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Background: Vitrification is a technique of cryopreservation that has been proposed as a promising alternative method for the preservation of oocytes, embryos and gonadal tissue., Objective: To determine the effect of different antioxidants on post-thaw viability, morphology of retrieved oocytes and histology of vitrified ovarian tissue., Materials and Methods: Four different antioxidants [i.e., resveratrol (20 uM), ZnSO 4 (500 uM), curcumin (25 uM) and quercetin (1 uM)] were evaluated after their addition to the vitrification and warming media for their effects on the viability and morphology of retrieved oocytes and the histology of vitrified ovarian tissue., Results: The number of oocytes retrieved from ovarian tissue from the above mentioned antioxidants and vitrified control were 34, 41, 26, 31 and 46 respectively. Among these the number of viable oocytes were found to be 24 (70.6%), 30 (73.1 %), 20 (76.9%), 26 (83.9%) and 33 (71.7%) and the number of oocytes found morphologically normal were 24 (70.6%), 26 (63.4%), 18 (69.2%), 21 (67.7%) and 34 (73.9%) for the above mentioned different antioxidants and vitrified control, respectively. Non-significant (P. > 0.05) differences were found between different treatment groups. Histomorphological evaluation of the ovarian cortical tissue showed that the percentage of intact follicles was significantly (P < 0.05) higher in the fresh control (84.19±3.9) than in other groups. Non-significant differences were found between resveratrol (50.2±5.5), curcumin (48.7±5.7), quercetin (51.6±4.8) and the vitrified control (42.7±6.1) groups; however, the ZnSO4 supplemented group (23.1±8.54) differed significantly (P < 0.05) from other antioxidant groups but was non-significant (P > 0.05) with the vitrified control group (42.7±6.1)., Conclusion: The addition of antioxidants resveratrol, curcumin and quercetin at these concentrations tended to non-significantly improve the follicular integrity after vitrification. Doi.org/10.54680/fr24410110212.

The objective of the study was to evaluate the sustainability of the grazing and feeding resources of the sheep in Kashmir, India. The study was conducted in the Ganderbal District of Kashmir to evaluate sustainability, along with the scenario of grazing pattern and feeding system of the sheep during the non-migratory period. The district was divided into three production zones based on altitude, i.e., zone I (high altitude), zone II (medium altitude), and zone III (low altitude). A total of 360 sheep farmers were interviewed from the three zones that consist of 9 blocks, with 40 sheep farmers interviewed from each block. Majority of the sheep farmers (75.00%) follow a semi-migratory type of production system, use forest, and low-mountain areas for grazing purposes (48.88%) and were having medium to high availability of grazing lands. The main feeding source available to majority of the sheep farmers (60.00%) was found to be crop residues and fodder grasses, with local markets (60.00%) and fellow farmers (71.11%) being the main source of procuring concentrates and fodders. Hybrid entropy and TOPSIS method were used for the evaluation of sustainability of grazing and feeding system in the area. The most influencing factors for sustainability of grazing pattern and feeding source were found to be production system followed and the major feed sources available respectively. Most of the variables included in the grazing system were ideally best in zone I and of feeding system were found to be ideally best in zone III. There is a need for improvement in the parameters of the grazing pattern in zone III, i.e., in low-altitude areas or planes where grazing lands are shrinking at an alarming rate. The parameters under the feeding system need due consideration in zone I, i.e., in the high altitudes where the socio-economic situation of the people is comparatively poor than the other two zones., (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)

Men's dominant ally, best defense defendant, therefore, the pre-eminent peril detector, dogs became a threat for the humanity inflicting a fatal disease of rabies. Dog bites and open garbage dumps became additional and apparent and are serious and sometimes underestimated public health dilemma in the Kashmir region. The aesthetic, primarily health care and psycho-sociological consequences of trauma caused by a dog bites repeatedly burden the standard of lifetime pertaining to the affected persons and their family. The present study was carried in Srinagar district. Diverse sampling plan / data collection strategies were formulated to fulfill the set of objectives. To assess the epidemiological pattern, secondary source of information viz. Shri Maharaja Hari Singh hospital, which shouldered the relevant cases, was utilized. Further, people perception of the subject was assessed using a predesigned interview schedule from respondents selected by appropriate methods. West zone had recorded the highest number of cases (28.95 %), then the south zone, followed by the north zone and the east zone. Majority victims were males (74.27 %) followed by females (25.72 %) [χ 2 = 4.442, p = 0.219). Most of them belonged to 30-40 (26.88 %) year old groups, followed by 20-30 (22.36 %), 10-20 (16.03 %) and so on. The time of exposure of victims was evening (62.90 %) [χ 2 = 30.342, p = 0.001]. The bulk number of cases had bite on legs (56.60 %). Majority had category 3 bites (70.16 %) and the majority had received immunoglobin (82.85 %) [χ 2 = 29.56, p = 0.001]. The summer season (29.30 %) had also recorded the highest number of cases followed by autumn season (27.82 %) irrespective of each determinant. The Anti rabies clinic of SMHS has also recorded two rabies deaths during this period. The majority people (72.50 %) were illiterate and belonging to lower sections of society. About all those diseases that attack humans, rabies is an example of the best known in Srinagar. Its notoriety is peculiar when assessed on its influence medially, on humans who are belonging to the susceptible divisions of the society due to lack of discernment. The lack of complete perception might decipher into higher mortality linked to rabies., (Copyright © 2020 Elsevier Ltd. All rights reserved.)