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Obesity is a metabolic disorder which has become an epidemic worldwide. Bariatric surgery and extended lifestyle adaptation are currently the most effective and long-lasting methods of weight loss. Physical activity (PA) is an essential element of the medical management of patients which subsequent bariatric surgery. Aim of the study: evaluate the effectiveness of physical activity program for bariatric surgery patients. Subjects and method: Quasi-experimental (pre-posttest) research design was used. The study included 70 patients from out-patients surgery clinic Assiut University Hospital. An interview structured questionnaire was used which included personal characteristics, medical history, and an International physical activity questionnaire. The study was carried through 3 stages: preparatory, implementation, and evaluation stage. Results: There was a highly statistically significant difference (P-value=0.000) between the mean score of pre and post-test physical activity program. It showed that 1.4 % of studied patients had high physical activity in the pretest while in posttest 35.7% had high physical activity. Conclusion: Physical activity instructions in the postoperative management of patients appear to increase physical activity and maintain weight loss after bariatric surgery. Recommendations: Further studies are needed to confirm these hypotheses with a larger sample size.

Background: Chest X-ray continues to have an important role in diagnosis of heart diseases.Aim of Study: We aim by this the study to evaluate the use of chest X-ray in the Pediatric Cardiology Unit in Assiut University Children Hospital for children (1-4 years old) with suspected heart disease.Patients and Methods: This was a six-month, case series clinical study undertaken in the inpatient Pediatric Cardiology Unit, Assiut University Hospital, Assiut City, Egypt; between 1 of April 2016 to 30 of September 2016.Results: Chest X-ray is an important tool in evaluating heart disease in children. Noninvasive imaging such as echocar-diography and cardiac MRI provide valuable and detailed assessment of the cardiovascular system; however, the cost incurred from these diagnostic procedures is significant making their routine use difficult.Conclusion: Auditing the guidelines of interpretation of the chest radiograph for children aged one year to four years at the Pediatric Cardiology Unit of Assiut University Hospital for Children, it could be concluded: Chest roentgenogram is accurate in detecting cardiac site and situs.

Granzyme B-expressing (GrB+) B cells are thought to contribute to immune dysfunctions in HIV patients, but so far their exact role is unknown. This report demonstrates for the first time the existence of GrB+ B cells in SIV-infected rhesus macaques, which represent the most commonly used nonhuman primate model for HIV research. Similar to HIV patients, we found significantly higher frequencies of these cells in the blood of chronically SIV-infected rhesus monkeys compared with uninfected healthy ones. These frequencies correlated with plasma viral load and inversely with absolute CD4 T-cell counts. When investigating GrB+ B cells in different compartments, levels were highest in blood, spleen and bone marrow, but considerably lower in lymph nodes and tonsils. Analysis of expression of various surface markers on this particular B-cell subset in SIV-infected macaques revealed differences between the phenotype in macaques and in humans. GrB+ B cells in SIV-infected rhesus macaques exhibit an elevated expression of CD5, CD10, CD25 and CD27, while expression of CD19, CD185 and HLA-DR is reduced. In contrast to human GrB+ B cells, we did not observe a significantly increased expression of CD43 and CD86. B-cell receptor stimulation in combination with IL-21 of purified B cells from healthy animals led to the induction of GrB expression. Furthermore, initial functional analyses indicated a regulatory role on T-cell proliferation. Overall, our data pave the way for longitudinal analyses including studies on the functionality of GrB+ B cells in the nonhuman primate model for AIDS.

Granzyme B-expressing (GrB+) B cells are thought to contribute to immune dysfunctions in HIV patients, but so far their exact role is unknown. This report demonstrates for the first time the existence of GrB+ B cells in SIV-infected rhesus macaques, which represent the most commonly used nonhuman primate model for HIV research. Similar to HIV patients, we found significantly higher frequencies of these cells in the blood of chronically SIV-infected rhesus monkeys compared with uninfected healthy ones. These frequencies correlated with plasma viral load and inversely with absolute CD4 T-cell counts. When investigating GrB+ B cells in different compartments, levels were highest in blood, spleen and bone marrow, but considerably lower in lymph nodes and tonsils. Analysis of expression of various surface markers on this particular B-cell subset in SIV-infected macaques revealed differences between the phenotype in macaques and in humans. GrB+ B cells in SIV-infected rhesus macaques exhibit an elevated expression of CD5, CD10, CD25 and CD27, while expression of CD19, CD185 and HLA-DR is reduced. In contrast to human GrB+ B cells, we did not observe a significantly increased expression of CD43 and CD86. B-cell receptor stimulation in combination with IL-21 of purified B cells from healthy animals led to the induction of GrB expression. Furthermore, initial functional analyses indicated a regulatory role on T-cell proliferation. Overall, our data pave the way for longitudinal analyses including studies on the functionality of GrB+ B cells in the nonhuman primate model for AIDS.