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318 results on '"Abou-Gharbia, Magid"'

4. Effects of novel beta-lactam, MC-100093, and ceftriaxone on astrocytic glutamate transporters and neuroinflammatory factors in nucleus accumbens of C57BL/6 mice exposed to escalated doses of morphine

Author

Sari, Youssef, Swiss, Ghadeer M.S., Alrashedi, Fatin A., Baeshen, Kholoud A., Alshammari, Sultan A., Alsharari, Shakir D., Ali, Nemat, Alasmari, Abdullah F., Alhoshani, Ali, Alameen, Alaa A., Childers, Wayne E., Abou-Gharbia, Magid, and Alasmari, Fawaz

Published
2024
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7. Neuroinflammation and Neurometabolomic Profiling in Fentanyl Overdose Mouse Model Treated with Novel β-Lactam, MC-100093, and Ceftriaxone.

Author

Alasmari, Mohammed S., Alasmari, Fawaz, Alsharari, Shakir D., Alasmari, Abdullah F., Ali, Nemat, Ahamad, Syed Rizwan, Alghamdi, Abdullah M., Kadi, Aban A., Hammad, Alaa M., Ali, Yousif S. Mohamed, Childers, Wayne E., Abou-Gharbia, Magid, and Sari, Youssef

Subjects
GLUTAMATE transporters, NUCLEUS accumbens, INFLAMMATORY mediators, DRUG overdose, EXPOSURE dose, NALOXONE, FENTANYL
Abstract

Opioid-related deaths are attributed to overdoses, and fentanyl overdose has been on the rise in many parts of the world, including the USA. Glutamate transporter 1 (GLT-1) has been identified as a therapeutic target in several preclinical models of substance use disorders, and β-lactams effectively enhance its expression and function. In the current study, we characterized the metabolomic profile of the nucleus accumbens (NAc) in fentanyl-overdose mouse models, and we evaluated the protective effects of the functional enhancement of GLT-1 using β-lactams, ceftriaxone, and MC-100093. BALB/c mice were divided into four groups: control, fentanyl, fentanyl/ceftriaxone, and fentanyl/MC-100093. While the control group was intraperitoneally (i.p.) injected with normal saline simultaneously with other groups, all fentanyl groups were i.p. injected with 1 mg/kg of fentanyl as an overdose after habituation with four repetitive non-consecutive moderate doses (0.05 mg/kg) of fentanyl for a period of seven days. MC-100093 (50 mg/kg) and ceftriaxone (200 mg/kg) were i.p. injected from days 5 to 9. Gas chromatography–mass spectrometry (GC-MS) was used for metabolomics, and Western blotting was performed to determine the expression of target proteins. Y-maze spontaneous alternation performance and the open field activity monitoring system were used to measure behavioral manifestations. Fentanyl overdose altered the abundance of about 30 metabolites, reduced the expression of GLT-1, and induced the expression of inflammatory mediators IL-6 and TLR-4 in the NAc. MC-100093 and ceftriaxone attenuated the effects of fentanyl-induced downregulation of GLT-1 and upregulation of IL-6; however, only ceftriaxone attenuated fentanyl-induced upregulation of TRL4 expression. Both of the β-lactams attenuated the effects of fentanyl overdose on locomotor activities but did not induce significant changes in the overall metabolomic profile. Our findings revealed that the exposure to a high dose of fentanyl causes alterations in key metabolic pathways in the NAc. Pretreatment with ceftriaxone and MC-100093 normalized fentanyl-induced downregulation of GLT-1 expression with subsequent attenuation of neuroinflammation as well as the hyperactivity, indicating that β-lactams may be promising drugs for treating fentanyl use disorder. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Liver Metabolomics and Inflammatory Profiles in Mouse Model of Fentanyl Overdose Treated with Beta-Lactams

Author

Alasmari, Fawaz, primary, Alasmari, Mohammed S., additional, Assiri, Mohammed A., additional, Alswayyed, Mohammed, additional, Rizwan Ahamad, Syed, additional, Alhumaydhi, Abdulrahman I., additional, Arif, Bandar I., additional, Aljumayi, Sahar R., additional, AlAsmari, Abdullah F., additional, Ali, Nemat, additional, Childers, Wayne E., additional, Abou-Gharbia, Magid, additional, and Sari, Youssef, additional

Published
2023
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12. Table S1 from Targeting Calcium Signaling Induces Epigenetic Reactivation of Tumor Suppressor Genes in Cancer

Author

Raynal, Noël J.-M., primary, Lee, Justin T., primary, Wang, Youjun, primary, Beaudry, Annie, primary, Madireddi, Priyanka, primary, Garriga, Judith, primary, Malouf, Gabriel G., primary, Dumont, Sarah, primary, Dettman, Elisha J., primary, Gharibyan, Vazganush, primary, Ahmed, Saira, primary, Chung, Woonbok, primary, Childers, Wayne E., primary, Abou-Gharbia, Magid, primary, Henry, Ryan A., primary, Andrews, Andrew J., primary, Jelinek, Jaroslav, primary, Cui, Ying, primary, Baylin, Stephen B., primary, Gill, Donald L., primary, and Issa, Jean-Pierre J., primary

Published
2023
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13. Supplementary figures 1 through 14 from Targeting Calcium Signaling Induces Epigenetic Reactivation of Tumor Suppressor Genes in Cancer

Author

Raynal, Noël J.-M., primary, Lee, Justin T., primary, Wang, Youjun, primary, Beaudry, Annie, primary, Madireddi, Priyanka, primary, Garriga, Judith, primary, Malouf, Gabriel G., primary, Dumont, Sarah, primary, Dettman, Elisha J., primary, Gharibyan, Vazganush, primary, Ahmed, Saira, primary, Chung, Woonbok, primary, Childers, Wayne E., primary, Abou-Gharbia, Magid, primary, Henry, Ryan A., primary, Andrews, Andrew J., primary, Jelinek, Jaroslav, primary, Cui, Ying, primary, Baylin, Stephen B., primary, Gill, Donald L., primary, and Issa, Jean-Pierre J., primary

Published
2023
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14. Supplementary Materials and Methods and Supplementary Figure Legends from Targeting Calcium Signaling Induces Epigenetic Reactivation of Tumor Suppressor Genes in Cancer

Author

Raynal, Noël J.-M., primary, Lee, Justin T., primary, Wang, Youjun, primary, Beaudry, Annie, primary, Madireddi, Priyanka, primary, Garriga, Judith, primary, Malouf, Gabriel G., primary, Dumont, Sarah, primary, Dettman, Elisha J., primary, Gharibyan, Vazganush, primary, Ahmed, Saira, primary, Chung, Woonbok, primary, Childers, Wayne E., primary, Abou-Gharbia, Magid, primary, Henry, Ryan A., primary, Andrews, Andrew J., primary, Jelinek, Jaroslav, primary, Cui, Ying, primary, Baylin, Stephen B., primary, Gill, Donald L., primary, and Issa, Jean-Pierre J., primary

Published
2023
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15. Supplementary Table 2 from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway

Author

Crabtree, Judy S., primary, Jelinsky, Scott A., primary, Harris, Heather A., primary, Choe, Sung E., primary, Cotreau, Monette M., primary, Kimberland, Michelle L., primary, Wilson, Ewa, primary, Saraf, Kathryn A., primary, Liu, Wei, primary, McCampbell, Adrienne S., primary, Dave, Bhuvanesh, primary, Broaddus, Russell R., primary, Brown, Eugene L., primary, Kao, Wenling, primary, Skotnicki, Jerauld S., primary, Abou-Gharbia, Magid, primary, Winneker, Richard C., primary, and Walker, Cheryl L., primary

Published
2023
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16. Supplementary Methods from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway

Author

Crabtree, Judy S., primary, Jelinsky, Scott A., primary, Harris, Heather A., primary, Choe, Sung E., primary, Cotreau, Monette M., primary, Kimberland, Michelle L., primary, Wilson, Ewa, primary, Saraf, Kathryn A., primary, Liu, Wei, primary, McCampbell, Adrienne S., primary, Dave, Bhuvanesh, primary, Broaddus, Russell R., primary, Brown, Eugene L., primary, Kao, Wenling, primary, Skotnicki, Jerauld S., primary, Abou-Gharbia, Magid, primary, Winneker, Richard C., primary, and Walker, Cheryl L., primary

Published
2023
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17. Supplementary Table 1 from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway

Author

Crabtree, Judy S., primary, Jelinsky, Scott A., primary, Harris, Heather A., primary, Choe, Sung E., primary, Cotreau, Monette M., primary, Kimberland, Michelle L., primary, Wilson, Ewa, primary, Saraf, Kathryn A., primary, Liu, Wei, primary, McCampbell, Adrienne S., primary, Dave, Bhuvanesh, primary, Broaddus, Russell R., primary, Brown, Eugene L., primary, Kao, Wenling, primary, Skotnicki, Jerauld S., primary, Abou-Gharbia, Magid, primary, Winneker, Richard C., primary, and Walker, Cheryl L., primary

Published
2023
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18. Data from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway

Author

Crabtree, Judy S., primary, Jelinsky, Scott A., primary, Harris, Heather A., primary, Choe, Sung E., primary, Cotreau, Monette M., primary, Kimberland, Michelle L., primary, Wilson, Ewa, primary, Saraf, Kathryn A., primary, Liu, Wei, primary, McCampbell, Adrienne S., primary, Dave, Bhuvanesh, primary, Broaddus, Russell R., primary, Brown, Eugene L., primary, Kao, Wenling, primary, Skotnicki, Jerauld S., primary, Abou-Gharbia, Magid, primary, Winneker, Richard C., primary, and Walker, Cheryl L., primary

Published
2023
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19. Supplementary Table 4 from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway

Author

Crabtree, Judy S., primary, Jelinsky, Scott A., primary, Harris, Heather A., primary, Choe, Sung E., primary, Cotreau, Monette M., primary, Kimberland, Michelle L., primary, Wilson, Ewa, primary, Saraf, Kathryn A., primary, Liu, Wei, primary, McCampbell, Adrienne S., primary, Dave, Bhuvanesh, primary, Broaddus, Russell R., primary, Brown, Eugene L., primary, Kao, Wenling, primary, Skotnicki, Jerauld S., primary, Abou-Gharbia, Magid, primary, Winneker, Richard C., primary, and Walker, Cheryl L., primary

Published
2023
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20. Supplementary Table 3 from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway

Author

Crabtree, Judy S., primary, Jelinsky, Scott A., primary, Harris, Heather A., primary, Choe, Sung E., primary, Cotreau, Monette M., primary, Kimberland, Michelle L., primary, Wilson, Ewa, primary, Saraf, Kathryn A., primary, Liu, Wei, primary, McCampbell, Adrienne S., primary, Dave, Bhuvanesh, primary, Broaddus, Russell R., primary, Brown, Eugene L., primary, Kao, Wenling, primary, Skotnicki, Jerauld S., primary, Abou-Gharbia, Magid, primary, Winneker, Richard C., primary, and Walker, Cheryl L., primary

Published
2023
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21. Supplementary Figures 1-3 from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway

Author

Crabtree, Judy S., primary, Jelinsky, Scott A., primary, Harris, Heather A., primary, Choe, Sung E., primary, Cotreau, Monette M., primary, Kimberland, Michelle L., primary, Wilson, Ewa, primary, Saraf, Kathryn A., primary, Liu, Wei, primary, McCampbell, Adrienne S., primary, Dave, Bhuvanesh, primary, Broaddus, Russell R., primary, Brown, Eugene L., primary, Kao, Wenling, primary, Skotnicki, Jerauld S., primary, Abou-Gharbia, Magid, primary, Winneker, Richard C., primary, and Walker, Cheryl L., primary

Published
2023
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22. Supplementary Figure Legends 1-3 from Comparison of Human and Rat Uterine Leiomyomata: Identification of a Dysregulated Mammalian Target of Rapamycin Pathway

Author

Crabtree, Judy S., primary, Jelinsky, Scott A., primary, Harris, Heather A., primary, Choe, Sung E., primary, Cotreau, Monette M., primary, Kimberland, Michelle L., primary, Wilson, Ewa, primary, Saraf, Kathryn A., primary, Liu, Wei, primary, McCampbell, Adrienne S., primary, Dave, Bhuvanesh, primary, Broaddus, Russell R., primary, Brown, Eugene L., primary, Kao, Wenling, primary, Skotnicki, Jerauld S., primary, Abou-Gharbia, Magid, primary, Winneker, Richard C., primary, and Walker, Cheryl L., primary

Published
2023
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27. Enhanced Clearance of Aβ in Brain by Sustaining the Plasmin Proteolysis Cascade

Author

Jacobsen, J. Steven, Comery, Thomas A., Martone, Robert L., Elokdah, Hassan, Crandall, David L., Oganesian, Aram, Aschmies, Suzan, Kirksey, Yolanda, Gonzales, Cathleen, Xu, Jane, Zhou, Hua, Atchison, Kevin, Wagner, Erik, Zaleska, Margaret M., Das, Indranil, Arias, Robert L., Bard, Jonathan, Riddell, David, Gardell, Stephen J., Abou-Gharbia, Magid, Robichaud, Albert, Magolda, Ronald, Vlasuk, George P., Bjornsson, Thorir, Reinhart, Peter H., and Pangalos, Menelas N.

Published
2008
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28. Binding of Rapamycin Analogs to Calcium Channels and FKBP52 Contributes to Their Neuroprotective Activities

Author

Ruan, Benfang, Pong, Kevin, Jow, Flora, Bowlby, Mark, Crozier, Robert A., Liu, Danni, Liang, Shi, Chen, Yi, Mercado, Mary Lynn, Feng, Xidong, Bennett, Frann, von Schack, David, McDonald, Leonard, Zaleska, Margaret M., Wood, Andrew, Reinhart, Peter H., Magolda, Ronald L., Skotnicki, Jerauld, Pangalos, Menelas N., Koehn, Frank E., Carter, Guy T., Abou-Gharbia, Magid, and Graziani, Edmund I.

Published
2008
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31. Discovery of Novel Small-Molecule Inhibitors of SARS-CoV-2 Main Protease as Potential Leads for COVID-19 Treatment

Author

Manandhar, Anjela, primary, Srinivasulu, Vunnam, additional, Hamad, Mohamad, additional, Tarazi, Hamadeh, additional, Omar, Hany, additional, Colussi, Dennis J., additional, Gordon, John, additional, Childers, Wayne, additional, Klein, Michael L., additional, Al-Tel, Taleb H., additional, Abou-Gharbia, Magid, additional, and Elokely, Khaled M., additional

Published
2021
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32. Discovery of novel class of histone deacetylase inhibitors as potential anticancer agents

Author

El-Awady, Raafat, primary, Saleh, Ekram, additional, Hamoudi, Rifat, additional, Ramadan, Wafaa S., additional, Mazitschek, Ralph, additional, Nael, Manal A., additional, Elokely, Khaled M., additional, Abou-Gharbia, Magid, additional, Childers, Wayne E., additional, Srinivasulu, Vunnam, additional, Aloum, Lujain, additional, Menon, Varsha, additional, and Al-Tel, Taleb H., additional

Published
2021
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38. Discovery of Novel Class of Histone Deacetylase Inhibitors as Anticancer Agents

Author

El-Awady, Raafat, primary, Salah, Ekram, additional, Hamoudi, Rifat, additional, Mazitschek, Ralph, additional, Ramadan, Wafaa, additional, Nael, Manal, additional, Elokely, Khaled, additional, Abou-Gharbia, Magid, additional, Childers, Wayne, additional, Srinivas, Vunnam, additional, Aloum, Lujain, additional, Menon, Varsha, additional, and Al-Tel, Taleb, additional

Published
2021
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44. Design and synthesis of functionalized piperazin-1yl-(E)-stilbenes as inhibitors of 17α-hydroxylase-C17,20-lyase (Cyp17)

Author

Blass, Benjamin E., Iyer, Pravin, Abou-Gharbia, Magid, Childers, Wayne E., Gordon, John C., Ramanjulu, Mercy, Morton, George, Arumugam, Premkumar, Boruwa, Joshodeep, Ellingboe, John, Mitra, Sayan, Reddy Nimmareddy, Rajashekar, Paliwal, Shalini, Rajasekhar, Jamallamudi, Shivakumar, Savithiri, Srivastava, Pratima, Tangirala, Raghuram S., Venkataramanaiah, Konda, Bobbala, Ramreddy, Yanamandra, Mahesh, and Krishnakanth Reddy, L.

Published
2018
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48. Nuclear Magnetic Resonance Structure of the Human Polyoma JC Virus Agnoprotein

Author

Coric, Pascale, Saribas, A Sami, Abou-Gharbia, Magid, Childers, Wayne, Condra, Jon, White, Martyn, Safak, Mahmut, Bouaziz, Serge, Saribas, A. Sami, Laboratoire de cristallographie et RMN biologiques (LCRB - UMR 8015), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité de Pharmacologie Chimique et Génétique (UPCG - UMR_S 640/UMR 8151), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Bouaziz, Serge

Subjects
0301 basic medicine, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Stereochemistry, Viral protein, ALPHA-HELIX, JC virus, MESH: JC Virus, PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY, MESH: Protein Structure, Secondary, Peptide, medicine.disease_cause, Biochemistry, Oligomer, Protein Structure, Secondary, Article, 03 medical and health sciences, chemistry.chemical_compound, SV40, MESH: Nuclear Magnetic Resonance, Biomolecular, medicine, Humans, Viral Regulatory and Accessory Proteins, Nuclear Magnetic Resonance, Biomolecular, Molecular Biology, chemistry.chemical_classification, BKV, DIMER, MESH: Humans, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], INTRINSICALLY UNSTRUCTURED, POLYOMAVIRUS, OLIGOMER, Cell Biology, DNA, JC Virus, AGNOPROTEIN, MERKEL CELL, NMR, 3. Good health, Amino acid, JCV, 030104 developmental biology, chemistry, Viral replication, REPLICATION, Alpha helix, MESH: Viral Regulatory and Accessory Proteins
Abstract

International audience; Agnoprotein is an important regulatory protein of the human polyoma JC virus (JCV) and plays critical roles during the viral replication cycle. It forms highly stable dimers and oligomers through its Leu/Ile/Phe-rich domain, which is important for the stability and function of the protein. We recently resolved the partial 3D structure of this protein by NMR using a synthetic peptide encompassing amino acids Thr17 to Gln52, where the Leu/Ile/Phe- rich region was found to adopt a major alpha-helix conformation spanning amino acids 23-39. Here, we report the resolution of the 3D structure of full-length JCV agnoprotein by NMR, which not only confirmed the existence of the previously reported major α-helix domain at the same position but also revealed the presence of an additional minor α-helix region spanning amino acid residues Leu6 to lys13. The remaining regions of the protein adopt an intrinsically unstructured conformation. J. Cell. Biochem. 118: 3268-3280, 2017. © 2017 Wiley Periodicals, Inc.

Published
2017

49. Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer

Author

Zhang, Hanghang, primary, Pandey, Somnath, additional, Travers, Meghan, additional, Sun, Hongxing, additional, Morton, George, additional, Madzo, Jozef, additional, Chung, Woonbok, additional, Khowsathit, Jittasak, additional, Perez-Leal, Oscar, additional, Barrero, Carlos A., additional, Merali, Carmen, additional, Okamoto, Yasuyuki, additional, Sato, Takahiro, additional, Pan, Joshua, additional, Garriga, Judit, additional, Bhanu, Natarajan V., additional, Simithy, Johayra, additional, Patel, Bela, additional, Huang, Jian, additional, Raynal, Noël J.-M., additional, Garcia, Benjamin A., additional, Jacobson, Marlene A., additional, Kadoch, Cigall, additional, Merali, Salim, additional, Zhang, Yi, additional, Childers, Wayne, additional, Abou-Gharbia, Magid, additional, Karanicolas, John, additional, Baylin, Stephen B., additional, Zahnow, Cynthia A., additional, Jelinek, Jaroslav, additional, Graña, Xavier, additional, and Issa, Jean-Pierre J., additional

Published
2018
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