16 results on '"Adema Ribic"'
Search Results
2. Preterm birth accelerates the maturation of spontaneous and resting activity in the visual cortex
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Isabelle F. Witteveen, Emily McCoy, Troy D. Holsworth, Catherine Z. Shen, Winnie Chang, Madelyn G. Nance, Allison R. Belkowitz, Avery Dougald, Meghan H. Puglia, and Adema Ribic
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preterm (birth) ,neurodevelopment ,visual activity ,inhibition ,EEG ,neuron ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Prematurity is among the leading risks for poor neurocognitive outcomes. The brains of preterm infants show alterations in structure and electrical activity, but the underlying circuit mechanisms are unclear. To address this, we performed a cross-species study of the electrophysiological activity in the visual cortices of prematurely born infants and mice. Using electroencephalography (EEG) in a sample of healthy preterm (N = 29) and term (N = 28) infants, we found that the maturation of the aperiodic EEG component was accelerated in the preterm cohort, with a significantly flatter 1/f slope when compared to the term infants. The flatter slope was a result of decreased spectral power in the theta and alpha bands and was correlated with the degree of prematurity. To determine the circuit and cellular changes that potentially mediate the changes in 1/f slope after preterm birth, we used in vivo electrophysiology in preterm mice and found that, similar to infants, preterm birth results in a flattened 1/f slope. We analyzed neuronal activity in the visual cortex of preterm (N = 6) and term (N = 9) mice and found suppressed spontaneous firing of neurons. Using immunohistochemistry, we further found an accelerated maturation of inhibitory circuits. In both preterm mice and infants, the functional maturation of the cortex was accelerated, underscoring birth as a critical checkpoint in cortical maturation. Our study points to a potential mechanism of preterm birth-related changes in resting neural activity, highlighting the utility of a cross-species approach in studying the neural circuit mechanisms of preterm birth-related neurodevelopmental conditions.
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- 2023
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3. Stability in the Face of Change: Lifelong Experience-Dependent Plasticity in the Sensory Cortex
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Adema Ribic
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cortex ,plasticity ,interneuron ,experience ,synapse ,cholinergic ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Plasticity is a fundamental property of the nervous system that enables its adaptations to the ever-changing environment. Heightened plasticity typical for developing circuits facilitates their robust experience-dependent functional maturation. This plasticity wanes during adolescence to permit the stabilization of mature brain function, but abundant evidence supports that adult circuits exhibit both transient and long-term experience-induced plasticity. Cortical plasticity has been extensively studied throughout the life span in sensory systems and the main distinction between development and adulthood arising from these studies is the concept that passive exposure to relevant information is sufficient to drive robust plasticity early in life, while higher-order attentional mechanisms are necessary to drive plastic changes in adults. Recent work in the primary visual and auditory cortices began to define the circuit mechanisms that govern these processes and enable continuous adaptation to the environment, with transient circuit disinhibition emerging as a common prerequisite for both developmental and adult plasticity. Drawing from studies in visual and auditory systems, this review article summarizes recent reports on the circuit and cellular mechanisms of experience-driven plasticity in the developing and adult brains and emphasizes the similarities and differences between them. The benefits of distinct plasticity mechanisms used at different ages are discussed in the context of sensory learning, as well as their relationship to maladaptive plasticity and neurodevelopmental brain disorders. Knowledge gaps and avenues for future work are highlighted, and these will hopefully motivate future research in these areas, particularly those about the learning of complex skills during development.
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- 2020
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4. Emerging Roles of Synapse Organizers in the Regulation of Critical Periods
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Adema Ribic and Thomas Biederer
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Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Experience remodels cortical connectivity during developmental windows called critical periods. Experience-dependent regulation of synaptic strength during these periods establishes circuit functions that are stabilized as critical period plasticity wanes. These processes have been extensively studied in the developing visual cortex, where critical period opening and closure are orchestrated by the assembly, maturation, and strengthening of distinct synapse types. The synaptic specificity of these processes points towards the involvement of distinct molecular pathways. Attractive candidates are pre- and postsynaptic transmembrane proteins that form adhesive complexes across the synaptic cleft. These synapse-organizing proteins control synapse development and maintenance and modulate structural and functional properties of synapses. Recent evidence suggests that they have pivotal roles in the onset and closure of the critical period for vision. In this review, we describe roles of synapse-organizing adhesion molecules in the regulation of visual critical period plasticity and we discuss the potential they offer to restore circuit functions in amblyopia and other neurodevelopmental disorders.
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- 2019
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5. Synapse-Selective Control of Cortical Maturation and Plasticity by Parvalbumin-Autonomous Action of SynCAM 1
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Adema Ribic, Michael C. Crair, and Thomas Biederer
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Biology (General) ,QH301-705.5 - Abstract
Summary: Cortical plasticity peaks early in life and tapers in adulthood, as exemplified in the primary visual cortex (V1), wherein brief loss of vision in one eye reduces cortical responses to inputs from that eye during the critical period but not in adulthood. The synaptic locus of cortical plasticity and the cell-autonomous synaptic factors determining critical periods remain unclear. We here demonstrate that the immunoglobulin protein Synaptic Cell Adhesion Molecule 1 (SynCAM 1/Cadm1) is regulated by visual experience and limits V1 plasticity. Loss of SynCAM 1 selectively reduces the number of thalamocortical inputs onto parvalbumin (PV+) interneurons, impairing the maturation of feedforward inhibition in V1. SynCAM 1 acts in PV+ interneurons to actively restrict cortical plasticity, and brief PV+-specific knockdown of SynCAM 1 in adult visual cortex restores juvenile-like plasticity. These results identify a synapse-specific, cell-autonomous mechanism for thalamocortical visual circuit maturation and closure of the visual critical period. : Ribic et al. show that cortical plasticity is actively restricted by the synapse-organizing molecule SynCAM 1. The protein acts in parvalbumin interneurons to recruit excitatory thalamocortical terminals. This controls the maturation of inhibition and actively limits cortical plasticity, revealing a synaptic locus for closure of cortical critical periods. Keywords: synapse, SynCAM, Cadm, visual cortex, critical period, plasticity, parvalbumin, thalamocortical inputs
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- 2019
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6. Excitatory Synaptic Drive and Feedforward Inhibition in the Hippocampal CA3 Circuit Are Regulated by SynCAM 1
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Kellie A. Park, Yuegao Huang, Adema Ribic, Fahmeed Hyder, Thomas Biederer, Fabian M Laage Gaupp, Daniel Coman, and Chris G. Dulla
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Male ,0301 basic medicine ,Mossy fiber (hippocampus) ,Time Factors ,Interneuron ,Conditioning, Classical ,Immunoglobulins ,Neural Inhibition ,In Vitro Techniques ,Hippocampal formation ,GABA Antagonists ,Synapse ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Postsynaptic potential ,Neural Pathways ,medicine ,Animals ,Mice, Knockout ,Memory Disorders ,biology ,Chemistry ,musculoskeletal, neural, and ocular physiology ,General Neuroscience ,Cell Adhesion Molecule-1 ,Articles ,Fear ,Synaptic Potentials ,CA3 Region, Hippocampal ,Mice, Inbred C57BL ,Pyridazines ,Parvalbumins ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,nervous system ,Synapses ,biology.protein ,Excitatory postsynaptic potential ,Female ,Cell Adhesion Molecules ,Neuroscience ,030217 neurology & neurosurgery ,Parvalbumin - Abstract
Select adhesion proteins control the development of synapses and modulate their structural and functional properties. Despite these important roles, the extent to which different synapse-organizing mechanisms act across brain regions to establish connectivity and regulate network properties is incompletely understood. Further, their functional roles in different neuronal populations remain to be defined. Here, we applied diffusion tensor imaging (DTI), a modality of magnetic resonance imaging (MRI), to map connectivity changes in knock-out (KO) mice lacking the synaptogenic cell adhesion protein SynCAM 1. This identified reduced fractional anisotropy in the hippocampal CA3 area in absence of SynCAM 1. In agreement, mossy fiber refinement in CA3 was impaired in SynCAM 1 KO mice. Mossy fibers make excitatory inputs onto postsynaptic specializations of CA3 pyramidal neurons termed thorny excrescences and these structures were smaller in the absence of SynCAM 1. However, the most prevalent targets of mossy fibers are GABAergic interneurons and SynCAM 1 loss unexpectedly reduced the number of excitatory terminals onto parvalbumin (PV)-positive interneurons in CA3. SynCAM 1 KO mice additionally exhibited lower postsynaptic GluA1 expression in these PV-positive interneurons. These synaptic imbalances in SynCAM 1 KO mice resulted in CA3 disinhibition, in agreement with reduced feedforward inhibition in this network in the absence of SynCAM 1-dependent excitatory drive onto interneurons. In turn, mice lacking SynCAM 1 were impaired in memory tasks involving CA3. Our results support that SynCAM 1 modulates excitatory mossy fiber inputs onto both interneurons and principal neurons in the hippocampal CA3 area to balance network excitability. SIGNIFICANCE STATEMENT This study advances our understanding of synapse-organizing mechanisms on two levels. First, the data support that synaptogenic proteins guide connectivity and can function in distinct brain regions even if they are expressed broadly. Second, the results demonstrate that a synaptogenic process that controls excitatory inputs to both pyramidal neurons and interneurons can balance excitation and inhibition. Specifically, the study reveals that hippocampal CA3 connectivity is modulated by the synapse-organizing adhesion protein SynCAM 1 and identifies a novel, SynCAM 1-dependent mechanism that controls excitatory inputs onto parvalbumin-positive interneurons. This enables SynCAM 1 to regulate feedforward inhibition and set network excitability. Further, we show that diffusion tensor imaging is sensitive to these cellular refinements affecting neuronal connectivity.
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- 2016
7. Synaptic Connectivity and Cortical Maturation Are Promoted by the ω-3 Fatty Acid Docosahexaenoic Acid
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Katherine Watters, Adema Ribic, Malik Abouleish, Thomas Biederer, Benjamin M. Bader, Olaf H.-U. Schroeder, Beatrice E. Carbone, and Seth Vogel
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genetic structures ,Docosahexaenoic Acids ,Cognitive Neuroscience ,Visual Acuity ,Biology ,Synapse ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Postsynaptic potential ,In vivo ,Cortex (anatomy) ,Neural Pathways ,medicine ,Animals ,Cells, Cultured ,030304 developmental biology ,Visual Cortex ,chemistry.chemical_classification ,Neurons ,0303 health sciences ,food and beverages ,Fatty acid ,Dendrites ,Cell biology ,Mice, Inbred C57BL ,Electrophysiology ,Visual cortex ,medicine.anatomical_structure ,chemistry ,Docosahexaenoic acid ,Synapses ,lipids (amino acids, peptides, and proteins) ,030217 neurology & neurosurgery - Abstract
Brain development is likely impacted by micronutrients. This is supported by the effects of the ω-3 fatty acid docosahexaenoic acid (DHA) during early neuronal differentiation, when it increases neurite growth. Aiming to delineate DHA roles in postnatal stages, we selected the visual cortex due to its stereotypic maturation. Immunohistochemistry showed that young mice that received dietary DHA from birth exhibited more abundant presynaptic and postsynaptic specializations. DHA also increased density and size of synapses in a dose-dependent manner in cultured neurons. In addition, dendritic arbors of neurons treated with DHA were more complex. In agreement with improved connectivity, DHA enhanced physiological parameters of network maturation in vitro, including bursting strength and oscillatory behavior. Aiming to analyze functional maturation of the cortex, we performed in vivo electrophysiological recordings from awake mice to measure responses to patterned visual inputs. Dietary DHA robustly promoted the developmental increase in visual acuity, without altering light sensitivity. The visual acuity of DHA-supplemented animals continued to improve even after their cortex had matured and DHA abolished the acuity plateau. Our findings show that the ω-3 fatty acid DHA promotes synaptic connectivity and cortical processing. These results provide evidence that micronutrients can support the maturation of neuronal networks.
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- 2018
8. Synapse-selective control of cortical maturation and plasticity engages an interneuron-autonomous synaptic switch
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Michael C. Crair, Thomas Biederer, and Adema Ribic
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Gene knockdown ,genetic structures ,Interneuron ,Selective control ,Plasticity ,Biology ,Visual cortex ,medicine.anatomical_structure ,Neuroplasticity ,medicine ,Excitatory postsynaptic potential ,biology.protein ,Neuroscience ,Parvalbumin - Abstract
HighlightsThe synaptogenic molecule SynCAM 1 is selectively regulated by visual experienceSynCAM 1 controls thalamic input onto cortical Parvalbumin (PV+) interneuronsPV+-specific knockdown of SynCAM 1 arrests maturation of cortical inhibitionThalamic excitation onto PV+ interneurons is essential for critical period closureeTOC BlurbRibic et al. show that network plasticity in both young and adult cortex is restricted by the synapse organizing molecule SynCAM 1. On a cellular level, it functions in Parvalbumin-positive interneurons to recruit thalamocortical terminals. This controls the maturation of inhibitory drive and restricts plasticity in the cortex. These results reveal the synaptic locus of cortical plasticity and identify the first cell-autonomous synaptic factor for closure of cortical critical periods.SummaryBrain plasticity peaks early in life and tapers in adulthood. This is exemplified in the primary visual cortex, where brief loss of vision to one eye abrogates cortical responses to inputs from that eye during the critical period, but not in adulthood. The synaptic locus of critical period plasticity and the cell-autonomous synaptic factors timing these periods remain unclear. We here demonstrate that the immunoglobulin protein Synaptic Cell Adhesion Molecule 1 (SynCAM 1/Cadm1) is regulated by visual experience and limits visual cortex plasticity. SynCAM 1 selectively controls the number of excitatory thalamocortical (TC) inputs onto Parvalbumin (PV+) interneurons and loss of SynCAM 1 in turn impairs the maturation of TC-driven feed-forward inhibition. SynCAM 1 acts in cortical PV+ interneurons to perform these functions and its PV+-specific knockdown prevents the age-related plasticity decline. These results identify a synapse type-specific, cell-autonomous mechanism that governs circuit maturation and closes the visual critical period.
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- 2018
9. Structural organization and function of mouse photoreceptor ribbon synapses involve the immunoglobulin protein synaptic cell adhesion molecule 1
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Xinran Liu, Thomas Biederer, Michael C. Crair, and Adema Ribic
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Retina ,genetic structures ,Cell adhesion molecule ,General Neuroscience ,Immunoelectron microscopy ,Ribbon synapse ,Biology ,Synapse ,medicine.anatomical_structure ,Excitatory postsynaptic potential ,medicine ,Immunoglobulin superfamily ,sense organs ,Retinal Rod Photoreceptor Cells ,Neuroscience - Abstract
Adhesive interactions in the retina instruct the developmental specification of inner retinal layers. However, potential roles of adhesion in the development and function of photoreceptor synapses remain incompletely understood. This contrasts with our understanding of synapse development in the CNS, which can be guided by select adhesion molecules such as the Synaptic Cell Adhesion Molecule 1 (SynCAM 1/CADM1/nectin-like 2 protein). This immunoglobulin superfamily protein modulates the development and plasticity of classical excitatory synapses. We show here by immunoelectron microscopy and immunoblotting that SynCAM 1 is expressed on mouse rod photoreceptors and their terminals in the outer nuclear and plexiform layers in a developmentally regulated manner. Expression of SynCAM 1 on rods is low in early postnatal stages (P3-P7) but increases after eye opening (P14). In support of functional roles in the photoreceptors, electroretinogram recordings demonstrate impaired responses to light stimulation in SynCAM 1 knockout (KO) mice. In addition, the structural integrity of synapses in the OPL requires SynCAM 1. Quantitative ultrastructural analysis of SynCAM 1 KO retina measured fewer fully assembled, triadic rod ribbon synapses. Furthermore, rod synapse ribbons are shortened in KO mice, and protein levels of Ribeye, a major structural component of ribbons, are reduced in SynCAM 1 KO retina. Together, our results implicate SynCAM 1 in the synaptic organization of the rod visual pathway and provide evidence for novel roles of synaptic adhesion in the structural and functional integrity of ribbon synapses.
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- 2014
10. Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture
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Adema Ribic and Mathias De Roo
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0301 basic medicine ,Nervous system ,Dendritic spine ,Synaptogenesis ,Hippocampus ,Context (language use) ,Biology ,Synapse ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Structural plasticity ,medicine ,Neuroscience ,030217 neurology & neurosurgery ,Organotypic slice - Abstract
Understanding the rules of synapse dynamics in the context of development, learning, and nervous system disorders is an important part of several fields of neuroscience. Despite significant methodological advances, observations of structural dynamics of synapses still present a significant experimental challenge. In this chapter we describe a set of techniques that allow repetitive observations of synaptic structures in vitro in organotypic cultures of rodent hippocampus. We describe culturing of slices, transfection with reporter-carrying plasmids, repetitive imaging of dendritic spines with confocal laser scanning microscopy and analysis of spine morphology dynamics.
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- 2016
11. Stress Upregulates TPH1 but not TPH2 mRNA in the Rat Dorsal Raphe Nucleus: Identification of Two TPH2 mRNA Splice Variants
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Nashat Abumaria, Adema Ribic, Gabriele Flügge, Christoph Anacker, and Eberhard Fuchs
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Male ,Restraint, Physical ,medicine.medical_specialty ,Tryptophan Hydroxylase ,Biology ,Serotonergic ,Pineal Gland ,Cellular and Molecular Neuroscience ,Pineal gland ,Dorsal raphe nucleus ,Stress, Physiological ,Internal medicine ,Adrenal Glands ,medicine ,Animals ,Protein Isoforms ,RNA, Messenger ,Rats, Wistar ,TPH1 ,TPH2 ,Organ Size ,Cell Biology ,General Medicine ,Tryptophan hydroxylase ,Molecular biology ,Rats ,Alternative Splicing ,Endocrinology ,medicine.anatomical_structure ,Gene Expression Regulation ,Raphe Nuclei ,Serotonin ,Nucleic Acid Amplification Techniques ,Nucleus - Abstract
Serotonin is implicated in stress-related psychopathologies. Two isoforms of the rate-limiting enzyme of serotonin biosynthesis, tryptophan hydroxylase, TPH1 and TPH2, are known. We show here that in the rat dorsal raphe nucleus (DRN), the nucleus that contains the highest number of 5-HT neurons in the brain, TPH1 mRNA reveals a low level of expression but is detectable both by quantitative real-time PCR and in situ hybridization whereas in the pineal gland (PiG), TPH1 mRNA is strongly expressed. To examine effects of stress on TPH expression we exposed male Wistar rats to daily restraint stress for 1 week. As shown by quantitative real-time PCR, TPH1 mRNA is 2.5-fold upregulated by the stress in DRN but not in PiG. Using 3'-RACE, we identified two TPH2 mRNA splice variants in the rat DRN which differ in the length of their 3'-untranslated regions (UTRs). TPH2b (with a short 3'-UTR) is the predominant variant in the DRN, whereas TPH2a (with a longer 3'-UTR) shows a low abundance in this nucleus. In the PiG, only TPH2b is detectable revealing a low level of expression. Expression of both TPH2 splice variants is not affected by stress, neither in DRN nor in the PiG. These data indicate that TPH1 in the serotonergic neurons of the DRN might be relevant for stress-induced psychopathologies.
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- 2008
12. A short N-terminal domain of HDAC4 preserves photoreceptors and restores visual function in retinitis pigmentosa
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Hong-Ping Xu, Adema Ribic, Xianjun Zhu, Bo Chen, Xinzheng Guo, Michael C. Crair, Yu Zhou, Thomas Biederer, Shaobin Wang, and Ethan J. Mohns
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Genotype ,genetic structures ,Transgene ,General Physics and Astronomy ,Mice, Transgenic ,Biology ,medicine.disease_cause ,Histone Deacetylases ,Article ,General Biochemistry, Genetics and Molecular Biology ,Mice ,Retinitis pigmentosa ,Electroretinography ,medicine ,Animals ,Humans ,Photoreceptor Cells ,Vision, Ocular ,Regulation of gene expression ,Mutation ,Multidisciplinary ,Gene therapy of the human retina ,medicine.diagnostic_test ,HEK 293 cells ,General Chemistry ,medicine.disease ,HDAC4 ,Molecular biology ,eye diseases ,Protein Structure, Tertiary ,3. Good health ,Cell biology ,Repressor Proteins ,HEK293 Cells ,Gene Expression Regulation ,sense organs ,Gene Deletion ,Retinitis Pigmentosa - Abstract
Retinitis pigmentosa is a leading cause of inherited blindness, with no effective treatment currently available. Mutations primarily in genes expressed in rod photoreceptors lead to early rod death, followed by a slower phase of cone photoreceptor death. Rd1 mice provide an invaluable animal model to evaluate therapies for the disease. We previously reported that overexpression of histone deacetylase 4 (HDAC4) prolongs rod survival in rd1 mice. Here we report a key role of a short N-terminal domain of HDAC4 in photoreceptor protection. Expression of this domain suppresses multiple cell death pathways in photoreceptor degeneration, and preserves even more rd1 rods than the full-length HDAC4 protein. Expression of a short N-terminal domain of HDAC4 as a transgene in mice carrying the rd1 mutation also prolongs the survival of cone photoreceptors, and partially restores visual function. Our results may facilitate the design of a small protein therapy for some forms of retinitis pigmentosa.
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- 2015
13. Immune Privilege Revisited: The Roles of Neuronal MHC Class I Molecules in Brain Development and Plasticity
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Adema Ribic
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- 2012
14. Differential effects of maternal immune activation and juvenile stress on anxiety-like behaviour and physiology in adult rats: no evidence for the 'double-hit hypothesis'
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Nicole Yee, Eberhard Fuchs, Adema Ribic, and Christina Coenen de Roo
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Male ,Elevated plus maze ,medicine.medical_specialty ,Reflex, Startle ,Offspring ,Physiology ,Anxiety ,Rats, Sprague-Dawley ,03 medical and health sciences ,Behavioral Neuroscience ,chemistry.chemical_compound ,0302 clinical medicine ,Corticosterone ,Pregnancy ,Internal medicine ,Adrenal Glands ,medicine ,Animals ,Maze Learning ,Prepulse inhibition ,030304 developmental biology ,0303 health sciences ,Analysis of Variance ,Body Weight ,medicine.disease ,Rats ,Disease Models, Animal ,Inhibition, Psychological ,Endocrinology ,Poly I-C ,chemistry ,Acoustic Stimulation ,Animals, Newborn ,Prenatal Exposure Delayed Effects ,Gestation ,Cytokines ,Female ,medicine.symptom ,Psychology ,030217 neurology & neurosurgery ,Stress, Psychological ,Psychopathology - Abstract
Environmental disruptions can influence neurodevelopment during pre- and postnatal periods. Given such a large time window of opportunity for insult, the "double-hit hypothesis" proposes that exposure to an environmental challenge may impact development such that an individual becomes vulnerable to developing a psychopathology, which then manifests upon exposure to a second challenge later in life. The present study in male rats utilized the framework of the "double-hit hypothesis" to investigate potential compounding effects of maternal immune activation (MIA) during pregnancy and exposure of offspring to stress during juvenility on physiological and behavioural indications of anxiety in adulthood. We used an established rat model of MIA via maternal treatment with polyinosinic:polycytidylic acid (poly I:C) on gestation day 15 in combination with a model of juvenile stress (applied ages 27-29 d) in offspring to explore potential interacting/additive effects. First, we confirmed our employment of the MIA model by replicating previous findings that prenatal treatment with poly I:C caused deficits in sensorimotor gating in adult offspring, as measured by prepulse inhibition. Juvenile stress, on the other hand, had no effect on prepulse inhibition. In terms of anxiety-related behaviour and physiology, we found that prenatal poly I:C alone or in combination with juvenile stress had no effects on body weight, adrenal weight, and plasma concentration of corticosterone and cytokines in adult rats. MIA and juvenile stress increased anxiety-related behaviour on the elevated plus maze, but did so independently of each other. In all, our findings do not support an interaction between MIA and juvenile stress in terms of producing marked changes related to anxiety-like behaviour in adulthood.
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- 2011
15. Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey
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Gabriele Flügge, Eberhard Fuchs, Adema Ribic, Lutz Walter, Kerstin Mätz-Rensing, and Christina Schlumbohm
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Male ,genetic structures ,Cognitive Neuroscience ,Synaptogenesis ,Genes, MHC Class I ,lcsh:RC346-429 ,Eye Enucleation ,03 medical and health sciences ,Behavioral Neuroscience ,0302 clinical medicine ,biology.animal ,MHC class I ,medicine ,Animals ,Premovement neuronal activity ,lcsh:Neurology. Diseases of the nervous system ,Biological Psychiatry ,Visual Cortex ,030304 developmental biology ,Neurons ,0303 health sciences ,biology ,Research ,Age Factors ,Gene Expression Regulation, Developmental ,Marmoset ,Callithrix ,General Medicine ,biology.organism_classification ,Monocular deprivation ,Visual cortex ,medicine.anatomical_structure ,biology.protein ,Neuroscience ,030217 neurology & neurosurgery ,Ocular dominance column - Abstract
Background Several recent studies have highlighted the important role of immunity-related molecules in synaptic plasticity processes in the developing and adult mammalian brains. It has been suggested that neuronal MHCI (major histocompatibility complex class I) genes play a role in the refinement and pruning of synapses in the developing visual system. As a fast evolutionary rate may generate distinct properties of molecules in different mammalian species, we studied the expression of MHCI molecules in a nonhuman primate, the common marmoset monkey (Callithrix jacchus). Methods and results Analysis of expression levels of MHCI molecules in the developing visual cortex of the common marmoset monkeys revealed a distinct spatio-temporal pattern. High levels of expression were detected very early in postnatal development, at a stage when synaptogenesis takes place and ocular dominance columns are formed. To determine whether the expression of MHCI molecules is regulated by retinal activity, animals were subjected to monocular enucleation. Levels of MHCI heavy chain subunit transcripts in the visual cortex were found to be elevated in response to monocular enucleation. Furthermore, MHCI heavy chain immunoreactivity revealed a banded pattern in layer IV of the visual cortex in enucleated animals, which was not observed in control animals. This pattern of immunoreactivity indicated that higher expression levels were associated with retinal activity coming from the intact eye. Conclusions These data demonstrate that, in the nonhuman primate brain, expression of MHCI molecules is regulated by neuronal activity. Moreover, this study extends previous findings by suggesting a role for neuronal MHCI molecules during synaptogenesis in the visual cortex.
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- 2011
16. Neuronal MHC Class I Molecules are Involved in Excitatory Synaptic Transmission at the Hippocampal Mossy Fiber Synapses of Marmoset Monkeys
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Adema Ribic, Eberhard Fuchs, Mingyue Zhang, Gabriele Flügge, Barbara Uchanska-Ziegler, Christina Schlumbohm, Weiqi Zhang, Lutz Walter, and Kerstin Mätz-Rensing
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Male ,599.8 ,CA3 ,Hippocampus ,Hippocampal formation ,Synaptic Transmission ,0302 clinical medicine ,VGAT ,Biomedicine ,Animal Anatomy / Morphology / Histology ,Neurosciences ,Original Research ,Hippocampal mossy fiber ,Neurons ,0303 health sciences ,biology ,GFAP ,sEPSC ,Marmoset ,Callithrix ,General Medicine ,CA3 Region, Hippocampal ,Protein Transport ,VGlut1 ,Neuronal plasticity ,Mossy Fibers, Hippocampal ,Excitatory postsynaptic potential ,Female ,Mossy fiber (hippocampus) ,Presynaptic Terminals ,In Vitro Techniques ,Antibodies ,Cell Line ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,biology.animal ,Animals ,Humans ,VGlut 2 ,030304 developmental biology ,Calbindin ,Piccolo ,PSD95 ,Histocompatibility Antigens Class I ,Cell Biology ,biology.organism_classification ,nervous system ,Synapses ,Synaptic plasticity ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Several recent studies suggested a role for neuronal major histocompatibility complex class I (MHCI) molecules in certain forms of synaptic plasticity in the hippocampus of rodents. Here, we report for the first time on the expression pattern and functional properties of MHCI molecules in the hippocampus of a nonhuman primate, the common marmoset monkey (Callithrix jacchus). We detected a presynaptic, mossy fiber-specific localization of MHCI proteins within the marmoset hippocampus. MHCI molecules were present in the large, VGlut1-positive, mossy fiber terminals, which provide input to CA3 pyramidal neurons. Furthermore, whole-cell recordings of CA3 pyramidal neurons in acute hippocampal slices of the common marmoset demonstrated that application of antibodies which specifically block MHCI proteins caused a significant decrease in the frequency, and a transient increase in the amplitude, of spontaneous excitatory postsynaptic currents (sEPSCs) in CA3 pyramidal neurons. These findings add to previous studies on neuronal MHCI molecules by describing their expression and localization in the primate hippocampus and by implicating them in plasticity-related processes at the mossy fiber–CA3 synapses. In addition, our results suggest significant interspecies differences in the localization of neuronal MHCI molecules in the hippocampus of mice and marmosets, as well as in their potential function in these species. Electronic supplementary material The online version of this article (doi:10.1007/s10571-010-9510-3) contains supplementary material, which is available to authorized users.
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