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Recently, there has been significant research on plant-based meat as a substitute for animal meat. However, developing meat analogues with suitable techno-functional properties and a meat-like texture presents several challenges. In lieu of animal meat, plant-based meats offer a sustainable and healthy alternative. The nutritional and textural properties of meat analogues are mostly influenced by the specific type of proteins utilized and the various processing techniques implemented. Despite this, alternative proteins sourced from various plants such as legumes, pulses, and cereals, continue to be underutilized, with limited research done on their use in meat analogue development. The sustainability of production technologies and the selection of proteins can have a direct impact on both cost effectiveness and consumer of meat analogues. The challenges associated with the development of gluten-free meat analogues are currently being examined. Moreover, the extraction techniques employed in the isolation of plant proteins are of paramount importance, as they contribute to the organoleptic and textural attributes of meat analogues. By utilizing advanced technologies to sustainably utilize diverse plant proteins, it is feasible to conquer the challenges encountered in the production of plant-based meat products that are palatable to both consumers and the food industry. In this review we have discussed the functional roles of various ingredients and additives for enhancing nutritional and textural properties of meat analogues and current technologies used for texturizing plant-based meat analogues. Further we have discussed various analytical methods for testing the quality attributes of meat analogues and technological and consumer perception/acceptability-related challenges and opportunities. [ABSTRACT FROM AUTHOR]

With hyperlipidemia posing a significant cardiovascular risk, innovative strategies are essential to unlock new therapeutic possibilities. Probiotic fermentation of milk proteins offers a natural and effective means to produce peptides with hypolipidemic properties, providing a promising approach to lowering lipid levels and reducing cardiovascular risk. In this study, fermented cattle milk (FCTM), fermented camel milk (FCM), fermented goat milk (FGM), and fermented sheep milk (FSM) were produced using a total of five probiotic bacterial strains to investigate the release of bioactive peptides (BAPs) with hypolipidemic potential via in vitro inhibitory activity toward pancreatic lipase (PL) during a 14-day refrigerated storage study. The PL inhibitory activities of these fermented milk (FM) varied according to the types of probiotic strains and milk types used. Overall, the Pediococcus pentosaceus MF000957 (PP-957) strain showed the highest PL inhibitory activity spanning across all milk types, and therefore, PP-957-derived fermented samples were analyzed for BAP identification by LCMS-QTOF. The identified BAPs were further analyzed using in silico and bioinformatics approaches for bioactivity prediction, molecular docking, and drug pharmacokinetic studies. Overall, four peptides derived from FCTM, one from FCM, and two peptides common in FGM and FSM were predicted as active PL inhibitors based on their binding energy and number of binding sites on the PL enzyme. All peptides were non-toxic, non-carcinogenic, and had appropriate drug-like properties. The outcomes of this study suggest that FM-derived peptides from animal milk are anticipated to be useful for combating hypercholesterolemia. [ABSTRACT FROM AUTHOR]

The development of novel protein sources to compensate for the expected future shortage of traditional animal proteins due to their high carbon footprint is a major contemporary challenge in the agri-food industry currently. Therefore, both industry and consumers are placing a greater emphasis on plant proteins as a sustainable source of protein to meet the growing nutritional demand of ever increasing population. In addition to being key alternatives, many plant-based foods have biological properties that make them potentially functional or health-promoting foods, particularly physiologically active peptides and proteins accounting for most of these properties. This review discusses the importance of plant-based protein as a viable and sustainable alternative to animal proteins. The current advances in plant protein isolation and production and characterization of bioactive hydrolysates and peptides from plant proteins are described comprehensively. Furthermore, the recent research on bioactivities and bioavailability of plant protein-derived bioactive peptides is reviewed briefly. The limitations of using bioactive peptides, regulatory criteria, and the possible future applications of plant protein-derived bioactive peptides are highlighted. This review may help understand plant proteins and their bioactive peptides and provide valuable suggestions for future research and applications in the food industry. [ABSTRACT FROM AUTHOR]

The effect of packaging materials and storage period on the chemical composition, microbial load of Madidi- a thick porridge mad e from millet was investigated. The samples were packed in Madidi leave, plastic container, nylon, aluminum foil and banana leaves, respectively. The chemical and microbial analyses were determined at 0, 7 and 14 days of storage period. Sensory evaluation was done using 50 panelists. The samples were statistically analyzed using ANOVA and the means were separated by Duncan multiple test at p<0.05 significance level. The results obtained at day 0 are pH (4.19), TTA (0.40), Moisture content (9.78%), ash (2.02%), fibre (3.50%), protein (3.89%), fat (0.83%) and carbohydrate (70.23%). The chemical composition of samples in plastic container and nylon were significantly (p>0.05) higher than other samples. Madidi packed in leaves and plastic container had the highest microbial load. The result showed that the pH, TTA, Protein, fat, fibre, ash and carbohydrate decreased significantly, while the moisture content and microbial load increased after the storage period. The sensory evaluation showed that samples packed in plastic containers were highly accepted by the panelists at the end of the storage in terms of the sensory attributed evaluated. [ABSTRACT FROM AUTHOR]

[Display omitted] • Metabolomics was used to characterize digestion differences between MS and YS. • More bioactive compounds were released in gastric digestion than in intestinal digestion. • YS samples contained more total sugars than MS samples. • Reducing power and anti-inflammatory activity decreased with the increase of total organic and amino acids content. • Pancreatic lipase and cholesterol esterase inhibition activities improved with the increase of total organic acids content. The aim of the present study was to comparatively investigate the relative phytochemical profiles (phenolic content, organic and amino acids, free sugars, and other metabolites using metabolomics approach), and bioactive potentials of young (YS) and mature soybean (MS) upon in vitro simulated gastrointestinal digestion (SGID). Cumulatively, a total of 198 metabolites were identified in MS and YS, 119 metabolites in undigested YS, and a total of 136 metabolites in undigested MS, which further increased to 156 and 152 in YS and MS upon SGID, respectively. Gastric digesta of both YS and MS exhibited higher inhibitory properties towards α-amylase and DPP-IV enzymes than their intestinal digesta. Furthermore, the intestinal digesta of MS showed higher antioxidant and anti-inflammatory activities compared to the YS intestinal digesta. Overall, the results suggested that the gastrointestinal digestion of YS and MS displayed distinctive metabolic profiles together with varied bioactive potentials. [ABSTRACT FROM AUTHOR]