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219 results on '"Agasti, Sarit S."'

12. Autofluorescence-spectral imaging for rapid and invasive characterization of soybean for pre-germination anaerobic stress tolerance.

Author

Rajendran, Ambika, Ramlal, Ayyagari, Sarkar, Subham, Agasti, Sarit S., Rajarajan, K., Lal, S. K., Raju, Dhandapani, and Subramaniam, Sreeramanan

Subjects
BIOFLUORESCENCE, LIGHT absorption, SOYBEAN, FLUOROPHORES
Abstract

The autofluorescence-spectral imaging (ASI) technique is based on the lightemitting ability of natural fluorophores. Soybean genotypes showing contrasting tolerance to pre-germination anaerobic stress can be characterized using the photon absorption and fluorescence emission of natural fluorophores occurring in seed coats. In this study, tolerant seeds were efficiently distinguished from susceptible genotypes at 405 nm and 638 nm excitation wavelengths. ASI approach can be employed as a new marker for the detection of photonemitting compounds in the tolerant and susceptible soybean seed coats. Furthermore, the accuracy of rapid characterization of genotypes using this technique can provide novel insights into soybean breeding. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Cucurbit[7]uril Macrocyclic Sensors for Optical Fingerprinting: Predicting Protein Structural Changes to Identifying Disease-Specific Amyloid Assemblies

Author

Das Saha, Nilanjana, primary, Pradhan, Soumen, additional, Sasmal, Ranjan, additional, Sarkar, Aritra, additional, Berač, Christian M., additional, Kölsch, Jonas C., additional, Pahwa, Meenakshi, additional, Show, Sushanta, additional, Rozenholc, Yves, additional, Topçu, Zeki, additional, Alessandrini, Vivien, additional, Guibourdenche, Jean, additional, Tsatsaris, Vassilis, additional, Gagey-Eilstein, Nathalie, additional, and Agasti, Sarit S., additional

Published
2022
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20. Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues

Author

Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Media Laboratory, Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences, Wang, Yu, Woehrstein, Johannes B., Donoghue, Noah, Dai, Mingjie, Avendaño, Maier S., Schackmann, Ron C. J., Zoeller, Jason J., Wang, Shan Shan H., Tillberg, Paul W., Park, Demian, Lapan, Sylvain W., Boyden, Edward, Brugge, Joan S., Kaeser, Pascal S., Church, George M., Agasti, Sarit S., Jungmann, Ralf, Yin, Peng, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Media Laboratory, Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences, Wang, Yu, Woehrstein, Johannes B., Donoghue, Noah, Dai, Mingjie, Avendaño, Maier S., Schackmann, Ron C. J., Zoeller, Jason J., Wang, Shan Shan H., Tillberg, Paul W., Park, Demian, Lapan, Sylvain W., Boyden, Edward, Brugge, Joan S., Kaeser, Pascal S., Church, George M., Agasti, Sarit S., Jungmann, Ralf, and Yin, Peng

Abstract

To decipher the molecular mechanisms of biological function, it is critical to map the molecular composition of individual cells or even more importantly tissue samples in the context of their biological environment in situ. Immunofluorescence (IF) provides specific labeling for molecular profiling. However, conventional IF methods have finite multiplexing capabilities due to spectral overlap of the fluorophores. Various sequential imaging methods have been developed to circumvent this spectral limit but are not widely adopted due to the common limitation of requiring multirounds of slow (typically over 2 h at room temperature to overnight at 4 °C in practice) immunostaining. We present here a practical and robust method, which we call DNA Exchange Imaging (DEI), for rapid in situ spectrally unlimited multiplexing. This technique overcomes speed restrictions by allowing for single-round immunostaining with DNA-barcoded antibodies, followed by rapid (less than 10 min) buffer exchange of fluorophore-bearing DNA imager strands. The programmability of DEI allows us to apply it to diverse microscopy platforms (with Exchange Confocal, Exchange-SIM, Exchange-STED, and Exchange-PAINT demonstrated here) at multiple desired resolution scales (from ∼300 nm down to sub-20 nm). We optimized and validated the use of DEI in complex biological samples, including primary neuron cultures and tissue sections. These results collectively suggest DNA exchange as a versatile, practical platform for rapid, highly multiplexed in situ imaging, potentially enabling new applications ranging from basic science, to drug discovery, and to clinical pathology.

Published
2020

29. Self-Sorted, Random, and Block Supramolecular Copolymers via Sequence Controlled, Multicomponent Self-Assembly

Author

Sarkar, Aritra, primary, Sasmal, Ranjan, additional, Empereur-mot, Charly, additional, Bochicchio, Davide, additional, Kompella, Srinath V. K., additional, Sharma, Kamna, additional, Dhiman, Shikha, additional, Sundaram, Balasubramanian, additional, Agasti, Sarit S., additional, Pavan, Giovanni M., additional, and George, Subi J., additional

Published
2020
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36. Synthesis and Localized Photoluminescence Blinking of Lead‐Free 2D Nanostructures of Cs3Bi2I6Cl3 Perovskite.

Author

Kundu, Kaushik, Acharyya, Paribesh, Maji, Krishnendu, Sasmal, Ranjan, Agasti, Sarit S., and Biswas, Kanishka

Subjects
PHOTOLUMINESCENCE, HIGH resolution imaging, OPTICAL properties, MICROSCOPY, NANOSTRUCTURES, FLUORESCENCE microscopy
Abstract

Two‐dimensional (2D) lead‐free halide perovskites have generated enormous perception in the field of optoelectronics due to their fascinating optical properties. However, an in‐depth understanding on their shape‐controlled charge‐carrier recombination dynamics is still lacking, which could be resolved by exploring the photoluminescence (PL) blinking behaviour at the single‐particle level. Herein, we demonstrate, for the first time, the synthesis of nanocrystals (NCs) and 2D nanosheets (NSs) of layered mixed halide, Cs3Bi2I6Cl3, by solution‐based method. We applied fluorescence microscopy and super‐resolution optical imaging at single‐particle level to investigate their morphology‐dependent PL properties. Narrow emission line widths and passivation of non‐radiative defects were evidenced for 2D layered nanostructures, whereas the activation of shallow trap states was recognized at 77 K. Interestingly, individual NCs were found to display temporal intermittency (blinking) in PL emission. On the other hand, NS showed temporal PL intensity fluctuations within localized domains of the crystal. In addition, super‐resolution optical image of the NS from localization‐based method showed spatial inhomogeneity of the PL intensity within perovskite crystal. [ABSTRACT FROM AUTHOR]

Published
2020
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41. Intracellular delivery of a membrane-impermeable enzyme in active form using functionalized gold nanoaprticles

Author

Ghosh, Partha, Xiaochao Yang, Arvizo, Rochelle, Zheng-Jiang Zhu, Agasti, Sarit S., Zhihong Mo, and Rotello, Vincent M.

Subjects
Biological transport -- Analysis, Gold -- Chemical properties, Gold -- Electric properties, Membrane proteins -- Structure, Membrane proteins -- Chemical properties, Chemistry
Abstract

Gold nanoparticles are coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Microscopy and enzyme assays have shown that the particles are able to transport functional enzymes into a variety of cell lines and the transported proteins are able to escape from endosomes.

Published
2010

42. Photoregulated release of caged anticancer drugs from gold nanoparticles

Author

Agasti, Sarit S., Chompoosor, Apiwat, Chang-Cheng You, Ghosh, Partha, Chae Kyu Kim, and Rotello, Vincent M.

Subjects
Gold compounds -- Structure, Gold compounds -- Chemical properties, Nanoparticles -- Structure, Nanoparticles -- Chemical properties, Antimitotic agents -- Chemical properties, Antineoplastic agents -- Chemical properties, Ultraviolet radiation -- Analysis, Chemistry
Abstract

An anticancer drug (5-fluorouracil) is conjugated to the surface of gold nanoparticles through a photocleavable o-nitrobenzyl linkage. The particles have served as both cage and carrier for the therapeutic and have provided a nontoxic conjugate that has released the payload upon long wavelength ultraviolet irradiation.

Published
2009

44. Self-assembly and cross-linking of FePt nanoparticles at planar and colloidal liquid-liquid interfaces

Author

Arumugam, Palaniappan, Patra, Debabrata, Samanta, Bappaditya, Agasti, Sarit S., Subramani, Chandramouleeswaran, and Rotello, Vincent M.

Subjects
Iron compounds -- Chemical properties, Iron compounds -- Structure, Platinum compounds -- Chemical properties, Platinum compounds -- Structure, Liquid chromatography -- Analysis, Nanoparticles -- Chemical properties, Nanoparticles -- Structure, Chemistry
Abstract

The cross-linked FePt nanoparticles (NPs) at the liquid-liquid interface using coordination chemistry were employed as an alternate route to fabricate stable colloidal shells and membranes. The stable water-in-oil colloidal shells and membranes could be manipulated by using external magnetic fields to provide a convenient way to arrange such materials.

Published
2008

45. Modulation of the catalytic behavior of [alpha]-chymotrypsin at monolayer-protected nanoparticle surfaces

Author

Chang-Cheng You, Agasti, Sarit S., De, Mrinmoy, Knapp, Michael J., and Rotello, Vincent M.

Subjects
Catalysis -- Research, Chymotrypsin -- Research, Nanoparticles -- Chemical properties, Gold compounds -- Chemical properties, Chemistry
Abstract

The enzymatic kinetics of ChT upon binding to amino-acid-functionalized gold nanoparticles toward different substrates is investigated and it is demonstrated that the complex formation provides a powerful tool to tune the enzyme specificity. The association of ChT with anionic nanoparticles leads to the increase of specificity to the positively charged substrate and the decrease of specificity to the negatively charged substrates.

Published
2006

46. Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues

Author

Wang, Yu, primary, Woehrstein, Johannes B., additional, Donoghue, Noah, additional, Dai, Mingjie, additional, Avendaño, Maier S., additional, Schackmann, Ron C. J., additional, Zoeller, Jason J., additional, Wang, Shan Shan H., additional, Tillberg, Paul W., additional, Park, Demian, additional, Lapan, Sylvain W., additional, Boyden, Edward S., additional, Brugge, Joan S., additional, Kaeser, Pascal S., additional, Church, George M., additional, Agasti, Sarit S., additional, Jungmann, Ralf, additional, and Yin, Peng, additional

Published
2017
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47. Rapid Sequential in Situ Multiplexing With DNA-Exchange-Imaging

Author

Wang, Yu, primary, Woehrstein, Johannes B., additional, Donoghue, Noah, additional, Dai, Mingjie, additional, Avendaño, Maier S., additional, Schackmann, Ron C.J., additional, Zoeller, Jason J., additional, Wang, Shan Shan H., additional, Tillberg, Paul W., additional, Park, Demian, additional, Lapan, Sylvain W., additional, Boyden, Edward S., additional, Brugge, Joan S., additional, Kaeser, Pascal S., additional, Church, George M., additional, Agasti, Sarit S., additional, Jungmann, Ralf, additional, and Yin, Peng, additional

Published
2017
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49. Quantitative super-resolution imaging with qPAINT

Author

Jungmann, Ralf, primary, Avendaño, Maier S, additional, Dai, Mingjie, additional, Woehrstein, Johannes B, additional, Agasti, Sarit S, additional, Feiger, Zachary, additional, Rodal, Avital, additional, and Yin, Peng, additional

Published
2016
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