1. Rapid detection of African swine fever virus via SERS probe-modified sandwich hybridization assay.
- Author
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Wang Y, Yin H, Qi X, Wang C, Li B, Qian B, Zou M, and Xue F
- Subjects
- Animals, Biosensing Techniques methods, Swine, DNA, Viral analysis, DNA, Viral genetics, Limit of Detection, African Swine Fever diagnosis, African Swine Fever virology, African Swine Fever Virus isolation & purification, African Swine Fever Virus genetics, Spectrum Analysis, Raman methods, Nucleic Acid Hybridization, Metal Nanoparticles chemistry, Gold chemistry
- Abstract
Rapid and reliable detection method for African swine fever virus (ASFV) is proposed by surface-enhanced Raman spectroscopy (SERS). The ASFV target DNA can be specifically captured by sandwich hybridization between nanomagnetic beads and a SERS probe. Experimental results show that the significant Raman signal of the SERS probe with gold nanoparticles and a molecular reporter DTNB (5,5'-dimercapto-bis (2-nitrobenzoic acid)) can be adopted for detecting the hybridization chain reaction of ASFV DNA. The advantage of the SERS sandwich hybridization assay is the large response range from the single molecule level to 10
8 copies per mL, which not only can overcome the tedious time required for the amplification reaction but also provides a comparative method to polymerase chain reaction. Furthermore, real samples of African swine fever virus were detected from different subjects of swine fever virus including porcine reproductive respiratory syndrome virus and Japanese encephalitis virus. The proposed biosensor method can rapidly detect ASFV correctly within 15 min as a simple, convenient, low-cost detection approach. The biosensor can be used as a platform for the determination in biological, food, and environmental analytical fields., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)- Published
- 2024
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