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高感度 in situ ハイブリゼーション（ISH）法と高感度 PCR 法を用いて子宮頸部扁平上皮病変と腺病変での16型と18型のヒト乳頭種ウイルス感染頻度検索し、核への組み込みの頻度を ISH 法で決定した。16, 18型は PCR と ISH でともに、扁平上皮系病変のグレードが高まるにつれて感染頻度が上がった。浸潤扁平上皮癌のすべての例で ISH で組み込み型を示し、CIN1 の全てでエピソーム型を示した。腺系病変では PCR でも ISH でも16型より18型が多く検出され、そのほぼすべてが組み込み型だった。CIN2ではエピソーム型が多くCIN3では組み込み型が多かった。これらから16, 18型の核への組み込みは扁平上皮病変でも腺病変でも癌の初期に現れる変化、あるいは癌への進展を強く示唆する所見と考えられた。Highly sensitive in situ hybridization (ISH) and highly sensitive PCR methods were used to determine the infection of HPV type 16 (HPV 16) and type 18 (HPV 18) and the integration of them into the cells of squamous and glandular lesions of the uterine cervix. The frequency of HPV infection detected by ISH and PCR increased with progression of squamous lesions. All cases of ICC showed the integrated pattern of positive signals with ISH and all positive cases in CIN1 showed the episomal pattern. In CIN2 episomal patterns were more frequent than integrated pattern and in CIN3 integrated pattern were more frequent. Glandular lesions showed a greater frequency of HPV 18 than HPV 16 on nested PCR and ISH, and almost all of ISH-positive cases showed the integrated pattern. These data suggested that integration of HPV 16 and 18 is required for carcinogenesis in both squamous cell carcinoma and adenocarcinoma of the uterine cervix.

金沢大学大学院医学系研究科保健学専攻, Little is known about the functions of two different β5 integrins: repeated-FNK (FNKFNK764–769) and single-FNK (FNK764–766) amino acid sequences in the cytoplasmic domain. We examined whether they occurred as germ line mutations or somatic mutations associated with neoplastic transformation, and whether there were functional alterations. Out of six cultured cell lines, only KATO-III cells had the single-FNK β5 sequence. The single-FNK β5 was found in 9 out of 79 patients with colon carcinoma, but no somatic mutations were detected in cancerous tissues. CHO cells were transformed with expression vectors containing single-FNK or repeated-FNK β5 cDNA, which were derived from KATO-III cells. CHO cells transfected with single-FNK and repeated-FNK showed similar adhesiveness to, and proliferative activity on, vitronectin substrates.