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The cell wall (CW) of plant-interacting fungi, as the direct interface with host plants, plays a crucial role in fungal development. A number of secreted proteins are directly associated with the fungal CW, either through covalent or non-covalent interactions, and serve a range of important functions. In the context of plant-fungal interactions many are important for fungal development in the host environment and may therefore be considered fungal CW-associated effectors (CWAEs). Key CWAE functions include integrating chemical/physical signals to direct hyphal growth, interfering with plant immunity, and providing protection against plant defenses. In recent years, a diverse range of mechanisms have been reported that underpin their roles, with some CWAEs harboring conserved motifs or functional domains, while others are reported to have novel features. As such, the current understanding regarding fungal CWAEs is systematically presented here from the perspective of their biological functions in plant-fungal interactions. An overview of the fungal CW architecture and the mechanisms by which proteins are secreted, modified, and incorporated into the CW is first presented to provide context for their biological roles. Some CWAE functions are reported across a broad range of pathosystems or symbiotic/mutualistic associations. Prominent are the chitin interacting-effectors that facilitate fungal CW modification, protection, or suppression of host immune responses. However, several alternative functions are now reported and are presented and discussed. CWAEs can play diverse roles, some possibly unique to fungal lineages and others conserved across a broad range of plant-interacting fungi. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.

We report the distribution of black hole (BH) masses and Eddingont ratios estimated for a sample of 131 low luminosity quasars in the early cosmic epoch (5.6 < z < 7.0). Our work is based on the Subaru High-z Exploration of Low-Luminosity Quasars (SHELLQs) project, which has constructed a low luminosity quasar sample down to M1450 ∼ − 21 mag, exploiting the survey data of Hyper Suprime-Cam installed on Subaru Telescope. The discovery spectra of these quasars are limited to the rest-frame wavelengths of ∼1200–1400 Å, which contain no emission lines that can be used as BH mass estimators. In order to overcome this problem, we made use of low-z counterpart spectra from the Sloan Digital Sky Survey, which are spectrally matched to the high-z spectra in overlapping wavelengths. We then combined the C iv emission line widths of the counterparts with the continuum luminosity from the SHELLQs data to estimate BH masses. The resulting BH mass distribution has a range of ∼107–10M⊙, with most of the quasars having BH masses ∼108M⊙ with sub-Eddington accretion. The present study provides not only a new insight into normal quasars in the reionization epoch, but also a new promising way to estimate BH masses of high-z quasars without near-infrared spectroscopy.

The persistence of planetary systems after their host stars evolve into their post-main-sequence phase is poorly constrained by observations. Many young white dwarf systems exhibit infrared excess emission and/or spectral absorption lines associated with a reservoir of dust (or planetesimals) and its accretion. However, most white dwarfs are too cool to sufficiently heat any circumstellar dust to detectable levels of emission. The Helix Nebula (NGC 7293) is a young, nearby planetary nebula; observations at mid- and far-infrared wavelengths have revealed excess emission associated with its central white dwarf (WD 2226-210). The origin of this excess is ambiguous. It could be a remnant planetesimal belt, a cloud of comets, or the remnants of material shed during the post-asymptotic giant branch (post-AGB) phase. Here we combine infrared (Stratospheric Observatory for Infrared Astronomy, Spitzer, Herschel) and millimeter (Atacama Large Millimeter/submillimeter Array) observations of the system to determine the origin of this excess using multiwavelength imaging and radiative transfer modeling. We find the data are incompatible with a compact remnant planetesimal belt or post-AGB disk, and conclude the dust most likely originates from deposition by a cometary cloud. The measured dust mass, and lifetime of the constituent grains, implies disruption of several thousand Hale–Bopp equivalent comets per year to fuel the observed excess emission around the Helix Nebula's white dwarf.

Since SARM1 mutations have been identified in human neurological disease, SARM1 inhibition has become an attractive therapeutic strategy to preserve axons in a variety of disorders of the peripheral (PNS) and central nervous system (CNS). While SARM1 has been extensively studied in neurons, it remains unknown whether SARM1 is present and functional in myelinating glia? This is an important question to address. Firstly, to identify whether SARM1 dysfunction in other cell types in the nervous system may contribute to neuropathology in SARM1 dependent diseases? Secondly, to ascertain whether therapies altering SARM1 function may have unintended deleterious impacts on PNS or CNS myelination? Surprisingly, we find that oligodendrocytes express sarm1 mRNA in the zebrafish spinal cord and that SARM1 protein is readily detectable in rodent oligodendrocytes in vitro and in vivo. Furthermore, activation of endogenous SARM1 in cultured oligodendrocytes induces rapid cell death. In contrast, in peripheral glia, SARM1 protein is not detectable in Schwann cells and satellite glia in vivo and sarm1/Sarm1 mRNA is detected at very low levels in Schwann cells, in vivo, in zebrafish and mouse. Application of specific SARM1 activators to cultured mouse Schwann cells does not induce cell death and nicotinamide adenine dinucleotide (NAD) levels remain unaltered suggesting Schwann cells likely contain no functionally relevant levels of SARM1. Finally, we address the question of whether SARM1 is required for myelination or myelin maintenance. In the zebrafish and mouse PNS and CNS, we show that SARM1 is not required for initiation of myelination and myelin sheath maintenance is unaffected in the adult mouse nervous system. Thus, strategies to inhibit SARM1 function to treat neurological disease are unlikely to perturb myelination in humans.

We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these questions include the need to better understand how plants detect water availability, temperature, salinity, and rising carbon dioxide (CO2) levels; how environmental signals interface with endogenous signaling and development (e.g. circadian clock and flowering time); and how this integrated signaling controls downstream responses (e.g. stomatal regulation, proline metabolism, and growth versus defense balance). The plasma membrane comes up frequently as a site of key signaling and transport events (e.g. mechanosensing and lipid-derived signaling, aquaporins). Adaptation to water extremes and rising CO2 affects hydraulic architecture and transpiration, as well as root and shoot growth and morphology, in ways not fully understood. Environmental adaptation involves tradeoffs that limit ecological distribution and crop resilience in the face of changing and increasingly unpredictable environments. Exploration of plant diversity within and among species can help us know which of these tradeoffs represent fundamental limits and which ones can be circumvented by bringing new trait combinations together. Better defining what constitutes beneficial stress resistance in different contexts and making connections between genes and phenotypes, and between laboratory and field observations, are overarching challenges.

Glycolipids with TLR4 agonistic properties can serve either as therapeutic agents or as vaccine adjuvants by stimulating the development of proinflammatory responses. Translating them to the clinical setting is hampered by synthetic difficulties, the lack of stability in biological media, and/or a suboptimal profile of balanced immune mediator secretion. Here, we show that replacement of the sugar fragment by an sp2-iminosugar moiety in a prototypic TLR4 agonist, CCL-34, yields iminoglycolipid analogues that retain or improve their biological activity in vitro and in vivo and can be accessed through scalable protocols with total stereoselectivity. Their adjuvant potential is manifested in their ability to induce the secretion of proinflammatory cytokines, prime the maturation of dendritic cells, and promote the proliferation of CD8+ T cells, pertaining to a Th1-biased profile. Additionally, their therapeutic potential for the treatment of asthma, a Th2-dominated inflammatory pathology, has been confirmed in an ovalbumin-induced airway hyperreactivity mouse model.

The HST treasury programme BUFFALO provides extended wide-field imaging of the six Hubble Frontier Fields galaxy clusters. Here we present the combined strong and weak-lensing analysis of Abell 370, a massive cluster at z = 0.375. From the reconstructed total projected mass distribution in the 6 arcmin × 6 arcmin BUFFALO field-of-view, we obtain the distribution of massive substructures outside the cluster core and report the presence of a total of seven candidates, each with mass ∼5 × 1013 M⊙. Combining the total mass distribution derived from lensing with multiwavelength data, we evaluate the physical significance of each candidate substructure, and conclude that five out of the seven substructure candidates seem reliable, and that the mass distribution in Abell 370 is extended along the north-west and south-east directions. While this finding is in general agreement with previous studies, our detailed spatial reconstruction provides new insights into the complex mass distribution at large cluster-centric radius. We explore the impact of the extended mass reconstruction on the model of the cluster core and in particular, we attempt to physically explain the presence of an important external shear component, necessary to obtain a low root-mean-square separation between the model-predicted and observed positions of the multiple images in the cluster core. The substructures can only account for up to half the amplitude of the external shear, suggesting that more effort is needed to fully replace it by more physically motivated mass components. We provide public access to all the lensing data used as well as the different lens models.

[Summary] Extensive adenosine-to-inosine (A-to-I) editing of nuclear-transcribed mRNAs is the hallmark of metazoan transcriptional regulation. Here, by profiling the RNA editomes of 22 species that cover major groups of Holozoa, we provide substantial evidence supporting A-to-I mRNA editing as a regulatory innovation originating in the last common ancestor of extant metazoans. This ancient biochemistry process is preserved in most extant metazoan phyla and primarily targets endogenous double-stranded RNA (dsRNA) formed by evolutionarily young repeats. We also find intermolecular pairing of sense-antisense transcripts as an important mechanism for forming dsRNA substrates for A-to-I editing in some but not all lineages. Likewise, recoding editing is rarely shared across lineages but preferentially targets genes involved in neural and cytoskeleton systems in bilaterians. We conclude that metazoan A-to-I editing might first emerge as a safeguard mechanism against repeat-derived dsRNA and was later co-opted into diverse biological processes due to its mutagenic nature.

[Background] Accurate and comprehensive annotation of transcript sequences is essential for transcript quantification and differential gene and transcript expression analysis. Single-molecule long-read sequencing technologies provide improved integrity of transcript structures including alternative splicing, and transcription start and polyadenylation sites. However, accuracy is significantly affected by sequencing errors, mRNA degradation, or incomplete cDNA synthesis., [Results] We present a new and comprehensive Arabidopsis thaliana Reference Transcript Dataset 3 (AtRTD3). AtRTD3 contains over 169,000 transcripts—twice that of the best current Arabidopsis transcriptome and including over 1500 novel genes. Seventy-eight percent of transcripts are from Iso-seq with accurately defined splice junctions and transcription start and end sites. We develop novel methods to determine splice junctions and transcription start and end sites accurately. Mismatch profiles around splice junctions provide a powerful feature to distinguish correct splice junctions and remove false splice junctions. Stratified approaches identify high-confidence transcription start and end sites and remove fragmentary transcripts due to degradation. AtRTD3 is a major improvement over existing transcriptomes as demonstrated by analysis of an Arabidopsis cold response RNA-seq time-series. AtRTD3 provides higher resolution of transcript expression profiling and identifies cold-induced differential transcription start and polyadenylation site usage., [Conclusions] AtRTD3 is the most comprehensive Arabidopsis transcriptome currently. It improves the precision of differential gene and transcript expression, differential alternative splicing, and transcription start/end site usage analysis from RNA-seq data. The novel methods for identifying accurate splice junctions and transcription start/end sites are widely applicable and will improve single-molecule sequencing analysis from any species.

The ALMA (Atacama Large Millimeter Array) interferometer, with its unprecedented combination of high sensitivity and high angular resolution, allows for (sub-)mm wavelength mapping of protostellar systems at Solar system scales. Astrochemistry has benefitted from imaging interstellar complex organic molecules in these jet–disc systems. Here, we report the first detection of methanol (CH3OH) and methyl formate (HCOOCH3) emission towards the triple protostellar system VLA1623−2417 A1+A2+B, obtained in the context of the ALMA Large Programme FAUST (Fifty AU STudy of the chemistry in the disc/envelope system of solar-like protostars). Compact methanol emission is detected in lines from Eu = 45 K up to 61 K and 537 K towards components A1 and B, respectively. Large velocity gradient analysis of the CH3OH lines towards VLA1623−2417 B indicates a size of 0.11–0.34 arcsec (14–45 au), a column density NCH3OH = 1016–1017 cm−2, kinetic temperature ≥ 170 K, and volume density ≥ 108 cm−3. A local thermodynamic equilibrium approach is used for VLA1623−2417 A1, given the limited Eu range, and yields Trot ≤ 135 K. The methanol emission around both VLA1623−2417 A1 and B shows velocity gradients along the main axis of each disc. Although the axial geometry of the two discs is similar, the observed velocity gradients are reversed. The CH3OH spectra from B show two broad (4–5 km s−1) peaks, which are red- and blueshifted by ∼ 6–7 km s−1 from the systemic velocity. Assuming a chemically enriched ring within the accretion disc, close to the centrifugal barrier, its radius is calculated to be 33 au. The methanol spectra towards A1 are somewhat narrower (∼ 4 km s−1), implying a radius of 12–24 au.

The ATP binding sites of many enzymes are structurally related, which complicates their development as therapeutic targets. In this work, we explore a diverse set of ATPases and compare their ATP binding pockets using different strategies, including direct and indirect structural methods, in search of pockets attractive for drug discovery. We pursue different direct and indirect structural strategies, as well as ligandability assessments to help guide target selection. The analyses indicate human RAD51, an enzyme crucial in homologous recombination, as a promising, tractable target. Inhibition of RAD51 has shown promise in the treatment of certain cancers but more potent inhibitors are needed. Thus, we design compounds computationally against the ATP binding pocket of RAD51 with consideration of multiple criteria, including predicted specificity, drug-likeness, and toxicity. The molecules designed are evaluated experimentally using molecular and cell-based assays. Our results provide two novel hit compounds against RAD51 and illustrate a computational pipeline to design new inhibitors against ATPases.

Vega is the prototypical debris disc system. Its architecture has been extensively studied at optical to millimetre wavelengths, revealing a near face-on, broad, and smooth disc with multiple distinct components. Recent millimetre-wavelength observations from ALMA spatially resolved the inner edge of the outer, cold planetesimal belt from the star for the first time. Here we present early science imaging observations of the Vega system with the AzTEC instrument on the 32-m LMT, tracing extended emission from the disc out to 150 au from the star. We compare the observations to three models of the planetesimal belt architecture to better determine the profile of the outer belt. A comparison of these potential architectures for the disc does not significantly differentiate between them with the modelling results being similar in many respects to the previous ALMA analysis, but differing in the slope of the outer region of the disc. The measured flux densities are consistent between the LMT (single dish) and ALMA (interferometric) observations after accounting for the differences in wavelength of observation. The LMT observations suggest the outer slope of the planetesimal belt is steeper than was suggested in the ALMA analysis. This would be consistent with the interferometric observations being mostly blind to structure at the disc outer edges, but the overall low signal to noise of the LMT observations does not definitively resolve the structure of the outer planetesimal belt.

Co-immunoprecipitation (Co-IP) is a widely used and powerful approach for studying protein-protein interactions in vivo. Here, we describe a protocol for antibody purification and immobilization followed by immunoprecipitation from plant tissue extracts using magnetic beads. The protocol has been used to detect regulators in the Zea mays phenylpropanoid pathway. The protocol is amenable to a variety of downstream assays, including western blotting and mass spectrometry. For complete details on the use and execution of this protocol, please refer to Vélez-Bermúdez et al. (2015).

Protostellar outflows and jets play a vital role in star formation as they carry away excess angular momentum from the inner disk surface, allowing the material to be transferred toward the central protostar. Theoretically, low-velocity and poorly collimated outflows appear from the beginning of the collapse at the first hydrostatic core (FHSC) stage. With growing protostellar core mass, high-density jets are launched, entraining an outflow from the infalling envelope. Until now, molecular jets have been observed at high velocity (≳100 km s) in early Class 0 protostars. We, for the first time, detect a dense molecular jet in SiO emission with low velocity (∼4.2 km s, deprojected ∼24 km s) from source G208.89-20.04Walma (hereafter G208Walma) using ALMA Band 6 observations. This object has some characteristics of FHSCs, such as a small outflow/jet velocity, extended 1.3 mm continuum emission, and N D line emission. Additional characteristics, however, are typical of early protostars: collimated outflow and SiO jet. The full extent of the outflow corresponds to a dynamical timescale of ∼ 930 − 100 + 200 yr. The spectral energy distribution also suggests a very young source having an upper limit of T ∼ 31 K and L ∼ 0.8 L . We conclude that G208Walma is likely in the transition phase from FHSC to protostar, and the molecular jet has been launched within a few hundred years of initial collapse. Therefore, G208Walma may be the earliest object discovered in the protostellar phase with a molecular jet.

Although first considered as too diluted for the formation of molecules in-situ and too harsh an environment for their survival, the interstellar medium has turned out to host a rich palette of molecular species: to date, 256 species, not counting isotopologues, have been identified. The last decade, and more particularly the last 2 years, have seen an explosion of new detections, including those of a number of complex organic species, which may be dubbed as prebiotic. Organic molecules have been discovered not just in interstellar clouds from the Solar neighbourhood, but also throughout the Milky-Way, as well as in nearby galaxies, or some of the most distant quasars. These discoveries were made possible by the completion of large sub-millimetre and radio facilities. Equipped with new generation receivers, those instruments have provided the orders of magnitude leap in sensitivity required to detect the vanishingly weak rotational lines that allowed the molecule identifications. Last 2 years, 30 prebiotic molecules have been detected in TMC-1, a dustenshrouded gaseous cloud located at 400 light-years from the Sun in the Taurus constellation. Ten new molecular species, have been identified in the arm of a spiral galaxy seven billion light-yr distant, and 12 molecular species observed in a quasar at 11 billion light-yr. We present the latest spectral observations of this outlying quasar and discuss the implications of those detections in these 3 archetypal sources. The basic ingredients involved in the Miller-Urey experiment and related experiments (H2, H2O, CH4, NH3, CO, H2S, ... ) appeared early after the formation of the first galaxies and are widespread throughout the Universe. The chemical composition of the gas in distant galaxies seems not much different from that in the nearby interstellar clouds. It presumably comprises, like for TMC-1, aromatic rings and complex organic molecules putative precursors of the RNA nucleobases, except the lines of such complex s

The transition from the gabbroic oceanic crust to the residual mantle harzburgites of the Oman ophiolite has been drilled at Holes CM1A and CM2B (Wadi Tayin massif) during Phase 2 of the International Continental Scientific Drilling Program Oman Drilling Project (November 2017–January 2018). In order to unravel the formation processes of ultramafic rocks in the Wadi Tayin massif crust-mantle transition zone and deeper in the mantle sections beneath oceanic spreading centers, our study focuses on the whole rock major and trace element compositions (together with CO and HO concentrations) of these ultramafic rocks (56 dunites and 49 harzburgites). Despite extensive serpentinization and some carbonation, most of the trace element contents (REE, HFSE, Ti, Th, U) record high temperature, magmatic process-related signatures. Two major trends are observed, with good correlations between (a) Th and U, Nb and LREE on one hand, and between (b) heavy REE, Ti and Hf on the other hand. We interpret the first trend as the signature of late melt/peridotite interactions as LREE are known to be mobilized by such processes (‘‘lithospheric process’’) and the second trend as the signature of the initial mantle partial melting (‘‘asthenospheric process’’), with little or no overprint from melt/rock reaction events.

Context. Future astrophysical surveys such as J-PAS will produce very large datasets, the so-called "big data", which will require the deployment of accurate and efficient machine-learning (ML) methods. In this work, we analyze the miniJPAS survey, which observed about similar to 1 deg(2) of the AEGIS field with 56 narrow-band filters and 4 ugri broad-band filters. The miniJPAS primary catalog contains approximately 64 000 objects in the r detection band (mag(AB)less than or similar to 24), with forced-photometry in all other filters.Aims. We discuss the classification of miniJPAS sources into extended (galaxies) and point-like (e.g., stars) objects, which is a step required for the subsequent scientific analyses. We aim at developing an ML classifier that is complementary to traditional tools that are based on explicit modeling. In particular, our goal is to release a value-added catalog with our best classification.Methods. In order to train and test our classifiers, we cross-matched the miniJPAS dataset with SDSS and HSC-SSP data, whose classification is trustworthy within the intervals 15 <= r <= 20 and 18.5 <= r <= 23.5, respectively. We trained and tested six different ML algorithms on the two cross-matched catalogs: K-nearest neighbors, decision trees, random forest (RF), artificial neural networks, extremely randomized trees (ERT), and an ensemble classifier. This last is a hybrid algorithm that combines artificial neural networks and RF with the J-PAS stellar and galactic loci classifier. As input for the ML algorithms we used the magnitudes from the 60 filters together with their errors, with and without the morphological parameters. We also used the mean point spread function in the r detection band for each pointing.Results. We find that the RF and ERT algorithms perform best in all scenarios. When the full magnitude range of 15 <= r <= 23.5 is analyzed, we find an area under the curve AUC=0.957 with RF when photometric information alone is used, and AUC

Aims. We investigate the structure, dynamics, and chemistry of the molecule-rich nebula around the stellar symbiotic system R Aqr, which is significantly affected by the presence of a white dwarf (WD) companion. We study the effects of the strong dynamical interaction between the AGB wind and the WD and of photodissociation by the WD UV radiation on the circumstellar shells. Methods. We obtained high-quality ALMA maps of the 12CO J = 2-1, J = 3-2, and J = 6-5 lines and of 13CO J = 3-2. The maps were analyzed by means of a heuristic 3D model that is able to reproduce the observations. In order to interpret this description of the molecule-rich nebula, we performed sophisticated calculations of hydrodynamical interaction and photoinduced chemistry. Results. We find that the CO-emitting gas is distributed within a relatively small region ≲ 1.″5. Its structure consists of a central dense component plus strongly disrupted outer regions, which seem to be parts of spiral arms that are highly focused on the orbital plane. The structure and dynamics of these spiral arms are compatible with our hydrodynamical calculations. We argue that the observed nebula is the result of the dynamical interaction between the wind and the gravitational attraction of the WD. We also find that UV emission from the hot companion efficiently photodissociates molecules except in the densest and best-shielded regions, that is, in the close surroundings of the AGB star and some shreds of the spiral arms from which the detected lines come. We can offer a faithful description of the distribution of nebular gas in this prototypical source, which will be a useful template for studying material around other tight binary systems.

Aims. We aim to constrain the sizes of, and investigate deviations from spherical symmetry in, the CO circumstellar envelopes (CSEs) of 16 S-type stars, along with an additional 7 and 4 CSEs of C-type and M-type AGB stars, respectively. Methods. We map the emission from the CO J = 2-1 and 3-2 lines observed with the Atacama Compact Array (ACA) and its total power (TP) antennas, and fit with a Gaussian distribution in the uv- and image planes for ACA-only and TP observations, respectively. The major axis of the fitted Gaussian for the CO(2-1) line data gives a first estimate of the size of the CO-line-emitting CSE. We investigate possible signs of deviation from spherical symmetry by analysing the line profiles and the minor-to-major axis ratio obtained from visibility fitting, and by investigating the deconvolved images. Results. The sizes of the CO-line-emitting CSEs of low-mass-loss-rate (low-MLR) S-type stars fall between the sizes of the CSEs of C-stars, which are larger, and those of M-stars, which are smaller, as expected because of the differences in their respective CO abundances and the dependence of the photodissociation rate on this quantity. The sizes of the low-MLR S-type stars show no dependence on circumstellar density, as measured by the ratio of the MLR to terminal outflow velocity, irrespective of variability type. The density dependence steepens for S-stars with higher MLRs. While the CO(2-1) brightness distribution size of the low-density S-stars is in general smaller than the predicted photodissociation radius (assuming the standard interstellar radiation field), the measured size of a few of the high-density sources is of the same order as the expected photodissociation radius. Furthermore, our results show that the CO CSEs of most of the S-stars in our sample are consistent with a spherically symmetric and smooth outflow. For some of the sources, clear and prominent asymmetric features are observed which are indicative of intrinsic circumstell

The Javalambre-Physics of the Accelerating Universe Astrophysical Survey (J-PAS) will soon start imaging thousands of square degrees of the northern sky with its unique set of 56 filters (spectral resolution of R - 60). Before the arrival of the final instrument, we observed 1 deg2 on the AEGIS field with an interim camera with all the J-PAS filters. Taking advantage of these data, dubbed miniJPAS, we aim at proving the scientific potential of the J-PAS to derive the stellar population properties of galaxies via fitting codes for spectral energy distributions (SEDs), with the ultimate goal of performing galaxy evolution studies across cosmic time. One parametric (BaySeAGal) and three non-parametric (MUFFIT, AlStar, and TGASPEX) SED-fitting codes are used to constrain the stellar mass, age, metallicity, extinction, and rest-frame and dust-corrected (u-r) colours of a complete flux-limited sample (rSDSS - 22.5 AB) of miniJPAS galaxies that extends up to z = 1. We generally find consistent results on the galaxy properties derived from the different codes, independently of the galaxy spectral type or redshift; this is remarkable considering that 25% of the J-spectra have signal-to-noise ratios (S/N) -3. For galaxies with S=N - 10, we estimate that the J-PAS photometric system will allow us to derive the stellar population properties of rest-frame (u - r) colour, stellar mass, extinction, and mass-weighted age with a precision of 0:04 - 0:02 mag, 0:07 - 0:03 dex, 0:2 - 0:09 mag, and 0:16 - 0:07 dex, respectively. This precision is equivalent to that obtained with spectroscopic surveys of similar S/N. By using the dust-corrected (u - r) colour mass diagram, a powerful proxy for characterizing galaxy populations, we find: (i) that the fraction of red and blue galaxies evolves with cosmic time, with red galaxies being -38% and -18% of the whole population at z = 0:1 and z = 0:5, respectively, and (ii) consistent results between codes for the average intrinsic (u-r) colour

Despite being a perishable material, wood can nonetheless show in its full complexity the materiality of daily life activities, identity construction, economic exploitation, and adaptation in colonial processes. The study of two sets of wood samples in well-defined archaeological colonial contexts from the site of Heping Dao, on the northern coast of Taiwan, has unveiled otherwise unknown aspects of native exchange, adoption of indigenous practices, and differences and similarities between early European colonialism and Japanese imperialism in Asia-Pacific. Despite the constraints of taxonomic identification in subtropical (and tropical) areas, the use of different coniferous wood types has been recorded: Cupressaceae, cf. Chamaecyparis spp., cf. Cryptomeria japonica and cf. Cunninghamia spp. The paper highlights the close relationship between wooden objects and diachronic historical processes and stresses the complexity of their study in colonial contexts, with implications toward the prehistoric period.

We present our current best estimate of the plausible observing scenarios for the Advanced LIGO, Advanced Virgo and KAGRA gravitational-wave detectors over the next several years, with the intention of providing information to facilitate planning for multi-messenger astronomy with gravitational waves. We estimate the sensitivity of the network to transient gravitational-wave signals for the third (O3), fourth (O4) and fifth observing (O5) runs, including the planned upgrades of the Advanced LIGO and Advanced Virgo detectors. We study the capability of the network to determine the sky location of the source for gravitational-wave signals from the inspiral of binary systems of compact objects, that is binary neutron star, neutron star–black hole, and binary black hole systems. The ability to localize the sources is given as a sky-area probability, luminosity distance, and comoving volume. The median sky localization area (90% credible region) is expected to be a few hundreds of square degrees for all types of binary systems during O3 with the Advanced LIGO and Virgo (HLV) network. The median sky localization area will improve to a few tens of square degrees during O4 with the Advanced LIGO, Virgo, and KAGRA (HLVK) network. During O3, the median localization volume (90% credible region) is expected to be on the order of 105,106,107Mpc3 for binary neutron star, neutron star–black hole, and binary black hole systems, respectively. The localization volume in O4 is expected to be about a factor two smaller than in O3. We predict a detection count of 1-1+12(10-10+52) for binary neutron star mergers, of 0-0+19(1-1+91) for neutron star–black hole mergers, and 17-11+22(79-44+89) for binary black hole mergers in a one-calendar-year observing run of the HLV network during O3 (HLVK network during O4). We evaluate sensitivity and localization expectations for unmodeled signal searches, including the search for intermediate mass black hole binary mergers.

We present 1.3 mm Atacama Large Millimeter/submillimeter Array observations of polarized dust emission toward the wide-binary protostellar system BHR 71 IRS1 and IRS2. IRS1 features what appears to be a natal, hourglass-shaped magnetic field. In contrast, IRS2 exhibits a magnetic field that has been affected by its bipolar outflow. Toward IRS2, the polarization is confined mainly to the outflow cavity walls. Along the northern edge of the redshifted outflow cavity of IRS2, the polarized emission is sandwiched between the outflow and a filament of cold, dense gas traced by ND, toward which no dust polarization is detected. This suggests that the origin of the enhanced polarization in IRS2 is the irradiation of the outflow cavity walls, which enables the alignment of dust grains with respect to the magnetic field - but only to a depth of ∼300 au, beyond which the dust is cold and unpolarized. However, in order to align grains deep enough in the cavity walls, and to produce the high polarization fraction seen in IRS2, the aligning photons are likely to be in the mid- to far-infrared range, which suggests a degree of grain growth beyond what is typically expected in very young, Class 0 sources. Finally, toward IRS1 we see a narrow, linear feature with a high (10%-20%) polarization fraction and a well-ordered magnetic field that is not associated with the bipolar outflow cavity. We speculate that this feature may be a magnetized accretion streamer; however, this has yet to be confirmed by kinematic observations of dense-gas tracers.

We present 1.3 mm ALMA dust polarization observations at a resolution of ∼0.02 pc for three massive molecular clumps, MM1, MM4, and MM9, in the infrared dark cloud G28.34+0.06. With these sensitive and high-resolution continuum data, MM1 is resolved into a cluster of condensations. The magnetic field structure in each clump is revealed by the polarized emission. We found a trend of decreasing polarized emission fraction with increasing Stokes I intensities in MM1 and MM4. Using the angular dispersion function method (a modified Davis-Chandrasekhar-Fermi method), the plane-of-sky magnetic field strengths in two massive dense cores, MM1-Core1 and MM4-Core4, are estimated to be ∼1.6 mG and ∼0.32 mG, respectively. The virial parameters in MM1-Core1 and MM4-Core4 are calculated to be ∼0.76 and ∼0.37, respectively, suggesting that massive star formation does not start in equilibrium. Using the polarization-intensity gradient-local gravity method, we found that the local gravity is closely aligned with intensity gradient in the three clumps, and the magnetic field tends to be aligned with the local gravity in MM1 and MM4 except for regions near the emission peak, which suggests that the gravity plays a dominant role in regulating the gas collapse. Half of the outflows in MM4 and MM9 are found to be aligned within 10 of the condensation-scale (<0.05 pc) magnetic field, indicating that the magnetic field could play an important role from condensation to disk scale in the early stage of massive star formation.

We present Atacama Large Millimeter/submillimeter Array (ALMA) and Karl G. Jansky Very Large Array (JVLA) observations of the massive infrared dark cloud NGC 6334S (also known as IRDC G350.56+0.44), located at the southwestern end of the NGC 6334 molecular cloud complex. The HCO and NHD lines covered by the ALMA observations at a ∼3″ angular resolution (∼0.02 pc) reveal that the spatially unresolved nonthermal motions are predominantly subsonic and transonic, a condition analogous to that found in low-mass star-forming molecular clouds. The observed supersonic nonthermal velocity dispersions in massive star-forming regions, often reported in the literature, might be significantly biased by poor spatial resolutions that broaden the observed line widths owing to unresolved motions within the telescope beam. Our 3 mm continuum image resolves 49 dense cores, whose masses range from 0.17 to 14 M . The majority of them are resolved with multiple velocity components. Our analyses of these gas velocity components find an anticorrelation between the gas mass and the virial parameter. This implies that the more massive structures tend to be more gravitationally unstable. Finally, we find that the external pressure in the NGC 6334S cloud is important in confining these dense structures and may play a role in the formation of dense cores and, subsequently, the embedded young stars.

[Aims] We aim to characterise certain physical properties of high-mass star-forming sites in the NGC 6334 molecular cloud, such as the core mass function (CMF), spatial distribution of cores, and mass segregation., [Methods] We used the Atacama Large Millimeter/sub-millimeter Array (ALMA) to image the embedded clusters NGC 6334-I and NGC 6334-I(N) in the continuum emission at 87.6 GHz. We achieved a spatial resolution of 1300 au, enough to resolve different compact cores and fragments, and to study the properties of the clusters., [Results] We detected 142 compact sources distributed over the whole surveyed area. The ALMA compact sources are clustered in different regions. We used different machine-learning algorithms to identify four main clusters: NGC 6334-I, NGC 6334-I(N), NGC 6334-I(NW), and NGC 6334-E. The typical separations between cluster members range from 4000 au to 12 000 au. These separations, together with the core masses (0.1–100 M⊙), are in agreement with the fragmentation being controlled by turbulence at scales of 0.1 pc. We find that the CMFs show an apparent excess of high-mass cores compared to the stellar initial mass function. We evaluated the effects of temperature and unresolved multiplicity on the derived slope of the CMF. Based on this, we conclude that the excess of high-mass cores might be spurious and due to inaccurate temperature determinations and/or resolution limitations. We searched for evidence of mass segregation in the clusters and we find that clusters NGC 6334-I and NGC 6334-I(N) show hints of segregation with the most massive cores located in the centre of the clusters., [Conclusions] We searched for correlations between the physical properties of the four embedded clusters and their evolutionary stage (based on the presence of H II regions and infrared sources). NGC 6334-E appears as the most evolved cluster, already harbouring a well-developed H II region. NGC 6334-I is the second-most evolved cluster with an ultra-compact H II region. NGC 6334-I(N) contains the largest population of dust cores distributed in two filamentary structures and no dominant H II region. Finally, NGC 6334-I(NW) is a cluster of mainly low-mass dust cores with no clear signs of massive cores or H II regions. We find a larger separation between cluster members in the more evolved clusters favouring the role of gas expulsion and stellar ejection with evolution. The mass segregation, seen in the NGC 6334-I and NGC 6334-I(N) clusters, suggests a primordial origin for NGC 6334-I(N). In contrast, the segregation in NGC 6334-I might be due to dynamical effects. Finally, the lack of massive cores in the most evolved cluster suggests that the gas reservoir is already exhausted, while the less evolved clusters still have a large gas reservoir along with the presence of massive cores. In general, the fragmentation process of NGC 6334 at large scales (from filament to clump, i.e. at about 1 pc) is likely governed by turbulent pressure, while at smaller scales (scale of cores and sub-fragments, i.e. a few hundred au) thermal pressure starts to be more significant.

A complex network of cellular receptors, RNA targeting pathways, and small-molecule signaling provides robust plant immunity and tolerance to viruses. To maximize their fitness, viruses must evolve control mechanisms to balance host immune evasion and plant-damaging effects. The genus Potyvirus comprises plant viruses characterized by RNA genomes that encode large polyproteins led by the P1 protease. A P1 autoinhibitory domain controls polyprotein processing, the release of a downstream functional RNA-silencing suppressor, and viral replication. Here, we show that P1Pro, a plum pox virus clone that lacks the P1 autoinhibitory domain, triggers complex reprogramming of the host transcriptome and high levels of abscisic acid (ABA) accumulation. A meta-analysis highlighted ABA connections with host pathways known to control RNA stability, turnover, maturation, and translation. Transcriptomic changes triggered by P1Pro infection or ABA showed similarities in host RNA abundance and diversity. Genetic and hormone treatment assays showed that ABA promotes plant resistance to potyviral infection. Finally, quantitative mathematical modeling of viral replication in the presence of defense pathways supported self-control of polyprotein processing kinetics as a viral mechanism that attenuates the magnitude of the host antiviral response. Overall, our findings indicate that ABA is an active player in plant antiviral immunity, which is nonetheless evaded by a self-controlled RNA virus.Molecular networks provide robust plant immunity against pathogens, including viruses. Here, meta-analyses indicate that abscisic acid (ABA) connects hormone signaling with RNA metabolic pathways and contributes to plant antiviral immunity, which is evaded by an RNA virus with self-controlled polyprotein-processing kinetics. This study reveals a regulatory mechanism that controls viral infection dynamics and the magnitude of host antiviral responses.

Context. This is the first publication from the DEATHSTAR project. The overall goal of the project is to reduce the uncertainties of the observational estimates of mass-loss rates from evolved stars on the Asymptotic Giant Branch (AGB). Aim. The aim in this first publication is to constrain the sizes of the 12CO emitting region from the circumstellar envelopes around 42 mostly southern AGB stars, of which 21 are M-Type and 21 are C-Type, using the Atacama Compact Array (ACA) at the Atacama Large Millimeter/submillimeter Array. The symmetry of the outflows is also investigated. Methods. Line emission from 12CO J = 2→1 and 3→2 from all of the sources were mapped using the ACA. In this initial analysis, the emission distribution was fit to a Gaussian distribution in the uv-plane. A detailed radiative transfer analysis will be presented in a future publication. The major and minor axis of the best-fit Gaussian at the line center velocity of the 12CO J = 2→1 emission gives a first indication of the size of the emitting region. Furthermore, the fitting results, such as the Gaussian major and minor axis, center position, and the goodness of fit across both lines, constrain the symmetry of the emission distribution. For a subsample of sources, the measured emission distribution is compared to predictions from previous best-fit radiative transfer modeling results. Results. We find that the CO envelope sizes are, in general, larger for C-Type than for M-Type AGB stars, which is as expected if the CO/H2 ratio is larger in C-Type stars. Furthermore, the measurements show a relation between the measured (Gaussian) 12CO J = 2→1 size and circumstellar density that, while in broad agreement with photodissociation calculations, reveals large scatter and some systematic differences between the different stellar types. For lower mass-loss-rate irregular and semi-regular variables of both M-and C-Type AGB stars, the 12CO J = 2→1 size appears to be independent of the ratio of the mass-l

Phosphorus (P) is an essential nutrient for plant growth and productivity. Due to soil fixation, however, phosphorus availability in soil is rarely sufficient to sustain high crop yields. The overuse of fertilizers to circumvent the limited bioavailability of phosphate (Pi) has led to a scenario of excessive soil P in agricultural soils. Whereas adaptive responses to Pi deficiency have been deeply studied, less is known about how plants adapt to Pi excess and how Pi excess might affect disease resistance. We show that high Pi fertilization, and subsequent Pi accumulation, enhances susceptibility to infection by the fungal pathogen Magnaporthe oryzae in rice. This fungus is the causal agent of the blast disease, one of the most damaging diseases of cultivated rice worldwide. Equally, MIR399f overexpression causes an increase in Pi content in rice leaves, which results in enhanced susceptibility to M. oryzae. During pathogen infection, a weaker activation of defence‐related genes occurs in rice plants over‐accumulating Pi in leaves, which is in agreement with the phenotype of blast susceptibility observed in these plants. These data support that Pi, when in excess, compromises defence mechanisms in rice while demonstrating that miR399 functions as a negative regulator of rice immunity. The two signalling pathways, Pi signalling and defence signalling, must operate in a coordinated manner in controlling disease resistance. This information provides a basis to understand the molecular mechanisms involved in immunity in rice plants under high Pi fertilization, an aspect that should be considered in management of the rice blast disease.

Aims. Our aim is to determine the distribution of stellar population parameters (extinction, age, metallicity, and star formation rates) of quiescent galaxies within the rest-frame stellar mass-colour diagrams and UVJ colour-colour diagrams corrected for extinction up to z similar to 1. These novel diagrams reduce the contamination in samples of quiescent galaxies owing to dust-reddened galaxies, and they provide useful constraints on stellar population parameters only using rest-frame colours and /or stellar mass. Methods. We set constraints on the stellar population parameters of quiescent galaxies combining the ALHAMBRA multi-filter photo-spectra with our fitting code for spectral energy distribution, MUlti-Filter FITting (MUFFIT), making use of composite stellar population models based on two independent sets of simple stellar population (SSP) models. The extinction obtained by MUFFIT allowed us to remove dusty star-forming (DSF) galaxies from the sample of red UVJ galaxies. The distributions of stellar population parameters across these rest-frame diagrams are revealed after the dust correction and are fitted by LOESS, a bi-dimensional and locally weighted regression method, to reduce uncertainty effects. Results. Quiescent galaxy samples defined via classical UVJ diagrams are typically contaminated by a similar to 20% fraction of DSF galaxies. A significant part of the galaxies in the green valley are actually obscured star-forming galaxies (similar to 30-65%). Consequently, the transition of galaxies from the blue cloud to the red sequence, and hence the related mechanisms for quenching, seems to be much more efficient and faster than previously reported. The rest-frame stellar mass-colour and UVJ colour-colour diagrams are useful for constraining the age, metallicity, extinction, and star formation rate of quiescent galaxies by only their redshift, rest-frame colours, and /or stellar mass. Dust correction plays an important role in understanding how quiescen

Aims. We aim at constraining the stellar population properties of quiescent galaxies. These properties reveal how these galaxies evolved and assembled since z similar to 1 up to the present time. Methods. Combining the ALHAMBRA multi-filter photo-spectra with the fitting code for spectral energy distribution MUFFIT (MUlti-Filter FITting), we built a complete catalogue of quiescent galaxies via the dust-corrected stellar mass vs. colour diagram. This catalogue includes stellar population properties, such as age, metallicity, extinction, stellar mass, and photometric redshift, retrieved from the analysis of composited populations based on two independent sets of simple stellar population (SSP) models. We developed and applied a novel methodology to provide, for the first time, the analytic probability distribution functions (PDFs) of mass-weighted age, metallicity, and extinction of quiescent galaxies as a function of redshift and stellar mass. We adopted different star formation histories to discard potential systematics in the analysis. Results. The number density of quiescent galaxies is found to increase since z similar to 1, with a more substantial variation at lower stellar mass. Quiescent galaxies feature extinction AV < 0.6, with median values in the range A(V) = 0.15-0.3. At increasing stellar mass, quiescent galaxies are older and more metal rich since z similar to 1. A detailed analysis of the PDFs reveals that the evolution of quiescent galaxies is not compatible with passive evolution and a slight decrease of 0.1-0.2 dex is hinted at median metallicity. The intrinsic dispersion of the age and metallicity PDFs show a dependence on stellar mass and/or redshift. These results are consistent with both sets of SSP models and assumptions of alternative star formation histories explored. Consequently, the quiescent population must undergo an evolutive pathway including mergers and/or remnants of star formation to reconcile the observed trends, where the >progeni

Aims. We perform a comprehensive study of the stellar population properties (formation epoch, age, metallicity, and extinction) of quiescent galaxies as a function of size and stellar mass to constrain the physical mechanism governing the stellar mass assembly and the likely evolutive scenarios that explain their growth in size. Methods. After selecting all the quiescent galaxies from the ALHAMBRA survey by the dust-corrected stellar mass-colour diagram, we built a shared sample of similar to 850 quiescent galaxies with reliable measurements of sizes from the HST. This sample is complete in stellar mass and luminosity, I <= 23. The stellar population properties were retrieved using the fitting code for spectral energy distributions called MUlti-Filter FITting for stellar population diagnostics (MUFFIT) with various sets of composite stellar population models. Age, formation epoch, metallicity, and extinction were studied on the stellar mass-size plane as function of size through a Monte Carlo approach. This accounted for uncertainties and degeneracy effects amongst stellar population properties. Results. The stellar population properties of quiescent galaxies and their stellar mass and size since z similar to 1 are correlated. At fixed stellar mass, the more compact the quiescent galaxy, the older and richer in metals it is (1 Gyr and 0.1 dex, respectively). In addition, more compact galaxies may present slight lower extinctions than their more extended counterparts at the same stellar mass (<0.1 mag). By means of studying constant regions of stellar population properties across the stellar mass-size plane, we obtained empirical relations to constrain the physical mechanism that governs the stellar mass assembly of the form M-star proportional to r(c)(alpha), where alpha amounts to 0.50-0.55 +/- 0.09. There are indications that support the idea that the velocity dispersion is tightly correlated with the stellar content of galaxies. The mechanisms driving the evoluti

[Background] MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in eukaryotes. In rice, MIR7695 expression is regulated by infection with the rice blast fungus Magnaporthe oryzae with subsequent down-regulation of an alternatively spliced transcript of natural resistance-associated macrophage protein 6 (OsNramp6). NRAMP6 functions as an iron transporter in rice., [Results] Rice plants grown under high iron supply showed blast resistance, which supports that iron is a factor in controlling blast resistance. During pathogen infection, iron accumulated in the vicinity of M. oryzae appressoria, the sites of pathogen entry, and in cells surrounding infected regions of the rice leaf. Activation-tagged MIR7695 rice plants (MIR7695-Ac) exhibited enhanced iron accumulation and resistance to M. oryzae infection. RNA-seq analysis revealed that blast resistance in MIR7695-Ac plants was associated with strong induction of defense-related genes, including pathogenesis-related and diterpenoid biosynthetic genes. Levels of phytoalexins during pathogen infection were higher in MIR7695-Ac than wild-type plants. Early phytoalexin biosynthetic genes, OsCPS2 and OsCPS4, were also highly upregulated in wild-type rice plants grown under high iron supply., [Conclusions] Our data support a positive role of miR7695 in regulating rice immunity that further underpin links between defense and iron signaling in rice. These findings provides a basis to better understand regulatory mechanisms involved in rice immunity in which miR7695 participates which has a great potential for the development of strategies to improve blast resistance in rice.

We have selected a sample of 11 massive clusters of galaxies observed by the Hubble Space Telescope in order to study the impact of the dynamical state on the intracluster light (ICL) fraction, the ratio of total integrated ICL to the total galaxy member light. With the exception of the Bullet cluster, the sample is drawn from the Cluster Lensing and Supernova Survey and the Frontier Fields program, containing five relaxed and six merging clusters. The ICL fraction is calculated in three optical filters using the CHEFs ICL estimator, a robust and accurate algorithm free of a priori assumptions. We find that the ICL fraction in the three bands is, on average, higher for the merging clusters, ranging between ∼7% and 23%, compared with the ∼2%-11% found for the relaxed systems. We observe a nearly constant value (within the error bars) in the ICL fraction of the regular clusters at the three wavelengths considered, which would indicate that the colors of the ICL and the cluster galaxies are, on average, coincident and, thus, so are their stellar populations. However, we find a higher ICL fraction in the F606W filter for the merging clusters, consistent with an excess of lower-metallicity/younger stars in the ICL, which could have migrated violently from the outskirts of the infalling galaxies during the merger event. © 2018. The American Astronomical Society. All rights reserved.

Almost half of known plant viral species rely on proteolytic cleavages as key co-and post-translational modifications throughout their infection cycle. Most of these viruses encode their own endopeptidases, proteases with high substrate specificity that internally cleave large polyprotein precursors for the release of functional subunits. Processing of the polyprotein, however, is not an all-or-nothing process in which endopeptidases act as simple peptide cutters. On the contrary, spatial-temporal modulation of these polyprotein cleavage events is crucial for a successful viral infection. In this way, the processing of the polyprotein coordinates viral replication, assembly and movement, and has significant impact on pathogen fitness and virulence. In this mini-review, we give an overview of plant viral proteases emphasizing their importance during viral infections and the varied functionalities that result from their proteolytic activities.

Context. The BL Lac object S5 0716+714, a highly variable blazar, underwent an impressive outburst in January 2015 (Phase A), followed by minor activity in February (Phase B). The MAGIC observations were triggered by the optical flux observed in Phase A, corresponding to the brightest ever reported state of the source in the R-band. Aims.The comprehensive dataset collected is investigated in order to shed light on the mechanism of the broadband emission. Methods. Multi-wavelength light curves have been studied together with the broadband spectral energy distributions (SEDs). The sample includes data from Effelsberg, OVRO, Metsähovi, VLBI, CARMA, IRAM, SMA, Swift-UVOT, KVA, Tuorla, Steward, RINGO3, KANATA, AZT-8+ST7, Perkins, LX-200, Swift-XRT, NuSTAR, Fermi-LAT and MAGIC. Results. The flaring state of Phase A was detected in all the energy bands, providing for the first time a multi-wavelength sample of simultaneous data from the radio band to the very-high-energy (VHE, E > 100 GeV). In the constructed SED, the Swift-XRT+NuSTAR data constrain the transition between the synchrotron and inverse Compton components very accurately, while the second peak is constrained from 0.1 GeV to 600 GeV by Fermi+MAGIC data. The broadband SED cannot be described with a one-zone synchrotron self-Compton model as it severely underestimates the optical flux in order to reproduce the X-ray to -ray data. Instead we use a two-zone model. The electric vector position angle (EVPA) shows an unprecedented fast rotation. An estimation of the redshift of the source by combined high-energy (HE, 0.1 GeV < E < 100 GeV) and VHE data provides a value of z = 0:31 ± 0:02 ± 0:05, confirming the literature value. Conclusions. The data show the VHE emission originating in the entrance and exit of a superluminal knot in and out of a recollimation shock in the inner jet. A shock-shock interaction in the jet seems responsible for the observed flares and EVPA swing. This scenario is also consistent with the

Type Ia supernovae arise from the thermonuclear explosion of white-dwarf stars that have cores of carbon and oxygen. The uniformity of their light curves makes these supernovae powerful cosmological distance indicators, but there have long been debates about exactly how their explosion is triggered and what kind of companion stars are involved. For example, the recent detection of the early ultraviolet pulse of a peculiar, subluminous type Ia supernova has been claimed as evidence for an interaction between a red-giant or a main-sequence companion and ejecta from a white-dwarf explosion. Here we report observations of a prominent but red optical flash that appears about half a day after the explosion of a type Ia supernova. This supernova shows hybrid features of different supernova subclasses, namely a light curve that is typical of normal-brightness supernovae, but with strong titanium absorption, which is commonly seen in the spectra of subluminous ones. We argue that this early flash does not occur through previously suggested mechanisms such as the companion¿ejecta interaction. Instead, our simulations show that it could occur through detonation of a thin helium shell either on a near-Chandrasekhar-mass white dwarf, or on a sub-Chandrasekhar-mass white dwarf merging with a less-massive white dwarf. Our finding provides evidence that one branch of previously proposed explosion models¿the helium-ignition branch¿does exist in nature, and that such a model may account for the explosions of white dwarfs in a mass range wider than previously supposed.

We present interferometric and single-dish molecular line observations of the interstellar bullet-outflow source IRAS 05506+2414, whose wide-angle bullet spray is similar to the Orion BN/KL explosive outflow and likely arises from an entirely different mechanism than the classical accretion-disk-driven bipolar flows in young stellar objects. The bullet-outflow source is associated with a large pseudo-disk and three molecular outflows - a high-velocity outflow (HVO), a medium-velocity outflow (MVO), and a slow, extended outflow (SEO). The size (mass) of the pseudo-disk is 10,350 au ×6400 au (0.64-0.17 M ); from a model-fit assuming infall and rotation, we derive a central stellar mass of 8-19 M . The HVO (MVO) has an angular size ∼5180 (∼3330) au and a projected outflow velocity of ∼140 km s (∼30 km s). The SEO size (outflow speed) is ∼0.9 pc (∼6 km s). The HVO's axis is aligned with (orthogonal to) that of the SEO (pseudo-disk). The velocity structure of the MVO is unresolved. The scalar momenta in the HVO and SEO are very similar, suggesting that the SEO has resulted from the HVO interacting with ambient-cloud material. The bullet spray shares a common axis with the pseudo-disk and has an age comparable to that of MVO (few hundred years), suggesting that these three structures are intimately linked. We discuss several models for the outflows in IRAS 05506+2414 (including dynamical decay of a stellar cluster, chance encounter of a runaway star with a dense cloud, and close passage of two protostars), and conclude that second-epoch imaging to derive proper motions of the bullets and nearby stars can help to discriminate between them.

Lignin is an essential polymer in vascular plants that plays key structural roles in vessels and fibers. Lignification is induced by external inputs such as wounding, but the molecular mechanisms that link this stress to lignification remain largely unknown. In this work, we provide evidence that three maize (Zea mays) lignin repressors, MYB11, MYB31, and MYB42, participate in wound-induced lignification by interacting with ZML2, a protein belonging to the TIFY family. We determined that the three R2R3-MYB factors and ZML2 bind in vivo to AC-rich and GAT(A/C) cis-elements, respectively, present in a set of lignin genes. In particular, we show that MYB11 and ZML2 bind simultaneously to the AC-rich and GAT(A/C) cis-elements present in the promoter of the caffeic acid O-methyl transferase (comt) gene. We show that, like the R2R3-MYB factors, ZML2 also acts as a transcriptional repressor. We found that upon wounding and methyl jasmonate treatments, MYB11 and ZML2 proteins are degraded and comt transcription is induced. Based on these results, we propose a molecular regulatory mechanism involving a MYB/ZML complex in which wound-induced lignification can be achieved by the derepression of a set of lignin genes.