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1. Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

Author

Vladimirov, Nikita, Voigt, Fabian F., Naert, Thomas, Araujo, Gabriela R., Cai, Ruiyao, Reuss, Anna Maria, Zhao, Shan, Schmid, Patricia, Hildebrand, Sven, Schaettin, Martina, Groos, Dominik, Mateos, José María, Bethge, Philipp, Yamamoto, Taiyo, Aerne, Valentino, Roebroeck, Alard, Ertürk, Ali, Aguzzi, Adriano, Ziegler, Urs, Stoeckli, Esther, Baudis, Laura, Lienkamp, Soeren S., and Helmchen, Fritjof

Published
2024
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2. Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

Author

Vladimirov, Nikita; https://orcid.org/0000-0002-1159-0689, Voigt, Fabian F; https://orcid.org/0000-0002-7108-2176, Naert, Thomas; https://orcid.org/0000-0003-3543-2519, Araujo, Gabriela R; https://orcid.org/0000-0003-4456-0774, Cai, Ruiyao; https://orcid.org/0000-0002-3050-8863, Reuss, Anna Maria; https://orcid.org/0000-0003-1096-3030, Zhao, Shan, Schmid, Patricia, Hildebrand, Sven; https://orcid.org/0000-0003-3783-8213, Schaettin, Martina; https://orcid.org/0000-0002-1487-6324, Groos, Dominik; https://orcid.org/0000-0002-1178-1354, Mateos, José María; https://orcid.org/0000-0002-3675-6198, Bethge, Philipp; https://orcid.org/0000-0003-4022-0844, Yamamoto, Taiyo; https://orcid.org/0000-0002-0305-8465, Aerne, Valentino, Roebroeck, Alard; https://orcid.org/0000-0003-0895-1145, Ertürk, Ali; https://orcid.org/0000-0001-5163-5100, Aguzzi, Adriano; https://orcid.org/0000-0002-0344-6708, Ziegler, Urs; https://orcid.org/0000-0001-7860-1867, Stoeckli, Esther; https://orcid.org/0000-0002-8485-0648, Baudis, Laura; https://orcid.org/0000-0003-4710-1768, Lienkamp, Soeren S; https://orcid.org/0000-0003-2963-6865, Helmchen, Fritjof; https://orcid.org/0000-0002-8867-9569, Vladimirov, Nikita; https://orcid.org/0000-0002-1159-0689, Voigt, Fabian F; https://orcid.org/0000-0002-7108-2176, Naert, Thomas; https://orcid.org/0000-0003-3543-2519, Araujo, Gabriela R; https://orcid.org/0000-0003-4456-0774, Cai, Ruiyao; https://orcid.org/0000-0002-3050-8863, Reuss, Anna Maria; https://orcid.org/0000-0003-1096-3030, Zhao, Shan, Schmid, Patricia, Hildebrand, Sven; https://orcid.org/0000-0003-3783-8213, Schaettin, Martina; https://orcid.org/0000-0002-1487-6324, Groos, Dominik; https://orcid.org/0000-0002-1178-1354, Mateos, José María; https://orcid.org/0000-0002-3675-6198, Bethge, Philipp; https://orcid.org/0000-0003-4022-0844, Yamamoto, Taiyo; https://orcid.org/0000-0002-0305-8465, Aerne, Valentino, Roebroeck, Alard; https://orcid.org/0000-0003-0895-1145, Ertürk, Ali; https://orcid.org/0000-0001-5163-5100, Aguzzi, Adriano; https://orcid.org/0000-0002-0344-6708, Ziegler, Urs; https://orcid.org/0000-0001-7860-1867, Stoeckli, Esther; https://orcid.org/0000-0002-8485-0648, Baudis, Laura; https://orcid.org/0000-0003-4710-1768, Lienkamp, Soeren S; https://orcid.org/0000-0003-2963-6865, and Helmchen, Fritjof; https://orcid.org/0000-0002-8867-9569

Abstract

In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging larger samples at higher speed and resolution has increased, requiring major improvements in the capabilities of such microscopes. Here, we introduce the next-generation mesoSPIM (“Benchtop”) with a significantly increased field of view, improved resolution, higher throughput, more affordable cost, and simpler assembly compared to the original version. We develop an optical method for testing detection objectives that enables us to select objectives optimal for light-sheet imaging with large-sensor cameras. The improved mesoSPIM achieves high spatial resolution (1.5 µm laterally, 3.3 µm axially) across the entire field of view, magnification up to 20×, and supports sample sizes ranging from sub-mm up to several centimeters while being compatible with multiple clearing techniques. The microscope serves a broad range of applications in neuroscience, developmental biology, pathology, and even physics.

Published
2024

3. The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples

Author

Vladimirov, Nikita, Voigt, Fabian F, Naert, Thomas, Araujo, Gabriela R, Cai, Ruiyao, Reuss, Anna Maria, Zhao, Shan, Schmid, Patricia, Hildebrand, Sven, Schaettin, Martina, Groos, Dominik, Mateos, José María, Bethge, Philipp, Yamamoto, Taiyo, Aerne, Valentino, Roebroeck, Alard, Ertürk, Ali, Aguzzi, Adriano, Ziegler, Urs, Stoeckli, Esther, Baudis, Laura, Lienkamp, Soeren S, Helmchen, Fritjof, and University of Zurich

Subjects
10017 Institute of Anatomy, 10242 Brain Research Institute, 530 Physics, U9 Adaptive Brain Circuits in Development and Learning (AdaBD), 10208 Institute of Neuropathology, 570 Life sciences, biology, 610 Medicine & health, 10192 Physics Institute, 10124 Institute of Molecular Life Sciences
Published
2023
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4. The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples.

Author

Vladimirov N, Voigt FF, Naert T, Araujo GR, Cai R, Reuss AM, Zhao S, Schmid P, Hildebrand S, Schaettin M, Groos D, Mateos JM, Bethge P, Yamamoto T, Aerne V, Roebroeck A, Ertürk A, Aguzzi A, Ziegler U, Stoeckli E, Baudis L, Lienkamp SS, and Helmchen F

Abstract

In 2015, we launched the mesoSPIM initiative (www.mesospim.org), an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging larger samples at higher speed and resolution has increased, requiring major improvements in the capabilities of light-sheet microscopy. Here, we introduce the next-generation mesoSPIM ("Benchtop") with significantly increased field of view, improved resolution, higher throughput, more affordable cost and simpler assembly compared to the original version. We developed a new method for testing objectives, enabling us to select detection objectives optimal for light-sheet imaging with large-sensor sCMOS cameras. The new mesoSPIM achieves high spatial resolution (1.5 μm laterally, 3.3 μm axially) across the entire field of view, a magnification up to 20x, and supports sample sizes ranging from sub-mm up to several centimetres, while being compatible with multiple clearing techniques. The new microscope serves a broad range of applications in neuroscience, developmental biology, and even physics.

Published
2023
Full Text
View/download PDF

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