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This study aims to determine the effect of adding duck egg yolk to skim milk-fructose extender on the motility and viability of Muscovy duck (Cairina moschata) spermatozoa at 5°C storage. Muscovy duck ejaculate was divided into four equal portions. The first portion was for the control group, which was extended in skim milk-fructose, while the second, third, and fourth portions were for treatment groups T1, T2, and T3, which were extended in skim milk-fructose with the addition of 2.5, 5.0, and 7.5% (v/v) duck egg yolk. Sperm motility and viability were evaluated every two hours until the motility percentage reached 40%. Data were analyzed using ANOVA and Duncan’s test. The results showed that spermatozoa motility and viability were higher (p <0.05) in groups T1, T2, and T3 compared to those of group T0 after 10 hours storage at 5°C. The highest percentage of spermatozoa motility and viability was in the T2 group compared to other groups (p <0.05). It could be concluded that the Muscovy duck semen extended in skim milk-fructose containing 2.5 to 7.5% (v/v) duck egg yolk met the requirements for artificial insemination when stored for up to 10 hours at 5°C. [ABSTRACT FROM AUTHOR]

This study aims to determine the effect of adding melon (Cucumis melo L.) juice to Lactated Ringer's-egg yolk extender on the intact plasma membrane (IPM) and morphological abnormalities of native rooster spermatozoa. Five ejaculate each from two native roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended 1:10 (v/v) in Lactated Ringer's-egg yolk without or with the addition of of 2, 4, and 8% (v/v) melon flesh juice, respectively for groups T0, T1, T2, and T3. Extended semen was stored in a refrigerator (5°C) and evaluated every two hours. The results showed that IPM of all groups decreased (p <0.05) consistently, lasting more than 40% for six hours in the T0 and T1 groups and four hours in the T2 and T3 groups. The percentage of spermatozoa morphological abnormalities in all groups stored for six hours was higher (p <0.05) than before storage. However, it was not significantly different (p >0.05) from that which had been stored for two and four hours. It was concluded that native rooster semen extended in Lactated Ringer's-egg yolk without (group T0) or with 2% melon flesh juice (group T1) and stored at 5°C retained spermatozoa plasma membrane integrity of more than 40% and morphological abnormalities of less than 20% for six hours. While the addition of 4 % (T2) and 8% (T3) melon flesh juice maintained the percentage of IPM and spermatozoa morphological abnormalities for only four hours. [ABSTRACT FROM AUTHOR]