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Non thermal plasma (NTP) is a modern technology with wide spectrum applications in different biological, and medical approaches. By releasing reactive oxygen species (ROS), it allows stimulation or inhibition of cellular processes in biological systems. This technology has been employed in the distinction of bacteria and viruses in food researches. HCV is a significant burden on the global healthcare system due to its rapid transmission and widespread distribution. We have endeavoured to minimise the viral burden in HCV patients in vitro using the NTP, anticipating its influence on serum samples. Meanwhile, the investigation examined oxidative stress in patients with HCV subsequent to NTP exposure through the quantification of reactive oxygen species (ROS), nitric oxide (NO), malondialdehyde (MDA), protein carbonyl (PC), and 8-hydroxydeoxyguanosine (8-OHG) concentrations. Sixty serum samples were collected from patients infected with HCV and subjected to NTP for durations of 60 seconds and 240 seconds. The obtained outcomes were contrasted with those of ninety healthy controls and HCV patients who had not been exposed to radiation. A 240-second exposure to NTP significantly decreased viral load (P0.05), whereas a 60-second exposure did not yield a statistically significant decrease in viral load. Conversely, the concentrations of reactive oxygen species, nitrogen dioxide, pyridoxal-5-oxide, and 8-hydroxygen were significantly increased in patients with HCV when compared to the control group. Oxidative stress was significantly increased by 240 seconds of NTP exposure, as measured by increases in MDA, PC, and NO, but not ROS and 8-OHG. It was demonstrated that nitric oxide is the most dependable indicator for forecasting the prognosis of HCV patients. NTP can ultimately reduce the viral load associated with HCV infections in the laboratory, but it also increases oxidative stress, which may result in systemic oxidative damage. [ABSTRACT FROM AUTHOR]