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Background and Aim: Urethral obstruction (UO) is a common condition in feline medicine. Severe acid-base and electrolyte disorders promote relevant electrocardiographic changes in these animals. Cardiac biomarkers such as cardiac troponin I have been shown to be useful in identifying cats with myocardial disease, but it has not been investigated whether UO leads to myocardial damages. This study aimed to evaluate biochemical changes, electrocardiographic findings, troponin I measurements, and electrolyte disturbances for 7 days in cats with UO. Materials and Methods: This follow-up prospective study included 33 cats diagnosed with UO for 7 days. For all cats, clinical examination, serum biochemistry, electrolyte analyses, blood pressure, and electrocardiography were performed. Cardiac troponin I was measured in the serum in 16 cats at 3 different times. Results: The mean age of the feline population was 1.83±1.58 years (mean±standard deviation). Creatinine, urea, blood urea nitrogen, glucose, phosphorus, base excess, bicarbonate, and serum potassium decreased significantly (p≤0.05), while ionic calcium and blood pH increased significantly (p≤0.05) at different times. Electrocardiographic abnormalities were observed in 21/33 (63.63%) of the felines on admission day. The electrocardiographic abnormalities were no longer observed on the subsequent days. Only one feline showed changes in troponin I cardiac concentrations. Conclusion: This study suggests the sum and severity of electrolyte abnormalities aggravate the clinical and cardiovascular status of these patients. However, cTnI, blood pressure, and heart rate within the reference range do not exclude the presence of major cardiovascular and metabolic abnormalities. The hyperglycemia in felines with UO appears to be associated with decreased renal clearance, which may reflect the severity of hyperkalemia and azotemia. The metabolic and cardiovascular changes of these felines are minimized by the establishment of appropriate intensive care; however, cardiac and blood gas monitoring is essential to assess the severity of the disease.

Canine morbillivirus, also known as canine distemper virus (CDV), induces a contagious multisystemic disease caused by an enveloped RNA virus belonging to the genus Morbillivirus within the family Paramyxoviridae. CDV replicates readily in epithelial, nerve and lymphoid tissues; it is excreted in urine, feces, saliva, oral and nasal discharge; and its major route of entry for infection is through the respiratory system. Although the virus was originally believed to infect domestic dogs, new studies have shown that it can also naturally or experimentally infect non‐domestic hosts. A recent blood test performed on a giant anteater (Myrmecophaga tridactyla) found Lentz inclusions in the animal's leucocytes. A rapid CDV test, an RT‐PCR assay and pathology findings confirmed this report of canine morbillivirus in this species, which corresponds to the second report of CDV infection in the order Pilosa, family Myrmecophagidae in central west Brazil., This article is the first report of canine morbillivirus infection in a Giant anteater (Myrmecophaga tridactyla).The disease was confirmed through samples collected from the animal after the visualization of Lents corpuscles inside leucocytes during blood count cells.

Background and Aim: Canine visceral leishmaniasis (CanL) has a broad spectrum of changes, with kidney disease being considered the main cause of mortality. Thus, this study aimed to monitor serum and urinary biomarkers in response to two short-term treatments for CanL. Materials and Methods: Thirty dogs with CanL were equally divided into two treatment groups and treated with either miltefosine (Group M) or miltefosine plus allopurinol (Group MA); the groups were evaluated before treatment and after 28 days of treatment. Physical exams were performed and hematimetric, biochemical, and urinary parameters, including urinary biomarkers cystatin C (CisC), lipocalin-2 (NGAL), and microalbuminuria, were measured. Results: Both treatments significantly reduced clinical scores (p

A Erliquiose monocitica canina, causada pela bacteria intracelular Ehrlichia canis, pode acometer diferentes orgaos inclusive os rins, nas distintas fases da infeccao, sendo considerada uma das principais causas de obito relacionadas a essa doenca. Este trabalho teve por objetivo investigar a ocorrencia de doenca renal em caes naturalmente infectados por E. canis correlacionando a gravidade da doenca renal. Foram avaliados 79 caes para infeccao por E. canis pela reacao em cadeia pela polimerase e sorologia (dot ELISA), e a deteccao de doenca renal pela concentracao serica de ureia, creatinina, urinalise, gamaglutamil transferase urinaria e proteinuria e a gravidade da doenca renal pela classificacao da Sociedade Internacional de Interesse Renal. Dos caes pesquisados, 60 (75,9%) foram positivos na nested PCR e 70 (88,6%) apresentaram anticorpos anti- E. canis. Foram observadas alteracoes nos testes laboratoriais condizentes com doenca renal nos caes infectados, porem sem diferenca estatisticamente significativa (p>0,05). De acordo com a Sociedade internacional de Interesse Renal, 43,3% dos caes positivos encontravam-se no estagio I da doenca renal, apesar nao estatisticamente significativo, em 10% dos caes positivos a azotemia foi considerada de moderada a grave indicando intenso comprometimento renal. O encontro de uma estimulacao imunologica e de comprometimento renal na infeccao por E. canis, reforcam a necessidade de monitoramento da funcao renal desses animais.

Background: Blood transfusion is a tool capable of saving lives. Patients undergoing blood transfusion usually present several alterations in the acid-base and electrolyte balance, aggravating the condition of critically ill patients. Some studies have demonstrated haematological alterations in certain species that received whole blood transfusions, however, few studies have evaluated acid base and electrolyte changes in dogs undergoing whole blood haemotherapy. The aim of this study was to analyze clinical, hematological, blood gas and electrolyte changes in anemic dogs after whole blood transfusion.Materials, Methods & Results:Twenty nine dogs transfused due to anemia were enrolled in the study. Donors blood was collected in a transfusion bag containing citrate phosphate adenine dextrose and stored up to 24 h. Blood collections and evaluations were made before and 24 h after the transfusion. Data distribution normality was tested by the Shapiro Wilk Test. The means of the variables were compared by paired t-test. It was observed an increase in diastolic blood pressure and a reduction in heart rate (P < 0.05). There was a not significant increase in systolic blood pressure, temperature, and a reduction in respiratory rate per minute. Erythrocyte, haemoglobin and haematocrit averages were significantly increased after blood transfusion (P < 0.05). It was observed a reduction in the mean values of pH (P < 0.05), potassium (P > 0.05) and ionized calcium (P > 0.05) and an increase in the mean partial pressure of carbon dioxide (pCO2) (P < 0.001), bicarbonate (P > 0.05) and sodium (P < 0.05). The probable cause of anemia was monocytic ehrlichiosis (14/29), visceral leishmaniasis (1/29), babesiosis (1/29), co-infection of Ehrlichia canis and Leishmaniainfantum (2/19), co-infection of E. canis and Babesia vogeli (1/19). It was not possible to determine the etiology of the anemia in ten dogs. Discussion:Heart rate significantly reduced after transfusion, probably because of the increase in hematocrit, hemoglobin and erythrocyte values. It may be justified by the displacement of extravascular fluid to the intravascular space. Mean values of systolic blood pressure were slightly elevated before transfusion and remained elevated afterwards, while diastolic and mean arterial pressure increased significantly after transfusion. These changes may be due to the morbid condition and may be influenced by many other factors. Haematocrit, haemoglobin and erythrocyte values increased significantly after transfusion, according to what was observed in other studies. The significant reduction in pH and increase in pCO2 reflects the compensatory mechanism for metabolic acidosis to increase ventilation, leading to pCO2 reduction and changes in pH. The reduction in pH due to the contact of the collected blood with conservative solutions is one of the main changes thar occurs during blood storage. It was described significantly lower pH in dogs’ whole blood samples stored for more than 24h in vacutainer plastic containing CPDA-1. We may assume there was no intense pH reduction in the present study because the bags were stored for up to 24 h. Although not statistically significant, the increase of pO2 mean reflects the improvement of tissue oxygen perfusion. It was observed a significant increase in sodium ions. The mean sodium ionconcentration before transfusion was very close to the maximum reference value. Hyperkalaemia was not observed, nor was there significant reduction of potassium ions after transfusion. Several studies report hyperkalaemia and transfusion-associated cardiac arrests in humans, associated with infusion of large volumes of blood. Whole blood transfusion increased erythrogram values and did not negatively affect the electrolyte or acid-base status, representing a safe and useful tool in the intensive care of small animals.

As queimadas que ocorreram no ano de 2020 no Pantanal trouxeram intensos prejuízos à fauna e flora da região, sendo que o tamanduá-bandeira foi um dos mamíferos mais afetados pelas queimadas. O presente trabalho teve como objetivo descrever alterações hematológicas encontradas nos exames laboratoriais de um tamanduá-bandeira (Myrmecophaga tridactyla), vítima dos incêndios ocorridos no Pantanal mato-grossense, correlacionando-as ao quadro clínico do animal. O paciente foi resgatado no mês de setembro de 2020 com lesões graves por queimaduras nas regiões de membros e face. Após quatro dias de internação (D4) foi feita colheita de sangue para a realização de hemograma e bioquímica sérica. Notou-se diminuição nos valores de eritrócitos (0,98 x 106/mm3; 2,05 – 2,67 x106/mm3), hemoglobina (4,8 g/dl; 10,6 – 12,9 g/dl) e hematócrito (16%; 35,44 – 40,1%), contagem total de leucócitos dentro dos valores de normalidade (16,0 x 103/mm3; 5,59 – 18,14 x 103/mm3), com desvio à esquerda degenerativo, presença de metamielócitos (0,7 x 103/mm3; 0 – 0 x 103/mm3), bastonetes (5,2 x 103/mm3; 0 – 0,06 x 103/mm3) e segmentados (5,1 x 103/mm3; 3,91 – 3,88 x 103/mm3). Os eosinófilos (3,4 x 103/mm3; 0,08 – 1,86 x 103/mm3), monócitos (0,5 x 103/mm3; 0,01 – 0,2 x 103/mm3) e plaquetas (168 x 103/mm3; 52,2 – 134,4 x 103/mm3) estavam aumentados e os valores de proteínas plasmáticas totais diminuídos (4,2 g/dl; 7,77 – 8,42 g/dl). Os valores de ALT (121 UI/L; 7,51 – 23,47 UI/L), AST (143 UI/L; 13,62 – 28,62 UI/L) e CK (13252,0 UI/L; 41,45 – 81,77 UI/L), mostraram-se significativamente aumentados e de proteínas totais diminuído (4,8 g/dl; 5,49 – 6,61 g/dl). Queimaduras de segundo e terceiro graus em tamanduá-bandeira podem causar desvio à esquerda degenerativo, anemia intensa, hipoproteinemia e alterações marcantes de enzimas que avaliam dano muscular. O impacto das queimadas no Pantanal mato-grossense no ano de 2020 precisa ser avaliado, sendo necessária a implantação de políticas públicas de combate aos incêndios mais eficazes.

Introduction: Sepsis is a serious problem in felines with a mortality rate ranging from 29-79%. Neisseria spp. is considered a commensal microorganism of the oral cavity of dogs and cats and is usually isolated from human wounds resulting from bites of these animals. Case Report: The present report describes clinical, imaging and laboratory findings of a feline with sepsis wherein commensal and multidrug-resistant (MDR) Neisseria spp. was isolated. The feline presented a history of four days of anorexia, dyspnea, prostration, and, pericardial, pleural and abdominal effusions. Pericardiocentesis was performed and hemorrhagic exudate was observed. The animal died after 11 days of treatment with gentamicin and amoxicillin combined with clavulanic acid. During necropsy, the abdominal cavity was found to be filled with greenish-yellow content and the pericardial sac was thickened with a large amount of purulent secretion. Histopathology revealed sepsis with necrotizing suppurative pericarditis, diffuse mononuclear pneumonia and necrotic pleuritis, leading to secondary bacterial infection. Conclusions: Commensal Neisseria spp. are important zoonotic bacteria, which trigger a serious disease in felines. However, it has not been reported to cause sepsis with pneumonia, suppurative necrotizing pericarditis and pericardial effusion.

Visceral leishmaniasis (VL) is an infectious disease caused by the protozoan parasite Leishmania (Leishmania) infantum, an intracytoplasmic parasite that affects humans and other species of domestic and wild mammals. In Brazil, the treatment of canine visceral leishmaniasis (CVL) with miltefosine has been implemented since 2016, and the reports on the clinical and immunological conditions of treated dogs are scarce. Thus, this study aimed to assess and monitor the clinical, laboratory, and immunological condition of dogs with CVL before (D0) and after (D29) using three pharmacotherapeutic protocols: miltefosine monotherapy (Milteforan™, Virbac) (G1), miltefosine plus allopurinol (G2), and allopurinol monotherapy (G3). Forty-five dogs with CVL were assigned to one of three treatment groups. The dogs were evaluated for clinical signs, was well as haematological, biochemical, serological, and cytokine levels. Significant reduction in clinical scores was observed in all protocols, with no differences between groups. We did not observe a clinical cure in any of the dogs in the groups. Haematological and biochemical parameters showed slow recovery, with better results observed in G2. Anti-Leishmania antibody titre remained increased in all groups. The quantification of serum cytokines demonstrated a mixed Th1/Th2 profile in CVL. The IL-2 levels decreased in all groups after treatment. Evaluation of IFN-y and IL-10 did not show changes in the groups analysed, and it did not contribute to short term therapeutic monitoring. All therapeutic protocols promoted, to varying degrees, an improvement in the general condition (clinical signs, haematological, and biochemical levels) of the animals. Through clinical-pathological exams, we found that the combination of miltefosine plus allopurinol promoted better effects in the short-term, representing the best choice for the treatment of CVL, even when compared to the only therapeutic protocol allowed in Brazil, miltefosine monotherapy. Through the quantification of cytokines, IL-2 proved to be a potential therapeutic marker for the monitoring and follow-up of dogs with CVL.

Incêndios florestais ocorrem anualmente no Pantanal Mato-grossense nos períodos de seca causando morbidade e mortalidade de inúmeras espécies animais, onde os tamanduás-bandeira enquadram-se entre as principais vítimas dessas catástrofes. Devido a difícil criação dessa espécie em cativeiro, há poucos dados na literatura veterinária a respeito dos perfis hematológicos e bioquímicos de tamanduás-bandeira sadios e não há descrição sobre as alterações encontradas nesses animais com lesões térmicas ocasionadas pelo fogo. Portanto, objetivou-se a avaliação dos perfis hematológico e bioquímico de tamanduás-bandeira provenientes de áreas incendiadas do Pantanal (MT) no ano de 2020, assim como a determinação das alterações laboratoriais mais frequentes. No Laboratório de Patologia Clínica Veterinária do HOVET-UFMT foram realizados hemograma completo e bioquímica sérica de amostras sanguíneas de seis indivíduos da espécie Myrmecophaga tridactyla após admissão hospitalar. Dentre os resultados avaliados, observou-se que: 83,3% (5/6) dos animais apresentaram anemia normocítica hipocrômica nos eritrogramas, nos leucogramas 66,7% (4/6) apresentaram desvio à esquerda e neutropenia, além de outros achados predominantes nos hemogramas como hipoproteinemia plasmática total observada em 100% (6/6) dos indivíduos e trombocitose em 66,7% (4/6). Poucas alterações foram observadas na bioquímica sérica, diferentemente dos dados encontrados na literatura humana. Concluiu-se que tamanduás-bandeira com queimaduras térmicas graves apresentam principalmente alterações laboratoriais no hemograma e poucas alterações bioquímicas na primeira avaliação pós- resgate. Salientando assim a necessidade da realização destes exames em vários intervalos de tempo pós lesão para melhor compreensão das alterações laboratoriais sofridas por esses pacientes, haja vista, que todos os indivíduos desse estudo vieram a óbito.

RESUMO Nocardiose é causada por bactérias do gênero Nocardia do subgrupo Actinomycetos, que são Gram-positivas aeróbicas, filamentosas e podem apresentar ramificações. O diagnóstico baseia-se na presença de lesão inflamatória, com o microrganismo morfologicamente compatível, associada ao isolamento e à identificação microbiológica e molecular. Este trabalho tem por objetivo relatar um caso de nocardiose em canino, que desenvolveu inflamação piogranulomatosa peritoneal seis meses após ovariossalpingo-histerectomia. O animal apresentava hipertermia, distensão abdominal, taquipneia, polidipsia, hiporexia, mucosas hipocoradas e fezes pastosas. Os achados laboratoriais evidenciaram anemia leve e leucocitose por neutrofilia com desvio à esquerda e hipoalbuminemia. Uma massa na região mesogástrica e efusão peritoneal foram evidenciadas por meio da ultrassonografia abdominal. O líquido foi classificado como exsudato piogranulomatoso, e o animal submetido à laparotomia exploratória para lavagem abdominal e remoção da massa. Após procedimentos terapêuticos, ocorreu piora clínica e óbito. Peritonite piogranulomatosa foi a principal alteração anatomopatológica a qual foi associada à Nocardia spp. Molecularmente, a espécie isolada se aproxima da N. concava, por meio da análise filogenética. Essa espécie já foi descrita como causa de infecção em humanos na Ásia, no entanto não há registros na literatura na espécie canina, sendo este o primeiro relato.

Thrombocytosis refers to the increase in number of platelets per microliter (µL) of blood. Platelet counts greater than 1,000,000/µL may be associated with clinical signs of bleeding or thrombosis. Previous studies on underlying causes of thrombocytosis have aroused the interest of researchers about its clinical importance in dogs. The objective of this study was to analyze the blood cell counts in dogs in order to define the main diseases or clinical conditions that were associated with thrombocytosis, from 2016 to 2017. This was done to determine the incidence of thrombocytosis, and categorize the increase in platelet count with respect to severity. Of the 12,676 blood samples analyzed, 4.5% presented thrombocytosis (n = 573). Similar mean platelet counts were observed in all diagnosis or different categories of clinical conditions (neoplasms; gastrointestinal, endocrine, and ophthalmological diseases; trauma and surgery; dermatological, cardiac, neurological, infectious, respiratory, genitourinary, idiopathic, and multiple diseases; and pregnancy) with no significant difference (P ≥ 0.05). The disorders most commonly associated with thrombocytosis were gastrointestinal diseases, followed by neoplasms. Furthermore, increased platelet counts were observed in dogs treated with glucocorticoids and vincristine drugs. As for the degree of severity, extreme thrombocytosis occurred more frequently in the presence of gastrointestinal diseases. Resumo A trombocitose é o aumento do número de plaquetas por microlitro (µL) de sangue e contagens maiores que 1.000.000/µL podem estar associadas a sinais clínicos de sangramento ou trombose. Estudos anteriores sobre as causas subjacentes da trombocitose tem despertado o interesse quanto a sua importância clínica em cães. O presente estudo teve como objetivo analisar hemogramas de cães, com a finalidade de definir as principais doenças ou condições clínicas associadas à trombocitose dentro do período de dois anos, determinar a incidência e ainda categorizar as contagens de acordo com a severidade do aumento de plaquetas. De 12.676 hemogramas analisados, observou-se incidência de trombocitose de 4,5% (n=573). As contagens médias de plaquetas em todas as categorias de diagnósticos ou condições clínica (neoplasias, doenças gastrointestinais, endócrinas, oftalmológicas, traumas e cirurgias, dermatológicas, cardíacas, neurológicas, infecciosas, respiratórias, geniturinárias, idiopáticas, múltiplas e gestação) foram semelhantes, não havendo diferença significativa entre elas (P≥0,05). Os distúrbios mais comumente associados à trombocitose foram as doenças gastrointestinais seguidas de neoplasias. Além disso, os aumentos na contagem de plaquetas foram observados em cães tratados com glicocorticoides e vincristina. Quanto ao grau de severidade, a trombocitose extrema ocorreu mais frequentemente nas doenças gastrointestinais.

Background : Snakebites occur frequently among humans and animals. In Brazil, about 23,000 cases of snakebite involving humans were recorded in 2016, 11% of them caused by venomous snakes. In the region of Cuiaba, capital of Mato Grosso, the highest occurrence is of snakes of the genus Bothrops (81%), followed by Crotalus (4%). Bothrops venom has proteolytic, coagulant and hemorrhagic effects, whereas Crotalus venom is neurotoxic, myotoxic and nephrotoxic. This paper reports on three cases of Bothrops snakebites in dogs treated at the Veterinary Hospital of the Federal University of Mato Grosso in 2017. Cases : Three dogs were treated showing clinical signs of snakebite, possibly by Bothrops , since their owners reported that these are the snakes most frequently found where they live. This information is consistent with the known geographic distribution of snakes of the genus Bothrops in the state of Mato Grosso, Brazil. In the first report (Case 1 1 ), the patient was bitten on two different occasions in a five-month interval, and died presumably due to anaphylactic shock caused by the venom injected by a second snakebite (Case 1 2 ). In both episodes, the animal presented a swollen face and neck, dyspnea and eye bleeding, and these signs were more severe in the second episode. Case 2 was a dog with severe neck and chest swelling and pain. The owner of this dog reported that he had taken his dog for a walk in the countryside, along with the dog of Case 1, and had returned home with both dogs about three hours before the consultation. Case 3 was a Rottweiler living at a small family farm located 67 km from Cuiaba. The animal presented with slight swelling and pain in the left forelimb, and puncture wounds on the lower limb. Neutrophilic leukocytosis was observed in cases 1 and 3. In addition, Case 1 2 (the second episode of Case 1) presented with thrombocytopenia and increased prothrombin time and activated partial thromboplastin time. The dog of Case 2 presented no hematological or biochemical alteration. All the dogs received antivenom serum and supportive care. Discussion : Based on the clinical signs of local swelling with bleeding and absence of neurological signs, the snakebites were attributed to the genus Bothrops . In Case 1 2 , blood clotting tests showed non-coagulation. The greater severity of Case 1, which culminated in death, was attributed to anaphylaxis due to prior exposure or the larger amount of venom than that injected in Case 2, since the two injuries were inflicted in the same environment on the same day, probably by the same snake. Neutrophilic leukocytosis may be indicative of an inflammatory reaction, while thrombocytopenia and clotting changes are associated with the hemorrhagic activity of Bothrops venom. Death by snakebite is directly linked to the amount of venom injected, the envenomation site, and the time between the bite and the beginning of treatment. This explains the moderate signs presented by Cases 2 and 3, whose injuries were inflicted on the neck and forelimb, unlike the dog of Case 1, which was bitten on the head on two occasions. It was concluded that Bothrops venom causes systemic and local alterations characterized by pain, swelling, necrosis and bleeding. Early access to antivenom treatment is essential to neutralize clinical signs and prevent worsening of systemic poisoning. The determining factor in snakebite deaths is the amount of injected venom and the time elapsed between the injury and the beginning of treatment.

Platynosomum fastosum, which is distributed in tropical and subtropical regions, is the main parasite in the biliary system of domestic cats. Cats can be asymptomatic or show severe signs of cholangitis. Although the infection is reported throughout Brazil, only post-mortem studies are available from the Midwest region of the country. This cross-sectional study investigated the frequency of P. fastosum parasitism in domestic cats treated at the University Veterinary Hospital in Cuiaba city, Mato Grosso, Brazil, by three coproparasitological techniques (formalin-ether sedimentation, Faust, and Hoffmann), associating clinical, laboratory (haematological, biochemical) and ultrasonographic alterations. Based on the sample calculation, 171 cats were evaluated in the clinical and laboratory and a semi-structured epidemiological questionnaire was designed and used by the tutors. The prevalence observed was 26.90%, with no significant association with sex, reproductive status, age, hunting habit, access to the street, recent anthelmintic treatment, and the guardian's lack of knowledge regarding parasitism. Of the 171 cats evaluated, 55 (32.16%) showed clinical signs suggestive of cholangitis, with a significant association of this variable. Regarding the clinical and pathological variables, mild neutrophilia and elevated serum activity of alanine amino transferase and alkaline phosphatase was observed (but not significantly), while the mean values of eosinophils, leucocytes, total protein, albumin, and globulin were normal. The ultrasound findings were like those previously described. Thus, it was observed that P. fastosum occurs in moderate prevalence in cats from Cuiaba-MT, with a significant association with clinical signs and a regular agreement between the parasitological techniques employed.

A babesiose é uma zoonose causada por diferentes espécies de protozoários do gênero Babesia, que obrigatoriamente vivem no interior das células sanguíneas. Transimitida por carrapatos ixodídeos, ela pode acometer tanto animais domésticos, quanto silvestres. No Centro-Oeste, apesar de já existirem descrições da hemoparasitose, pouco se sabe referente à sua ocorrência, sendo a maioria dos estudos referentes à detecção por meio de diagnósticos moleculares. Apesar disso, o esfregaço sanguíneo mostra-se um método diagnóstico fundamental, principalmente, por ser um exame de triagem, viável, altamente conclusivo e de baixo custo. Este estudo objetivou enfatizar a importância do esfregaço sanguíneo como diagnóstico da enfermidade. Realizou-se um levantamento dos achados de Babesia spp. em esfregaços sanguíneos da rotina do HOVET – UFMT, durante o período de doze meses, bem como foi buscada a existência de um perfil hematológico, visando validar a suspeita mesmo em casos de baixa parasitemia. Foram processados 5.333 hemogramas caninos, destes, os que apresentaram o trofozoíto da Babesia spp. na citologia dos esfregaços foram submetidos à avaliação do resultado de seus exames. Foi constatado que mesmo em casos que a citologia resulte negativa, com o histórico clínico associado ao perfil hematológico é possível validar a suspeita da hemoparasitose. Verificou-se a ocorrência de 0,3% de babesiose em cães, sendo a maioria dos achados esporádicos. Os principais achados hematológicos foram anemia (68,75%) normocítica normocrômica (45,45%), trombocitopenia (87,5%), leucopenia (75%), eosinopenia (68,75%), linfopenia (87,5%) e monocitose (18,75%).

This study aimed to evaluate the effects of firocoxib for controlling experimentally-induced breakdown of the blood-aqueous barrier in healthy and Toxoplasma gondii -seropositive cats. Thirty two cats with no ocular abnormalities were used. Groups (n=8/each) were formed with healthy cats that received 5mg g-1 of oral firocoxib (FH) or no treatment (CH) on day 0; seropositive cats for anti -T. gondii specific immunoglobulin G (IgG) were grouped (n=8/each) and treated in a similar fashion (FT and CT). On day 1, cats of all groups received the same treatment protocol, and 1h later, aqueocentesis was performed under general anesthesia (M0). Following 1h, the same procedure was repeated (M1). Quantitation of aqueous humor total protein and prostaglandin E2 (PGE2) were determined. Aqueous samples of seropositive cats were tested for anti- T. gondii specific IgG. In M0, aqueous samples of CT showed a significantly higher concentration of PGE2 in comparison with other groups (P

Os exames de avaliação da coagulação sanguínea são utilizados para verificar a hemostasia em todas as suas fases e investigar a presença de doenças hemostáticas em pequenos animais. Diante da escassez e do alto valor aquisitivo de reagentes específicos para a avaliação da hemostasia em felinos, os reagentes humanos são utilizados em grande parte dos laboratórios clínicos veterinários, o que compromete a sensibilidade dos resultados. O presente trabalho teve como objetivo determinar valores de referência para o tempo de protrombina (TP) e tempo de tromboplastina parcial ativada (TTPa) para felinos domésticos (Felis catus), utilizando reagentes da marca Wiener Lab, padronizados para humanos. Amostras de sangue de 50 felinos adultos clinicamente sadios foram utilizadas. Os resultados obtidos foram de 3,9 ± 1,52 segundos para o TP, com valores mínimo e máximo de 3,52 e 4,34; e de 27,94 ± 1,77 segundos para TTPa, com valores mínimo e máximo de 27,50 e 28,48, respectivamente. Concluiu-se, que os valores obtidos neste experimento podem ser utilizados como referência para felinos, em testes manuais com kit humano da marca Wiener Lab.

This study aimed to assess Dog Erythrocyte Antigen (DEA) 1.1 in donor dogs at the Federal University of Mato Grosso, Cuiabá, Brazil, and review the relevant literature. The blood (60 samples; 1.5 mL volume, each) was collected in separate vacutainer tubes containing ethylenediaminetetraacetic acid and submitted for complete blood count; in addition, the samples were typed by RapidVet® based on agglutination due to specific interaction between DEA 1 antigen at the membrane surface of the erythrocyte and lyophilised murine monoclonal antibody on the test card. DEA1.1 positivity was observed in 81.6% (49 of 60) of test samples, while negative results were obtained in the remaining 18.3% (11 of 60). DEA 1.1 positive samples were comprised of 42.8% of purebred dogs and 38.3% of mixed breed dogs. With regard to sex in the DEA 1.1 positive group, 48.3% were male dogs and 33.3% were female dogs. The blood donor canine population showed high prevalence of DEA 1.1, which confirms that blood typing should be performed prior to blood transfusion in previously sensitised dogs.

This study aimed to investigate the effects of the systemic administration of acepromazine, tramadol and the association of both on intraocular pressure (IOP) and pupil diameter (PD) in young healthy cats. Cats were randomly allocated into three groups (n=10/each) and intramuscular acepromazine (AG), tramadol (TG) or acepromazine combined with tramadol (ATG) were injected. PD (electronic caliper) and IOP (applanation tonometry) were assessed before (baseline) and following 15, 30, 60, and 120 minutes of treatments. It was verified that in AG, PD decreased significantly from time point 30 to 120 (P=0.002), but such reduction did not differ significantly from baseline (P=0.89). In TG, PD increased significantly from the first 15 minutes, until the last time point of evaluation (P0.05). It can be concluded that tramadol alone or in association with acepromazine produced significant mydriasis for up to 120 minutes, without changing IOP values in normal cats. Results of this study suggested that tramadol alone or in association with acepromazine caused significant mydriasis and did not change IOP values in normal cats. Therefore, it may be considered a satisfactory pre-anesthetic combination for ophthalmic surgery in cats. However, further studies are warranted on the use of such protocols in cats with ophthalmic diseases undergoing ocular or intraocular surgery. RESUMO: Objetivou-se estudar os efeitos da administração sistêmica da acepromazina, do tramadol e da associação de ambos sobre a pressão intraocular (PIO) e o diâmetro pupilar (DP) em gatos saudáveis jovens. Os gatos foram aleatoriamente distribuídos em três grupos (n=10/cada) e tratados pela via intramuscular com acepromazina (GA), tramadol (GT) ou acepromazina combinada ao tramadol (GAT). O DP (paquimetria eletrônica) e a PIO (tonometria de aplanação) foram mensurados antes (basal) e após 15, 30, 60, e 120 minutos após a administração dos tratamentos. Constatou-se que no GA, o DP reduziu significativamente a partir do 30˚ até o 120˚ minuto de avaliação (P=0.02), mas sem diferir significativamente em relação ao basal (P=0,89). No GT, o DP se elevou significativamente desde 15˚ minuto, até o último período de avaliação (P˂0.001). No GAT, o DP se elevou de forma significativa do 30˚ ao 120˚ minuto em comparação ao basal (P0,05). Conclui-se que o tramadol, administrado de forma isolada ou em associação à acepromazina, produz midríase de até 120 minutos, sem alterar os valores da PIO em gatos saudáveis. Dessa forma, esse protocolo pré-anestésico pode ser considerado uma alternativa para cirurgia oftálmica em gatos. Todavia, os resultados desse protocolo em gatos com doença oftálmica, que necessitem de cirurgia, devem ser avaliados em estudos futuros.

EnglishObesity has been increasing in cats andis associated with metabolic and cardiovascular diseases. The association of these alterations can trigger the onset of metabolic syndrome (MS). Therefore, this study aimed to analyze the serum levels of glucose, fructosamine, cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, very low-density lipoprotein (VLDL), andalanine aminotransferase of cats and to identify the possibility of MS,as well as to evaluate changes in arterial pressure. Thirty-seven cats were classified by ECC and morphometric measurements, namely, 15 obese, 12 overweight, and 10 controls. Nocat manifested MS. Only VLDL had a statistically significant difference (P Key words: obesity,cats; hypertension; dyslipidemia portuguesNos ultimos anos, tem sido observado um aumento na incidencia de obesidade em gatos, sendo associado a doencas metabolicas e cardiovasculares e a associacao dessas alteracoes pode desencadear o surgimento de sindrome metabolica. Diante disto, o objetivo deste trabalho foi analisar os niveis sericos de glicose, frutosamina, colesterol, triglicerideos, HDL, LDL, VLDL e ALT dos gatos e identificar uma possivel presenca de sindrome metabolica nos mesmos, alem de avaliar a ocorrencia de alteracoes na pressao arterial. Utilizaram-se 37 gatos, classificados atraves de ECC e medidas morfometricas em 15 obesos, 12 sobrepesos e 10 controles. Nenhum gato do estudo manifestou sindrome metabolica. Apenas o VLDL obteve diferenca estatisticamente significativa (P Palavras-chave: obesidade; gatos; hipertensao; dislipidemia

RESUMO: Na doença renal crônica (DRC), a hipertensão arterial é uma das complicações mais comuns, que pode estar associada a liberação dos peptídeos natriuréticos e a hipertrofia ventricular no homem. Em cães os peptídeos natriuréticos são estudados como biomarcadores cardíacos, mas ainda se desconhece sua função em cães com DRC. Desta forma, este estudo teve como objetivo relacionar a concentração sérica de peptídeo natriurético B aos achados de pressão arterial, eletrocardiográficos, eletrolíticos, hematológicos e bioquímicos séricos e urinários em cães nos distintos estágios da DRC. Foram constatadas diferenças significativas na relação de proteína creatinina urinária (UPC, p=0,006), anemia, creatinina e uréia sérica (p

Chronic anaemia is one of the most severe complications of chronic kidney disease, contributing to morbidity and mortality caused by the disease; therefore, bone marrow cytological evaluation is needed to monitor the progression of anaemia. This study aimed to correlate the anaemia in dogs at different stages of chronic kidney disease with their serum biochemistry, myelogram results and serum erythropoietin findings. Sixty-three dogs were grouped according to International Renal Interest Society (IRIS) classification in stages 1, 2, 3 and 4. Haematologic, serum and urinary biochemistry and serum erythropoietin were performed for comparison with the findings of bone marrow cytology obtained by aspiration of the manubrium. Cytological findings for erythroid hypoplasia were described in 93.65% of dogs, and the anaemia was observed in 84.1% of them. The haematological findings were correlated with azotaemia (p

The transfusion of blood components is common in a veterinary clinic; however, the safety of this therapeutic measure cannot always be guaranteed. Studies show a high risk of haemoparasite transmission during blood transfusion in canines. These parasites include Leishmania chagasi, Anaplasma platys, and Ehrlichia canis, which are endemic to the city of Cuiabá. This study aimed to evaluate the occurrence of L. chagasi, Trypanosoma cruzi, Babesia (canis) vogeli, A. platys, and E. canis in canine blood donor candidates, and identify possible factors associated with the infection of these agents. Sixty-six canines were evaluated using serologic and molecular tests, for the presence of the Leishmania species. While one canine sample showed a positive result for L. chagasi with indirect fluorescent antibody test, with titer of 1:40, and seven canine samples were positive using DPP, all other samples were negative when using PCR and ELISA. All canines were negative for T. cruzi when using PCR. The B. (c.) vogeli infection was identified in one canine and A. platys was identified in six canines. E. canis was identified in 17 canines, with a prevalence of 25.7%. There were no significant factors associated with the infection of the pathogens investigated. Given the observation of infection, even in the absence of clinical symptoms, emphasis must be placed on the need for the use of more sensitive and specific diagnostic methods for the screening of donor canines. Administração de hemocomponentes é uma prática de rotina na clínica veterinária, entretanto, a segurança nessa medida terapêutica nem sempre é garantida. Estudos mostram risco elevado na transmissão de hemoparasitos pela transfusão sanguínea em cães. Dentre estes, Leishmania chagasi, Anaplasma platys e Ehrlichia canis são endêmicas no município de Cuiabá. Este estudo teve como objetivo avaliar a ocorrência de L. chagasi, Trypanosoma cruzi, Babesia (canis) vogeli, A. platys e E. canis em cães candidatos a doadores de sangue e identificar os possíveis fatores associados à infecção pelos referidos agentes. Dos 66 cães avaliados por exames sorológicos e moleculares para Leishmania sp, um cão reagiu na IFI na titulação 1:40 para L. chagasi., sete no DPP, e todos negativos na PCR e ELISA. Todos os cães foram negativos na PCR para T. cruzi. Foi identificada infecção por Babesia (c.) vogeli em apenas um cão e seis para A. platys. E. canis foi diagnosticado em 17 cães, com ocorrência de 25,7%. Não foram observados fatores associados significativamente à infecção pelos patógenos pesquisados. Diante da observação da infecção mesmo na ausência de alterações clínicas, ressalta-se o uso de métodos diagnósticos mais sensíveis e específicos na triagem de cães doadores.

Resumo Este trabalho teve como objetivo avaliar a influência do tempo e da temperatura de armazenamento sobre os valores das dosagens bioquímicas de ALT em 60 amostras de soro e plasma de cães, divididas em dois grupos: GR (grupo refrigerado, a 5 ºC) e GC (grupo congelado, a -20 ºC), por até 96 horas analisadas em seis tempos e até 60 dias, analisadas em cinco tempos, respectivamente. O estudo mostrou que o congelamento e a refrigeração não provocaram variações entre as amostras (p< 0,005). Os dados obtidos nas dosagens bioquímicas revelaram que o soro e o plasma com EDTA mantiveram-se próximos dos valores basais, ou seja, não se alteraram significantemente ao longo do tempo de conservação e nas diferentes temperaturas estudadas. Abstract This study aimed to evaluate the effect of time and storage temperature on the values of ALT biochemical measurements in 60 samples of serum and plasma of dogs divided into two groups: RG (refrigerated group, at 5 ºC) and FG (frozen group, at -20 ºC) for 96 hours analyzed at six moments and up to 60 days examined at five moments, respectively. The study showed that the effect of freezing and cooling caused no variation between the samples (p

Infections of the genitourinary system are among the most frequent in the clinical routine of small animals. Treatment with the most appropriate antimicrobial therapy, according to the uropathogen susceptibility test, can avoid the spread of bacterial resistance to antimicrobials. A clinical study was performed in 32 canines, of both sexes and differing ages, who attended the Veterinary Teaching Hospital. Urine samples underwent culture, with the objective of evaluating urinary tract infection in dogs with renal disease, identifying the associated bacterial pathogens, and verifying their antimicrobial susceptibility in vitro. Urine culture was positive in 10 dogs, mostly males, with no predisposition for breed, and a mean age of 8.28 years. Most of the urinary tract infections (UTIs) were monobacterial, with the most common microorganisms being Pseudomonas sp. and Staphylococcus sp. The antimicrobials imipenem and meropenem had the best overall sensitivity profile, and ampicillin showed the highest resistance. The variation in epidemiological profiles, and susceptibility to uropathogens, reinforces the importance of the veterinarian in the prevention and control of infection, in addition to the need for further research to identify new antimicrobial agents.

The Lower Urinary Tract Disease Feline (FLUTD) are frequent in clinical routine of this species is due to various disease processes characterized by hematuria, dysuria, urinary frequency, strangury, periuria and urethral obstruction, affecting mainly sedentary cats, living in the home and with low intake of water. In order to check the main clinical, laboratory and ultrasound in cases of FLUTD, 26 cats were available at the Veterinary Hospital of UFMT being 16 with FLUTD and ten healthy, noting that all the sick animals had azotemia, average of serum total calcium decreased and elevation in serum phosphorus. Bacterial growth observed in one third of cases, three possibly due to bladder catheterization, as were recurrent cases. The sonographic findings were compatible with frames of obstruction, such as severe distension of the urinary bladder and debris in suspension, dilated ureters and urethra. As Doenças do Trato Urinário Inferior Felino (DTUIF) são frequentes na rotina clínica desta espécie, é decorrente de diversos processos mórbidos caracterizados por hematúria, disúria, polaciúria, estrangúria, periúria e obstrução uretral, que afetam principalmente felinos sedentários, de vivência intradomiciliar e com baixa ingestão de água. Com o objetivo de verificar as principais alterações clínicas, laboratoriais e ultrassonográficas nos casos de DTUIF, foram examinados 26 felinos no Hospital Veterinário da UFMT, sendo, 16 com DTUIF e dez hígidos, notando-se que todos os animais doentes apresentaram azotemia, valor médio da concentração sérica de cálcio total diminuído e elevação na concentração sérica de fósforo. Crescimento bacteriano foi observado em um terço dos casos, sendo três possivelmente decorrentes de cateterização vesical, já que eram casos recidivantes. Os achados ultrassonográficos foram compatíveis com quadros de obstrução, tais como: distensão acentuada da vesícula urinária e debris em suspensão, dilatação de ureteres e uretra.