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This article describes the application of the concept of geographic profiling in hunting a serial child rapist in Kasur, Pakistan. It also discusses, how DNA became the prime witness against the serial rapist in the court of law. In January 2018, the blind rape and murder case of Zainab Amin hit the headlines. Following autopsy and the subsequent forensic examination, the only piece of evidence, the agencies had, was the DNA profile of the perpetrator and the information that the source of DNA profile is a serial child rapist, involved in at least seven more cases. The analysis of all crime sites and the distance between them strongly suggested that the offender most likely was a local resident. Mass DNA screening in the target region was conducted by CSI teams of Punjab forensic science agency. The DNA matched with suspect number 814 who later confessed all his crimes. In Polygraph examination, the offender revealed his modus operandi which was in line with the hypotheses made during the geographic profiling of the crime scenes. Thus, geographic profiling proved to be a very useful investigative tool in predicting the probable location of the criminal involved in a series of crimes.

To evaluate factors associated with ICU delirium in patients who underwent damage control laparotomy (DCL), with the hypothesis that benzodiazepines and paralytic infusions would be associated with increased delirium risk. We also sought to evaluate the differences in sedation practices between trauma (T) and non-trauma (NT) patients. We reviewed retrospective data from 15 centers in the EAST SLEEP-TIME registry admitted from January 1, 2017 to December 31, 2018. We included all adults undergoing DCL, regardless of diagnosis, who had completed daily Richmond Agitation Sedation Score (RASS) and Confusion Assessment Method-ICU (CAM-ICU). We excluded patients younger than 18 years, pregnant women, prisoners and patients who died before the first re-laparotomy. Data collected included age, number of re-laparotomies after DCL, duration of paralytic infusion, duration and type of sedative and opioid infusions as well as daily CAM-ICU and RASS scores to analyze risk factors associated with the proportion of delirium-free/coma-free ICU days during the first 30 days (DF/CF-ICU-30) using multivariate linear regression. A 353 patient subset (73.2% trauma) from the overall 567-patient cohort had complete daily RASS and CAM-ICU data. NT patients were older (58.9 ± 16.0 years vs 40.5 ± 17.0 years [p

Introduction: The present study was conducted to validate the reduced PCR reaction volume of Globalfiler™ amplification kit for the amplification of non-quantified DNA from standard reference sampl...

Background: Global Filer PCR Amplification Kit is a 6-dye amplification assay, which amplifies 24 genetic loci simultaneously in a single reaction. The amplification system encompasses both Combine...

Damage control laparotomy (DCL) is used for both traumatic and non-traumatic indications. Failure to achieve primary fascial closure (PFC) in a timely fashion has been associated with complications including sepsis, fistula, and mortality. We sought to identify factors associated with time to PFC in a multicenter retrospective cohort.We reviewed retrospective data from 15 centers in the EAST SLEEP-TIME registry, including age, comorbidities (Charlson Comorbidity Index [CCI]), small and large bowel resection, bowel discontinuity, vascular procedures, retained packs, number of re-laparotomies, net fluid balance after 24 h, trauma, and time to first takeback in 12-h increments to identify key factors associated with time to PFC.In total, 368 patients (71.2% trauma, of which 50.6% were penetrating, median ISS 25 [16, 34], with median Apache II score 15 [11, 22] in non-trauma) were in the cohort. Of these, 92.9% of patients achieved PFC at 60.8 ± 72.0 h after 1.6 ± 1.2 re-laparotomies. Each additional re-laparotomy reduced the odds of PFC by 91.5% (95%CI 88.2-93.9%, p 0.001). Time to first re-laparotomy was highly significant (p 0.001) in terms of odds of achieving PFC, with no difference between 12 and 24 h to first re-laparotomy (ref), and decreases in odds of PFC of 78.4% (65.8-86.4%, p 0.001) for first re-laparotomy after 24.1-36 h, 90.8% (84.7-94.4%, p 0.001) for 36.1-48 h, and 98.1% (96.4-99.0%, p 0.001) for 48 h. Trauma patients had increased likelihood of PFC in two separate analyses (p = 0.022 and 0.002).Time to re-laparotomy ≤ 24 h and minimizing number of re-laparotomies are highly predictive of rapid achievement of PFC in patients after trauma- and non-trauma DCL.2B.

To evaluate factors associated with ICU delirium in patients who underwent damage control laparotomy (DCL), with the hypothesis that benzodiazepines and paralytic infusions would be associated with increased delirium risk. We also sought to evaluate the differences in sedation practices between trauma (T) and non-trauma (NT) patients.We reviewed retrospective data from 15 centers in the EAST SLEEP-TIME registry admitted from January 1, 2017 to December 31, 2018. We included all adults undergoing DCL, regardless of diagnosis, who had completed daily Richmond Agitation Sedation Score (RASS) and Confusion Assessment Method-ICU (CAM-ICU). We excluded patients younger than 18 years, pregnant women, prisoners and patients who died before the first re-laparotomy. Data collected included age, number of re-laparotomies after DCL, duration of paralytic infusion, duration and type of sedative and opioid infusions as well as daily CAM-ICU and RASS scores to analyze risk factors associated with the proportion of delirium-free/coma-free ICU days during the first 30 days (DF/CF-ICU-30) using multivariate linear regression.A 353 patient subset (73.2% trauma) from the overall 567-patient cohort had complete daily RASS and CAM-ICU data. NT patients were older (58.9 ± 16.0 years vs 40.5 ± 17.0 years [p 0.001]). Mean DF/CF-ICU-30 days was 73.7 ± 96.4% for the NT and 51.3 ± 38.7% in the T patients (p = 0.030). More T patients were exposed to Midazolam, 41.3% vs 20.3% (p = 0.002). More T patients were exposed to Propofol, 91.0% vs 71.9% (p 0.001) with longer infusion times in T compared to NT (71.2 ± 85.9 vs 48.9 ± 69.8 h [p = 0.017]). Paralytic infusions were also used more in T compared to NT, 34.8% vs 18.2% (p 0.001). Using linear regression, dexmedetomidine infusion and paralytic infusions were associated with decreases in DF/CF-ICU-30, (- 2.78 (95%CI [- 5.54, - 0.024], p = 0.040) and (- 7.08 ([- 13.0, - 1.10], p = 0.020) respectively.Although the relationship between paralytic use and delirium is well-established, the observation that dexmedetomidine exposure is independently associated with increased delirium and coma is novel and bears further study.

BackgroundDamage-control laparotomy (DCL) has been used for traumatic and nontraumatic indications. We studied factors associated with delirium and outcome in this population.MethodsWe reviewed DCL patients at 15 centers for 2 years, including demographics, Charlson Comorbidity Index (CCI), diagnosis, operations, and outcomes. We compared 30-day mortality; renal failure requiring dialysis; number of takebacks; hospital, ventilator, and intensive care unit (ICU) days; and delirium-free and coma-free proportion of the first 30 ICU days (DF/CF-ICU-30) between trauma (T) and nontrauma (NT) patients. We performed linear regression for DF/CF-ICU-30, including age, sex, CCI, achievement of primary fascial closure (PFC), small and large bowel resection, bowel discontinuity, abdominal vascular procedures, and trauma as covariates. We performed one-way analysis of variance for DF/CF-ICU-30 against traumatic brain injury severity as measured by Abbreviated Injury Scale for the head.ResultsAmong 554 DCL patients (25.8% NT), NT patients were older (58.9 ± 15.8 vs. 39.7 ± 17.0 years, p < 0.001), more female (45.5% vs. 22.1%, p < 0.001), and had higher CCI (4.7 ± 3.3 vs. 1.1 ± 2.2, p < 0.001). The number of takebacks (1.7 ± 2.6 vs. 1.5 ± 1.2), time to first takeback (32.0 hours), duration of bowel discontinuity (47.0 hours), and time to PFC were similar (63.2 hours, achieved in 73.5%). Nontrauma and T patients had similar ventilator, ICU, and hospital days and mortality (31.0% NT, 29.8% T). Nontrauma patients had higher rates of renal failure requiring dialysis (36.6% vs. 14.1%, p < 0.001) and postoperative abdominal sepsis (40.1% vs. 17.1%, p < 0.001). Trauma and NT patients had similar number of hours of sedative (89.9 vs. 65.5 hours, p = 0.064) and opioid infusions (106.9 vs. 96.7 hours, p = 0.514), but T had lower DF/CF-ICU-30 (51.1% vs. 73.7%, p = 0.029), indicating more delirium. Linear regression analysis indicated that T was associated with a 32.1% decrease (95% CI, 14.6%-49.5%; p < 0.001) in DF/CF-ICU-30, while achieving PFC was associated with a 25.1% increase (95% CI, 10.2%-40.1%; p = 0.001) in DF/CFICU-30. Increasing Abbreviated Injury Scale for the head was associated with decreased DF/CF-ICU-30 by analysis of variance (p < 0.001).ConclusionNontrauma patients had higher incidence of postoperative abdominal sepsis and need for dialysis, while T was independently associated with increased delirium, perhaps because of traumatic brain injury.Level of evidenceTherapeutic study, level IV.

Objective: The primary point of the current exploration was to analyze antimicrobial helplessness plans of Escherichia coli bacteremia among tumor patients, likewise to quantify peril issues additionally outcomes of multidrug-strong Escherichia coli bacteremia. Methods: Our current research was conducted at Jinnah Hospital, Lahore from March 2019 to February 2020. other than it included restorative accounts of patients through Escherichia coli bacteremia offering among March 2019 to February 2020. Various strategic decays inspect stayed rehearsed to direct issues related by development likewise 30-day humankind of multidrug-tough Escherichia coli bacteremia. Results: In all out of 1610 episodes of bacteremia, 228(36.1%) remained created through E. coli, of them 99(44.7%) remained multidrug-tough. In numerous adaptable examinations, oldness less than 19 years (acclimated probabilities extent 4.93; 94% sureness interlude 1.44-11.69), participation of predominant venous catheter (adjusted chances connection 3.13; 96% certainty break 1.05-5.34) additionally experience to piperacilin/tazobactam in 95 days before defilement (adjusted chances proportion 2.38; 96% CI 1.16-5.87) stayed perceived via self-sufficient peril issues for achievement of multidrug-versatile Escherichia coli bacteremia. The overall 35-day passing extent stayed 36.3% (81/228). Threat issues for humankind stayed concentrated upkeep part affirmation (acclimated probabilities connection 4.96; 94% certainty span 1.78-9.72) likewise intelligent neutropenia (adjusted possibilities extent 3.04; 92% CI 1.56-11.48). Conclusion: Circulation (blood) disease through multidrug-strong Escherichia coli stayed known in tumor patients. However, this stayed not the investigator of death. Keywords: Antimicrobial Powerlessness, Escherichia Coli Bacteremia, Multidrug-Safe Escherichia Coli Bacteremia.

Aim: Osteoporosis among peptic ulcer disorder stays one of challenges habitually depicted. The goal of our ebb and flow research was to control event of osteoporosis in cases by peptic ulcer infection. Methods: Our current research was conducted at Sir Ganga Ram Hospital, Lahore from March 2019 to February 2020. In our ebb and flow research study patients old enough 19 years or, more than likely extra recognized by peptic ulcer disease on past additionally then since quite a while ago settled on higher GI endoscopy, remained involved. Those patients stayed then estimated on DEXA filter moreover osteoporosis remained considered as yes anyplace here remained score of less than 3.6. Data remained showed up moreover analyzed by utilizing SPSS form 22. Results: In our flow research concentrate here stayed entire 65 patients out of which 31 remained men what's more, 33 ladies. The normal age stayed 44.24±9.14 years. The normal time of PUD stayed 4.3±0.32 years likewise normal T score stayed 2.3±0.9. Osteoporosis remained comprehended in 5 (7.68%) of patients. Osteoporosis stayed extra comprehended in ladies disturbing 04 (10.39%) patients of them singular sets (p= 0.03). Here stayed no significant modification in compatibilities of time of PUD by osteoporosis (p= 0.87). Conclusion: Osteoporosis remains newly investigated issue of peptic ulcer disease additionally isn't genuine known, however on the other hand actually existing among various patients of peptic ulcer disorder as appeared by our exploration. Key words: Recurrence, Osteoporosis, Patients, Peptic Ulcer Sickness.

Experimental design is a key ingredient of reproducible empirical research. Yet, given the increasing complexity of experimental designs, researchers often struggle to implement ones that allow them to measure their variables of interest without confounds. SweetPea is an open-source declarative language in Python, in which researchers can describe their desired experiment as a set of factors and constraints. The language leverages advances in areas of computer science to sample experiment sequences in an unbiased way. In this article, we provide an overview of SweetPea’s capabilities, and demonstrate its application to the design of psychological experiments. Finally, we discuss current limitations of SweetPea, as well as potential applications to other domains of empirical research, such as neuroscience and machine learning.

Studies of the human skin microbiome suggest that Propionibacterium acnes strains may contribute differently to skin health and disease. However, the immune phenotype and functions of T helper type 17 (Th17) cells induced by healthy (PH) versus acne (PA) skin-associated P. acnes strains are currently unknown. We stimulated peripheral blood mononuclear cells from healthy donors and observed that PA strains induce higher IL-17 levels than PH strains. We next generated PH and PA strain-specific Th17 clones and show that P. acnes strains induce Th17 cells of varied phenotype and function that are stable in the presence of IL-2 and IL-23. Although PH- and PA-specific clones expressed similar levels of LL-37 and DEFB4, only PH-specific clones secreted molecules sufficient to kill P. acnes. Furthermore, electron microscopic studies showed that supernatants derived from activated PH and not PA-specific clones exhibited robust bactericidal activity against P. acnes, and complete breaches in the bacterial cell envelope were observed. This antimicrobial activity was independent of IL-26, because both natural IL-26 released by Th17 clones and rhIL-26 lacked antimicrobial potency against P. acnes. Overall, our data suggest that P. acnes strains may differentially modulate the CD4+ T-cell responses, leading to the generation of Th17 cells that may contribute to either homeostasis or acne pathogenesis.

To analyze the clinical presentation, treatment given, and outcome of patients suffering from congenital and acquired malaria in neonatal period.Analytical study.Paediatrics Ward-2, QAMC/BVH, Bahawalpur for 02 years from October 2001 to October 2003.The study included 45 cases of neonatal malaria. Thirty cases of malaria, admitted during first ten days of life, diagnosed as congenital malaria, were kept in group A, while 15 cases admitted in the ward from the age of 11 to 28 days, labeled as acquired malaria, were named group B. The clinical features at the time of presentation were noted in each group from the charts having positive malarial parasite (M.P.) on thick and thin slides. The diagnosed cases were treated with the standard dose of chloroquine sulphate. Those patients who improved clinically as well as revealed no parasite on follow-up were labeled as chloroquine sensitive. On the other hand, patients showing poor clinical response with persistence of the parasites in the blood or initially disappearing but later again having a clinical disease with positive M.P. on follow-up, were labeled as chloroquine resistant. They were treated with quinine sulphate. Outcome was compared in both the groups regarding the pattern of chloroquine resistance and death/ survival. Data was collected on which Fischer's exact test of significance was performed to know the level of significance. P-value of0.05 was taken as statistically significant.Low birth weight, severe hemolytic anemia with history of fever in the mother were main features in group A while in group B fever, anaemia and history of blood transfusion were marked features. In group A 76.66% were caused by Plasmodium (P.) falciparum. While in group B 60% were caused by Plasmodium vivax. In group A 26.66% were chloroquine resistant while 33.65% were chloroquine resistant in group B. The mortality was 16.66% in group A and 13.33% in group B.Intrauterine growth retardation, hemolytic jaundice and history of fever in the mother in the last trimester of pregnancy in the congenital while fever, history of blood transfusion in the neonates in acquired malaria but pallor in both the groups, were important clinical features. Pattern of chloroquine resistance and mortality in both the groups was not statistically different.

Bcr-abl translocation has been found to be the hallmark of chronic myelogenous leukemia. It is present in 90-95% cases of chronic myelogenous leukemia. The present study was performed to check the frequency of Bcr-abl fusion transcripts in Pakistani chronic myelogenous leukemia patients. The frequency of Bcr-abl fusion transcript was determined by using RT-PCR. RNA was isolated from the peripheral blood of 23 CML patients using TRIzol reagent. It was reverse transcribed by using MMLV reverse transcriptase enzyme followed by RT-PCR. RT-PCR was done by using two combinations of primers (BCR-C, CA3) and (B2B, CA3). The PCR product was analysed on 3% Agarose gel. Results: All 23 samples were positive for Bcr-abl translocation. Out of 23 samples 13 (56.5%) were positive for b2a2 type transcript. B3a2 transcript was observed in 6 (26%) out of 23 samples. Other samples gave unusual bands of around 209 bp referring to fusion fragments other than b2a2 or b3a2. None of 23 samples was positive for e1a2 type transcript. In Pakistani population b2a2 type transcript is most frequent in contrast to the studies conducted on other populations. That is perhaps due to the ethnic differences.