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Abstract Objectives To develop and validate nomograms combining radiomics and semantic features to identify the invasiveness and histopathological risk stratification of thymic epithelial tumors (TET) using contrast-enhanced CT. Methods This retrospective multi-center study included 224 consecutive cases. For each case, 6764 intratumor and peritumor radiomics features and 31 semantic features were collected. Multi-feature selections and decision tree models were performed on radiomics features and semantic features separately to select the most important features for Masaoka–Koga staging and WHO classification. The selected features were then combined to create nomograms for the two systems. The performance of the radiomics model, semantic model, and combined model was evaluated using the area under the receiver operating characteristic curves (AUCs). Results One hundred eighty-seven cases (56.5 years ± 12.3, 101 men) were included, with 62 cases as the external test set. For Masaoka–Koga staging, the combined model, which incorporated five peritumor radiomics features and four semantic features, showed an AUC of 0.958 (95% CI: 0.912–1.000) in distinguishing between early-stage (stage I/II) and advanced-stage (III/IV) TET in the external test set. For WHO classification, the combined model incorporating five peritumor radiomics features and two semantic features showed an AUC of 0.857 (0.760–0.955) in differentiating low-risk (type A/AB/B1) and high-risk (B2/B3/C) TET. The combined models showed the most effective predictive performance, while the semantic models exhibited comparable performance to the radiomics models in both systems (p > 0.05). Conclusion The nomograms combining peritumor radiomics features and semantic features could help in increasing the accuracy of grading invasiveness and risk stratification of TET. Critical relevance statement Peripheral invasion and histopathological type are major determinants of treatment and prognosis of TET. The integration of peritumoral radiomics features and semantic features into nomograms may enhance the accuracy of grading invasiveness and risk stratification of TET. Key Points Peritumor region of TET may suggest histopathological and invasive risk. Peritumor radiomic and semantic features allow classification by Masaoka–Koga staging (AUC: 0.958). Peritumor radiomic and semantic features enable the classification of histopathological risk (AUC: 0.857). Graphical Abstract

Member 5 of the Upper Triassic Xujiahe Formation (T3X5) in central Sichuan Basin has made a breakthrough in exploration recently. However, this new stratum has not been investigated sufficiently with respect to basic geology, making its types and distribution of sedimentary facies unclear, which severely restricts its subsequent exploration evaluation. In this study, types of sedimentary microfacies in the first sand group of T3X5 (T3X51) are clarified through core observation and logging interpretation using core, log and seismic data, and then distribution of sedimentary microfacies in T3X51 is determined according to seismic waveform features and seismic prediction. The results show that T3X51 in the Dongfengchang area is mainly composed of deltaic deposits of several microfacies, such as delta front underwater distributary channel, sheet sand, and interdistributary bay. On seismic sections, different microfacies are significantly different in waveform features, the underwater distributary channel is characterized by one trough between two peaks, while diversion bay exhibits chaotic reflections between T6 and T51. The sedimentary microfacies varied greatly during the depositional period of T3X51 in the Dongfengchang area, this is because that the sediment supply was mainly controlled by the southwest and southeast provenance regions. Three superimposed underwater distributary channels are developed in the Dongfengchang area. The phase-1 superimposed underwater distributary channel in the northwest transition to sheet sand in the northeast, the phase-2 superimposed underwater distributary channel in the south extends shortly, the phase-3 superimposed underwater distributary channel in the northeast has a large development scale. These research findings are helpful to guide the subsequent exploration of T3X5 gas reservoir and also theoretically significant for investigating the depositional evolution of the Xujiahe Formation in central Sichuan Basin.

Background Hypertension is a significant risk factor for cardiovascular and chronic kidney diseases. Its management in young people remains limited. Device‐based therapies, such as low‐level tragus stimulation (LL‐TS), a noninvasive method that reduces sympathetic activity, have recently been explored for resistant hypertension. Methods and Results This trial involved patients with Grade 1 hypertension with no other medical history. LL‐TS (20 Hz, 1 mA, 1 h/day) was applied for 3 months on the tragus (Intervention group [IG]) or earlobe (Control group [CG]). Blood pressure and outcomes were assessed at the first, second, and third months. Among 40 patients, 21 were in IG and 19 in CG. Baseline systolic blood pressure was similar between IG (142.62±8.18 mm Hg) and CG (143.00±8.61 mm Hg), P=0.89. Post‐LL‐TS, systolic blood pressure showed significant reductions in IG compared with CG at the first (IG: 134.47±5.95 mm Hg, CG: 141.28±6.78 mm Hg, P=0.002), second (IG: 132.50±7.51 mm Hg, CG: 140.62±7.15 mm Hg, P=0.001), and third months (IG: 128.81±7.13 mm Hg, CG: 136.51±7.96 mm Hg, P=0.003). diastolic blood pressure also differed significantly: first month (IG: 85.34±5.81 mm Hg, CG: 89.74±6.32 mm Hg, P=0.03), second month (IG: 82.12±5.22 mm Hg, CG: 88.57±7.11 mm Hg, P=0.002), and third month (IG: 80.71±5.96 mm Hg, CG: 87.55±5.26 mm Hg, P=0.001). Heart rate was unchanged (P>0.05). Only 0.01% of IG subjects reported site irritation, with no serious adverse events. Conclusions LL‐TS led to significant blood pressure reductions in young patients with essential hypertension. Further larger trials are needed to confirm the safety and efficacy of LL‐TS. Registration URL: https://www.chictr.org.cn/; Unique identifier: ChiCTR2000038448.

Palmer Drought Severity Index (PDSI) is a widely used indicator to assess the intensity of drought based on a similar two-layer bucket (TLB) model, especially in arid and semi-arid regions. However, the TLB model cannot quantify surface water balance, effectively, which may lead to the mis-assessment of drought. Therefore, an optimized PDSI indicator named RW-PDSI based on the water balance is constructed. RW-PDSI is developed using the output of a newly proposed remote-sensing-based water balance assessment tool (RWBAT) model. The RWBAT model is constructed to depict the detailed hydrological processes on a fine spatial scale using high-resolution remote sensing data. To test its performance, we collected tree-ring data on the Loess Plateau (LP) to show the drought conditions in past decades (1991–2020). Tree-ring data provide a useful continuous multi-year data archive for drought assessment. Our results showed that the correlation between RW-PDSI and tree-ring data was statistically significant (p

The effects of solid solution temperature, aging temperature and aging cooling rate on the microstructure evolution of forged Ti-22.5Al-24.7Nb (at. %) alloy were investigated. With the increasing solid solution temperature, the number of equiaxed microstructure first increases and then decreases, reaching the maximum at 900 °C. Lamellar microstructure solubilizes in the matrix or gradually becomes coarser and shorter, and transforms into equiaxed microstructure. When the solution temperature is 1000 °C, equiaxed α2 phase gradually changes to be lath-shaped and concave. During the aging process, some α2 phase transforms into O phase, and the aging temperature affects the shape of equiaxed microstructure and the spacing between the needle-like microstructure. With the increasing aging temperature, the number of equiaxed microstructure increases linearly, and the proportion of fine secondary O phase decreases while the thickness increases. The lamellar morphology of O phase can be retained at a faster cooling rate, while a portion of lamellar O phase is converted to the B2 phase after furnace cooling. Through heat treatment, the volume fraction and the size of lamellar O phase could be well controlled.

Primary aldosteronism (PA), often due to aldosteronoma, commonly causes secondary hypertension and typically requires surgery. We present a case of an elderly man with longstanding hypertension, complicated by cerebral hemorrhage and myocardial infarction. Enhanced CT imaging identified a right-sided aldosteronoma and left adrenal gland fullness. Combined with upright supine aldosterone ratio, captopril challenge test, bilateral adrenal venous sampling, and CYP11B1/CYP11B2 fusion gene testing, the diagnosis of PA was confirmed. Despite the absence of surgical intervention in this patient, pharmacotherapy effectively managed hypertension and enhanced cardiac function, thereby underscoring the advantageous utilization of aldosterone antagonists in non-surgical candidates diagnosed with PA.

This study analyzed the petrological characteristics, diagenesis, pore types, and physical properties of the tight coarse-grained siliciclastic sequences in the third member of the Upper Triassic Xujiahe Formation (also referred to as the Xu-3 Member) in the western Yuanba area in the northeastern Sichuan Basin, China, based on the results of 242.61-m-long core description, 292 thin-section observations, scanning electron microscopy (SEM), and 292 physical property tests. The types and genetic mechanisms of high-quality tight coarse-grained siliciclastic reservoirs in this member was determined thereafter. The research objective is to guide the exploration and development of the tight coarse-grained siliciclastic sequences in the Xu-3 Member. The results of this study are as follows. Two types of high-quality reservoirs are developed in the coarse-grained siliciclastic sequences of the Xu-3 Member, namely the fractured fine-grained sandy conglomerate type and porous medium-grained calcarenaceous sandstone type. Hydrodynamic energy in the sedimentary environment is the key factor controlling the formation of high-quality reservoirs. These high-quality reservoirs are developed mainly in the transitional zone with moderately high hydrodynamic energy between delta-plain braided channels and delta-front subaqueous distributary channels. The dolomitic debris (gravel) content is the main factor affecting the reservoirs’ physical properties. The micritic algal debris and sandy debris in the dolomitic debris (or gravels) tend to recrystallize during burial, forming intercrystalline pores within. In the medium-grained calcarenaceous sandstones, intercrystalline pores in the dolomitic debris are formed at the early diagenetic stage, and a pore system consisting of structural fractures connecting intergranular pores, intergranular dissolution pores, and kaolinite intergranular micropores is developed at the late stage of diagenesis. The formation of intercrystalline pores in dolomite gravels and gravel-edge fractures, a pore system connected by gravel-edge and tectonic fractures, is closely related to the dolomite gravels in the sandy fine-grained conglomerates.

Abstract Background Osteoarthritis (OA) is a multifaceted chronic joint disease characterized by complex mechanisms. It has a detrimental impact on the quality of life for individuals in the middle-aged and elderly population while also imposing a significant socioeconomic burden. At present, there remains a lack of comprehensive understanding regarding the pathophysiology of OA. The objective of this study was to examine the genes, functional pathways, and immune infiltration characteristics associated with the development and advancement of OA. Methods The Gene Expression Omnibus (GEO) database was utilized to acquire gene expression profiles. The R software was employed to conduct the screening of differentially expressed genes (DEGs) and perform enrichment analysis on these genes. The OA-characteristic genes were identified using the Weighted Gene Co-expression Network Analysis (WGCNA) and the Lasso algorithm. In addition, the infiltration levels of immune cells in cartilage were assessed using single-sample gene set enrichment analysis (ssGSEA). Subsequently, a correlation analysis was conducted to examine the relationship between immune cells and the OA-characteristic genes. Results A total of 80 DEGs were identified. As determined by functional enrichment, these DEGs were associated with chondrocyte metabolism, apoptosis, and inflammation. Three OA-characteristic genes were identified using WGCNA and the lasso algorithm, and their expression levels were then validated using the verification set. Finally, the analysis of immune cell infiltration revealed that T cells and B cells were primarily associated with OA. In addition, Tspan2, HtrA1 demonstrated a correlation with some of the infiltrating immune cells. Conclusions The findings of an extensive bioinformatics analysis revealed that OA is correlated with a variety of distinct genes, functional pathways, and processes involving immune cell infiltration. The present study has successfully identified characteristic genes and functional pathways that hold potential as biomarkers for guiding drug treatment and facilitating molecular-level research on OA.

In the second member of the Upper Triassic Xujiahe Formation (T3x2) in the Xinchang area, western Sichuan Basin, only a low percent of reserves has been recovered, and the geological model of gas reservoir sweet spot remains unclear. Based on a large number of core, field outcrop, test and logging-seismic data, the T3x2 gas reservoir in the Xinchang area is examined. The concept of fault-fold-fracture body (FFFB) is proposed, and its types are recognized. The main factors controlling fracture development are identified, and the geological models of FFFB are established. FFFB refers to faults, folds and associated fractures reservoirs. According to the characteristics and genesis, FFFBs can be divided into three types: fault-fracture body, fold-fracture body, and fault-fold body. In the hanging wall of the fault, the closer to the fault, the more developed the effective fractures; the greater the fold amplitude and the closer to the fold hinge plane, the more developed the effective fractures. Two types of geological models of FFFB are established: fault-fold fracture, and matrix storage and permeability. The former can be divided into two subtypes: network fracture, and single structural fracture, and the later can be divided into three subtypes: bedding fracture, low permeability pore, and extremely low permeability pore. The process for evaluating favorable FFFB zones was formed to define favorable development targets and support the well deployment for purpose of high production. The study results provide a reference for the exploration and development of deep tight sandstone oil and gas reservoirs in China.

Abstract Background To establish and validate radiomic models combining intratumoral (Intra) and peritumoral (Peri) features obtained from pretreatment MRI for the prediction of treatment response of lymph node metastasis from nasopharyngeal cancer (NPC). Methods One hundred forty-five NPC patients (102 in the training and 43 in the validation set) were retrospectively enrolled. Radiomic features were extracted from Intra and Peri regions on the metastatic cervical lymph node, and selected with the least absolute shrinkage and selection operator (LASSO). Multivariate logistic regression analysis was applied to build radiomic models. Sensitivity, specificity, accuracy, and the area under the curve (AUC) of receiver operating characteristics were employed to evaluate the predictive power of each model. Results The AUCs of the radiomic model of Intra, Peri, Intra + Peri, and Clinical-radiomic were 0.910, 0.887, 0.934, and 0.941, respectively, in the training set and 0.737, 0.794, 0.774, and 0.783, respectively, in the validation set. There were no significant differences in prediction performance among the radiomic models in the training and validation sets (all P > 0.05). The calibration curve of the radiomic model of Peri demonstrated good agreement between prediction and observation in the training and validation sets. Conclusions The pretreatment MRI-based radiomics model may be useful in predicting the treatment response of metastatic lymph nodes of NPC. Besides, the generalization ability of the radiomic model of Peri was better than that of Intra and Intra + Peri.

The Xujiahe Formation in the western Yuanba area of the northeastern Sichuan Basin was slightly affected by late tectonic activity and thus has fewer fractures relative to the central and eastern Yuanba areas. Utilizing core observation, slice identification, logging interpretation, and inclusion analysis, the characteristics and controlling factors of fractures in tight sandstone of the Xujiahe Formation of the western Yuanba area were analyzed.The results show that the study area mainly contains low-angle shear fractures and diagenetic fractures. Fractures in the 2nd and 3rd members of the Xujiahe Formation were primarily filled by calcite and clay respectively. The fractures in the Xujiahe Formation of the study area can be classified into three stages, corresponding to the regional tectonic deformations during the Late Jurassic, the end of the Early Cretaceous, and the Late Himalayan. The fractures of the 1st and 2nd stages were fully or partly filled, but the 3rd stage fracture was not filled. The development of fractures was mainly affected by the fault and fold deformation strength, lithology combination, and stratum thickness. Fractures in the hinge zone and southeast limb of Jiulongshan anticline were mainly generated by bending deformation except for the fault zone. The fault-related fracture was more plentiful in the vicinity of the fault zone. Both bending deformation fracture and fault-related fracture are favorable for petroleum development. Fractures in the conglomerate of 3rd Member of the Xujiahe Formation can terminate at or transect the gravel particle and generally show small apertures. The low-angle fractures and micro-fractures in the quartz sandstones of the 2nd Member of the Xujiahe Formation developed aligning with bedding. Both the 3rd and 2nd members of the Xujiahe Formation in the study area are favorable targets for petroleum exploration and development.

Identifying the potential pollution sources of surface water pollutants is essential for the management and protection of regional water environments in drinking water source areas. In this study, absolute principal component score-multiple linear regression (APCS-MLR) and positive matrix factorization (PMF) models were applied to assess water quality and identify the potential pollution sources affecting the surface water quality of Xin’an River Basin. For this purpose, a 10-year (2011–2020) dataset of eight water quality indicators (including pH, EC, DO, COD, NH3-N, TN, TP, and FC) covering eight monitoring stations and 7248 monthly observations was used. The results indicated that Pukou section had the worst water quality among the eight monitoring stations, and TN was the most serious water quality index. Both the APCS-MLR and PMF models identified agricultural nonpoint source pollution, urban nonpoint source pollution and rural domestic pollution, and meteorological factors. The sum of these three sources was very close, accounting for 60% and 58%, respectively. The APCS-MLR results demonstrated that for EC, COD, and NH3-N, the major pollution sources were urban nonpoint sources and rural domestic pollution. The major contamination source of TN was agricultural nonpoint source pollution (30.4%). Meanwhile, the major pollution sources of pH, DO, TP, and FC were unidentified factors. The PMF model identified five potential sources, and pH and DO were affected by meteorological factors. NH3-N and TP were influenced mainly by agricultural nonpoint source pollution. Atmospheric deposition was the major pollution source (87.9%) of TN. FC was mostly derived from livestock and poultry breeding (88.3%). EC and COD were mostly affected by urban nonpoint sources and rural domestic pollution. Therefore, receptor models can help managers identify the major sources of pollution in watersheds, but the major factors affecting different pollutants need to be supplemented by other methods.

Brand story, as a communication tool of brand's core values, is proven to be effective in building consumers' trust and loyalty. This paper focuses on the story's protagonist identification and story's audience segmentation problem of consumers' brand storytelling, so as to support managers making brand marketing decisions. After building the scale space signature that characterized the story feature, an object pair scale transformation mechanism (Object Pair-ST) is proposed, which enables to realize the collaborative transformation of the encoded scale feature of both story's protagonists and audiences. According to the Object Pair-ST mechanism, a two-stage variable-scale clustering method (Two-stage-VSC) is put forward. The method could obtain the matched story clues (including the elements of plots, characters, causality and chronology) for every audience cluster as well as the matching degree, which are ordered by the hotness degree of consumers' interest. Experiments collected 11877 real review data on the 2022 first sales platform of time-honored enterprises, Tmall. The comparative experimental results indicate that for various types of brands, the proposed method Two-stage-VSC could always keep the granular deviation of each audience cluster at a low level while accurately identifying its protagonist cluster's story clues with the highest matching degree.

In the context of defect detection in high-speed railway train wheels, particularly in ultrasonic-testing B-scan images characterized by their small size and complexity, the need for a robust solution is paramount. The proposed algorithm, UT-YOLO, was meticulously designed to address the specific challenges presented by these images. UT-YOLO enhances its learning capacity, accuracy in detecting small targets, and overall processing speed by adopting optimized convolutional layers, a special layer design, and an attention mechanism. This algorithm exhibits superior performance on high-speed railway wheel UT datasets, indicating its potential. Crucially, UT-YOLO meets real-time processing requirements, positioning it as a practical solution for the dynamic and high-speed environment of railway inspections. In experimental evaluations, UT-YOLO exhibited good performance in best recall, mAP@0.5 and mAP@0.5:0.95 increased by 37%, 36%, and 43%, respectively; and its speed also met the needs of real-time performance. Moreover, an ultrasonic defect detection data set based on real wheels was created, and this research has been applied in actual scenarios and has helped to greatly improve manual detection efficiency.

Through comprehensively applying geological and geophysical data, as well as core and thin section observation, the characteristics of reservoirs and fractures in the second member of the Xujiahe Formation (hereinafter referred to as Xu2 Member) in the Yuanba area, northern Sichuan Basin, were studied. Combined with the analysis of the main controlling factors of production capacity, the types and characteristics of the sweet spots in the tight sandstone gas reservoir were determined. The evaluation standards and geological models of the sweet spots were established. The results are as follows: (1) There are bedding-parallel fracture-, fault-induced fracture-, and pore-dominated sweet spots in the tight sandstone gas reservoirs of the Xu2 Member. (2) The bedding parallel fracture-dominated sweet spots have developed in quartz sandstones with well-developed horizontal fractures and micro-fractures. They are characterized by high permeability and high gas output during production tests. This kind of sweet spots is thin and shows a limited distribution. Their logging responses show extremely low gamma-ray (GR) values and medium-high AC values. Moreover, the bedding parallel fracture-dominated sweet spots can be mapped using seismic methods. (3) The fault-induced fracture-dominated sweet spots have well-developed medium- and high-angle shear fractures. Their logging responses show an increase in peaks of AC values and total hydrocarbon content and a decrease in resistivity. Seismically, the areas with well-developed fault-induced fracture-dominated sweet spots can be effectively mapped using the properties such as seismic entropy and maximum likelihood. (4) The pore-dominated sweet spots are developed in medium-grained feldspathic litharenites with good reservoir properties. They are thick and widely distributed. (5) These three types of sweet spots are mainly determined by sedimentation, diagenesis, and tectonism. The bedding parallel fracture-dominated sweet spots are distributed in beach-bar quartz sandstones on the top of the 1st sand layer group in the Xu2 Member, which develops in a shore-shallow lake environment. The fault-induced fracture-dominated sweet spots mainly occur near faults. They are increasingly developed in areas closer to faults. The pore-dominated sweet spots are primarily distributed in the 2nd and 3rd sand layer groups, which lie in the development areas of distributary channels near provenances at western Yuanba area. Based on the geological and seismic data, a comprehensive evaluation standard for these three types of sweet spots of the tight sandstone reservoirs in the Xu2 Member has been established, which, on the one hand, lays the foundation for the development and evaluation of the gas reservoir, and on the other hand, deepens the understanding of sweet spot in the tight sandstone gas reservoirs.

Oiltea-camellia (C. oleifera) is a widely cultivated woody oil crop in Southern China and Southeast Asia. The genome of oiltea-camellia was very complex and not well explored. Recently, genomes of three oiltea-camellia species were sequenced and assembled, multi-omic studies of oiltea-camellia were carried out and provided a better understanding of this important woody oil crop. In this review, we summarized the recent assembly of the reference genomes of oiltea-camellia, genes related to economic traits (flowering, photosynthesis, yield and oil component), disease resistance (anthracnose) and environmental stress tolerances (drought, cold, heat and nutrient deficiency). We also discussed future directions of integrating multiple omics for evaluating genetic resources and mining key genes of important traits, and the application of new molecular breeding and gene editing technologies to accelerate the breeding process of oiltea-camellia.

It is challenging to investigate the transport and dispersion of contaminants in river confluence areas due to the complex flow dynamics. In recent studies on the flow dynamics in river confluence areas, it has been revealed that changes in inflow conditions (discharge ratio, width-depth ratio and concentration difference) can greatly influence pollutant diffusion. In this study, an asymmetric confluence-type river is modeled by a three-dimensional hydrodynamic water quality model, and three hydrodynamic scenarios are numerically simulated. The results show that a higher discharge ratio and width-depth ratio led to an increase in the lateral diffusion area of pollutants, deviation in the trajectory line of the mixing interface towards the opposite bank of the interchange, and an increase in the mixing rate of pollutants. For R = 0.267 and b/h = 3.75, the pollutants at the bottom are completely mixed in the exit end section. However, the difference in the pollutant concentration slightly affects the area, length and shape of the pollutant dispersion zone and notably affects only the concentration in each section.

Although the Triassic Xujiahe Formation in Yuanba area of northern Sichuan has a large amount of resources, the proportion of proved reserves to the amount of resources is low. Production of most of the pilot production wells is high at the initial stage, but it declines rapidly, mainly due to tight rock properties and strong heterogeneity of Xujiahe Formation. Thus, it is important to investigate the distribution of high-quality reservoirs. The Member 3 of Xujiahe Formation develops sandstone interbedded with mudstone, and its lateral thickness of different sandstone groups varies greatly, so it is a challenge to identify appropriate time windows for seismic attribute analysis, thus, so the prediction effect of the glutenite in the Member 3 of Xujiahe Formation. In this study, through analysis of core data and logging facies, single-well sedimentary microfacies subdivision is carried out. Single-well lithological interpretation is also studied with application of lithological identification technique. Then, lithological associations and its seismic responses at the top and bottom interfaces of each sandstone group in the Member 3 of Xujiahe Formation are well investigated, The lithological association with the glutenite above and the mudstone below the stratigraphic interface respectively, corresponds to a trough in seismic profiles; in this case, when extracting the seismic attributes of sandstone groups above the stratigraphic interface, only the difference of between drilling horizons and seismic horizons needs to be considered. The lithological association with the mudstone above and glutenite below the stratigraphic interface respectively, corresponds to a peak in seismic profiles; in this case, when extracting the seismic attributes of sandstone groups above the stratigraphic interface, influence of which should be eliminated; thus, a method of time window determination for seismic attribute extraction is established. Among amplitude, frequency, and phase attributes, the total peak energy has the good correlation with thickness of the glutenite at well sites and it also can reflect the distribution of distributary channels well. The results show that belt-like glutenite in each sandstone group in the Member 3 of Xujiahe Formation extends in a NW-SE direction and thins out toward the southeast, and the distribution of glutenite has certain inheritance in the longitudinal direction, the glutenite in the third sandstone group in the Member 3 of Xujiahe Formation (TX33) and the first sandstone group in the Member 3 of Xujiahe Formation (TX31) are both developed.

IntroductionThe involvement of osteocalcin in the regulation of glucose tolerance in humans is controversial. We utilized a novel and practical insulin resistance surrogate, the triglyceride-glucose (TyG) index, to investigate the association between serum osteocalcin and insulin resistance in men with type 2 diabetes (T2D).MethodsThis was a retrospective cross-sectional study that included 667 male patients suffering from T2D, with measurements of N-terminal mid-fragment of osteocalcin (N-MID), triglycerides (TG), fasting blood glucose (FBG) and C-peptide collected on the same day. We used the TyG index and HOMA-IR as surrogate measures for insulin resistance. Binary logistic regression models that adjust the sociodemographic characteristics and metabolism-related factors were used to assess the associations between osteocalcin and insulin resistance. Restricted cubic spline (RCS) analysis was used to test the potential non-linear relationship between N-MID and the risk of severe insulin resistance. Subgroup analysis evaluated the robustness of the association.ResultsN-MID was correlated with the level of insulin resistance when quantified by the TyG index in unadjusted and adjusted binary logistic regression models (all p < 0.05), but the relationship was not observed when assessed by HOMA-IR (all p > 0.05). RCS model further confirmed that the association between N-MID and the severe insulin resistance measured by the TyG index was non-linear (P = 0.047). Subgroup analysis showed that the association was detected only in younger patients with lower BMI and poorer glycemic control, without hypertension or smoking.ConclusionsOsteocalcin was inversely associated with the TyG index in men with T2D.

Since the 1990s, global warming has substantially affected the dynamic responses of forest ecosystems to drought by altering tree growth and ecosystems carbon cycling. The Loess Plateau is a typical vegetation recovery region with an arid and semiarid climate. However, the responses of the vegetation in this region to drought have not been fully studied. We therefore aimed to characterize these responses in tree-ring samples, which were obtained by drilling tree cores with a growth cone, for establishing the tree-ring width chronologies. In addition, we investigated the main factors controlling the radial growth of Chinese pine (Pinus tabulaeformis) and its dynamic responses to drought in the Loess Plateau using correlation analysis. Our results show that radial growth in Chinese pine had a positive correlation with precipitation during the last growing season, pre-growing season, and entire current growing season. The correlation between the radial growth and temperature was inconsistent between different sampling sites and time periods. These suggest that precipitation was more likely to affect radial vegetation growth than temperature. Moreover, the drought indices calculated using data before the year 2000 more accurately reflected the vegetation drought situation in Loess Plateau than data from the last 20 years. The drier the place, the more accurately the drought indices represented the responses of vegetation to drought. However, these indices cannot satisfactorily capture the drought responses of vegetation in wet regions. Furthermore, the PDSI was more accurate than the SPEI at capturing the effects of drought on radial vegetation growth. Understanding the response mechanism of the radial growth of Chinese pine to drought can provide theoretical support for ecological protection, forest management, and ecological construction under climate change.

Accurately quantifying the spatiotemporal nutrient loads from nonpoint sources (NPSs) is still difficult due to the high spatial heterogeneity and temporal variability in rainfall-runoff processes. The export coefficient model (ECM) is a widely used tool to estimate the NPS nutrient load on an annual time scale, but it hardly considers the spatiotemporal distribution of the export coefficient. To address this issue, the ECM is improved by incorporating a grid-based rainfall-runoff module together with land-use data and analysing the spatiotemporal characteristics of the NPS nutrient loads in Yancheng city located in eastern China. Moreover, the effect of NPS nutrient loads on river water quality is assessed using a conceptual model. The results indicate that the improved ECM can simulate reasonable spatiotemporal variation in NPS nutrient loads, and the relative error in the total phosphorus (TP) load is less than 17.14 %. Precipitation has a considerable influence on both the total nitrogen (TN) and TP loads, which varied greatly from 1990 to 2018, with average annual values of 30.57 × 103 tons and 2.44 × 103 tons, respectively. The spatial distributions of the TN and TP loads were greatly affected by land-use patterns, and bodies of water and rural land had relatively high load intensities. Both TN and TP concentrations in the river exceeded the national standard, but TP was significantly reduced in the past 10 years, while TN was still at high level. This study provides a reference for reducing the river pollution in Yancheng city, especially by identifying pollution sources requiring priority control.

Based on the social interdependence theory, we proposed that the distributive justice climate affects virtual team performance via high-quality relationships, and then we investigated the boundary effect of team proactive personality. The data used in this study were collected in China, including 327 virtual team members that belonged to 75 teams. The following results are obtained: (1) Distributive justice climate and high-quality relationships have significant positive effects on virtual team performance. (2) High-quality relationships mediate the relationship between the distributive justice climate and virtual team performance. (3) Team proactive personality strengthens the direct effect of the distributive justice climate on high-quality relationships. (4) Team proactive personality strengthens the indirect effect of the distributive justice climate on virtual team performance through high-quality relationships. These empirical results have important theoretical significance for team climate construction, personnel selection, and team performance promotion.

Ventilation Corridor is an important technique to mitigate local climate problems. Evaluating the benefits of ventilation corridors has gradually become the standard of its construction in cities. Previous studies have often analyzed the volume of ventilation from the perspective of geometric morphology, while there is a lack of discussion on its performance. Therefore, this study innovatively combined the roughness length (Z0), 3-dimensional aerodynamics parameter, with NDVI and M-NDWI, 2-dimensional indices of land cover through the combination of remote sensing inversion and urban morphology analysis. Compared with the traditional ventilation method, the composite indicator could not only guarantee ventilation volume, but also consider the cooling benefits brought by waters and vegetated areas. In detail, this study was contextualized in four districts in the city core of Hefei, Anhui Province, China. Based on the method of least cost path, we set up four different schemes for comparison, including roughness, roughness ​+ ​vegetated areas, roughness ​+ ​waters, and roughness ​+ ​vegetated areas ​+ ​waters. Comparative results were effectively verified through land surface temperature retrieved from the Landsat Thematic Mapper. Results were as follows: (1) Different scenarios exerted different impacts on urban ventilation. Of the four regions, the ventilation corridor that only considers Z0 presented the highest average land surface temperature. (2) The general temperature of ventilation corridors along the path of Z0 ​+ ​NDVI ​+ ​M-NDWI was respectively 34.11 ​°C, 31.73 ​°C, 34.80 ​°C, and 34.93 ​°C, presenting a lowest temperature compared with other scenarios of the same region. (3) Among the four regions, ventilation corridors constructed based on the path of Z0 ​+ ​M-NDWI presented a slightly lower temperature that those based on Z0+NDVI, with respective data of 34.22 ​°C ​

Effective chimeric antigen receptor (CAR)-T cell therapy is dependent on optimal cell culture methods conducive to the activation and expansion of T cells ex vivo, as well as infection with CAR. Media formulations used in CAR-T cell manufacturing have not been optimized for gene delivery, cell expansion, and overall potency. Bioactive components and derivatives that support the generation of functionally-competent T cell progeny with long-lasting persistence are largely undefined. Current media formulations rely on fetal bovine serum (FBS) or human serum (HS), which suffer from a lack of consistency or supply issues. We recognize that components of blood cellular fractions that are absent in serum may have therapeutic value. Here we investigate whether a concentrated growth factor extract, purified from human transfusion grade whole blood fractions, and marketed as PhysiologixTM xeno-free (XF) hGFC (Phx), supports CAR-T cell expansion and function. We show that Phx supports T cell proliferation in clinical and research-grade media. We also show that Phx treatment enhances lentiviral-mediated gene expression across a wide range of multiplicity of infections (MOIs). We compared the ability of anti-GD-2 CAR-T cells expanded ex vivo in medium conditioned with either Phx or HS to clear tumor burden in a human xenograft model of neuroblastoma. We show that T cells expanded in Phx have superior engraftment and potency in vivo, as well as CAR-induced cytolytic activity in vitro. Metabolomic profiling revealed several factors unique to Phx that may have relevance for CAR-T cell preclinical discovery, process development, and manufacturing. In particular, we show that carnosine, a biogenic amine modestly enriched in Phx relative to HS, enhances lentiviral gene delivery in activated T cells. By limiting extracellular acidification, carnosine enhances the metabolic fitness of T cells, shifting their metabolic profile from an acidic, stressed state toward an oxidative, energetic state. These findings are very informative regarding potential derivatives to include in medium customized for gene delivery and overall potency for T cell adoptive immunotherapies.

In this study, the authors aim to solve the time series prediction problem through pre-predicting multiple influence factors of the target sequence. Focusing on two pre-prediction approaches of influence factors (i.e., factors driven approach and time driven approach), we propose a time series prediction method based on the least squares support vector machine and grey model (GM-LSSVM). This method could improve the prediction precision of the target time series by differentiating the data characteristics of influence factors. A case study is put forward to predict China's economy from the perspective of system innovation and technological innovation. We selected public statistics data from 2005 to 2014 from the national bureau. The numerical experiment results illustrate that the accuracy of the GM-LSSVM is able to reach 95%, which proves the effectiveness of our proposed method in practice.

Two [RuCl(Qn)(Pro)NO] (Qn = 8-quinolinol, Pro = L-proline) isomer complexes, in which the O atom of L-proline coordinated trans to NO (Complex a) and the O atom of 8-quinolinol coordinated trans to NO (Complex b), were synthesized. Their structures were identified with X-ray diffraction and compared with optimized geometries by DFT calculations at the B3LYP level. The electronic absorption and IR spectroscopy of the isomers were experimentally measured and theoretically assigned, the front molecular orbitals were analyzed. Moreover, the photodynamic reaction for the isomers was investigated with time resolved FTIR spectroscopy, photo induced NO release of the isomers were observed. The effect of ligands and configuration on photodynamic properties of the isomers was discussed. The structural and dynamic analyses combined with DFT calculations provide insights into the physicochemical properties and reactivity of the isomeric complexes, which makes a foundation for their potential applications.

Customers' demands have become more dynamic and complicated owing to the functional diversity and lifecycle reduction of products which pushes enterprises to identify the real-time needs of distinct customers in a superior way. Meanwhile, social media turned as an emerging channel where customers often spontaneously can express their perceptions and thoughts about products promptly. This paper examines the customer satisfaction identification and improvement problem based on social media mining. First, we proposed the public opinion sensitivity index (POSI) to uncover target customers from extensive short-textual reviews. Subsequently, we presented a customer segmentation approach based on the sentiment analysis and the variable-scale clustering (VSC). The approach is able to get several customer clusters with the same satisfaction level where customers belonging to each cluster have similar interests. Finally, customer-centered marketing strategies and customer difference marketing campaigns are planned under the shadow of customer segmentation results. The experiments illustrate that our proposed method can support marketing decision marketing in practice that enriches the intention of the current customer relationship management.

Social media has already become one of the mainstream enterprise marketing channels recently. That consists of various media elements, such as text, picture, and even newly developed instant music video etc. Although several text or picture mining techniques could be directly utilized to analyse online user comments, few researches focus on how to improve the marketing performance of social media platforms through a multimedia approach. Therefore, this paper studies the social media marketing problem of user generated instant music video. A hybrid variable-scale clustering algorithm (HVSC) is proposed to analyse user feature through both textual and video content. Combining with the information dissemination characteristics of social media platforms, we also put forth a marketing strategy that intensively enlarges the transmission audience of influential UGC videos. Experiment results show that the HVSC is able to support managers to discover the target potential customer base of each UGC video following their music preference and current interest/concerns. Finally, according to the content relevance and customer influence, the video producers’ incentive mechanism is further discussed.

Multi-attribute decision making (MADM) always suffers from the result inconsistency and computational complexity problem, due to numbers of redundant and relational attributes (indexes) of the initial evaluation index system. Therefore, this paper studies the index system (IS) reduction problem through selecting the most representative indicator from each index subsystem after the IS structure partition. First, we propose and demonstrate the Index Subsystem Judgement theorem to improve the efficiency of the classic system structure partition algorithm. Second, an algorithm of index system reduction based on the index similarity (ISRS) is put forward. The ISRS is able to reduce the index quantity while still keeping the index meaning. Third, we define the direction loss rate to measure the evaluation ability loss of the IS during reduction. The algorithm is tested for a synthetic dataset to compare the proposed ISRS with different index reduction algorithms, followed by an extensive experimentation with a real-world financial dataset. Experiment results illustrate that our proposed method is able to obtain more accessible and available reduction results in practice.

Business and data understanding, that aims to identify multiple mining tasks, is the most primary phase in planning a practical data mining project. However, traditional tasks determination problem could only be solved by experienced analysts, which suffers from high communication cost and low efficiency. In this paper, we study the automatic task discovery method following the problem solving theory. First, we establish the concept network (CN) model to represent human knowledge and experience in the problem solving process. Then, we propose and demonstrate the structure of two major mining tasks (clustering and classification) in a CN. Finally, a data mining tasks discovery method (DMTD) is put forward, followed by two analysis subject evaluation algorithms. Experiment results illustrate that the DMTD is able to discover all the potential mining tasks from a predefined concept network, filtered by the important or interesting analysis subjects. Moreover, these tasks defined by the DMTD are proven to be available and valuable by fifty published papers.

Ventilation corridors in cities can decrease air pollution and alleviate heat island problems but there remains a need to fully assess their effectiveness. Few urban managers have been able to take city-scale approaches to the construction of urban ventilation corridors. This study aimed to introduced the Ventilation Corridor Planning (VCP) model, which is a multi-criteria evaluation method combined with a geographical information system (GIS) to determine where the ventilated environment is most appropriate. Specifically, the VCP model took Bozhou, China as the research object and contained two scales, including mesoscale and local scale. In mesoscale scale, we got three outputs to build urban ventilation corridors, including 1) background wind environment, 2) ventilation potential, 3) heat island intensity. In local scale, we used traditional computational fluid dynamics (CFD) model to verify the impact of VCP criteria. The results revealed that compared with the traditional CFD model, the proposed VCP model has advantages in establishing a comprehensive evaluation standard. In addition, the application of VCP model in macro and micro also enhances the efficiency of ventilation corridor construction. Overall, this study introduced a effective modeling method to urban ventilation corridors planning, and provide a way to study the urban climate.

The title polymer [HCO2Sn(C6H5)3]n was isolated by serendipitous from reaction in ethanol 96 % between bis-(monocyclohexyl ammonium) 2-carboxyethyl hydrogen phosphonate, {(CyNH3)2[CO2CH2CH2PO2(OH)]} and triphenyltin chloride, SnPh3Cl. The polymer crystallizes in the monoclinic space group P21/n with Z = 4, a = 14.7091 (18) Å, b = 15.4133 (19) Å, c = 14.7639 (18) Å, β = 94.242 (2)° and V = 3338.0 (7) Å3. The structure consists of an infinite chain propagating along the crystallographic a-axis direction with bidentate formate anions. The O2SnC3 core is organized into a trans-trigonal bipyramidal like-arrangement.

Abstract Background Imbalances in circulating T lymphocytes play critical roles in the pathogenesis of hypertension-mediated inflammation. Connexins (Cxs) in immune cells are involved in the maintenance of homeostasis of T lymphocytes. However, the association between Cxs in peripheral blood T lymphocytes and hypertension-mediated inflammation remains unknown. This study was designed to investigate the role of Cxs in T lymphocytes in hypertension-mediated inflammation in spontaneously hypertensive rats (SHRs). Methods The systolic blood pressure (SBP) in Wistar-Kyoto (WKY) rats and SHRs was monitored using the tail-cuff method. The serum cytokine level was determined using ELISA. The proportions of different T-lymphocyte subtypes in the peripheral blood, the expressions of Cx40/Cx43 in the T-cell subtypes, and the gap junctional intracellular communication (GJIC) of peripheral blood lymphocytes were measured using flow cytometry (FC). The accumulations of Cx40/Cx43 at the plasma membrane and/or in the cytoplasm were determined using immunofluorescence staining. The in vitro mRNA levels of cytokines and GJIC in the peripheral blood lymphocytes were respectively examined using real-time PCR and FC after treatment with Gap27 and/or concanavalin A (Con A). Results The percentage of CD4+ T cells and the CD4+/CD8+ ratio were high, and the accumulation or expressions of Cx40/Cx43 in the peripheral blood lymphocytes in SHRs were higher than in those of WKY rats. The percentage of CD8+ and CD4+CD25+ T cells was lower in SHRs. The serum levels of IL-2, IL-4 and IL-6 from SHRs were higher than those from WKY rats, and the serum levels of IL-2 and IL-6 positively correlated with the expression of Cx40/Cx43 in the peripheral blood T lymphocytes from SHRs. The peripheral blood lymphocytes of SHRs exhibited enhanced GJIC. Cx43-based channel inhibition, which was mediated by Gap27, remarkably reduced GJIC in lymphocytes, and suppressed IL-2 and IL-6 mRNA expressions in Con A stimulated peripheral blood lymphocytes. Conclusions Our data suggest that Cxs may be involved in the regulation of T-lymphocyte homeostasis and the production of cytokines. A clear association was found between alterations in Cxs expression or in Cx43-based GJIC and hypertension-mediated inflammation.

Two light-activated NO donors [RuCl(qn)(Lbpy)(NO)]X with 8-hydroxyquinoline (qn) and 2,2′-bipyridine derivatives (Lbpy) as co-ligands were synthesized (Lbpy1 = 4,4′-dicarboxyl-2,2′-dipyridine, X = Cl− and Lbpy2 = 4,4′-dimethoxycarbonyl-2,2′-dipyridine, X = NO3−), and characterized using ultraviolet–visible (UV-vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (1H NMR), elemental analysis and electrospray ionization mass spectrometry (ESI-MS) spectra. The [RuCl(qn)(Lbpy2)(NO)]NO3 complex was crystallized and exhibited distorted octahedral geometry, in which the Ru–N(O) bond length was 1.752(6) Å and the Ru–N–O angle was 177.6(6)°. Time-resolved FT-IR and electron paramagnetic resonance (EPR) spectra were used to confirm the photoactivated NO release of the complexes. The binding constant (Kb) of two complexes with human serum albumin (HSA) and DNA were quantitatively evaluated using fluorescence spectroscopy, Ru-Lbpy1 (Kb~106 with HSA and ~104 with DNA) had higher affinity than Ru-Lbpy2. The interactions between the complexes and HSA were investigated using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and EPR spectra. HSA can be used as a carrier to facilitate the release of NO from the complexes upon photoirradiation. The confocal imaging of photo-induced NO release in living cells was successfully observed with a fluorescent NO probe. Moreover, the photocleavage of pBR322 DNA for the complexes and the effect of different Lbpy substituted groups in the complexes on their reactivity were analyzed.

Power grid planning is an important part in the development of power grid. With the rapid development of urban construction and electricity demand in recent years, electric power construction is faced with more kinds of pressure, such as land resources/environmental protection. In this paper, the difficulty of power grid planning is increasing rapidly. How to build a distribution network that the load and the urban construction can develop concordantly has become a key issue to be solved in the future. The traditional methods of distribution network planning focus on the grasp of the overall, whereas lack adequate consideration of the spatial distribution of load and planning of urban development. Thus, this paper proposes a method based on spatial load forecasting of urban distribution network planning g, and fully considers the load distribution of the space characteristics and urban construction. This method can make up for the defects of traditional methods effectively, thus has certain value and reference significance.

ABSTRACTHMOX1 is an important gene in biosynthesis of the eggshell pigment of blue eggs. Previous studies found that HMOX1 is highly expressed in the shell gland of hens laying blue eggs (BlueH) compared with hens laying brown eggs (BrownH); however, the reasons for the differential expression are unclear. In this study five single nucleotide polymorphism (SNP) in HMOX1 were genotyped in 111 BlueH and 115 BrownH. The association of haplotypes of these SNP with the blue egg phenotype was tested. Haplotype-specific expression of HMOX1 was detected in the shell gland. The interaction of sequence variants and transcription factors was analyzed using electrophoretic mobility shift assay (EMSA). A TG haplotype covering upstream 1.4 kb region of HMOX1 was significantly associated with blue eggs (p

Inflammation has been shown to play an important role in the mechanisms involved in the pathogenesis of hypertension. Connexins (Cxs)-based gap junction channels (GJCs) or hemichannels (HCs) are involved in the maintenance of homeostasis in the immune system. However, the role of Cx43-based channels in T-lymphocytes in mediating the immune response in essential hypertension is not fully understand. The present study was designed to investigate the role of Cxs-based channels in T lymphocytes in the regulation of hypertension-mediated inflammation. The surface expressions of T lymphocyte subtypes, Cx40/Cx43, and inflammatory cytokines (IFN-γ (interferon-gamma) and TNF-ɑ (tumor necrosis factor alpha)) in T cells, as well as gap junction communication of peripheral blood lymphocytes from essential hypertensive patients (EHs) and normotensive healthy subjects (NTs) were detected by flow cytometry. Expression levels and phosphorylation of Cx43 protein in peripheral blood lymphocytes of EHs and NTs were analyzed by Western blot. The proliferation rate of peripheral blood mononuclear cells (PBMCs) after treatment with a Cxs inhibitor was examined by a CCK-8 assay. The levels of inflammatory cytokines were detected using ELISA. Within the CD3+ T cell subsets, we found a significant trend toward an increase in the percentage of CD4+ T cells and CD4+/CD8+ ratio as well as in serum levels of IFN-γ and TNF-ɑ in the peripheral blood of EHs compared with those in NTs. Moreover, the peripheral blood lymphocytes of EH patients exhibited enhanced GJCs formation, increased Cx43 protein level and Cx43 phosphorylation at Ser368, and a significant increase in Cx40/Cx43 surface expressions levels in CD4+ or CD8+ T lymphocytes. Cx43-based channel inhibition by a mimetic peptide greatly reduced the exchange of dye between lymphocytes, proliferation of stimulated lymphocytes and the pro-inflammatory cytokine levels of EHs and NTs. Our data suggest that Cx40/Cx43-based channels in lymphocytes may be involved in the regulation of T lymphocyte proliferation and the production of pro-inflammatory cytokines, which contribute to the hypertensive inflammatory response.

Jointly influenced by natural factors and artificial protection measures in recent years, the vegetation coverage of the Loess Plateau has significantly increased. However, extensive vegetation recovery can result in massive water consumption and a severe soil water deficit, which poses a great threat to the sustainable development of the regional ecological system. Maintaining the balance between precipitation and water consumption is an important foundation of ecological security in the Loess Plateau. Based on this, the present study used the GRACE (Gravity Recovery and Climate Experiment) gravity satellite data to simulate the annual actual water consumption from 2003 to 2014 and to analyze the temporal and spatial evolution of the regional precipitation and the actual evapotranspiration (AET). This study also applied the newly developed rainwater utilization potential index (IRUP) to quantify the sustainability of the water balance in the Loess Plateau. The spatial-temporal patterns of precipitation, potential evapotranspiration, and AET from 2003 to 2014 in the Loess Plateau were all analyzed in this study. Based on the results, the annual average precipitation (AAP) and AET in the entire Loess Plateau had significant increasing trends. The analysis of the spatial distribution reveals that the AET was decreasing from the southeast to the northwest in the Loess Plateau. However, the average values of potential evapotranspiration did not obviously change. Based on the estimated AET result, it was determined that the average IRUP had an increasing trend. The increase in the IRUP is due to an increased rate of precipitation that is statistically higher than that of the AET. Consequently, the Loess Plateau experienced a wetting trend during the period of 2003⁻2014, especially after the Grain for Green project was implemented. The results in this paper were proven by using three different depths of ERA-Interim (a global atmospheric reanalysis product created by the European Centre for Medium-Range Weather Forecasts) soil water content data from the same period and the observed runoff data from 18 different hydrological sites. Consequently, it seems that the vegetation could maintain a sustainable growth with the implementation of the Grain for Green Project.