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Energy restriction and manipulation of macronutrient composition of the diet are the main approaches that are used by people who aim to lose weight. When such strategies are employed, appetite and endocrine regulators of satiety, such as gut peptides, all are deeply affected. The gut microbiota-brain axis controls energy homeostasis in humans by affecting central satiety and gut peptides. The purpose of this study was to evaluate if the synergistic effect of probiotics and vitamin D in yogurt matrix can modulate this effect. In the double-blind, randomized, placebo-controlled trial, 140 obese adults were randomly allocated into four groups: 1) regular yogurt plus low-calorie diet; 2) PY plus low-calorie diet; 3) vitamin D-fortified yogurt plus low-calorie diet, and 4) probiotic and vitamin D co-fortified yogurt plus low-calorie diet. All groups were encouraged to increase their physical activity. Glucagon-like peptide-1 (GLP-1), peptide Tyrosin-Tysrosin (PYY), ghrelin, anthropometric variables, insulin, fasting blood sugar (FBS), insulin resistance/sensitivity, 1,25(OH)

Emerging technologies are being constantly improved, giving rise to new processes capable of promoting greater nutritional preservation and improved technological characteristics, in addition to the benefits related to energy efficiency. Ultrasound treatment has stood out among the non-thermal technologies, due to several benefits related to microstructural changes and microbial inactivation. The study of this technology is particularly important in dairy products, which represent one of the most important segments of the food industry and have a wide range of products developed from different processes. It has been shown that depending on the applied ultrasound treatment intensity (combination of frequency, power, processing time and sample volume) and the resulted temperature, the treatment may exert opposite effects on the food products, such as inactivation/stimulation of microorganisms and enzymes. Therefore, the purpose of this review was to contextualize the different applications and mechanism of action of ultrasound in milk and dairy products, approaching the technological benefits from the microbiological point of view.

Background Following the COVID-19 pandemic (caused by the SARS-CoV-2 coronavirus) at the beginning of 2020, containment measures have been taken by different countries around the globe. Citizens were forced to stay in quarantine, affecting their food consumption habits and food sector. These impacts have not yet been properly understood. Thus, it is important to describe the consequences of COVID-19 on food consumption habits globally, especially in the context of developing countries, such as Brazil. Scope and approach In this study, the Brazilian's food consumption habits and perceptions during the COVID-19 pandemic were assessed, highlighting the food consumption changes and selection of food products. Consumer perceptions about issues related to food safety and food marketing were also assessed. An online survey was performed and data were analyzed by descriptive analysis; independence and per cell chi-square test; and factor analysis. Key findings and conclusions Brazilians perceptions indicated that the COVID-19 pandemic context (assessed in May 2020) changed their food consumption and purchase. Respondents stated that they are eating and buying a greater amount of food, indicating a perception of a less healthy diet, mainly by women. On other hand, they are prioritizing homemade preparations and fresh food. Moreover, they reduced their shopping trips to markets and are starting to use delivery services and shopping platforms. Basic products of animal, vegetable, and bakery origin are being preferred during this period, in addition to economical packaging and products. Brazilians also indicated that they are more concerned with food safety and hygienic practices. However, at a time when global health is threatened, government agencies must create measures that ensure the food supply and consumer's awareness, in order to guarantee the country's food security during the current crisis.

The processing of high-protein vanilla-flavored milk was performed under different electric field strengths of ohmic heating (5.22 V/cm, OH6; 6.96 V/cm, OH8; 8.70 V/cm, OH10; 10.43 V/cm, OH12) to evaluate the energy consumption, processing parameters, and microbiological, rheological, and biological aspects, compared with the sample submitted to conventional pasteurization (PAST, 72 °C/15 s). All samples showed higher than 12 g/100 mL of protein, consisting of high-protein content products. In addition, Ohmic Heating (OH) generated lower energy expenditure and more significant microbial inactivation of lactic acid bacteria, molds and yeasts, total mesophiles, and psychotropics. Furthermore, OH at lower electric field strengths, mainly OH8, improved anti-diabetic, anti-oxidant, and anti-hypertensive activities and rheological properties, and resulted in lower hydroxymethylfurfural contents, and higher whey protein nitrogen index. The results suggest that OH is a technology that can be used in flavored milk with high-protein content, being recommended an electric field strength of 6.96 V/cm. However, more studies are necessary to evaluate the effect of OH on high-protein dairy products, mainly by studying other OH processing parameters.

Background Quantitative microbiological risk assessment (QMRA) is the most complex stage of risk analysis, an important management tool that considers the entire food production chain. Through safety limits, health authorities and producers are concerned with achieving the minimum risk to consumer health. Dairy products are a class of foods very susceptible to microbial contamination, and need an extensive database of studies in this regard. Scope and approach This review aims to provide an understanding of the structure of QMRA and its application in dairy products, taking into account the processing chains of dairy products and the influence of study factors in different regions. The limitations and factors of complexity in the construction of the QMRA models in milk and cheese pasteurized or not, are also presented. Key findings and conclusion Considerable differences were found in consumption habits between populations in different regions, significantly affecting the risk associated with infection or disease. Transport and retail conditions of the products also show a strong influence on the final risk, as well as the hygiene conditions of the milk used as raw material. Studies of this type are scarce, especially in dairy products, requiring further development of QMRA models. Risk assessment is a promising tool for verifying the effectiveness of using new processing technologies, based on the search for the lowest possible risk associated with food.

At present, there is an increasing demand for probiotic enriched plant-based milk products. Although many varieties of plant-based milk exist, soymilk, rice-milk and coconut-milk are the major carrier matrices used in probiotic food development. In general, these products are safe for consumption and can be considered as a suitable vehicle for delivering probiotics due to their ability in maintaining sufficient probiotic levels during product shelf life. However, studies that are focusing on the evaluation of functional efficacy of probiotics in these products with special reference to gastrointestinal survival, adhesion to intestinal epithelium and immunomodulation are scarce and these aspects need to be further assessed.

Background In the last years, consumers are more conscious about nutrition, welfare, authenticity, and quality of dairy products. Nuclear magnetic resonance (NMR) technology has gained attention as a potential method for food quality and authenticity purposes. Scope and approach This review aimed to comprehensively explain and discuss the use of NMR for quality and authenticity of milk and dairy products. Key findings and conclusions The NMR technology showed to be an effective tool to ensure the authenticity and to control the quality of dairy products during the processing and storage. It may be used to measure the fat content of milk and dairy products, evaluate the geographic origin, the molecular water mobility and the water retention capacity of cheeses, study the water mobility in milk beverages, and quantify the bound water molecules in ice creams. Additionally, NMR can detect whey, urea, hydrogen peroxide, synthetic urine, and synthetic milk in raw milk. Furthermore, it can evaluate physicochemical and sensory parameters of the products. NMR is a non-destructive technique, no need sample preparation or solvent separation, is fast and simple, and has high instrumental stability. However, it has disadvantages, like the high costs of acquisition and maintenance. The characterization of dairy foods may benefit from the combination of NMR and chemometrics, mainly to deal with large data matrices and highlight differences/similarities among samples.

The objective of this study was to assess the perceptions (using the preferred attribute elicitation [PAE] methodology) and acceptance of frozen dessert processed with water-soluble extract of rice byproduct and added with prebiotic components (long-chain inulin, medium-chain inulin, oligofructose, or polydextrose, 5 g/100 g) by vegan or nonvegan consumers. Most of the elicited attributes (9 out of 13 attributes, yellow color, brightness, creamy appearance, passion fruit aroma, sweet taste, passion fruit flavor, acid taste, sour taste, and creamy texture) were considered important for the characterization and/or acceptance of the frozen dessert formulations by both groups (vegan and nonvegan), but the order of importance was different between the groups. The sensory profile (Rv = 0.48, P = 0.03 in MFA) of the frozen dessert formulations was similar between vegan and nonvegan groups, and polydextrose contributed to increase firmness/consistency of the frozen dessert, while long-chain inulin contributed to the increase in the creaminess of the products. Finally, oligofructose and polydextrose could reduce the sour taste of the products. The consumers gave scores from 6 to 8 in a 9-point hedonic scale for the products, suggesting suitable acceptance. However, vegan consumers gave lower scores for the same products. In conclusion, PAE methodology can be used to compare the perception of different consumer groups, and vegan and nonvegan consumers have a similar perception about prebiotic frozen dessert processed with water-soluble extract of rice byproduct. Furthermore, the frozen dessert developed had suitable consumer acceptance, although vegan gave lower scores than nonvegan consumers. PRACTICAL APPLICATION: This is the first study involving the development of prebiotic frozen dessert from water-soluble extract of rice byproduct and application of PAE to assess the sensory perception of vegan and nonvegan consumers. The results are important for the industry as they indicate that the PAE methodology can be used to characterize food products, compare the perception of different consumer groups, and elicit attributes that are important for the products, suggesting that universal marketing strategies could be used and that the developed frozen dessert could serve both vegan consumers and those on conventional diets.

The trends related to ohmic heating technology in food processing were evaluated using bibliometric analysis based on the scientific literature published in the last decade. Publications from Turkey, Brazil, and Iran represent 32% of all publications. Most studies have targeted the definition of the best combinations of operational parameters for application in different food matrices and their possible effects on the food properties. In addition, a tendency to use ohmic heating as an alternative technology for pasteurization was observed. Future studies should develop mathematical models that evaluate process parameters and food characteristics in the inactivation of microorganisms and enzymes and maintenance of bioactive compounds, the study of the non-thermal effect of electromagnetic waves on the food quality, the evaluation of the processing conditions and food physicochemical properties in the electrode corrosion and migration of metal ions to the treated food, and improvements of homogeneity during processing. This study was the first to perform a bibliometric analysis based on scientific literature concerning ohmic heating in food processing and presented the challenges, future trends, and evolution of scientific research.

This review was the first to gather literature about the effect of emerging technologies on probiotic, prebiotic, and postbiotic products. Applying emerging technologies to probiotic products can increase probiotic survival and improve probiotic properties (cholesterol attachment, adhesion to Caco-2 cells, increase angiotensin-converting enzyme (ACE) inhibitory, antioxidant, and antimicrobial activities, and decrease systolic blood pressure). Furthermore, it can optimize the fermentation process, produce or maintain compounds of interest (bacteriocin, oligosaccharides, peptides, phenolic compounds, flavonoids), improve bioactivity (vitamin, aglycones, calcium), and sensory characteristics. Applying emerging technologies to prebiotic products did not result in prebiotic degradation. Still, it contributed to higher concentrations of bioactive compounds (citric and ascorbic acids, anthocyanin, polyphenols, flavonoids) and health properties (antioxidant activity and inhibition of ACE, α-amylase, and α-glucosidase). Emerging technologies may also be applied to obtain postbiotics with increased health effects. In this way, current studies suggest that emerging food processing technologies enhance the efficiency of probiotics and prebiotics in food. The information provided may help food industries to choose a more suitable technology to process their products and provide a basis for the most used process parameters. Furthermore, the current gaps are discussed. Emerging technologies may be used to process food products resulting in increased probiotic functionality, prebiotic stability, and higher concentrations of bioactive compounds. In addition, they can be used to obtain postbiotic products with improved health effects compared to the conventional heat treatment.

Made available in DSpace on 2022-04-28T19:50:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 This study aimed to assess the energy consumption of ohmic heating (OH) and conventional heating (CH) for pasteurization of fresh and thawed sheep milk and their impact on bacterial microbiota throughout refrigerated shelf-life (4 °C ± 1, 15 days). OH pasteurization using 8.33 and 5.83 V/cm electric field strength spent 72–73% less energy than CH pasteurization (515 KJ). The cultivation-dependent approach showed that at least 4.2 log cycle reductions were achieved in sheep milk submitted to CH and OH pasteurization, regardless sheep milk was fresh or thawed. Data from amplicon sequencing indicated that Staphylococcus was the most prevalent genus in raw samples at day 1 (F1D1: 58.35%; T1D1: 69.50%), while Pseudomonas became the most abundant after 15 days under cold storage (F1D15: 50.15% and T1D15: 54.50%). The relative abundance of all bacterial genera assessed remained similar on pasteurized samples by CH and OH throughout refrigerated storage. Industrial relevance: Ohmic heating (OH) presents as a critical advantage rapid and uniform heating of fluid food material. No studies assessed the use of OH for pasteurization of sheep milk and to evaluate the impact of this technology on sheep milk bacterial microbiota during refrigerated storage. The findings of this study prove the feasibility of sheep milk pasteurization using OH8.33 V/cm. OH also ensured the bacteriological stability of sheep milk during 2 weeks of storage under refrigeration conditions prior to dairy products manufacturing. This approach comprises a cheaper and easier way to store milk when compared to frozen storage, with potential benefits to small farmers and dairy industries. Department of Food Science and Nutrition Faculty of Food Engineering University of Campinas Institute of Biosciences Department of General and Applied Biology São Paulo State University Department of Veterinary Hygiene and Technological Processing of Animal Products Veterinary School Federal Fluminense University Research Informatics Core Research Resource Center University of Illinois at Chicago Department of Food Technology Federal Institute Goiano Department of Food Technology Federal Institute of Rio de Janeiro Institute of Biosciences Department of General and Applied Biology São Paulo State University

The effect of ohmic heating (OH) parameters (voltage gradient, 2, 4, 6, 8, and 10 V cm−1) on the quality properties of dulce de leche (DL) was evaluated. Optical (L*, C*, and BI), rheological (flow curves and oscillatory tests) and thermal (differential scanning calorimetry) parameters as well as microstructure (optical microscopy) and modeling of the crystal size growth were evaluated during the storage time (60 days at 25 °C). Overall, OH processing resulted in lower optical (C* and BI) parameters, indicating a product with a lower intensity of caramel brown color. For the rheological assays, it was observed a decrease in the viscosity and consistency of the products (lower k values and higher n and G’ values). Finally, the OH-treated products presented higher numbers of lactose crystals of smaller size. The impact of OH was dependent on the voltage gradient, with a more pronounced effect on the product submitted to high electric field (10 V cm−1). Overall, OH is an interesting technology to be used in DL processing, being recommended the utilization of 8 V cm−1 of voltage gradient due to the better-quality properties.

This study aimed to evaluate the perception of Brazilian dairy processors (n = 31) concerning food defense. The results showed that respondents consider the implementation of control procedures related to facilities, products, materials, and individuals as important measures in food defense. The higher agreement rates (strongly agreed + slightly agreed) of the companies in relation to the perception of food defense were 84% for external security, followed by personnel security (82%), generalities (81%), and internal security (74%). Thus, protecting facilities and controlling the traffic flow were considered to be the most important actions under the participants' perspectives. Employee satisfaction and identification of end products and raw materials are also considered relevant in the food defense program. Although food defense is not a formal requirement in Brazilian law, the results show that there is adequate awareness of this topic by the Brazilian dairy companies.

Background Thermal processing is the most common decontamination method used in the dairy industry to ensure food safety and extend shelf life. However, due to the increased consumer demand for more natural and healthier products, nonthermal technologies have been intensively studied. Irradiation with shortwave ultraviolet light (UV-C) offers some technological advantages due to its low maintenance and installation costs, minimal energy use, and food preservation without some undesirable effects of heat treatments. Scope and approach: This review aims to describe the theoretical fundamentals of UV-C radiation and approaching the critical processing factors involved. Also, it describes the mechanisms of microbial inactivation and provides an overview of the effects on dairy product quality, considering microbiological, physicochemical and sensory aspects. Key findings and conclusions UV-C radiation can be considered an effective method for inactivating pathogenic and spoilage microorganisms in milk and dairy products by forming lesions in DNA and/or damage in the cellular enzyme activity and cytoplasmatic membrane integrity. The efficiency of the process is dependent on the process parameters (time of exposure, UV dose, wavelength and UV light source), product type (chemical composition, viscosity, turbidity, opacity and roughness), equipment (conformation and geometry), and microorganism characteristics (specie, strain, initial count, growth phase, and recovery conditions). When optimum conditions are applied, there is no impact on the physicochemical, nutritional and sensory aspects. In conclusion, UV-C can be considered an environmental-friendly emergent non-thermal technology for decontamination of dairy products, presenting low costs and efficiency in the maintenance of the quality parameters of dairy products.

Background Postprandial glycemia after food intake is considered the most important parameter to control the risk of diabetes. Although probiotic dairy products may have a regulatory effect on postprandial blood glucose, this topic has been poorly addressed in scientific studies. Scope and approach This review aims to discuss the relationship between the consumption of probiotic dairy products and the maintenance of postprandial glycemia, presenting the mechanisms of action of probiotics and the impact of the dairy matrix, the probiotic strain, and addition of ingredients, among others. A comparison between the effects of dairy products and the synthetic drugs conventionally used was also carried out. Finally, the review deals with strategies for increasing the functionality of probiotic dairy products. Key findings and conclusion Ripened cheeses, fermented dairy products, and whey-based products (milk beverages) containing probiotic strains with higher proteolytic and EPS-forming capacities, and the addition of prebiotics and/or plant-derived products have a greater effect on postprandial glycemic regulation and/or inhibition of α-glucosidase and α-amylase enzymes. The literature has focused on the in vitro inhibitory effect on digestive enzymes, suggesting that clinical studies should be a priority. The inhibitory effect observed for the probiotic dairy products can be quantitatively comparable to that provided by synthetic drugs, depending on the dairy matrix and the probiotic strain, with promising results.

Functional food is the trend of the future, since our current science allows the manufacturing of processed products with health benefits beyond that of the original food, which meets the demand of the modern consumers. However, many functional ingredients may affect the products characteristics, including the texture, which is very important to the consumer’s acceptance. Probiotics (Lactobacillus spp., Bifidobacterium spp., etc.) and prebiotics (inulin, fructooligosaccharides, Galactooligosaccharides, etc.) are widely studied functional ingredients and may change the texture of the food, improving it or not. Studies are then focusing on developing functional products with improved textural characteristics associating the healthy feature with the sensory one, thus, adding great value for these products.

In the last few years, the interest of consumers for healthy products has increased, and the high capacity of probiotics to provide beneficial health effects led to the growing scientific and commercial interests facing the microbial administration as a health promoting strategy. Since the first definition of what is probiotics, the researches about functional foods advanced significantly, at the point of arising new emerging concepts, as paraprobiotics, postbiotics and psychobiotics, to refer to the non-viable microorganisms or metabolites able to provide physiological health benefits to the consumers, or to indicate the therapeutic action related to probiotics. Therefore, in this review those concepts were approached, as well as the potential applications in dairy products, highlighting the main technological advantages compared to the use of probiotics.

According to the proposed definition by FAO/WHO, probiotics must be alive and abundant once ingested. However, during recent years, new definitions are added to the probiotic terminology such as ‘paraprobiotics’ (dead/inactive cells of probiotics) and ‘postbiotics’ (healthful metabolites of probiotics), because findings have shown that dead cells (intact or ruptured) could also show significant health impacts on human. However, mentioned terms are not accurate and impressive enough to reflect the intended meanings regarding all states of probiotic benefits and it seems that a disturbance and confusion in probiotic glossary has been occurred. As a result, a new terminology based on a new efficient approach and conceptualization is needed for a global agreement and usage. In the present paper, we are honored to propose such terminology to provide mentioned comprehensive approach and terminology for all aspects of probiotic benefits. We are proposing three main classes of probiotic including ‘true probiotic’ (TP) referring to viable and active probiotic cell, ‘pseudo-probiotic’ (PP) referring to viable and inactive cell, in the forms of vegetative or spore (PPV or PPS) and ‘ghost probiotic’ (GP) referring to dead/nonviable cell, in the forms of intact or ruptured (GPI or GPR). Each of these classes are classified into two groups based on their site of action/impact: internal (in vivo) or external (in vitro).

The objective of this study was to evaluate the effect of environmental factors on the fatty acid profiles and physicochemical composition of Crassostrea gasar cultured in two locations with differences in salinity and precipitation gradients. The study period was divided into four seasonal divisions—the rainy–dry transition period, the dry season, dry–rainy transition and the rainy season. The salinity, pH and temperature were measured at each site during specimen collection. A total of 712 oyster samples were used for fatty acid profiling and physicochemical analysis. Salinity and precipitation were the environmental parameters that influenced the fatty acid profile and physicochemical characteristics of oysters. C. gasar exhibited excellent values of n3 polyunsaturated fatty acids (DHA, EPA and eicosatrienoic), with a predominance of these acids during periods of higher precipitation. Variations in precipitation and salinity resulted in significant differences in oyster nutritional characteristics. Oysters grown in Amazonian estuaries can be considered nutritious throughout the year, with better conditions during the rainy season.

The aim of this study was to investigate the effects of coffee species, roast degree and decaffeination on in vitro probiotic bacterial growth, and to identify the major coffee compounds responsible for such effects. Six C. arabica and C. canephora extracts (regular medium and dark roasted and decaffeinated medium roasted), and five bioactive compounds (chlorogenic acid, galactomannan, type 2 arabinogalactan, caffeine and trigonelline) were individually incorporated into a modified low-carbon broth medium-(mMRS), at different concentrations (0.5 to 1.5% soluble coffee and 0.05 to 0.8 mg mL-1 standard solutions). Inulin and fructooligosaccharides (FOS) were used as prebiotic references. MRS and mMRS were used as rich and poor medium controls, respectively. The growth of Lactobacillus rhamnosus GG ATCC 53103-(GG), L. acidophilus LA-5-(LA), Bifidobacterium animalis DN-173010-(BA) and B. animalis subsp. lactis BB12-(BB12), as well as the growth inhibition of non-probiotic Escherichia coli ATCC 25922 were evaluated. Differences in growth between mMRS and treatments (Δlog CFU mL-1) were compared by ANOVA and Tukey's test, and considered when p ≤ 0.05. Overall, after 48 h incubation, the medium roasted arabica coffee extract increased the growth of GG, LA and BA (range: Δlog CFU mL-1 = 0.5 to 1.8), while the dark roasted arabica coffee extract increased BB12 growth (range: Δlog CFU mL-1 = 0.9 to 1.7), in a dose dependent manner. Improved performances of GG, LA and BA were promoted by higher polysaccharides and CGA concentrations, with better performance for Lactobacillus sp. The tested coffee bioactive compounds promoted the poor growth of BB12. Plain caffeine did not promote Bifidobacterium sp. growth and limited the growth of Lactobacillus sp. Regular C. arabica and C. canephora extracts inhibited the growth of E. coli, while the decaffeinated extracts promoted its growth. The present results show that coffee consumption can selectively improve the growth of probiotic strains, thus exerting a prebiotic effect, and show that coffee roasting and decaffeination affect this property and that different strains utilize different coffee components to grow.

Background Foodborne diseases can be highlighted as one of the most significant health concerns among the last decades. Probiotic food products can be considered as the promising approaches for modulating of gastrointestinal (GIT) microbiota due to their interactions within the GIT. However, no comprehensive review regarding the involved mechanisms in inhibiting foodborne pathogens in foods by probiotics, besides their interaction is available. Scope and approach The current article provides an overview considering the interactions between probiotics and pathogens in hosts as well as in foods aiming to gain insights regarding relevant properties to be used in further developments of probiotic-based food products. Key findings and conclusions The interaction between probiotics and pathogens in foods and in the hosts and different mechanism of probiotics in control of enteric pathogens colonization were reviewed in the current study. While the mechanisms of action correlated with probiotic strains in the GIT are diverse and well-studied, their interactions with pathogens in foods is overlooked. Revealing how probiotic strains interact with foodborne pathogens in foods is of key relevance in a contemporary context that demand the development of more robust formulations. Although several mechanisms such as production of substances such as organic acids, bacteriocins, and hydrogen peroxide have been suggested regarding probiotics actions in food matrices, still substantial challenges exist concerning the molecular mode of their antimicrobial action. Additionally, it is required to comprehend the appreciate dose, species, and a combination of probiotics in controlling the pathogens.

Made available in DSpace on 2022-04-28T19:45:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) This study aimed to evaluate the effects of conventional heat treatments (high-temperature–short-time and low-temperature–low-time) and high-intensity ultrasound (HIUS) on the physical stability and bacterial diversity of whole milk during refrigerated storage. HIUS processing used an energy density of 3 kJ/mL and an ultrasound intensity of 90 W/cm2. The most abundant bacterial genera found in raw milk were Acinetobacter and Pseudomonas. After processing and storage for 7 days, Acinetobacter, Kluyvera and Serratia were the dominant genera in almost all samples, and Pseudomonas was the most abundant in HIUS-processed milk. Only the HIUS process reduced the fat droplet size (