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Thrombosis is characterized by the formation of clots in the blood vessels. Antithrombin-III deficiency in the blood causes thrombus formation. Supplementing antithrombin-III may serve as anticoagulant therapy. In the present studies, an antithrombin like Protein from Punica granatum has been identified and characterized using in silico approach. Based on sequence homology, an ALPP was selected depending upon its highest binding affinity of -41.28 kcal/mol with thrombin. Thrombin structure complexed with ALPP was docked with TAME using AutoDock Vina. No binding was observed for TAME at Ser195 of thrombin. MD simulation (50 ns) was performed to evaluate the flexibility and stability of docked complexes. In vitro assays with crude protein showed 78% thrombin inhibition at 5 μg and calculated IC50 value was 0.188 μg. The presence of thrombin inhibitors in crude protein was also confirmed by reverse zymography. Thus, it is very likely that the protein identified from P. granatum may act as thrombin inhibitor. [ABSTRACT FROM AUTHOR]

Byline: Barnita. Saha, Isha. Gupta, Kamal. Aggarwal, Sant. Kataria Dear Editor, A 25-year-old unmarried man presented with multiple asymptomatic skin colored to reddish-purple raised skin lesions on the shaft of [...]

Background: Vitamin D analogues and NBUVB are both well‐recognised modes of therapy in the treatment of chronic stable plaque psoriasis. The objective of this open label intraindividual, left right study was to compare two different vitamin D analogues, calcipotriol and calcitriol, in combination with NBUVB phototherapy in psoriasis. Methods: Thirty patients with stable plaque psoriasis were enrolled for a 12‐week clinical trial. The target lesion on the left side was treated topically with calcitriol ointment, while that on the right side was treated with calcipotriol ointment once daily. The whole body was irradiated with narrow‐band ultraviolet B phototherapy (NBUVB) three times per week. Efficacy was assessed by target plaque scoring. Results: Both therapies resulted in a statistically significant reduction in erythema, scaling, thickness, and target plaque score, seen as early as 2 weeks into therapy. However, the calcipotriol combination led to an earlier clearance of plaques and a lesser relapse rate than the calcitriol combination. The number of treatment sessions and cumulative NBUVB doses were significantly lower in the calcipotriol‐treated group. Conclusion: Both vitamin D analogues appear to be safe, effective, and cosmetically acceptable, with calcipotriol being more efficacious, well tolerated, with a rapid onset of action and a better maintenance of response. [ABSTRACT FROM AUTHOR]

Byline: Sarabjit. Kaur, Parul. Aggarwal, Ankita. Sangwan, Kamal. Aggarwal, Surabhi. Dayal, Vijay. JainAn 8-year-old boy, born to nonconsanguineous healthy parents, presented with a history of developing asymptomatic spontaneous erosions over [...]

Thrombosis, a life-threatening disease causes thrombus formation within blood vessels to obstruct the flow of blood through vascular system. The disease causes morbidity and mortality of arterial and venous thrombosis which can result in acute infraction and deep vein thrombosis. Majority of clotting factors are serine proteases and are present as precursors of proteolytic enzymes known as zymogens that circulate in inactivated forms but get activated in response to injury in blood vessels. The serine proteases are involved in coagulation, anticoagulation, and fibrinolysis. Thrombin and fibrin are the main key therapeutic target for the treatment of thrombosis. Commercially available antithrombotic and thrombolytic drugs are known to be associated with serious side effects like bleeding, hypertension, and haemorrhage therefore natural products have been suggested as best replacement of synthetic antithrombotic and thrombolytic drugs with less side effects for human health. Plants have been suggested as potential source of anticoagulants against thrombosis with fewer or no side effects. [ABSTRACT FROM AUTHOR]

Byline: Jyoti. Sharma, Jyoti. Kaushal, Kamal. Aggarwal Background: Tinea versicolor (TV) is characterised by the appearance of maculosquamous lesions sometimes associated with mild erythema and pruritus in characteristic areas of [...]

Lupus vulgaris is the most common form of cutaneous tuberculosis occurring in previously sensitized individuals with a high degree of tuberculin sensitivity. Various forms including plaque, ulcerative, hypertrophic, vegetative, papular, and nodular forms have been described. A 30-year-old male patient presented with a very large hypertrophic lupus vulgaris lesion over left side of chest since 22 years. Histopathological examination showed granulomatous infiltration without caseation necrosis. The Mantoux reaction was strongly positive. Hypertrophic lupus vulgaris of such a giant size and that too at an unusual site is extremely rare and hence is being reported.

Byline: Ankita. Sangwan, Sarabjit. Kaur, Kamal. Aggarwal, Ishita. Dua Sir, Sarcoidosis is a multisystem granulomatous disorder of unknown etiology, mostly encountered in adults aged 20–40 years.[sup][1] Childhood sarcoidosis is a [...]

Byline: Parul. Aggarwal, Kamal. Aggarwal, Sarabjit. Kaur, Vijay. Jain Sir, Amyloidosis refers to a group of disorders characterized by extracellular deposition of insoluble beta-pleated protein fibrils in various organs.[sup][1] Cutaneous [...]