1. Activation of the toll-like receptor 2 signaling pathway by GAPDH from bacterial strain RD055328.
- Author
-
Kurata A, Takeuchi S, Fujiwara R, Tamura K, Imai T, Yamasaki-Yashiki S, Onuma H, Fukuta Y, Shirasaka N, and Uegaki K
- Subjects
- RAW 264.7 Cells, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Recombinant Proteins pharmacology, Immunoglobulin A immunology, Interleukin-6 immunology, Adjuvants, Immunologic genetics, Adjuvants, Immunologic isolation & purification, Adjuvants, Immunologic pharmacology, Animals, Mice, NF-kappa B immunology, Transcriptional Activation drug effects, Signal Transduction drug effects, Toll-Like Receptor 2 immunology, Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) genetics, Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) isolation & purification, Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) pharmacology, Lactobacillaceae classification, Lactobacillaceae enzymology, Lactobacillaceae genetics, Lactobacillaceae isolation & purification, Bacterial Proteins genetics, Bacterial Proteins pharmacology
- Abstract
We characterized the membrane vesicle fraction (RD-MV fraction) from bacterial strain RD055328, which is related to members of the genus Companilactobacillus and Lactiplantibacillus plantarum. RD-MVs and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were detected in the RD-MV fraction. Immunoglobulin A (IgA) was produced by Peyer's patch cells following the addition of the RD-MV fraction. In the presence of the RD-MV fraction, RAW264 cells produced the pro-inflammatory cytokine IL-6. Recombinant GAPDH probably induced the production of IL-6 by RAW264 cells via superficial toll-like receptor 2 (TLR2) recognition. A confocal laser scanning microscopy image analysis indicated that RD-MVs and GAPDH were taken up by RAW264 cells. GAPDH wrapped around RAW264 cells. We suggest that GAPDH from strain RD055328 enhanced the production of IgA by acquired immune cells via the production of IL-6 by innate immune cells through TLR2 signal transduction., (© The Author(s) 2023. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
- Published
- 2023
- Full Text
- View/download PDF