1. Agrobacterium leguminum sp. nov., isolated from nodules of Phaseolus vulgaris in Spain.
- Author
-
Castellano-Hinojosa A, Correa-Galeote D, Ramírez-Bahena MH, Tortosa G, González-López J, Bedmar EJ, and Peix Á
- Subjects
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial genetics, Fatty Acids chemistry, Nucleic Acid Hybridization, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Spain, Agrobacterium classification, Agrobacterium isolation & purification, Phaseolus microbiology, Phylogeny, Root Nodules, Plant microbiology
- Abstract
Two endophytic strains, coded MOVP5
T and MOPV6, were isolated from nodules of Phaseolus vulgaris plants grown on agricultural soil in Southeastern Spain, and were characterized through a polyphasic taxonomy approach. Their 16S rRNA gene sequences showed 99.3 and 99.4 %, 98.9 and 99.6 %, and 99.0 and 98.7% similarity to ' A. deltaense ' YIC 4121T , A. radiobacter LGM 140T , and A. pusense NRCPB10T , respectively. Multilocus sequence analysis based on sequences of recA and atpD genes suggested that these two strains could represent a new Agrobacterium species with less than 96.5 % similarity to their closest relatives. PCR amplification of the telA gene, involved in synthesis of protelomerase, confirmed the affiliation of strains MOPV5T and MOPV6 to the genus Agrobacterium . Whole genome average nucleotide identity and digital DNA-DNA hybridization average values were less than 95.1 and 66.7 %, respectively, with respect to its closest related species. Major fatty acids in strain MOPV5T were C18 : 1 ω7 c /C18 : 1 ω6 c in summed feature 8, C19 : 0 cyclo ω8 c , C16 : 0 and C16 : 0 3-OH. Colonies were small to medium, pearl-white coloured on YMA at 28 °C and growth was observed at 10-42 °C, pH 5.0-10.0 and with 0.0-0.5 % (w/v) NaCl. The DNA G+C content was 59.9 mol%. These two strains differ from all other genomovars of Agrobacterium found so far, including those that have not yet given a Latin name. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strain MOPV5T as representing a novel species of Agrobacterium , for which the name Agrobacterium leguminum sp. nov. is proposed. The type strain is MOPV5T (=CECT 30096T =LMG 31779T ).- Published
- 2021
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