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Objective: To investigate the induction and differentiation potential of ADSCs by tissue culture method,and to preliminary study on the origin of ADSCs. Methods: Using adipose tissue culture method to culture human ADSCs. The third generation of ADSCs for the adipogenic and osteogenesis differentiation, and staining by oil red O and alizarin red S. HE staining was performed after the seventh day culture of adipose tissue. Results: The primary human ADSCs were successfully cultured with adipose tissue culture method. ADSCs cultured to the eighth generation, still maintained a good proliferation ability and cell morphology. ADSCs can be successfully induced into adipose cells and bone cells. ADSCs were mainly distributed around the mesenchymal vascular and connective tissue, by HE staining of adipose tissue after seven days of culture. Conclusion: The cells that were cultured with adipose tissue have the potential to adipogenic and osteogenesis differentiation. The ADSCs were mainly distributed around the mesenchymal vascular and connective tissue. [ABSTRACT FROM AUTHOR]

Sirtuins comprise a family of deacetylase enzymes that catalyze the removal of an acetyl moiety from the ɛ-amino group of lysine residues within protein targets. Sirtuin 1(SIRT1), a NAD+ dependent class III histone deacetylase is involved in a variety of human disorders such as obesity, type II diabetes, cancer and aging. Inhibition of SIRT1 could be useful for cancer treatment while activators can be useful for longevity and treating metabolic disorders. Hence we undertook an effort to design both inhibitors and activators using structure-based drug design techniques and report here the biological proof of concept. In this paper, we report diverse small molecule inhibitors with a potential to attenuate cancer growth designed based on high-throughput virtual screening and docking using the crystal structure of SIRT1. And small molecule activators with potential to suppress adipogenesis differentiation indicating their usefulness in obesity control was designed based on a homology model of SIRT1 activator domain. [ABSTRACT FROM AUTHOR]

Background and Objectives: Mesenchymal stem cells (MSCs) have the multipotent capacity to differentiate into multiple tissue lineages as well as to self-renew, which is the main origin of adipocytes. IL6/IL6R pathway exerts a significant role in tissue regeneration and cell differentiation. Whereas, the underlying mechanism between IL6/IL6R pathway and MSCs adipogenesis differentiation remains elusive., Methods: MSCs from healthy donors were cultured in adipogenesis differentiation medium for 0∼14 days, during which their adipogenesis differentiation degree was evaluated by Oil Red O staining. The expression of IL6R was detected in MSCs during adipogenesis differentiation. Knockdown and overexpression of IL6R were respectively performed using siRNA and lentivirus to investigate its effect on MSCs adipogenesis differentiation. The adipogenesis marker genes expression and MAPK pathway activation were detected by Western blotting. The role of P38 pathway in the adipogenesis differentiation of MSCs was determined using the specific inhibitor SB203580., Results: The expression of IL6 and IL6R increased during adipogenesis differentiation in MSCs, which were positively correlated with Oil Red O quantification result. Knockdown and overexpression experiments demonstrated a positive correlation between the expressions of IL6R and MSCs adipogenesis differentiation, accompanied by same trend of P38 phosphorylation. Besides, the specific P38 inhibitor SB203580 markedly inhibited the adipogenesis differentiation potential of MSCs., Conclusions: This study reveals IL6R facilitates the adiogenesis differentiation of MSCs via activating P38 pathway.