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165 results on '"Abdel‐Aziem, Sekena H."'

45. Therapeutic and Prophylactic Efficacy of Garden Cress Seed Oil against Osteoporosis in Rats.

Author

makawy, Aida I.El, Mabrouk, Dalia M., Ibrahim, Faten M., Abdel-Aziem, Sekena H., and Sharaf, Hafiza A.

Subjects
TREATMENT effectiveness, OILSEEDS, OSTEOPOROSIS, MINERAL oils, BONES, TERIPARATIDE
Abstract

The target of this study was to estimate the efficacy of garden cress seed oil in the treatment of osteoporosis in rats. Seventy adult female Sprague-Dawley albino rats were used that were divided into seven groups (n-10/ group). Methotrexate (MTX) was subcutaneously injected (0.65 mg/kg) for two separate 5 days courses (5 days on/9 days off), to induce osteoporotic rat model. Garden cress seed oil was orally gavages with two patterns: the therapeutic pattern in which rats were orally gavages with oil in two doses (200 and 400 mg/ kg b w) after completion of injection of methotrexate. The prophylactic pattern rats were injected with methotrexate in concurrent with the oil at the same doses. The oils injection period for the two treated pattern was four weeks. After the completion of the treatment, animals were anesthetized and blood samples were collected for biochemical analysis. Femoral bones of the treated and control groups were collected to study the expression of genes associated with bone remodeling (Cathapsin K and TNF-αand Osterix). Bones histopathological and morphometric studies were performed. Garden cress oil improved minerals and vitamin D content in osteoporotic animals. The results of the gene expression showed that the oil reduced the gene expression level of Cathapsin K and TNF-α, while increased the level of Ostrix, leading to improving the histological structure and bone thickness of the osteoporotic animals. Garden cress seed oil considerably improved the osteoporosis bone marker through both therapeutic and prophylactic approaches. In addition, data revealed that the therapeutics pattern was more alleviative than prophylactic. [ABSTRACT FROM AUTHOR]

Published
2020

50. Assessment of Satureja montana L. and Mentha piperita L. Antioxidant Activity, Cytotoxicity and Pattern of Apoptotic Gene Expression in Hepatoma Cells.

Author

El makawy, Aida I., Ibrahim, Faten M., and Abdel-Aziem, Sekena H.

Subjects
PEPPERMINT, GENE expression, SAVORY (Herb), HEPATOCELLULAR carcinoma, ESSENTIAL oils, PHENOLS, PLANT mitochondria
Abstract

This study is intended to assess the Satureja montana L. and Mentha piperita L. var. citrata antiradical potential and elucidate their molecular mechanisms underlying the cytotoxic induction in HepG2 cells. The total phenolic and flavonoids of the selected plants were determined. Their antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazylhydrate (DPPH?), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS+) radicals and reducing power. The HepG2 cells were incubated with tested compounds at different concentrations ranging between 12.5 and 200µg/mL. Cytotoxicity was measured by MTT and the mRNA expression of Bcl-2, Bax and caspase-3 genes were estimated using Semi-quantitative RT-PCR. The results revealed that the S. montana and M. piperita aqueous extracts have high content of phenolic 188.2 ± 1.3 and 183.2 ± 0.9 mg GA/g and flavonoid 95.01±2.29 and 144.13±0.73 mg quercetin/g compounds. While their essential oils contain phenolic compounds 290.8 ± 1.4 and 9.2 ± 5.6 mg GA/g only. In vitro assays revealed a strong radical scavenging potential of the S. montana L. and M. piperita L. essential oils against stable DPPH?, ABTS+ and reducing power showing the highest radical quenching activity compared with the aqueous extracts. In addition, the extracts and oils of the two tested plants showed a concentration-dependent increase in growth inhibition against HepG2 cell line. Semi-quantitative RT-PCR established that either S. montana or M. piperita up-regulated HepG2 Bax and caspase-3 mRNA expression and down-regulated Bcl-2. Greater variation in Bax, Bcl-2 and caspase-3 expression were visible in the oils rather than the extracts. The decrease in Bcl-2, rise in Bax and lastly, the stimulation of caspase-3 in HepG2 cells indicated the irreversible apoptotic process. Accordingly, the results confirmed that selected plants have an antioxidant potential and apoptosis ability via the activation of mitochondrial apoptotic pathways. [ABSTRACT FROM AUTHOR]

Published
2019

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