1. Two FtsH Proteases Contribute to Fitness and Adaptation of Pseudomonas aeruginosa Clone C Strains
- Author
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Shady Mansour Kamal, Morten Levin Rybtke, Manfred Nimtz, Stefanie Sperlein, Christian Giske, Janja Trček, Julien Deschamps, Romain Briandet, Luciana Dini, Lothar Jänsch, Tim Tolker-Nielsen, Changhan Lee, Ute Römling, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Microbiology, Tumor and Cell Biology, Ajouter cet établissement, Future University in Egypt, Costerton Biofilm Center [Copenhagen], Department of Immunology and Microbiology [Copenhagen], Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU)-Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Department of Cellular Proteomics, Helmholtz Centre for Infection Research (HZI), Division of Clinical Microbiology - Department of Laboratory Medicine, Karolinska Institute, Department of Biology - Faculty of Natural Sciences and Mathematics, University of Maribor, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COMUE), Department of Biological and Environmental Sciences and Technologies (DiSTeBA), Università del Salento, and Römling, Ute
- Subjects
Microbiology (medical) ,FtsH protease ,Proteases ,[SDV]Life Sciences [q-bio] ,Mutant ,lcsh:QR1-502 ,Virulence ,secondary metabolite ,autolysis ,Biology ,medicine.disease_cause ,heat shock factor RpoH ,Microbiology ,lcsh:Microbiology ,Secondary metabolite ,03 medical and health sciences ,Genomic island ,Protein biosynthesis ,medicine ,Phenazine ,Gene ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,Original Research ,clone C strains ,Genetics ,0303 health sciences ,030306 microbiology ,Pseudomonas aeruginosa ,phenazine ,Clone C strains ,Heat shock factor ,virulence ,phenazine,secondary metabolite ,Heat shock factor RpoH ,Autolysis - Abstract
Pseudomonas aeruginosa is an environmental bacterium and a nosocomial pathogen with clone C one of the most prevalent clonal groups. The P. aeruginosa clone C specific genomic island PACGI-1 harbors a xenolog of ftsH encoding a functionally diverse membrane-spanning ATP-dependent metalloprotease on the core genome. In the aquatic isolate P. aeruginosa SG17M, the core genome copy ftsH1 significantly affects growth and dominantly mediates a broad range of phenotypes, such as secretion of secondary metabolites, swimming and twitching motility and resistance to aminoglycosides, while the PACGI-1 xenolog ftsH2 backs up the phenotypes in the ftsH1 mutant background. The two proteins, with conserved motifs for disaggregase and protease activity present in FtsH1 and FtsH2, have the ability to form homo- and hetero-oligomers with ftsH2 distinctively expressed in the late stationary phase of growth. However, mainly FtsH1 degrades a major substrate, the heat shock transcription factor RpoH. Pull-down experiments with substrate trap-variants inactive in proteolytic activity indicate both FtsH1 and FtsH2 to interact with the inhibitory protein HflC, while the phenazine biosynthesis protein PhzC was identified as a substrate of FtsH1. In summary, as an exception in P. aeruginosa, clone C harbors two copies of the ftsH metallo-protease, which cumulatively are required for the expression of a diversity of phenotypes.
- Published
- 2019