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17 results on '"Ainsworth, Madison J."'

1. Hypothermic and cryogenic preservation of cardiac tissue-engineered constructs

Author

Experimentele Afd. Cardiologie 2, Regenerative Medicine and Stem Cells, Orthopaedie Onderzoek, Team Medisch, Circulatory Health, Onderzoek Cardiovasculair Reg. Med., Onderzoek Algemene Cardiologie, Janssen, Jasmijn, Chirico, Nino, Ainsworth, Madison J, Cedillo-Servin, Gerardo, Viola, Martina, Dokter, Inge, Vermonden, Tina, Doevendans, Pieter A, Serra, Margarida, Voets, Ilja K, Malda, Jos, Castilho, Miguel, van Laake, Linda W, Sluijter, Joost P G, Sampaio-Pinto, Vasco, van Mil, Alain, Experimentele Afd. Cardiologie 2, Regenerative Medicine and Stem Cells, Orthopaedie Onderzoek, Team Medisch, Circulatory Health, Onderzoek Cardiovasculair Reg. Med., Onderzoek Algemene Cardiologie, Janssen, Jasmijn, Chirico, Nino, Ainsworth, Madison J, Cedillo-Servin, Gerardo, Viola, Martina, Dokter, Inge, Vermonden, Tina, Doevendans, Pieter A, Serra, Margarida, Voets, Ilja K, Malda, Jos, Castilho, Miguel, van Laake, Linda W, Sluijter, Joost P G, Sampaio-Pinto, Vasco, and van Mil, Alain

Published
2024

2. Hypothermic and cryogenic preservation of cardiac tissue-engineered constructs

Author

Janssen, Jasmijn, Chirico, Nino, Ainsworth, Madison J, Cedillo-Servin, Gerardo, Viola, Martina, Dokter, Inge, Vermonden, Tina, Doevendans, Pieter A, Serra, Margarida, Voets, Ilja K, Malda, Jos, Castilho, Miguel, van Laake, Linda W, Sluijter, Joost P G, Sampaio-Pinto, Vasco, van Mil, Alain, Janssen, Jasmijn, Chirico, Nino, Ainsworth, Madison J, Cedillo-Servin, Gerardo, Viola, Martina, Dokter, Inge, Vermonden, Tina, Doevendans, Pieter A, Serra, Margarida, Voets, Ilja K, Malda, Jos, Castilho, Miguel, van Laake, Linda W, Sluijter, Joost P G, Sampaio-Pinto, Vasco, and van Mil, Alain

Abstract

Cardiac tissue engineering (cTE) has already advanced towards the first clinical trials, investigating safety and feasibility of cTE construct transplantation in failing hearts. However, the lack of well-established preservation methods poses a hindrance to further scalability, commercialization, and transportation, thereby reducing their clinical implementation. In this study, hypothermic preservation (4 °C) and two methods for cryopreservation ( i.e., a slow and fast cooling approach to -196 °C and -150 °C, respectively) were investigated as potential solutions to extend the cTE construct implantation window. The cTE model used consisted of human induced pluripotent stem cell-derived cardiomyocytes and human cardiac fibroblasts embedded in a natural-derived hydrogel and supported by a polymeric melt electrowritten hexagonal scaffold. Constructs, composed of cardiomyocytes of different maturity, were preserved for three days, using several commercially available preservation protocols and solutions. Cardiomyocyte viability, function (beat rate and calcium handling), and metabolic activity were investigated after rewarming. Our observations show that cardiomyocytes' age did not influence post-rewarming viability, however, it influenced construct function. Hypothermic preservation with HypoThermosol® ensured cardiomyocyte viability and function. Furthermore, fast freezing outperformed slow freezing, but both viability and function were severely reduced after rewarming. In conclusion, whereas long-term preservation remains a challenge, hypothermic preservation with HypoThermosol® represents a promising solution for cTE construct short-term preservation and potential transportation, aiding in off-the-shelf availability, ultimately increasing their clinical applicability.

Published
2024

3. Covalent Grafting of Functionalized MEW Fibers to Silk Fibroin Hydrogels to Obtain Reinforced Tissue Engineered Constructs

Author

Afd Pharmaceutics, Equine Musculoskeletal Biology, CS_Locomotion, Locomotion - Equine Sciences, Viola, Martina, Ainsworth, Madison J, Mihajlovic, Marko, Cedillo-Servin, Gerardo, van Steenbergen, Mies J, van Rijen, Mattie, de Ruijter, Mylène, Castilho, Miguel, Malda, Jos, Vermonden, Tina, Afd Pharmaceutics, Equine Musculoskeletal Biology, CS_Locomotion, Locomotion - Equine Sciences, Viola, Martina, Ainsworth, Madison J, Mihajlovic, Marko, Cedillo-Servin, Gerardo, van Steenbergen, Mies J, van Rijen, Mattie, de Ruijter, Mylène, Castilho, Miguel, Malda, Jos, and Vermonden, Tina

Published
2024

4. Covalent Grafting of Functionalized MEW Fibers to Silk Fibroin Hydrogels to Obtain Reinforced Tissue Engineered Constructs

Author

Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Viola, Martina, Ainsworth, Madison J., Mihajlovic, Marko, Cedillo-Servin, Gerardo, van Steenbergen, Mies J., van Rijen, Mattie, de Ruijter, Mylène, Castilho, Miguel, Malda, Jos, Vermonden, Tina, Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Viola, Martina, Ainsworth, Madison J., Mihajlovic, Marko, Cedillo-Servin, Gerardo, van Steenbergen, Mies J., van Rijen, Mattie, de Ruijter, Mylène, Castilho, Miguel, Malda, Jos, and Vermonden, Tina

Published
2024

6. Covalent Protein Immobilization on 3D-Printed Microfiber Meshes for Guided Cartilage Regeneration

Author

Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Other research (not in main researchprogram), Ainsworth, Madison J., Lotz, Oliver, Gilmour, Aaron, Zhang, Anyu, Chen, Michael J., McKenzie, David R., Bilek, Marcela M.M., Malda, Jos, Akhavan, Behnam, Castilho, Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Other research (not in main researchprogram), Ainsworth, Madison J., Lotz, Oliver, Gilmour, Aaron, Zhang, Anyu, Chen, Michael J., McKenzie, David R., Bilek, Marcela M.M., Malda, Jos, Akhavan, Behnam, and Castilho

Published
2023

7. Hypothermic and cryogenic preservation of cardiac tissue-engineered constructsElectronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3bm01908j

Author

Janssen, Jasmijn, Chirico, Nino, Ainsworth, Madison J., Cedillo-Servin, Gerardo, Viola, Martina, Dokter, Inge, Vermonden, Tina, Doevendans, Pieter A., Serra, Margarida, Voets, Ilja K., Malda, Jos, Castilho, Miguel, van Laake, Linda W., Sluijter, Joost P. G., Sampaio-Pinto, Vasco, and van Mil, Alain

Abstract

Cardiac tissue engineering (cTE) has already advanced towards the first clinical trials, investigating safety and feasibility of cTE construct transplantation in failing hearts. However, the lack of well-established preservation methods poses a hindrance to further scalability, commercialization, and transportation, thereby reducing their clinical implementation. In this study, hypothermic preservation (4 °C) and two methods for cryopreservation (i.e., a slow and fast cooling approach to −196 °C and −150 °C, respectively) were investigated as potential solutions to extend the cTE construct implantation window. The cTE model used consisted of human induced pluripotent stem cell-derived cardiomyocytes and human cardiac fibroblasts embedded in a natural-derived hydrogel and supported by a polymeric melt electrowritten hexagonal scaffold. Constructs, composed of cardiomyocytes of different maturity, were preserved for three days, using several commercially available preservation protocols and solutions. Cardiomyocyte viability, function (beat rate and calcium handling), and metabolic activity were investigated after rewarming. Our observations show that cardiomyocytes’ age did not influence post-rewarming viability, however, it influenced construct function. Hypothermic preservation with HypoThermosol® ensured cardiomyocyte viability and function. Furthermore, fast freezing outperformed slow freezing, but both viability and function were severely reduced after rewarming. In conclusion, whereas long-term preservation remains a challenge, hypothermic preservation with HypoThermosol® represents a promising solution for cTE construct short-term preservation and potential transportation, aiding in off-the-shelf availability, ultimately increasing their clinical applicability.

Published
2024
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10. NEXT GENERATION BIOENGINEERED HUMAN MYOCARDIUM

Author

CS_Locomotion, Equine Musculoskeletal Biology, van Mil, Alain, Castilho, Miguel, Chirico, Nino, Ainsworth, Madison J., Dokter, Inge, Maas, Renee G. C., Buikema, Jan Willem, Malda, Jos, Sluijter, Joost P. G., CS_Locomotion, Equine Musculoskeletal Biology, van Mil, Alain, Castilho, Miguel, Chirico, Nino, Ainsworth, Madison J., Dokter, Inge, Maas, Renee G. C., Buikema, Jan Willem, Malda, Jos, and Sluijter, Joost P. G.

Published
2022

11. Covalent Protein Immobilization on 3D‐Printed Microfiber Meshes for Guided Cartilage Regeneration.

Author

Ainsworth, Madison J., Lotz, Oliver, Gilmour, Aaron, Zhang, Anyu, Chen, Michael J., McKenzie, David R., Bilek, Marcela M.M., Malda, Jos, Akhavan, Behnam, and Castilho, Miguel

Subjects
*CARTILAGE regeneration, *GUIDED tissue regeneration, *MICROFIBERS, *TRANSFORMING growth factors, *TRANSFORMING growth factors-beta, *ARTICULAR cartilage
Abstract

Current biomaterial‐based strategies explored to treat articular cartilage defects have failed to provide adequate physico‐chemical cues in order to guide functional tissue regeneration. Here, it is hypothesized that atmospheric‐pressure plasma (APPJ) treatment and melt electrowriting (MEW) will produce microfiber support structures with covalently‐immobilized transforming growth factor beta‐1 (TGFβ1) that can stimulate the generation of functional cartilage tissue. The effect of APPJ operational speeds to activate MEW polycaprolactone meshes for immobilization of TGFβ1 is first investigated and chondrogenic differentiation and neo‐cartilage production are assessed in vitro. All APPJ speeds test enhanced hydrophilicity of the meshes, with the slow treatment speed having significantly less CC/CH and more COOH than the untreated meshes. APPJ treatment increases TGFβ1 loading efficiency. Additionally, in vitro experiments highlight that APPJ‐based TGFβ1 attachment to the scaffolds is more advantageous than direct supplementation within the medium. After 28 days of culture, the group with immobilized TGFβ1 has significantly increased compressive modulus (more than threefold) and higher glycosaminoglycan production (more than fivefold) than when TGFβ1 is supplied through the medium. These results demonstrate that APPJ activation allows reagent‐free, covalent immobilization of TGFβ1 on microfiber meshes and, importantly, that the biofunctionalized meshes can stimulate neo‐cartilage matrix formation. This opens new perspectives for guided tissue regeneration. [ABSTRACT FROM AUTHOR]

Published
2023
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12. Fiber Scaffold Patterning for Mending Hearts: 3D Organization Bringing the Next Step

Author

Kristen, Marleen, Ainsworth, Madison J, Chirico, Nino, van der Ven, Casper F T, Doevendans, Pieter A, Sluijter, Joost P G, Malda, Jos, van Mil, Alain, Castilho, Miguel, Kristen, Marleen, Ainsworth, Madison J, Chirico, Nino, van der Ven, Casper F T, Doevendans, Pieter A, Sluijter, Joost P G, Malda, Jos, van Mil, Alain, and Castilho, Miguel

Abstract

Heart failure (HF) is a leading cause of death worldwide. The most common conditions that lead to HF are coronary artery disease, myocardial infarction, valve disorders, high blood pressure, and cardiomyopathy. Due to the limited regenerative capacity of the heart, the only curative therapy currently available is heart transplantation. Therefore, there is a great need for the development of novel regenerative strategies to repair the injured myocardium, replace damaged valves, and treat occluded coronary arteries. Recent advances in manufacturing technologies have resulted in the precise fabrication of 3D fiber scaffolds with high architectural control that can support and guide new tissue growth, opening exciting new avenues for repair of the human heart. This review discusses the recent advancements in the novel research field of fiber patterning manufacturing technologies for cardiac tissue engineering (cTE) and to what extent these technologies could meet the requirements of the highly organized and structured cardiac tissues. Additionally, future directions of these novel fiber patterning technologies, designs, and applicability to advance cTE are presented.

Published
2020

13. Fiber Scaffold Patterning for Mending Hearts: 3D Organization Bringing the Next Step

Author

LS Equine Muscoskeletal Biology, dES RMSC, Kristen, Marleen, Ainsworth, Madison J, Chirico, Nino, van der Ven, Casper F T, Doevendans, Pieter A, Sluijter, Joost P G, Malda, Jos, van Mil, Alain, Castilho, Miguel, LS Equine Muscoskeletal Biology, dES RMSC, Kristen, Marleen, Ainsworth, Madison J, Chirico, Nino, van der Ven, Casper F T, Doevendans, Pieter A, Sluijter, Joost P G, Malda, Jos, van Mil, Alain, and Castilho, Miguel

Published
2020

14. Fiber Scaffold Patterning for Mending Hearts: 3D Organization Bringing the Next Step

Author

Orthopaedie Onderzoek, Experimentele Afd. Cardiologie 2, Researchbureau DHL, Circulatory Health, Team Medisch, Regenerative Medicine and Stem Cells, Onderzoek Cardiovasculair Reg. Med., Team Onderzoek, Other research (not in main researchprogram), Kristen, Marleen, Ainsworth, Madison J., Chirico, Nino, van der Ven, Casper F.T., Doevendans, Pieter A., Sluijter, Joost P.G., Malda, Jos, van Mil, Alain, Castilho, Miguel, Orthopaedie Onderzoek, Experimentele Afd. Cardiologie 2, Researchbureau DHL, Circulatory Health, Team Medisch, Regenerative Medicine and Stem Cells, Onderzoek Cardiovasculair Reg. Med., Team Onderzoek, Other research (not in main researchprogram), Kristen, Marleen, Ainsworth, Madison J., Chirico, Nino, van der Ven, Casper F.T., Doevendans, Pieter A., Sluijter, Joost P.G., Malda, Jos, van Mil, Alain, and Castilho, Miguel

Published
2020

17. Fiber Scaffold Patterning for Mending Hearts: 3D Organization Bringing the Next Step.

Author

Kristen M, Ainsworth MJ, Chirico N, van der Ven CFT, Doevendans PA, Sluijter JPG, Malda J, van Mil A, and Castilho M

Subjects
Animals, Biocompatible Materials chemistry, Extracellular Matrix chemistry, Humans, Hydrogels chemistry, Myocardium cytology, Myocardium metabolism, Printing, Three-Dimensional, Heart Failure therapy, Tissue Engineering, Tissue Scaffolds chemistry
Abstract

Heart failure (HF) is a leading cause of death worldwide. The most common conditions that lead to HF are coronary artery disease, myocardial infarction, valve disorders, high blood pressure, and cardiomyopathy. Due to the limited regenerative capacity of the heart, the only curative therapy currently available is heart transplantation. Therefore, there is a great need for the development of novel regenerative strategies to repair the injured myocardium, replace damaged valves, and treat occluded coronary arteries. Recent advances in manufacturing technologies have resulted in the precise fabrication of 3D fiber scaffolds with high architectural control that can support and guide new tissue growth, opening exciting new avenues for repair of the human heart. This review discusses the recent advancements in the novel research field of fiber patterning manufacturing technologies for cardiac tissue engineering (cTE) and to what extent these technologies could meet the requirements of the highly organized and structured cardiac tissues. Additionally, future directions of these novel fiber patterning technologies, designs, and applicability to advance cTE are presented., (© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2020
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