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Microalgae have the potential as a source of biofuels due to their high biomass productivity and ability to grow in a wide range of conditions, including wastewater. This study investigated cultivating two microalgae species, Oocystis pusilla and Chlorococcus infusionum, in wastewater for biodiesel production. Compared to Kühl medium, KC medium resulted in a significant fold increase in cellular dry weight production for both O. pusilla and C. infusionum, with an increase of 1.66 and 1.39, respectively. A concentration of 100% wastewater resulted in the highest growth for O. pusilla, with an increase in biomass and lipid content compared to the KC medium. C. infusionum could not survive in these conditions. For further increase in biomass and lipid yield of O. pusilla, different total dissolved solids (TDS) levels were used. Maximum biomass and lipid productivities were achieved at 3000 ppm TDS, resulting in a 28% increase in biomass (2.50 g/L) and a 158% increase in lipid yield (536.88 mg/g) compared to KC medium. The fatty acid profile of O. pusilla cultivated on aerated wastewater at 3000 ppm TDS showed a high proportion of desirable saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for biodiesel production. Cultivating microalgae in wastewater for biodiesel production can be cost-effective, especially for microalgae adapted to harsh conditions. It could be concluded that O. pusilla is a promising candidate for biodiesel production using wastewater as a growth medium, as it has high biomass productivity and lipid yield, and its fatty acid profile meets the standard values of American and European biodiesel standards. This approach offers a sustainable and environmentally friendly solution for producing biofuels while reducing the environmental impact of wastewater disposal., (© 2023. The Author(s).)

Currently, algae arouse a growing interest in the pharmaceutical and cosmetic area due to the fact that they have a great diversity of bioactive compounds with the potential for pharmacological and nutraceutical applications. Due to lifestyle modifications brought on by rapid urbanization, diabetes mellitus, a metabolic illness, is the third largest cause of death globally. The hunt for an efficient natural-based antidiabetic therapy is crucial to battling diabetes and the associated consequences due to the unfavorable side effects of currently available antidiabetic medications. Finding the possible advantages of algae for the control of diabetes is crucial for the creation of natural drugs. Many of algae's metabolic processes produce bioactive secondary metabolites, which give algae their diverse chemical and biological features. Numerous studies have demonstrated the antioxidant and antidiabetic benefits of algae, mostly by blocking carbohydrate hydrolyzing enzyme activity, such as α-amylase and α-glucosidase. Additionally, bioactive components from algae can lessen diabetic symptoms in vivo. Therefore, the current review concentrates on the role of various secondary bioactive substances found naturally in algae and their potential as antioxidants and antidiabetic materials, as well as the urgent need to apply these substances in the pharmaceutical industry.

Taking into consideration, the challenges faced by the environment and agro-ecosystem make increased for suggestions more reliable methods to help increase food security and deal with difficult environmental problems. Environmental factors play a critical role in the growth, development, and productivity of crop plants. Unfavorable changes in these factors, such as abiotic stresses, can result in plant growth deficiencies, yield reductions, long-lasting damage, and even death of the plants. In reflection of this, cyanobacteria are now considered important microorganisms that can improve the fertility of soils and the productivity of crop plants due to their different features like photosynthesis, great biomass yield, ability to fix the atmospheric N 2, capability to grow on non-arable lands, and varied water sources. Furthermore, numerous cyanobacteria consist of biologically active substances like pigments, amino acids, polysaccharides, phytohormones, and vitamins that support plant growth enhancement. Many studies have exposed the probable role of these compounds in the alleviation of abiotic stress in crop plants and have concluded with evidence of physiological, biochemical, and molecular mechanisms that confirm that cyanobacteria can decrease the stress and induce plant growth. This review discussed the promising effects of cyanobacteria and their possible mode of action to control the growth and development of crop plants as an effective method to overcome different stresses., Competing Interests: Competing InterestsThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© The Author(s) 2023.)

Background: Numerous pesticides and herbicides used in excess cause oxidative stress in plants. These chemicals protect plants from weeds and pests, but they also have very negative side effects, making them common abiotic stressors. One of the most significant nutritional crops in the world is the wheat plant. Conditions of herbicide stress have a negative impact on the plant's phonological phases and metabolic pathways. Plants primarily make an effort to adjust to the environment and develop oxidative homeostasis, which supports stress tolerance., Methods: When controlling broadleaf weeds that emerge after cereal crop plants have been planted, bromoxynil is frequently used as a selective-contact herbicide. This study looked at the effects of the cyanobacteria Arthrospira platensis and Nostoc muscorum aqueous extracts, tryptophan, and bromoxynil (Bh) alone or in combination on wheat plant growth parameters. Both tryptophan and cyanobacterial extract were used as chemical and natural safeners against Bh application. The antioxidant activity and transcriptome studies using qRT-PCR were assayed after 24, 48, 72, 96 h, and 15 days from Bh application in the vegetation stage of wheat plants (55 days old)., Results: In comparison with plants treated with Bh, wheat plants treated with cyanobacteria and tryptophan showed improvements in all growth parameters. Following application of Bh, wheat plants showed reduced glutathione content, as well as reduced antioxidant enzyme activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione-s-transferase. The combination of different treatments and Bh caused alleviation of the harmful effect induced by Bh on the measured parameters. Additionally, the expression of glutathione synthase and glutathione peroxidase, in addition to those of three genes ( Zeta , Tau , and Lambda ) of the GST gene family, was significantly upregulated when using Bh alone or in combination with different treatments, particularly after 24 h of treatment., Conclusion: The current study suggests using cyanobacterial extracts, particularly the A. platensis extract, for the development of an antioxidant defense system against herbicide toxicity, which would improve the metabolic response of developed wheat plants.

Upon Seeking natural and safe alternatives for synthetic medicines to treat many chronic diseases, seaweeds have offered a promising resource to produce numerous bioactive secondary metabolites. Through in vivo investigations, Turbinaria decurrens acetone extract (AE) revealed its antidiabetic activity against alloxan-induced diabetic rats. Treatment of rats with T. decurrens AE at 300 and 150 mg/Kg doses revealed antihyperglycemic activity by reducing the elevated blood glucose level. A remarkable decrease in the liver, kidney functions, and hyperlipidemia related to diabetes were also detected. Administration of the same extract also showed a recovery in body weight loss, total protein, albumin, and haemoglobin levels compared with untreated diabetic rats. Furthermore, treatment of rats with the same extract improved liver and pancreas histopathological disorders related to diabetes. These effects may be attributed to the presence of bioactive phytochemicals and antioxidant components in T. decurrens AE mainly cyclotrisiloxane, hexamethyl, and cyclic diterpene 3,7,11,15-tetramethyl-2-hexadecen-1-ol (phytol alcohol). Besides, other valuable secondary metabolites, as phenols, flavonoids, alkaloids, terpenoids, steroid and glycosides, which were documented and published by the same authors in a previous study. The obtained results in the present study recommended using T. decurrens AE in developing medicinal preparations for treatment of diabetes and its related symptoms.

Biogenic silver nanoparticles (bio-AgNPs) is one of the most fascinating nanomaterials used for several biomedical purposes. In the current study, we biosynthesized AgNPs (bio-AgNPs) using Arthrospira platensis (A-bio-AgNPs), Microcystis aeruginosa (M-bio-AgNPs), and Chlorella vulgaris (C-bio-AgNPs) active metabolites and evaluated their anticancer efficacy against breast cancer. The recovered bio-AgNPs were characterized using scanning and transmission electron microscopy (SEM and TEM). In addition, their safety profiles were monitored in vitro on PBMCs cells and in vivo on Albino mice. The obtained results indicated the safety usage of bio-AgNPs at concentrations of 0.1 mg/ml on PBMCs cells and 1.5 mg/ml on the Albino mice. The bio-AgNPs displayed dose-dependent cytotoxic effects against HepG-2, CaCO-2, and MCF-7 cell lines by inducing reactive oxygen species (ROS) and arresting the treated cells in G0/G1 and sub G0 phases. In addition, A-bio-AgNPs induced breast cancer cellular apoptosis by downregulating the expression of survivin, MMP7, TGF, and Bcl2 genes. Upon A-bio-AgNPs treatment, a significant reduction in tumor growth and prolonged survival rates were recorded in breast cancer BALB/c model. Furthermore, A-bio-AgNPs treatment significantly decreased the Ki-67 protein marker from 60% (in the untreated group) to 20% (in the treated group) and increased caspase-3 protein levels to 65% (in treated groups) comparing with 45% (in doxorubicin-treated groups)., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

The present study evaluated the sequential biodiesel-bioethanol production from seaweeds. A total of 22 macroalgal species were collected at different seasons and screened based on lipid and carbohydrate contents as well as biomass production. The promising species was selected, based on the relative increase in energy compounds (REEC, %), for further energy conversion. Seasonal and annual biomass yields of the studied species showed significant variations. The rhodophyte Amphiroa compressa and the chlorophyte Ulva intestinalis showed the highest annual biomass yield of 75.2 and 61.5 g m -2 year -1 , respectively. However, the highest annual carbohydrate productivity (ACP) and annual lipid productivity (ALP) were recorded for Ulva fasciata and Ulva intestinalis (17.0 and 3.0 g m -2 year -1 , respectively). The later was selected for further studies because it showed 14.8% higher REEC value than Ulva fasciata. Saturated fatty acids (SAFs) showed 73.4%, with palmitic acid as a dominant fatty acid (43.8%). Therefore, biodiesel showed high saturation degree, with average degree of unsaturation (ADU) of 0.508. All the measured biodiesel characteristics complied the international standards. The first route of biodiesel production (R1) from Ulva intestinalis showed biodiesel recovery of 32.3 mg g -1 dw. The hydrolysate obtained after saccharification of the whole biomass (R2) and lipid-free biomass (R3) contained 1.22 and 1.15 g L -1 , respectively, reducing sugars. However, bioethanol yield from R3 was 0.081 g g -1 dw, which represented 14.1% higher than that of R2. Therefore, application of sequential biofuel production using R3 resulted in gross energy output of 3.44 GJ ton -1 dw, which was 170.9% and 82.0% higher than R1 and R2, respectively. The present study recommended the naturally-grown Ulva intestinalis as a potential feedstock for enhanced energy recovery through sequential biodiesel-bioethanol production.

Generation of biodiesel from microalgae grown in wastewater can offer a cost-effective approach, whilst wastewaters usually do not contain the optimum concentrations of the essential nutrients and carbon sources that result in lowering the productivities of biomass and lipid. This study aimed to overcome this limitation by manipulating wastewater with various concentrations of nutrients (NO 3 - , PO 4 3- , Cl - and SO 4 2- ) and three carbon sources either individually or in combination to cultivate Micractinium reisseri for biodiesel production. Initially, various dilutions of wastewater were tested and a concentration up to 75% of wastewater showed the highest biomass productivity (0.076 g L -1 d -1 ) and lipid productivity (0.014 g L -1 d -1 ). The optimum manipulating conditions for maximum lipid production and the highest productivity required 50% decrease in phosphorous from the concentration of the control medium and supplementation with 1.0 g L -1 of glucose. Under this condition, biomass and lipid productivities increased by 1.7 and 4-folds, respectively, compared to those observed in the control. Furthermore, phosphorous starvation condition in the presence of glucose significantly improved fatty acid profile in the biomass and biodiesel quality related parameters., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The present work investigated the potential of the green alga Chlorella vulgaris to produce high-quality biofuel under culture stress conditions. The cultivation was carried out in a 1000 l open plate tank system, which provides biomass yields comparable to open pond systems, but with less area needed. Algal biomass and lipid content were measured repeatedly. We compared the two solvent systems n-hexane and hexane/isopropanol (HIP) for extraction efficiency of lipids and applied three different extraction methods Soxhlet, soaking, and soaking followed by Soxhlet (soak-Sox). The combination of the HIP solvent and the soak-Sox provided the highest lipid yield (15.8 ± 0.174). Volumetric biomass and lipid productivity were 0.201 g l -1  day -1 and 31.71 mg l -1  day -1 , respectively, whereas areal biomass and lipid productivity were 25.73 g m -2  day -1 and 4.066 g m -2  day -1 , respectively. The fatty acid profile by means of gas chromatography resulted in seven fatty acids from C 12 to C 18 . The most abundant fatty acid methyl esters (FAMES) were palmitic (C16:0), oleic (C18:1), and stearic (C18:0) acids. Lipid synthesis enhanced by optimizing the Kuhl growth medium with replacing nitrate by urea (50% N compared to the original recipe) increased salt content (10 g/l NaCl), ferrous sulfate (0.5 g/l), and sodium acetate addition (1 g/l). With regard to density, kinematic viscosity, gravity, pour point, flash point, and cetane number, the Chlorella-biodiesel comply with ASTM and EN standards thus pointing at the high potential of lipids synthesized by Chlorella as a feedstock for biodiesel production.

Lichen secondary metabolites (polyketides) are produced by the fungal partner, but the role of algal carbohydrates in polyketide biosynthesis is not clear. This study examined whether the type and concentration of algal carbohydrate explained differences in polyketide production and gene transcription by a lichen fungus (Cladonia rangiferina). The carbohydrates identified from a free-living cyanobacterium (Spirulina platensis; glucose), a lichen-forming alga (Diplosphaera chodatii; sorbitol) and the lichen alga that associates with C. rangiferina (Asterochloris sp.; ribitol) were used in each of 1%, 5% and 10% concentrations to enrich malt yeast extract media for culturing the mycobiont. Polyketides were determined by high performance liquid chromatography (HPLC), and polyketide synthase (PKS) gene transcription was measured by quantitative PCR of the ketosynthase domain of four PKS genes. The lower concentrations of carbohydrates induced the PKS gene expression where ribitol up-regulated CrPKS1 and CrPKS16 gene transcription and sorbitol up-regulated CrPKS3 and CrPKS7 gene transcription. The HPLC results revealed that lower concentrations of carbon sources increased polyketide production for three carbohydrates. One polyketide from the natural lichen thallus (fumarprotocetraric acid) also was produced by the fungal culture in ribitol supplemented media only. This study provides a better understanding of the role of the type and concentration of the carbon source in fungal polyketide biosynthesis in the lichen Cladonia rangiferina., (© 2016 by The Mycological Society of America.)

Enhancement of the immune response leading to protection against bacterial and fungal infections was shown using different schedules of immunization with microbial pigments and a polysaccharide. The group of mice given carotenoids of Rhodotorula glutinis (preparation I) and polysaccharide of Spitulina platensis (IV) survived for 2 weeks after Pseudomonas aeruginosa infection. The groups of mice given carotenoids (I), polysaccharide (IV), I+IV and with the crude phycocyanin of S. platensis (III)+IV survived for 2 weeks after Candida albicans infection. All other groups recorded a maximum level of mortality reaching 2 mice per group either after immunization or post-infection. Adding the carotenoids, phycocyanin and polysaccharides to food as additives might therefore enhance the human immune response against microbial infections.

The aim of the present study is to evaluate the biological control aptitude of the cyanobacteria, Anabaena subcylindrica, Nostoc muscorum and Oscillatoria angusta filtrates on the growth of the isolated pathogenic fungi from the different organs of Faba bean. Three caynobacterial (Anabaena subcylindrica, Nostoc muscorum and Oscillatoria angusta) filtrates were prepared in different concentrations and their effects on the isolated pathogenic fungi from leaves, stems and roots of Faba bean were studied. The study revealed high efficiency of the three algal filtrates on the control of the isolated pathogenic fungi from the three organs of Faba bean plants. The reduction in fungal mat growth diameter was greater than in that of the fungal dry weight showing inhibited fungal spread by greater rate. The reduction in the fungal dry weight was mostly linear and significantly correlated with the algal filtrate concentrations. The Efficient Algal Filtrate Concentration (EAFC) ranged between 104 and 461% for the three algal filtrates on the studied fungi dry weight. Complete control of the isolated fungi could be achieved by of a mixture of two algal filtrates in their EAFC and that of Nostoc muscorum + Oscillatoria angusta filtrates with an EAFC 368 and 194% were the best and economic mixture.

Protoplasts were obtained from the filamentous blue-green algaSpirulina platensis by treating the filaments with 0.05% (w/v) lysozyme in 0.03M phosphate buffer. The protoplasts regenerated cell walls and formed colonies when plated on a regeneration medium. The highest percentage of regeneration, 40% was obtained after 21 days.