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Key message: The response of herbivore abundance to birch genotypic diversity varies depending on the host specificity of the herbivores. Specialist, but not generalist, herbivores were affected by birch genotypic diversity. Biological control is an important ecosystem service mediated through plant diversity. Responses of herbivore abundance to plant genotypic diversity may depend on host specificity, especially the host-feeding type. We conducted a common-garden experiment by manipulating plot-level genotypic diversity (number of source populations per plot) of Erman's birch (Betula ermanii) to understand how birch genotypic diversity affected the abundance of three herbivore feeding types (chewers, galls, and suckers). Specifically, we investigated whether the effects of plant genotypic diversity on herbivore abundance were additive or non-additive. Furthermore, we examined this mechanism as a possible change in plant phenotype (plant productivity and/or leaf traits) and/or herbivore foraging behavior (associational resistance with neighboring plant individuals). We found that genotypic diversity had a significant positive effect on condensed tannin concentration; however, it did not affect plant growth. There was no effect of increasing birch genotypic diversity on the density of chewers (generalists) and chewing herbivory, whereas the density of galls and suckers (specialists) decreased significantly. A negative non-additive effect was observed with regard to gall density and a negative additive effect on sucker density was observed. Gall density was not related to the increase in condensed tannins affected by birch genotypic diversity. This implies that associational resistance is more likely to explain the negative non-additive effects on gall density. Our study shows that the loss of birch genotypic diversity due to shrinking population sizes will strongly affect specialist herbivores in the boreal forests of Hokkaido. [ABSTRACT FROM AUTHOR]

Abstract Modelling of solar irradiation is paramount to renewable energy management. This warrants the inclusion of additive effects to predict solar irradiation. Modelling of additive effects to solar irradiation can improve the forecasting accuracy of prediction frameworks. To help develop the frameworks, this current study modelled the additive effects using non-parametric quantile regression (QR). The approach applies quantile splines to approximate non-parametric components when finding the best relationships between covariates and the response variable. However, some additive effects are perceived as linear. Thus, the study included the partial linearly additive quantile regression model (PLAQR) in the quest to find how best the additive effects can be modelled. As a result, a comparative investigation on the forecasting performances of the PLAQR, an additive quantile regression (AQR) model and the new quantile generalised additive model (QGAM) using out-of-sample and probabilistic forecasting metric evaluations was done. Forecasted density plots, Murphy diagrams and results from the Diebold–Mariano (DM) hypothesis test were also analysed. The density plot, the curves on the Murphy diagram and most metric scores computed for the QGAM were slightly better than for the PLAQR and AQR models. That is, even though the DM test indicates that the PLAQR and AQR models are less accurate than the QGAM, we could not conclude an outright greater forecasting performance of the QGAM than the PLAQR or AQR models. However, in situations of probabilistic forecasting metric preferences, each model can be prioritised to be applied to the metric where it performed slightly the best. The three models performed differently in different locations, but the location was not a significant factor in their performances. In contrast, forecasting horizon and sample size influenced model performance differently in the three additive models. The performance variations also depended on the metric being evaluated. Therefore, the study has established the best forecasting horizons and sample sizes for the different metrics. It was finally concluded that a 20% forecasting horizon and a minimum sample size of 10000 data points are ideal when modelling additive effects of solar irradiation using non-parametric QR.

Nematodes have a negative impact on crop production and yield. The use of synthetic formulations to control plant parasitic nematodes carries both environmental and human health risks. As these agrochemicals are gradually being phased out, recent research has been focused on finding more environmentally friendly, plant-based alternatives. This study aims to investigate the effectiveness of botanicals, used alone or in artificial blends, in paralyzing Meloidogyne incognita second-stage juveniles (J2s) immersed in test solutions or exposed to vapors. We tested thymol, trans-anethole, and two lavender essential oils, referred to as LEOA and LEOB, which vary in their flower and stem compositions. We also employed in our study Melia azedarach aqueous extract (MWE), already proven to have considerable nematicidal activity. According to our findings, all treatments used individually exhibited considerable efficacy, even LEOA and LEOB first reported herein. In addition, all blends exhibited significant synergism, and the best-performing were trans-anethole/thymol, being synergic to paralyzing J2s for up to two days, and trans-anethole/LEOB as well as trans-anethole/MWE, provoking irreversible paralysis since the first day of J2 immersion in test solutions. Most importantly, the blend of trans-anethole with LEOA displayed the best effective synergism against M. incognita both for immersion and fumigation methods. Lastly, the chemical composition analysis displayed linalyl acetate and β-linalool as the major components of LEOA and β-linalool and eucalyptol as the major components of LEOB. [ABSTRACT FROM AUTHOR]

Modelling of solar irradiation is paramount to renewable energy management. This warrants the inclusion of additive effects to predict solar irradiation. Modelling of additive effects to solar irradiation can improve the forecasting accuracy of prediction frameworks. To help develop the frameworks, this current study modelled the additive effects using non-parametric quantile regression (QR). The approach applies quantile splines to approximate non-parametric components when finding the best relationships between covariates and the response variable. However, some additive effects are perceived as linear. Thus, the study included the partial linearly additive quantile regression model (PLAQR) in the quest to find how best the additive effects can be modelled. As a result, a comparative investigation on the forecasting performances of the PLAQR, an additive quantile regression (AQR) model and the new quantile generalised additive model (QGAM) using out-of-sample and probabilistic forecasting metric evaluations was done. Forecasted density plots, Murphy diagrams and results from the Diebold–Mariano (DM) hypothesis test were also analysed. The density plot, the curves on the Murphy diagram and most metric scores computed for the QGAM were slightly better than for the PLAQR and AQR models. That is, even though the DM test indicates that the PLAQR and AQR models are less accurate than the QGAM, we could not conclude an outright greater forecasting performance of the QGAM than the PLAQR or AQR models. However, in situations of probabilistic forecasting metric preferences, each model can be prioritised to be applied to the metric where it performed slightly the best. The three models performed differently in different locations, but the location was not a significant factor in their performances. In contrast, forecasting horizon and sample size influenced model performance differently in the three additive models. The performance variations also depended on the metric being evaluated. Therefore, the study has established the best forecasting horizons and sample sizes for the different metrics. It was finally concluded that a 20% forecasting horizon and a minimum sample size of 10000 data points are ideal when modelling additive effects of solar irradiation using non-parametric QR. [ABSTRACT FROM AUTHOR]

Prior research investigating situations involving one trustor and multiple trustees often examines how a trustor's trust in one party affects their amount of trust in another party. This paper fills a gap by predicting the effects of trust. The Perceived Influence (PI) Model of Trust is an individual-level model focused on the perceptions of a trustor. It builds upon the Mayer et al. (1995) model by integrating insights from literature on task interdependence and expanding to two trustees. The PI model describes and explains three possibilities for how a trustor's trust in two trustees may combine to form a sense of aggregate multi-trustee trust via: (1) additive effects, such that the trustor's trust in each of the trustees has independent effects on the aggregate; (2) compulsory effects, such that increasing the amount of trust in one trustee increases the effect of trust in the other trustee; and (3) compensatory effects, such that increasing trust in one trustee decreases the effect of trust in the other trustee. We propose that the theoretical mechanism explaining which of these three possibilities takes place is the trustor's perceived influence of the trustees, which is tightly linked to perceptions of task requirements necessary to attenuate the trustor's risk. The PI model begins to fill an important gap in the literature pertaining to pervasive, but rarely considered, multi-trustee situations, and proposes the importance of trustor perceptions of trustee influence and task requirements for future models of trust. [ABSTRACT FROM AUTHOR]

Abstract [Objective] The study aims to reveal the molecular mechanism of the formation of seed numbers per pod in Brassica juncea L. and Brassica crops, and lay a foundation for improving the yield and breeding of B. juncea. [Methods] In this study, 221 recombinant inbred lines (RILs) were engineered using the B.juncea L. We analyzed the additive quantitative trait loci (QTL), additive/additive epistatic effects, and genotype/environment interaction effects on the seed numbers per siliqua in mustard-type rapeseed across five distinct environmental settings. [Results] We identified a total of seven additive QTL associated with the number of seeds per siliqua, predominantly localized on chromosomes A02, A03, A05, A08, B02, and B03 of mustard-type rapeseed. The magnitude of additive effects ranged from -11.642 4 to 4.524 6, among them, qSS2-71 had the highest additive effect and transmission rate, reaching -11.642 4 and 14.44%, respectively. The remaining six additive QTL exhibited less pronounced additive effects and heritabilities. Moreover, the study detected seven pairs of additive/additive QTL interactions influencing the number of seeds per siliqua and their corresponding interactions with the environment. The effects of these epistatic QTL interactions ranged from -4.930 8 to 4.193 6, with the genetic effects of these interactions across different environments was approximately zero. Broad-sense heritability of the number of seeds per siliqua trait was 80.98%, while the narrow-sense heritability was 30.98%. [Conclusion] Although the number of seeds per siliqua in mustard-type rapeseed is influenced by environmental factors, the additive effects governing this trait appear to be minimally impacted by the environment, and the epistatic QTL interactions are not substantial

Over the past decades, the TIM‐barrel fold has served as a model system for the exploration of how changes in protein sequences affect their structural, stability, and functional characteristics, and moreover, how this information can be leveraged to design proteins from the ground up. After numerous attempts to design de novo proteins with this specific fold, sTIM11 was the first validated de novo design of an idealized four‐fold symmetric TIM barrel. Subsequent efforts to enhance the stability of this initial design resulted in the development of DeNovoTIMs, a family of de novo TIM barrels with various stabilizing mutations. In this study, we present an investigation into the biophysical and thermodynamic effects upon introducing a varying number of stabilizing mutations per quarter along the sequence of a four‐fold symmetric TIM barrel. We compared the base design DeNovoTIM0 without any stabilizing mutations with variants containing mutations in one, two, three, and all four quarters—designated TIM1q, TIM2q, TIM3q, and DeNovoTIM6, respectively. This analysis revealed a stepwise and nonlinear change in the thermodynamic properties that correlated with the number of mutated quarters, suggesting positive nonadditive effects. To shed light on the significance of the location of stabilized quarters, we engineered two variants of TIM2q which contain the same number of mutations but positioned in different quarter locations. Characterization of these TIM2q variants revealed that the mutations exhibit varying effects on the overall protein stability, contingent upon the specific region in which they are introduced. These findings emphasize that the amount and location of stabilized interfaces among the four quarters play a crucial role in shaping the conformational stability of these four‐fold symmetric TIM barrels. Analysis of de novo proteins, as described in this study, enhances our understanding of how sequence variations can finely modulate stability in both naturally occurring and computationally designed proteins. [ABSTRACT FROM AUTHOR]

Abstract Introduction Chronic low back pain often comprises mixed pain types and involves multiple factors. Therefore, we hypothesized that the systemic transdermal formulation of diclofenac sodium (DF systemic patch), which is effective for nociceptive pain, and an α2δ Ca2+ channel ligand, which is effective for neuropathic pain, would have additive effects in the treatment of chronic low back pain. Methods From among participants in a randomized, double-blind, placebo-controlled study of DF systemic patch (75 or 150 mg) applied once daily for 2 weeks in patients with chronic low back pain, we performed a subpopulation analysis of those who were concomitantly treated with an α2δ Ca2+ channel ligand during the study period. The efficacy endpoint was pain intensity score on a visual analog scale (VAS). Results The difference (95% confidence interval) in the least square mean pain VAS score between patients in the 150-mg combination group, who were treated with 150-mg DF systemic patch and an α2δ Ca2+ channel ligand (n = 11), and those in the non-combination group, who were treated with placebo patch and α2δ Ca2+ channel ligand (n = 22), was − 15.09 mm (− 26.45, − 3.73). Because the upper limit of the 95% confidence interval was less than zero, this result indicates that the pain VAS score improved more in the 150-mg combination group than in the non-combination group (placebo group). Conclusions The combination of the DF systemic patch and an α2δ Ca2+ channel ligand may be more effective than α2δ Ca2+ channel ligand monotherapy for controlling chronic low back pain. Trial registration numbers: JPRN-JapicCTI-205134 and jRCT2080225040.

Background. This work aimed at using Hayman’s methodology to study the inheritance of some quantitative traits, such as the number of pods per plant, pod length, mean pod weight, and yield per plant in 15 diallel hybrids of six green bean cultivars.Materials and methods. The research was carried out at the Experimental Center of Universidad Nacional de Colombia – Sede Palmira (CEUNP). A randomized complete block experimental design with four replications was used.Results. For most of the traits, except yield per plant, the additive-dominant model was adequate. Non-additive effects with overdominance controlled the number of pods per plant, pod length, and mean pod weight. Most of the characters presented an unequal proportion of positive and negative genes in the loci (H1 < H2). The asymmetric distribution of genes in the parents (H2/4H1) was below the maximum value (0.25), except for the character “number of pods per plant”. The KD/KR ratio confirmed for most of the traits that there was an excess of recessive alleles over dominant alleles. Dominance effects (h2) for most characters suggested that the substantial contribution of dominance was not due to heterogeneity of loci in these characters. The narrow-sense heritability was moderate to low. The correlation coefficient r (Yr; Wr + Vr) indicated that dominant genes were responsible for the increased number of pods per plant and recessive genes for increased pod length and mean pod weight.Conclusions. Conventional breeding methods like pedigree selection could be employed to improve the characters “pod length” and “mean pod weight”, and for the number of pods per plant, management of segregating populations should employ the single-seeded descent method.

Human epidermal growth factor receptor 2 (ErbB2/HER2) is a tyrosine kinase receptor that is overexpressed in 25% of primary human breast cancers, as well as in multiple other cancers. HER2‐targeted therapies improved progression‐free and overall survival in patients with HER2+ breast cancers. However, associated resistance mechanisms and toxicity highlight the need for new therapeutic approaches for these cancers. We recently established that, in normal cells, HER2 is stabilized in a catalytically repressed state by direct interaction with members of the ezrin/radixin/moesin (ERM) family. In HER2‐overexpressing tumors, the low expression of moesin contributes to the aberrant activation of HER2. Through a screen designed to find moesin‐mimicking compounds, we identified ebselen oxide. We show that ebselen oxide, and some derivatives, conferred an efficient allosteric inhibition of overexpressed HER2, as well as mutated and truncated oncogenic forms of HER2, which are resistant to current therapies. Ebselen oxide selectively inhibited anchorage‐dependent and ‐independent proliferation of HER2+ cancer cells and showed a significant benefit in combination with current anti‐HER2 therapeutic agents. Finally, ebselen oxide significantly blocked HER2+ breast tumor progression in vivo. Collectively, these data provide evidence that ebselen oxide is a newly identified allosteric inhibitor of HER2 to be considered for therapeutic intervention on HER2+ cancers.

Introduction: Chronic low back pain often comprises mixed pain types and involves multiple factors. Therefore, we hypothesized that the systemic transdermal formulation of diclofenac sodium (DF systemic patch), which is effective for nociceptive pain, and an α2δ Ca2+ channel ligand, which is effective for neuropathic pain, would have additive effects in the treatment of chronic low back pain. Methods: From among participants in a randomized, double-blind, placebo-controlled study of DF systemic patch (75 or 150 mg) applied once daily for 2 weeks in patients with chronic low back pain, we performed a subpopulation analysis of those who were concomitantly treated with an α2δ Ca2+ channel ligand during the study period. The efficacy endpoint was pain intensity score on a visual analog scale (VAS). Results: The difference (95% confidence interval) in the least square mean pain VAS score between patients in the 150-mg combination group, who were treated with 150-mg DF systemic patch and an α2δ Ca2+ channel ligand (n = 11), and those in the non-combination group, who were treated with placebo patch and α2δ Ca2+ channel ligand (n = 22), was − 15.09 mm (− 26.45, − 3.73). Because the upper limit of the 95% confidence interval was less than zero, this result indicates that the pain VAS score improved more in the 150-mg combination group than in the non-combination group (placebo group). Conclusions: The combination of the DF systemic patch and an α2δ Ca2+ channel ligand may be more effective than α2δ Ca2+ channel ligand monotherapy for controlling chronic low back pain. Trial registration numbers: JPRN-JapicCTI-205134 and jRCT2080225040. [ABSTRACT FROM AUTHOR]

The phenomenon of heterosis has provided the most important genetic tools for crop yield improvement. Identification of specific parental combinations capable of producing the highest level of heterotic effects in F1 has immense value for commercial exploitation of heterosis. The objective of this study was to assess the combining ability variances and superiority in twenty one combinations developed by crossing seven maize (Zea mays L.) inbred lines in half diallel fashion design. The resulting 21 hybrids, along with two check hybrids, were evaluated in Randomised Complete Block Design, in three locations during 2022 season in Egypt. The results showed that both additive and non-additive gene actions were important in controlling all the measured traits. The General Combining Ability (GCA) variance was found to be greater than Specific Combining Ability (SCA) variance, indicating predominance of additive and additive by additive gene effects in the inheritance for all studied traits. Cross P2xP3 significantly out yielded the best check (SC. 168) for the grain yield trait. The parental inbred lines, P1, P2 and P3, possessed significant desirable (GCA) effects for the grain yield trait. The cross combinations (P1xP5),(P2xP7), (P3xP7),(P4xP5),(P4xP6) and (P6xP7) showed significant positive sca effects for grain yield. Cross P2xP3 (10.32**) obtained superiority (%) relative to the check hybrid SC.168 for grain yield. On the other hand, five crosses viz. (P1xP3), (P1xP5), (P2xP3), (P2xP5) and (P3xP5) expressed positive superiority (%) over the check hybrid SC.3444 for same trait. Thus, these crosses could be recommended to be released as commercial hybrids by Maize Research Programme after further evaluation and testing in multi-locations in Egypt. [ABSTRACT FROM AUTHOR]

Human epidermal growth factor receptor 2 (ErbB2/HER2) is a tyrosine kinase receptor that is overexpressed in 25% of primary human breast cancers, as well as in multiple other cancers. HER2‐targeted therapies improved progression‐free and overall survival in patients with HER2+ breast cancers. However, associated resistance mechanisms and toxicity highlight the need for new therapeutic approaches for these cancers. We recently established that, in normal cells, HER2 is stabilized in a catalytically repressed state by direct interaction with members of the ezrin/radixin/moesin (ERM) family. In HER2‐overexpressing tumors, the low expression of moesin contributes to the aberrant activation of HER2. Through a screen designed to find moesin‐mimicking compounds, we identified ebselen oxide. We show that ebselen oxide, and some derivatives, conferred an efficient allosteric inhibition of overexpressed HER2, as well as mutated and truncated oncogenic forms of HER2, which are resistant to current therapies. Ebselen oxide selectively inhibited anchorage‐dependent and ‐independent proliferation of HER2+ cancer cells and showed a significant benefit in combination with current anti‐HER2 therapeutic agents. Finally, ebselen oxide significantly blocked HER2+ breast tumor progression in vivo. Collectively, these data provide evidence that ebselen oxide is a newly identified allosteric inhibitor of HER2 to be considered for therapeutic intervention on HER2+ cancers. [ABSTRACT FROM AUTHOR]

Surgery, radiotherapy, and chemotherapy are essential treatment modalities to target cancer cells, but they frequently cause damage to the normal tissue, potentially leading to side effects. As proton beam radiotherapy (PBT) can precisely spare normal tissue, this therapeutic option is of increasing importance regarding (neo-)adjuvant and definitive anti-cancer therapies. Akin to photon-based radiotherapy, PBT is often combined with systemic treatment, such as doxorubicin (Dox). This study compares the cellular response of human microvascular endothelial cells (HMEC-1) following irradiation with photons (X) or protons (H) alone and also in combination with different sequences of Dox. The cellular survival, cell cycle, apoptosis, proliferation, viability, morphology, and migration were all investigated. Dox monotreatment had minor effects on all endpoints. Both radiation qualities alone and in combination with longer Dox schedules significantly reduced clonogenic survival and proliferation, increased the apoptotic cell fraction, induced a longer G2/M cell cycle arrest, and altered the cell morphology towards endothelial-to-mesenchymal-transition (EndoMT) processes. Radiation quality effects were seen for metabolic viability, proliferation, and motility of HMEC-1 cells. Additive effects were found for longer Dox schedules. Overall, similar effects were found for H/H-Dox and X/X-Dox. Significant alterations between the radiation qualities indicate different but not worse endothelial cell damage by H/H-Dox. [ABSTRACT FROM AUTHOR]

Estimates of combining ability are useful in determining the value of watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) lines in producing new hybrids with high yield and better quality. This research was conducted to estimate the general combining ability (GCA), specific combining ability (SCA), and heterosis for five inbred lines chosen for watermelon breeding at the University of Guilan, Iran. Analysis of variance indicated significant differences among hybrids for fruit weight and the number of lateral branches per plant. The GCA and SCA effects were significant for fruit weight and the number of lateral branches, which indicates the existence of both additive and dominance effects in controlling these two traits. The low narrow sense heritability indicated the smaller impact of additive gene action on fruit weight and the number of lateral branches in watermelon. However, broad sense heritability was high for fruit weight (0.69) and the number of lateral branches (8.88). The large gap between the broad sense heritability and narrow sense heritability indicated the importance of dominance effects in controlling the fruit weight and the number of lateral branches compared to additive effects. These results justify the development of hybrid varieties to exploit the dominance genetic effects in watermelon. The highest fruit weight and the number of lateral branches belonged to the S × Red cross. The highest SCA for the fruit weight was associated with the cross An × S. The cross S × Red had the highest SCA for the number of lateral branches. The high-parent heterosis was also higher in the cross S × Red for these traits. In conclusion, the hybrid S × Red may be proposed for the production of a hybrid variety in watermelon. However, crossing among various genotypes to produce new inbred lines seems necessary for the hybrid breeding program of watermelons in Iran. [ABSTRACT FROM AUTHOR]

Salinity is one of the major abiotic stress factors that limit the productivity of crops, including wheat, in many regions of the world. Therefore, the priority in wheat breeding, to ensure global food security, is developing varieties that are adapted to saline environments. In situ, evaluation of wheat genotypes can provide valuable information on the performance of different genotypes under natural saline conditions and can help to identify the most salt-tolerant genotypes. To ensure an accurate evaluation of the performance of twenty-seven wheat genotypes under different environments, the trial was conducted on two different soil types (solonetz and chernozem) in two growing seasons. AMMI analysis shows that the environmental factor had the largest share (55.15%) in the variation of grain yield, where soil type had a dominant effect. Genotypes Renesansa, Harmonija, and Bankut 1205 achieved a high grain yield on both soil types. However, among the mentioned genotypes, the genotype Harmonija showed the highest tolerance to salinity. A significant proportion of the genotype and environment interaction (GEI; 25.89%) shows that there is a change in the ranking of genotypes across environments. According to the AMMI1 biplot, the genotypes Renesansa and Harmonija were distinguished by high grain yield and high stability. The environment Chernozem 2015/2016 had the greatest contribution to the GEI and the highest grain yield, while Solonetz 2017/2018 was characterized by the highest stability and the lowest grain yield. According to the AMMI2 biplot, genotype Harmonija achieved high stability in unfavourable environmental conditions that characterized the environment Solonetz 2017/2018.

Abstract Background: To evaluate the additive effects of orthokeratology (OK) lenses and 0.01% atropine on slowing axial elongation in myopic children. Methods: A prospective, randomized, double-blinded, placebo-controlled trial was conducted over a 12-month period. Sixty children aged 8 to 12 years with spherical equivalent refraction from − 1.00 to -4.00 D who had been wearing OK lenses successfully for 2 months (as baseline) were randomly assigned in a 1:1 ratio to combination group (combination of OK lens and 0.01% atropine eye drops) and control group (combination of OK lens and placebo). The primary outcome was change in axial length, along with secondary outcomes including change in pupil diameter (PD) and accommodative amplitude (AMP) at 12 months (measured at 4-month intervals). Results: After 12 months, the overall axial elongation was 0.10 ± 0.14 mm and 0.20 ± 0.15 mm (p = 0.01) in the combination and control groups, respectively. The change in axial length in the two groups showed significant differences only in the first four months (median [Q1, Q3] (95% CI), -0.01 mm [-0.07, 0.05] (-0.06, 0.04) vs. 0.04 mm [0.00, 0.10] (0.02, 0.09); p = 0.04), but no difference thereafter. Multivariate linear regression analysis showed that the axial elongation was significantly slower in the combination group than in the control group (standard β = -0.10, p = 0.02). PD significantly increased by 0.45 mm [0.20, 0.68] at the 4th month visit (p 0.05). Conclusion: The combination therapy was more effective than the OK lens alone in slowing axial elongation after 12 months of treatment, and mainly in the first 4 months. Trial registration: The First Affiliated Hospital of Zhengzhou University, ChiCTR2000033904. Registered 16/06/2020, http://www.chictr.org.cn/login.aspx?referurl=%2flistbycreater.aspx

The study was designed to evaluate the growth performance of exotic dual-purpose chicken breed of Koekoek (K) and Kuroiler (Ku) crosses with an improved Horro (H) chicken under reciprocal mating to select potential crosses for development of a synthetic breed in Ethiopia. The experiment was carried out on seven genotypes, including three pure lines (i.e., Horro, Koekoek, Kuroiler) and their direct (K × H, Ku × H) and reciprocal crosses (H × K and H × Ku). A total of 446one-day-old chicks from the seven genotypes were randomly distributed between pens using a completely randomized design with three replications (experiment period?). Data on body weight at hatch (DO), 4, 8, 12, 16 and 20 weeks of age, body weight gain, feed conversion rate (FCR) and mortality rate were calculated during the experiment. The crossbreeding effects were estimated to select the best genotype as a dam or a sire line in the synthetic breed for the next generation. The result showed that the highest mean body weight (P < 0.05) and body weight gain were related to Horro×Kuroiler crossbred chicken that followed by Ku×H, and pure Ku at most of studied ages. Average feed intake was comparable among genotypes. Additive (Ae), maternal and heterosis effects for body weight were significantly (P < 0.05) positive at most ages for both crosses. Additive effects range from 1.79 to 10.1% for Horro-Koekoek crosses, whereas it ranges from 1.6 to 44% for Horro-Kuroiler crosses. In Horro-Koekoek crosses, the highest positive contribution of maternal effect was observed for body weight at eight weeks (8.22 %). Estimates of heterosis effects (He) were positive and ranged from 6.54 to 13.79%. In the Horro-Kuroiler crosses, estimates of maternal additive (Me) and the heterosis effects on body weight were positive and significant (P < 0.05) at 8- and 12-weeks of age. Generally, the positive and significant additive effects suggest merits of the sire line in the growth performance which favors use of Kuroiler and Koekoek as sire lines to improve the body. Positive values and significant contribution of heterosis indicate the substantial effect of crossbreeding on body weight at most of the studied ages. From this study it can be recommended that crossbred hens sired by improved Horro (H × Ku) can be used for growth performance potential in the forthcoming synthetic breed development program.

Introduction: Characterization of germplasm collections for the wheat leaf rust gene Lr34 previously defined five haplotypes in spring wheat. All resistant lines had a 3-bp TTC deletion (null) in exon 11, resulting in the absence of a phenylalanine residue in the ABC transporter, as well as a single nucleotide C (Tyrosine in Lr34+) to T (Histidine in Lr34-) transition in exon 12. A rare haplotype present in Odesskaja 13 and Koktunkulskaja 332, both of intermediate rust resistance, had the 3-bp deletion typical of Lr34+ in exon 11 but the T nucleotide of Lr34- in exon 12. Methods: To quantify the role of each mutation in leaf rust resistance, Odesskaja 13 and Koktunkulskaja 332 were crossed to Thatcher and its near-isogenic line Thatcher-Lr34 (RL6058). Single seed descent populations were generated and evaluated for rust resistance in six different rust nurseries. Results: The Odesskaja 13 progeny with the TTC/T haplotype were susceptible with an average severity rating of 62.3%, the null/T haplotype progeny averaged 39.7% and the null/C haplotype was highly resistant, averaging 13.3% severity. The numbers for the Koktunkulskaja 332 crosses were similar with 63.5%, 43.5% and 23.7% severity ratings, respectively. Differences between all classes in all crosses were statistically significant, indicating that both mutations are independently additive for leaf rust resistance. The three-dimensional structural models of LR34 were used to analyze the locations and putative interference of both amino acids with the transport channel. Koktunkulskaja 332 also segregated for marker csLV46 which is linked to Lr46. Rust severity in lines with Lr34+ and csLV46+ had significantly lower rust severity ratings than those without, indicating the additivity of the two loci. Discussion: This has implications for the deployment of Lr34 in wheat cultivars and for the basic understanding of this important wheat multi-pest durable resistance gene. [ABSTRACT FROM AUTHOR]

We examined the response of Eurasian watermilfoil (Myriophyllum spicatum) coverage on Manistee Lake, Michigan (U.S.A.) in the presence of milfoil weevils (Euhrychiopsis lecontei). Among 150 sites, milfoil presence declined from 2008 levels of 34 (23%) sites to 2 (1%) sites by 2015 coincident with cumulative stocking of 259,500 weevils from 2007 to 2014. Severe winter temperatures also were associated with milfoil declines. Each 1°C decline in average low temperature during the preceding winter was associated with 3.4 (95% CI 0.8–6.1) fewer sites with milfoil. Impacts of weevil herbivory on watermilfoil may be accentuated by severe winter temperatures. Lake managers should, when possible, integrate weather conditions with weevil stocking regimes to control Eurasian watermilfoil. [ABSTRACT FROM AUTHOR]

Changes in environmental factors, such as temperature and UV, have significant impacts on the growth and development of both native and invasive plant species. However, few studies examine the combined effects of warming and enhanced UV on plant growth and performance in invasive species. Here, we investigated single and combined effects of warming and UV radiation on growth, leaf functional and photosynthesis traits, and nutrient content (i.e., total organic carbon, nitrogen and phosphorous) of invasive Solidago canadensis and its co-occurring native species, Artemisia argyi, when grown in culture racks in the greenhouse. The species were grown in monoculture and together in a mixed community, with and without warming, and with and without increased UV in a full factorial design. We found that growth in S. canadensis and A. argyi were inhibited and more affected by warming than UV-B radiation. Additionally, there were both antagonistic and synergistic interactions between warming and UV-B on growth and performance in both species. Overall, our results suggested that S. canadensis was more tolerant to elevated temperatures and high UV radiation compared to the native species. Therefore, substantial increases in temperature and UV-B may favour invasive S. canadensis over native A. argyi. Research focusing on the effects of a wider range of temperatures and UV levels is required to improve our understanding of the responses of these two species to greater environmental variability and the impacts of climate change. [ABSTRACT FROM AUTHOR]

IntroductionCharacterization of germplasm collections for the wheat leaf rust gene Lr34 previously defined five haplotypes in spring wheat. All resistant lines had a 3-bp TTC deletion (null) in exon 11, resulting in the absence of a phenylalanine residue in the ABC transporter, as well as a single nucleotide C (Tyrosine in Lr34+) to T (Histidine in Lr34-) transition in exon 12. A rare haplotype present in Odesskaja 13 and Koktunkulskaja 332, both of intermediate rust resistance, had the 3-bp deletion typical of Lr34+ in exon 11 but the T nucleotide of Lr34- in exon 12.MethodsTo quantify the role of each mutation in leaf rust resistance, Odesskaja 13 and Koktunkulskaja 332 were crossed to Thatcher and its near-isogenic line Thatcher-Lr34 (RL6058). Single seed descent populations were generated and evaluated for rust resistance in six different rust nurseries.ResultsThe Odesskaja 13 progeny with the TTC/T haplotype were susceptible with an average severity rating of 62.3%, the null/T haplotype progeny averaged 39.7% and the null/C haplotype was highly resistant, averaging 13.3% severity. The numbers for the Koktunkulskaja 332 crosses were similar with 63.5%, 43.5% and 23.7% severity ratings, respectively. Differences between all classes in all crosses were statistically significant, indicating that both mutations are independently additive for leaf rust resistance. The three-dimensional structural models of LR34 were used to analyze the locations and putative interference of both amino acids with the transport channel. Koktunkulskaja 332 also segregated for marker csLV46 which is linked to Lr46. Rust severity in lines with Lr34+ and csLV46+ had significantly lower rust severity ratings than those without, indicating the additivity of the two loci.DiscussionThis has implications for the deployment of Lr34 in wheat cultivars and for the basic understanding of this important wheat multi-pest durable resistance gene.

The present study investigated the additive effects of L-Arginine (L-Arg) with potassium carbonate (PC) on the quality characteristics of phosphate-free frankfurters. The results showed that L-Arg combined with PC could act as a viable phosphate replacer by decreasing cooking loss and improving the textural properties of phosphate-free frankfurters (p < 0.05), mainly because of its pH-raising ability. Moreover, L-Arg could assist PC in effectively retarding lipid oxidation in phosphate-free frankfurters during storage (p < 0.05). Furthermore, 0.1% L-Arg combined with 0.15% PC was found to exhibit the best optimal phosphate-replacing effect. This combination could also overcome quality defects and promote the sensory attributes of phosphate-free frankfurters to the maximum extent. Therefore, our results suggest that L-Arg combined with PC can be considered a feasible alternative for the processing of phosphate-free frankfurters with an improved quality profile and superior health benefits. [ABSTRACT FROM AUTHOR]

Drought and local habitat alteration are major environmental stressors shaping the aquatic biota in dryland rivers. However, the combined effects of these factors on aquatic biodiversity remain poorly understood. We collected macroinvertebrate data from Central Asian dryland rivers in Xinjiang, China, from 2012 to 2022, to investigate the individual and interactive effects of drought (as indicated by increasing values of Aridity, AI) and local habitat conditions (fine sediments, velocity and pH) on aquatic macroinvertebrate functional trait composition and diversity. We found that interactions of the selected environmental stressors exhibited more frequent additive than synergistic or antagonistic effects, leading to shifts in macroinvertebrate functional trait composition and diversity accordingly. Interaction of AI and fine sediments showed more pronounced synergistic effects (positive or negative) compared to others and had positive influences on traits like small body size, ovoviviparity, etc. Functional diversity metrics responded differently to stressor interactions, with FRic and FDis being negatively affected, whereas FEve was positively correlated to stressor interaction, suggesting the complementary roles of functional diversity metrics to diagnose impacts of stressor interactions. Overall, our study provides new insights into macroinvertebrate assemblage-stressor relationships in dryland rivers and can help better assess, predict and manage aquatic biodiversity in these rivers under ongoing environmental change., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Jensen's inequality predicts that the response of any given system to average constant conditions is different from its average response to varying ones. Environmental fluctuations in abiotic conditions are pervasive on Earth; yet until recently, most ecological research has addressed the effects of multiple environmental drivers by assuming constant conditions. One could thus expect to find significant deviations in the magnitude of their effects on ecosystems when environmental fluctuations are considered. Drawing on experimental studies published during the last 30 years reporting more than 950 response ratios (n = 5,700), we present a comprehensive analysis of the role that environmental fluctuations play across the tree of life. In contrast to the predominance of interactive effects of global-change drivers reported in the literature, our results show that their cumulative effects were additive (58%), synergistic (26%), and antagonistic (16%) when environmental fluctuations were present. However, the dominant type of interaction varied by trophic level (autotrophs: interactive; heterotrophs: additive) and phylogenetic group (additive in Animalia; additive and positive antagonism in Chromista; negative antagonism and synergism in Plantae). In addition, we identify the need to tackle how complex communities respond to fluctuating environments, widening the phylogenetic and biogeographic ranges considered, and to consider other drivers beyond warming and acidification as well as longer timescales. Environmental fluctuations must be taken into account in experimental and modeling studies as well as conservation plans to better predict the nature, magnitude, and direction of the impacts of global change on organisms and ecosystems. [ABSTRACT FROM AUTHOR]

The study was designed to evaluate the growth performance of exotic dual-purpose chicken breed of Koekoek (K) and Kuroiler (Ku) crosses with an improved Horro (H) chicken under reciprocal mating to select potential crosses for development of a synthetic breed in Ethiopia. The experiment was carried out on seven genotypes, including three pure lines (i.e., Horro, Koekoek, Kuroiler) and their direct (K x H, Ku x H) and reciprocal crosses (H x K and H x Ku). A total of 446one-day-old chicks from the seven genotypes were randomly distributed between pens using a completely randomized design with three replications (experiment period?). Data on body weight at hatch (DO), 4, 8, 12, 16 and 20 weeks of age, body weight gain, feed conversion rate (FCR) and mortality rate were calculated during the experiment. The crossbreeding effects were estimated to select the best genotype as a dam or a sire line in the synthetic breed for the next generation. The result showed that the highest mean body weight (P < 0.05) and body weight gain were related to HorroxKuroiler crossbred chicken that followed by KuxH, and pure Ku at most of studied ages. Average feed intake was comparable among genotypes. Additive (Ae), maternal and heterosis effects for body weight were significantly (P < 0.05) positive at most ages for both crosses. Additive effects range from 1.79 to 10.1% for Horro-Koekoek crosses, whereas it ranges from 1.6 to 44% for Horro-Kuroiler crosses. In Horro-Koekoek crosses, the highest positive contribution of maternal effect was observed for body weight at eight weeks (8.22 %). Estimates of heterosis effects (He) were positive and ranged from 6.54 to 13.79%. In the Horro-Kuroiler crosses, estimates of maternal additive (Me) and the heterosis effects on body weight were positive and significant (P < 0.05) at 8- and 12-weeks of age. Generally, the positive and significant additive effects suggest merits of the sire line in the growth performance which favors use of Kuroiler and Koekoek as sire lines to improve the body. Positive values and significant contribution of heterosis indicate the substantial effect of crossbreeding on body weight at most of the studied ages. From this study it can be recommended that crossbred hens sired by improved Horro (H x Ku) can be used for growth performance potential in the forthcoming synthetic breed development program. [ABSTRACT FROM AUTHOR]

Mendel (1866) suggested that if many heritable "factors" contribute to a trait, near-continuous variation could result. Fisher (1918) clarified the connection between Mendelian inheritance and continuous trait variation by assuming many loci, each with small effect, and by informally invoking the central limit theorem. Barton et al. (2017) rigorously analyze the approach to a multivariate Gaussian distribution of the genetic effects for descendants of parents who may be related. This commentary distinguishes three nested approximations, referred to as "infinitesimal genetics," "Gaussian descendants" and "Gaussian population," each plausibly called "the infinitesimal model." The first and most basic is Fisher's "infinitesimal" approximation of the underlying genetics - namely, many loci, each making a small contribution to the total variance. As Barton et al. (2017) show, in the limit as the number of loci increases (with enough additivity), the distribution of genotypic values for descendants approaches a multivariate Gaussian, whose variance-covariance structure depends only on the relatedness, not the phenotypes, of the parents (or whether their population experiences selection or other processes such as mutation and migration). Barton et al. (2017) call this rigorously defensible "Gaussian descendants" approximation "the infinitesimal model." However, it is widely assumed that Fisher's genetic assumptions yield another Gaussian approximation, in which the distribution of breeding values in a population follows a Gaussian - even if the population is subject to non-Gaussian selection. This third "Gaussian population" approximation, is also described as the "infinitesimal model." Unlike the "Gaussian descendants" approximation, this third approximation cannot be rigorously justified, except in a weak-selection limit, even for a purely additive model. Nevertheless, it underlies the two most widely used descriptions of selection-induced changes in trait means and genetic variances, the "breeder's equation" and the "Bulmer effect." Future generations may understand why the "infinitesimal model" provides such useful approximations in the face of epistasis, linkage, linkage disequilibrium and strong selection.

Mendel (1866) suggested that if many heritable "factors" contribute to a trait, near-continuous variation could result. Fisher (1918) clarified the connection between Mendelian inheritance and continuous trait variation by assuming many loci, each with small effect, and by informally invoking the central limit theorem. Barton et al. (2017) rigorously analyze the approach to a multivariate Gaussian distribution of the genetic effects for descendants of parents who may be related. This commentary distinguishes three nested approximations, referred to as "infinitesimal genetics," "Gaussian descendants" and "Gaussian population," each plausibly called "the infinitesimal model." The first and most basic is Fisher's "infinitesimal" approximation of the underlying genetics - namely, many loci, each making a small contribution to the total variance. As Barton et al. (2017) show, in the limit as the number of loci increases (with enough additivity), the distribution of genotypic values for descendants approaches a multivariate Gaussian, whose variance-covariance structure depends only on the relatedness, not the phenotypes, of the parents (or whether their population experiences selection or other processes such as mutation and migration). Barton et al. (2017) call this rigorously defensible "Gaussian descendants" approximation "the infinitesimal model." However, it is widely assumed that Fisher's genetic assumptions yield another Gaussian approximation, in which the distribution of breeding values in a population follows a Gaussian - even if the population is subject to non-Gaussian selection. This third "Gaussian population" approximation, is also described as the "infinitesimal model." Unlike the "Gaussian descendants" approximation, this third approximation cannot be rigorously justified, except in a weak-selection limit, even for a purely additive model. Nevertheless, it underlies the two most widely used descriptions of selection-induced changes in trait means and genetic variances, the "breeder's equation" and the "Bulmer effect." Future generations may understand why the "infinitesimal model" provides such useful approximations in the face of epistasis, linkage, linkage disequilibrium and strong selection.

Aflatoxin B1 (AFB1) is a common crop contaminant, while aflatoxin M1 (AFM1) is implicated in milk safety. Humans are likely to be simultaneously exposed to AFB1 and AFM1; however, studies on the combined interactive effects of AFB1 and AFM1 are lacking. To fill this knowledge gap, transcriptomic, proteomic, and microRNA (miRNA)-sequencing approaches were used to investigate the toxic mechanisms underpinning combined AFB1 and AFM1 actions in vitro. Exposure to AFB1 (1.25–20 μM) and AFM1 (5–20 μM) for 48 h significantly decreased cell viability in the intestinal cell line, NCM460. Multi-omics analyses demonstrated that additive toxic effects were induced by combined AFB1 (2.5 μM) and AFM1 (2.5 μM) in NCM460 cells and were associated with p53 signaling pathway, a common pathway enriched by differentially expressed mRNAs/proteins/miRNAs. Specifically, based on p53 signaling, cross-omics showed that AFB1 and AFM1 reduced NCM460 cell viability via the hsa-miR-628-3p- and hsa-miR-217-5p-mediated regulation of cell surface death receptor (FAS), and also the hsa-miR-11-y-mediated regulation of cyclin dependent kinase 2 (CDK2). We provide new insights on biomarkers which reflect the cytotoxic effects of combined AFB1 and AFM1 toxicity. [ABSTRACT FROM AUTHOR]

Grain size is an important component of quality and harvest traits in the field of rice breeding. Although numerous quantitative trait loci (QTLs) of grain size in rice have been reported, the molecular mechanisms of these QTLs remain poorly understood, and further research on QTL observation and candidate gene identification is warranted. In our research, we developed a suite of F2 intercross populations from a cross of 9311 and CG. These primary populations were used to map QTLs conferring grain size, evaluated across three environments, and then subjected to bulked-segregant analysis-seq (BSA-seq). In total, 4, 11, 12 and 14 QTLs for grain length (GL), grain width (GW), 1000-grain weight (TGW), and length/width ratio (LWR), respectively, were detected on the basis of a single-environment analysis. In particular, over 200 splicing-related sites were identified by whole-genome sequencing, including one splicing-site mutation with G>A at the beginning of intron 4 on Os03g0841800 (qGL3.3), producing a smaller open reading frame, without the third and fourth exons. A previous study revealed that the loss-of-function allele caused by this splicing site can negatively regulate rice grain length. Furthermore, qTGW2.1 and qGW2.3 were new QTLs for grain width. We used the near-isogenic lines (NILs) of these GW QTLs to study their genetic effects on individuals and pyramiding, and found that they have additive effects on GW. In summary, these discoveries provide a valuable genetic resource, which will facilitate further study of the genetic polymorphism of new rice varieties in rice breeding. [ABSTRACT FROM AUTHOR]

The interacting effects of drought and fire on ecological communities are poorly understood. Long-term studies in the Warrumbungle Mountains, central-west New South Wales, subject to drought and fire during the past 21 years, enabled their separate and combined effects to be quantified for individual species and functional groups. Insectivores (especially ground-foragers) dominated previous lists of declining species in this region of NSW and were also prominent in the present work. Insectivores were more likely to be drought- than fire-affected, with seven species declining due to drought, three to drought plus fire, and three to fire alone. Our analyses also revealed declines in a suite of honeyeaters (Meliphagidae), previously not reported as declining. Honeyeaters are major pollinators of eucalypts, so the loss of nectarivores and insectivores has far-reaching implications for pollination, recruitment, successional dynamics, and forest health. Four honeyeater species were adversely affected by drought, five by fire, and one by a combination of drought and fire. Drought and fire, alone or in combination, were implicated in declines of granivores, including the Crimson Rosella Platycercus elegans, and frugivores, especially the Mistletoebird Dicaeum hirundinaceum, the latter reflecting the loss of mistletoes in fire-affected landscapes, and foreshadowing additional losses due to the reliance on mistletoe by many species. Another group not previously identified as threatened, but declining due to drought were two omnivores, the Pied Currawong Strepera graculina and Australian Raven Corvus coronoides. Hollow-nesting birds including two species of treecreeper and the Laughing Kookaburra Dacelo novaeguineae fared badly. Several common Australian species were among the decliners, including Laughing Kookaburra, Willie Wagtail Rhipidura leucophrys and Australian Magpie Cracticus tibicen. We conclude that no suite of birds is exempt from these environmental stressors, and predict that, as droughts reduce populations at regional scales and fires diminish carrying capacity of critical habitats at landscape scales, rarer species will decline to local extinction while more commonly observed species will be reduced in abundance. [ABSTRACT FROM AUTHOR]

Early flowering facilitates crops to adapt multiple cropping systems or growing regions with a short frost-free season; however, it usually brings an obvious yield loss. In this study, we identified that the three genes, namely, BnFLC.A2, BnFLC.C2, and BnFLC.A3b, are the major determinants for the flowering time (FT) variation of two elite rapeseed (Brassica napus L.) accessions, i.e., 616A and R11. The early-flowering alleles (i.e., Bnflc.a2 and Bnflc.c2) and late-flowering allele (i.e., BnFLC.A3b) from R11 were introgressed into the recipient parent 616A through a breeding strategy of marker-assisted backcross, giving rise to eight homozygous near-isogenic lines (NILs) associated with these three loci and 19 NIL hybrids produced by the mutual crossing of these NILs. Phenotypic investigations showed that NILs displayed significant variations in both FT and plant yield (PY). Notably, genetic analysis indicated that BnFLC.A2, BnFLC.C2, and BnFLC.A3b have additive effects of 1.446, 1.365, and 1.361 g on PY, respectively, while their dominant effects reached 3.504, 2.991, and 3.284 g, respectively, indicating that the yield loss caused by early flowering can be successfully compensated by exploring the heterosis of FT genes in the hybrid NILs. Moreover, we further validated that the heterosis of FT genes in PY was also effective in nonNIL hybrids. The results demonstrate that the exploration of the potential heterosis underlying the FT genes can coordinate early flowering (maturation) and high yield in rapeseed (B. napus L.), providing an effective strategy for early flowering breeding in crops. [ABSTRACT FROM AUTHOR]

Genomic selection uses whole-genome marker models to predict phenotypes or genetic values for complex traits. Some of these models fit interaction terms between markers, and are therefore called epistatic. The biological interpretation of the corresponding fitted effects is not straightforward and there is the threat of overinterpreting their functional meaning. Here we show that the predictive ability of epistatic models relative to additive models can change with the density of the marker panel. In more detail, we show that for publicly available Arabidopsis and rice datasets, an initial superiority of epistatic models over additive models, which can be observed at a lower marker density, vanishes when the number of markers increases. We relate these observations to earlier results reported in the context of association studies which showed that detecting statistical epistatic effects may not only be related to interactions in the underlying genetic architecture, but also to incomplete linkage disequilibrium at low marker density (“Phantom Epistasis”). Finally, we illustrate in a simulation study that due to phantom epistasis, epistatic models may also predict the genetic value of an underlying purely additive genetic architecture better than additive models, when the marker density is low. Our observations can encourage the use of genomic epistatic models with low density panels, and discourage their biological over-interpretation.

ABSTRACT The objective of this study was to estimate the varietal and heterotic effects related to grain yield, popping expansion and resistance of popcorn against fall armyworm. Twelve open-pollinated varieties were intercrossed in a complete diallel mating scheme. The 66 experimental hybrids, together with their parents and controls Zapalote Chico, BRS 1030 and IAC 125, resulted in a total of 81 treatments, evaluated in an experiment in a 9x9 triple-square lattice design, in Maringá-PR and Araruna-PR. The data were analyzed according to the diallel model of Gardner and Eberhart (1966). The following traits were evaluated: grain yield (GR); mean plant height (PH) and insertion height of the highest ear in the stem (EI); silking (SIL); final plant density (FD) and popping expansion (PE). Variety PR 023 is indicated for intrapopulation improvement for grain yield. Regarding popping expansion, the results indicated cross SAM x UNB 2U C5 for recurrent reciprocal selection. In relation to resistance to fall armyworm, the varieties PARA 172, PA 091, PR 023, and SE 013 were selected for intrapopulation improvement. On the other hand, the crosses PARA 172 x BOZM 260, PA 091 x BOYA 462 and SAM x UNB 2U C5 can be indicated for interpopulation improvement.

The influence of biuret, a degradation impurity, on the crystallisability, nucleation, growth and morphology of urea, is examined as a function of supersaturation in ethanolic solutions, through a combination of polythermal turbidometry assessment and optical microscopy measurements, complemented by molecular modelling. The inclusion of a small amount of impurity is found to increase the stability of supersaturated solutions through widening the metastable zone width. Polythermal analysis of crystallisation and dissolution on‐sets as a function of cooling and heating rate, respectively, is found to be concentration‐dependent associated with both the pure and doped systems, with the nucleation mechanism changing from progressive to instantaneous with increasing solute concentration. The addition of biuret significantly increases nucleation rate when compared to the pure system, from 9.2–20.5 to 9.3–67.7 nm−3 s−1, along with a decrease in the critical nucleus size, as would be consistent with the impurity promoting nucleation i. e. resulting in more nuclei and smaller crystals. The crystal habit of urea in ethanolic solutions is found to be dominated by the prismatic {110} faces, and smaller polar {111} capping faces. The mean crystal growth rates of these faces are found to increase with respect to supersaturation, with the {111} faces exhibiting a greater level of increase than {110}, consistent with stronger solvent binding which restricts the growth of the {110} face. The addition of biuret is found to have a greater effect on retarding the growth of {111} compared to {110}, resulting in a more compact and equant morphology. These results are rationalised with computational visualisation and modelling of the surface chemistry of the crystal habit planes together with the energetics of the impurity binding to them. [ABSTRACT FROM AUTHOR]

Changes in environmental factors, such as temperature and UV, have significant impacts on the growth and development of both native and invasive plant species. However, few studies examine the combined effects of warming and enhanced UV on plant growth and performance in invasive species. Here, we investigated single and combined effects of warming and UV radiation on growth, leaf functional and photosynthesis traits, and nutrient content (i.e., total organic carbon, nitrogen and phosphorous) of invasive Solidago canadensis and its co-occurring native species, Artemisia argyi, when grown in culture racks in the greenhouse. The species were grown in monoculture and together in a mixed community, with and without warming, and with and without increased UV in a full factorial design. We found that growth in S. canadensis and A. argyi were inhibited and more affected by warming than UV-B radiation. Additionally, there were both antagonistic and synergistic interactions between warming and UV-B on growth and performance in both species. Overall, our results suggested that S. canadensis was more tolerant to elevated temperatures and high UV radiation compared to the native species. Therefore, substantial increases in temperature and UV-B may favour invasive S. canadensis over native A. argyi. Research focusing on the effects of a wider range of temperatures and UV levels is required to improve our understanding of the responses of these two species to greater environmental variability and the impacts of climate change.

The present study investigated the additive effects of L-Arginine (L-Arg) with potassium carbonate (PC) on the quality characteristics of phosphate-free frankfurters. The results showed that L-Arg combined with PC could act as a viable phosphate replacer by decreasing cooking loss and improving the textural properties of phosphate-free frankfurters (p < 0.05), mainly because of its pH-raising ability. Moreover, L-Arg could assist PC in effectively retarding lipid oxidation in phosphate-free frankfurters during storage (p < 0.05). Furthermore, 0.1% L-Arg combined with 0.15% PC was found to exhibit the best optimal phosphate-replacing effect. This combination could also overcome quality defects and promote the sensory attributes of phosphate-free frankfurters to the maximum extent. Therefore, our results suggest that L-Arg combined with PC can be considered a feasible alternative for the processing of phosphate-free frankfurters with an improved quality profile and superior health benefits.

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Primary‐amine thioureas are preeminent organocatalysts used in challenging asymmetric Michael‐type reactions, such as in the addition of nitromethane to enones. However, the complete reaction mechanism of this reaction has not yet been fully elucidated. Considering that the primary‐amine group could act via both base and iminium ion catalysis, both mechanisms were investigated in this work using theoretical methods. The calculations have indicated that the base catalysis is kinetically unfeasible, with very high overall ΔG≠. An imine‐iminium ion catalysis is a viable route. This mechanism requires the participation of an acid as a cocatalyst (additive) in several steps, including the formation of the imine. The rate‐determining step corresponds to the proton transfer from the nitromethane to the imine, forming the nitronate‐iminium ion‐pair intermediate. This complex reacts via nucleophilic attack of the nitronate ion to the electrophilic carbon of the iminium ion, forming a new C−C bond that determines the enantioselectivity of the reaction. [ABSTRACT FROM AUTHOR]

Aflatoxin B1 (AFB1) is a common crop contaminant, while aflatoxin M1 (AFM1) is implicated in milk safety. Humans are likely to be simultaneously exposed to AFB1 and AFM1; however, studies on the combined interactive effects of AFB1 and AFM1 are lacking. To fill this knowledge gap, transcriptomic, proteomic, and microRNA (miRNA)-sequencing approaches were used to investigate the toxic mechanisms underpinning combined AFB1 and AFM1 actions in vitro. Exposure to AFB1 (1.25–20 μM) and AFM1 (5–20 μM) for 48 h significantly decreased cell viability in the intestinal cell line, NCM460. Multi-omics analyses demonstrated that additive toxic effects were induced by combined AFB1 (2.5 μM) and AFM1 (2.5 μM) in NCM460 cells and were associated with p53 signaling pathway, a common pathway enriched by differentially expressed mRNAs/proteins/miRNAs. Specifically, based on p53 signaling, cross-omics showed that AFB1 and AFM1 reduced NCM460 cell viability via the hsa-miR-628-3p- and hsa-miR-217-5p-mediated regulation of cell surface death receptor (FAS), and also the hsa-miR-11-y-mediated regulation of cyclin dependent kinase 2 (CDK2). We provide new insights on biomarkers which reflect the cytotoxic effects of combined AFB1 and AFM1 toxicity.

Spike fertility index (SF) is a trait easily measured at maturity and strongly associated with the number of grains per unit area. In order to identify genomic regions involved in SF control, a biparental (Baguette 10 × Klein Chajá) population of 80 recombinant inbred lines (RIL) was used. Seven field trails were conducted to determine the SF BLUP value per RIL. RILs were genotyped using a commercial chip (Axiom® 35K SNP Wheat Breeder's Array, Affimetrix). A linkage map was constructed with 857 SNP markers, and SF QTL mapping was performed. The narrow-sense heritability of SF was 0.89. Three genomic regions (QTL) associated with SF were found on chromosomes 2D, 4A, and 7A. The proportion of genetic variation explained by these three QTL was 32%, with no significant epistatic interaction between QTL. [ABSTRACT FROM AUTHOR]

Dominant genetic effects may provide a critical contribution to the total genetic variation of quantitative and complex traits. However, investigations of genome-wide markers to study the genomic prediction (GP) and genetic mechanisms of complex traits generally ignore dominant genetic effects. The increasing availability of genomic datasets and the potential benefits of the inclusion of non-additive genetic effects in GP have recently renewed attention to incorporation of these effects in genomic prediction models. In the present study, data from 498 genotyped Alpine Merino sheep were adopted to estimate the additive and dominant genetic effects of 9 wool and blood traits via two linear models: (1) an additive effect model (MAG) and (2) a model that included both additive and dominant genetic effects (MADG). Moreover, a method of 5-fold cross validation was used to evaluate the capability of GP in the two different models. The results of variance component estimates for each trait suggested that for fleece extension rate (73%), red blood cell count (28%), and hematocrit (25%), a large component of phenotypic variation was explained by dominant genetic effects. The results of cross validation demonstrated that the MADG model, comprising additive and dominant genetic effects, did not display an apparent advantage over the MAG model that included only additive genetic effects, i.e., the model that included dominant genetic effects did not improve the capability for prediction of the genomic model. Consequently, inclusion of dominant effects in the GP model may not be beneficial for wool and blood traits in the population of Alpine Merino sheep.

Abstract: The objective of this work was to identify corn (Zea mays) genotypes with forage potential and to evaluate the efficiency of testers to discriminate forage traits in topcrosses, considering the contribution of additive and nonadditive genes. The experiment was carried out in the 2015/2016 and 2016/2017 crop seasons, in a randomized complete block design with three replicates. Thirty S3 corn progenies were evaluated in topcrosses with the AG8025, P30B39, MLP102, 60.H23.1, and 70.H26.1 testers. The following traits were assessed: forage dry mass yield, neutral detergent fiber, acid detergent fiber, and forage dry mass degradability. Progenies 205.2, 159.6, and 199.2, in this order, presented the best performance for forage potential. Testers 60.H23.1 and 70.H26.1 better expressed the genetic variability between progenies. For all traits in both crop seasons, there is a predominance of the action of genes of nonadditive effects.

Although self-control consistently emerges as one of the most robust correlates of delinquent behavior, limited empirical attempts have been made to explore the contextual variability of the relationship between self-control and delinquency outside of Western societies. Using data collected from 587 seventh- to ninth-grade students across 10 middle schools in a rural county of Southeast China, we examine self-control's efficacy in explaining juvenile delinquency in the presence of external environmental factors, and investigate relative strength of self-control and contextual factors in predicting delinquent behaviors. Our results confirm that self-control is an important predictor of delinquent behavior in a non-Western cultural context. However, certain environmental factors rooted in family, school, and peer groups are also shown to be the predictors of delinquent behavior where strength seems to exceed that of self-control. These findings shed more nuanced insights on the nexus between self-control, external situations, and delinquency, and in a broader sense, contribute to the elaboration of a more comprehensive understanding of self-control theory. [ABSTRACT FROM AUTHOR]

Genomic selection uses whole-genome marker models to predict phenotypes or genetic values for complex traits. Some of these models fit interaction terms between markers, and are therefore called epistatic. The biological interpretation of the corresponding fitted effects is not straightforward and there is the threat of overinterpreting their functional meaning. Here we show that the predictive ability of epistatic models relative to additive models can change with the density of the marker panel. In more detail, we show that for publicly available Arabidopsis and rice datasets, an initial superiority of epistatic models over additive models, which can be observed at a lower marker density, vanishes when the number of markers increases. We relate these observations to earlier results reported in the context of association studies which showed that detecting statistical epistatic effects may not only be related to interactions in the underlying genetic architecture, but also to incomplete linkage disequilibrium at low marker density (“Phantom Epistasis”). Finally, we illustrate in a simulation study that due to phantom epistasis, epistatic models may also predict the genetic value of an underlying purely additive genetic architecture better than additive models, when the marker density is low. Our observations can encourage the use of genomic epistatic models with low density panels, and discourage their biological over-interpretation. [ABSTRACT FROM AUTHOR]

The objective of this study was to estimate the varietal and heterotic effects related to grain yield, popping expansion and resistance of popcorn against fall armyworm. Twelve open-pollinated varieties were intercrossed in a complete diallel mating scheme. The 66 experimental hybrids, together with their parents and controls Zapalote Chico, BRS 1030 and IAC 125, resulted in a total of 81 treatments, evaluated in an experiment in a 9x9 triple-square lattice design, in Maringá-PR and Araruna-PR. The data were analyzed according to the diallel model of Gardner and Eberhart (1966). The following traits were evaluated: grain yield (GR); mean plant height (PH) and insertion height of the highest ear in the stem (EI); silking (SIL); final plant density (FD) and popping expansion (PE). Variety PR 023 is indicated for intrapopulation improvement for grain yield. Regarding popping expansion, the results indicated cross SAM x UNB 2U C5 for recurrent reciprocal selection. In relation to resistance to fall armyworm, the varieties PARA 172, PA 091, PR 023, and SE 013 were selected for intrapopulation improvement. On the other hand, the crosses PARA 172 x BOZM 260, PA 091 x BOYA 462 and SAM x UNB 2U C5 can be indicated for interpopulation improvement. [ABSTRACT FROM AUTHOR]

• A new anti-HER2 humanized monoclonal antibody, 19H6-Hu, binds to new epitopes within the domain III and in proximity to the domain IV of HER2 ECD that are different from trastuzumab and pertuzumab. • Combination of 3 antibodies, 19H6-Hu, inetetamab (a trastuzumab analog) and pertuzumab exhibited much stronger inhibition of large NCI-N87 tumor xenografts (>400mm3) than the current standard of care, inetetamab (trastuzumab) plus Docetaxal (DTX), as well as the combination of 19H6-Hu, inetetamab and DTX. • The tumor volume was reduced by 50% by the triple antibody combination, indicating that the three antibodies in combination not only blocked the tumor growth, but also led to tumor shrinkage. • 19H6-Hu significantly enhanced the anti-tumor effects of trastuzumab on multiple xenograft tumor models, including HCC1954, a trastuzumab resistant cell line. • 19H6-Hu in combination with trastuzumab was more effective at blocking phosphorylation of ERK1/2, AKT(S473)and HER2 (Y1248) in HER2-positive cancer cells compared to trastuzumab alone or in combination with pertuzumab. The majority of patients with metastatic breast cancer who are treated with the anti-HER2 monoclonal antibody, trastuzumab, generally develop resistance to the drug within a year after initiation of the treatment. Here we describe a new anti-HER2 humanized monoclonal antibody, 19H6-Hu, which binds to HER2 extracellular domain (ECD) with high affinity and inhibits proliferation of multiple HER2-overexpressing cancer cell lines as a single agent or in combination with trastuzumab. 19H6-Hu binds to the domain III in proximity to the domain IV of HER2 ECD, which differs from trastuzumab and pertuzumab. 19H6-Hu in combination with trastuzumab was more effective at blocking phosphorylation of ERK1/2, AKT(S473)and HER2 (Y1248) in HER2-positive cancer cells compared to trastuzumab alone or in combination with pertuzumab. Combination of three antibodies, 19H6-Hu, inetetamab (a trastuzumab analog) and pertuzumab exhibited much stronger inhibition of large NCI-N87 tumor xenografts (>400mm3) than the current standard of care, inetetamab (trastuzumab) plus Docetaxel (DTX), as well as the combination of 19H6-Hu, inetetamab and DTX. Our results highlight the functional variability of HER2 domains and provide a new insight into the design of HER2-targeting agents. [ABSTRACT FROM AUTHOR]

Five ruminally cannulated heifers were used in an incomplete 6 × 6 Latin square design to determine the effects of cereal silage (barley vs. corn), cereal grain (barley vs. corn vs. a 50:50 blend of barley and corn), and their interaction (S × G) on dry matter intake, ruminal fermentation, total tract digestibility, nitrogen balance, and in situ degradation. Corn silage (CS) or barley silage (BS) was included at 8% of dietary dry matter (DM). Within each silage source, diets contained (DM basis) either dry-rolled barley (BG; 86%), dry-rolled corn (CG; 85%), or an equal blend of barley and corn (BLEND; 85%). Periods were 25 d, with 5 d of dietary transition, 13 d of dietary adaptation, and 7 d of data and sample collection. Samples collected included feed and refusals, total urine and feces, and ruminal fluid. All data were analyzed using the Mixed model of SAS with the fixed effects of silage, grain, and the S × G. Dry matter intake (P ≥ 0.19) and mean ruminal pH (P ≥ 0.096) were not affected by the silage, grain, or S × G. Total short-chain fatty acid concentrations were greater for BLEND than BG or CG (grain, P = 0.003) and for CS (silage, P = 0.009) relative to BS. The molar proportion of acetate was greater for BS-BG and BS-CG (S × G, P < 0.001), while molar proportion of propionate was greater for CS-BG (S × G, P < 0.001) relative to other silage and grain source combinations. Rumen ammonia-N concentration was greater for CG than BG, or BLEND (grain, P < 0.001), and greater for CS compared to BS (silage, P = 0.023). Apparent total tract digestibility of DM, organic matter, neutral detergent fiber, starch, and gross energy were greatest for BG (grain, P ≤ 0.035). Digestible energy content (Mcal/kg) was greater for BG (grain, P = 0.029) than CG and BLEND. Total nitrogen retention (g/d and % of intake) was greatest for CS-BG (S × G, P ≤ 0.033) relative to all other treatments. In situ degradation rates of DM, crude protein, and starch were greater for BG than CG (P ≤ 0.004). The potentially degradable fraction of DM, crude protein, and starch was greater for CG (P ≤ 0.031), while the undegradable fraction was greater for BG (P ≤ 0.046). For silage sources, CS had greater 24 h in situ DM digestibility (P = 0.009) and starch digestibility (24, 48, and 72 h incubations, P ≤ 0.034) relative to BS. Results suggest that while feeding a combination of CS and BG promotes propionate production and greater N retention; few other additive effects were observed. [ABSTRACT FROM AUTHOR]