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188 results on '"Ainla, Alar"'

2. Advances in Microfluidics for the Implementation of Liquid Biopsy in Clinical Routine

Author

Teixeira, Alexandra, Carneiro, Adriana, Piairo, Paulina, Xavier, Miguel, Ainla, Alar, Lopes, Cláudia, Sousa-Silva, Maria, Dias, Armando, Martins, Ana S., Rodrigues, Carolina, Pereira, Ricardo, Pires, Liliana R., Abalde-Cela, Sara, Diéguez, Lorena, Crusio, Wim E., Series Editor, Dong, Haidong, Series Editor, Radeke, Heinfried H., Series Editor, Rezaei, Nima, Series Editor, Steinlein, Ortrud, Series Editor, Xiao, Junjie, Series Editor, Caballero, David, editor, Kundu, Subhas C., editor, and Reis, Rui L., editor

Published
2022
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3. A sensorised, microfluidic Ussing chamber manufactured by stereolithography and soft lithography techniques

Author

Carnero Bastián, Radziunas-Salinas Yago, Rodiño-Janeiro Bruno K., Rodrigues Patrícia M., Ainla Alar, Xavier Miguel, Diéguez Lorena, and Flores-Arias María Teresa

Subjects
Physics, QC1-999
Abstract

Motivated by the need for improved platforms in biomedical research, this study addresses challenges associated with traditional Ussing chamber systems, widely used in studying biological barriers like the epithelial barrier of the gut. These challenges include complexity, high sample volumes, and limited compatibility. By combining stereolithography and soft lithography techniques, a microfluidic Ussing chamber is manufactured, overcoming these limitations, and incorporating compatibility with microscopy. Validation through Trans-Epithelial Electrical Resistance (TEER) measurements confirms its efficacy in assessing ion permeability dynamics, utilizing Caco-2 cell monolayers. The study showcases the capability of the manufactured chamber for sensing impact of calcium on tight junctions.

Published
2024
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5. Advances in Microfluidics for the Implementation of Liquid Biopsy in Clinical Routine

Author

Teixeira, Alexandra, primary, Carneiro, Adriana, additional, Piairo, Paulina, additional, Xavier, Miguel, additional, Ainla, Alar, additional, Lopes, Cláudia, additional, Sousa-Silva, Maria, additional, Dias, Armando, additional, Martins, Ana S., additional, Rodrigues, Carolina, additional, Pereira, Ricardo, additional, Pires, Liliana R., additional, Abalde-Cela, Sara, additional, and Diéguez, Lorena, additional

Published
2022
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6. Nanofluidic resistive pulse sensing for characterization of extracellular vesicles.

Author

Calado, Madalena R. C., Lage, Teresa C., André, Daniel A. M., Calaza, Carlos, Marques, Carlos, Herrero, Carolina, Piteira, João, Montelius, Lars, Petrovykh, Dmitri Y., Diéguez, Lorena, and Ainla, Alar

Subjects
EXTRACELLULAR vesicles, HUMAN cell culture, VESICLES (Cytology)
Abstract

This paper describes the development, design and characterization of a resistive pulse sensing (RPS) system for the analysis of size distributions of extracellular vesicles (EVs). The system is based on microfluidic chips fabricated using soft-lithography and operated in pressure-driven mode. This fabrication approach provided reproducible pore dimensions and the best performing chip design enabled, without calibration, sizing of both 252 nm and 460 nm test particles within 8% of theoretically calculated values, based on the size specifications provided by suppliers. The number concentration measurement had higher variations and without calibration provided estimates within an order of magnitude, for sample concentrations across 4 orders of magnitude. The RPS chips could also measure successfully EVs and other biological nanoparticles in purified samples from cell culture media and human serum. A compact, fast and inexpensive RPS system based on this design could be an attractive alternative to current gold-standard techniques for routine characterization of EV samples. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Flexible Pressure and Temperature Microsensors for Textile-Integrated Wearables

Author

dos Santos, Dimitri Emmanuel, primary, Queiroz, José Bento, additional, Garcia, Inês Sofia, additional, Vieira, João, additional, Fernandes, José, additional, Sotgiu, Edoardo, additional, Minas, Graça, additional, Bouçanova, Maria, additional, Arruda, Luisa Mendes, additional, Fangueiro, Raul, additional, Salgueiro-Oliveira, Anabela, additional, Ainla, Alar, additional, Serra Alves, Filipe, additional, and Alves Dias, Rosana, additional

Published
2024
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9. Development of Smart Clothing to Prevent Pressure Injuries in Bedridden Persons and/or with Severely Impaired Mobility: 4NoPressure Research Protocol

Author

Rêgo, Anderson da Silva, primary, Furtado, Guilherme Eustáquio, additional, Bernardes, Rafael A., additional, Santos-Costa, Paulo, additional, Dias, Rosana A., additional, Alves, Filipe S., additional, Ainla, Alar, additional, Arruda, Luisa M., additional, Moreira, Inês P., additional, Bessa, João, additional, Fangueiro, Raul, additional, Gomes, Fernanda, additional, Henriques, Mariana, additional, Sousa-Silva, Maria, additional, Pinto, Alexandra C., additional, Bouçanova, Maria, additional, Sousa, Vânia Isabel Fernande, additional, Tavares, Carlos José, additional, Barboza, Rochelne, additional, Carvalho, Miguel, additional, Filipe, Luísa, additional, Sousa, Liliana B., additional, Apóstolo, João A., additional, Parreira, Pedro, additional, and Salgueiro-Oliveira, Anabela, additional

Published
2023
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10. Abstract 4577: An innovative platform to mimic the tumoral vascular microenvironment (TME) of patients with metastatic colorectal cancer (mCRC) using bioprinted hydrogel microfluidics

Author

González, Nadia Saoudi, primary, Ballabrera, Francesc Salvà, additional, García, Ariadna, additional, Gonzalo-Ruiz, Javier, additional, Baraibar, Iosune, additional, Ros, Javier, additional, Rodriguez, Marta, additional, Díaz, María García, additional, Fusté, Marta Falcó, additional, Kashani, Melika Parchehbaf, additional, Honrado, Carlos, additional, Ainla, Alar, additional, Tabernero, Josep, additional, Diéguez, Lorena, additional, Fraiz, Elena Martínez, additional, and Élez, Elena, additional

Published
2023
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11. End-User Assessment of an Innovative Clothing-Based Sensor Developed for Pressure Injury Prevention: A Mixed-Method Study

Author

Rêgo, Anderson S., primary, Filipe, Luísa, additional, Dias, Rosana A., additional, Alves, Filipe S., additional, Queiroz, José, additional, Ainla, Alar, additional, Arruda, Luísa M., additional, Fangueiro, Raul, additional, Bouçanova, Maria, additional, Bernardes, Rafael A., additional, de Sousa, Liliana B., additional, Santos-Costa, Paulo, additional, Apóstolo, João A., additional, Parreira, Pedro, additional, and Salgueiro-Oliveira, Anabela, additional

Published
2023
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14. Autocatalytic, bistable, oscillatory networks of biologically relevant organic reactions

Author

Semenov, Sergey N., Kraft, Lewis J., Ainla, Alar, Zhao, Mengxia, Baghbanzadeh, Mostafa, Campbell, Victoria E., Kang, Kyungtae, Fox, Jerome M., and Whitesides, George M.

Subjects
Organic compounds -- Chemical properties, Chemical reactions -- Observations, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Sergey N. Semenov [1]; Lewis J. Kraft [1]; Alar Ainla [1]; Mengxia Zhao [1]; Mostafa Baghbanzadeh [1]; Victoria E. Campbell [1]; Kyungtae Kang [1]; Jerome M. Fox [1]; George [...]

Published
2016
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15. Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics

Author

Toldrà Filella, Anna, Ainla, Alar, Khaliliazar, Shirin, Landin, Roman, Chondrogiannis, Georgios, Hanze, Martin, Reu, Pedro, Hamedi, Mahiar, Toldrà Filella, Anna, Ainla, Alar, Khaliliazar, Shirin, Landin, Roman, Chondrogiannis, Georgios, Hanze, Martin, Reu, Pedro, and Hamedi, Mahiar

Abstract

The realization of electrochemical nucleic acid amplification tests (NAATs) at the point of care (POC) is highly desirable, but it remains a challenge given their high cost and lack of true portability/miniaturization. Here we show that mass-produced, industrial standardized, printed circuit boards (PCBs) can be repurposed to act as near-zero cost electrodes for self-assembled monolayer-based DNA biosensing, and further integration with a custom-designed and low-cost portable potentiostat. To show the analytical capability of this system, we developed a NAAT using isothermal recombinase polymerase amplification, bypassing the need of thermal cyclers, followed by an electrochemical readout relying on a sandwich hybridization assay. We used our sensor and device for analytical detection of the toxic microalgae Ostreopsis cf. ovata as a proof of concept. This work shows the potential of PCBs and open-source electronics to be used as powerful POC DNA biosensors at a low-cost., QC 20230523

Published
2022
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18. A microfluidic pipette for single-cell pharmacology

Author

Ainla, Alar, Jansson, Erik T., Stepanyants, Natalia, Orwar, Owe, and Jesorka, Aldo

Subjects
Microfluidics -- Research, Pharmacology -- Methods, Pharmacology -- Equipment and supplies, Pharmacology -- Technology application, Technology application, Chemistry
Abstract

We report on a free-standing microfluidic pipette made in poly(dimethylsiloxane) having a circulating liquid tip that generates a self-confining volume in front of the outlet channels. The method is flexible and scalable as the geometry and the size of the recirculation zone is defined by pressure, channel number, and geometry. The pipette is capable of carrying out a variety of complex fluid processing operations, such as mixing, multiplexing, or gradient generation at selected cells in cell and issue cultures. Using an uptake assay, we show that it is possible to generate dose--response curves in situ from adherent Chinese hamster ovary cells expressing proton-activated human transient receptor potential vanilloid (hTRPV1) receptors. Using confined superfusion and cell stimulation, we could activate hTRPVl receptors in single cells, measure the response by a patch-clamp pipette, and induce membrane bleb formation by exposing selected groups of cells to formaldehyde/dithiothreitol-containing solutions, respectively. In short, the microfluidic pipette allows for complex, contamination-free multiple-compound delivery for pharmacological screening of intact adherent cells. 10.1021/ac100480f

Published
2010

19. A microfluidic diluter based on pulse width flow modulation

Author

Ainla, Alar, Gozen, Irep, Orwar, Owe, and Jesorka, Aldo

Subjects
Microfluidics -- Research, Chemistry
Abstract

We demonstrate that pulse width flow modulation (PWFM) can be used to design fast, accurate, and precise multi-stage dilution modules for microfluidic devices. The PWFM stage unit presented here yields 10-fold dilution, but several PWFM stages can be connected in series to yield higher-order dilutions. We have combined two stages in a device thus capable of diluting up to 100-fold, and we have experimentally determined a set of rules that can be conveniently utilized to design multistage diluters. Microfabrication with resist-based molds yielded geometrical channel height variances of 7% (22.9(16) [micro]m) with corresponding hydraulic resistance variances of ~20%. Pulsing frequencies, channel lengths, and flow pressures can be chosen within a wide range to establish the desired diluter properties. Finally, we illustrate the benefits of on-chip dilution in an example application where we investigate the effect of the [Ca.sup.2+] concentration on a phospholipid bilayer spreading from a membrane reservoir onto a Si[O.sub.2] surface. This is one of many possible applications where flexible concentration control is desirable.

Published
2009

28. Molecular Lipid Films on Microengineering Materials

Author

Jõemetsa, Silver, primary, Spustova, Karolina, additional, Kustanovich, Kiryl, additional, Ainla, Alar, additional, Schindler, Severin, additional, Eigler, Siegfried, additional, Lobovkina, Tatsiana, additional, Lara-Avila, Samuel, additional, Jesorka, Aldo, additional, and Gözen, Irep, additional

Published
2019
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31. Fabricating 3D Structures by Combining 2D Printing and Relaxation of Strain

Author

Cafferty, Brian J., primary, Campbell, Victoria E., additional, Rothemund, Philipp, additional, Preston, Daniel J., additional, Ainla, Alar, additional, Fulleringer, Nicolas, additional, Diaz, Alina C., additional, Fuentes, Alberto E., additional, Sameoto, Dan, additional, Lewis, Jennifer A., additional, and Whitesides, George M., additional

Published
2018
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33. A Soft Tube-Climbing Robot

Author

Verma, Mohit S., primary, Ainla, Alar, additional, Yang, Dian, additional, Harburg, Daniel, additional, and Whitesides, George M., additional

Published
2018
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36. Ion sensing with thread-based potentiometric electrodes

Author

Mousavi, Maral P. S., primary, Ainla, Alar, additional, Tan, Edward K. W., additional, K. Abd El-Rahman, Mohamed, additional, Yoshida, Yumi, additional, Yuan, Li, additional, Sigurslid, Haakon H., additional, Arkan, Nooralhuda, additional, Yip, Mighten C., additional, Abrahamsson, Christoffer K., additional, Homer-Vanniasinkam, Shervanthi, additional, and Whitesides, George M., additional

Published
2018
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38. Arthrobots

Author

Nemiroski, Alex, primary, Shevchenko, Yanina Y., additional, Stokes, Adam A., additional, Unal, Baris, additional, Ainla, Alar, additional, Albert, Sahradha, additional, Compton, Gabrielle, additional, MacDonald, Emily, additional, Schwab, Yosyp, additional, Zellhofer, Caroline, additional, and Whitesides, George M., additional

Published
2017
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