheat ,Plant culture ,SB1-1110 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exudates can be bound by minerals or consumed by microorganisms. Exudates are easier to measure with hydroponically-grown plants but, even here, simple compounds such as sugars and organic acids can be rapidly assimilated by microorganisms. Sterile hydroponic systems avoid this shortcoming but it is very difficult to maintain sterility for long periods especially for larger crop species. As a consequence, studies often use small model species such as Arabidopsis to measure exudates or use seedlings of crop plants which only have immature roots systems. Results We developed a simple hydroponic system for cultivating large crop plants in sterile conditions for more than 30 days. Using this system wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) plants were grown in sterile conditions for 30 days by which time they had reached the six-leaf stage and developed mature root systems with seminal, nodal and lateral roots. To demonstrate the utility of this system we characterized the aluminium-activated exudation of malate from the major types of wheat roots for the first time. We found that all root types measured released malate but the amounts were two-fold greater from the seminal and nodal axile roots compared with the lateral roots. Additionally, we showed that this sterile growth system could be used to collect exudates from intact whole root systems of barley. Conclusions We developed a simple hydroponic system that enables cereal plants to be grown in sterile conditions for longer periods than previously recorded. Using this system we measured, for the first time, the aluminium-activated efflux of malate from the major types of wheat roots. We showed the system can also be used for collecting exudates from intact root systems of 30-day-old barley plants. This hydroponic system can be modified for various purposes. Importantly it enables the study of exudates from crop species with mature root systems.
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- 2018
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15. Restriction of convective depth in the Weddell Sea
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Akitomo, K., primary
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- 2007
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16. The Presence of Plant-Associated Bacteria Alters Responses to N-acyl Homoserine Lactone Quorum Sensing Signals that Modulate Nodulation in Medicago Truncatula
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Debora F. Veliz-Vallejos, Akitomo Kawasaki, and Ulrike Mathesius
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acyl-homoserine lactones ,legume ,microbiome ,nodulation ,rhizobia ,quorum sensing ,Botany ,QK1-989 - Abstract
Bacteria use quorum sensing signaling for cell-to-cell communication, which is also important for their interactions with plant hosts. Quorum sensing via N-acyl-homoserine lactones (AHLs) is important for successful symbioses between legumes and nitrogen-fixing rhizobia. Previous studies have shown that plant hosts can recognize and respond to AHLs. Here, we tested whether the response of the model legume Medicago truncatula to AHLs from its symbiont and other bacteria could be modulated by the abundance and composition of plant-associated microbial communities. Temporary antibiotic treatment of the seeds removed the majority of bacterial taxa associated with M. truncatula roots and significantly altered the effect of AHLs on nodule numbers, but lateral root density, biomass, and root length responses were much less affected. The AHL 3-oxo-C14-HSL (homoserine lactone) specifically increased nodule numbers but only after the treatment of seeds with antibiotics. This increase was associated with increased expression of the early nodulation genes RIP1 and ENOD11 at 24 h after infection. A 454 pyrosequencing analysis of the plant-associated bacteria showed that antibiotic treatment had the biggest effect on bacterial community composition. However, we also found distinct effects of 3-oxo-C14-HSL on the abundance of specific bacterial taxa. Our results revealed a complex interaction between plants and their associated microbiome that could modify plant responses to AHLs.
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- 2020
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17. Microbiome and Exudates of the Root and Rhizosphere of Brachypodium distachyon, a Model for Wheat.
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Akitomo Kawasaki, Suzanne Donn, Peter R Ryan, Ulrike Mathesius, Rosangela Devilla, Amanda Jones, and Michelle Watt
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Medicine ,Science - Abstract
The rhizosphere microbiome is regulated by plant genotype, root exudates and environment. There is substantial interest in breeding and managing crops that host root microbial communities that increase productivity. The eudicot model species Arabidopsis has been used to investigate these processes, however a model for monocotyledons is also required. We characterized the rhizosphere microbiome and root exudates of Brachypodium distachyon, to develop it as a rhizosphere model for cereal species like wheat. The Brachypodium rhizosphere microbial community was dominated by Burkholderiales. However, these communities were also dependent on how tightly they were bound to roots, the root type they were associated with (nodal or seminal roots), and their location along the roots. Moreover, the functional gene categories detected in microorganisms isolated from around root tips differed from those isolated from bases of roots. The Brachypodium rhizosphere microbiota and root exudate profiles were similar to those reported for wheat rhizospheres, and different to Arabidopsis. The differences in root system development and cell wall chemistry between monocotyledons and eudicots may also influence the microorganism composition of these major plant types. Brachypodium is a promising model for investigating the microbiome of wheat.
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- 2016
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18. GCKR mutations in Japanese families with clustered type 2 diabetes.
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Tanaka D, Nagashima K, Sasaki M, Yamada C, Funakoshi S, Akitomo K, Takenaka K, Harada K, Koizumi A, and Inagaki N
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- Adult, Aged, Aged, 80 and over, Asian People, Case-Control Studies, Diabetes Mellitus, Type 2 epidemiology, Exons, Female, Genetic Linkage, Genetic Predisposition to Disease, Genome-Wide Association Study, Haplotypes, Humans, Male, Middle Aged, Mutagenesis, Insertional, Mutation, Missense, Pedigree, Phenotype, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, Sequence Analysis, DNA, Young Adult, Adaptor Proteins, Signal Transducing genetics, Diabetes Mellitus, Type 2 genetics
- Abstract
Objective: The aim was to investigate the genetic background of familial clustering of type 2 diabetes., Subjects and Methods: We recruited Japanese families with a 3-generation history of diabetes. Genome-wide linkage analysis was performed assuming an autosomal dominant model. Genes in the linkage region were computationally prioritized using Endeavour. We sequenced the candidate genes, and the frequencies of detected nucleotide changes were then examined in normoglycemic controls., Results: To exclude known genetic factors, we sequenced 6 maturity onset diabetes of the young (MODY) genes in 10 familial cases. Because we detected a MODY3 mutation HNF1A R583G in one case, we excluded this case from further investigation. Linkage analysis revealed a significant linkage region on 2p25-22 (LOD score=3.47) for 4 families. The 23.6-Mb linkage region contained 106 genes. Those genes were scored by computational prioritization. Eleven genes, i.e., top 10% of 106 genes, were selected and considered primary candidates. Considering their functions, we eliminated 3 well characterized genes and finally sequenced 8 genes. GCKR ranked highly in the computational prioritization. Mutations (minor allele frequency less than 1%) in exons and the promoter of GCKR were found in index cases of the families (3 of 18 alleles) more frequently than in controls (0 of 36 alleles, P=0.033). In one pedigree with 9 affected members, the mutation GCKR g.6859C>G was concordant with affection status. No mutation in other 7 genes that ranked highly in the prioritization was concordant with affection status in families., Conclusions: We propose that GCKR is a susceptibility gene in Japanese families with clustered diabetes. The family based approach seems to be complementary with a large population study., (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Published
- 2011
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19. Transduction Properties of Adenovirus Serotype 35 Vectors After Intravenous Administration Into Nonhuman Primates.
- Author
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Sakurai F, Nakamura SI, Akitomo K, Shibata H, Terao K, Kawabata K, Hayakawa T, and Mizuguchi H
- Abstract
Adenovirus serotype 35 (Ad35) vectors have shown promise as effective gene delivery vehicles. However, the transduction profiles of Ad35 vectors in conventional mice allow only a limited estimation of transduction properties of these vectors, because the mouse analog of the subgroup B Ad receptor, CD46, is restricted to the testis. In order to assess the transduction properties of Ad35 vectors more completely, we performed transduction experiments using cynomolgus monkeys, which ubiquitously express CD46 in a pattern similar to that in humans. In vitro transduction experiments demonstrated that cultured cells from the cynomolgus monkey were efficiently transduced with Ad35 vectors. In contrast, after intravenous administration into live monkeys hardly any evidence of Ad35 vector-mediated transduction was found in any of the organs, although Ad35 vector genomes were detected in various organs. Less severe histopathological abnormalities were found in the Ad35 vector-infused monkeys than in the conventional Ad5 vector-injected monkeys. In the latter, serious tissue damage and inflammatory responses, such as hepatocyte necrosis and lymphatic hyperplasia in the colon, were induced. Both Ad35 and Ad5 vectors caused similar hematological changes (increase in CD3
+ cells, and decrease in CD16+ cells and CD20+ cells) in peripheral blood cells. These results should provide valuable information for the clinical application of Ad35 vectors., (Copyright © 2008 The American Society of Gene Therapy. Published by Elsevier Inc. All rights reserved.)- Published
- 2008
- Full Text
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