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Objective: To investigate the effects of combining a Pilates program with photobiomodulation therapy (PBMT) in patients with chronic nonspecific low back pain (CNLBP)., Methods: Thirty-eight adults with CNLBP were randomly assigned to two groups: Pilates exercise + active PBMT (PIL + PBMT) or Pilates exercise + sham PBMT (PIL + SHAM). Both groups performed an 8-week mat Pilates program and received PBMT on their lumbar muscles 10 min before and after each session. The following variables were assessed before and after intervention: peak pain intensity, postural balance (i.e., center of the pressure [A-COP], velocity anteroposterior [Vel AP], and velocity mediolateral [Vel ML]), perceived disability (i.e., Oswestry Disability Index [ODI] and Roland Morris Disability Questionnaire [RMDQ]), and pain-related fear of movement (i.e., Tampa Scale of Kinesiophobia [TSK], Fear Avoidance Beliefs Questionnaire [FABQ], and Pain Catastrophizing Scale [PCS])., Results: Postural balance variables showed no statistically significant differences ( p > 0.05) across time or between groups. The groups showed similar ( p < 0.05) reductions in peak pain intensity, ODI, RMDQ, and PCS scores, but no statistically significant difference ( p > 0.05) in TSK and FABQ scores., Conclusion: The mat Pilates program reduced peak pain intensity, perceived disability, and pain catastrophizing in adults with CNLBP, but PBMT had no additional effect on these variables. Mat Pilates alone or combined with PBMT was not able to improve postural balance.

Photobiomodulation therapy (PBMT) has been advocated as a potential intervention to improve muscle performance and recovery in the health and sports context. However, the short- and long-term effects of PBMT on endurance running performance remain under-researched and controversial. The purpose of this study was to investigate the acute dose-response effect of PBMT with light-emitting diodes (LEDs) on endurance performance and rating of perceived exertion (RPE; 6-20 Borg) during a 5-km running trial in recreational runners. In a crossover design, eighteen young adult runners (28.7 ± 7.8 years) were randomized to receive 1 of 4 PBMT conditions (placebo, 300, 900, and 1260 Joules [J]) 60 min before the 5-km running trial on four occasions, separated by a 2-wk washout period. The treatments were applied to the quadriceps, hamstrings, and gastrocnemius muscles of both legs using a device containing 200 LEDs (100 red and 100 infrared). The following variables were assessed: endurance performance (i.e. total time, mean velocity, and velocity in the split distances at the initial 200 m and every 400 m lap) and RPE in the split distances at the initial 200 m and every 400 m lap. Data normality and homogeneity were tested using Shapiro-Wilk's and Levene's tests, respectively. Differences between treatment conditions were assessed using the analysis of variance tests (one- or two-way ANOVA, depending on the comparisons), complemented by the Bonferroni post hoc test. There were significant time effects for the running velocity and RPE in the split distances (p < 0.0001), with no significant treatment-by-time interaction (running velocity, p = 0.59; RPE, p = 0.95). The mean velocity (p = 0.997), total time (p = 0.998), and total mean of the RPE (p = 0.91) were similar between treatment conditions. In conclusion, acute PBMT with LEDs at doses of 300, 900, and 1260 J is not recommended for improving endurance performance and RPE in the 5-km running trial in recreational runners., (© 2024. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)

Alzheimer's disease (AD) represents the most common type of dementia. A crucial mechanism attributed to its development is amyloid beta (Aβ) dynamics dysregulation. The extent to which exercise can modulate this phenomenon is uncertain. The aim of this study was to summarize the existing literature evaluating this issue. A comprehensive systematic search was performed in Pubmed, Scopus, Embase, Web of Science, and SciELO databases and completed in August 2023, aiming to identify randomized controlled trials investigating the effect of exercise upon Aβ-related pathology. The keywords "exercise" and "amyloid beta", as well as all their equivalents and similar terms, were used. For the analysis, the negative or positive dementia status of the subjects was initially considered and then the soluble amyloid precursor protein (sAPP) components and Aβ fragments separately. A meta-analysis was performed and involved eight studies (moderate-to-high quality) and 644 assessments, which were 297 for control and 347 for exercise. No overall effect favoring exercise interventions was observed for both negative (SMD95%=0,286 [-0,131; 0,704]; p = 0,179) or positive AD dementia status (SMD95%=0,110 [-0,155; 0,375]; p = 0,416). The absence of an overall effect favoring exercise interventions was also found for Aβ peptides (SMD95%=0,226 [-0,028; 0,480]; p = 0,081) and for sAPP components (SMD95%=-0,038 50 [-0,472; 0,396]; p = 0,863) levels. Our findings suggest that exercise interventions do not improve Aβ-related pathology in both healthy individuals and individuals with dementia (SMD95%=0,157 [-0,059; 0,373]; p = 0,155), indicating that the beneficial effects of exercise for AD reported in previous studies are related to other mechanistic effects rather than direct amyloid effects (PROSPERO registration number: CRD42023426912)., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Objective: To conduct a meta-analysis to investigate the effects of Photobiomodulation (PBM) therapy on running performance., Introduction: PBM has recently been advocated as a valuable non-pharmacological ergogenic strategy, however, the efficacy of PBM on running performance remains unproven., Methods: A computerized literature search was conducted until June 2023. The databases searched were PubMed/Medline, Embase, Scopus, SPORTDiscus, and Web of Science. Inclusion/exclusion criteria were determined through the PICO process. The running variables analyzed were time-trial or time-to-exhaustion. Results were combined with the standardized mean differences (SMD) and the 95% confidence intervals., Results: Twelve studies fulfilled the inclusion criteria. No significant effects in favor of PBM were found (SMD = 0.13; p = 0.11). There was no effect considering the presence (SMD = 0.16; p = 0.38) and absence (SMD = 0.11; p = 0.25) of training, and there was no dose-response effect ( p = 0.82)., Conclusion: Our findings indicate that PBM alone or combined with a training program does not improve running performance in terms of time-trial and time-to-exhaustion testing. More studies involving PBM plus training and doses higher than 1000 J are needed to determine if PBM is effective in improving running performance.

Patients experiencing a cerebrovascular event are vulnerable to declining nutritional status, hindering rehabilitation. This study aims to analyze the association between malnutrition risk and hospital rehabilitation indicators in ischemic stroke survivors (ISS). This analytical study examined medical records of 160 adult patients (69.3 ± 13 years). Undernutrition risk (UR; independent variable) and rehabilitation indicators (dependent variables) like hospital stay, clinical outcome, functionality, stroke severity, food intake, mobility (bedridden), mechanical ventilation, and enteral nutrition were assessed. Data were dichotomized, and the chi-square test identified associations (p ≤ 0.05), followed by Poisson regression for prevalence ratios. Patients at UR had 2-fold higher risk of death (95% confidence interval [CI], 0.99-4.79), 1.8-fold higher risk of high stroke severity (95% CI, 1.06-3.11), 76% higher chance of being bedridden (95% CI, 1.28-2.44), and 3-fold higher risk of mechanical ventilation (95% CI, 1.20-9.52). UR in hospitalized ISS is associated with deteriorating rehabilitation indicators, including mobility, decreased food intake, mechanical ventilation use, and neurological deficit, indicating an increased mortality risk post-stroke., Competing Interests: Conflict of Interest: The authors have no potential conflicts of interest to disclose., (Copyright © 2024. Korean Society for Neurorehabilitation.)

Background: The Xpert® MTB/RIF rapid molecular test provides a quantitative measure of Mycobacterium tuberculosis (Mtb) DNA in the form of cycle threshold (Ct) values. This information can be translated into mycobacterial load and used as a potential risk measure of bacterial spread for tuberculosis cases, which can impact infection control. However, the role of Ct values in assessing Mtb transmission to close contacts has not yet been demonstrated., Methods: A prospective study was performed to investigate the association between Xpert® MTB/RIF Ct values and Mtb transmission to close contacts of patients with culture-confirmed pulmonary TB in a multi-center Brazilian cohort. We evaluated clinical and laboratory data, such as age, sex, race, smoking habits, drug use, alcohol use, chest radiograph, Xpert® MTB/RIF results among pulmonary tuberculosis cases, and QuantiFERON(QFT)-Plus results at baseline and after six months for close contacts who had a negative result at baseline., Results: A total of 1,055 close contacts of 382 pulmonary tuberculosis cases were included in the study. The median Ct values from pulmonary tuberculosis cases of QFT-Plus positive (at baseline or six months) close contacts were lower compared with those who were QFT-Plus negative. An adjusted logistic regression demonstrated that reduced Ct values from the index cases were independently associated with QFT-Plus conversion from negative to positive (OR: 1.61, 95% CI: 1.12-2.32) after adjusting for clinical characteristics., Conclusion: Close contacts of pulmonary TB index cases exhibiting low Xpert MTB/RIF Ct values displayed higher rates of TB infection, reflecting Mtb transmission., (© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Background: Citrulline malate (CM), especially when used in conjunction with physical exercise, has demonstrated potential as a non-pharmacological adjunct in the management of hypertension. Nevertheless, its impact on nocturnal blood pressure dipping remains unexplored., Objective: Evaluate the impact of a single dose of CM on nocturnal blood pressure dipping after exercise in hypertensive individuals., Methods: In a double-blind, placebo-controlled, parallel-group clinical trial, twenty hypertensive adults (55 ± 16 years) were randomly assigned to either a CM (6 g) or placebo (6 g of corn starch) group (PLA). Resting blood pressure was measured after a 20-min period of comfortable seating in a calm environment. Both groups underwent 40 min of treadmill running/walking at an intensity of 60-70% of their reserve heart rate, 120 min after ingesting the substances. Ambulatory blood pressure monitoring was employed to measure blood pressure over 24 h., Results: No significant differences in systolic blood pressure values were observed between the CM and PLA groups at rest, during wakefulness, sleep, or over a 24-h period. However, CM exhibited a significant reduction in diastolic blood pressure in several metrics: delta 24 h (-14 mmHg vs -6 mmHg, p  = 0.047), delta wakefulness (-12 mmHg vs -4 mmHg, p  = 0.024), percent delta 24 h (-16% vs -6%, p  = 0.024), and percent delta wakefulness (-14% vs -4%, p  = 0.013). No significant differences were found between CM and PLA in terms of systolic and diastolic nocturnal absolute reductions (-13 mmHg vs -12 mmHg, p  = 0.808, and -13 mmHg vs -8 mmHg, p  = 0.273, respectively) or nocturnal percentage decrease (-9.9% vs -9.4%, p  = 0.844, and -15.3% vs -11.7%, p  = 0.399, respectively)., Conclusions: The study found no significant changes in the post-exercise nocturnal blood pressure dip following a single dose of CM supplementation. However, a notable reduction in diastolic blood pressure was observed during the waking period and over the average 24-h monitoring period., Clinical Trial Registry Number and Website: ClinicalTrials.gov platform (NCT03378596).

Purpose: Delayed-onset muscle soreness (DOMS) describes an entity characterized by ultrastructural muscle damage. Hesperidin methyl chalcone (HMC) is a synthetic flavonoid presenting analgesic, anti-inflammatory, and antioxidant properties. We evaluated the effects of HMC upon DOMS., Method: In a preventive paradigm, 31 sedentary young men were submitted to a randomized, double-blinded parallel trial and received HMC 500 mg or one placebo capsule × 3 days before an intense dynamic exercise protocol (concentric/eccentric actions) applied for lower limbs for inducing muscle damage. Assessments were conducted at baseline, and 24 and 48 h after, comprising physical performance, and post-muscle soreness and damage, inflammation, recovery of muscle strength, and postural balance associated with DOMS. HMC safety was also evaluated. Thirty participants completed the study., Results: HMC improved the performance of participants during exercise (40.3 vs 51.3 repetitions to failure, p = 0.0187) and inhibited CPK levels (90.5 vs 57.9 U/L, p = 0.0391) and muscle soreness during passive quadriceps palpation (2.6 vs 1.4 VAS cm, p = 0.0439), but not during active actions, nor did it inhibit IL-1β or IL-10 levels. HMC improved muscle strength recovery, and satisfactorily refined postural balance, without inducing injury to kidneys or liver., Conclusions: Preemptive HMC supplementation may be beneficial for boosting physical performance and for the amelioration of clinical parameters related to DOMS, including pain on muscle palpation, increased blood CPK levels, and muscle strength and proprioceptive deficits, without causing adverse effects. These data advance the understanding of the benefits provided by HMC for DOMS treatment, which supports its usefulness for such purpose., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

This study's purpose was to compare the effects of different carbohydrate (CHO) intakes on body composition and muscular strength following eight weeks of resistance training (RT) in pre-conditioned men. In addition, we explored the individual responses to different CHO intakes. Twenty-nine young men volunteered to participate in this study. The participants were divided into two groups according to their relative CHO intake: lower (L-CHO; n = 14) and higher (H-CHO; n = 15). Participants performed a RT program four days a week for eight weeks. The lean soft tissue (LST) and fat mass were determined by dual-energy X-ray absorptiometry. Muscular strength was determined by a one-repetition maximum (1RM) test in the bench press, squat, and arm curl exercises. Both groups increased LST ( P < 0.05) with no statistical differences between conditions (L-CHO = +0.8% vs. H-CHO = +3.5%). Neither group demonstrated changes in fat mass. Both groups increased 1RM ( P < 0.05) in the bench press (L-CHO = +3.6% vs. H-CHO = +5.8%) and squat (L-CHO = +7.5% vs. H-CHO = +9.4%); however, only H-CHO significantly increased arm curl 1RM ( P < 0.05) at post-training (L-CHO = +3.0% vs. H-CHO = +6.6%). Responsiveness was greater in H-CHO vs. L-CHO for LST and arm curl 1RM. In conclusion, lower and higher CHO intakes promote similar increase in LST and muscular strength; however, a greater intake may improve the responsiveness to gains in lean mass and arm curl strength in pre-conditioned men.

Aim: To evaluate the influence of high-intensity interval training (HIIT) on cardiac structural and functional characteristics and myocardial mitogen-activated protein kinase (MAPK) signaling in hypertensive rats., Methods: Male rats (12 months old) were divided into three groups: Wistar Kyoto rats (WKY, n = 8); sedentary spontaneously hypertensive rats (SED-SHR, n = 10), and trained spontaneously hypertensive rats (HIIT-SHR, n = 10). Systolic blood pressure (SBP), functional capacity, echocardiography, isolated papillary muscle, and gene expression of MAPK gene-encoding proteins associated with Elk1, cJun, ATF2, MEF2 were analyzed., Key Findings: HIIT decreased SBP and increased functional capacity, left ventricular diastolic diameter, posterior wall thickness-left ventricle, relative wall thickness-left ventricle, and resting tension of the papillary muscle. In hypertensive rats, we observed a decrease in the gene-encoding ATF2 protein; this decrease was reversed by HIIT., Significance: The influence of HIIT in the SHR model in the compensated hypertension phase generated an increase in cardiac hypertrophy, attenuated myocardial diastolic dysfunction, lowered blood pressure, improved functional capacity, and reversed the alteration in gene-encoding ATF2 protein., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Abstract: Nunes, JP, Marcori, AJ, Ribeiro, AS, Cunha, PM, Kassiano, W, Costa, BDV, Aguiar, AF, Nakamura, M, Mayhew, JL, and Cyrino, ES. Differential responsiveness for strength gain between limbs after resistance training in older women: Impact on interlimb asymmetry reduction. J Strength Cond Res 36(11): 3209-3216, 2022-The present study compared strength responses between preferred (PREF) and nonpreferred (N-PREF) legs in older women. Muscular strength was measured unilaterally using an isokinetic dynamometer and was analyzed for reproducibility scores, acute performance, and responsiveness to a resistance training (RT) program. One hundred eleven women (aged ≥60 years) performed 12 weeks of whole-body RT (3 times a week; 4 lower-body exercises). Reproducibility scores (intraclass correlation coefficient ≥0.920; following test-retests in part of the sample at pretraining; n = 19), average acute performances, and average strength gains (PREF = ∼6.9%; N-PREF = ∼7.2%) were similar between legs (p > 0.05). However, the individual analyses showed that some subjects were considered responders to strength gains in 1 leg, whereas nonresponders in the other. Nonetheless, when considering the responses in all strength tests, most subjects (91%) were considered responder to at least 1 measure. In addition, it was observed that the strength ratio between PREF/N-PREF legs was altered for those who presented some asymmetry at baseline, as the limbs became more symmetrical in all strength measures after the RT. In conclusion, we observed that some older women may have different levels of strength between legs; however, a traditional 12-week RT program with bilateral exercises can reduce such asymmetry by inducing greater strength gains in the weaker leg., (Copyright © 2022 National Strength and Conditioning Association.)

Background and Purpose: Physical and therapeutic strategies to maintain and rehabilitate skeletal muscle mass, strength, and postural balance are clinically relevant to improve the health, well-being, and quality of life of older adults. The purpose of this study was to investigate the effects of photobiomodulation (PBM)/laser therapy combined with a resistance training (RT) program on quadriceps hypertrophy and strength, and postural balance in older women., Methods: In a randomized, triple-blinded, placebo-controlled design, twenty-two older women (age 66.6 ± 5.2 years) were engaged in a supervised 10-wk RT program (2 times per week) involving unilateral leg extension exercise, in which each leg of the same participant was randomly assigned to receive active (λ = 808 nm, optical output = 100 mW, total energy = 42 J) or placebo laser PBM immediately before the RT sessions. Maximal dynamic strength by unilateral knee extension 1-repetition maximum (1RM), muscle hypertrophy by vastus lateralis muscle thickness, and postural balance by one-legged stance test on a force platform were assessed before and after the training program., Results: Significance statistical analysis revealed a similar improvement (time P = .003) from pre- to posttraining for muscle hypertrophy and strength, and postural balance between active and placebo laser conditions. However, clinical interpretation for muscle hypertrophy showed a moderate effect (effect size [ES] = 0.58) for the active laser and a small effect (ES = 0.38) for the placebo laser. Clinical difference was not noticed between conditions for other analyzed variables., Conclusions: These findings indicate that RT alone can be clinically important for counteracting the deleterious effects of aging on muscle size, strength, and balance, and that applying laser PBM therapy before the RT sessions may further improve gains in muscle hypertrophy., Competing Interests: The authors declare no conflicts of interest., (Copyright © 2021 APTA Geriatrics, An Academy of the American Physical Therapy Association.)

Aims: The main purpose of the present study was to investigate the acute effects of different resistance training loads on the session rating of perceived exertion (sRPE) and feelings of pleasure displeasure (sFPD) of the session in older women., Methods: In a randomized, counterbalanced, and cross-over design, 16 older women (66.1 ± 3.9 years, 67.0 ± 8.4 kg, 154.1 ± 6.8 cm, 28.3 ± 4.4 kg/m 2 ) performed 3 sets of 8 exercises in three experimental conditions, 1 session with a heavy load (HEV), one with a moderate load (MOD), and one session with a light load (LIT), all performed until momentary muscle failure. The sRPE and sFPD were assessed 15 min after the end of each session using specific scales., Results: A significant difference (P < 0.05) was observed among conditions for sFPD in which HEV produced feelings of displeasure, and MOD produced greater feelings of pleasure (HEV = -1.38 ± 1.7, MOD = 3.38 ± 1.2, LIT = 0.69 ± 1.04). For sRPE, a significant (P < 0.05) difference was observed between MOD and LIT; whereby the LIT load induced a greater score compared to the MOD load (HEV = 4.56 ± 1.4, MOD = 4.31 ± 1.7, LIT = 5.75 ± 1.2)., Conclusion: Our results suggest that the training load affects perceived effort and feelings of pleasure/displeasure when exercise is performed until concentric failure. The use of moderate loads induces greater pleasure feelings and a reduced degree of effort in older women., (© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Photobiomodulation therapy (PBM) is often used to treat musculoskeletal disorders such as chronic non-specific low back pain (NSCLBP) as it can have positive effects on biomarkers-creatine kinase (CK) and serum cortisol levels-related to stress caused by physical exercise, such as deep water running (DWR) or by pain. The aim of this study was to evaluate the effects of the combination of PBM and aquatic exercise (DWR) on the intensity of pain, disability, 6-min walk test adapted (6WTA), and on cortisol and creatine kinase (CK) levels in a population with NSCLBP. The participants were allocated into three groups: TG PBM (Photobiomodulation and Training Group), TG PLA (Placebo Photobiomodulation and Training Group), and the G PBM (Photobiomodulation Group). Information regarding anthropometric data, blood pressure, and heart rate were collected, and the questionnaires were applied: IPAQ-Short Form, Oswestry Disability Index, and the Visual Analog Scale for Pain. The submaximal exercise test (6WTA) was performed. Blood was collected for analysis of cortisol and CK levels. The training sessions were performed twice a week, for 4 weeks. In the intragroup comparisons, there were statistically significant changes in the TG PBM and G PBM groups in the outcomes pain intensity, disability (reductions in both groups), and in cortisol (increased in the TG PBM and reduced in the G PBM ); in the TG PLA group, there was a statistically significant reduction only in the outcome of pain intensity. In the intergroup comparison, in the comparison between TG PBM and TG PLA , there was a statistically significant difference in the level of cortisol, as well as in the comparison between TG PBM and G PBM , in which there was a statistically significant difference for this same outcome (cortisol) and for the 6WTA outcome. The effects of the combination of PBM and aquatic exercise have positive effects on reducing pain intensity, disability, and cortisol levels, but its effects on other variables (6WTA and CK) are too small to be considered significant. Trial registration number: NCT03465228-April 3, 2019; retrospectively registered (ClinicalTrials.gov)., (© 2022. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)

Purpose: Resistance training (RT) combined with appropriate dietary intake can promote a concomitant increase in skeletal muscle mass (SMM) and reduction in fat mass, a condition termed body recomposition. This study's primary purpose was to explore the effects of protein ingestion on body recomposition after 24 wk of RT in older women., Methods: Data from 130 untrained older women (68.7 ± 5.6 yr, 66.5 ± 11.5 kg, 155.5 ± 6.0 cm, and 27.4 ± 4.0 kg·m-2) across six studies were retrospectively analyzed. The participants were divided into tertiles according to their customary protein intake (g·kg-1·d-1): lower (LP; n = 45), moderate (MP; n = 42), and higher (HP; n = 43) protein intake. Participants performed a whole-body RT program carried out over 24 wk (eight exercises, three sets, 8-15 repetitions, three sessions a week). SMM and fat mass were determined by dual-energy x-ray absorptiometry., Results: All groups increased SMM from baseline (P < 0.05), with the HP and MP groups showing greater increases than the LP group (LP, 2.3%; MP, 5.4%; and HP, 5.1%; P < 0.05). Reductions in fat mass were similar for all three groups (LP, 1.7%; MP, 3.7%; and HP, 3.1%; P > 0.05). The composite z-score of the percentage changes from pretraining to posttraining indicated greater positive body recomposition values for HP and MP compared with LP (P < 0.05)., Conclusions: Results suggest that protein intake is a moderating variable for body recomposition in older women undergoing RT, with a low protein intake having a less favorable effect on body recomposition., (Copyright © 2022 by the American College of Sports Medicine.)

The purpose of this study was to investigate the effects of supplementation of whey protein (WP) versus leucine-matched collagen peptides (CP) on muscle thickness MT and performance after a resistance training (RT) program in young adults. Twenty-two healthy untrained participants were randomly assigned to either a WP (n = 11) or leucine-matched CP (n = 11) group and then submitted to a supervised 10-week RT program (3 days/week). The groups were supplemented with an equivalent amount of WP (35 g, containing 3.0 g of leucine) and CP (35 g, containing 1.0 g of leucine and 2.0 g of free leucine) during the intervention period (after each workout and in the evening on nontraining days). MT of the vastus lateralis and biceps brachii, isokinetic peak torque and mean power output of the elbow flexors, and peak power output of the lower body were assessed before and after the RT program. The WP group experienced a greater (interaction, p < .05) increase in the vastus lateralis (effect size, WP = 0.68 vs. CP = 0.38; % Δ, WP = 8.4 ± 2.5 vs. CP = 5.6 ± 2.6%) and biceps brachii muscle thickness (effect size, WP = 0.61 vs. CP = 0.35; % , WP = 10.1 ± 3.8 vs. CP = 6.0 ± 3.2%), with a similar increase in muscle performance (peak torque, mean power output, and peak power output) between groups (time p < .05). Supplementation with WP was superior to leucine content-matched CP supplementation in increasing muscle size, but not strength and power, after a 10-week RT program in young adults.

Background: Syrphid flies are important ecological indicators and provide crucial ecosystem services, being important pollinators and biological control agents of insect pests. These charismatic insects are conspicuous and, due to their size and colourful patterns, are relatively easy to identify. However, the lack of user-friendly literature (e.g. photographic guides) for most areas may hamper its wider selection as a study group in biodiversity and ecological studies. The syrphid fauna of Madeira Archipelago comprises 26 species, including four endemics ( Eumerushispidus Smit, Aguiar & Wakeham-Dawson, 2004; Melanostomawollastoni Wakeham-Dawson, Aguiar, Smit, McCullough & Wyatt, 2004; Myathropausta , Wollaston, 1858 and Xanthandrusbabyssa , Walker, 1849), but, despite the current good taxonomic knowledge on this group, information on species distribution, ecology and conservation is still lacking. Here, we provide a pictorial key to the adult hoverflies of Madeira Archipelago highlighting diagnostic characteristics and present photographs of both males and females (in dorsal and lateral views) in colour plates. The key and plates will help researchers to differentiate these species, thus encouraging the use of this insect group in future bioindication studies. In addition, this study also aims to engage a broader audience of non-experts in improving the knowledge on the distribution and ecology of Madeira syrphids., New Information: We provide a checklist for the hoverflies of Madeira Archipelago and a pictorial key to help on species identification., (Carla Rego, John Smit, António Franquinho Aguiar, Délia Cravo, Andreia Penado, Mário Boieiro.)

Although the ergogenic mechanisms of supplementation with citrulline malate are well known, unclear findings regarding variables of muscle strength have been recorded. Such misleading findings in the literature illustrate the need for well-conducted meta-analysis research to elucidate the possible ergogenic impact, which could have major practical consequences for athletes and recreational practitioners seeking to optimize gains in muscle strength. The objective of this systematic review was to summarize the existing literature that evaluated the effects of citrulline malate supplementation on muscle strength outcomes from resistance exercise in resistance-trained individuals. A systematic electronic search in Medline and Scientific Electronic Library Online (SciELO) was completed in August 2020 identifying randomized controlled trials investigating the effect of citrulline malate supplementation on muscle strength in resistance-trained adults. A subsequent meta-analysis was performed. The meta-analysis involved four studies and 138 assessments (69 in citrulline-malate and 69 in placebo groups). We did not observe an overall effect favoring citrulline-malate supplementation (SMD 95% = 0.13 [-0.21; 0.46]). Considering the lower (SMD 95% = 0.06 [-0.47; 0.60]) and upper (SMD 95% = 0.17 [-0.26; 0.60]) limbs, a non-significant overall effect was identified. The mean effects were similar for "limbs" (upper vs lower) [ p  = 0.763]. Accordingly, our findings suggest that citrulline malate supplementation does not improve muscle strength in healthy and resistance-trained individuals (PROSPERO registration number: CRD42020159338).

To gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in four groups: Sedentary Control (S, n = 8); Control with Training (T, n = 8); Sedentary with Pulmonary Arterial Hypertension (SPAH, n = 8); and Pulmonary Arterial Hypertension with Training (TPAH, n = 8). All rats underwent a two-week adaptation period; T and TPAH group rats then proceeded to an eight-week training period on a treadmill. At the beginning of the 11th week, S and T groups received an intraperitoneal injection of saline, and SPAH and TPAH groups received an injection of monocrotaline (60 mg/kg). Rats in the T and TPAH groups then continued with the training protocol until the 13th week. We assessed exercise capacity, echocardiography analysis, Fulton's index, cross-sectional areas of cardiomyocytes, collagen content and types, and fractal dimension (FD). Transcript abundance of myosins and extracellular matrix genes were estimated through reverse transcription-quantitative PCR (RT-qPCR). When compared to the SPAH group, the TPAH group showed increases in functional capacity and pulmonary artery acceleration time/pulmonary ejection time ratio and decreases in Fulton's index and cross-sectional areas of myocyte cells. However, preventive exercise did not induce alterations in col1a1 and myh7 gene expression. Our findings demonstrate that preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated PH development without interfering in mRNA-encoding myosin and collagen expression during PAH., Competing Interests: The authors have declared that no competing interests exist.

A comprehensive annotated checklist of the ladybeetle species of Portugal, including the Azores and Madeira archipelagos, is presented. The Coccinellidae fauna comprises a total of 101 species: 83 from the Mainland, 39 from Madeira, and 32 from the Azores. The listed species are distributed among 2 sub-families and 13 tribes: within the subfamily Microweiseinae, Madeirodulini (1 species), Serangiini (2 species), and within the subfamily Coccinellinae, Azyini (1 species), Chilocorini (4 species), Coccidulini (7 species), Coccinellini (30 species), Epilachnini (4 species), Hyperaspidini (7 species), Noviini (2 species), Platynaspini (1 species), Scymnini (37 species), Stethorini (3 species), and Sticholotidini (2 species). The Portuguese fauna comprises 10 exotic species: 5 present in the Mainland, 7 in Madeira, and 6 in the Azores. Harmoniaaxyridis (Pallas, 1773) from Madeira, Propyleaquatuordecimpunctata (Linnaeus, 1758) from the Azores, Delphastuscatalinae (Horn, 1895) from the Azores and Madeira, Nephus (Geminosipho) reunioni (Fürsch, 1974) and Nephus (Nephus) voeltzkowi Weise, 1910 from Madeira and Microserangium sp. from the Mainland, are reported for the first time. Some species are considered doubtful records, as explained in the text. These results were obtained by compiling information on the available literature regarding ladybeetle species on the Portuguese mainland and insular territories, and original data., (António Onofre Soares, Hugo Renato Calado, José Carlos Franco, António Franquinho Aguiar, Miguel M. Andrade, Vera Zina, Olga M.C.C. Ameixa, Isabel Borges, Alexandra Magro.)

Objective: This study aims to investigate the effects of laser photobiomodulation (PBM) at 808 nm on biceps brachii performance to exhaustion, rating of perceived exertion (RPE), and delayed onset muscle soreness (DOMS) in untrained young women., Methods: Thirteen young women (20.1 ± 2.9 years) participated in a crossover study in which they received, in a counterbalanced manner, active and placebo laser PBM on two occasions (T1 and T2), separated by a 7-day washout period. During T1 and T2, participants received active (100 mW output power, irradiance of 35.7 W cm -2 , and total energy of 28 J/arm) or placebo laser irradiation on the biceps brachii muscle at 20 min before the repetitions-to-failure test [six sets at 60% of one-repetition maximum (1RM) until failure] for elbow flexion exercise. The number of repetitions performed and RPE over the six sets, as well as DOMS from basal up to 72 h after the repetitions-to-failure test, were recorded., Results: There was a significant (time, p < 0.05) reduction in the number of repetitions performed and an increase in RPE over six sets, with no statistical differences between placebo and active laser conditions (treatment × time, p > 0.05). DOMS increased at 24 h postexercise and progressively returned to baseline after 72 h in both conditions (time, p < 0.05; treatment × time, p > 0.05)., Conclusion: Our results indicate that acute laser PBM at 808 nm does not improve biceps brachii performance to exhaustion, RPE, and DOMS in untrained women., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Azuma, Merlo, Jacinto, Borim, da Silva, Pacagnelli, Nunes, Ribeiro and Aguiar.)

Specialized resistance training techniques (e.g., drop-set, rest-pause) are commonly used by well-trained subjects for maximizing muscle hypertrophy. Most of these techniques were designed to allow a greater training volume (i.e., total repetitions×load), due to the supposition that it elicits greater muscle mass gains. However, many studies that compared the traditional resistance training configuration with specialized techniques seek to equalize the volume between groups, making it difficult to determine the inherent hypertrophic potential of these advanced strategies, as well as, this equalization restricts part of the practical extrapolation on these findings. In this scenario, the objectives of this manuscript were 1) to present the nuance of the evidence that deals with the effectiveness of these specialized resistance training techniques and - primarily - to 2) propose possible ways to explore the hypertrophic potential of such strategies with greater ecological validity without losing the methodological rigor of controlling possible intervening variables; and thus, contributing to increasing the applicability of the findings and improving the effectiveness of hypertrophy-oriented resistance training programs., Competing Interests: The authors certify that there is no conflict of interest relevant to the content of this manuscript., (Thieme. All rights reserved.)

Digoxin is a cardiotonic that increases the cardiac output without causing deleterious effects on heart, as well as improves the left ventricular performance during physical exercise. We tested whether the association between chronic digoxin administration and aerobic interval training (AIT) promotes beneficial cardiovascular adaptations by improving the myocardial contractility and calcium (Ca 2+) handling. Male Wistar rats were randomly assigned to sedentary control (C), interval training (T), sedentary digoxin (DIGO) and T associated to digoxin (TDIGO). AIT was performed on a treadmill (1h/day, 5 days/week) for 60 days, consisting of successive 8-min periods at 80% and 20% of VO 2 máx for 2 min. Digoxin was administered by orogastric gavage for 60 days. Left ventricle samples were collected to analysis of Ca 2+ handling proteins; contractility and Ca 2+ handling were performed on isolated cardiomyocytes. TDIGO group had a greater elevation in fractional shortening (44%) than DIGO, suggesting a cardiomyocyte contractile improvement. In addition, T or TDIGO groups showed no change in cardiomyocytes properties after Fura2-acetoxymethyl ester, as well as in sarcoplasmic reticulum Ca 2+- ATPase (SERCA2a), phospholamban and calcineurin expressions. The main findings indicate that association of digoxin and aerobic interval training improved the cardiomyocyte contractile function, but these effects seem to be unrelated to Ca 2+ handling., Competing Interests: The authors declare no conflicts of interest., (Thieme. All rights reserved.)

This study aimed to compare the effects of resistance training performed with low versus moderate loads on systemic resting blood pressure (BP) in older women. A total of 29 women (72.6 ± 5.1 years) were randomized into two groups: low load (LOW, n = 15) and moderate load (MOD, n = 14). An 8-week whole-body resistance training program was carried out 3 days/week (eight exercises, three sets, 10 or 15 repetition maximum). The LOW and MOD groups trained with a relative load of 15 and 10 repetition maximum, respectively. Outcome measures included resting systolic and diastolic BP. After 8 weeks, both groups presented significant changes (p < .05) in systolic BP (LOW = -3.0%; MOD = -4.6%) and mean BP (LOW = -1.9%; MOD = -3.1%). There was no change for diastolic BP in the posttest in both groups. The results suggest that low and moderate loads are equally effective for promoting decreases in resting BP in older women.

This study aimed to investigate the effects of free leucine supplementation on muscle recovery from resistance exercise (RE) in young adults. Fourteen untrained subjects (23.9 ± 3.6 years old) underwent RE sessions (leg press and hack squat: three sets of 8-12 reps at 70% 1RM) supplemented with leucine (LEU: two daily doses of 3g) or a placebo (PLA), separated by a seven-day washout period. Following each occasion, participants were evaluated in three subsequent days (24h, 48h, and 72h) for muscle recovery via a repetition-to-failure test. The following markers were assessed: repetition performance, perceived exertion, lactate, creatine kinase, muscle soreness (DOMS), testosterone, and cortisol. No significant difference was observed between LEU and PLA conditions ( p > 0.05). Number of repetitions performed in the repetition-to-failure tests, perceived exertion, cortisol, and testosterone:cortisol ratio did not change over time ( p > 0.05). Creatine kinase increased immediately after exercise, at 24h, and 48h, and was attenuated at 72h post-exercise, while testosterone, lactate, and DOMS increased at 24h post-exercise ( p < 0.05) and remained elevated up to 72h. All outcomes were similar between LEU and PLA. Results indicate that a 6g daily dose of free leucine supplementation does not improve muscle recovery following lower-limb RE in untrained young adults.

Context: Chronic low-back pain (CLBP) may be associated with changes in postural balance in athletes as poor postural control during sports practice., Objective: To compare the postural control of athletes with and without CLBP during 2 one-legged stance tasks and identify the center of pressure (COP) cutoff values to determine the main differences. Designed: A cross-sectional study., Setting: Laboratory of functional evaluation and human motor performance., Participants: A total of 56 male athletes, 28 with and 28 without CLBP (mean age = 26 y)., Intervention: The one-legged stance with knee extension and with the knee at 30° flexion tasks were measured and analyzed on a force platform. The participants completed three 30-second trials (30 s of rest between each trial)., Main Outcome Measures: The COP parameters: the area of COP, mean COP sway velocity in both the anteroposterior and mediolateral directions, and total COP displacement were computed, and a receiver operating characteristics curve analysis was applied to determine the group differences., Results: Athletes with CLBP had poorer postural control (P < .01) in both tasks. The 30° knee flexion reported more postural instability than the knee extension for all COP parameters (a large effect size d = 0.80).The knee extension cutoffs identified were >7.1 cm2 for the COP area, >2.6 cm/s for the COP sway velocity in the anterior-posterior direction, and >3.2 cm/s for the mediolateral direction. Whereas, the 30° knee flexion cutoffs were >10.9 cm2 for the COP area, >2.9 cm/s for the COP sway velocity in the anterior-posterior direction, and >4.1 cm/s for the mediolateral direction. Both measures showed enough sensitivity and specificity (ie, area under the curve = 0.88 in and 0.80, respectively) to discriminate both groups., Conclusions: The athletes with CLBP had poorer postural control than the healthy athletes and obtained specific cutoff scores from the COP values.

The authors aimed to compare the effects of creatine (Cr) supplementation combined with resistance training on skeletal muscle mass (SMM), total body water, intracellular water (ICW), and extracellular water (ECW) in resistance-trained men as well as to determine whether the SMM/ICW ratio changes in response to the use of this ergogenic aid. Twenty-seven resistance-trained men received either Cr (n = 14) or placebo (n = 13) over 8 weeks. During the same period, subjects performed two split resistance training routines four times per week. SMM was estimated from appendicular lean soft tissue assessed by dual-energy X-ray absorptiometry. Total body water, ICW, and ECW were determined by spectral bioelectrical impedance. Both groups showed improvements (p < .05) in SMM, total body water, and ICW, with greater values observed for the Cr group compared with placebo. ECW increased similarly in both groups (p < .05). The SMM/ICW ratio did not change in either group (p > .05), whereas the SMM/ECW ratio decreased only in the Cr group (p < .05). A positive correlation was observed (p < .05) between SMM and ICW changes (r = .71). The authors' results suggest that the increase in muscle mass induced by Cr combined with resistance training occurs without alteration of the ratio of ICW to SMM in resistance-trained men.

Muscular strength and hypertrophy following resistance training may be obtained in different degrees depending on the approach performed. This study was designed to compare the responses of the biceps brachii to two preacher curl exercises, one performed on a cable-pulley system (CAB; in which a greater torque was applied during the exercise when elbows were flexed and biceps shortened) and one performed with a barbell (BAR; in which greater torque was applied when the elbows were extended and biceps stretched). Thirty-five young adults (CAB: 13 men, 5 women; BAR: 12 men, 5 women; age = 24 ± 5 years) performed a resistance training program three times per week for 10 weeks, with preacher curl exercises performed in three sets of 8-12 repetitions. Outcomes measured included elbow flexion peak isokinetic torque at angles of 20°, 60°, and 100° (considering 0° as elbow extended), and biceps brachii thickness (B-mode ultrasound). Following the training period, there were significant increases for both groups in elbow flexion peak torque at the 20° (CAB: 30%; BAR = 39%; p = 0.046), 60° (CAB: 27%; BAR = 32%; p = 0.874), and 100° (CAB: 17%; BAR = 19%; p = 0.728), and biceps brachii thickness (CAB: 7%; BAR = 8%; p = 0.346). In conclusion, gains in muscular strength were greater for BAR only at longer muscle length, whereas hypertrophy was similar regardless of whether torque emphasis was carried out in the final (CAB) or initial (BAR) degrees of the range of motion of the preacher curl in young adults.

A checklist of the tephritid fauna (Diptera: Tephritidae) from Madeira archipelago is presented, including 17 known species and new distribution records for Porto Santo and Desertas Islands. An identification key to Madeira Tephritidae species with detailed illustrations is also provided. A new species of Oedosphenella Frey 1936, discovered in Madeira Island in 2004, is described as Oedosphenella bob sp. n. This genus is redefined and a key to the known species is provided. Comparative morphological analysis revealed that Bevismyia basuto Munro 1957 from Malawi should be included in the genus Oedosphenella. Therefore, we consider the genus name Oedosphenella Frey 1936 to be a senior synonym of Bevismyia Munro 1957 syn.n.

The purpose of this study was to determine the effects of different amounts of energy intake in combination with progressive resistance training on muscle mass and body fat in bodybuilders. Eleven male bodybuilders (26.8 ± 2.3 years, 90.1 ± 9.7 kg, and 176.9 ± 7.1 cm) were randomly assigned into one of two groups: a group that ingested higher amounts of energy (G1, 67.5 ± 1.7 kcal/kg/d, n = 6), and a group that ingested moderate amounts of energy (G2, 50.1 ± 0.51 kcal/kg/d, n = 5). Both groups performed resistance training 6 days per week over a 4-week study period. Measures of body composition were assessed before and after the intervention period. For body fat, only the G1 presented significant changes from pre- to post-training (G1 = +7.4% vs. G2 = +0.8%). For muscle mass, both groups showed significant increases after the intervention period, with G1 presenting a greater increase compared to G2 (G1 = +2.7% vs. G2 = +1.1%). Results suggest that greater energy intake in combination with resistance training induces greater increases in both muscle mass and body fat in competitive male bodybuilders., (© 2019 Alex S. Ribeiro, João Pedro Nunes, Brad J. Schoenfeld, Andreo F. Aguiar, Edilson S. Cyrino, published by Sciendo.)

The purpose of this study was to investigate the effects of BCAA supplementation on muscle recovery from resistance exercise (RE) in untrained young adults. Twenty-four young adults (24.0 ± 4.3 years old) were assigned to 1 of 2 groups (n = 12 per group): a placebo-supplement group or a BCAA-supplement group. The groups were supplemented for a period of 5 days. On day 1 and 3, both groups underwent a RE session involving two lower body exercises (hack squat and leg press) and then were evaluated for muscle recovery on the 3 subsequent moments after the RE session [30 min (day 3), 24 h (day 4), and 48 h (day 5)]. The following indicators of muscle recovery were assessed: number of repetitions, rating of perceived exertion in the last RE session, muscle soreness and countermovement jump (CMJ) during recovery period (30 min, 24 h, and 48 h after RE session). Number of repetitions remained unchanged over time (time, P > 0.05), while the rating of perceived exertion increased (time, P < 0.05) over 3 sets, with no difference between groups (group × time, P > 0.05). Muscle soreness increased (time, P < 0.05) and jumping weight decreased (time, P < 0.05) at 30 min post-exercise and then progressively returned to baseline at 24 and 48 h post-exercise, with no difference between groups (group × time, P > 0.05). The results indicate that BCAA supplementation does not improve muscle recovery from RE in untrained young adults.

The aim of this study was to evaluate the impact of 2 types of beer barrels on postural control, trunk activation, and kinematic measures in adult workers. Twelve (12) males randomly performed 4 tasks on a force platform for 20 s: (1) hold an empty recyclable barrel, (2) hold a full recyclable barrel (30 L), (3) hold an empty steel barrel, and (4) hold a full steel barrel (30 L). Trunk muscular activation, force platform and kinematic measures at the trunk, hip, and knee joints were computed. The full steel barrel produced greater postural oscillation than other conditions. Higher trunk activity was also reported during the full steel barrel task. Significant kinematic changes only in the trunk were observed between the empty steel barrel and the full recyclable barrel tasks. In conclusion, the full steel barrel produced a negative impact on postural control, increasing trunk activity and changing trunk flexion angle in adult workers.

β-Alanine supplementation has been shown to increase muscle carnosine levels and exercise performance. However, its effects on muscle recovery from resistance exercise (RE) remains unknown. The purpose of this study was to investigate the effects of β-alanine supplementation on muscle function during recovery from a single session of high-intensity RE. Twenty-four untrained young adults (22.1 ± 4.6 years old) were assigned to one of two groups (N = 12 per group): a placebo-supplement group (4.8 g/day) or an β-alanine-supplement group (4.8 g/day). The groups completed a single session of high-intensity RE after 28 days of supplementation and were then evaluated for muscle function on the three subsequent days (at 24, 48, and 72 h postexercise) to assess the time course of muscle recovery. The following indicators of muscle recovery were assessed: number of repetitions until failure, rating of perceived exertion, muscle soreness, and blood levels of creatine kinase (CK). Number of repetitions until failure increased from 24 to 48 h and 72 h of recovery (time P < 0.01), with no difference between groups. There was a significant increase in the rating of perceived exertion among the sets during the RE session (time P < 0.01), with no difference between the groups. No difference was observed over time and between groups in rating of perceived exertion in the functional tests during recovery period. Blood CK levels and muscle soreness increased at 24 h postexercise and then progressively declined at 48 and 72 h postexercise, respectively (time P < 0.05), with no difference between groups. In conclusion, our data indicate that β-alanine supplementation does not improve muscle recovery following a high-intensity RE session in untrained young adults.

Background: Chronic low back pain (CLBP) is an important disorder in athletes that may negatively affect their performance in competitions. The literature usually recommends physiotherapy based on exercises for back pain management in athletes. Recent evidence suggests that interventions based on lumbar muscle stabilisation exercises (LMSE) and back endurance-resistance exercises (BERE) may improve back pain and function performance. However, it is still unclear which type of exercise is more effective for the treatment of CLBP in athletes., Objective: To compare the efficacy of LMSE versus BERE in athletes with CLBP., Design: The study is a 2-arm, prospectively registered, randomised controlled trial., Setting: The physical therapy clinical and biomechanics laboratory of the UNOPAR University., Participants: 32 male athletes with CLBP, age between 18 and 40 years old, recruited from the local community., Intervention: An 8-week intervention programme will be carried out with LMSE s versus BERE., Measurements: Trunk neuromuscular patterns during balance tasks (unipodal and over a ball) using electromyography and force platform parameters, pain, disability, fear and avoidance will be assessed by a blinded assessor at baseline and at follow-up after 8 weeks of intervention period., Limitations: The absence of blinding intervention and the exclusion of female athletes, seated sports and swimmers will affect the internal and external validity of the study., Conclusions: The results of this study will elucidate which of these two interventions promote better results in trunk neuromuscular pattern, back pain and function in male athletes with CLBP., Competing Interests: Competing interests: None declared.

The main purpose of this study was to compare the effects of resistance training (RT) performed two versus three times per week on phase angle (a cellular health indicator) in older women. A total of 39 women (69.1 ± 5.5 years) were randomly assigned to perform a RT program two (G2X) or three (G3X) days per week for 12 weeks. The RT was a whole-body program (eight exercises, one set, 10-15 repetitions). Phase angle, resistance, reactance, and total body water were assessed by bioimpedance spectroscopy. Intracellular water, reactance, and phase angle increased significantly in G2X (2.1%, 3.0%, and 5.6%, respectively) and G3X (5.0%, 6.9%, and 10.3%, respectively) from pretraining to posttraining, with no significant difference between groups. Bioimpedance resistance decreased similarly in both groups (G2X = -1.7% vs. G3X = -3.2%). We conclude that a single set RT program with a frequency of 2 days per week may be sufficient to promote an improvement in cellular health in older women.

The purpose of this study was to investigate the effects of l-arginine supplementation on muscle recovery after a single session of high-intensity resistance exercise (RE). Twenty healthy young adult participants (22.8 ± 3.4 years old) were assigned to 1 of 2 groups (N = 10 per group): a placebo-supplement group or an l-arginine-supplement group. The groups completed a session of high-intensity RE (0 h) and 3 subsequent fatigue test sessions (at 24, 48, and 72 h postexercise) to assess the time course of muscle recovery. During the test sessions, we assessed the following dependent variables: number of maximum repetitions, electromyographic signal (i.e., root mean square (RMS) and median frequency (MF) slope), muscle soreness, perceived exertion, blood levels of creatine kinase (CK) and lactate, and testosterone:cortisol ratio. Number of maximum repetitions increased at 48 and 72 h postexercise in both groups (time, P < 0.05). CK levels and muscle soreness increased at 24 h postexercise and then progressively returned to baseline at 72 h post exercise in both groups (time, P < 0.05). Lactate levels increased immediately postexercise but were reduced at 24 h postexercise in both groups (time, P < 0.05). Testosterone:cortisol ratio, RMS, and MF slope remained unchanged during the recovery period in both groups (time, P > 0.05). No significant (P > 0.05) group × time interaction was found for all dependent variables during the recovery period. In conclusion, our data indicate that l-arginine supplementation does not improve muscle recovery following a high-intensity RE session in young adults.

Context: Strength/resistance training volume has historically been supported in the American College of Sports Medicine recommendations. However, for the back muscles, exercise prescription related to the number of sets, such as single versus multiple, is not well established in the literature., Objective: The purpose of this study was to compare the effects of 2 training volumes on strength and endurance of back-extensor muscles in untrained young participants with regard to a repeated-measures design., Design: Randomized controlled trial., Setting: Laboratory of functional evaluation and human motor performance., Participants: Forty-four untrained young participants (mean age = 21 y) were randomized into single-set (n = 14), multiple-set (MSG, n = 15), and untrained control (n = 15) groups., Intervention: The single-set group and MSG underwent a 10-week progressive resistance training program (2 d/wk) using a 45° Roman chair., Main Outcome Measures: Back maximal strength (dynamometer) and isometric and dynamic endurance (time limit, trunk extension-flexion cycles, and electromyography muscle fatigue estimates)., Results: The results showed differences between the MSG and control group for isometric endurance time (mean = 19.8 s; 95% confidence interval, -44.1 to 4.8), but without time intervention significance. Significant improvement after training (P < .05) was found predominantly during dynamic endurance (number of repetitions) for both the MSG (+61%) and single-set group (+26%) compared with preintervention, whereas the control group reported no benefit. There was no significant (P > .05) difference in either strength or electromyography estimates after training., Conclusions: Both multiple and single volume training were efficient in promoting better back endurance during dynamic performance based on mechanical variables (time and number of repetitions).

The aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0.035 cm 2 , output power 50 mW; energy density 69 J cm -2 ; exposure time 4 s per point; final energy 4.8 J). Twenty-four hours after the last application, the right and left TA muscles were collected for histological (collagen content) and molecular (gene expression of IGF-I, MyoD, MAFbx, and TNF-α) analyses, respectively. Data were analyzed using one-way ANOVA at P < 0.05. There were no significant (P > 0.05) differences in collagen density and IGF-I gene expression in all experimental groups. There were similar (P < 0.05) decreases in MAFbx and TNF-α gene expression in the injured and GaAs-injured groups, compared to control group. The MyoD gene expression increased (P = 0.008) in the GaAs-injured group, but not in the injured group (P = 0.338), compared to control group. GaAs laser therapy had a positive effect on MyoD gene expression, but not IGF-I, MAFbx, and TNF-α, during intermediary phases (14 days post-injury) of muscle repair.

Ribeiro, AS, Aguiar, AF, Schoenfeld, BJ, Nunes, JP, Cavalcanti, EF, Cadore, EL, and Cyrino, ES. Effects of different resistance training systems on muscular strength and hypertrophy in resistance-trained older women. J Strength Cond Res 32(2): 545-553, 2018-The purpose of this study was to investigate the effect of resistance training (RT) performed in a pyramid (PR) vs. constant (CT) load system on muscular strength and hypertrophy in resistance-trained older women. Thirty-three older women (69.7 ± 5.9 years, 69.1 ± 15.0 kg, 156.6 ± 6.2 cm, and 28.1 ± 5.4 kg·m) were randomized into 2 groups: one that performed RT with a CT load (n = 16) and another group that performed RT in an ascending PR fashion (n = 17). Outcomes included 1 repetition maximum (RM) tests and assessment of skeletal muscle mass estimated by dual-energy X-ray absorptiometry. The study lasted 32 weeks, with 24 weeks dedicated to preconditioning and 8 weeks for the actual experiment. The RT program was conducted 3 d·wk; the CT consisted of 3 sets of 8-12RM with same load across sets, whereas the PR consisted of 3 sets of 12/10/8RM with incremental loads for each set. A significant (p ≤ 0.05) change from pretraining to posttraining was observed for chest press total strength (CT: pre = 122.8 ± 21.0 kg, post = 128.9 ± 21.4 kg, effect size [ES] = 0.28; PR: pre = 120.5 ± 22.8 kg, post = 125.8 ± 22.9 kg, ES = 0.24) and muscle mass (CT: pre = 21.4 ± 3.6 kg, post = 21.7 ± 3.5 kg, ES = 0.09; PR: pre = 20.9 ± 3.4 kg, post = 21.1 ± 3.4 kg, ES = 0.06) without differences between groups. Results suggest that both systems are effective to improve strength and muscle growth, but PR is not superior to CT for inducing improvements in previously trained older women.

The effects of citrulline malate (CM) on muscle recovery from resistance exercise remains unknown. We aimed to determine if citrulline malate supplementation improves muscle recovery after a single session of high-intensity resistance exercise (RE) in untrained young adult men. Nine young adult men (24.0 ± 3.3 years) participated in a double-blind crossover study in which they received 6 g of CM and placebo (PL) on two occasions, separated by a seven-day washout period. Each occasion consisted of a single session of high-intensity RE (0 h) and three subsequent fatigue tests sessions (at 24, 48, and 72 h) to assess the time course of muscle recovery. During the tests sessions, we assessed the following variables: number of maximum repetitions, electromyographic signal (i.e., root mean square (RMS) and median frequency (MF)), muscle soreness and perceived exertion, as well as blood levels of creatine kinase (CK), lactate, insulin, and testosterone:cortisol ratio. CK levels increased at 24 h post-exercise and remained elevate at 48 and 72 h, with no difference between CM and PL conditions. Muscle soreness increased at 24 h post-exercise, which progressively returned to baseline at 72 h in both conditions. Lactate levels increased immediately post-exercise and remained elevated at 24, 48, and 72 h in both conditions. No significant treatment × time interaction was found for all dependents variables (maximum repetitions, perceived exertion, CK, lactate, RMS, MF, and testosterone:cortisol ratio) during the recovery period. In conclusion, our data indicate that CM supplementation (single 6 g dose pre-workout) does not improve the muscle recovery process following a high-intensity RE session in untrained young adult men., Competing Interests: The authors declare no conflict of interest.

Nitric oxide (NO) has been shown to increase skeletal muscle protein synthesis. However, the role of NO during skeletal muscle regrowth after immobilization remains unknown. The purpose of this study was to determine whether NO is required for muscle regrowth/recovery after a period of disuse by immobilization. Male Wistar rats were divided into 4 groups: recovered, 1-(2-trifluoromethyl-phenyl)-imidazole (TRIM; 10 mg·kg body mass -1 ·day -1 ), N G -nitro-l-arginine methyl ester (l-NAME; 90 mg·kg body mass -1 ·day -1 ), and control. The recovered, TRIM, l-NAME groups were submitted to a 7-d muscle recovery period (by remobilization), following a 10-d immobilization period (to induce plantaris [PLA] muscle atrophy). After the experimental period, the PLA muscle was collected for morphometrical (muscle fibers cross-sectional area [CSA]) and molecular (Phospho-mTOR Ser2448 protein expression) analysis. After 7 d of recovery, the recovered group displayed complete muscle regrowth (CSA, recovered: 2.216 ± 214 vs., Control: 2.219 ± 280 cm 2 ; P > 0.05). However, CSA of the l-NAME (1.911 ± 267 cm 2 ) and TRIM (1.896 ± 219 cm 2 ) groups were statistically (P < 0.05) lower than the recovered and control groups. Additionally, there was a 29% increase in Phos-mTOR Ser2448 protein expression levels in the recovered group compared to control group, and this increase was blocked in both TRIM and l-NAME groups. In conclusion, our results indicate that NO is crucial for skeletal muscle regrowth after an immobilization period, potentially via the mTOR signaling pathway., (Copyright © 2017 Elsevier Inc. All rights reserved.)

The purpose of this study was to examine the effects of free leucine supplementation on changes in skeletal muscle mass and strength during a resistance training (RT) program in previously untrained, young subjects. In a double-blind, randomized, placebo-controlled study, 20 healthy young (22 ± 2 years) participants were assigned to two groups: a placebo-supplement group (PLA, N = 10) or a leucine-supplement group (LEU, N = 10). Both groups underwent an 8-week hypertrophic RT program (2 days/week), consuming an equivalent amount of leucine (3.0 g/day in a single post-training dose) or placebo (cornstarch). Quadriceps muscle strength, cross-sectional area (CSA) of the vastus lateralis (VL), and rectus femoris (RF), as well as the habitual dietary intake were assessed before and after the 8-week intervention period. There was a similar improvement in muscle strength (Leg press, LEU: +33% vs. PLA: +37%; P > 0.05, and knee extension, LEU: +31% vs. PLA: 34%; P > 0.05) and CSA (VL, LEU: 8.9% vs. PLA: 9.6%; P > 0.05, and RF, LEU: +21.6% vs. PLA: + 16.4%; P > 0.05) in the both groups from pre- to post-training. In addition, there was no significant (P > 0.05) difference in daily dietary intake between the LEU and PLA groups before and after the intervention period. Free leucine supplementation (3.0 g/day post-training) does not increase muscle strength or CSA during RT in healthy young subjects consuming adequate dietary protein intake.

Cardiotonic drugs and exercise training promote cardiac inotropic effects, which may affect training-induced cardiac adaptations. This study investigated the effects of long-term administration of digoxin on heart structure and function, and physical performance of rats submitted to high-intensity interval training (HIIT). Male Wistar rats, 60 days old, were divided into control (C), digoxin (DIGO), trained (T), and trained with digoxin (TDIGO). Digoxin was administered by gavage (30 µg/kg/day) for 75 days. The HIIT program consisted of treadmill running 60 min/day (8 min at 80% of the maximum speed (MS) and 2 min at 20% of the MS), 5 days per week during 60 days. The main cardiac parameters were evaluated by echocardiograph and cardiomyocyte area was determined by histology. There were no group x time effects of digoxin, HIIT or interactions (digoxin and HIIT) on functional echocardiographic parameters (heart rate; ejection fraction) or in the maximum exercise test. There was a group x time interaction, as evidenced by observed cardiac hypertrophy in the TDIGO group evaluated by ratio of left ventricle weight to body weight (p<0.002) and cardiomyocyte area (p<0.000002). Long-term administration of digoxin promoted cardiac hypertrophy without affecting cardiac function and physical performance in rats submitted to HIIT., (© Georg Thieme Verlag KG Stuttgart · New York.)

Purpose: The purpose of this study was to examine the effects of acute L-arginine (L-arg) supplementation on peripheral vasodilatation and muscle performance in older women., Methods: In a double-blind, randomized, placebo-controlled study, 20 elderly women were randomly assigned in a double-blind fashion to either an L-arg (ARG, N = 10) or placebo (PLA, N = 10) group. During the first visit, both groups underwent a Doppler ultrasound exam (to assess the femoral artery vasodilatation) at rest (baseline), and immediately before and after the isokinetic strength test (performed at 80 min after supplementation). On the second and third visits, the groups completed a battery of muscle performance tests (to assess the isometric and functional strength), initiated at the same time point (80 min after supplementation)., Results: The femoral artery blood flow (ARG: 443.9 ± 42.8 vs. PLA: 373.1 ± 40.8 ml/min; P > 0.05) and area (ARG: 0.45 ± 0.03 vs. PLA: 0.41 ± 0.02 cm(2); P > 0.05) were similar between the groups at basal conditions, and they remained unchanged after supplementation. Following exercise, blood flow increased ~160 % above the basal level, and there was no significant (P > 0.05) difference between the ARG and PLA groups. Additionally, there were no significant (P > 0.05) differences between the ARG and PLA groups for any strength variable (isokinetic, isometric, and functional)., Conclusion: These results show that acute L-arg supplementation provides no ergogenic effect on blood flow and muscle performance in older women.

New Findings: What is the central question of this study? We investigated the effects of physical training on phenotypic (fibre-type content) and myogenic features (MyoD and myogenin expression) in skeletal muscle during the transition from cardiac hypertrophy to heart failure. What is the main finding and its importance? We provide new insight into skeletal muscle adaptations by showing that physical training increases the type I fibre content during the transition from cardiac hypertrophy to heart failure, without altering MyoD and myogenin expression. These results have important clinical implications for patients with heart failure, because this population has reduced muscle oxidative capacity. The purpose of this study was to investigate the effects of physical training (PT) on phenotypic features (fibre-type content) and myogenic regulatory factors (MyoD and myogenin) in rat skeletal muscle during the transition from cardiac hypertrophy to heart failure. We used the model of ascending aortic stenosis (AS) to induce heart failure in male Wistar rats. Sham-operated animals were used as age-matched controls. At 18 weeks after surgery, rats with ventricular dysfunction were randomized into the following four groups: sham-operated, untrained (Sham-U; n = 8); sham-operated, trained (Sham-T; n = 6); aortic stenosis, untrained (AS-U; n = 6); and aortic stenosis, trained (AS-T; n = 8). The AS-T and Sham-T groups were submitted to a 10 week aerobic PT programme, while the AS-U and Sham-U groups remained untrained for the same period of time. After the PT programme, the animals were killed and the soleus muscles collected for phenotypic and molecular analyses. Physical training promoted type IIa-to-I fibre conversion in the trained groups (Sham-T and AS-T) compared with the untrained groups (Sham-U and AS-U). No significant (P > 0.05) differences were found in type I or IIa fibre content in the AS-U group compared with the Sham-U group. Additionally, there were no significant (P > 0.05) differences in the myogenic regulatory factors MyoD and myogenin (gene and protein) expression between the groups. Therefore, our results indicate that PT may be a suitable strategy to improve the oxidative phenotype in skeletal muscle during the transition from cardiac hypertrophy to heart failure, without altering MyoD and myogenin., (© 2016 The Authors. Experimental Physiology © 2016 The Physiological Society.)

Physical training has been shown to be important to the control of muscle mass during aging, through the activation of several pathways including, IGF1-AKT and PGC-1α. Also, it was demonstrated that LRP130, a component of the PGC-1α complex, is important for the PGC-1α-dependent transcription of several mitochondrial genes in vivo. To explore the role of physical training during aging, we investigated the effects on muscle recovery after short-term immobilization followed by 3 or 7 days with aerobic or resistance training. Using morphological (myofibrillar adenosine triphosphatase activity, to assess the total muscle fiber cross-sectional area (CSA) and the frequency of specific fiber types), biochemical (myosin heavy chain), and molecular analyses (quantitative real-time PCR, functional pathways analyses, and Western blot), our results indicated that after an atrophic stimulus, only animals subjected to aerobic training showed entire recovery of cross-sectional area; aerobic training reduced the ubiquitin-proteasome system components involved in muscle atrophy after 3 days of recovery, and the upregulation in PGC-1α expression enhanced the process of muscle recovery by inhibiting the FoxO pathway, with the possible involvement of LRP130. These results suggest that aerobic training enhanced the muscle regeneration process after disuse-induced atrophy in aged rats possibly through of the LRP130/PGC-1α complex by inhibiting the ubiquitin-proteasome system., (© Crown copyright 2015.)

Background: Although the beneficial effects of resistance training (RT) on the cardiovascular system are well established, few studies have investigated the effects of the chronic growth hormone (GH) administration on cardiac remodeling during an RT program., Objective: To evaluate the effects of GH on the morphological features of cardiac remodeling and Ca2+ transport gene expression in rats submitted to RT., Methods: Male Wistar rats were divided into 4 groups (n = 7 per group): control (CT), GH, RT and RT with GH (RTGH). The dose of GH was 0.2 IU/kg every other day for 30 days. The RT model used was the vertical jump in water (4 sets of 10 jumps, 3 bouts/wk) for 30 consecutive days. After the experimental period, the following variables were analyzed: final body weight (FBW), left ventricular weight (LVW), LVW/FBW ratio, cardiomyocyte cross-sectional area (CSA), collagen fraction, creatine kinase muscle-brain fraction (CK-MB) and gene expressions of SERCA2a, phospholamban (PLB) and ryanodine (RyR)., Results: There was no significant (p > 0.05) difference among groups for FBW, LVW, LVW/FBW ratio, cardiomyocyte CSA, and SERCA2a, PLB and RyR gene expressions. The RT group showed a significant (p < 0.05) increase in collagen fraction compared to the other groups. Additionally, the trained groups (RT and RTGH) had greater CK-MB levels compared to the untrained groups (CT and GH)., Conclusion: GH may attenuate the negative effects of RT on cardiac remodeling by counteracting the increased collagen synthesis, without affecting the gene expression that regulates cardiac Ca2+ transport.

Background: The purpose of this study was to examine the effects of an additional set of exhaustive exercise before local muscular endurance (LME) training on maximal dynamic strength and endurance of quadriceps muscle in young men., Methods: Twenty-seven healthy men (age: 20.9±1.8 years) performed one repetition maximum (1RM), muscular endurance, and magnetic resonance imaging (MRI) tests on two separate occasions (before and after an 8-wk LME training program using a bilateral knee extensor machine). After baseline testing, the subjects were divided into three groups: untrained control (CO, N.=9), traditional training (TR, N.=9), and prior exhaustive training (PE, N.=9). Both the TR and PE groups trained using the same LME training protocol (2 d∙wk-1; 3 sets of 15-20 repetitions at 50% of 1RM) throughout the 8-wk experimental period; the only difference was that the PE group performed an additional set of exhaustive exercise at 80% of 1RM immediately before each training session., Results: After 8 wk, the PE group experienced a greater (P<0.05) increase in 1RM, endurance, and work efficiency than the TR group. Additionally, no changes (P>0.05) in cross-sectional area (CSA), body mass and daily dietary intake were observed from pre- to post-test in either group., Conclusions: These results suggest that the inclusion of a single set of exhaustive exercise at 80% of 1RM immediately before LME training can be a suitable strategy for inducing additional beneficial effects on quadriceps strength and endurance in young men.

The purpose of this study was to compare back muscle fatigue of younger and older participants with and without chronic low back pain (CLBP). Twenty participants without and 20 with nonspecific CLBP participated in this study. Each group contained 10 younger (50% males; mean age: 31 ± 6 yrs) and 10 older adults (50% males; age mean: 71 ± 7 yrs). Two isometric fatigue protocols were presented randomly: (1) to maintain the unsupported trunk at the horizontal position while on a 45° Roman chair for a minute, and (2) to maintain a 10% of body weight box close to the trunk in the upright position for a minute. Surface electromyography (EMG) signals from the back (multifidus and iliocostalis) and one hip (biceps femoris) muscles were recorded bilaterally, and the median frequency fatigue estimate from linear regression slopes of the EMG time-series was computed. There were no significant (P > 0.05) age effects, and group-by-age interaction in both isometric and functional fatigue tasks. However, the CLBP groups (both younger and old) displayed more back fatigue than people without CLBP in both fatigue protocols (P < 0.01; effect size varying of d = 0.17-0.32). This study was sensitive to discriminate that individuals with CLBP did present significantly more pronounced EMG back fatigue than people without CLBP, in both younger and older adults. These results have significant clinical implications for low back pain rehabilitation programs with regard to endurance assessment in both younger and older., (Copyright © 2015 Elsevier Ltd. All rights reserved.)