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Abstract Myxozoa is a class of the Phylum Cnidaria made up of endoparasites from aquatic habitats. The genus Ceratomyxa preferentially infects marine fish, with the gallbladder being the main site parasitized. This study aimed to describe a new species of Ceratomyxa found in this organ in Boulengerella cuvieri using morphological, morphometric characterization and phylogenetic analysis of 18S rDNA gene sequences. Specimens of B. cuvieri were collected, anesthetized, desensitized and biometric measurements were performed. The organs were analyzed under a stereomicroscope and fragments of internal organs were extracted for light microscopy analysis, preserved in 80% ethanol for 18S rDNA gene analysis and fixed in Davidson solution for histological processing. Free spores of Ceratomyxa were observed in the gallbladder, in plasmodia with wave-like movements, with the following dimensions: spore width (24.5 ± 0.4) µm, spore length (5.2 ± 0.3) µm, polar capsule width (1.8 ± 0.2) µm, polar capsule length (2.1 ± 0.3) µm, number of polar tubule turns (4-5) and 100% prevalence. Phylogenetic analysis confirmed that Ceratomyxa matosi n. sp. is a new species, grouped with other freshwater Ceratomyxa species from the Amazon, representing the second description of species of this genus in the state of Amapá.

A travessia de pedestres durante o verde veicular é um problema que ainda necessita de maior compreensão e investigação, visto a complexidade das variáveis envolvidas e suas inter-relações. Ferramentas de coleta automatizada podem ser importantes aliadas na obtenção dessas variáveis e análise de suas inter-relações. O objetivo principal deste estudo é customizar e aplicar uma ferramenta automatizada para coletar variáveis importantes em estudos de travessias de pedestres em interseções semaforizadas, sendo estas os headways veiculares, os atrasos dos pedestres, as velocidades veiculares, os tipos de veículo e os instantes de travessia, por faixa. A ferramenta, aplicada em um vídeo de uma interseção semaforizada de Fortaleza, consistiu nas ferramentas YOLOv7 e StrongSORT. O mAP de treinamento da ferramenta foi de quase 90%. Ao todo, 9427 veículos e 723 pedestres foram rastreados; os headways mostraram grande amplitude, a velocidade média dos veículos foi de 28 km/h e o atraso médio dos pedestres foi de 18 seg. A validação com uma ferramenta de coleta (RUBA) apontou que não houve diferenças significativas nas coletas pelos dois métodos quanto aos instantes de passagem dos veículos e de seus headways; para as velocidades veiculares as diferenças foram entre ± 6 km/h, e para as variáveis dos pedestres, as médias das diferenças foram de até 0,2 seg.

ABSTRACT The objective of this case report was to share the successful management of severe endophthalmitis, aiming at ocular integrity and visual acuity. A 73-year-old man presented with visual acuity of 20/30 in the right eye and 20/200 in the left eye. On the 21st day postoperatively after phacoemulsification in the left eye, he developed symptoms of endophthalmitis, including ocular discomfort, blurred vision, and whitish discharge. Despite negative cultures, his condition worsened, resulting in corneal perforation on the 31st day. Conjunctival flap and penetrating keratoplasty were performed. Currently, the patient maintains a visual acuity of 20/40 in the left eye, with a healthy graft and no signs of failure. Despite the complications, careful follow-up and timely interventions successfully preserved his vision. The use of conjunctival flap during the inflammatory phase was crucial to maintaining ocular integrity. This underscores the importance of different approaches in complex ocular complications, including alternative strategies for ocular protection during active inflammation.

Land use modelling is essential to understanding future land use and change dynamics. Analyses of land use and change using Cellular Automata (CA) have been frequent in the literature since the latter were introduced to geography in the 1970s. In this paper, the statistical subsections – city blocks – of a previous analysis of Lisbon's commercial structure are used as the cells of a CA. A multi-layer perceptron (MLP) models the transition potential of those cells between 1995 and 2020, considering the impact of street centrality, subway development and other proxies of urban growth. The results confirm that commercial growth and change can be simulated as a function of street centrality and that using Local integration and Choice as drivers of a CA may help improve predictions of commercial growth and change. Moreover, the CA results reinforce that street centrality positively affects street commerce, especially when combined with access to transit and amenities. While these results can already inform planning practice and policymaking, the framework presented in this paper may be replicated to simulate ​future commercial land use, thus helping planners and policymakers prepare for change.

Although issues of equity and accessibility have already been addressed in transportation, especially with regard to the distribution of costs and benefits, there is no consensus on which concept and metric of fairness would be most appropriate for the evaluation of transportation infrastructure proposals. Normally, a utilitarian perspective is adopted, where issues of unequal distribution of costs and benefits are not the main focus. This paper aims to incorporate the assumptions of other justice theories, namely egalitarianism, communitarianism, and Capability Approach (CA), into the equity assessment of transportation infrastructures, and by doing so, pay closer attention to those who are less advantaged or more open to social exclusion. These theories are critically reviewed considering their contribution to the assessment of equity in terms of transportation infrastructure accessibility impacts. Based on the reviewed theories, accessibility indicators are built and used to assess the equity impacts of the Lisbon Metro expansion project. The findings support the importance of adding other justice perspectives to assessing transportation projects. The CA and Maximax support a need to establish minimum or acceptable distribution standards of accessibility. However, the results from the CA are strongly dependent on the assumptions as to the maximum acceptable travel times.

Background: Synovial hemangioma is a rare neoplastic lesion, which can result in recurrent hemarthrosis and pain. It can affect any joint, tendon, or bursa, withal those around the knee. The intra-articular lesion is more prevalent and is more frequently diagnosed in female children or young adults. Indications: Surgery is indicated when patients present with recurrent painful hemarthrosis that affect daily living and functionality. Technique Description: Diagnostic knee arthroscopy, initially without tourniquet inflation, was performed to detect and study the extent of the lesion. Afterwards, the tourniquet was inflated to proceed with the surgical excision. Medial parapatellar approach was performed, and limits of the hemangioma were identified. Wide resection was performed taking care to not damage the medial meniscus and medial condyle cartilage. Neoplastic lesion was sent to pathology analysis. The tourniquet was deflated and hemostasis checked because these lesions can present extensive bleeding. Results: Localized, well-circumscribed, and encapsulated lesions have been reported to usually present low recurrence rate when completely excised. Discussion/Conclusion: It is a rare disease, and around 200 cases have been reported; therefore, conclusions about treatment and outcomes rely mostly on case series and case reports. Early diagnosis and treatment are paramount to prevent degenerative changes secondary to recurrent hemarthrosis. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.

Graphical Abstract This is a visual representation of the abstract.

PurposeCystic fibrosis (CF) is a multisystem genetic disease caused by dysfunction of the epithelial anionic channel Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Decreased mucociliary clearance because of thickened mucus is part of the pulmonary disease pathophysiology. It is controversial if the thickened airway surface liquid (ASL) is caused by the deficient chloride secretion and excessive sodium (through ENaC) and water hyperabsorption from the periciliar fluid or by the lack of bicarbonate secretion with relative acidification of the ASL. Correlations between the magnitude of in vivo chloride conductance with phenotypic characteristics and CF genotype can help to elucidate these mechanisms and direct to new treatments.MethodsNasal potential difference was measured in 28 CF patients (age from 0.3 to 28 year) and correlated with pulmonary function, pancreatic phenotype, pulmonary colonization and genotype severity.ResultsThe CFTR-chloride conductance was better in older patients (r = 0.40; P = 0.03), in patients with better pulmonary function (r = 0.48; P = 0.01), and was associated with genotype severity. Higher chloride diffusion in the presence of a favorable chemical gradient was associated with Pseudomonas aeruginosa negativity (P

Objetivo: Esse estudo tem como objetivo mensurar o impacto da profilaxia antibiótica combinada nas taxas de Infecção de Sítio Cirúrgico (ISC) de pacientes submetidos à Redução Aberta de Fratura (RAF) e avaliar o impacto dessa medida no perfil de resistência antimicrobiana dos pacientes com diagnóstico de Infecção Relacionadas a Fraturas (IRF). Métodos: Estudo de coorte retrospectivo, unicêntrico, realizado em um hospital universitário, utilizando banco de dados do Serviço de Controle de Infecção Hospitalar (SCIH) de pacientes submetidos a RAF no período de janeiro a dezembro de 2022. Foram excluídos da análise pacientes com fratura exposta e menores de 18 anos. IRF foi definido de acordo com os critérios de ISC do Centers for Disease Control and Prevention. Paciente de elevado risco para ISC foi definido como índice de comorbidades de Charlson maior ou igual a cinco. Os pacientes foram seguidos por três meses do pós-operatório. De janeiro a março de 2022 foi utilizado cefalotina como profilaxia cirúrgica (grupo pré-intervenção) e de abril a dezembro o grupo intervenção utilizou cefuroxima + gentamicina para os pacientes de risco elevado ou cefuroxima em monoterapia para os demais. Resultados: No total, 1.901 pacientes foram incluídos no estudo, 864 pré-intervenção e 1.037 no grupo intervenção. As taxas de ISC do grupo pré vs. intervenção foram 6,9% (72) e 2,8% (24), respectivamente (RR=0,4; 95% IC 0,22‒0,58; p=0,000). No ano de 2022, os microrganismos mais frequentemente identificados na FRI foram S. aureus (28%), S. coagulase negativo (14%), Klebsiella aerogenes (13%) e Pseudomonas aeruginosa (9%). A profilaxia antibiótica estendida aplicada para pacientes com elevado risco de ISC não alterou a epidemiologia microbiana da IRF, enquanto não aumentou as taxas de microrganismos Multirresistentes (MDR) (p=0,784). Paralelamente, houve aumento na sensibilidade à oxacilina para Gram-positivos (pré-40% vs. 67%) e aumento na sensibilidade aos carbapenêmicos aos Gram-negativos (pré-82% e pós-100%). Conclusão: A profilaxia antibiótica estendida aplicada para um grupo selecionado de pacientes com maior risco de desenvolverem IRF não elevou as taxas de ISC por microrganismos multirresistentes, porém, contribuiu para redução significativa das taxas de ISC. Estratégias individualizadas de prevenção de ISC e mais estudos multicêntricos, especialmente para infecções associadas a biofilme são necessárias.

O volume temático Arqueologia e os Estudos do Lixo traz para a cena o que poderíamos chamar de Lixologia, aportuguesando o neologismo em inglês Garbology /Garbologia. O lixo quando definido como restos, coisas descartadas, abandonadas, geralmente com conteúdos que estão intimamente ligados ao cotidiano, é um vestígio precioso para a Arqueologia. Encontrar um bolsão de lixo em contexto estratigráfico preservado é um dos melhores achados que a pesquisa arqueológica pode oferecer para quem está escavando um sítio. Com a expansão de uma arqueologia mais preocupada com a genealogia de problemas socioambientais que assolam a vida em todo planeta, o interesse arqueológico pelo lixo tem ressurgido e com ele novos olhares para os clássicos de William Rathje e o uso da arqueologia em políticas públicas, assim como tem sido cada vez mais comuma referência a autores cujas diferentes perspectivas teórico-metodológicas unem-se no que vem sendo chamado de arqueologia do passado contemporâneo, do passado recente ou do presente.