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A multistate experiment involving universities in IL, IN, KY, and MN was conducted as a part of the research efforts by the North-Central Coordinating Committee-42 on swine nutrition. The null hypothesis that there are no differences in the standardized ileal digestibility (SID) of amino acids (AA) among different sources of bakery meal was tested. Eleven sources of bakery meal were procured from swine-producing states in the United States and each source was included in one diet as the sole source of AA. A N-free diet was prepared as well. Diets were prepared in one batch and divided into four sub-batches that were subsequently distributed to the four participating universities. At each university, diets were fed to 12 pigs that had a T- cannula installed in the distal ileum. Pigs were allotted to incomplete Latin square designs with 12 pigs and 4, 5, or 6 periods for a total of 21 replicate pigs per diet. Each period lasted 7 d with ileal digesta being collected from the cannulas on days 6 and 7. Samples were analyzed for AA and the SID of each AA was calculated. Results indicated that there were differences (P < 0.001) in the SID of all AA except Pro among the 11 sources of bakery meal. The differences in SID of AA observed in this experiment were greater than what is usually observed among sources of the same ingredient, indicating that there is more variability among sources of bakery meal than among different sources of other ingredients. This is likely a consequence of different raw materials being used in the production of different sources of bakery meal. Regardless of source of bakery meal, the AA with the least SID was Lys indicating that some of the raw materials in the product streams used to generate the bakery meals may have been overheated. Additionally, the Lys:crude protein ratio in each source of bakery meal was not a good predictor of the SID of Lys, which likely reflects the different raw materials being included in the different meals. In conclusion, the SID of AA varies among different sources of bakery meal and the SID of Lys is less than the SID of all other indispensable AA.

The objectives were to develop equations for predicting ileal digestible phosphorus (P) based on nonphytate P, phytate P, calcium to nonphytate P ratio, and exogenous phytase concentrations in broiler diets and to evaluate the accuracy of the models. The modified Michaelis–Menten model was used to predict apparent ileal digestible (AID) or standardized ileal digestible P in broiler diets. Two different methods were used to analyze 25 published ileal digestible P data to evaluate the accuracy of the equation for predicting AID P. The first method assessed mean and linear biases. While the mean bias was significant (P < 0.05), the linear bias was not significant. The second method involved regressing the 25 AID P data against those predicted by the current model. The results showed that the intercept and slope of the regression were not statistically different from 0 and 1, respectively. This suggests that the current equation accurately represents the test data. In conclusion, the accuracy of the model depends on validation processes. Considering that the current model was developed using various phytase products, it would be preferable to use an equation that is based on a single phytase product if requisite quantity of data is available. [ABSTRACT FROM AUTHOR]

Coccidiosis is an infectious parasitic disease. Increasing drug resistance and variable vaccine effectiveness have stimulated interests in developing alternate control strategies. Our research studied the effects of dietary supplementation of multienzyme (phytase, xylanase, β-glucanase, amylase, hemicellulase, and pectinase) in broiler chickens infected with coccidial pathogens by assessing (i) nutrient utilization, immunological response, and gut health-related indicators in small intestine; and (ii) intestinal microbiome modulations. One-day-old male Ross 708 chicks were assigned to 4 treatments in a 2 × 2 factorial arrangement comprising of 0 or 50 g·kg−1 multienzyme and oral challenge with phosphate buffer saline or mixed Eimeria spp. oocysts (250000 E. acervulina, 50000 E. maxima, and 50000 E. tenella). Multienzyme reduced (P < 0.05) Eimeria-induced loss in feed efficiency and nutrient utilization, partially explained by reduced decrease of b0,+ amino acid transporter in jejunum. Multienzyme suppressed (P < 0.05) the overexpression of interleukin-8 in duodenum and jejunum and ameliorated (P = 0.05) the decreased expression of antioxidant heme oxygenase 1 in ileum induced by Eimeria infection. Multienzyme facilitated (P < 0.01) the bloom of short-chain fatty acid-producing and fiber-degrading microbes. Multienzyme supplementation partly mitigated the adverse effects of Eimeria infection through multiple mechanisms: improving nutrient utilization, reducing local inflammation, and restoring microbial homeostasis. [ABSTRACT FROM AUTHOR]

The digestible energy (DE) and metabolizable energy (ME) in faba beans (FB) and field peas (FP) fed to growing pigs were estimated by the difference procedure in two experiments using the total collection method. Twenty-four barrows with mean initial body weight (BW) of 20.0 kg (SD = 1.13) and 20.4 kg (SD = 0.56) in the first (Exp. 1) and second experiments (Exp. 2), respectively, were assigned to three dietary treatments in a randomized complete block design with BW as a blocking factor. A reference diet was prepared using corn, soybean meal, and soybean oil as the energy-contributing ingredients. Organic FB and DS Admiral FP (FPD) in Exp. 1 and Hampton FP (FPH) and 4010 FP (FP4) in Exp. 2 were included at 30% in the reference diet. In Exp. 1, the determined DE and ME were 3772 and 3606 kcal·kg−1 dry matter (DM) in FB and 3683 and 3589 kcal·kg−1 DM in FPD, respectively. In Exp. 2, the respective DE and ME were 4164 and 4014 kcal·kg−1 DM in FPH and 3574 and 3467 kcal·kg−1 DM in FP4. In conclusion, the determined ME values for FB and FP were 77% to 90% of gross energy. [ABSTRACT FROM AUTHOR]

In the current study, we hypothesized that an increase in dietary ileal indigestible protein concentration induces an increase in hindgut nitrogen utilization and nitrogen excretion and a shift in fecal microbiota in growing pigs, when compared to pigs given a high total protein diet. Three diets were prepared: 1) standard protein diet based on corn and soybean meal, 2) high-indigestible protein diet in which autoclaved, low-digestible soybean meal replaced soybean meal in the first diet, and 3) high protein diet where the inclusion rate of soybean meal was greater than that of the other diets. The 3 diets were fed to 18 barrows that were fitted with T-cannula at the ileo-cecal junction (initial body weight = 63.4 ± 8.0 kg) in a randomized complete block design with body weight as a blocking factor. Pigs were individually housed in pens and the experiment lasted for 23 d. On days 7 and 21, fecal samples were collected by rectal massage for microbiota analysis. Grab samples of feces were collected on days 20 and 21, and ileal digesta were collected on days 22 and 23 for the determination of energy and nitrogen utilization. Lower apparent ileal digestibility of nitrogen in the high-indigestible protein diet containing autoclaved soybean meal resulted in greater ileal indigestible nitrogen concentration (P  < 0.05). Apparent total tract digestibility of nitrogen was lower (P  < 0.05), and correspondingly nitrogen concentration and daily fecal nitrogen output were greater (P  < 0.05) in the high-indigestible protein diet compared with the other diets. Apparent post-ileal digestibility and hindgut disappearance of nitrogen and gross energy were the greatest (P  < 0.05) in the high protein diet, whereas a statistical difference was not observed in those variables between the standard protein diet and the high-indigestible protein diet. Beta diversity metrics of feces in the high-indigestible protein diet on day 21 were different (q  < 0.05) from those in the other two diets, which indicates a shift in microbial communities. According to the results of the DESeq2, the direction of microbiota shift induced by the high-indigestible protein diet may have reduced fiber utilization in the hindgut. In conclusion, an increase in dietary ileal indigestible protein concentration increased fecal nitrogen excretion and shifted fecal microbial communities but did not increase nitrogen utilization in the hindgut. [ABSTRACT FROM AUTHOR]

Objective: An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in fish meal (FM) and blood-derived protein sources including spray-dried porcine plasma (SDPP), porcine red blood cells (PRBC), and blood meal (BM) fed to growing pigs. Methods: Ten barrows (mean initial body weight of 22.1±1.54 kg) surgically fitted with T-cannulas at the distal ileum were allotted to a duplicated 5×4 incomplete Latin square design with 5 experimental diets and 4 periods. Four experimental diets were prepared to contain FM, SDPP, PRBC, or BM as the sole source of nitrogen. A nitrogen-free diet was prepared and included to estimate the basal ileal endogenous losses of AA. For the 7-day experimental period, pigs were fed for 5 days as adaptation, and ileal digesta samples were collected for 9 hours on days 6 and 7. Results: The SID of crude protein in BM (48.0%) was less (p<0.05) than in FM, SDPP, and PRBC (83.4%, 83.9%, and 87.3%, respectively). Pigs fed the diet containing BM had less (p<0.05) SID of AA, except isoleucine and proline, than those fed the diet containing FM, SDPP, or PRBC. Among FM, SDPP, and PRBC, there was no difference in the SID of crude protein and all AA, except isoleucine. The SID of isoleucine in PRBC and BM (62.7% and 48.3%, respectively) was less (p<0.05) than in FM and SDPP (88.0% and 84.9%, respectively). The SID of lysine in FM, SDPP, PRBC, and BM was 85.4%, 84.9%, 89.7%, and 51.9%, respectively. Conclusion: The SID of most AA was not different among FM, SDPP, and PRBC, but BM had lower SID of most AA than FM, SDPP, and PRBC. [ABSTRACT FROM AUTHOR]