1. Changes in the Transcription of Proliferation- and Apoptosis-Related Genes in Embryos in Women of Different Ages under the Influence of Extracellular Vesicles from Donor Follicular Fluid In Vitro.
- Author
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Nepsha OS, Burmenskaya OV, Akhmedova ZF, Romanov EA, Sysoeva AP, Goryunov KV, Shevtsova YA, Silachev DN, Makarova NP, and Kalinina EA
- Subjects
- Humans, Female, Adult, Gene Expression Regulation, Developmental, Cell Proliferation, Oocytes metabolism, Age Factors, Embryonic Development genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Extracellular Vesicles metabolism, Extracellular Vesicles genetics, Apoptosis genetics, Follicular Fluid metabolism, Blastocyst metabolism, Blastocyst cytology
- Abstract
We studied the influence of extracellular vesicles from the follicular fluid of a young donor on gene expression (MKI67, MYBL2, CCNB1, CCND1, CCNE1, CALM2, BAX, NDRG1, TP53I3, VEGF, VCAN, HAS2, CTSL2, PIBF1, RPL37, PFKP, GPX3, and AQP3) in embryos of women of different ages. According to nanoparticle tracking analysis data, the concentration of extracellular vesicles was 3.75±0.47×10
11 particles/ml and the mean particle size was 138.78±9.90 nm. During co-culturing of the follicular fluid extracellular vesicles with blastocysts of young women, we observed significantly increased expression of mRNA for genes CTSL2, CCND1, CCNE1, VEGF and reduced expression of BAX gene mRNA in comparison with embryos in women of late reproductive age. We hypothesized that addition of extracellular vesicles of the oocyte follicular fluid from a young donor to the culture medium of embryos could slow down apoptosis process typical of blastocyst cells in women above 36 years., (© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2024
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