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2. Biosynthesis and characterization of silver nanoparticles from Cedrela toona leaf extracts: An exploration into their antibacterial, anticancer, and antioxidant potential

Author

Javed Muhammad Ammar, Ali Baber, Sarfraz Muhammad Hassan, Ali Sikander, Liaqat Erum, Afzal Muhammad Sohail, Wang Yanting, Peng Liangcai, ur Rehman Asad, Aftab Muhammad Nauman, Alarjani Khaloud Mohammed, and Elshikh Mohamed Soliman

Subjects
agno3, silver nanoparticles, biofabrication, biosynthesis, antioxidant, Chemistry, QD1-999
Abstract

This research work aims to synthesize environmentally benign and cost-effective metal nanoparticles. In this current research scenario, the leaf extract of Cedrela toona was used as a reducing agent to biosynthesize silver nanoparticles (AgNPs). The synthesis of AgNPs was confirmed by the color shift of the reaction mixture, i.e., silver nitrate and plant extract, from yellow to dark brown colloidal suspension and was established by UV-visible analysis showing a surface plasmon resonance band at 434 nm. Different experimental factors were optimized for the formation and stability of AgNPs, and the optimum conditions were found to be 1 mM AgNO3 concentration, a 1:9 ratio of extract/precursor, and an incubation temperature of 70°C for 4 h. The Fourier transform infrared spectroscopy spectra indicated the presence of phytochemicals in the leaf extract that played the role of bioreducing agents in forming AgNPs. X-ray diffraction patterns confirmed the presence of AgNPs with a mean size of 25.9 nm. The size distribution and morphology of AgNPs were investigated by scanning electron microscopy, which clearly highlighted spherical nanoparticles with a size distribution of 22–30 nm with a mean average size of 25.5 nm. Moreover, prominent antibacterial activity was found against Enterococcus faecalis (21 ± 0.5 mm), Bacillus subtilis (20 ± 0.9 mm), Pseudomonas aeruginosa (18 ± 0.3 mm), Staphylococcus aureus (16 ± 0.7 mm), Klebsiella pneumoniae (16 ± 0.3 mm), and Escherichia coli (14 ± 0.7 mm). In addition, antioxidant activity was determined by DPPH and ABTS assays. Higher antioxidant activity was reported in AgNPs compared to the plant extract in both DPPH (IC50 = 69.62 µg·ml−1) and ABTS assays (IC50 = 47.90 µg·ml−1). Furthermore, cytotoxic activity was also investigated by the MTT assay against MCF-7 cells, and IC50 was found to be 32.55 ± 0.05 µg·ml−1. The crux of this research is that AgNPs synthesized from the Cedrela toona leaf extract could be employed as antibacterial, antioxidant, and anticancer agents for the treatment of bacterial, free radical-oriented, and cancerous diseases.

Published
2024
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11. Heterologous expression and characterization of a novel thermostable and alkali stable recombinant lipase enzyme from Bacillus thuringensis into E. coliBL21(DE3) for detergent formulation

Author

Zafar, Asma, primary, Rahman, Ziaur, additional, Hamid, Attia, additional, Sughra, Fatima, additional, Makhdoom, Madood, additional, Fatima, Samreen, additional, Ahmed, Haseeb, additional, Mehmood, Zeenat, additional, Khan, Maryam, additional, and Aftab, Muhammad Nauman, additional

Published
2024
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17. Impact of Locally Characterized Protease from Geobacillus SBS 4S on the Growth of Poultry Bird.

Author

Ahmad, Waheed, Tayyab, Muhammad, Muneer, Bushra, Hashmi, Abu Saeed, Ahmad, Mansurud Din, Saeed, Shagufta, Aftab, Muhammad Nauman, Firyal, Sehrish, Wasim, Muhammad, Azam, Muhammad, Talha, Muhammad, and Awan, Ali Raza

Abstract

Present study was conducted to determine the efficacy of novel thermostable protease in poultry production. The protease was produced from Geobacillus sp. SBS-4S using Luria Bertani medium and it was used as supplement in poultry feed trial. The data were statistically analyzed by Multivariate Analysis of Variance. For feeding, 150 day-old broiler chicks were divided randomly into 5 groups having 30 chicks each. Group A served as negative control, group B, C and D as experimental groups which were supplemented in the basal diet with 2500, 5000 and 7500 IU/kg of locally characterized thermostable protease while group E was supplemented with the commercially available neutral protease (5000 IU/ kg) that served as a positive control. The trial lasted for five weeks and during the trial period birds were offered ad libitum access to water and feed. The maximal weight gain was recorded when the feed was supplemented with 7500 IU/Kg of locally characterized protease. The evaluation of data of various groups obtained after 3rd, 4th and 5th weeks of trial demonstrated significant change (P≤ 0.05) in weight gain, feed up take and feed conversion ratio (FCR) as compared to negative control. Locally produced protease showed promising results as compared to positive control which is being used currently in the poultry industry. The weight gain, feed intake and feed conversion ratio of 1891.54g, 3750g and 1.98 of experimental group D were comparable to 1874.49g, 3740 g and 1.995 in case of positive control. The ability of protease to enhance weight gain, feed consumption and FCR in poultry birds makes it a strong candidate for the replacement of imported counterpart being utilized currently in poultry feed industry. [ABSTRACT FROM AUTHOR]

Published
2024
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18. In Vitro Biofilm-Mediated Biodegradation of Pesticides and Dye-Contaminated Effluents Using Bacterial Biofilms

Author

Liaqat, Iram, primary, Khalid, Awais, additional, Rubab, Saima, additional, Rashid, Farzana, additional, Latif, Asma Abdul, additional, Naseem, Sajida, additional, Bibi, Asia, additional, Khan, Bushra Nisar, additional, Ansar, Waiza, additional, Javed, Arshad, additional, Afzaal, Muhammad, additional, Summer, Muhammad, additional, Majid, Samia, additional, Ali, Sikander, additional, and Aftab, Muhammad Nauman, additional

Published
2023
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19. List of contributors

Author

Abrar, Shazia, primary, Aftab, Muhammad Nauman, additional, Ahmad, Abrar, additional, Ahmad, Mirza Nadeem, additional, Ahmad, Mudasir, additional, Ahmad, Muhammad Shaheez, additional, Ahmad, Suhail, additional, Akbar, Muhammad Usman, additional, Akhtar, Maria, additional, Akram, Nadia, additional, Akram, Nudrat Aisha, additional, Alay-e-Abbas, Syed Muhammad, additional, Ali, Adnan, additional, Ali, Irfan, additional, Ali, Muhammad, additional, Amin, Nasir, additional, Anjum, Muhammad Naveed, additional, Anwar, Farooq, additional, Arshad, Muhammad Imran, additional, Arshad, Muhammad Umair, additional, Asgher, Muhammad, additional, Ashraf, Muhammad, additional, Asim, Sadia, additional, Aslam, Nosheen, additional, Athar, Muhammad Makshoof, additional, Barikani, Mehdi, additional, Bhatti, Haq Nawaz, additional, Bhatti, Ijaz Ahmad, additional, Bilal, Choudhary Haseeb, additional, Bokhari, Tanveer Hussain, additional, Dar, Ovas Ahmad, additional, Dong, Yu, additional, Farrukh, Muhammad Akhyar, additional, Ghaffar, Saba, additional, Hafeez, Iram, additional, Hasan, Muhammad Sajjad, additional, Hashmi, Athar Adil, additional, Huma, Zill-e, additional, Hussain, Rizwan, additional, Ibrahim, Muhammad, additional, Ikram, Saiqa, additional, Iqbal, Irfana, additional, Iqbal, Muhammad, additional, Iqbal, Naeem, additional, Iqbal, Rehana, additional, Jabeen, Farukh, additional, Jabeen, Mudassir, additional, Javed, Sadia, additional, Joyia, Faiz Ahmad, additional, Kamal, Shagufta, additional, Karadag, Ahmet, additional, Khaliq, Zubair, additional, Khera, Rasheed Ahmad, additional, Khosa, Muhammad Kaleem, additional, Lodhi, Ansab Zaeem, additional, Mahmood, Arslan, additional, Mahmood, Khalid, additional, Malik, Fozia Zahur, additional, Malik, Manzoor Ahmad, additional, Malik, Muhammad Imran, additional, Malik, Shoaib Ahmad, additional, Mansha, Asim, additional, Manzoor, Kaiser, additional, Mohammadi, Abbas, additional, Mohammadi, Mohsen, additional, Mousa, Mohanad, additional, Muha-ud-Din, Ruqaiza, additional, Murtaza, Mian Anjum, additional, Muzammil, Zaeema, additional, Najam-ul-Haq, Muhammad, additional, Naseer, Rehana, additional, Nasif, Muhammad, additional, Noreen, Aqdas, additional, Noreen, Razia, additional, Perveen, Shagufta, additional, Purwar, Roli, additional, Rashid, Umer, additional, Rasul, Azhar, additional, Rather, Sami ullah, additional, Raza, Syed Hammad, additional, Rehman, Att Ur, additional, Rehman, Fazal-ur, additional, Rehman, Saima, additional, Saeed, Muhammad Muzammal, additional, Salman, Mahwish, additional, Shafiq, Fahad, additional, Sobhani, Hadi, additional, Somi, Tahira, additional, Sultan, Neelum, additional, Sultana, Salma, additional, Sultana, Sitwat, additional, Sultana, Tayyaba, additional, Tabasum, Shazia, additional, Talukdar, Md Ikbal Ahmed, additional, Ul Hasan, Muhammad Sajjad, additional, Yaqoob, Nazia, additional, Younas, Mohammad, additional, Zafar, Sara, additional, Zahid, Muhammad, additional, Zahoor, Ameer Fawad, additional, Zhang, Baoliang, additional, Zia, Fatima, additional, Zia, Khalid Mahmood, additional, Zia, Muhammad Anjum, additional, and Zuber, Mohammad, additional

Published
2020
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21. Exploring the Green Power Unleashed by ZnO-Immobilized Aspergillus oryzaeCutinase for the Degradation of Insecticide and Polyester Waste

Author

Ali, Sikander, Qamar, Hira, Ahmad, Muhammad Usman, Saleem, Aroona, Ali, Baber, Aftab, Muhammad Nauman, Salmen, Saleh H., Ansari, Mohammad Javed, and Javed, Muhammad Ammar

Abstract

The present study deals with the enhanced production of cutinase (E.C. 3.1.1.74) by Aspergillus oryzaeISL-9 under submerged fermentation and its immobilization on ZnO nanocrystals for improved stability. The wild-type (ISL-9) was optimized for various parameters viz. substrate level (2%), sucrose concentration (1%), time of incubation (72 h), size of inoculum (3%), and pH (6). The activity was further enhanced by immobilization on ZnO nanocrystals. The UV–Vis spectrum showed an absorption band for both immobilized and free enzymes at 310 nm and 290 nm, respectively. The results of XRD showed the crystalline nature of ZnO nanocrystals. In FTIR, the observed bands from 1120.6 cm−1, 590.2 cm−1, 601.7 cm−1, and 1105.2 cm−1showed the stretching vibrations of nanocrystals of immobilized and free enzymes, respectively. The SEM images confirmed the particle size of the ZnO nanocrystals of 120–149 nm. Further, the polyester hydrolysis and insecticide degradation potential of the cutinase produced by wild-type ISL-9 was also evaluated. Under the optimized conditions of 0.6 ml of crude enzyme extract, 30 mg ZnO nanocrystals, and 30 min incubation, an overall 16.236% of polyester was hydrolyzed and 26.62% of insecticide was degraded by the enzyme. The immobilized enzyme showed a 1.19-fold and 1.35-fold increase in enzyme activity for polyester and insecticide hydrolysis, respectively, when compared with the free enzyme. The results are highly significant indicating the commercial availability of the process after the scale-up studies.

Published
2024
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24. Bsexlx of Engineered T. Reesei Strain as Dual-Active Expansin to Boost Cellulases Secretion for Synergistic Enhancement Of Biomass Enzymatic Saccharification in Corn and Miscanthus Straws

Author

Peng, Liangcai, primary, Xu, Chengbao, additional, Xia, Tao, additional, Peng, Hao, additional, Liu, Peng, additional, Wang, Yihong, additional, Wang, Yanting, additional, Kang, Heng, additional, Tang, Jingfeng, additional, and Aftab, Muhammad Nauman, additional

Published
2023
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25. Impact of Locally Characterized Protease from Geobacillus SBS 4S on the Growth of Poultry Bird

Author

Ahmad, Waheed, primary, Tayyab, Muhammad, additional, Muneer, Bushra, additional, Hashmi, Abu Saeed, additional, Ahmad, Mansurud Din, additional, Saeed, Shagufta, additional, Aftab, Muhammad Nauman, additional, Firyal, Sehrish, additional, Wasim, Muhammad, additional, Azam, Muhammad, additional, Talha, Muhammad, additional, and Awan, Ali Raza, additional

Published
2023
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37. Additional file 2 of Integrated NIRS and QTL assays reveal minor mannose and galactose as contrast lignocellulose factors for biomass enzymatic saccharification in rice

Author

Hu, Zhen, Wang, Youmei, Liu, Jingyuan, Li, Yuqi, Wang, Yanting, Huang, Jiangfeng, Ai, Yuanhang, Chen, Peng, He, Yuqing, Aftab, Muhammad Nauman, Wang, Lingqiang, and Peng, Liangcai

Subjects
technology, industry, and agriculture, food and beverages, macromolecular substances, complex mixtures
Abstract

Additional file 2: Figure S1. Applicability test of previously established NIRS modes [35]. A Spectrum variation range of prediction set and modeling set. B Distribution of prediction set and modeling set in principal components space of spectra. C–E Accuracy verification of NIRS models by comparing experimental and predicted values of 20 rice samples. ASL, acid soluble lignin; AIL, acid insoluble lignin; Glc-Rel1, glucose released after biomass enzymatic digestion following 1% (m/v) NaOH pretreatment. The fitting equations and coefficients are shown at the top of the plot. * and ** as significant correlations at P < 0.05 and 0.01, respectively. Figure S2. Frequency distributions of monosaccharides, acid soluble/insoluble lignin and biomass saccharification. A Mannose; B Galactose; C Acid soluble lignin; D Acid insoluble lignin; E Glucose yields released from enzymatic hydrolyses after 1% NaOH pretreatments (n = 215). Figure S3. Correlation analysis between lignin/cellulose related traits and enzymatic saccharification of alkaline pretreated rice straw. (A–G) Correlations of ρ-hydroxy-phenyl lignin H, syringyl lignin S, guaiacyl lignin G, acid soluble lignin (ASL), acid insoluble lignin (AIL), cellulose crystallinity index (CrI) and crystalline cellulose (Cry-cel) with glucose released after enzymatic digestion following 0.025% (m/v) NaOH pretreatment (Glc-Rel0.025). (H–N) Correlations of H, S, G, ASL, AIL, CrI and Cry-cel with glucose released after enzymatic digestion following 1% (m/v) NaOH pretreatment (Glc-Rel1). The fitting equations and coefficients are shown at the top of the plot. * and ** indicate the correlations are significant at p < 0.05 and 0.01, respectively. n = 100

Published
2021
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38. Additional file 1 of Integrated NIRS and QTL assays reveal minor mannose and galactose as contrast lignocellulose factors for biomass enzymatic saccharification in rice

Author

Hu, Zhen, Wang, Youmei, Liu, Jingyuan, Li, Yuqi, Wang, Yanting, Huang, Jiangfeng, Ai, Yuanhang, Chen, Peng, He, Yuqing, Aftab, Muhammad Nauman, Wang, Lingqiang, and Peng, Liangcai

Subjects
technology, industry, and agriculture, food and beverages
Abstract

Additional file 1: Table S1. Cellulose and lignin related traits of 100 rice straw samples. Table S2. Coefficient determination of calibration of 42 mannose and galactose NIRS models

Published
2021
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42. Integrated NIRS and QTL assays reveal minor mannose and galactose as contrast lignocellulose factors for biomass enzymatic saccharification in rice

Author

Hu, Zhen, primary, Wang, You Mei, additional, Liu, Jingyuan, additional, Li, Yuqi, additional, Wang, Yan Ting, additional, Huang, Jiangfeng, additional, Ai, Yuanhang, additional, Chen, Peng, additional, He, Yuqing, additional, Aftab, Muhammad Nauman, additional, Wang, Lingqiang, additional, and Peng, Liangcai, additional

Published
2021
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50. Biosynthesis and characterization of silver nanoparticles from Cedrela toonaleaf extracts: An exploration into their antibacterial, anticancer, and antioxidant potential

Author

Javed, Muhammad Ammar, Ali, Baber, Sarfraz, Muhammad Hassan, Ali, Sikander, Liaqat, Erum, Afzal, Muhammad Sohail, Wang, Yanting, Peng, Liangcai, ur Rehman, Asad, Aftab, Muhammad Nauman, Alarjani, Khaloud Mohammed, and Elshikh, Mohamed Soliman

Abstract

This research work aims to synthesize environmentally benign and cost-effective metal nanoparticles. In this current research scenario, the leaf extract of Cedrela toonawas used as a reducing agent to biosynthesize silver nanoparticles (AgNPs). The synthesis of AgNPs was confirmed by the color shift of the reaction mixture, i.e., silver nitrate and plant extract, from yellow to dark brown colloidal suspension and was established by UV-visible analysis showing a surface plasmon resonance band at 434 nm. Different experimental factors were optimized for the formation and stability of AgNPs, and the optimum conditions were found to be 1 mM AgNO3concentration, a 1:9 ratio of extract/precursor, and an incubation temperature of 70°C for 4 h. The Fourier transform infrared spectroscopy spectra indicated the presence of phytochemicals in the leaf extract that played the role of bioreducing agents in forming AgNPs. X-ray diffraction patterns confirmed the presence of AgNPs with a mean size of 25.9 nm. The size distribution and morphology of AgNPs were investigated by scanning electron microscopy, which clearly highlighted spherical nanoparticles with a size distribution of 22–30 nm with a mean average size of 25.5 nm. Moreover, prominent antibacterial activity was found against Enterococcus faecalis(21 ± 0.5 mm), Bacillus subtilis(20 ± 0.9 mm), Pseudomonas aeruginosa(18 ± 0.3 mm), Staphylococcus aureus(16 ± 0.7 mm), Klebsiella pneumoniae(16 ± 0.3 mm), and Escherichia coli(14 ± 0.7 mm). In addition, antioxidant activity was determined by DPPH and ABTS assays. Higher antioxidant activity was reported in AgNPs compared to the plant extract in both DPPH (IC50= 69.62 µg·ml−1) and ABTS assays (IC50= 47.90 µg·ml−1). Furthermore, cytotoxic activity was also investigated by the MTT assay against MCF-7 cells, and IC50was found to be 32.55 ± 0.05 µg·ml−1. The crux of this research is that AgNPs synthesized from the Cedrela toonaleaf extract could be employed as antibacterial, antioxidant, and anticancer agents for the treatment of bacterial, free radical-oriented, and cancerous diseases.

Published
2024
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