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Our investigation within the framework of this study is carried out on three marine species fished off in Moroccan coasts namely: a fish, the sole (Solea Vulgaris), a cephalopod, the cuttlefish (Sepia Officinalis) and a crustacean, the pink shrimp (Parapenaeus Longirostris). The aims of this study is to assess the level of concentration in these three marine species, of three metallic trace elements: Lead (Pb), Cadmium (Cd) and Mercury (Hg) and giving a reflection on the way in which Moroccan deep-sea fishing professionals can join a food safety management system to ensure the safety of their product and promote their competitiveness. Sampling is carried out at the port of Agadir city after landing of the deep-sea fishing boats which operate beyond 10 nautical miles, offshoring the seabed of Agadir for pink shrimp (Parapenaeus Longirostris), and in Dakhla city offshore for sole (Solea Vulgaris) and cuttlefish (Sepia Officinalis). Trace metal analysis were performed on individual muscles belonging to the three species. The results of our investigations show that the Pb content varies between 0.01 ppm and 0.27 ppm in Solea Vulgaris; between 0.015 ppm and 0.16 ppm in Sepia Officinalis and between 0.022 ppm and 0.38 ppm for Parapenaeus Longirostris. For the Cd, the concentrations vary between 0.0047 ppm and 0.050 ppm in Solea Vulgaris; between 0.0025 ppm and 0.254 ppm in Sepia Officinalis and between 0.0065 ppm and 0.11 ppm in Parapenaeus Longirostris. Concerning Hg, the contents vary between 0.0006 ppm and 0.075 ppm in Solea Vulgaris; between 0.0008 ppm and 0.05 ppm in Sepia Officinalis and between 0.0016 ppm and 0.09 ppm in Parapenaeus Longirostris. It appears from this study that the metal contents detected at the level of the three species of seafood investigated are below the regulatory thresholds. These results can be considered as the first basis for validating the control measure linked to the absence of metallic contamination of the three species in the sites studied.

Halophilic bacteria are a group of microorganisms living in saline environments and in some cases need the salinity to survive. Furthermore,these bacteria species have the potential for interesting and promising applications. In fact, this is due not only to their ability to adapt to extreme physico-chemical conditions but also because many of them they produce interesting substances for the industry such as extracellular hydrolytic enzymes.The present study aims to isolate and characterize halophilic bacteria producing hydrolase enzymes from twosalinesin Sidi Moussa-Oualidia complex (Morocco) and to screen their potential to produce hydrolase enzymes. In this study, 15 halophilic bacteria were identified and analyzed for their ability to produce extracellular enzymes. The phylogenetic analysis based on 16S rDNA sequencing indicated that the 15 isolated strains belong to the genera Bacillus, Oceanobacillus and Virgibacillus. The study of enzymatic activity revealed that all isolates studied were capable of producing at least one extracellular hydrolytic enzyme of which 9 produced amylase, 6 cellulase, 13 DNase, 11 lipase, and 8 protease. In addition to their ability to produce extracellular hydrolytic enzymes, our isolates have demonstrated their potential adaption to extreme Physico-chemical conditions. These properties could allow them to be used for many industrial applications.

Tetraclinis articulata (Vahl) Masters (TA), a member of the Cupressaceae family, is frequently used in traditional medicine to treat various health problems in Al Hoceima Province. Our study aimed to determine the content of polyphenols and flavonoids, and to evaluate the biological antioxidant activities using DPPH and FRAP tests. At the same time, we assessed the anti-inflammatory activity of various extracts of TA from Al Hoceima, using the BSA method for each of the extracts studied. The results reveal that ethyl acetate extracts (28.11 mg EAG/g ES), followed by ethanolic extracts (22.42 mg EAG/g ES), show higher quantities than the other extracts. Regarding flavonoids, ethyl acetate extract (90 mg EQ/g ES) and ethanolic extracts (64 mg EQ/g ES) showed the highest concentrations. For antioxidant activity, ethyl acetate and ethanolic extracts showed significant activity, while for anti-inflammatory activity, ethyl acetate and hexanolic extracts showed more marked activity than ethanolic and dichloromethanic extracts. This study reveals that Tetraclinis articulata leaf extracts are an effective antioxidant and natural anti-inflammatory agent. The results indicate the efficacy of TA leafs extracts from the province of Al Hoceima.

Introduction: Nosocomial infections associated with a carbapenem-resistant Acinetobacter baumannii (CRAB) are considered as a significant challenge in the Intensive care units’ (ICU) critically ill patients. Lack of new antimicrobial agents to treat infections caused by this multi-drug resistant microorganism reinforces the exploration of novel strategies to encounter and control this infection. Thus, the aim of the study was to evaluate, for the first time, the antibacterial effectiveness of the Lavandula mairei Humbert essential oil (EO) against a CRAB isolated from the hospital environment of the two ICUs. Materials and Methods: Carbapenemase encoding genes were detected by the polymerase chain reactions and sequencing methods. The chemical composition of Lavandula mairei (EO) was determined by a gas chromatography-mass spectrometry analysis, and its antibacterial activity was evaluated by a disk diffusion and broth microdilution methods. Results: The OXA-23 and NDM-1 variants were revealed in the selected strains. Twenty-six compounds were identified in the Lavandula mairei EO, in which carvacrol was the most dominant one (77.32%). All the tested CRAB strains showed a wide sensitivity to the oil, with the inhibition zones’ diameters ranging from 28.67±1.15 mm to 40±2.00 mm, and a bactericidal effect proved by the similarity of minimal inhibitory and bactericidal concentrations values (0.39-3.125 µL/ml). Conclusion: These findings remain very promising for the future use of Lavandula mairei EO as a source of the natural antibacterial agents.

The aim of the study was to investigate the antibacterial and antibiofilm activities of Lavandula mairei and Thymus leptobotrys essential oils against Extended spectrum β-lactamase producing Enterobacteriaceae, responsible of nosocomial infections. PCR and sequencing methods detected three different resistance genes (blaSHV-42, blaSHV-12 et blaCTXM-15) in the studied strains. Gas chromatography-mass spectrometry analysis highlighted the predominance of carvacrol in both Lavandula mairei and Thymus leptobotrys essential oils. Their antibacterial activity was conducted by disk diffusion and microdilution methods. Great sensitivity of the multidrug resistant strains was noted for the two essential oils with inhibition zones' diameters ranging from 13.3±2.3 to 32.3±1.4 mm (Thymus leptobotrys) and from 22 to 34.66±0.57 mm (Lavandula mairei), and low minimal and bactericidal concentrations varying from 1.56 to 6.25 mg/mL (CMI) and from 1.56 to 12.5 mg/mL (CMB) for both essential oils. Four strains out of six, considered as the strongest biofilm producers by congo red agar and crystal violet methods, were chosen to evaluate biofilm inhibition and eradication effects of Lavandula mairei and Thymus leptobotrys essential oils. In most cases, these latter inhibited considerably the biofilm formation of the targeted strains with proportions greater than 80%. The eradication effect was lower, with rates not higher than 50%. Thus, these essential oils could be used as an effective alternative approach to prevent biofilm formation of multidrug resistant Enterobacteriaceae. To our knowledge, no previous works have reported the antibiofilm activity of Lavandula mairei and Thymus leptobotrys essential oils against these multidrug resistant bacteria. [ABSTRACT FROM AUTHOR]

Abstract In order to use purified wastewater on watering green spaces of Agadir city, we conducted this study to assess the bacteriological and physicochemical quality of treated wastewater in Mzar treatment plant. To realize these objectives, we analyzed four types of waters: raw water (RW), decanted water, purified water and purified water treated by the ultraviolet radiation (PWUV). The biologically analyzed parameters are fecal coliforms (FC) and fecal enterococci (FE). The physicochemical parameters are: pH, electrical conductivity, chemical oxygen demand (COD), five-day biological oxygen demand (BOD5) and total suspended solids (TSS). The results showed a high level of bacterial contamination in all types of waters, and the average loads of FC were between 4.71 × 106 CFU/100 mL (RW) and 1.45 × 103 CFU/100 mL (PWUV), while loads of FE were between 2.62 × 105 CFU/100 mL (RW) and 7.92 CFU/100 mL (PWUV). The physicochemical parameters show a pH close to neutrality and a high electrical conductivity (3261 μS/cm) in PWUV. In all stages of wastewater treatment, values of COD, BOD5 and TSS were under the limit values recommended by Moroccan standards. Except for the electrical conductivity and FC, all the studied parameters were under the limit values requested by the Moroccan standards. The microbiological quality of PWUV is unstable, and if these waters are reused, they can affect the microbiological and physicochemical quality of green spaces and groundwater.