1. Adipose tissue sensitivity to radiation exposure
- Author
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Bénédicte Prunet-Marcassus, Luc Pénicaud, Sylvain Galvani, Louis Casteilla, Sandy Bour, Sandrine Poglio, Béatrice Cousin, Mireille André, Développement et Communication Chimique chez les Insectes ( DCCI ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Delon, Viviane
- Subjects
Pathology ,medicine.medical_specialty ,Cellular differentiation ,Population ,Experimental/metabolism/*pathology ,Adipose tissue ,Connective tissue ,Cell Proliferation/*radiation effects ,Biology ,Polymerase Chain Reaction ,Pathology and Forensic Medicine ,Mice ,Oxidative Stress/radiation effects ,medicine ,Adipocytes ,In Situ Nick-End Labeling ,Autologous transplantation ,Animals ,Stem Cells/metabolism/pathology/radiation effects ,Progenitor cell ,education ,Radiation Injuries ,Cell Proliferation ,education.field_of_study ,Stem Cells ,Adipocytes/cytology/metabolism/*radiation effects ,Cell Differentiation ,Total body irradiation ,Flow Cytometry ,Immunohistochemistry ,Adipose Tissue/cytology/*radiation effects ,Oxidative Stress ,Radiation Injuries, Experimental ,Cell Differentiation/*radiation effects ,medicine.anatomical_structure ,Phenotype ,Adipose Tissue ,Stem cell ,Regular Articles - Abstract
1525-2191 (Electronic) Journal Article Research Support, Non-U.S. Gov't; Treatment of cancer using radiation can be significantly compromised by the development of severe acute and late damage to normal tissue. Treatments that either reduce the risk and severity of damage or that facilitate the healing of radiation injuries are being developed, including autologous adipose tissue grafts to repair tissue defects or involutional disorders that result from tumor resection. Adipose tissue is specialized in energy storage and contains different cell types, including preadipocytes, which could be used for autologous transplantation. It has long been considered a poorly proliferative connective tissue; however, the acute effects of ionizing radiation on adipose tissue have not been investigated. Therefore, the aim of this study was to characterize the alterations induced in adipose tissue by total body irradiation. A severe decrease in proliferating cells, as well as a significant increase in apoptotic cells, was observed in vivo in inguinal fat pads following irradiation. Additionally, irradiation altered the hematopoietic population. Decreases in the proliferation and differentiation capacities of non-hematopoietic progenitors were also observed following irradiation. Together, these data demonstrate that subcutaneous adipose tissue is very sensitive to irradiation, leading to a profound alteration of its developmental potential. This damage could also alter the reconstructive properties of adipose tissue and, therefore, calls into question its use in autologous fat transfer following radiotherapy.
- Published
- 2009