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41 results on '"Abe, Katsumasa"'

15. Biochemical and genetic analysis of the [gamma]-resorcylate (2,6-dihydroxybenzoate) catabolic pathway in Rhizobium sp. strain MTP-10005: identification and functional analysis of its gene cluster

Author

Yoshida, Masahiro, Oikawa, Tadao, Obata, Hitoshi, Abe, Katsumasa, Mihara, Hisaaki, and Esaki, Nobuyoshi

Subjects
Rhizobium -- Genetic aspects, Rhizobium -- Research, Escherichia coli -- Genetic aspects, Escherichia coli -- Research, Genetic transcription -- Identification and classification, Genetic transcription -- Research, Biological sciences
Abstract

We identified a gene cluster that is involved in the [gamma]-resorcylate (2,6-dihydroxybenzoate) catabolism of the aerobic bacterium Rhizobium sp. strain MTP-10005. The cluster consists of the graRDAFCBEK genes, and graA, graB, graC, and graD were heterologously expressed in Escherichia coli. Enzymological studies showed that graD, graA, graC, and graB encode the reductase (GraD) and oxygenase (GraA) components of a resorcinol hydroxylase (EC 1.14.13.x), a maleylacetate reductase (GraC) (EC 1.3.1.32), and a hydroxyquinol 1,2-dioxygenase (GraB) (EC 1.13.11.37). Bioinformatic analyses suggested that graE, graR, and graK encode a protein with an unknown function (GraE), a MarR-type transcriptional regulator (GraR), and a benzoate transporter (GraK). Quantitative reverse transcription-PCR of graF, which encodes [gamma]-resorcylate decarboxylase, revealed that the maximum relative mRNA expression level ([5.93 [+ or -] 0.82] x [10.sup.-4]) of graF was detected in the total RNA of the cells after one hour of cultivation when [gamma]-resorcylate was used as the sole carbon source. Reverse transcription-PCR of graDAFCBE showed that these genes are transcribed as a single mRNA and that the transcription of the gene cluster is induced by [gamma]-resorcylate. These results suggested that the graDAFCBE genes are responsible as an operon for the growth of Rhizobium sp. strain MTP-10005 on [gamma]-resorcylate and are probably regulated by GraR at the transcriptional level. This is the first report of the [gamma]-resorcylate catabolic pathway in an aerobic bacterium.

Published
2007

22. Aspartate racemase and D-aspartate in starfish; possible involvement in testicular maturation

Author

Shibata, Kimihiko, Sugaya, Noriko, Kuboki, Yuko, Matsuda, Hiroko, Abe, Katsumasa, Shouji Takahashi, and Kera, Yoshio

Subjects
endocrine system, endocrine system diseases, urogenital system, nutritional and metabolic diseases, hormones, hormone substitutes, and hormone antagonists
Abstract

D-Aspartate, aspartate racemase activity, and D-aspartate oxidase activity were detected in tissues from several types of starfish. Aspartate racemase activity in male testes of Patiria pectinifera was significantly elevated in the summer months of the breeding season compared with spring months. We also compared aspartate racemase activity with the gonad index and found that activity in individuals with a gonad index ≥6% was four-fold higher than that of individuals with a gonad index

Published
2019
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26. Isolation and identification of persistent chlorinated organophosphorus flame retardant-degrading bacteria

Author

Takahashi, Shouji, Satake, Ikuko, Konuma, Isao, Kawashima, Koji, Kawasaki, Manami, Mori, Shingo, Morino, Jun, Mori, Junichi, Hongde Xu, Abe, Katsumasa, Yamada, Ryo-hei, and Kera, Yoshio

Subjects
Biodegradation -- Analysis, Bioremediation -- Analysis, Chlorination -- Analysis, Fire resistant materials -- Chemical properties, Microbial source tracking -- Research, Organophosphorus compounds -- Chemical properties, Biological sciences
Abstract

Two bacterial strains capable of degrading persistent chlorinated organophosphorus (OP) flame retardants, tris(2-chloroethyl) phosphate (TCEP) and tris(1,3-dichloro-2-propyl) phosphate (TDCPP), are isolated and identified. The strains are useful for the bioremediation of environments contaminated with these compounds.

Published
2010

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