614 results on '"Adamo, S."'
Search Results
2. Hydroxytyrosol modulates the levels of microRNA-9 and its target sirtuin-1 thereby counteracting oxidative stress-induced chondrocyte death
- Author
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D'Adamo, S., Cetrullo, S., Guidotti, S., Borzì, R.M., and Flamigni, F.
- Published
- 2017
- Full Text
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3. The Specificity of Behavioral Fever in the Cricket Acheta domesticus
- Author
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Adamo, S. A.
- Published
- 1998
- Full Text
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4. Waterlandbouw : Kansen en keerzijden van natte teelten
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D’Adamo, S., Barbosa, M., Braak, K. van der, Geurts, J., Janssen, M., Meer, J. van der, Murk, T., Nauta, R., Osinga, R., D’Adamo, S., Barbosa, M., Braak, K. van der, Geurts, J., Janssen, M., Meer, J. van der, Murk, T., Nauta, R., and Osinga, R.
- Abstract
De laatste jaren is er duidelijk een kentering gekomen in de manier waarop we naar water als bron van voedsel kijken. Water blijkt namelijk ook kansen te bieden om nieuwe duurzame plantaardige producten te kweken, CO2 te binden, bedreigde vissoorten te ontzien, en voedsel te kweken met minimale vervuiling en impact op de natuur. De aanleg van windparken in zee bieden ongekende mogelijkheden om in afgesloten gebieden nieuwe vormen van duurzame ‘aquacultuur’ te verkennen. Over al die experimenten en vormen van duurzame (en minder duurzame) kweek gaat dit biocahier. De rode draad is duurzaamheid: het zo efficiënt mogelijk gebruiken van grondstoffen, of het zo efficiënt mogelijk produceren van voedsel met minimale impact voor het milieu.
- Published
- 2023
5. Hypes en hoop voor micro-algen als grondstofleverancier
- Author
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Wijffels, R.H., Janssen, M.G.J., D'Adamo, S., Südfeld, C.A.K.B., Barbosa, M.J., Wijffels, R.H., Janssen, M.G.J., D'Adamo, S., Südfeld, C.A.K.B., and Barbosa, M.J.
- Abstract
Micro-algen kunnen functioneren als grondstof voor voedsel, energie enchemicaliën. Ze groeien sneller en produceren meer per oppervlakte-eenheiddan gewassen. Bovendien concurreren ze niet met landbouwgrond en nemenalle nutriënten uit het water op.
- Published
- 2023
6. MicroRNA-155 suppresses autophagy in chondrocytes by modulating expression of autophagy proteins
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D'Adamo, S., Alvarez-Garcia, O., Muramatsu, Y., Flamigni, F., and Lotz, M.K.
- Published
- 2016
- Full Text
- View/download PDF
7. A simplified clinical risk score predicts the need for early endoscopy in non-variceal upper gastrointestinal bleeding
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Pallotta, L., Vitale, M.A., Parodi, M.C., Balzano, A., Sturniolo, G.C., Lamboglia, F., D’Angelo, A., Testai, F.V., Frosini, G., Marini, M., D’Amico, G., Montalbano, L.M., Sinagra, E., Macarri, G., Antonini, F., Occhigrossi, G., Caliendo, S., Campaioli, M., Peccianti, C., Spotti, D., Colucci, A., Marin, R., Cervellin, E., Conio, M., De Matthaeis, M., Biagini, M., Scarpulla, G., Camilleri, S., Lauri, A., De Fanis, C., Craxì, A., Peralta, S., Kohn, A., Tesi, A., Azzurro, M., Ferronato, A., Conigliaro, R., Manno, M., Manta, R., Adamo, S., Brunelli, E., Ridolfi, F., De Boni, M., De Bona, M., Guglielmi, F.W., Mazzuoli, S., Occhipinti, P., Ventrucci, M., Pozzato, P., Milani, S., Virgilio, C., Costa, G., Di Bella, S., Tammaro, Leonardo, Buda, Andrea, Di Paolo, Maria Carla, Zullo, Angelo, Hassan, Cesare, Riccio, Elisabetta, Vassallo, Roberto, Caserta, Luigi, Anderloni, Andrea, and Natali, Alessandro
- Published
- 2014
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8. What to Do, and What Not to Do, When Diagnosing and Treating Breakthrough Cancer Pain (BTcP): Expert Opinion
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Vellucci, R., Fanelli, G., Pannuti, R., Peruselli, C., Adamo, S., Alongi, G., Amato, F., Consoletti, L., Lamarca, L., Liguori, S., Lo Presti, C., Maione, A., Mameli, S., Marinangeli, F., Marulli, S., Minotti, V., Miotti, D., Montanari, L., Moruzzi, G., Palermo, S., Parolini, M., Poli, P., Tirelli, W., Valle, A., Romualdi, P., and Working Group Nientemale DEI
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- 2016
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9. At the Border: Reflections on Psychoanalytically Oriented Counselling in an Italian University Setting
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Adamo, S. M. G., Fontana, M. R., Preti, E., Prunas, A., Riffaldi, M. L., and Sarno, I.
- Abstract
This paper analyses the "border" position of counselling services in Italian universities. The concept of "border" has great relevance in the work of psychoanalytically oriented university students' counsellors. Counselling is part of a continuum of interventions, from befriending to psychotherapy. This closeness encompasses the need for identifying specificities and differences among interventions. Moreover, a university counselling service occupies a "marginal" position, continuously confronted with the risks of rejection and/or assimilation. The concept of "border" is also useful to define the developmental phase of university students, crossing the border between late adolescence and young adulthood, as represented by Conrad's novel "The shadow line" (1917/1999), recently analysed by Green (2008) as the metaphoric demarcation line between youth and adulthood. Finally, most of the students referring to our counselling service come from families that for the first time crossed the border of university education. (Contains 3 notes.)
- Published
- 2012
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10. Primary Anorectal Reconstruction after Abdominoperineal Excision by Coloperineal Pull-Through and Electrically Stimulated Graciloplasty: Efficiency and Limitations of a Hard-to-Catch-on Procedure
- Author
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Violi, Vincenzo, Boselli, Adamo S., Costi, Renato, Roncoroni, Luigi, and Farinon, Attilio Maria, editor
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- 2002
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11. The colors of CRISPR-Cas Type V : Broadening the genome editing toolkit for Saccharomyces cerevisiae
- Author
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van der Oost, J., D'Adamo, S., Adiego Pérez, Belén, van der Oost, J., D'Adamo, S., and Adiego Pérez, Belén
- Published
- 2022
12. A SynBio community comes of age: Political, academical, industrial, and societal developments in the Netherlands
- Author
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Bhatt, Darchak K., Crooijmans, Marjolein E., Coenradij, Jelmer, Maciá Valero, Alicia, Lubbers, Maarten, Asín García, E., Newdall, N.A., D'Adamo, S., Claassens, N.J., Billerbeck, Sonja, Bhatt, Darchak K., Crooijmans, Marjolein E., Coenradij, Jelmer, Maciá Valero, Alicia, Lubbers, Maarten, Asín García, E., Newdall, N.A., D'Adamo, S., Claassens, N.J., and Billerbeck, Sonja
- Abstract
Synthetic biology (SynBio) is a rapidly growing scientific discipline. In the Netherlands, various universities and companies are tackling a variety of opportunities and challenges within this field. In this perspective article, we review the current synthetic biology landscape in the Netherlands across academia, industry, politics, and society. Especially within Dutch academia there is an active, though only partially connected, research community involved in various domains of SynBio. Mostly supported by governmental funding, academic research is focusing on top-down synthetic biology, involving the engineering of, for example, bacteria and yeast for bioproduction, as well as bottom-up and cell-free synthetic biology aiming to understand life and build synthetic cells. There is also a large number of talented and motivated students interested in the field, exemplified by the participation and success of Dutch teams in the international iGEM synthetic biology competition. Commercial synthetic biology activities are taking place in various large industrial companies, as well as in start-ups and spin-offs, mostly divided over several ‘SynBio hubs’ in the Netherlands. However, the investment, regulatory and public-perception landscape is not yet optimal to stimulate entrepreneurial activities in SynBio. The Dutch and global society can further benefit from the large promise of SynBio through better integration of people active in the Dutch SynBio field, frequent political and public dialogue, and more attention towards regulatory issues. The recently founded Dutch synthetic biology association SynBioNL aims to contribute to realizing a positive impact on society by stimulating advances of the field in the Netherlands and beyond.
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- 2022
13. Closed-state inactivation of cardiac, skeletal, and neuronal sodium channels is isoform specific
- Author
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Brake, Niklas, primary, Mancino, Adamo S., additional, Yan, Yuhao, additional, Shimomura, Takushi, additional, Kubo, Yoshihiro, additional, Khadra, Anmar, additional, and Bowie, Derek, additional
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- 2022
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14. Epigenetics of Muscle Disorders
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Moresi, V., primary, Marroncelli, N., additional, Pigna, E., additional, and Adamo, S., additional
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- 2016
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15. List of Contributors
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Abdelfatah, E., primary, Adamo, S., additional, Ahuja, N., additional, Al Eissa, M., additional, Alenghat, T., additional, Altorok, N., additional, Altucci, L., additional, Antonello, Z.A., additional, Arasaradnam, R.P., additional, Ben-Aderet, L., additional, Bhalla, S., additional, Bitzer, M., additional, Bloch, W., additional, Burrowes, S.G., additional, Butt, N.A., additional, Cacabelos, R., additional, Chen, H., additional, Chen, P., additional, Cheng, B., additional, Chun, P., additional, Cox, O.H., additional, Deblois, G., additional, Dekker, F.J., additional, Dell'Aversana, C., additional, Dvir-Ginzberg, M., additional, Eissenberg, J.C., additional, Elayan, J., additional, Fincher, A.S., additional, Fischer, A., additional, Giorgio, C., additional, Gomes, M.V., additional, Greenwood-Van Meerveld, B., additional, Hall, J.G., additional, Heil, C., additional, Jeffrey, K.L., additional, Jennings, M.P., additional, Jin, P., additional, Johnson, A.C., additional, Kahaleh, B., additional, Kelly, D.R., additional, Abi Khalil, C., additional, Koufaris, C., additional, Kriska, A., additional, Kristiansen, S., additional, Kumar, A., additional, Kundakovic, M., additional, Lee, R.S., additional, Levenson, A.S., additional, Li, G., additional, Ligon, C.O., additional, Lu, Q., additional, Luo, S., additional, Lupien, M., additional, Mahnke, A.H., additional, Malek, N.P., additional, Marroncelli, N., additional, Mehta, S., additional, Merbs, S.L., additional, Miller, R.L., additional, Miranda, R.C., additional, Moloney, R.D., additional, Moresi, V., additional, Moylan, C.A., additional, Murphy, S.K., additional, Nada, S., additional, Nagaraja, V., additional, Navada, S.C., additional, Nicolaidou, V., additional, Nucera, C., additional, Oliva, R., additional, Oliver, V.F., additional, Pagani, M., additional, Palacios, D., additional, Panzeri, I., additional, Patel, A., additional, Peng, H., additional, Pigna, E., additional, Prusator, D.K., additional, Raha, P., additional, Rossetti, G., additional, Salem, N.A., additional, Sananbenesi, F., additional, Schenk, A., additional, Seib, K.L., additional, Sharma, A., additional, Shu, L., additional, Singh, J., additional, Sölétormos, G., additional, Tajbakhsh, J., additional, Tollefsbol, T.O., additional, Torrellas, C., additional, Trojer, P., additional, Vaiserman, A., additional, van Bysterveldt, K.A., additional, Voyias, P.D., additional, Wang, H., additional, Wapenaar, H., additional, Xiao, J., additional, Zhang, Y., additional, Zhou, Z., additional, Zimmer, P., additional, and Zong, D., additional
- Published
- 2016
- Full Text
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16. Preparing for Resettlement Associated with Climate Change
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de Sherbinin, A., Castro, M., Gemenne, F., Cernea, M. M., Adamo, S., Fearnside, P. M., Krieger, G., Lahmani, S., Oliver-Smith, A., Pankhurst, A., Scudder, T., Singer, B., Tan, Y., Wannier, G., Boncour, P., Ehrhart, C., Hugo, G., Pandey, B., and Shi, G.
- Published
- 2011
17. Spliced isoforms of the cardiac Nav1.5 channel modify channel activation by distinct structural mechanisms
- Author
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Adamo S. Mancino, William G. Glass, Yuhao Yan, Philip C. Biggin, and Derek Bowie
- Subjects
Adult ,Physiology ,Mutation ,Infant, Newborn ,Humans ,Protein Isoforms ,Voltage-Gated Sodium Channels ,Molecular Dynamics Simulation ,Electrophysiological Phenomena ,NAV1.5 Voltage-Gated Sodium Channel - Abstract
Alternative splicing is an important cellular mechanism that fine tunes the gating properties of both voltage- and ligand-gated ion-channels. The cardiac voltage-gated sodium channel, Nav1.5, is subject to alternative splicing of the DI S3–S4 linker, which generates two types of channels with different activation properties. Here, we show that the gating differences between the adult (mH1) and neonatal (Nav1.5e) isoforms of Nav1.5 are mediated by two amino acid residues: Thr/Ser at position 207 and Asp/Lys at position 211. Electrophysiological experiments, in conjunction with molecular dynamics simulations, revealed that each residue contributes equally to the overall gating shifts in activation, but that the underlying structural mechanisms are different. Asp/Lys at position 211 acts through electrostatic interactions, whereas Thr/Ser at position 207 is predicted to alter the hydrogen bond network at the top of the S3 helix. These distinct structural mechanisms work together to modify movement of the voltage-sensitive S4 helix to bring about channel activation. Interestingly, mutation of the homologous Asp and Thr residues of the skeletal muscle isoform, Nav1.4, to Lys and Ser, respectively, confers a similar gating shift in channel activation, suggesting that these residues may fulfill a conserved role across other Nav channel family members.
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- 2022
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18. Le forme semplificate del bilancio
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Fasiello, R, Adamo, S., Nicola Di Cagno - Stefano Adamo, Fasiello, R, and Adamo, S.
- Subjects
bilancio in forma abbreviata, valutazione, nota integrativa, semplificazioni micro-imprese - Published
- 2021
19. Spliced isoforms of the cardiac Nav1.5 channel modify channel activation by distinct structural mechanisms
- Author
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Mancino, Adamo S., primary, Glass, William G., additional, Yan, Yuhao, additional, Biggin, Philip C., additional, and Bowie, Derek, additional
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- 2022
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- View/download PDF
20. Failure Analysis of AlGaN/GaN Power HEMTs through an innovative sample preparation approach
- Author
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Torrisi, R. L., primary, Adamo, S., additional, Alessandrino, S., additional, Bottari, C., additional, Carbone, B., additional, Palmisciano, M., additional, and Vitanza, E., additional
- Published
- 2022
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21. Acetylcholine May Regulate its Own Nicotinic Receptor-Channel through the C-Kinase System
- Author
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Eusebi, F., Grassi, F., Nervi, C., Caporale, C., Adamo, S., Zani, B. M., and Molinaro, M.
- Published
- 1987
22. The Response of an Insect Parasitoid, Ormia ochracea (Tachinidae), to the Uncertainty of Larval Success during Infestation
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Adamo, S. A., Robert, D., Perez, J., and Hoy, R. R.
- Published
- 1995
23. Context dependency and generality of fever in insects
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Stahlschmidt, Z. R. and Adamo, S. A.
- Published
- 2013
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24. Closed-state inactivation of cardiac, skeletal, and neuronal sodium channels is isoform specific
- Author
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Niklas Brake, Adamo S. Mancino, Yuhao Yan, Takushi Shimomura, Yoshihiro Kubo, Anmar Khadra, and Derek Bowie
- Subjects
Physiology ,Action Potentials ,Protein Isoforms ,Ion Channel Gating ,Sodium Channels ,Membrane Potentials - Abstract
Voltage-gated sodium (Nav) channels produce the upstroke of action potentials in excitable tissues throughout the body. The gating of these channels is determined by the asynchronous movements of four voltage-sensing domains (VSDs). Past studies on the skeletal muscle Nav1.4 channel have indicated that VSD-I, -II, and -III are sufficient for pore opening, whereas VSD-IV movement is sufficient for channel inactivation. Here, we studied the cardiac sodium channel, Nav1.5, using charge-neutralizing mutations and voltage-clamp fluorometry. Our results reveal that both VSD-III and -IV are necessary for Nav1.5 inactivation, and that steady-state inactivation can be modulated by all VSDs. We also demonstrate that channel activation is partially determined by VSD-IV movement. Kinetic modeling suggests that these observations can be explained from the cardiac channel’s propensity to enter closed-state inactivation (CSI), which is significantly higher than that of other Nav channels. We show that skeletal muscle Nav1.4, cardiac Nav1.5, and neuronal Nav1.6 all have different propensities for CSI and postulate that these differences produce isoform-dependent roles for the four VSDs.
- Published
- 2021
25. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
- Author
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Klionsky, DJ, Abdel-Aziz, AK, Abdelfatah, S, Abdellatif, M, Abdoli, A, Abel, S, Abeliovich, H, Abildgaard, MH, Abudu, YP, Acevedo-Arozena, A, Adamopoulos, IE, Adeli, K, Adolph, TE, Adornetto, A, Aflaki, E, Agam, G, Agarwal, A, Aggarwal, BB, Agnello, M, Agostinis, P, Agrewala, JN, Agrotis, A, Aguilar, PV, Ahmad, ST, Ahmed, ZM, Ahumada-Castro, U, Aits, S, Aizawa, S, Akkoc, Y, Akoumianaki, T, Akpinar, HA, Al-Abd, AM, Al-Akra, L, Al-Gharaibeh, A, Alaoui-Jamali, MA, Alberti, S, Alcocer-Gómez, E, Alessandri, C, Ali, M, Alim Al-Bari, MA, Aliwaini, S, Alizadeh, J, Almacellas, E, Almasan, A, Alonso, A, Alonso, GD, Altan-Bonnet, N, Altieri, DC, Álvarez, ÉMC, Alves, S, Alves da Costa, C, Alzaharna, MM, Amadio, M, Amantini, C, Amaral, C, Ambrosio, S, Amer, AO, Ammanathan, V, An, Z, Andersen, SU, Andrabi, SA, Andrade-Silva, M, Andres, AM, Angelini, S, Ann, D, Anozie, UC, Ansari, MY, Antas, P, Antebi, A, Antón, Z, Anwar, T, Apetoh, L, Apostolova, N, Araki, T, Araki, Y, Arasaki, K, Araújo, WL, Araya, J, Arden, C, Arévalo, M-A, Arguelles, S, Arias, E, Arikkath, J, Arimoto, H, Ariosa, AR, Armstrong-James, D, Arnauné-Pelloquin, L, Aroca, A, Arroyo, DS, Arsov, I, Artero, R, Asaro, DML, Aschner, M, Ashrafizadeh, M, Ashur-Fabian, O, Atanasov, AG, Au, AK, Auberger, P, Auner, HW, Aurelian, L, Autelli, R, Avagliano, L, Ávalos, Y, Aveic, S, Aveleira, CA, Avin-Wittenberg, T, Aydin, Y, Ayton, S, Ayyadevara, S, Azzopardi, M, Baba, M, Backer, JM, Backues, SK, Bae, D-H, Bae, O-N, Bae, SH, Baehrecke, EH, Baek, A, Baek, S-H, Baek, SH, Bagetta, G, Bagniewska-Zadworna, A, Bai, H, Bai, J, Bai, X, Bai, Y, Bairagi, N, Baksi, S, Balbi, T, Baldari, CT, Balduini, W, Ballabio, A, Ballester, M, Balazadeh, S, Balzan, R, Bandopadhyay, R, Banerjee, S, Bánréti, Á, Bao, Y, Baptista, MS, Baracca, A, Barbati, C, Bargiela, A, Barilà, D, Barlow, PG, Barmada, SJ, Barreiro, E, Barreto, GE, Bartek, J, Bartel, B, Bartolome, A, Barve, GR, Basagoudanavar, SH, Bassham, DC, Bast, RC, Basu, A, Batoko, H, Batten, I, Baulieu, EE, Baumgarner, BL, Bayry, J, Beale, R, Beau, I, Beaumatin, F, Bechara, LRG, Beck, GR, Beers, MF, Begun, J, Behrends, C, Behrens, GMN, Bei, R, Bejarano, E, Bel, S, Behl, C, Belaid, A, Belgareh-Touzé, N, Bellarosa, C, Belleudi, F, Belló Pérez, M, Bello-Morales, R, Beltran, JSDO, Beltran, S, Benbrook, DM, Bendorius, M, Benitez, BA, Benito-Cuesta, I, Bensalem, J, Berchtold, MW, Berezowska, S, Bergamaschi, D, Bergami, M, Bergmann, A, Berliocchi, L, Berlioz-Torrent, C, Bernard, A, Berthoux, L, Besirli, CG, Besteiro, S, Betin, VM, Beyaert, R, Bezbradica, JS, Bhaskar, K, Bhatia-Kissova, I, Bhattacharya, R, Bhattacharya, S, Bhattacharyya, S, Bhuiyan, MS, Bhutia, SK, Bi, L, Bi, X, Biden, TJ, Bijian, K, Billes, VA, Binart, N, Bincoletto, C, Birgisdottir, AB, Bjorkoy, G, Blanco, G, Blas-Garcia, A, Blasiak, J, Blomgran, R, Blomgren, K, Blum, JS, Boada-Romero, E, Boban, M, Boesze-Battaglia, K, Boeuf, P, Boland, B, Bomont, P, Bonaldo, P, Bonam, SR, Bonfili, L, Bonifacino, JS, Boone, BA, Bootman, MD, Bordi, M, Borner, C, Bornhauser, BC, Borthakur, G, Bosch, J, Bose, S, Botana, LM, Botas, J, Boulanger, CM, Boulton, ME, Bourdenx, M, Bourgeois, B, Bourke, NM, Bousquet, G, Boya, P, Bozhkov, PV, Bozi, LHM, Bozkurt, TO, Brackney, DE, Brandts, CH, Braun, RJ, Braus, GH, Bravo-Sagua, R, Bravo-San Pedro, JM, Brest, P, Bringer, M-A, Briones-Herrera, A, Broaddus, VC, Brodersen, P, Brodsky, JL, Brody, SL, Bronson, PG, Bronstein, JM, Brown, CN, Brown, RE, Brum, PC, Brumell, JH, Brunetti-Pierri, N, Bruno, D, Bryson-Richardson, RJ, Bucci, C, Buchrieser, C, Bueno, M, Buitrago-Molina, LE, Buraschi, S, Buch, S, Buchan, JR, Buckingham, EM, Budak, H, Budini, M, Bultynck, G, Burada, F, Burgoyne, JR, Burón, MI, Bustos, V, Büttner, S, Butturini, E, Byrd, A, Cabas, I, Cabrera-Benitez, S, Cadwell, K, Cai, J, Cai, L, Cai, Q, Cairó, M, Calbet, JA, Caldwell, GA, Caldwell, KA, Call, JA, Calvani, R, Calvo, AC, Calvo-Rubio Barrera, M, Camara, NO, Camonis, JH, Camougrand, N, Campanella, M, Campbell, EM, Campbell-Valois, F-X, Campello, S, Campesi, I, Campos, JC, Camuzard, O, Cancino, J, Candido de Almeida, D, Canesi, L, Caniggia, I, Canonico, B, Cantí, C, Cao, B, Caraglia, M, Caramés, B, Carchman, EH, Cardenal-Muñoz, E, Cardenas, C, Cardenas, L, Cardoso, SM, Carew, JS, Carle, GF, Carleton, G, Carloni, S, Carmona-Gutierrez, D, Carneiro, LA, Carnevali, O, Carosi, JM, Carra, S, Carrier, A, Carrier, L, Carroll, B, Carter, AB, Carvalho, AN, Casanova, M, Casas, C, Casas, J, Cassioli, C, Castillo, EF, Castillo, K, Castillo-Lluva, S, Castoldi, F, Castori, M, Castro, AF, Castro-Caldas, M, Castro-Hernandez, J, Castro-Obregon, S, Catz, SD, Cavadas, C, Cavaliere, F, Cavallini, G, Cavinato, M, Cayuela, ML, Cebollada Rica, P, Cecarini, V, Cecconi, F, Cechowska-Pasko, M, Cenci, S, Ceperuelo-Mallafré, V, Cerqueira, JJ, Cerutti, JM, Cervia, D, Cetintas, VB, Cetrullo, S, Chae, H-J, Chagin, AS, Chai, C-Y, Chakrabarti, G, Chakrabarti, O, Chakraborty, T, Chami, M, Chamilos, G, Chan, DW, Chan, EYW, Chan, ED, Chan, HYE, Chan, HH, Chan, H, Chan, MTV, Chan, YS, Chandra, PK, Chang, C-P, Chang, C, Chang, H-C, Chang, K, Chao, J, Chapman, T, Charlet-Berguerand, N, Chatterjee, S, Chaube, SK, Chaudhary, A, Chauhan, S, Chaum, E, Checler, F, Cheetham, ME, Chen, C-S, Chen, G-C, Chen, J-F, Chen, LL, Chen, L, Chen, M, Chen, M-K, Chen, N, Chen, Q, Chen, R-H, Chen, S, Chen, W, Chen, X-M, Chen, X-W, Chen, X, Chen, Y, Chen, Y-G, Chen, Y-J, Chen, Y-Q, Chen, ZS, Chen, Z, Chen, Z-H, Chen, ZJ, Cheng, H, Cheng, J, Cheng, S-Y, Cheng, W, Cheng, X, Cheng, X-T, Cheng, Y, Cheng, Z, Cheong, H, Cheong, JK, Chernyak, BV, Cherry, S, Cheung, CFR, Cheung, CHA, Cheung, K-H, Chevet, E, Chi, RJ, Chiang, AKS, Chiaradonna, F, Chiarelli, R, Chiariello, M, Chica, N, Chiocca, S, Chiong, M, Chiou, S-H, Chiramel, AI, Chiurchiù, V, Cho, D-H, Choe, S-K, Choi, AMK, Choi, ME, Choudhury, KR, Chow, NS, Chu, CT, Chua, JP, Chua, JJE, Chung, H, Chung, KP, Chung, S, Chung, S-H, Chung, Y-L, Cianfanelli, V, Ciechomska, IA, Cifuentes, M, 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- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
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- 2021
26. Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505/515
- Author
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Südfeld, C.A.K.B., Hubáček, M., Adamo, S., D', Wijffels, R.H., Barbosa, M.J., Südfeld, C.A.K.B., Hubáček, M., Adamo, S., D', Wijffels, R.H., and Barbosa, M.J.
- Abstract
Background Microalgae are considered a promising platform for sustainable lipid production. Despite this, productivities need to be improved to facilitate an economically viable production process. This can be achieved through strain improvement, for instance by genetic engineering. Strain improvement strategies often deploy high-throughput screening platforms i.a. involving single-cell methodologies such as fluorescence-activated cell sorting (FACS) for the identification and isolation of better-performing strains. The heterokont microalga Nannochloropsis is a prospective candidate for the industrial production of lipids. Previous studies have reported the isolation of high lipid-producing Nannochloropsis strains by combining qualitative staining of lipid bodies using the fluorophoric dye BODIPY with FACS methodology. However, it has never been investigated how cellular physiology and different staining conditions hamper the reproducibility of this method as a quantitative screening procedure. Results Here we report the development of an optimized single cell lipid screening procedure for Nannochloropsis oceanica. Systematic assessment of different staining conditions revealed that treatment with 6% DMSO and 1.2 μg ml−1 BODIPY for 15 min is ideal for staining neutral lipids in an exponentially growing culture of N. oceanica. Cultures that are overproducing lipids, for example after exposure to external stimuli such as nutrient deprivation stress, require treatment with 10% DMSO and 1.2–1.6 μg ml−1 BODIPY for 36 min to facilitate complete staining of lipid bodies. We verify that DMSO is required to permeabilize the particularly tough cell barrier of Nannochloropsis and we show that exposure to 10% DMSO does not affect cell viability. Increasing concentrations of BODIPY, however, correlated with a decrease in viability when screening stressed cultures. Using the optimized protocol, reproductive viabilities can be expected to be ~91% and 83–82% for no
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- 2021
27. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).
- Author
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Quadrilatero J., Walker D.W., Walker M.J., Walker S.A., Walter J., Wandosell F.G., Wang B., Wang C., Wang C.-Y., Wang D., Wang F., Wang G., Wang H., Wang H.-G., Wang J., Wang K., Wang L., Wang M.H., Wang M., Wang N., Wang P., Wang Q.J., Wang Q., Wang Q.K., Wang Q.A., Wang W.-T., Wang W., Wang X., Wang Y.-Y., Wang Y., Wang Z., Warnes G., Warnsmann V., Watada H., Watanabe E., Watchon M., Wawrzynska A., Weaver T.E., Wegrzyn G., Wehman A.M., Wei H., Wei L., Wei T., Wei Y., Weiergraber O.H., Weihl C.C., Weindl G., Weiskirchen R., Wells A., Wen R.H., Wen X., Werner A., Weykopf B., Wheatley S.P., W... [Ovid has truncated this field to avoid Excel character limitations], Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., Anton Z., Anwar T., Apetoh L., Apostolova N., Araki T., Araki Y., Arasaki K., Araujo W.L., Araya J., Arden C., Arevalo M.-A., Arguelles S., Arias E., Arikkath J., Arimoto H., Ariosa A.R., Armstrong-James D., Arnaune-Pelloquin L., Aroca A., Arroyo D.S., Arsov I., Artero R., Asaro D.M.L., Aschner M., Ashrafizadeh M., Ashur-Fabian O., Atanasov A.G., Au A.K., Auberger P., Auner H.W., Aurelian L., Autelli R., Avagliano L., Avalos Y., Aveic S., Aveleira C.A., Avin-Wittenberg T., Aydin Y., Ayton S., Ayyadevara S., Azzopardi M., Baba M., Backer J.M., Backues S.K., Bae D.-H., Bae O.-N., Bae S.H., Baehrecke E.H., Baek A., Baek S.-H., Baek S.H., Bagetta G., Bagniewska-Zadworna A., Bai H., Bai J., Bai X., Bai Y., Bairagi N., Baksi S., Balbi T., Baldari C.T., Balduini W., Ballabio A., Ballester M., Balazadeh S., Balzan R., Bandopadhyay R., Banerjee S., Banreti A., Bao Y., Baptista M.S., Baracca A., Barbati C., Bargiela A., Barila D., Barlow P.G., Barmada S.J., Barreiro E., Barreto G.E., Bartek J., Bartel B., Bartolome A., Barve G.R., Basagoudanavar S.H., Bassham D.C., Bast R.C., Basu A., Batoko H., Batten I., Baulieu E.E., Baumgarner B.L., Bayry J., Beale R., Beau I., Beaumatin F., Bechara L.R.G., Beck G.R., Beers M.F., Begun J., Behrends C., Behrens G.M.N., Bei R., Bejarano E., Bel S., Behl C., Belaid A., Belgareh-Touze N., Bellarosa C., Belleudi F., Bello Perez M., Bello-Morales R., Beltran J.S.D.O., Beltran S., Benbrook D.M., Bendorius M., Benitez B.A., Benito-Cuesta I., Bensalem J., Berchtold M.W., Berezowska S., Bergamaschi D., Bergami M., Bergmann A., Berliocchi L., Berlioz-Torrent C., Bernard A., Berthoux L., Besirli C.G., Besteiro S., Betin V.M., Beyaert R., Bezbradica J.S., Bhaskar K., Bhatia-Kissova I., Bhattacharya R., Bhattacharya S., Bhattacharyya S., Bhuiyan M.S., Bhutia S.K., Bi L., Bi X., Biden T.J., Bijian K., Billes V.A., Binart N., Bincoletto C., Birgisdottir A.B., Bjorkoy G., Blanco G., Blas-Garcia A., Blasiak J., Blomgran R., Blomgren K., Blum J.S., Boada-Romero E., Boban M., Boesze-Battaglia K., Boeuf P., Boland B., Bomont P., Bonaldo P., Bonam S.R., Bonfili L., Bonifacino J.S., Boone B.A., Bootman M.D., Bordi M., Borner C., Bornhauser B.C., Borthakur G., Bosch J., Bose S., Botana L.M., Botas J., Boulanger C.M., Boulton M.E., Bourdenx M., Bourgeois B., Bourke N.M., Bousquet G., Boya P., Bozhkov P.V., Bozi L.H.M., Bozkurt T.O., Brackney D.E., Brandts C.H., Braun R.J., Braus G.H., Bravo-Sagua R., Bravo-San Pedro J.M., Brest P., Bringer M.-A., Briones-Herrera A., Broaddus V.C., Brodersen P., Brodsky J.L., Brody S.L., Bronson P.G., Bronstein J.M., Brown C.N., Brown R.E., Brum P.C., Brumell J.H., Brunetti-Pierri N., Bruno D., Bryson-Richardson R.J., Bucci C., Buchrieser C., Bueno M., Buitrago-Molina L.E., Buraschi S., Buch S., Buchan J.R., Buckingham E.M., Budak H., Budini M., Bultynck G., Burada F., Burgoyne J.R., Buron M.I., Bustos V., Buttner S., Butturini E., Byrd A., Cabas I., Cabrera-Benitez S., Cadwell K., Cai J., Cai L., Cai Q., Cairo M., Calbet J.A., Caldwell G.A., Caldwell K.A., Call J.A., Calvani R., Calvo A.C., Calvo-Rubio Barrera M., Camara N.O.S., Camonis J.H., Camougrand N., Campanella M., Campbell E.M., Campbell-Valois F.-X., Campello S., Campesi I., Campos J.C., Camuzard O., Cancino J., Candido de Almeida D., Canesi L., Caniggia I., Canonico B., Canti C., Cao B., Caraglia M., Carames B., Carchman E.H., Cardenal-Munoz E., Cardenas C., Cardenas L., Cardoso S.M., Carew J.S., Carle G.F., Carleton G., Carloni S., Carmona-Gutierrez D., Carneiro L.A., Carnevali O., Carosi J.M., Carra S., Carrier A., Carrier L., Carroll B., Carter A.B., Carvalho A.N., Casanova M., Casas C., Casas J., Cassioli C., Castillo E.F., Castillo K., Castillo-Lluva S., Castoldi F., Castori M., Castro A.F., Castro-Caldas M., Castro-Hernandez J., Castro-Obregon S., Catz S.D., Cavadas C., Cavaliere F., Cavallini G., Cavinato M., Cayuela M.L., Cebollada Rica P., Cecarini V., Cecconi F., Cechowska-Pasko M., Cenci S., Ceperuelo-Mallafre V., Cerqueira J.J., Cerutti J.M., Cervia D., Cetintas V.B., Cetrullo S., Chae H.-J., Chagin A.S., Chai C.-Y., Chakrabarti G., Chakrabarti O., Chakraborty T., Chami M., Chamilos G., Chan D.W., Chan E.Y.W., Chan E.D., Chan H.Y.E., Chan H.H., Chan H., Chan M.T.V., Chan Y.S., Chandra P.K., Chang C.-P., Chang C., Chang H.-C., Chang K., Chao J., Chapman T., Charlet-Berguerand N., Chatterjee S., Chaube S.K., Chaudhary A., Chauhan S., Chaum E., Checler F., Cheetham M.E., Chen C.-S., Chen G.-C., Chen J.-F., Chen L.L., Chen L., Chen M., Chen M.-K., Chen N., Chen Q., Chen R.-H., Chen S., Chen W., Chen X.-M., Chen X.-W., Chen X., Chen Y.-G., Chen Y., Chen Y.-J., Chen Y.-Q., Chen Z.S., Chen Z.-H., Chen Z.J., Cheng H., Cheng J., Cheng S.-Y., Cheng W., Cheng X., Cheng X.-T., Cheng Y., Cheng Z., Chen Z., Cheong H., Cheong J.K., Chernyak B.V., Cherry S., Cheung C.F.R., Cheung C.H.A., Cheung K.-H., Chevet E., Chi R.J., Chiang A.K.S., Chiaradonna F., Chiarelli R., Chiariello M., Chica N., Chiocca S., Chiong M., Chiou S.-H., Chiramel A.I., Chiurchiu V., Cho D.-H., Choe S.-K., Choi A.M.K., Choi M.E., Choudhury K.R., Chow N.S., Chu C.T., Chua J.P., Chua J.J.E., Chung H., Chung K.P., Chung S., Chung S.-H., Chung Y.-L., Cianfanelli V., Ciechomska I.A., Cifuentes M., Cinque L., Cirak S., Cirone M., Clague M.J., Clarke R., Clementi E., Coccia E.M., Codogno P., Cohen E., Cohen M.M., Colasanti T., Colasuonno F., Colbert R.A., Colell A., Colic M., Coll N.S., Collins M.O., Colombo M.I., Colon-Ramos D.A., Combaret L., Comincini S., Cominetti M.R., Consiglio A., Conte A., Conti F., Contu V.R., Cookson M.R., Coombs K.M., Coppens I., Corasaniti M.T., Corkery D.P., Cordes N., Cortese K., Costa M.D.C., Costantino S., Costelli P., Coto-Montes A., Crack P.J., Crespo J.L., Criollo A., Crippa V., Cristofani R., Csizmadia T., Cuadrado A., Cui B., Cui J., Cui Y., Culetto E., Cumino A.C., Cybulsky A.V., Czaja M.J., Czuczwar S.J., D'Adamo S., D'Amelio M., D'Arcangelo D., D'Lugos A.C., D'Orazi G., da Silva J.A., Dafsari H.S., Dagda R.K., Dagdas Y., Daglia M., Dai X., Dai Y., Dal Col J., Dalhaimer P., Dalla Valle L., Dallenga T., Dalmasso G., Damme M., Dando I., Dantuma N.P., Darling A.L., Das H., Dasarathy S., Dasari S.K., Dash S., Daumke O., Dauphinee A.N., Davies J.S., Davila V.A., Davis R.J., Davis T., Dayalan Naidu S., De Amicis F., De Bosscher K., De Felice F., De Franceschi L., De Leonibus C., de Mattos Barbosa M.G., De Meyer G.R.Y., De Milito A., De Nunzio C., De Palma C., De Santi M., De Virgilio C., De Zio D., Debnath J., DeBosch B.J., Decuypere J.-P., Deehan M.A., Deflorian G., DeGregori J., Dehay B., Del Rio G., Delaney J.R., Delbridge L.M.D., Delorme-Axford E., Delpino M.V., Demarchi F., Dembitz V., Demers N.D., Deng H., Deng Z., Dengjel J., Dent P., Denton D., DePamphilis M.L., Der C.J., Deretic V., Descoteaux A., Devis L., Devkota S., Devuyst O., Dewson G., Dharmasivam M., Dhiman R., di Bernardo D., Di Cristina M., Di Domenico F., Di Fazio P., Di Fonzo A., Di Guardo G., Di Guglielmo G.M., Di Leo L., Di Malta C., Di Nardo A., Di Rienzo M., Di Sano F., Diallinas G., Diao J., Diaz-Araya G., Diaz-Laviada I., Dickinson J.M., Diederich M., Dieude M., Dikic I., Ding S., Ding W.-X., Dini L., Dinic J., Dinic M., Dinkova-Kostova A.T., Dionne M.S., Distler J.H.W., Diwan A., Dixon I.M.C., Djavaheri-Mergny M., Dobrinski I., Dobrovinskaya O., Dobrowolski R., Dobson R.C.J., Dokic J., Dokmeci Emre S., Donadelli M., Dong B., Dong X., Dong Z., Dorn II G.W., Dotsch V., Dou H., Dou J., Dowaidar M., Dridi S., Drucker L., Du A., Du C., Du G., Du H.-N., Du L.-L., du Toit A., Duan S.-B., Duan X., Duarte S.P., Dubrovska A., Dunlop E.A., Dupont N., Duran R.V., Dwarakanath B.S., Dyshlovoy S.A., Ebrahimi-Fakhari D., Eckhart L., Edelstein C.L., Efferth T., Eftekharpour E., Eichinger L., Eid N., Eisenberg T., Eissa N.T., Eissa S., Ejarque M., El Andaloussi A., El-Hage N., El-Naggar S., Eleuteri A.M., El-Shafey E.S., Elgendy M., Eliopoulos A.G., Elizalde M.M., Elks P.M., Elsasser H.-P., Elsherbiny E.S., Emerling B.M., Emre N.C.T., Eng C.H., Engedal N., Engelbrecht A.-M., Engelsen A.S.T., Enserink J.M., Escalante R., Esclatine A., Escobar-Henriques M., Eskelinen E.-L., Espert L., Eusebio M.-O., Fabrias G., Fabrizi C., Facchiano A., Facchiano F., Fadeel B., Fader C., Faesen A.C., Fairlie W.D., Falco A., Falkenburger B.H., Fan D., Fan J., Fan Y., Fang E.F., Fang Y., Fanto M., Farfel-Becker T., Faure M., Fazeli G., Fedele A.O., Feldman A.M., Feng D., Feng J., Feng L., Feng Y., Feng W., Fenz Araujo T., Ferguson T.A., Fernandez A.F., Fernandez-Checa J.C., Fernandez-Veledo S., Fernie A.R., Ferrante A.W., Ferraresi A., Ferrari M.F., Ferreira J.C.B., Ferro-Novick S., Figueras A., Filadi R., Filigheddu N., Filippi-Chiela E., Filomeni G., Fimia G.M., Fineschi V., Finetti F., Finkbeiner S., Fisher E.A., Fisher P.B., Flamigni F., Fliesler S.J., Flo T.H., Florance I., Florey O., Florio T., Fodor E., Follo C., Fon E.A., Forlino A., Fornai F., Fortini P., Fracassi A., Fraldi A., Franco B., Franco R., Franconi F., Frankel L.B., Friedman S.L., Frohlich L.F., Fruhbeck G., Fuentes J.M., Fujiki Y., Fujita N., Fujiwara Y., Fukuda M., Fulda S., Furic L., Furuya N., Fusco C., Gack M.U., Gaffke L., Galadari S., Galasso A., Galindo M.F., Gallolu Kankanamalage S., Galluzzi L., Galy V., Gammoh N., Gan B., Ganley I.G., Gao F., Gao H., Gao M., Gao P., Gao S.-J., Gao W., Gao X., Garcera A., Garcia M.N., Garcia V.E., Garcia-Del Portillo F., Garcia-Escudero V., Garcia-Garcia A., Garcia-Macia M., Garcia-Moreno D., Garcia-Ruiz C., Garcia-Sanz P., Garg A.D., Gargini R., Garofalo T., Garry R.F., Gassen N.C., Gatica D., Ge L., Ge W., Geiss-Friedlander R., Gelfi C., Genschik P., Gentle I.E., Gerbino V., Gerhardt C., Germain K., Germain M., Gewirtz D.A., Ghasemipour Afshar E., Ghavami S., Ghigo A., Ghosh M., Giamas G., Giampietri C., Giatromanolaki A., Gibson G.E., Gibson S.B., Ginet V., Giniger E., Giorgi C., Girao H., Girardin S.E., Giridharan M., Giuliano S., Giulivi C., Giuriato S., Giustiniani J., Gluschko A., Goder V., Goginashvili A., Golab J., Goldstone D.C., Golebiewska A., Gomes L.R., Gomez R., Gomez-Sanchez R., Gomez-Puerto M.C., Gomez-Sintes R., Gong Q., Goni F.M., Gonzalez-Gallego J., Gonzalez-Hernandez T., Gonzalez-Polo R.A., Gonzalez-Reyes J.A., Gonzalez-Rodriguez P., Goping I.S., Gorbatyuk M.S., Gorbunov N.V., Gorgulu K., Gorojod R.M., Gorski S.M., Goruppi S., Gotor C., Gottlieb R.A., Gozes I., Gozuacik D., Graef M., Graler M.H., Granatiero V., Grasso D., Gray J.P., Green D.R., Greenhough A., Gregory S.L., Griffin E.F., Grinstaff M.W., Gros F., Grose C., Gross A.S., Gruber F., Grumati P., Grune T., Gu X., Guan J.-L., Guardia C.M., Guda K., Guerra F., Guerri C., Guha P., Guillen C., Gujar S., Gukovskaya A., Gukovsky I., Gunst J., Gunther A., Guntur A.R., Guo C., Guo H., Guo L.-W., Guo M., Gupta P., Gupta S.K., Gupta S., Gupta V.B., Gupta V., Gustafsson A.B., Gutterman D.D., H.B R., Haapasalo A., Haber J.E., Hac A., Hadano S., Hafren A.J., Haidar M., Hall B.S., Hallden G., Hamacher-Brady A., Hamann A., Hamasaki M., Han W., Hansen M., Hanson P.I., Hao Z., Harada M., Harhaji-Trajkovic L., Hariharan N., Haroon N., Harris J., Hasegawa T., Hasima Nagoor N., Haspel J.A., Haucke V., Hawkins W.D., Hay B.A., Haynes C.M., Hayrabedyan S.B., Hays T.S., He C., He Q., He R.-R., He Y.-W., He Y.-Y., Heakal Y., Heberle A.M., Hejtmancik J.F., Helgason G.V., Henkel V., Herb M., Hergovich A., Herman-Antosiewicz A., Hernandez A., Hernandez C., Hernandez-Diaz S., Hernandez-Gea V., Herpin A., Herreros J., Hervas J.H., Hesselson D., Hetz C., Heussler V.T., Higuchi Y., Hilfiker S., Hill J.A., Hlavacek W.S., Ho E.A., Ho I.H.T., Ho P.W.-L., Ho S.-L., Ho W.Y., Hobbs G.A., Hochstrasser M., Hoet P.H.M., Hofius D., Hofman P., Hohn A., Holmberg C.I., Hombrebueno J.R., Yi-Ren Hong C.-W.H., Hooper L.V., Hoppe T., Horos R., Hoshida Y., Hsin I.-L., Hsu H.-Y., Hu B., Hu D., Hu L.-F., Hu M.C., Hu R., Hu W., Hu Y.-C., Hu Z.-W., Hua F., Hua J., Hua Y., Huan C., Huang C., Huang H., Huang K., Huang M.L.H., Huang R., Huang S., Huang T., Huang X., Huang Y.J., Huber T.B., Hubert V., Hubner C.A., Hughes S.M., Hughes W.E., Humbert M., Hummer G., Hurley J.H., Hussain S., Hussey P.J., Hutabarat M., Hwang H.-Y., Hwang S., Ieni A., Ikeda F., Imagawa Y., Imai Y., Imbriano C., Imoto M., Inman D.M., Inoki K., Iovanna J., Iozzo R.V., Ippolito G., Irazoqui J.E., Iribarren P., Ishaq M., Ishikawa M., Ishimwe N., Isidoro C., Ismail N., Issazadeh-Navikas S., Itakura E., Ito D., Ivankovic D., Ivanova S., Iyer A.K.V., Izquierdo J.M., Izumi M., Jaattela M., Jabir M.S., Jackson W.T., Jacobo-Herrera N., Jacomin A.-C., Jacquin E., Jadiya P., Jaeschke H., Jagannath C., Jakobi A.J., Jakobsson J., Janji B., Jansen-Durr P., Jansson P.J., Jantsch J., Januszewski S., Jassey A., Jean S., Jeltsch-David H., Jendelova P., Jenny A., Jensen T.E., Jessen N., Jewell J.L., Ji J., Jia L., Jia R., Jiang L., Jiang Q., Jiang R., Jiang T., Jiang X., Jiang Y., Jimenez-Sanchez M., Jin E.-J., Jin F., Jin H., Jin L., Jin M., Jin S., Jo E.-K., Joffre C., Johansen T., Johnson G.V.W., Johnston S.A., Jokitalo E., Jolly M.K., Joosten L.A.B., Jordan J., Joseph B., Ju D., Ju J.-S., Ju J., Juarez E., Judith D., Juhasz G., Jun Y., Jung C.H., Jung S.-C., Jung Y.K., Jungbluth H., Jungverdorben J., Just S., Kaarniranta K., Kaasik A., Kabuta T., Kaganovich D., Kahana A., Kain R., Kajimura S., Kalamvoki M., Kalia M., Kalinowski D.S., Kaludercic N., Kalvari I., Kaminska J., Kaminskyy V.O., Kanamori H., Kanasaki K., Kang C., Kang R., Kang S.S., Kaniyappan S., Kanki T., Kanneganti T.-D., Kanthasamy A.G., Kanthasamy A., Kantorow M., Kapuy O., Karamouzis M.V., Karim M.R., Karmakar P., Katare R.G., Kato M., Kaufmann S.H.E., Kauppinen A., Kaushal G.P., Kaushik S., Kawasaki K., Kazan K., Ke P.-Y., Keating D.J., Keber U., Kehrl J.H., Keller K.E., Keller C.W., Kemper J.K., Kenific C.M., Kepp O., Kermorgant S., Kern A., Ketteler R., Keulers T.G., Khalfin B., Khalil H., Khambu B., Khan S.Y., Khandelwal V.K.M., Khandia R., Kho W., Khobrekar N.V., Khuansuwan S., Khundadze M., Killackey S.A., Kim D., Kim D.R., Kim D.-H., Kim D.-E., Kim E.Y., Kim E.-K., Kim H.-R., Kim H.-S., Hyung-Ryong Kim, Kim J.K., Kim J.-H., Kim J., Kim J.H., Kim K.I., Kim P.K., Kim S.-J., Kimball S.R., Kimchi A., Kimmelman A.C., Kimura T., King M.A., Kinghorn K.J., Kinsey C.G., Kirkin V., Kirshenbaum L.A., Kiselev S.L., Kishi S., Kitamoto K., Kitaoka Y., Kitazato K., Kitsis R.N., Kittler J.T., Kjaerulff O., Klein P.S., Klopstock T., Klucken J., Knaevelsrud H., Knorr R.L., Ko B.C.B., Ko F., Ko J.-L., Kobayashi H., Kobayashi S., Koch I., Koch J.C., Koenig U., Kogel D., Koh Y.H., Koike M., Kohlwein S.D., Kocaturk N.M., Komatsu M., Konig J., Kono T., Kopp B.T., Korcsmaros T., Korkmaz G., Korolchuk V.I., Korsnes M.S., Koskela A., Kota J., Kotake Y., Kotler M.L., Kou Y., Koukourakis M.I., Koustas E., Kovacs A.L., Kovacs T., Koya D., Kozako T., Kraft C., Krainc D., Kramer H., Krasnodembskaya A.D., Kretz-Remy C., Kroemer G., Ktistakis N.T., Kuchitsu K., Kuenen S., Kuerschner L., Kukar T., Kumar A., Kumar D., Kumar S., Kume S., Kumsta C., Kundu C.N., Kundu M., Kunnumakkara A.B., Kurgan L., Kutateladze T.G., Kutlu O., Kwak S., Kwon H.J., Kwon T.K., Kwon Y.T., Kyrmizi I., La Spada A., Labonte P., Ladoire S., Laface I., Lafont F., Lagace D.C., Lahiri V., Lai Z., Laird A.S., Lakkaraju A., Lamark T., Lan S.-H., Landajuela A., Lane D.J.R., Lane J.D., Lang C.H., Lange C., Langel U., Langer R., Lapaquette P., Laporte J., LaRusso N.F., Lastres-Becker I., Lau W.C.Y., Laurie G.W., Lavandero S., Law B.Y.K., Law H.K.-W., Layfield R., Le W., Le Stunff H., Leary A.Y., Lebrun J.-J., Leck L.Y.W., Leduc-Gaudet J.-P., Lee C., Lee C.-P., Lee D.-H., Lee E.B., Lee E.F., Lee G.M., Lee H.-J., Lee H.K., Lee J.M., Lee J.S., Lee J.-A., Lee J.-Y., Lee J.H., Lee M., Lee M.G., Lee M.J., Lee M.-S., Lee S.-J., Lee S.Y., Lee S.B., Lee W.H., Lee Y.-R., Lee Y.-H., Lee Y., Lefebvre C., Legouis R., Lei Y.L., Lei Y., Leikin S., Leitinger G., Lemus L., Leng S., Lenoir O., Lenz G., Lenz H.J., Lenzi P., Leon Y., Leopoldino A.M., Leschczyk C., Leskela S., Letellier E., Leung C.-T., Leung P.S., Leventhal J.S., Levine B., Lewis P.A., Ley K., Li B., Li D.-Q., Li J., Li K., Li L., Li M., Li P.-L., Li M.-Q., Li Q., Li S., Li T., Li W., Li X., Li Y.-P., Li Y., Lian J., Liang C., Liang Q., Liang W., Liang Y., Liao G., Liao L., Liao M., Liao Y.-F., Librizzi M., Lie P.P.Y., Lilly M.A., Lim H.J., Lima T.R.R., Limana F., Lin C., Lin C.-W., Lin D.-S., Lin F.-C., Lin J.D., Lin K.M., Lin K.-H., Lin L.-T., Lin P.-H., Lin Q., Lin S., Lin S.-J., Lin W., Lin X., Lin Y.-X., Lin Y.-S., Linden R., Lindner P., Ling S.-C., Lingor P., Linnemann A.K., Liou Y.-C., Lipinski M.M., Lipovsek S., Lira V.A., Lisiak N., Liton P.B., Liu C., Liu C.-H., Liu C.-F., Liu C.H., Liu F., Liu H.-S., Liu H.-F., Liu H., Liu J., Liu L., Liu M., Liu Q., Liu W., Liu X.-H., Liu X., Liu Y., Livingston J.A., Lizard G., Lizcano J.M., Ljubojevic-Holzer S., LLeonart 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X., Qi Y., Qian W., Qiang L., Qiu Y., Quarleri J., Raben N., Rabinowich H., Ragona D., Ragusa M.J., Rahimi N., Rahmati M., Raia V., Raimundo N., Rajasekaran N.-S., Ramachandra Rao S., Rami A., Ramirez-Pardo I., Ramsden D.B., Randow F., Rangarajan P.N., Ranieri D., Rao H., Rao L., Rao R., Rathore S., Ratnayaka J.A., Ratovitski E.A., Ravanan P., Ravegnini G., Ray S.K., Razani B., Rebecca V., Reggiori F., Regnier-Vigouroux A., Reichert A.S., Reigada D., Reiling J.H., Rein T., Reipert S., Rekha R.S., Ren H., Ren J., Ren W., Renault T., Renga G., Reue K., Rewitz K., Ribeiro de Andrade Ramos B., Riazuddin S.A., Ribeiro-Rodrigues T.M., Ricci J.-E., Ricci R., Riccio V., Richardson D.R., Rikihisa Y., Risbud M.V., Risueno R.M., Ritis K., Rizza S., Rizzuto R., Roberts H.C., Roberts L.D., Robinson K.J., Roccheri M.C., Rocchi S., Rodney G.G., Rodrigues T., Rodrigues Silva V.R., Rodriguez A., Rodriguez-Barrueco R., Rodriguez-Henche N., Rodriguez-Rocha H., Roelofs J., Rogers R.S., Rogov V.V., Rojo 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Viret C., Viscomi M.T., Visnjic D., Vitale I., Vocadlo D.J., Voitsekhovskaja O.V., Volonte C., Volta M., Vomero M., Von Haefen C., Vooijs M.A., Voos W., Vucicevic L., Wade-Martins R., Waguri S., Waite K.A., and Wakatsuki S.
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.Copyright © 2020 Informa UK Limited, trading as Taylor & Francis Group.
- Published
- 2021
28. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
- Author
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Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., Anton Z., Anwar T., Apetoh L., Apostolova N., Araki T., Araki Y., Arasaki K., Araujo W.L., Araya J., Arden C., Arevalo M.-A., Arguelles S., Arias E., Arikkath J., Arimoto H., Ariosa A.R., Armstrong-James D., Arnaune-Pelloquin L., Aroca A., Arroyo D.S., Arsov I., Artero R., Asaro D.M.L., Aschner M., Ashrafizadeh M., Ashur-Fabian O., Atanasov A.G., Au A.K., Auberger P., Auner H.W., Aurelian L., Autelli R., Avagliano L., Avalos Y., Aveic S., Aveleira C.A., Avin-Wittenberg T., Aydin Y., Ayton S., Ayyadevara S., Azzopardi M., Baba M., Backer J.M., Backues S.K., Bae D.-H., Bae O.-N., Bae S.H., Baehrecke E.H., Baek A., Baek S.-H., Baek S.H., Bagetta G., Bagniewska-Zadworna A., Bai H., Bai J., Bai X., Bai Y., Bairagi N., Baksi S., Balbi T., Baldari C.T., Balduini W., Ballabio A., Ballester M., Balazadeh S., Balzan R., Bandopadhyay R., Banerjee S., Banreti A., Bao Y., Baptista M.S., Baracca A., Barbati C., Bargiela A., Barila D., Barlow P.G., Barmada S.J., Barreiro E., Barreto G.E., Bartek J., Bartel B., Bartolome A., Barve G.R., Basagoudanavar S.H., Bassham 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[Ovid has truncated this field to avoid Excel character limitations], Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., 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- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.Copyright © 2020 Informa UK Limited, trading as Taylor & Francis Group.
- Published
- 2021
29. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
- Author
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Saftig, Paul, Sagona, Antonia P, Sahay, Gaurav, Sahebkar, Amirhossein, Sahin, Mustafa, Sahin, Ozgur, Sahni, Sumit, Saito, Nayuta, Saito, Shigeru, Saito, Tsunenori, Sakai, Ryohei, Sakai, Yasuyoshi, Sakamaki, Jun-Ichi, Saksela, Kalle, Salazar, Gloria, Salazar-Degracia, Anna, Salekdeh, Ghasem H, Saluja, Ashok K, Sampaio-Marques, Belém, Sanchez, Maria Cecilia, Sanchez-Alcazar, Jose A, Sanchez-Vera, Victoria, Sancho-Shimizu, Vanessa, Sanderson, J Thomas, Sandri, Marco, Santaguida, Stefano, Santambrogio, Laura, Santana, Magda M, Santoni, Giorgio, Sanz, Alberto, Sanz, Pascual, Saran, Shweta, Sardiello, Marco, Sargeant, Timothy J, Sarin, Apurva, Sarkar, Chinmoy, Sarkar, Sovan, Sarrias, Maria-Rosa, Sarkar, Surajit, Sarmah, Dipanka Tanu, Sarparanta, Jaakko, Sathyanarayan, Aishwarya, Sathyanarayanan, Ranganayaki, Scaglione, K Matthew, Scatozza, Francesca, Schaefer, Liliana, Schafer, Zachary T, Schaible, Ulrich E, Schapira, Anthony H V, Scharl, Michael, Schatzl, Hermann M, Schein, Catherine H, 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Yu, Seong-Woon, Yu, Victor C, Yu, W Haung, Yu, Zhengping, Yu, Zhou, Yuan, Junying, Yuan, Ling-Qing, Yuan, Shilin, Yuan, Shyng-Shiou F, Yuan, Yanggang, Yuan, Zengqiang, Yue, Jianbo, Yue, Zhenyu, Yun, Jeanho, Yung, Raymond L, Zacks, David N, Zaffagnini, Gabriele, Zambelli, Vanessa O, Zanella, Isabella, Zang, Qun S, Zanivan, Sara, Zappavigna, Silvia, Zaragoza, Pilar, Zarbalis, Konstantinos S, Zarebkohan, Amir, Zarrouk, Amira, Zeitlin, Scott O, Zeng, Jialiu, Zeng, Ju-Deng, Žerovnik, Eva, Zhan, Lixuan, Zhang, Bin, Zhang, Donna D, Zhang, Hanlin, Zhang, Hong, Zhang, Honghe, Zhang, Huafeng, Zhang, Huaye, Zhang, Hui, Zhang, Hui-Ling, Zhang, Jianbin, Zhang, Jianhua, Zhang, Jing-Pu, Zhang, Kalin Y B, Zhang, Leshuai W, Zhang, Lin, Zhang, Lisheng, Zhang, Lu, Zhang, Luoying, Zhang, Menghuan, Zhang, Peng, Zhang, Sheng, Zhang, Wei, Zhang, Xiangnan, Zhang, Xiao-Wei, Zhang, Xiaolei, Zhang, Xiaoyan, Zhang, Xin, Zhang, Xinxin, Zhang, Xu Dong, Zhang, Yang, Zhang, Yanjin, Zhang, Yi, Zhang, Ying-Dong, Zhang, 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Werner, Brand-Saberi, Beate, Dong, X Charlie, Kenchappa, Chandra Shekar, Li, Zuguo, Lin, Yong, Oshima, Shigeru, Rong, Yueguang, Sluimer, Judith C, Stallings, Christina L, and Tong, Chun-Kit
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
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- 2021
30. A “CRISPY” tale on microalgal genome editing: featuring Nannochloropsis oceanica IMET1
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van der Oost, J., Barbosa, M.J., D’ Adamo, S., Naduthodi, Mihris Ibnu Saleem, van der Oost, J., Barbosa, M.J., D’ Adamo, S., and Naduthodi, Mihris Ibnu Saleem
- Abstract
Microalgae are unicellular microbes that can grow under autotrophic conditions to produce valuable compounds such as omega-3 fatty acids, vitamins and lipids. Moreover, these organisms are reported to have faster growth rates and improved photosynthetic efficiencies compared to land plants. These characters make them a promising candidate for sustainable production of green chemicals.The lipids that are produced by microalgae can be used for producing biofuels. Oleaginous microalgae such as Nannochloropsis oceanica, is found to accumulate more than 50% of its dry weight with lipids under specific conditions. As microalgae cultivation does not compete with land areas that are used for food and feed crop production, this could be a better alternative to the biofuels that are produced from land plants.The high lipid accumulation in N. oceanica is reported to be triggered under nitrogen stress. However, under these conditions, the growth rate is reduced, and it affects the overall productivity of lipid production. This has been a major bottleneck in commercializing the biofuels produced from microalgae.Among various strategies to address this bottleneck, metabolic engineering of N. oceanica to improve the lipid productivities has been the focus of this study. Efficient genetic tools that can generate knockouts and knock-ins are crucial for performing metabolic engineering in N. oceanica.The first part of this study is focussed on developing a genome editing toolbox for microalgae N. oceanica. To this end, we initially reviewed all the advancements made in developing a CRISPR-Cas based genetic toolbox in microalgae. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas) proteins are widely used across organisms to specifically target the genomic DNA and engineer the target region with high precision. We found that an approach based on transforming Cas ribonucleoproteins directly into model microalgae Chlamydomonas reinhardtii was a
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- 2021
31. Genetic engineering of microalgae for enhanced lipid production
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Wijffels, R.H., Weusthuis, R.A., D’ Adamo, S., Muñoz Segovia, Camilo F., Wijffels, R.H., Weusthuis, R.A., D’ Adamo, S., and Muñoz Segovia, Camilo F.
- Abstract
The global demand for food, feed and fossil-based chemicals is increasing due to the continuously growing world population. Excessive and unsustainable consumption is causing anthropogenic effects resulting in high carbon emission, pollution and depletion of natural resources. Currently, the main renewable feedstocks used as alternative to petrochemical processes are derived from agricultural crops. Higher plants are a great source of oils which can be used in food, feed, paint, lighting and fuel industries. However, cultivation of crops requires vast areas of arable land, fresh water and minerals. Additionally, depletion of petrochemical resources increases the demand for plant-based fuels, creating a direct competition with food and feed production. Therefore, novel renewable and sustainable alternatives are necessary to reduce the current environmental impact of traditional industrial processes.Microalgae are considered as a promising alternative for the production of commodities. They are capable of converting sunlight, water, CO2 and nutrients into biomass and products which attract interest from pharmaceutical, cosmetic, biofuel, food and feed industries. Furthermore, microalgae can reach significantly higher biomass concentrations and product yields per hectare compared to higher plants, they do not require arable lands and some species can be grown on wastewater or seawater.Several microalgal species can accumulate large amounts of fatty acids when cultivated under stress conditions. At a cellular level, lipids such as triacylglycerols (TAGs) and polyunsaturated fatty acids (PUFAs) can serve either as structural components or storage compounds. Therefore, microalgae are a source of valuable oils that can be used directly as nutritional products or as raw material for biofuel production.Although microalgae have the potential to become a feedstock for lipid production some limitations and challenges remain. In order to achieve an economically feasible process
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- 2021
32. Lipids and more : advancing Nannochloropsis as a biotechnological multipurpose chassis
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Barbosa, M.J., Wijffels, R.H., D’Adamo, S., Südfeld, Christian, Barbosa, M.J., Wijffels, R.H., D’Adamo, S., and Südfeld, Christian
- Abstract
Microalgae hold promise for sustainable production of commodities including food, feed, and green chemicals. Nannochloropsis oceanica is a particularly interesting candidate for industrial purposes and an emerging model organism for oleaginous. Although production of bulk commodities from N. oceanica is not yet economically feasible, strain improvement strategies could transform this microalga into a cell factory for cost-effective production of designer food, feed and bioproducts. This will require a comprehensive framework for metabolic engineering and synthetic biology as cornerstones to maximization of yields and productivities. These practices rely on a proper genome annotation, on an in-depth understanding of metabolic fluxes, and on a highly developed genetic manipulation toolset. Therefore, advancing our understanding of the metabolic network and expanding the toolbox for genetic engineering are of the utmost importance to establish N. oceanica as a commodity production platform.To improve the available tools for conducting forward genetic screens with N. oceanica, we developed a high-throughput screening for cellular neutral lipid content in chapter 2. Fluorescence activated cell sorting (FACS) was employed as a powerful single cell methodology. A robust and efficient neutral lipid staining procedure for N. oceanica was devised, utilizing the fluorophore BODIPY505/515. It was found that 6% DMSO, 1.2 µg ml-1 and 15 min staining duration are ideal for staining neutral lipids in exponentially growing N. oceanica cultures. For cultures that were stressed by nutrient deprivation, 10% DMSO, 1.2-1.6 µg ml-1 and 36 min duration were required. The combination of this staining method with FACS allowed quantitative prediction of cellular neutral lipid contents.Chapters 3-4 focused on developing novel tools for genetic manipulation of N. oceanica using CRISPR-Cas technology. Cas12a was employed as a signi
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- 2021
33. Central myonuclei and denervation markers in cancer cachexia
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Daou, N, Hassani, M, Matos, E, Salim De Castro, G, Figueredo Costa RG, Seelaender, M, Moresi, V, Rocchi, M, Adamo, S, Li, Z, Agbulut, O, and Coletti, D
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centrally located nuclei ,Myopathies ,cachexia - Published
- 2020
34. SRF-mediated mechanotransduction is essential for the response to exercise in cancer patients and animal models
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Hassani, M, Baccam, A, Benoni, A, Gargano, C, Salim de Castro, G, Alves, J, Chiappalupi, S, Moresi, V, Adamo, S, Riuzzi, F, Sorci, G, Muscaritoli, M, Seelaender, M, Xue, Z, Li, Z, Agbulut, O, and Coletti, D
- Published
- 2020
35. Peripheral Oxidative Stress Markers Are Related To Vascular Risk Factors And Subcortical Small Vessel Disease
- Author
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Warrick, N., Seitz, D., Prorok, J., Shawcross, D., Mahootchi, T., Esensoy, A., Yu, D., Danieli, E., Pushpakumar, D., Tony, J., Jacob, K., Dong, J., Javed, F., D’Souza, A., Mollayeva, T., Colantonio, A., Schulz, M., Burhan, A., Naidu, A. Srinivasan, Sarquis-Adamson, Y., Montero-Odasso, M., Cooper, N., Sekhon, H., Launay, C., Allali, G., Chabot, J., Beauchet, O., Watson, B., Lin, T., Korczak, A., Bartha, C., Best, S., Truemner, J., Borrie, M., Cammer, A., Whiting, S., Morgan, D., Newman, K., Duong, J. A., Mok, A., Wang, A. H., Lavoie, M., Bier, N., Macoir, J., Adlimoghaddam, A., Turner, R. S., Cadonic, C., Albensi, B. C., Davis, J., Lewis, V.-L., Pacione, J., Skanes, C., Feltz, N., Loncar, A., Naglie, G., Sanford, S., Stasiulis, E., Rapoport, M., Vrkljan, B., Tuokko, H., Porter, M., Polgar, J., Moorhouse, P., Mazer, B., Marshall, S., Gelinas, I, Crizzle, A, Belchior, P., Bedard, M, Kokorelias, K., Cameron, J., Gignac, M., Bechard, L., Beaton, D., McGilton, K.A., Tartaglia, M. C., Black, S., Mirza, S., Mutsaerts, H.-J., Cash, D., Bocchetta, M., Thomas, D., Dick, K., van Swieten, J., Borroni, B., Galimberti, D., Rowe, J., Bethell, J., Pringle, D., Commisso, E., Chambers, L., Cohen, C., Cowan, K., Fehr, P., Szeto, P., McGilton, K., Shaw, C., Okamura, H., Otani, M., Shimoyama, N., Fujii, T., Lusk, J., Punzalan, M., Dove, E., Cotnam, K., Astell, A., Chow, A. Froehlich, Bayly, M., Kosteniuk, J., Elliot, V., O’Connell, M. E., Kirk, A., Stewart, N., Holroyd-Leduc, J., Daku, J., Kennett-Russill, D., Hack, T., Dilara, A., Astell, A. J., Hernandez, A., Divine, A., Hunter, S., Jacova, C., Alexander, C., Joseph, J. T., Alvarez, A., Smith, E., Woo, S. M. S., Chan, P., Wilkins-Ho, M., Blackburn, P., Fernando, N., Mehra, A., Vasser, E., Musacchio, M., Waxman, R., Fischler, I., Ghaffar, O., DeBay, D. R., Macdonald, I. R., Reid, G. A., Pottie, I. R., Maxwell, S. P., Cash, M. K., Martin, E., Bowen, C. V., Darvesh, S., MacPhee, J., Jorgensen, M., Fogarty, J., Phillips, N., Diprospero, C., Parent, A., Whitehead, V., Campbell, T., Mohades, Z., Chertkow, H., Wong, S., Wilchesky, M., McCusker, J., Champoux, N., Vu, T.T. M., Ciampi, A., Monette, J., Lungu, O., Ballard, S. A., Belzile, E., Carmichael, P.-H., Voyer, P., Cetin-Sahin, D., Gore, B., Peretti, M., Gore, G., Landry, V., Yetman, L., MacDonald, E., McGibbon, C., MacNeil, D., Jarrett, P., Iaboni, A., Andrews, J., Hafezi, S., Marshall, C., Tsokas, M., Martin, L. Schindel, Van Ooteghem, K., Mansfield, A., Marcil, M., Gold, D., Musselman, K., Flint, A., Finger, E., Feldman, H., Cummings, J., Coleman, K., Boxer, A., Berry, S., Hsiung, R., Curtis, A., Zhang, K., Davidson, H. R., Boccone, G., Camicioli, R., Masellis, M., Tierney, M., Dolatabadi, E., Taati, B., Jonas-Simpson, C., Donovan, L., Cross, N., Keren, R., Shan, R., Holley, J., Waisman, Z., Katchaluba, J., Wimhurst, C., Steele, M., Loganathan, P., Gural, P., Shearer, T., Reardon, J., Pilgrim, J., Pitawanakwat, K., Jones, L., Piriano, E., Blind, M., Otowadjiwan, J., Makela, R., Spicer, B., Bretzlaff, M., Jacklin, K., McKay, Kristy, Graham, N., Tang-Wai, D., Leonard, C., Mitchell, S., Laird, L., Rochon, E., Maclagan, L., Maxwell, C., Guan, J., Campitelli, M., Herrmann, N., Lapane, K., Hogan, D., Amuah, J., Gill, S., Bronskill, S., Ebert, P., Kwok, J., Watt, A., Garrett, S., Hoefling, L., Ellery, C., Leggieri, M., Fornazzari, L., Thaut, M., Munoz, D., Barfett, J., Fischer, C., Schweizer, T., Yogaparan, T., Dallaire-Théroux, C., Potvin, O., Dieumegarde, L., Duchesne, Simon, Amini, A.E. Ebrahim, Amini, A.Z. Ebrahim, Dao, E., Barha, C. K., Best, J. R., Hsiung, G.-Y. R., Tam, R., Liu-Ambrose, T., Sztramko, R., Wurster, A., Papaiouannou, A., Cowan, D., St. Onge, J., Allaby, C., Harrison, L., Cimino, C., Marr, S., Patterson, C., Woo, T., Levinson, A., Fisher, S., Mojaverian, N., Hsu, A., Taljaard, M., Manuel, D., Tanuseputro, P., Park, E., Liu, L., VanderPloeg, K., Black, A., Bartha, R., Rabin, J., Yang, H.-S., Schultz, A., Hanseeuw, B., Marshall, G., Hedden, T., Rentz, D., Johnson, K., Sperling, R., Chhatwal, J., Desmarais, P., Miville, C., Keith, J., Lanctôt, K., Thomas, N., Mattek, N., Riley, T., Witter, P., Reynolds, C., Austin, J., Sharma, N., Kaye, J., Bechard, L. E., Mitchell, C. M., Regan, K., Bergelt, M. D., Middleton, L.E., Hewston, P., Kennedy, C., Merom, D., Trainor, L., Grenier, A., Ioannidis, G., Lee, J., Papaioannou, A., Qian, W., Churchill, N., Kumar, S., Rajji, T., Ojeda-López, C., Milán-Tomás, Á., Lam, B., Gao, F. Q., Cumberbatch, S., Gies, S., Tomas, A. Milan, Ojeda-Lopez, C., Lim, A. S., Black, S. E., Sharma, M. J., Ramirez, J., Holmes, M. F., Gao, F., Varatharajah, B., Yhap, V., Appel, L., Bogler, O., Appel, E., Wiseman, M., Cohen, L., Hill, D., Abrams, H., Campos, J., Sapkota, S., Adamo, S., Stuss, D. T., Martinez, M., Multani, N., Anor, C. J., Fox, S., Lang, A. E., Marras, C., Compagnone, J., Li, J., Freedman, M., Kleiner-Fisman, G., Kennedy, J., Chen, R., Lang, A., Sévigny-Dupont, P., Bocti, C., Joannette, M., Lavallée, M. M., Joubert, S., Knoefel, F., Goubran, R., Baker, A., Fraser, S., Allard, B., Wallace, B., Stroulia, E., Guana, V., Masson, P., Alli, S., Kolla, N., De Luca, V., Bouvier, L., Monetta, L., Vitali, P., Laforce, R., Martel-Sauvageau, V., Talebzadeh, A., Ashourinia, K., Moy, S., Lake, A., Cockburn, A., Krisman, D., Sadasivan, B., Sit, W., Stoops, S., McCurbin, S., Cullen, S., Carroll, S., Tasmim, S., Kapoor, E., Callahan, B., Sharma, M., Bierstone, D., Stuss, D., Kapadia, M., Mian, F., Ma, D., Rosa, E., Michalski, B., Zovkic, I., Forsythe, P., Sakic, B., Fahnestock, M., Baxter, J., Peloso, S., Tung, J., Cox, L., Benjamin, S., An, H., Ho, J., Turcotte, V., Parent, C., Gauthier-Beaupré, A., Biss, R., Sultana, A., Chu, C. H., Sun, W., Bartfay, E., Smye, V., Newton, D., Pepin, M., Biswas, S., Madahey, H., Crawford, S. J., Gutmanis, I., Blake, C., Duchesne, S., Hudon, C., Mah, L., Ali, A., Shorey, C., Szabuniewicz, C. M., Anderson, N. D., Verhoeff, N. P. L. G., Cheers, S., Penko, M., Gevaert, V., Yang, Y., Law, J., Modarresi, S., Grahn, J., Overend, T., Amini, D., Thiruparanathan, T., Cheung, T., Iskandar, S., Arone, Y., Young, C., Berezuk, C., and Zakzanis, K.
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Abstracts - Published
- 2018
36. Erratum to: What to Do, and What Not to Do, When Diagnosing and Treating Breakthrough Cancer Pain (BTcP): Expert Opinion
- Author
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Vellucci, R., Fanelli, G., Pannuti, R., Peruselli, C., Adamo, S., Alongi, G., Amato, F., Consoletti, L., Lamarca, L., Liguori, S., Presti, C. Lo, Maione, A., Mameli, S., Marinangeli, F., Marulli, S., Minotti, V., Miotti, D., Montanari, L., Moruzzi, G., Palermo, S., Parolini, M., Poli, P., Tirelli, W., Valle, A., Romualdi, P., and Working Group Nientemale DEI
- Published
- 2016
- Full Text
- View/download PDF
37. Correlation between MOSFETs breakdown and 4H-SiC epitaxial defects
- Author
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Fiorenza, P., primary, Adamo, S., additional, Alessandrino, M. S., additional, Bottari, C., additional, Carbone, B., additional, Di Martino, C., additional, Russo, A., additional, Saggio, M., additional, Venuto, C., additional, Vitanza, E., additional, Zanetti, E., additional, Giannazzo, F., additional, and Roccaforte, F., additional
- Published
- 2021
- Full Text
- View/download PDF
38. Intrinsic Gating Behavior of Voltage-Gated Sodium Channels Predetermines Regulation by Auxiliary β-subunits
- Author
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Brake, Niklas, primary, Mancino, Adamo S, additional, Yan, Yuhao, additional, Shimomura, Takushi, additional, Silveira, Heika, additional, Kubo, Yoshihiro, additional, Khadra, Anmar, additional, and Bowie, Derek, additional
- Published
- 2021
- Full Text
- View/download PDF
39. Surgical results and functional outcome after total anorectal reconstruction by double graciloplasty supported by external-source electrostimulation and/or implantable pulse generators: an 8-year experience
- Author
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Violi, Vincenzo, Boselli, Adamo S., De Bernardinis, Massimo, Costi, Renato, Nervi, Giorgio, Bertelè, Anna, Franzè, Angelo, and Roncoroni, Luigi
- Published
- 2004
- Full Text
- View/download PDF
40. 3-Hydroxytyrosol protects human chondrocytes against cell death and matrix degradation: SW03.S13–75
- Author
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Cetrullo, S., Facchini, A., DʼAdamo, S., Tantini, B., Borzi, R. M., Pignatti, C., and Flamigni, F.
- Published
- 2013
41. Functionalization of decellularized scaffold for skeletal muscle tissue engineering: OP-003
- Author
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Costa, A, Aprile, P, Aulino, P, Rossi, D, Perniconi, B, Adamo, S, Coletti, D, and Teodori, L
- Published
- 2013
42. A patient-rated, surgeon-corrected scale for functional assessment after total anorectal reconstruction: An adaptation of the Working Party on Anal Sphincter Replacement scoring system
- Author
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Violi, Vincenzo, Boselli, Adamo S., De Bernardinis, Massimo, Costi, Renato, Trivelli, Marina, and Roncoroni, Luigi
- Published
- 2002
- Full Text
- View/download PDF
43. Rectal cancer in anorectal malformation with rectovestibular fistula: Sphincter-restoring surgery by anterior resection and dynamic vaginoanograciloplasty
- Author
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Violi, Vincenzo, Boselli, Adamo S., Sarli, Leopoldo, Costi, Renato, and Roncoroni, Luigi
- Published
- 2001
- Full Text
- View/download PDF
44. CLIMATE CHANGE: Preparing for Resettlement Associated with Climate Change
- Author
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de Sherbinin, A., Castro, M., Gemenne, F., Cernea, M. M., Adamo, S., Fearnside, P. M., Krieger, G., Lahmani, S., Oliver-Smith, A., Pankhurst, A., Scudder, T., Singer, B., Tan, Y., Wannier, G., Boncour, P., Ehrhart, C., Hugo, G., Pandey, B., and Shi, G.
- Published
- 2011
- Full Text
- View/download PDF
45. Procedimento per la sintesi di esosomi contenenti proteine da shock termico e loro uso per il trattamento della atrofia muscolare e della cachessia
- Author
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Di Felice V., Farina F., Cappello F., Campanella C., Barone R., Marino Gammazza A., Trovato E., D'Amico D., Macaluso F., Coletti D., Adamo S., Multhoff G., Gasco P., and Di Felice V., Farina F., Cappello F., Campanella C., Barone R., Marino Gammazza A., Trovato E., D'Amico D., Macaluso F., Coletti D., Adamo S., Multhoff G., Gasco P.
- Subjects
Settore BIO/16 - Anatomia Umana ,cachexia, skelethal muscle, exosomes, extracellular vescicles, Hsp60 - Published
- 2018
46. Le valutazioni di bilancio nella prospettiva della tradizione giuridica e dottrinale italiana
- Author
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Adamo, S, Costa, M, Stefano Adamo, Anna Maria Fellegara, Alberto Incollingo, Andrea Lionzo, Adamo, Stefano, Costa, Massimo, Adamo, S, Fellegara, AM, Incollingo, A, Lionzo, A, Costa, M, Aversano, N, Sannino, G, Tartaglia Polcini, P, Quagli, A, Cosentino, A, Coluccia, D, Fontana, S, Giornetti, A, Moscarini, F, Solimene, S, Sura, A, Maglio, R, Agliata, F, Marinoni, MA, Rey, A, Giaccari, F, Magli, F, Ogliari, M, Braja, EM, Campra, M, Maffei, M, Mari, LM, Terzani, S, Busso, D, Marcon, C, Pucci, S, Venuti, M, Agostini, M, Capodaglio, G, Dangarska, V, Devalle, A, Fradeani, A, Lucchese, M, Rizzato, F, Semprini, L, Sostero, U, Turco, M, Papa, M, Spallini, S, Azzali, S, Fornaciari, L, Mazza, T, Pierotti, MR, Bava, F, Doni, F, Fasiello, R, Giovando, G, Teodori, C, Carini, C, and Veneziani, M
- Subjects
Bilancio, Dottrina contabile, Valutazioni, Ragioneria ,Settore SECS-P/07 - Economia Aziendale ,valutazioni di bilancio, dottrina aziendale, literature review - Abstract
Il capitolo si inserisce quale introduzione storiografica al tema oggetto di una monografia open source patrocinata dalla Sidrea (Società Italiana di Ragioneria ed Economia Aziendale). Detta monografia è il frutto di una ricerca collettanea sulla riforma del bilancio civilistico ex DPR 139/2015. I singoli contributi della stessa riguardano infatti le singole aree del bilancio. Rispetto a questa complessiva ricerca della società accademica di settore, il saggio/capitolo in oggetto, a natura esplicitamente introduttiva, intende mostrare come la disciplina giuridica sia sempre stata influenzata da un dibattito dottrinale a monte di tipo economico-aziendale, questo in Italia particolarmente ricco. Esso, quindi, ripercorre questa storia del pensiero, con particolare riferimento ai temi delle valutazioni di bilancio, a partire dai pioneristici contributi del XVI/XVII secolo, a seguire con le Scuole Classiche ottocentesche di Ragioneria, a sviluppare i contributi dei più grandi Maestri del Novecento, fino ad approdare alle più recenti tendenze di dottrina e di ricerca in materia di valutazioni nel nostro Paese. In parallelo alla suddetta evoluzione dottrinaria è presentata criticamente l’evoluzione della normativa civile/commerciale, di cui il DPR 139/2015 rappresenta solo l’ultima pagina di storia: da qualche cenno alle regolamentazioni pre-unitarie, al codice di commercio del 1865, a quello del 1882, al codice civile del 1942, alla L.216/1974, al D. L.vo 127/1991 e via via agli altri interventi sino all’ultima novella oggetto dell’intera monografia.
- Published
- 2018
47. The mechanical stimulation of myotubes counteracts the effects of tumor-derived factors through IL-4 secretion and the modulation of the activin/follistatin ratio
- Author
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Coletti, D, Baccam, A, Benoni, A, Rocchi, M, Moresi, V, Seelaender, M, Li, Z, Gargano, C, Adamo, S, and Xue, Z.
- Subjects
myokines ,oxytocin ,skeletal muscle - Published
- 2019
48. Elementi di istologia e cenni di embriologia
- Author
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Adamo, S., Bernardini, N., Boitani, C., Bonsi, L., Bouché, M., Brun, P., Canipari, R., Castriconi, R., Castrogiovanni, P., Ciccarelli, C., DE ANGELIS, L., D’Alessio, A., DE CESARIS, P., DE FELICI, M., DE MATTEI, M., Desiderio, V., DI ROSA, M., Dolfi, A., Dupont, S., Fazi, F., Filippini, A., Gagliano, N., Grano, M., Imbesi, R., Marcenaro, E., Musarò, A., Nervi, C., Papaccio, G. P., Ricci, G., Riccioli, A., Salustri, A., Scicchitano, B. M., Sivori, S., Tirino, V., Vicini, E., Virgintino, D., and Ziparo, E.
- Published
- 2019
49. The Mechanical Stimulation of Myotubes Counteracts the Effects of Tumor-Derived Factors Through the Modulation of the Activin/Follistatin Ratio
- Author
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Baccam, A, Benoni-Sviercovich, A, Rocchi, M, Moresi, V, Seelaender, M, Li, Z, Adamo, S, Xue, Z, Coletti, D, Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), and Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,skeletal muscle atrophy ,exercise ,C26 colon carcinoma ,FlexCell apparatus ,mechanotransduction ,myokines ,[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2019
50. SRF role as a mechano-transductor in response to exercise in cancer cachexia
- Author
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Hassani, M, Benoni, A, Xue, Z, Sotiropoulos, A, Parlakian, A, Li, Z, Adamo, S, and Coletti, D
- Subjects
exercise ,muscle wasting ,cachexia ,skeletal muscle - Published
- 2019
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