48 results on '"Aibing WU"'
Search Results
2. Intelligent Algorithm Optimization of Liquid Manure Spreading Control
- Author
-
Pengjun Wang, Yongsheng Chen, Binxing Xu, Aibing Wu, Jingjing Fu, Mingjiang Chen, and Biao Ma
- Subjects
precision agriculture ,fertile field machinery ,flow control ,application of liquid manure ,GA-BPNN-PID control ,Agriculture (General) ,S1-972 - Abstract
The growth of field crops needs appropriate soil nutrients. As a basic fertilizer, liquid manure provides biological nutrients for crop growth and increases the content of organic matter in crops. However, improper spraying not only reduces soil fertility but also destroys soil structure. Therefore, the precise control of the amount of liquid manure is of great significance for agricultural production and weight loss. In this study, we first built the model of spraying control, then optimized the BP neural network algorithm through a genetic algorithm. The stability and efficiency of the optimized controller were compared with PID, fuzzy PID and BPNN-PID control. The simulation results show that the optimized algorithm has the shortest response time and lowest relative error. Finally, platform experiments were designed to verify the four control algorithms at four different vehicle speeds. The results show that, compared with other control algorithms, the control algorithm described here has good stability, short response time, small overshoot, and can achieve an accurate fertilizer application effect, providing an optimization scheme for research on the precise application of liquid manure.
- Published
- 2023
- Full Text
- View/download PDF
3. Working Mechanism and Parameter Optimization of a Crushing and Impurity Removal Device for Liquid Manure
- Author
-
Biao Ma, Mingjiang Chen, Aibing Wu, Jingjing Fu, Zhichao Hu, and Binxing Xu
- Subjects
liquid manure ,straw ,crushing and impurity removal device ,cross rotary cutter group ,blade ,Agriculture (General) ,S1-972 - Abstract
Aiming to solve the problems of easy clogging and high energy consumption of multi-way fertilization devices for liquid manure, a crushing and impurity removal device for liquid manure was designed by combining the physical characteristics of liquid manure and impurities, and building the corresponding test bench. The proposed device could crush flexible impurities such as straw and filoplume and intercept hard impurities with high density. The main structural parameters of the device were determined according to the survey analysis and the theoretical design. The influences of cutter head shape, cutter edge angle, cutter shaft speed, and cutting clearance on the disqualification rate and energy consumption of straw crushing were obtained by a single-factor experiment. Furthermore, the Box–Behnken central composite design method of the response surface was employed to investigate the effects of the cutter shaft speed, cutting clearance, and cutter edge angle on the disqualification rate and energy consumption of straw crushing. In addition, the working parameters of the device were optimized by employing the response surface method. On this basis, the mathematical relationship model among the disqualification rate, energy consumption, and all influencing factors was established. The results show that the optimal combination of working parameters includes a cutter shaft speed of 312 r/min, a cutting clearance of 1.4 mm, and a cutter edge angle of 45°. From the prediction model, the predicted failure rate was 4.15%, and the predicted energy consumption was 47.53 J. The verification experiment was then performed under the optimal combination of working parameters. The obtained disqualification rate was 4.08% and the energy consumption was 47.56 J, which met the design and work requirements.
- Published
- 2022
- Full Text
- View/download PDF
4. LINC00472 Acts as a Tumor Suppressor in NSCLC through KLLN-Mediated p53-Signaling Pathway via MicroRNA-149-3p and MicroRNA-4270
- Author
-
Aimei Zou, Xingli Liu, Zongjiong Mai, Junke Zhang, Zhuohuan Liu, Qilu Huang, Aibing Wu, and Chenyu Zhou
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Long non-coding RNAs and microRNAs (miRNAs) have been reported to participate in the progression of non-small-cell lung cancer (NSCLC). Long intergenic non-protein-coding RNA 472 (LINC00472), miR-149-3p, and miR-4270 were found to be involved in tumor activities, suggesting potential roles in NSCLC. Thus, this study aimed to examine the ability of LINC00472 to influence the progression of NSCLC with the involvement of miR-149-3p and miR-4270. Initially, differentially expressed long non-coding RNAs (lncRNAs), downstream regulatory miRNAs, and genes related to NSCLC were identified. Next, the interaction among LINC00472, miR-149-3p and miR-4270, and KLLN and the p53-signaling pathway was determined. The effect of LINC00472 on the expression of E-cadherin, N-cadherin, and Vimentin was examined through gain-of-function and loss-of-function experiments. Lastly, the effects of LINC00472 on NSCLC tumor growth were assessed in vivo. LINC00472 and KLLN were found to exhibit low levels, while miR-149-3p and miR-4270 were highly expressed in NSCLC. In addition, the overexpression of LINC00472 was observed to upregulate KLLN and activate the p53-signaling pathway, which ultimately inhibited the invasion, migration, and EMT of NSCLC cells via miR-149-3p and miR-4270, corresponding to decreased N-cadherin and Vimentin and increased E-cadherin. The overexpression of LINC00472 exerted an inhibitory effect on tumor growth in vivo. Taken together, the key evidence suggests that the overexpression of LINC00472 can downregulate miR-149-3p and miR-4270 to upregulate KLLN and activate the p53-signaling pathway, thus inhibiting the development of NSCLC. This study highlights the potential of LINC00472 as a promising therapeutic target for NSCLC treatment. Keywords: long intergenic non-protein-coding RNA 472, microRNA-149-3p, microRNA-4270, KLLN, p53-signaling pathway, non-small-cell lung cancer, invasion, migration, epithelial-mesenchymal transition
- Published
- 2019
- Full Text
- View/download PDF
5. CK2α Regulates the Metastases and Migration of Lung Adenocarcinoma A549 Cell Line through PI3K/Akt/GSK-3β Signal Pathway
- Author
-
Aibing WU, Mingchun LI, Zongjiong MAI, Shujun LI, and Zhixiong YANG
- Subjects
Lung neoplasms ,CK2α ,PI3K/Akt/GSK-3β signaling pathway ,Mesenchymal-to-epithelial transition ,Invasion ,Migration ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background and objective Lung cancer is the leading cancer-related death worldwide. Patients with lung cancer mainly died of tumor metastasis and invasion. Protein kinase CK2 is an ubiquitous serine/threonine protein kinase and is frequently upregulated in various human tumors. This study aims to explore the effect and molecular mechanism of the invasion and migration of lung adenocarcinoma A549 cells after knock-down of CK2α expression. Methods The pSilencerTM 4.1-siCK2α-eGFP of lentiviral-mediated shRNA was constructed. The expression of CK2α was knock-downed, and a stable A549 cell line was established. The invasion and migration of A549 cell line was detected through Transwell and Boyden chamber assays. The protein expression of the PI3K/Akt signaling pathway and mesenchymal-to-epithelial transition (EMT) was evaluated using Western blot analysis. Results The invasion and migration of A549 cells were significantly inhibited after the knockdown of CK2α expression compared with that in the control group. p-PTEN, Akt, p-Akt473, p-Akt308, p-PDK1, p-c-Raf, and p-GSK-3β were significantly downregulated, whereas PTEN was upregulated. Moreover, vimentin, β-catenin, Snail, MMP2, and MMP9 were significantly downregulated after reducing the CK2α expression. Conclusion CK2α might regulate the invasion and migration of A549 cells through the PI3K/Akt/GSK-3β/Snail signaling pathway, which controls EMT in lung adenocarcinoma.
- Published
- 2017
- Full Text
- View/download PDF
6. MiR-373-3p Promotes Invasion and Metastasis of Lung Adenocarcinoma Cells
- Author
-
Aibing WU, Jinmei LI, Kunpeng WU, Yanli MO, Yiping LUO, Haiyin YE, Xiang SHEN, Shujun LI, Yahai LIANG, Meilian LIU, and Zhixiong YANG
- Subjects
Lung neoplasms ,MiR-373 ,MMP-9 ,MMP-14 ,Invasion ,Metastasis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background and objective Lung cancer is the leading cause of cancer-related deaths worldwide, and metastasis is the major cause of death in lung cancer patients. MiR-373 is closely associated with invasion and metastasis in other tumor cells. This study explored the expression of miR-373-3p in non-small cell lung cancer (NSCLC) and its effect on the invasive and metastatic capabilities of lung adenocarcinoma cells, as well as their mechanisms of action. Methods The expression of miR-373-3p in NSCLC tissues and lung adenocarcinoma cell lines was detected by quantitative reverse transcription polymerase chain reaction. The roles of miR-373-3p in regulating lung adenocarcinoma cell invasion and metastatic properties were analyzed with miR-373-3p mimic/inhibitor-transfected cells via Transwell chamber assay. Matrix metalloproteinase MMP-9 and MMP-14 protein levels were detected by Western blot in lung cancer cells after transfection. Results MiR-373-3p was upregulated in 51 NSCLC tissues and 5 NSCLC cell lines. Gain-of-function and loss-of-function studies showed that overexpression of miR-373-3p promoted H1299 cell migration and invasion, which resulted in upregulation of MMP-9 and MMP-14. By contrast, miR-373-3p knockdown inhibited these processes in A549 cells and downregulated the expression of MMP-9 and MMP-14. Conclusion Our results demonstrated that miR-373-3p participated in the invasion and metastasis of lung adenocarcinoma cells, partly by upregulation of MMP-9 and MMP-14.
- Published
- 2015
- Full Text
- View/download PDF
7. ZEB2 mediates multiple pathways regulating cell proliferation, migration, invasion, and apoptosis in glioma.
- Author
-
Songtao Qi, Ye Song, Yuping Peng, Hao Wang, Hao Long, Xiaoli Yu, Zhiyong Li, Luxiong Fang, Aibing Wu, Weiren Luo, Yan Zhen, Ying Zhou, Yan Chen, Chunping Mai, Zhen Liu, and Weiyi Fang
- Subjects
Medicine ,Science - Abstract
BackgroundThe aim of the present study was to analyze the expression of Zinc finger E-box Binding homeobox 2 (ZEB2) in glioma and to explore the molecular mechanisms of ZEB2 that regulate cell proliferation, migration, invasion, and apoptosis.Methodology/principal findingsExpression of ZEB2 in 90 clinicopathologically characterized glioma patients was analyzed by immunohistochemistry. Furthermore, siRNA targeting ZEB2 was transfected into U251 and U87 glioma cell lines in vitro and proliferation, migration, invasion, and apoptosis were examined separately by MTT assay, Transwell chamber assay, flow cytometry, and western blot.ResultsThe expression level of ZEB2 protein was significantly increased in glioma tissues compared to normal brain tissues (PConclusionOverexpression of ZEB2 is an unfavorable factor that may facilitate glioma progression. Knockdown ZEB2 expression by siRNA suppressed cell proliferation, migration, invasion and promoted cell apoptosis in glioma cells.
- Published
- 2012
- Full Text
- View/download PDF
8. Discrete element modeling and physical experiment research on the biomechanical properties of cotton stalk.
- Author
-
Weisong Zhao, Mingjiang Chen, Jianhua Xie, Silin Cao, Aibing Wu, and Zhenwei Wang
- Published
- 2023
- Full Text
- View/download PDF
9. Mettl3-Mediated M6a Modification Plays a Role in Lipid Metabolism Disorders and Progressive Liver Damage in Mice by Regulating Lipid Metabolism-Related Gene Expression
- Author
-
Guanqi Dai, Yonglong Li, Shihao Huang, Xueyi Tu, Zhihao Zhou, Wanyi Chen, Jintao Lin, Yingchun Li, Danhua He, Taoyan Lin, Jinge Cong, Ye Lei, Jiawei Xia, Liuxin Han, Zhenxia Yao, Weiwei Liu, Ao Zhang, Ying Zhou, Qiwen Li, Jing Li, Aibing Wu, Dong Xiao, Junshuang Jia, Wentao Zhao, and Xiaolin Lin
- Published
- 2023
10. Activation of the Hedgehog pathway mediates resistance to epidermal growth factor receptor inhibitors in non-small cell lung cancer
- Author
-
Hualin, Chen, Donghong, Yang, Yongcun, Wang, Hua, Tao, Yiping, Luo, Aibing, Wu, Shujun, Li, Zhixiong, Yang, and Ming, Chen
- Subjects
Oncology - Abstract
The current study aimed to investigate the function of the Hedgehog pathway and its association with epithelial-mesenchymal transition (EMT) in epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) resistance in non-small cell lung cancer (NSCLC). Lung tumor tissue specimens from EGFR TKI-resistant patients, including those with brain metastases, had hyperactive Hedgehog signaling compared with those from TKI-sensitive patients. SHH stimulation promoted GLI1 activation as well as cell motility in parental PC9 cells while suppressing gefitinib-induced apoptosis in gefitinib-resistant cells. SHH also promoted EMT in parental PC9 cells via E-cadherin suppression and N-cadherin and vimentin upregulation. The knockdown of GLI1 exhibited the opposite effects. Besides, SHH induced, whereas GLI1 knockdown reversed gefitinib resistance in xenograft tumors. The Hedgehog pathway inhibitor GDC-0449 synergized with gefitinib to increase xenograft tumor sensitivity to chemotherapy and extend survival in tumor-bearing animals. These results suggest the Hedgehog pathway mediates EGFR TKI resistance and induces EMT in NSCLC, representing a potential therapeutic target to defeat TKI resistance.
- Published
- 2022
11. Discrete Element Modelling of Cotton Stalk and its Verification
- Author
-
Weisong Zhao, Chen Mingjiang, Jianhua Xie, Aibing Wu, and Wang Zhenwei
- Published
- 2022
12. RETRACTED: LINC00472 Acts as a Tumor Suppressor in NSCLC through KLLN-Mediated p53-Signaling Pathway via MicroRNA-149-3p and MicroRNA-4270
- Author
-
Xingli Liu, Zhuohuan Liu, Chenyu Zhou, Zongjiong Mai, Junke Zhang, Qilu Huang, Aimei Zou, and Aibing Wu
- Subjects
microRNA-4270 ,0301 basic medicine ,epithelial-mesenchymal transition ,p53-signaling pathway ,Vimentin ,migration ,Article ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,law ,Drug Discovery ,microRNA ,medicine ,KLLN ,Epithelial–mesenchymal transition ,Lung cancer ,Gene ,biology ,lcsh:RM1-950 ,long intergenic non-protein-coding RNA 472 ,RNA ,microRNA-149-3p ,invasion ,medicine.disease ,respiratory tract diseases ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,non-small-cell lung cancer ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,Molecular Medicine ,Suppressor - Abstract
Long non-coding RNAs and microRNAs (miRNAs) have been reported to participate in the progression of non-small-cell lung cancer (NSCLC). Long intergenic non-protein-coding RNA 472 (LINC00472), miR-149-3p, and miR-4270 were found to be involved in tumor activities, suggesting potential roles in NSCLC. Thus, this study aimed to examine the ability of LINC00472 to influence the progression of NSCLC with the involvement of miR-149-3p and miR-4270. Initially, differentially expressed long non-coding RNAs (lncRNAs), downstream regulatory miRNAs, and genes related to NSCLC were identified. Next, the interaction among LINC00472, miR-149-3p and miR-4270, and KLLN and the p53-signaling pathway was determined. The effect of LINC00472 on the expression of E-cadherin, N-cadherin, and Vimentin was examined through gain-of-function and loss-of-function experiments. Lastly, the effects of LINC00472 on NSCLC tumor growth were assessed in vivo. LINC00472 and KLLN were found to exhibit low levels, while miR-149-3p and miR-4270 were highly expressed in NSCLC. In addition, the overexpression of LINC00472 was observed to upregulate KLLN and activate the p53-signaling pathway, which ultimately inhibited the invasion, migration, and EMT of NSCLC cells via miR-149-3p and miR-4270, corresponding to decreased N-cadherin and Vimentin and increased E-cadherin. The overexpression of LINC00472 exerted an inhibitory effect on tumor growth in vivo. Taken together, the key evidence suggests that the overexpression of LINC00472 can downregulate miR-149-3p and miR-4270 to upregulate KLLN and activate the p53-signaling pathway, thus inhibiting the development of NSCLC. This study highlights the potential of LINC00472 as a promising therapeutic target for NSCLC treatment. Keywords: long intergenic non-protein-coding RNA 472, microRNA-149-3p, microRNA-4270, KLLN, p53-signaling pathway, non-small-cell lung cancer, invasion, migration, epithelial-mesenchymal transition
- Published
- 2019
13. NEAT1 is a potential prognostic biomarker for patients with nasopharyngeal carcinoma
- Author
-
Jian Wu, Zhixiong Yang, Dan Tian, Zhuoxing Liu, Kunpeng Wu, Aibing Wu, and Yue Chen
- Subjects
Adult ,Male ,0301 basic medicine ,Oncology ,Prognostic factor ,medicine.medical_specialty ,Biochemistry ,Disease-Free Survival ,03 medical and health sciences ,0302 clinical medicine ,Meta-Analysis as Topic ,Negatively associated ,Internal medicine ,Biomarkers, Tumor ,otorhinolaryngologic diseases ,medicine ,Humans ,Prognostic biomarker ,In patient ,RNA, Neoplasm ,Molecular Biology ,Aged ,Nasopharyngeal Carcinoma ,business.industry ,Proportional hazards model ,Nasopharyngeal Neoplasms ,Cell Biology ,Middle Aged ,medicine.disease ,Confidence interval ,Gene Expression Regulation, Neoplastic ,Survival Rate ,stomatognathic diseases ,030104 developmental biology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Biomarker (medicine) ,Female ,RNA, Long Noncoding ,business - Abstract
Nuclear paraspeckle assembly transcript 1 (NEAT1) has been found to be dysregulated and associated with clinical progression in various human cancers. The clinical and prognostic value of NEAT1 in nasopharyngeal carcinoma (NPC) was still controversial. The aim of our study was to provide more sufficient evidence that NEAT1 expression is correlated with overall survival in patients with NPC. NEAT1 expression was detected in NPC tissue samples, and the relationship between NEAT1 expression and clinical parameters, including prognosis, was analyzed. The meta-analysis was performed to further assess the prognostic significance of NEAT1 expression in patients with NPC. In our study, we found that the levels of NEAT1 expression were increased in NPC clinical tissue specimens, and associated with advanced M classification and clinical stages. Moreover, the Kaplan-Meier analysis suggested that the levels of NEAT1 expression were negatively associated with the overall survival of patients with NPC. Furthermore, univariate and multivariate Cox regression analyses showed that NEAT1 high-expression was an independent unfavorable prognostic factor in patients with NPC. Finally, we conducted a meta-analysis including 297 patients with NPC from the three studies, and found the pooled HR (95% confidence interval [CI]) was 1.64 (95% CI: 0.68-3.93) for the random effects model and 2.04 (95% CI: 1.42-2.95) for the fixed effect model. In conclusion, NEAT1 is a potential prognostic biomarker for NPC, but more studies are needed to further verify the prognostic value of NEAT1 in patients with NPC.
- Published
- 2019
14. Fluorescent and magnetic nanocomposites of Y2O3:Eu3+, Dy3+ and Fe or Fe3O4
- Author
-
Wenwu Zhao, Bin Hao, and Aibing Wu
- Subjects
Nanocomposite ,Materials science ,Magnetism ,Nanoparticle ,Bioengineering ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Microstructure ,01 natural sciences ,Fluorescence ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Modeling and Simulation ,Magnetic components ,General Materials Science ,Nanorod ,0210 nano-technology ,Bifunctional - Abstract
Eu3+ and Dy3+ codoped Y2O3 (Y2O3:Eu3+, Dy3+) based bifunctional nanocomposites were fabricated by solid-state route. The magnetic component of the nanocomposites is Fe or Fe3O4. The composition and microstructure of the nanocomposites were studied. The results indicate that nanocomposites are mainly consisted of Y2O3:Eu3+, Dy3+ nanorods and Fe (or Fe3O4) nanoparticles. The length and diameter of the nanorods are in the size range of 500–1500 nm and 80–150 nm, respectively. The size of Fe or Fe3O4 nanoparticles is about 20 nm. A probable formation mechanism of nanocomposites has been analyzed. The fluorescence and magnetism of nanocomposites have been evaluated based on the influences of amount of raw materials.
- Published
- 2021
15. [Role of hydrogen sulfide on expression of phosphatidylinositol 3 kinase/protein kinase B signal pathway in rats with intestinal ischemia/reperfusion injury]
- Author
-
Genlin, Lu, Aibing, Wu, and Hongbin, Wang
- Subjects
Male ,Phosphatidylinositol 3-Kinases ,Reperfusion Injury ,Animals ,Hydrogen Sulfide ,Phosphatidylinositol 3-Kinase ,Rats, Wistar ,Proto-Oncogene Proteins c-akt ,Rats ,Signal Transduction - Abstract
To explore the effect of hydrogen sulfide (HThirty male Wistar rats were divided into sham operation group (Sham group), IRI group, and HCompared with the Sham group, there was intestinal mucosa structure disorder edema and shedding villous fracture in the IRI group. Ileal pathological score in IRI group was significantly increased (4.21±0.15 vs. 0.15±0.03, P0.01), while plasma HH
- Published
- 2020
16. A comparative study on etoposide combined with lobaplatin or cisplatin in the first-line treatment of extensive-stage small cell lung cancer
- Author
-
Shujun, Li, Yahai, Liang, Yanxia, Wu, Zhong, Huang, Yanming, Lin, Zhixiong, Yang, Hualin, Chen, and Aibing, Wu
- Subjects
Adult ,Male ,Lung Neoplasms ,Organoplatinum Compounds ,Middle Aged ,Small Cell Lung Carcinoma ,Disease-Free Survival ,Antineoplastic Combined Chemotherapy Protocols ,Biomarkers, Tumor ,Humans ,Female ,Cisplatin ,Cyclobutanes ,Aged ,Epirubicin ,Etoposide - Abstract
To compare the efficacy and safety of etoposide combined with lobaplatin or cisplatin in the first-line treatment of extensive-stage small cell lung cancer (SCLC).A total of 98 extensive-stage SCLC patients treated at the Oncology Department from March 2015 to March 2017 were enrolled and divided into etoposide + lobaplatin group (EL group, n=49) and etoposide + cisplatin group (EP group, n=49) using a random number table. The clinical data of all patients were collected, and the short-term effective rate, changes in the levels of serum tumor markers carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1) and neurone specific enolase (NSE) before and after chemotherapy and adverse reactions were compared between the two groups. Moreover, the patients were followed up, and the overall survival (OS) and progression-free survival (PFS) were recorded.In EL group and EP group, the level of serum NSE significantly declined after treatment compared with that before treatment, but the levels of serum CEA and CYFRA21-1 were not significantly decreased after chemotherapy compared with those before chemotherapy. The incidence rate of leukopenia, erythropenia and thrombocytopenia was 71.4%, 44.9% and 40.8%, respectively, in EL group, and 85.7%, 30.6% and 24.5%, respectively, in EP group, and the degree I-II decline was more common in both groups. The proportion of gastrointestinal reactions was 14.3% and 59.2%, respectively, in EL group and EP group, with significant difference between the two groups. During follow-up, the 1-year OS was 59.2% (29/49) and 51.9% (25/49), respectively, and the 2-year OS was 26.5% (13/49) and 20.4% (10/49), respectively, in EL group and EP group. The survival curves of were plotted using the Kaplan-Meier method and log-rank test showed no statistically significant differences in the OS and PFS between the two groups.The short-term efficacy of EL and EP regimens is equivalent in the first-line treatment of extensive-stage SCLC, both OS and PFS are similar, and the adverse reactions can be tolerated. The EL regimen produced mild gastrointestinal reactions, and is worthy of clinical popularization.
- Published
- 2020
17. A primary plus secondary local PWHT method for mitigating weld residual stresses in pressure vessels
- Author
-
Wang Jinguang, Qiang Jin, Shan-Tung Tu, Xiaodong Pan, Wenbin Gu, Aibing Wu, Kai Zhang, Gang Li, Ming Song, and Wenchun Jiang
- Subjects
0209 industrial biotechnology ,Piping ,Materials science ,business.industry ,Mechanical Engineering ,technology, industry, and agriculture ,02 engineering and technology ,Welding ,Structural engineering ,Pressure vessel ,Finite element method ,law.invention ,020303 mechanical engineering & transports ,020901 industrial engineering & automation ,Electricity generation ,0203 mechanical engineering ,Mechanics of Materials ,law ,Residual stress ,General Materials Science ,business ,Large diameter - Abstract
Local post-weld heat treatment (PWHT) is often used for mitigating welding-induced residual stresses in pressure vessels and piping systems. As large and ultra-large pressure vessels are increasingly used in petrochemical and power generation industries, traditional local PWHT procedures stipulated in Codes and Standards become difficult to implement for some of the ultra-large vessels due to their very large diameter and overall length. In this paper, a new method referred to as primary-secondary heating based local PWHT technique (PS-PWHT) is proposed. Both finite element residual stress modeling and experimental residual stress measurements were performed for both validating the effectiveness of the proposed technique and elucidating the underling mechanisms. The results show that the PS-PWHT method can reduce welding-induced residual stresses in a significant manner, even leading to some level of compressive residual stresses at the vessel weld region.
- Published
- 2021
18. Concurrent versus sequential whole brain radiotherapy and TKI in EGFR-mutated NSCLC patients with brain metastasis: A single institution retrospective analysis
- Author
-
Yiping Luo, Ming Chen, Aibing Wu, Shujun Li, Zhixiong Yang, Donghong Yang, Hua Tao, and Hualin Chen
- Subjects
0301 basic medicine ,Oncology ,Male ,Lung Neoplasms ,medicine.medical_treatment ,0302 clinical medicine ,Epidermal growth factor ,Carcinoma, Non-Small-Cell Lung ,tyrosine kinase inhibitors ,brain metastasis ,Single institution ,Brain Neoplasms ,Whole brain radiotherapy ,Brain ,General Medicine ,Middle Aged ,Combined Modality Therapy ,ErbB Receptors ,Treatment Outcome ,030220 oncology & carcinogenesis ,whole brain radiotherapy ,Female ,Research Article ,medicine.medical_specialty ,Observational Study ,03 medical and health sciences ,Internal medicine ,medicine ,Carcinoma ,Humans ,Protein Kinase Inhibitors ,Survival analysis ,Aged ,Retrospective Studies ,business.industry ,Retrospective cohort study ,medicine.disease ,Survival Analysis ,respiratory tract diseases ,Radiation therapy ,030104 developmental biology ,Cranial Irradiation ,concurrent ,EGFR mutation ,business ,sequential ,Brain metastasis ,Follow-Up Studies ,nonsmall cell lung cancer - Abstract
To examine the outcomes of concurrent versus sequential whole-brain radiotherapy (WBRT) and epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) in nonsmall cell lung cancer (NSCLC) patients with EGFR mutation. Retrospectively 105 patients with NSCLC, brain metastasis, and EGFR mutation (Affiliated Hospital of Guangdong Medical University, 01/2011 to 12/2014) were grouped as: EGFR-TKIs alone (n = 39, group A), EGFR-TKIs + concurrent radiotherapy (n = 34, group B), and radiotherapy followed by EGFR-TKIs (n = 32, group C). The intracranial objective response rates of groups A, B, and C were 66.7%, 85.3%, and 75%, respectively (P .05). EGFR-TKIs and WBRT by simultaneous application improved the short- and long-term benefits to patients with NSCLC brain metastasis carrying EGFR mutation compared to concurrent application or EGFR-TKIs alone without additional adverse events.
- Published
- 2018
19. Photocatalytic Regeneration of Activated Carbon by Combining g-C3N4 Photocatalyst under Visible Light Irradiation
- Author
-
Xiuping Yue, Yifei Luo, Liang Zhao, Jian Zeng, Zhichao Liu, Tian Chen, Zeyu Du, Jianhui Shi, and Aibing Wu
- Subjects
Materials science ,Regeneration (biology) ,Visible light irradiation ,Photocatalysis ,medicine ,Photochemistry ,Electronic, Optical and Magnetic Materials ,Activated carbon ,medicine.drug - Published
- 2020
20. The effects of quartz content on the formation of residual water in a brine–CO2–quartz system: An experimental study
- Author
-
Qingchun Yu, Xufeng Li, Yi Li, Aibing Wu, and Cheng Zhang
- Subjects
geography ,geography.geographical_feature_category ,Capillary action ,Energy Engineering and Power Technology ,Mineralogy ,Aquifer ,Geotechnical Engineering and Engineering Geology ,Residual ,Supercritical fluid ,Permeability (earth sciences) ,Fuel Technology ,Brine ,Geotechnical engineering ,Drainage ,Quartz ,Geology - Abstract
Geo-sequestration of CO2 in deep saline aquifers is achieved by injecting CO2 into the aquifers and displacing brine. The residual water formed during the drainage process has a strong influence on traps of residual-gas. Moreover, in the context of CO2 geological storage, the characteristics of the brine–CO2–quartz system directly impact residual trapping capacities. We conducted experiments to investigate the influence of quartz content in the rocks on the formation of residual water and how much of this residual water remains after CO2 is injected. Three sandstone core samples were all saturated with 35 g/L NaCl brine. Supercritical CO2 was injected into the samples at aquifer temperature and pressure and the displaced water and water-gas mixtures were collected and measured. The results show that the irreducible water saturation was lower with higher quartz content in the rock core. The permeability of rock cores can only influence the drainage efficiency; it does not have a decisive impact on the irreducible water saturation. Based on drainage flow rates, the process of drainage can be divided into three stages termed: Pushing Drainage, Portable Drainage and Dissolved Drainage. This terminology differs slightly from previous time-frame characterizations. Note that we have used a capillary model to interpret the mechanisms that characterize the three stages in these experiments.
- Published
- 2015
21. SHCBP1 promotes cisplatin induced apoptosis resistance, migration and invasion through activating Wnt pathway
- Author
-
Xinfa Yu, Yan Lu, Chengyu Zhou, Aibing Wu, Meihua Luo, and Aimei Zou
- Subjects
0301 basic medicine ,Lung Neoplasms ,Antineoplastic Agents ,Apoptosis ,Adenocarcinoma ,030226 pharmacology & pharmacy ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Cell Movement ,medicine ,Humans ,Neoplasm Invasiveness ,General Pharmacology, Toxicology and Pharmaceutics ,Lung cancer ,Wnt Signaling Pathway ,Cells, Cultured ,beta Catenin ,Cell Proliferation ,Cisplatin ,Matrigel ,Gene knockdown ,Chemistry ,Wnt signaling pathway ,General Medicine ,Prognosis ,medicine.disease ,Apoptosis resistance ,Gene Expression Regulation, Neoplastic ,Survival Rate ,Wnt Proteins ,030104 developmental biology ,Shc Signaling Adaptor Proteins ,Drug Resistance, Neoplasm ,Carcinoma, Squamous Cell ,Disease Progression ,Cancer research ,medicine.drug - Abstract
Lung cancer is the leading cause for cancer death due to refractory nature to current treatment strategies, understanding the regulatory mechanism of therapy resistance of lung cancer is important for lung cancer therapy. Here, we aimed to study the role of SHCBP1 in lung cancer cisplatin resistance, we found SHCBP1 was upregulated in lung cancer tissues and cells, patients with high SHCBP1 had poor prognosis. SHC binding and spindle associated 1 (SHCBP1) overexpression promoted cisplatin induced apoptosis resistance, migration and invasion determined by apoptosis assay and transwell assay with or without Matrigel, while SHCBP1 knockdown inhibited cisplatin induced apoptosis resistance, migration and invasion. Wnt pathway promoted lung cancer progression, we found SHCBP1 activated Wnt pathway, characterized by promoting β-catenin nuclear translocation. Inhibition of Wnt pathway in SHCBP1 overexpression cells reversed the effect of SHCBP1 overexpression, confirming SHCBP1 promoted lung cancer progression through activating Wnt pathway. We also found SHCBP1 expression was positively corrected with Wnt pathway activity in lung cancer samples. In summary, we found SHCBP1 promoted cisplatin induced apoptosis resistance, migration and invasion through activating Wnt pathway, providing a potential target for lung cancer therapy.
- Published
- 2019
22. High-mobility group A2 overexpression is an unfavorable prognostic biomarker for nasopharyngeal carcinoma patients
- Author
-
Zhixiong Yang, Kunpeng Wu, Aibing Wu, and Zhuoxing Liu
- Subjects
Adult ,Male ,Epithelial-Mesenchymal Transition ,Clinical Biochemistry ,Nasopharyngeal neoplasm ,Young Adult ,HMGA2 ,Cell Line, Tumor ,Biomarkers, Tumor ,otorhinolaryngologic diseases ,medicine ,Humans ,RNA, Messenger ,Epithelial–mesenchymal transition ,Molecular Biology ,Aged ,Aged, 80 and over ,Gene knockdown ,Nasopharyngeal Carcinoma ,biology ,Microarray analysis techniques ,Carcinoma ,HMGA2 Protein ,Nasopharyngeal Neoplasms ,Cell Biology ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,Nasopharyngeal carcinoma ,Lymphatic Metastasis ,biology.protein ,Cancer research ,Biomarker (medicine) ,Immunohistochemistry ,Female - Abstract
Our previous study showed that knockdown of high-mobility group A2 (HMGA2) could suppress nasopharyngeal carcinoma (NPC) cell migration, invasion, and epithelial-mesenchymal transition (EMT) process, and HMGA2 is a direct functional target of let-7 to regulate NPC cell migration, invasion, and EMT process. However, little is known about the clinical and prognostic significance of HMGA2 protein in NPC patients. The purpose of this study is to identify the clinical and prognostic roles of HMGA2 in NPC patients. We initially analyzed the microarray data and verified mRNA and protein levels of HMGA2 in NPC tissues. Immunohistochemical staining for HMGA2 protein was performed in 116 NPC patients. The associations between HMGA2 protein expression and clinicopathologic features and its prognostic significance were analyzed. In our results, we found mRNA and protein expressions of HMGA2 were upregulated in NPC tissues and cell lines. In 116 NPC tissue samples, we observed that HMGA2 protein overexpression was associated with clinical stage, lymph node metastasis, and distant metastasis. Moreover, NPC patients with high levels of HMGA2 protein expression had shorter overall survival in comparison to patients with low levels of HMGA2 protein. In multivariate analysis, HMGA2 protein overexpression was an unfavorable prognostic factor for NPC patients. In conclusion, HMGA2 is an important biomarker to predicting NPC patient's survival time.
- Published
- 2015
23. Photocatalytic Regeneration of Activated Carbon by Combining g-C3N4 Photocatalyst under Visible Light Irradiation.
- Author
-
Jianhui Shi, Aibing Wu, Tian Chen, Zhichao Liu, Yifei Luo, Zeyu Du, Gang Cheng, and Xiuping Yue
- Published
- 2020
- Full Text
- View/download PDF
24. MMP-14 overexpression correlates with poor prognosis in non-small cell lung cancer
- Author
-
Aibing Wu, Bin Wu, Zhi-cheng Yang, Yan-li Mo, Zhixiong Yang, Yuzhou Wang, Jinmei Li, Kunpeng Wu, and Meng Xu
- Subjects
Adult ,Male ,Oncology ,medicine.medical_specialty ,Lung Neoplasms ,Kaplan-Meier Estimate ,Downregulation and upregulation ,Carcinoma, Non-Small-Cell Lung ,Internal medicine ,Biomarkers, Tumor ,Matrix Metalloproteinase 14 ,medicine ,Carcinoma ,Humans ,Stage (cooking) ,Lung cancer ,Aged ,Proportional Hazards Models ,business.industry ,Proportional hazards model ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Immunohistochemistry ,Up-Regulation ,Tumor progression ,Biomarker (medicine) ,Female ,business - Abstract
Matrix metalloproteinase-14 (MMP-14) has been demonstrated to play an important role in tumor progression. The aim of this study was to analyze the correlation between MMP-14 expression and clinicopathologic features and its prognostic significance in non-small cell lung cancer (NSCLC). Immunohistochemical staining for MMP-14 protein was performed in 104 patients with NSCLC. High levels of MMP-14 protein were positively correlated with the status of clinical stage (I-II vs. III-IV; P
- Published
- 2014
25. Evaluation of the Solid Digestate from Garage-type High Solids Anaerobic Digestion of Bundled Rice Straw and Swine Manure as a Growth Medium for Seeding Production.
- Author
-
Jingjing Fu, Biao Ma, Binxing Xu, Chunsong Guan, Aibing Wu, and Yongsheng Chen
- Subjects
SWINE manure ,SWINE growth ,WHEAT straw ,RICE straw ,SEED industry ,ANAEROBIC digestion ,SOLIDS - Abstract
The solid digestate from high solid anaerobic digestion was used as growth medium for seeding production. The garage-type dry fermentation system using bundled rice straw and swine manure was performed to obtain solid digestate. The addition of solid digestate addition greatly influenced the properties of the growth medium. The bulk density increased and the total porosity, pH, and electrical conductivity (EC) values were decreased with the reduction of solid digestate. The solid digestate-based media had a bulk density < 0.3 g/cm3, total porosity > 70%, air filled porosity ~ 3%, water holding porosity > 60%, EC < 3 mS/cm, and 6.5 < pH < 8. Those properties almost satisfied the essential requirements of nursery substrate. Also, the concentrations of nutrients and heavy metals of the substrate exhibited a positive relationship with solid digestate addition, and they are all within acceptable ranges for plant growth. When the addition of solid digestate was 50% (v/v), the germination rate of tomato seeding cultivated in that solid digestate-based growth medium reached 85%. These findings showed that the solid digestate from the high solid anaerobic digestion could be successfully applied in the seeding nursery and merit consideration for industrial applications. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
26. Aldehyde dehydrogenase 1, a functional marker for identifying cancer stem cells in human nasopharyngeal carcinoma
- Author
-
Zhixiong Yang, Gong Zhang, Aibing Wu, Qianbing Zhang, Kaitai Yao, Weiren Luo, and Si-Yi Li
- Subjects
Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Epithelial-Mesenchymal Transition ,Adolescent ,Biology ,Aldehyde Dehydrogenase 1 Family ,Kruppel-Like Factor 4 ,Mice ,SOX2 ,Cancer stem cell ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Epithelial–mesenchymal transition ,Aged ,Neoplasm Staging ,Nasopharyngeal Carcinoma ,Carcinoma ,Retinal Dehydrogenase ,Nasopharyngeal Neoplasms ,Middle Aged ,medicine.disease ,Isoenzymes ,Oncology ,Nasopharyngeal carcinoma ,KLF4 ,Cancer cell ,Neoplastic Stem Cells ,Cancer research ,Female ,Stem cell ,Clone (B-cell biology) ,Biomarkers - Abstract
Aldehyde dehydrogenase 1 (ALDH1) activity has now been employed successfully as a cancer stem cells (CSCs) marker in various tumors. We wanted to investigate whether ALDH1 can be used as a putative CSCs marker and a powerful prognostic factor in nasopharyngeal carcinoma (NPC). Here, we isolated ALDH1-positive cells from human NPC cell lines (5-8F and CNE2) and found that ALDH1-positive cancer cells grew faster and had higher clone formation efficiency (0.435 ± 0.15; 0.431 ± 0.025 vs. 0.131 ± 0.007; 0.121 ± 0.126), differentiation capability and had higher migration (233.00 ± 5.29; 228.60 ± 9.34 vs. 123.20 ± 7.70 vs. 97.20 ± 6.61) than those of ALDH1-negative cancer cells in vitro. In addition, ALDH1- positive cancer cells formed significantly more tumor spheres. Our in vivo experiments showed that only 5 × 103 ALDH1-positive NPC cells were required to induce tumors. Notably, ALDH1-positive cells are enriched in the side-population (SP) cells, and stem cells-like genes OCT4, BMI1, KLF4 and SOX2 are preferentially expressed in ALDH1-positive cells. Immunohistochemical results showed that the expression of ALDH1 correlated significantly with T classification (P = 0.011), N classification (P = 0.005), M classification (P = 0.002) and clinical stage (P = 0.001). Interestingly, ALDH1 expression in the tumor correlated significantly with epithelial–mesenchymal transition (EMT) markers including vimentin expression and loss of E-cadherin (P = 0.003 and 0.008, respectively). Furthermore, multivariate analyses showed that ALDH1 expression was an independent prognostic indicator (P = 0.032). Taken together, for the first time, we demonstrate that ALDH1 could be a novel stem cells marker and a valuable predictor of poor survival NPC.
- Published
- 2013
27. Reduced PDCD4 Expression Promotes Cell Growth Through PI3K/Akt Signaling in Non-Small Cell Lung Cancer
- Author
-
Bin Wu, Dong Wu, Jing Huang, Yan Zhen, Dongming Li, Weimin Yao, Wen Li, Weiyi Fang, Yujie Huang, Aibing Wu, Quanchao Lyu, Yajun Wang, Hongli Gu, Jun Wu, and Min Chen
- Subjects
0301 basic medicine ,Cancer Research ,Cell cycle checkpoint ,Lung Neoplasms ,Gene Expression ,Non-small cell lung cancer (NSCLC) ,Article ,03 medical and health sciences ,Phosphatidylinositol 3-Kinases ,Cell growth ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Humans ,Cyclin D1 ,RNA, Small Interfering ,Autocrine signalling ,Protein kinase B ,neoplasms ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,PI3K/Akt ,PDCD4 ,Chemistry ,Cyclin-Dependent Kinase 4 ,RNA-Binding Proteins ,General Medicine ,Cell Cycle Checkpoints ,Cell cycle ,respiratory tract diseases ,030104 developmental biology ,Oncology ,Tumor progression ,030220 oncology & carcinogenesis ,Gene Knockdown Techniques ,Cancer research ,RNA Interference ,Signal transduction ,Apoptosis Regulatory Proteins ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
It is largely recognized that PDCD4 is frequently lost in tumors of various origins, including lung cancer, and its loss contributes to tumor progression. However, its role and molecular mechanism remain largely unexplored in non-small cell lung cancer (NSCLC). In this study, downregulated PDCD4 mRNA expression was found in NSCLC tissues compared to their corresponding paracarcinoma tissues and distal paracarcinoma tissues. Induced expression of PDCD4 inhibited cell growth and proliferation and cell cycle transition in vitro. Conversely, knocking down PDCD4 expression promoted cell growth and proliferation. Mechanistically, PDCD4 inactivated PI3K/Akt signaling and its downstream cell cycle factors CCND1 and CDK4 to regulate cell growth in NSCLC. Additionally, PI3K-specific inhibitor Ly294002 suppressed the expression of pPI3K (Tyr458), pAkt (Ser473), CCND1, and CDK4 in PC9-shPDCD4 and A549-shPDCD4 cells. Furthermore, Akt-specific inhibitor MK2206 inhibited the expression of pAkt (Ser473), CCND1, and CDK4 in PC9-shPDCD4 and A549-shPDCD4 cells. Taken together, our study provides evidence that PDCD4 inhibits cell growth through PI3K/Akt signaling in NSCLC and may be a potential therapeutic target for NSCLC.
- Published
- 2016
28. Upregulation of microRNA-492 induced by epigenetic drug treatment inhibits the malignant phenotype of clear cell renal cell carcinoma in vitro
- Author
-
Jinmei Li, Kunpeng Wu, Mingchun Li, Lingli Bao, Aibing Wu, Zhixiong Yang, Xiang Shen, and Shunjun Li
- Subjects
Epigenomics ,Cancer Research ,Bisulfite sequencing ,Apoptosis ,Biology ,medicine.disease_cause ,Biochemistry ,Downregulation and upregulation ,Cell Line, Tumor ,microRNA ,Biomarkers, Tumor ,Genetics ,medicine ,Humans ,Neoplasm Invasiveness ,Carcinoma, Renal Cell ,Molecular Biology ,Cell Proliferation ,Cell growth ,DNA Methylation ,Cell cycle ,medicine.disease ,Phenylbutyrates ,Gene Expression Regulation, Neoplastic ,Histone Deacetylase Inhibitors ,MicroRNAs ,Clear cell renal cell carcinoma ,Oncology ,DNA methylation ,Cancer research ,Molecular Medicine ,CpG Islands ,Carcinogenesis - Abstract
Clear cell renal cell carcinoma (ccRCC) is the most common type of cancer of the renal parenchyma. MicroRNAs (miRNAs) are non-coding RNAs of ~22 nucleotides in length, which function as post‑transcriptional regulators. Recently, the downregulation of miRNA (miR)-492 was observed to be associated with ccRCC; however, the molecular mechanism by which miR492 inhibited ccRCC remained to be elucidated. In the present study, it was demonstrated that miR-492 was markedly downregulated in ccRCC tissues when compared with adjacent normal tissues, as determined by reverse transcription-quantitative poymerase chain reaction (PCR). This downregulation was predominantly due to the hypermethylation of the CpG island of the miR-492 promoter, which was detected by methylation specific PCR and bisulfite genomic sequencing PCR, and was shown to inhibit miR-492 transcription. Through the use of a DNA demethylation agent, 5-aza-2'-deoxycytidine or the histone deacetylase inhibitor 4-phenylbutyric acid, the expression level of miR-492 was significantly upregulated in ccRCC cells, which further inhibited cell proliferation and invasion, while promoting cell apoptosis and adhesion. In conclusion, the present study provided novel insights into the potential mechanisms involved in ccRCC and it is hypothesized that miR-492 may become a promising therapeutic agent in the treatment of ccRCC.
- Published
- 2012
29. LATS2 as a poor prognostic marker regulates non-small cell lung cancer invasion by modulating MMPs expression
- Author
-
Haiyin Ye, Kangwen Guo, Hualin Chen, Yanli Mo, Yuzhou Wang, Aibing Wu, Zongjiong Mai, Jinmei Li, Shujun Li, Kunpeng Wu, Yiping Luo, Zhixiong Yang, and Weiren Luo
- Subjects
0301 basic medicine ,Oncology ,Male ,medicine.medical_specialty ,Lung Neoplasms ,Cell ,Kaplan-Meier Estimate ,Biology ,Matrix metalloproteinase ,Protein Serine-Threonine Kinases ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,medicine ,Biomarkers, Tumor ,Humans ,Neoplasm Invasiveness ,RNA, Small Interfering ,Lung cancer ,Lung ,Proportional Hazards Models ,Pharmacology ,Tumor Suppressor Proteins ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,respiratory tract diseases ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,Cell culture ,030220 oncology & carcinogenesis ,Multivariate Analysis ,Immunohistochemistry ,Matrix Metalloproteinase 2 ,Female ,Carcinogenesis - Abstract
Large tumor suppressor 2 (LATS2) plays significant roles in tumorigenesis and cancer progression. This study was aimed to analyze the correlation between LATS2 expression and clinicopathologic features and its prognostic significance in non-small cell lung cancer (NSCLC). LATS2 expression was examined in 73 NSCLC clinical specimens and 22 normal lung tissues using immunohistochemistry. Low levels of LATS2 protein were inversely associated with the T classification (P=0.001), N classification (P=0.005) and clinical stage (P=0.001) in NSCLC patients. Patients with lower LATS2 expression had a significantly shorter overall survival than patients with high LATS2 expression. Multivariate analysis suggested that low expression of LATS2 was an independent prognostic indicator (P=0.002) for the survival of patients with NSCLC. Furthermore, overexpression of LATS2 resulted in mobility inhibition in NSCLC cell lines A549 and H1299, and reduced protein level of matrix metalloproteinase-2 (MMP-2) and MMP-9. On the contrary, LATS2 siRNA treatment enhanced cell mobility and increased MMP-2 and MMP-9 protein expression level. In conclusion, low expression of LATS2 is a potential unfavorable prognostic factor and promoted cell invasion and migration in NSCLC.
- Published
- 2015
30. Magnetic properties of nanocrystalline Fe/Fe3C composites
- Author
-
Hua Yang, Lianxiang Yu, Aibing Wu, Deming Liu, and Lizhu Tong
- Subjects
Nanostructure ,Materials science ,Ferromagnetic material properties ,Composite number ,General Chemistry ,Condensed Matter Physics ,Nanocrystalline material ,chemistry.chemical_compound ,Magnetization ,chemistry ,Transmission electron microscopy ,Particle ,General Materials Science ,Composite material ,Melamine - Abstract
Nanocrystalline Fe/Fe3C composites have been fabricated by an efficient solid-state synthesis route. In this reaction process, we choose the organic compound melamine as the carbon source and hydrothermally prepared nanocrystalline Fe/Fe3O4 composite as metal precursor. X-ray diffraction, transmission electron microscopy, high-resolution transmission electron microscopy and vibrating sample magnetometry have been used to characterize the composites. The experimental results show that changing reaction temperature, reaction time and the amount of melamine allows control over the composition of Fe/Fe3C composites, particle morphology and magnetic properties. The possible formation mechanisms of as-prepared nanostructures with different morphologies are discussed briefly. Magnetization measurements indicate that the composites display ferromagnetic properties at room temperature.
- Published
- 2011
31. Magnetic properties of FexCo1−x/CoyFe1−yFe2O4 composite under hydrothermal condition
- Author
-
Hua Yang, Qin Wang, Aibing Wu, and Shuiming Li
- Subjects
Materials science ,Cobalt ferrite ,Metallurgy ,Spinel ,Alloy ,Composite number ,Analytical chemistry ,engineering ,General Physics and Astronomy ,General Materials Science ,engineering.material ,Redox ,Hydrothermal circulation - Abstract
The metal–ferrite composites Fe x Co 1− x /Co y Fe 1− y Fe 2 O 4 are synthesized by using disproportion of Fe (II) and reduction of Co (II) by Fe 0 under hydrothermal condition. The size of the particles of the composites decreases as the [KOH] decreasing. The composites are measured by TEM and it can be deduced that when [KOH] = 0.1, the size of the alloy body-centered cubic (BCC) in composites is 20 ± 7 nm, the size of the Cobalt ferrite (spinel) is 170 ± 50 nm. The maximal value of the saturation magnetization (Ms) of the composite is about 100.14 emu/g, which is synthesized under Co (II)/Fe (II) = 0.05, [KOH] = 1 N, T = 150 °C and t = 3 h. The value of Hc of the composite synthesized under Co (II)/Fe (II) = 0.5, t = 3 h, T = 150 °C and [KOH] = 10.2 mol/L is about 2878.19 Oe. The Fe–Co alloy is synthesized through a reduction reaction of the composites in a flowing gaseous mixture. There is a maximal value (302.9 emu/g) of the Ms for the alloys generated at 1000 °C, which is the Co 0.412 Fe 0.588 alloy.
- Published
- 2009
32. Nanocomposites of Iron−Cobalt Alloy and Magnetite: Controllable Solvothermal Synthesis and Their Magnetic Properties
- Author
-
Hua Yang, Qin Wang, Lianxiang Yu, Wei Xu, Aibing Wu, and Zhilong Liu
- Subjects
Nanocomposite ,Materials science ,Alloy ,Solvothermal synthesis ,Nanotechnology ,Coercivity ,engineering.material ,equipment and supplies ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,General Energy ,Ferromagnetism ,Chemical engineering ,chemistry ,Transmission electron microscopy ,engineering ,Magnetic nanoparticles ,Physical and Theoretical Chemistry ,human activities ,Magnetite - Abstract
Nanocomposites of Fe−Co alloy and cobalt-substituted magnetite magnetic materials FexCo1−x/CoyRezFe3−y−zO4 (Re = none, La, Nd, and Gd) have been obtained successfully employing a surfactant-assisted solvothermal method. The nanocomposite features hard and soft magnetic phases juxtaposed within one discrete, anisotropic structure. Sodium oleate molecules were found effective in preventing the oxidation of the Fe−Co alloy and aggregation of the magnetic microspheres. X-ray diffraction, X-ray photoelectron spectroscopy, field emission scanning electron microscopy, and transmission electron microscopy were used to characterize the structure and the morphology of the products. The result of magnetic characterization reveals that the magnetic microspheres exhibit a ferromagnetic behavior and possess high saturation magnetization and low coercivity. It is expected that these magnetic microspheres with uniform size would have potential applications such as magnetic carriers for drug targeting because of their exc...
- Published
- 2009
33. The synthesis and the magnetic properties of Gd3+-doped FexCo1−x/CoyFe3−yO4 micro-octahedrons composites
- Author
-
Shuiming Li, Aibing Wu, Qin Wang, and Hua Yang
- Subjects
Materials science ,Scanning electron microscope ,Alloy ,Spinel ,Doping ,Oxide ,Coercivity ,engineering.material ,Condensed Matter Physics ,Hydrothermal circulation ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,chemistry ,Phase (matter) ,engineering ,Composite material - Abstract
Gd 3+ -substituted micro-octahedron composites (Fe x Co 1− x /Co y Gd z Fe 3− y − z O 4 ) in which the Fe–Co alloy has either a bcc or fcc structure and the oxide is a spinel phase were fabricated by the hydrothermal method. The X-ray diffraction (XRD) patterns indicate that the as-synthesized Gd 3+ -substituted micro-octahedron composites are well crystallized. Scanning electron microscopy (SEM) images show that the final product consists of larger numbers of micro-octahedrons with the size ranging from 1.3 to 5 μm, and the size of products are increased with increasing the concentration of KOH. The effect of the Co 2+ /Fe 2+ ratio (0⩽Co 2+ /Fe 2+ ⩽1) and substitution Fe 3+ ions by Gd 3+ ions on structure, magnetic properties of the micro-octahedrons composites were investigated, and a possible growth mechanism is suggested to explain the formation of micro-octahedrons composites. The magnetic properties of the structure show the maximal saturation magnetization (107 emu/g) and the maximal coercivity (1192 Oe) detected by a vibrating sample magnetometer.
- Published
- 2009
34. Let-7a inhibits migration, invasion and epithelial-mesenchymal transition by targeting HMGA2 in nasopharyngeal carcinoma
- Author
-
Jinmei Li, Yanming Lin, Shujun Li, Lixia Li, Yuzhou Wang, Zhixiong Yang, Kunpeng Wu, Xiang Shen, Yanli Mo, and Aibing Wu
- Subjects
Male ,HMGA2 ,Epithelial-Mesenchymal Transition ,Molecular Sequence Data ,Nasopharyngeal neoplasm ,Down-Regulation ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,Cell Movement ,Cell Line, Tumor ,microRNA ,otorhinolaryngologic diseases ,medicine ,Nasopharyngeal carcinoma ,Humans ,Neoplasm Invasiveness ,Epithelial–mesenchymal transition ,3' Untranslated Regions ,Aged ,Cell Proliferation ,Feedback, Physiological ,biology ,Base Sequence ,Cell growth ,Research ,Carcinoma ,HMGA2 Protein ,Cell migration ,MicroRNA ,Let-7a ,Nasopharyngeal Neoplasms ,General Medicine ,Transfection ,Middle Aged ,medicine.disease ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Gene Knockdown Techniques ,biology.protein ,Cancer research ,Female ,Protein Binding - Abstract
Background Let-7a has been shown to play important roles in nasopharyngeal carcinoma (NPC) cell proliferation and apoptosis, but little is known about the function and mechanism of let-7a in nasopharyngeal carcinoma metastasis. We aimed to investigate the function and mechanism of let-7a in nasopharyngeal carcinoma metastasis and clarified the regulation of high mobility group A2 (HMGA2) by let-7a. Methods The expression levels of let-7a and HMGA2 were examined in NPC clinical specimens using quantitative reverse transcription-PCR (RT-qPCR). HMGA2 was confirmed as a target of let-7a through luciferase reporter assays, RT-qPCR, and Western blotting. Furthermore, the roles of let-7a and HMGA2 in regulating NPC cells biological properties including proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process were analyzed with let-7a mimics and si-HMGA2 transfected cells. Results Our study demonstrated that let-7a was downregulated and inversely associated with the clinical stage, T classification and N classification, and HMGA2 was upregulated and directly associated with the clinical stage and N classification in patients with NPC. Moreover, there was an inverse correlation between let-7a expression and HMGA2 expression in NPC patient. In addition, HMGA2 was negatively regulated at the posttranscriptional level by let-7a via a binding site of HMGA2-3′UTR. In addition, synthetic let-7a mimics suppressed NPC cells migration, invasion and EMT process and knockdown of HMGA2 was consistent with the effects of let-7a in NPC cells. Conclusion Let-7a directly downregulates HMGA2 protein expression, which suppress NPC cell migration, invasion and EMT process. Let-7a could serve as a potential diagnostic marker and therapeutic target for NPC. Electronic supplementary material The online version of this article (doi:10.1186/s12967-015-0462-8) contains supplementary material, which is available to authorized users.
- Published
- 2015
35. Concurrent versus sequential whole brain radiotherapy and TKI in EGFR-mutated NSCLC patients with brain metastasis: A single institution retrospective analysis.
- Author
-
Hualin Chen, Aibing Wu, Hua Tao, Donghong Yang, Yiping Luo, Shujun Li, Zhixiong Yang, Ming Chen, Chen, Hualin, Wu, Aibing, Tao, Hua, Yang, Donghong, Luo, Yiping, Li, Shujun, Yang, Zhixiong, and Chen, Ming
- Published
- 2018
- Full Text
- View/download PDF
36. [Clinical significance of MMP2 overexpression in endometrial adenocarcinoma]
- Author
-
Shujun, Li, Xiang, Shen, Zhixiong, Yang, Aibing, Wu, Zhi, Tang, Mingchun, Li, and Yingxia, Ling
- Subjects
Adult ,Aged, 80 and over ,Biomarkers, Tumor ,Humans ,Matrix Metalloproteinase 2 ,Female ,Middle Aged ,Aged ,Endometrial Neoplasms - Abstract
To explore the expression of MMP2 and its correlation with the clinical features and prognosis of endometrial adenocarcinoma.We collected paraffin-embedded samples from 81 patients with endometrial adenocarcinoma aged 32 to 80 years to examine the expression of MMP2 using immunohistochemistry. The correlation of MMP2 expression with the clinical characteristics and prognosis of the patients was analyzed.MMP2 protein was expressed in the cytoplasm of the tumor cells. MMP2 over-expression was negatively correlated with tumor differentiation (P=0.015) and prognosis (P=0.041) of endometrial adenocarcinoma.MMP2 over-expression is a potential malignant biomarker in endometrial adenocarcinoma.
- Published
- 2014
37. Knocking down CDK4 mediates the elevation of let-7c suppressing cell growth in nasopharyngeal carcinoma
- Author
-
Chen Yan, Yajie Zhang, Cheng Chao, Weiyi Fang, Shengni Hua, Qiangyun Wu, Zhen Liu, Aibing Wu, and Xiaobin Long
- Subjects
Cancer Research ,endocrine system ,CDK4 ,Nasopharyngeal neoplasm ,Cyclin-dependent kinase ,Cell Line, Tumor ,Genetics ,Medicine ,E2F1 ,Humans ,neoplasms ,Cell Proliferation ,let-7c ,Nasopharyngeal Carcinoma ,biology ,business.industry ,Cell growth ,Cyclin-dependent kinase 4 ,Carcinoma ,Cell Cycle ,Cyclin-Dependent Kinase 4 ,Nasopharyngeal Neoplasms ,Cell cycle ,medicine.disease ,Prognosis ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Nasopharyngeal carcinoma ,Oncology ,Gene Knockdown Techniques ,Cancer research ,biology.protein ,Cyclin-dependent kinase 6 ,biological phenomena, cell phenomena, and immunity ,business ,NPC ,Signal Transduction ,Research Article - Abstract
Background CDK4 is a protein kinase in the CDK family important for G1/S phase cell cycle progression. However, the roles and molecular mechanisms of CDK4 triggering nasopharynx carcinogenesis are still unclear. Methods Lentiviral-vector mediated shRNA was used to suppress CDK4 expression and examine its molecular mechanisms. Using immunohistochemistry, we analyzed CDK4 protein expression in clinicopathologically characterized nasopharyngeal carcinoma (NPC) cases and nasopharyngeal tissues (NPs). Survival curves were plotted by the Kaplan-Meier method and compared using the log-rank test. Results In this investigation, we knocked down CDK4 expression and observed that NPC cell growth and cell cycle progression were significantly blocked by suppressing expression of CCND1, CDK6, and E2F1 as well as elevated p21 expression. Further, we found that reduced CDK4 expression elevated the expression of let-7c, a tumor-suppressive miRNA modulated by E2F1. We found that let-7c was markedly downregulated in NPC tissues compared to NPs and suppressed cell growth and cell cycle progression by modulating p15/p16/CDK4/E2F1 pathway. Finally, CDK4 protein was observed to be overexpressed in NPC tissues and could be considered an unfavorable prognosis factor for NPC patients although its independent prognostic value did not reach statistical significance (p = 0.087). Conclusions Our results demonstrated that overexpressed CDK4 is an unfavorable prognostic factor which suppresses the expression of tumor suppressive-factor let-7c through p21/CCND1/CDK6/E2F1 signaling, and inhibits cell proliferation by p15/p16/CDK4/E2F1 feedback signaling in NPC.
- Published
- 2013
38. Overexpressed DNA-binding protein inhibitor 2 as an unfavorable prognosis factor promotes cell proliferation in nasopharyngeal carcinoma
- Author
-
Weiren Luo, Huiling Yang, Xin Li, Hao Wang, Weiyi Fang, Aibing Wu, Jing Chen, Kaitai Yao, Xiaoli Yu, Zhen Liu, and Yan Zhen
- Subjects
Adult ,Male ,Biophysics ,Kaplan-Meier Estimate ,Biology ,Biochemistry ,Small hairpin RNA ,RNA interference ,Cell Line, Tumor ,Nasopharynx ,otorhinolaryngologic diseases ,medicine ,Biomarkers, Tumor ,Humans ,RNA, Messenger ,Aged ,Cell Proliferation ,Inhibitor of Differentiation Protein 2 ,Nasopharyngeal Carcinoma ,Cell growth ,Carcinoma ,RNA ,Cell migration ,Nasopharyngeal Neoplasms ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,stomatognathic diseases ,Cell nucleus ,medicine.anatomical_structure ,Nasopharyngeal carcinoma ,Cell culture ,Lymphatic Metastasis ,Cancer research ,Female ,Snail Family Transcription Factors ,Transcription Factors - Abstract
The aim of the present study was to analyze the expression of DNA-binding protein inhibitor 2 (ID2) in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathological features. It was found that the expression of ID2 was significantly increased in NPC cells when compared with that in NP69 cell line. Similar level of ID2 cytoplasmic expression was observed in NPC when compared with that in non-cancerous nasopharynx tissues. However, the level of ID2 in nucleus was increased in NPC when compared with that in normal nasopharynx tissues. Furthermore, the higher expression level of nuclear ID2 was significantly associated with tumor size (T classification), lymph node metastasis (N classification), and clinical stage. Patients with increased ID2 expression level had poorer overall survival rates than those with low ID2 levels. The inhibition of ID2 expression in NPC cell line SUNE1 by lentiviral-mediated short hairpin RNA could suppress cell proliferation and colony formation, but did not disrupt cell migration. Knocking down the expression of ID2 by RNA interference could down-regulate the expression of Snail, suggesting that ID2-promoted cell growth, partially attributing to the regulation of Snail activity in NPC. Our study demonstrated that over-expression of ID2 protein is an unfavorable prognostic factor which promotes cell proliferation in NPC.
- Published
- 2012
39. ZEB2 mediates multiple pathways regulating cell proliferation, migration, invasion, and apoptosis in glioma
- Author
-
Zhen Liu, Hao Long, Xiaoli Yu, Yan Zhen, Yuping Peng, Aibing Wu, Hao Wang, Ying Zhou, Songtao Qi, Ye Song, Lu-xiong Fang, Weiren Luo, Zhiyong Li, Chunping Mai, Weiyi Fang, and Yan Chen
- Subjects
Small interfering RNA ,Multidisciplinary ,Cyclin E ,Cell growth ,Science ,Cell migration ,Cell cycle ,Biology ,medicine.disease ,Cyclin D1 ,Glioma ,medicine ,Cancer research ,E2F1 ,Medicine - Abstract
BackgroundThe aim of the present study was to analyze the expression of Zinc finger E-box Binding homeobox 2 (ZEB2) in glioma and to explore the molecular mechanisms of ZEB2 that regulate cell proliferation, migration, invasion, and apoptosis.Methodology/principal findingsExpression of ZEB2 in 90 clinicopathologically characterized glioma patients was analyzed by immunohistochemistry. Furthermore, siRNA targeting ZEB2 was transfected into U251 and U87 glioma cell lines in vitro and proliferation, migration, invasion, and apoptosis were examined separately by MTT assay, Transwell chamber assay, flow cytometry, and western blot.ResultsThe expression level of ZEB2 protein was significantly increased in glioma tissues compared to normal brain tissues (PConclusionOverexpression of ZEB2 is an unfavorable factor that may facilitate glioma progression. Knockdown ZEB2 expression by siRNA suppressed cell proliferation, migration, invasion and promoted cell apoptosis in glioma cells.
- Published
- 2012
40. Elevated expression of CDK4 in lung cancer
- Author
-
Jinsong Guo, Zhixiong Yang, Zhen Liu, Xiaoli Yu, Weiyi Fang, Yan Zhen, Weiren Luo, Aibing Wu, Dong Wu, Hao Wang, Bin Wu, Huiling Yang, and Ying Zhou
- Subjects
Cyclin-Dependent Kinase Inhibitor p21 ,Male ,Pathology ,medicine.medical_specialty ,Lung Neoplasms ,lcsh:Medicine ,Down-Regulation ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Small hairpin RNA ,Cell Movement ,Cell Line, Tumor ,medicine ,Humans ,Lung cancer ,Lung ,Cell Proliferation ,Proportional Hazards Models ,Medicine(all) ,Regulation of gene expression ,integumentary system ,Biochemistry, Genetics and Molecular Biology(all) ,Kinase ,Cell growth ,Cyclin-dependent kinase 4 ,Research ,lcsh:R ,Cell Cycle ,Cyclin-Dependent Kinase 4 ,General Medicine ,Cell cycle ,medicine.disease ,Immunohistochemistry ,Survival Analysis ,Gene Expression Regulation, Neoplastic ,Gene Knockdown Techniques ,Multivariate Analysis ,Cancer research ,biology.protein ,Female - Abstract
Background The aim of the present study was to analyze the expression of Cyclin-dependent kinase 4 (CDK4) in lung cancer and its correlation with clinicopathologic features. Furthermore, the involvement of CDK4-mediated cell cycle progression and its molecular basis were investigated in the pathogenesis of lung cancer. Methods Using immunohistochemistry analysis, we analyzed CDK4 protein expression in 89 clinicopathologically characterized lung cancer patients (59 males and 30 females) with ages ranging from 36 to 78 years and compared them to 23 normal lung tissues. Cases with cytoplasmic and nuclear CDK4 immunostaining score values greater than or equal to 7 were regarded as high expression while scores less than 7 were considered low expression. The correlation between the expression level of CDK4 and clinical features was analyzed. Furthermore, we used lentiviral-mediated shRNA to suppress the expression of CDK4 and investigate its function and molecular mechanism for mediating cell cycle progression. Results The expression level of CDK4 protein was significantly increased in lung cancer tissues compared to normal tissues (P < 0.001). In addition, high levels of CDK4 protein were positively correlated with the status of pathology classification (P = 0.047), lymph node metastasis (P = 0.007), and clinical stage (P = 0.004) of lung cancer patients. Patients with higher CDK4 expression had a markedly shorter overall survival time than patients with low CDK4 expression. Multivariate analysis suggested the level of CDK4 expression was an independent prognostic indicator (P < 0.001) for the survival of patients with lung cancer. Use of lentiviral-mediated shRNA to inhibit the expression of CDK4 in lung cancer cell line A549 not only inhibited cell cycle progression, but also dramatically suppressed cell proliferation, colony formation, and migration. Furthermore, suppressing CDK4 expression also significantly elevated the expression of cell cycle regulator p21 Conclusion Overexpressed CDK4 is a potential unfavorable prognostic factor and mediates cell cycle progression by regulating the expression of p21 in lung cancer
- Published
- 2011
41. Meta-analysis on the association between pathologic complete response and triple-negative breast cancer after neoadjuvant chemotherapy
- Author
-
Aibing Wu, Zhixiong Yang, Qiaozhu Yang, Kunpeng Wu, and Yi Liu
- Subjects
Oncology ,medicine.medical_specialty ,Organoplatinum Compounds ,medicine.medical_treatment ,Breast Neoplasms ,Triple Negative Breast Neoplasms ,pathologic complete response ,breast cancer ,Breast cancer ,Surgical oncology ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Neoplasm Metastasis ,Triple-negative breast cancer ,Neoadjuvant therapy ,Clinical Trials as Topic ,Chemotherapy ,business.industry ,Research ,Remission Induction ,Odds ratio ,Prognosis ,medicine.disease ,Neoadjuvant Therapy ,meta-analysis ,Meta-analysis ,Female ,Surgery ,business ,neoadjuvant chemotherapy - Abstract
Background Triple-negative breast cancer (TNBC) is a special subtype of breast cancer that is characterized by poor prognosis, strong tumor invasion and a high pathologic complete response (pCR) to neoadjuvant chemotherapy (NAC). The pCR rate is a prognostic factor for TNBC. We aimed to evaluate the relationship between pCR and TNBC after NAC and originally tried to identify factors related to achieving pCR for TNBC using a meta-analysis. Methods We systematically searched the literature for pCR and breast cancer after NAC and carefully identified eligibility criteria. The association between pCR and breast cancer subtypes was estimated using Review Manager, while pCR rates for TNBC and non-TNBC were determined using Meta-Analyst. Results This analysis included a total of 9,460 cases from 27 studies. The summary odds ratio estimating the relationship between pCR and breast cancer subtypes (TNBC vs non-TNBC) was 3.02 (95% confidence interval (CI), 2.66 to 3.42). The TNBC pCR rate was 28.9% (95% CI, 27.0 to 30.8%) and the non-TNBC was 12.5% (95% CI, 11.7 to 13.4%). From subgroup analyses, we identified the factors associated with the highest pCR rates for TNBC. Conclusions TNBC has a higher pCR rate than non-TNBC. In the NAC setting, these factors of platinum-containing, more than six cycles, four kinds of drugs, 16 weeks’ treatment duration and sequential chemotherapy may contribute to increasing the pCR rate.
- Published
- 2014
42. Magnetic properties of carbon-encapsulated Fe–Co alloy nanoparticles
- Author
-
Hua Yang, Xuwei Yang, and Aibing Wu
- Subjects
Materials science ,Metallurgy ,Alloy ,technology, industry, and agriculture ,chemistry.chemical_element ,Nanoparticle ,engineering.material ,equipment and supplies ,Carbide ,Inorganic Chemistry ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Transmission electron microscopy ,Impurity ,engineering ,Melamine ,High-resolution transmission electron microscopy ,Carbon - Abstract
Carbon-encapsulated Fe-Co alloy nanoparticles (Fe-Co(C)) have been fabricated with different Co/Fe ratios by an efficient solid-state route using melamine as carbon source. The structure and morphology of Fe-Co(C) nanoparticles were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HRTEM). The XRD characterization results reveal that all products are alloys with no carbide impurity. The TEM and HRTEM observations show that the alloy nanoparticles are encapsulated in carbon shells. Additionally, the reactions involved in the syntheses are postulated. The variation of magnetic properties of Fe-Co(C) with Co/Fe has been discussed according to the room temperature VSM measurement results.
- Published
- 2013
43. Upregulation of microRNA-492 induced by epigenetic drug treatment inhibits the malignant phenotype of clear cell renal cell carcinoma in vitro.
- Author
-
AIBING WU, KUNPENG WU, MINGCHUN LI, LINGLI BAO, XIANG SHEN, SHUNJUN LI, JINMEI LI, and ZHIXIONG YANG
- Subjects
- *
RENAL cell carcinoma , *CANCER , *RENAL hypertension , *MICRORNA , *NUCLEOTIDES , *REVERSE transcriptase polymerase chain reaction - Abstract
Clear cell renal cell carcinoma (ccRCC) is the most common type of cancer of the renal parenchyma. MicroRNAs (miRNAs) are non-coding RNAs of ~22 nucleotides in length, which function as post-transcriptional regulators. Recently, the downregulation of miRNA (miR)-492 was observed to be associated with ccRCC; however, the molecular mechanism by which miR-492 inhibited ccRCC remained to be elucidated. In the present study, it was demonstrated that miR-492 was markedly downregulated in ccRCC tissues when compared with adjacent normal tissues, as determined by reverse transcription-quantitative poymerase chain reaction (PCR). This downregulation was predominantly due to the hypermethylation of the CpG island of the miR-492 promoter, which was detected by methylation specific PCR and bisulfite genomic sequencing PCR, and was shown to inhibit miR-492 transcription. Through the use of a DNA demethylation agent, 5-aza-2'-deoxycytidine or the histone deacetylase inhibitor 4-phenylbutyric acid, the expression level of miR-492 was significantly upregulated in ccRCC cells, which further inhibited cell proliferation and invasion, while promoting cell apoptosis and adhesion. In conclusion, the present study provided novel insights into the potential mechanisms involved in ccRCC and it is hypothesized that miR-492 may become a promising therapeutic agent in the treatment of ccRCC. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
44. Let-7a inhibits migration, invasion and epithelial-mesenchymal transition by targeting HMGA2 in nasopharyngeal carcinoma.
- Author
-
Aibing Wu, Kunpeng Wu, Jinmei Li, Yanli Mo, Yanming Lin, Yuzhou Wang, Xiang Shen, Shujun Li, Lixia Li, and Zhixiong Yang
- Subjects
- *
CELL migration , *DNA-binding proteins , *NASOPHARYNX cancer , *CANCER cell proliferation , *APOPTOSIS , *METASTASIS , *CANCER cell motility - Abstract
Background: Let-7a has been shown to play important roles in nasopharyngeal carcinoma (NPC) cell proliferation and apoptosis, but little is known about the function and mechanism of let-7a in nasopharyngeal carcinoma metastasis. We aimed to investigate the function and mechanism of let-7a in nasopharyngeal carcinoma metastasis and clarified the regulation of high mobility group A2 (HMGA2) by let-7a. Methods: The expression levels of let-7a and HMGA2 were examined in NPC clinical specimens using quantitative reverse transcription-PCR (RT-qPCR). HMGA2 was confirmed as a target of let-7a through luciferase reporter assays, RT-qPCR, and Western blotting. Furthermore, the roles of let-7a and HMGA2 in regulating NPC cells biological properties including proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process were analyzed with let-7a mimics and si-HMGA2 transfected cells. Results: Our study demonstrated that let-7a was downregulated and inversely associated with the clinical stage, T classification and N classification, and HMGA2 was upregulated and directly associated with the clinical stage and N classification in patients with NPC. Moreover, there was an inverse correlation between let-7a expression and HMGA2 expression in NPC patient. In addition, HMGA2 was negatively regulated at the posttranscriptional level by let-7a via a binding site of HMGA2-3'UTR. In addition, synthetic let-7a mimics suppressed NPC cells migration, invasion and EMT process and knockdown of HMGA2 was consistent with the effects of let-7a in NPC cells. Conclusion: Let-7a directly downregulates HMGA2 protein expression, which suppress NPC cell migration, invasion and EMT process. Let-7a could serve as a potential diagnostic marker and therapeutic target for NPC. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
45. Knocking down CDK4 mediates the elevation of let-7c suppressing cell growth in nasopharyngeal carcinoma.
- Author
-
Zhen Liu, Xiaobin Long, Cheng Chao, Chen Yan, Qiangyun Wu, Shengni Hua, Yajie Zhang, Aibing Wu, and Weiyi Fang
- Subjects
NASOPHARYNX cancer ,CYCLIN-dependent kinases ,GROWTH factors ,GENE expression ,MICRORNA ,IMMUNOHISTOCHEMISTRY - Abstract
Background CDK4 is a protein kinase in the CDK family important for G1/S phase cell cycle progression. However, the roles and molecular mechanisms of CDK4 triggering nasopharynx carcinogenesis are still unclear. Methods Lentiviral-vector mediated shRNA was used to suppress CDK4 expression and examine its molecular mechanisms. Using immunohistochemistry, we analyzed CDK4 protein expression in clinicopathologically characterized nasopharyngeal carcinoma (NPC) cases and nasopharyngeal tissues (NPs). Survival curves were plotted by the Kaplan-Meier method and compared using the log-rank test. Results In this investigation, we knocked down CDK4 expression and observed that NPC cell growth and cell cycle progression were significantly blocked by suppressing expression of CCND1, CDK6, and E2F1 as well as elevated p21 expression. Further, we found that reduced CDK4 expression elevated the expression of let-7c, a tumor-suppressive miRNA modulated by E2F1. We found that let-7c was markedly downregulated in NPC tissues compared to NPs and suppressed cell growth and cell cycle progression by modulating p15/p16/CDK4/E2F1 pathway. Finally, CDK4 protein was observed to be overexpressed in NPC tissues and could be considered an unfavorable prognosis factor for NPC patients although its independent prognostic value did not reach statistical significance (p = 0.087). Conclusions Our results demonstrated that overexpressed CDK4 is an unfavorable prognostic factor which suppresses the expression of tumor suppressive-factor let-7c through p21/CCND1/CDK6/E2F1 signaling, and inhibits cell proliferation by p15/p16/CDK4/E2F1 feedback signaling in NPC. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
46. Meta-analysis on the association between pathologic complete response and triple-negative breast cancer after neoadjuvant chemotherapy.
- Author
-
Kunpeng Wu, Qiaozhu Yang, Yi Liu, Aibing Wu, and Zhixiong Yang
- Subjects
META-analysis ,BREAST cancer diagnosis ,BREAST cancer prognosis ,CANCER chemotherapy ,CONFIDENCE intervals - Abstract
Background Triple-negative breast cancer (TNBC) is a special subtype of breast cancer that is characterized by poor prognosis, strong tumor invasion and a high pathologic complete response (pCR) to neoadjuvant chemotherapy (NAC). The pCR rate is a prognostic factor for TNBC. We aimed to evaluate the relationship between pCR and TNBC after NAC and originally tried to identify factors related to achieving pCR for TNBC using a meta-analysis. Methods We systematically searched the literature for pCR and breast cancer after NAC and carefully identified eligibility criteria. The association between pCR and breast cancer subtypes was estimated using Review Manager, while pCR rates for TNBC and non-TNBC were determined using Meta-Analyst. Results This analysis included a total of 9,460 cases from 27 studies. The summary odds ratio estimating the relationship between pCR and breast cancer subtypes (TNBC vs non-TNBC) was 3.02 (95% confidence interval (CI), 2.66 to 3.42). The TNBC pCR rate was 28.9% (95% CI, 27.0 to 30.8%) and the non-TNBC was 12.5% (95% CI, 11.7 to 13.4%). From subgroup analyses, we identified the factors associated with the highest pCR rates for TNBC. Conclusions TNBC has a higher pCR rate than non-TNBC. In the NAC setting, these factors of platinumcontaining, more than six cycles, four kinds of drugs, 16 weeks' treatment duration and sequential chemotherapy may contribute to increasing the pCR rate. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
47. Elevated expression of CDK4 in lung cancer.
- Author
-
Aibing Wu, Bin Wu, Jinsong Guo, Weiren Luo, Dong Wu, Huiling Yang, Yan Zhen, Xiaoli Yu, Hao Wang, Ying Zhou, Zhen Liu, Weiyi Fang, Zhixiong Yang, Wu, Aibing, Wu, Bin, Guo, Jinsong, Luo, Weiren, Wu, Dong, Yang, Huiling, and Zhen, Yan
- Subjects
- *
CANCER patients , *CYCLIN-dependent kinases , *LUNG cancer , *IMMUNOHISTOCHEMISTRY , *CELL lines - Abstract
Background: The aim of the present study was to analyze the expression of Cyclin-dependent kinase 4 (CDK4) in lung cancer and its correlation with clinicopathologic features. Furthermore, the involvement of CDK4-mediated cell cycle progression and its molecular basis were investigated in the pathogenesis of lung cancer.Methods: Using immunohistochemistry analysis, we analyzed CDK4 protein expression in 89 clinicopathologically characterized lung cancer patients (59 males and 30 females) with ages ranging from 36 to 78 years and compared them to 23 normal lung tissues. Cases with cytoplasmic and nuclear CDK4 immunostaining score values greater than or equal to 7 were regarded as high expression while scores less than 7 were considered low expression. The correlation between the expression level of CDK4 and clinical features was analyzed. Furthermore, we used lentiviral-mediated shRNA to suppress the expression of CDK4 and investigate its function and molecular mechanism for mediating cell cycle progression.Results: The expression level of CDK4 protein was significantly increased in lung cancer tissues compared to normal tissues (P < 0.001). In addition, high levels of CDK4 protein were positively correlated with the status of pathology classification (P = 0.047), lymph node metastasis (P = 0.007), and clinical stage (P = 0.004) of lung cancer patients. Patients with higher CDK4 expression had a markedly shorter overall survival time than patients with low CDK4 expression. Multivariate analysis suggested the level of CDK4 expression was an independent prognostic indicator (P < 0.001) for the survival of patients with lung cancer. Use of lentiviral-mediated shRNA to inhibit the expression of CDK4 in lung cancer cell line A549 not only inhibited cell cycle progression, but also dramatically suppressed cell proliferation, colony formation, and migration. Furthermore, suppressing CDK4 expression also significantly elevated the expression of cell cycle regulator p21Conclusion: Overexpressed CDK4 is a potential unfavorable prognostic factor and mediates cell cycle progression by regulating the expression of p21 in lung cancer. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
48. Investigation of distribution uniformity of distributor for biogas slurry application based on CFD analysis.
- Author
-
Jingjing Fu, Yongsheng Chen, Binxing Xu, Biao Ma, Pengjun Wang, Aibing Wu, and Mingjiang Chen
- Subjects
- *
BIOGAS , *SLURRY , *FLOW coefficient , *NON-Newtonian fluids , *RHEOLOGY , *UNIFORMITY , *FLOW velocity - Abstract
A horizontal distributor for biogas slurry application was proposed to explore the distribution performance through CFD analysis and verified by field test. The rheological properties of biogas slurry were analyzed at first, and key parameters were obtained for the next simulation. The effects of distribution modes, inlet direction, and outlets number on the velocity distribution of flow field and mass flow rate of the horizonal distributor were investigated by CFD simulations. Results of rheological properties indicated that biogas slurry was a non-Newtonian fluid and exhibited shear-thinning behavior. It can be well described by power-law model. The simulation results showed that the geometry of rotor, especially the block numbers was the main factor that determining the fluid movement and trajectory of distribution and output. The mode rotor 1 with two blocks reached the lowest variable coefficient of mass flow rate (4.49%), indicating a higher degree of uniformity. The upward inlet direction would obtain less dead zone, and the distributor with an even outlets number would possess more uniform distribution and less dead zone. The field test of the distributor with rotor 1, upward inlet direction, and 24 outlets has been carried on to verify the simulation results, the variable coefficient of mass flow was 13.06%, which was slightly higher than the simulation (9.23%), but it still within the range of requirement (<15%). The proposed model and the findings of this work are of guiding significance for the study of the utilization technology and equipment of liquid biogas residue. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.