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147 results on '"Abdallah, Abdallah M."'

4. Prevalence and risk factors of functional seizures among adult Sudanese patients with epilepsy, a cross-sectional clinic-based study

Author

Hussien Mohamed Ahmed, Khabab Abbasher, Elnaiem, Walaa, Abdalla, Yassin Abdelrahim, Hamza, Salih Boushra, Ibrahim, MuazA., Abdallah, Abdallah M., Hajhamed, Mawahib, Elfatih, Ghassan, Fakhreldeen, Aziza, Hassan, Tibyan, Faisal, Roaa, Salih, Rufaida A., Mahmoud, Mihad A., Ahmed, Mwaez, Fadlallah, Yousif, Alhusseini, Radi Tofaha, Albasheer, Nijood, Shamsaldeen, Lina, Mohammed, Leenah, Siddig, Amira, Alamin Alkhalifamohamed, Hussam Mohamedalhadi, Salih, Esraa Hassan, Hussien, Abbasher, and Fadelallah Eljack, Mohammed Mahmmoud

Published
2022
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5. CCN3, POSTN, and PTHLH as potential key regulators of genomic integrity and cellular survival in iPSCs

Author

Swaidan, Nuha T., primary, Soliman, Nada H., additional, Aboughalia, Ahmed T., additional, Darwish, Toqa, additional, Almeshal, Ruba O., additional, Al-Khulaifi, Azhar A., additional, Taha, Rowaida Z., additional, Alanany, Rania, additional, Hussein, Ahmed Y., additional, Salloum-Asfar, Salam, additional, Abdulla, Sara A., additional, Abdallah, Abdallah M., additional, and Emara, Mohamed M., additional

Published
2024
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6. Best Choice for Using the Nanoparticles Additives to Enhance the Growth of the Cultured Nile Tilapia Oreochromis niloticus.

Author

Abdallah, Abdallah M., Mehanna, Sahar F., Osman, Mohamed F., and Mahmoud, Sara M.

Subjects
*FISH feeds, *NILE tilapia, *FISH farming, *NANOPARTICLES, *FISHERIES, *FISH growth, *FEED additives
Abstract

Minerals deficiency in aquatic animals disturbs the biological manners and physiological functions. Wheras, nanoparticles play an important role in aquaculture, as they serve as essential minerals to increase fish growth and supplementation in feeds since many practical feedstuffs contain low levels of these microminerals. Dietary supplementation of nanoparticles produces greater survival, growth, antioxidant levels and immunity of aquatic organisms including fish. In this study, the diet of the Nile tilapia Oreochromis niloticus was supplemented with different levels of selenium nanoparticles (SeNPs), magnesium (MgNPs), and a mix of both. Moreover, the growth, survival and body vitality were evaluated after 50 days of feeding. During the experiment, the fish were fed diet of 30% crude protein twice a day. SeNPs, MgNPs, and a mix of both were added to the control diet at different levels of 0.0 (control). The diet consisted of different concentrations: 1 (T1 Se, T1 Mg & T1 mix), 3 (T2 Se, T2 Mg & T2 mix), 5 (T3 Se, T3 Mg & T3 mix), 6 (T4 Se, T4 Mg & T4 mix), and 7mg/kg (T5 Se, T5 Mg & T5 mix) diet to feed the Nile tilapia fingerlings whose average initial weight was 4.0± 1.0g. After a 2-week acclimation period, fish were randomly distributed into 16 glass tanks, each with a volume of 40 L, at a density of 25 fish per tank. The results indicate that the growth indices, feed efficiency and survival rate were significantly enhanced (P< 0.05) by incorporating a mix of Se-Mg NPs at a concentration of 6mg/kg diet. In conclusion, the supplementation of both SeNPs and Mg NPs together in the diet of O. niloticus showed a higher growth rate than those which fed by each NPs separately. These findings have beneficial implications for the performance of the fish farming industry. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Evolution and Strain Variation in BCG

Author

Abdallah, Abdallah M., Behr, Marcel A., COHEN, IRUN R., Series editor, LAJTHA, ABEL, Series editor, LAMBRIS, JOHN D., Series editor, PAOLETTI, RODOLFO, Series editor, REZAEI, NIMA, Series editor, and Gagneux, Sebastien, editor

Published
2017
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8. Outcomes, infectiousness, and transmission dynamics of patients with extensively drug-resistant tuberculosis and home-discharged patients with programmatically incurable tuberculosis: a prospective cohort study

Author

Dheda, Keertan, Limberis, Jason D, Pietersen, Elize, Phelan, Jody, Esmail, Aliasgar, Lesosky, Maia, Fennelly, Kevin P, te Riele, Julian, Mastrapa, Barbara, Streicher, Elizabeth M, Dolby, Tania, Abdallah, Abdallah M, Ben-Rached, Fathia, Simpson, John, Smith, Liezel, Gumbo, Tawanda, van Helden, Paul, Sirgel, Frederick A, McNerney, Ruth, Theron, Grant, Pain, Arnab, Clark, Taane G, and Warren, Robin M

Published
2017
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9. What is the right sequencing approach? Solo VS extended family analysis in consanguineous populations

Author

Alfares, Ahmed, Alsubaie, Lamia, Aloraini, Taghrid, Alaskar, Aljoharah, Althagafi, Azza, Alahmad, Ahmed, Rashid, Mamoon, Alswaid, Abdulrahman, Alothaim, Ali, Eyaid, Wafaa, Ababneh, Faroug, Albalwi, Mohammed, Alotaibi, Raniah, Almutairi, Mashael, Altharawi, Nouf, Alsamer, Alhanouf, Abdelhakim, Marwa, Kafkas, Senay, Mineta, Katsuhiko, Cheung, Nicole, Abdallah, Abdallah M., Büchmann-Møller, Stine, Fukasawa, Yoshinori, Zhao, Xiang, Rajan, Issaac, Hoehndorf, Robert, Al Mutairi, Fuad, Gojobori, Takashi, and Alfadhel, Majid

Published
2020
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11. Genome-wide analysis of multi- and extensively drug-resistant Mycobacterium tuberculosis

Author

Coll, Francesc, Phelan, Jody, Hill-Cawthorne, Grant A., Nair, Mridul B., Mallard, Kim, Ali, Shahjahan, Abdallah, Abdallah M., Alghamdi, Saad, Alsomali, Mona, Ahmed, Abdallah O., Portelli, Stephanie, Oppong, Yaa, Alves, Adriana, Bessa, Theolis Barbosa, Campino, Susana, Caws, Maxine, Chatterjee, Anirvan, Crampin, Amelia C., Dheda, Keertan, Furnham, Nicholas, Glynn, Judith R., Grandjean, Louis, Minh Ha, Dang, Hasan, Rumina, Hasan, Zahra, Hibberd, Martin L., Joloba, Moses, Jones-López, Edward C., Matsumoto, Tomoshige, Miranda, Anabela, Moore, David J., Mocillo, Nora, Panaiotov, Stefan, Parkhill, Julian, Penha, Carlos, Perdigão, João, Portugal, Isabel, Rchiad, Zineb, Robledo, Jaime, Sheen, Patricia, Shesha, Nashwa Talaat, Sirgel, Frik A., Sola, Christophe, Oliveira Sousa, Erivelton, Streicher, Elizabeth M., Helden, Paul Van, Viveiros, Miguel, Warren, Robert M., McNerney, Ruth, Pain, Arnab, and Clark, Taane G.

Published
2018
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16. Identification of Prognostic Metabolomic Biomarkers at the Interface of Mortality and Morbidity in Pre-Existing TB Cases Infected With SARS-CoV-2

Author

Diboun, I., Diboun, Ilhame, Cyprian, Farhan S., Anwardeen, Najeha Rizwana, Yassine, Hadi M., Elrayess, Mohamed A., Rahmoon, Samreen Mumtaz, Sayed, Sarah Khaled, Schuchardt, Sven, Khatib, Malkan, Bansal, Devendra, Farag, Elmoubashar Abu Baker Abd, Emara, Mohamed M., and Abdallah, Abdallah M.

Subjects
Microbiology (medical), Adult, Alanine, SARS-CoV-2, Immunology, COVID-19, biomarkers, Prognosis, Microbiology, metabolomics, Infectious Diseases, tuberculosis, Quality of Life, Humans, Tuberculosis, COVID-19 disease severity, Morbidity, post-tuberculosis long-term consequences
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection currently remains one of the biggest global challenges that can lead to acute respiratory distress syndrome (CARDS) in severe cases. In line with this, prior pulmonary tuberculosis (TB) is a risk factor for long-term respiratory impairment. Post-TB lung dysfunction often goes unrecognized, despite its relatively high prevalence and its association with reduced quality of life. In this study, we used a metabolomics analysis to identify potential biomarkers that aid in the prognosis of COVID-19 morbidity and mortality in post-TB infected patients. This analysis involved blood samples from 155 SARS-CoV-2 infected adults, of which 23 had a previous diagnosis of TB (post-TB), while 132 did not have a prior or current TB infection. Our analysis indicated that the vast majority (~92%) of post-TB individuals showed severe SARS-CoV-2 infection, required intensive oxygen support with a significantly high mortality rate (52.2%). Amongst individuals with severe COVID-19 symptoms, we report a significant decline in the levels of amino acids, notably the branched chains amino acids (BCAAs), more so in the post-TB cohort (FDR

Published
2022

17. Identification of Prognostic Metabolomic Biomarkers at the Interface of Mortality and Morbidity in Pre-Existing TB Cases Infected With SARS-CoV-2

Author

Diboun, Ilhame, primary, Cyprian, Farhan S., additional, Anwardeen, Najeha Rizwana, additional, Yassine, Hadi M., additional, Elrayess, Mohamed A., additional, Rahmoon, Samreen Mumtaz, additional, Sayed, Sarah Khaled, additional, Schuchardt, Sven, additional, Khatib, Malkan, additional, Bansal, Devendra, additional, Farag, Elmoubashar Abu Baker Abd, additional, Emara, Mohamed M., additional, and Abdallah, Abdallah M., additional

Published
2022
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18. Clinical characteristics, complications, and predictors of outcome of hospitalized adult Sudanese patients with COVID‐19 and malaria coinfection in Sudan: A multicenter retrospective cross‐sectional study

Author

Abbasher Hussien Mohamed Ahmed, Khabab, primary, Hasabo, Elfatih A., additional, Haroun, Mazin S., additional, Mah. Fadelallah Eljack, Moh., additional, Salih, Esraa Hassan, additional, Altayeb, Yousif F. O., additional, Nour, Alshareef B., additional, Abdallah, Abdallah M., additional, Osman, Waddah A. M., additional, and Yousif, Mohammed Y. E., additional

Published
2022
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21. Clinical Characteristics, Complications and Predictors of Outcome of Hospitalized Adult Sudanese Patients with COVID-19 and Malaria Co-Infection in Sudan, a Multi-Center Retrospective Cross-sectional Study

Author

Ahmed, Khabab Abbasher Hussien Mohamed, primary, Hasabo, Elfatih A., additional, Haroun, Mazin S., additional, Eljack, Moh.Mah.Fadelallah, additional, Altayeb, Yousif F. O., additional, Nour, Alshareef B., additional, Salih, Esraa Hassan, additional, Abdallah, Abdallah M., additional, Osman, Waddah Aljaely Mohammed, additional, and Yousif, Mohammed Yousif Elnaeem, additional

Published
2022
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27. Author Correction: Genome-wide analysis of multi- and extensively drug-resistant Mycobacterium tuberculosis

Author

Coll, Francesc, Phelan, Jody, Hill-Cawthorne, Grant A., Nair, Mridul B., Mallard, Kim, Ali, Shahjahan, Abdallah, Abdallah M., Alghamdi, Saad, Alsomali, Mona, Ahmed, Abdallah O., Portelli, Stephanie, Oppong, Yaa, Alves, Adriana, Bessa, Theolis Barbosa, Campino, Susana, Caws, Maxine, Chatterjee, Anirvan, Crampin, Amelia C., Dheda, Keertan, Furnham, Nicholas, Glynn, Judith R., Grandjean, Louis, Ha, Dang Minh, Hasan, Rumina, Hasan, Zahra, Hibberd, Martin L., Joloba, Moses, Jones-López, Edward C., Matsumoto, Tomoshige, Miranda, Anabela, Moore, David J., Mocillo, Nora, Panaiotov, Stefan, Parkhill, Julian, Penha, Carlos, Perdigão, João, Portugal, Isabel, Rchiad, Zineb, Robledo, Jaime, Sheen, Patricia, Shesha, Nashwa Talaat, Sirgel, Frik A., Sola, Christophe, Sousa, Erivelton Oliveira, Streicher, Elizabeth M., Van Helden, Paul, Viveiros, Miguel, Warren, Robert M., McNerney, Ruth, Pain, Arnab, and Clark, Taane G.

Published
2018
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28. Additional file 2 of What is the right sequencing approach? Solo VS extended family analysis in consanguineous populations

Author

Alfares, Ahmed, Alsubaie, Lamia, Aloraini, Taghrid, Aljoharah Alaskar, Azza Althagafi, Alahmad, Ahmed, Mamoon Rashid, Abdulrahman Alswaid, Alothaim, Ali, Eyaid, Wafaa, Faroug Ababneh, Albalwi, Mohammed, Raniah Alotaibi, Mashael Almutairi, Nouf Altharawi, Alhanouf Alsamer, Abdelhakim, Marwa, Senay Kafkas, Mineta, Katsuhiko, Cheung, Nicole, Abdallah, Abdallah M., Büchmann-Møller, Stine, Fukasawa, Yoshinori, Zhao, Xiang, Issaac Rajan, Hoehndorf, Robert, Mutairi, Fuad Al, Gojobori, Takashi, and Alfadhel, Majid

Subjects
Data_FILES
Abstract

Additional file 2.

Published
2020
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29. Mutagenesis-Based Characterization and Improvement of a Novel Inclusion Body Tag

Author

Jong, Wouter S. P., ten Hagen-Jongman, Corinne M., Vikström, David, Dontje, Wendy, Abdallah, Abdallah M., de Gier, Jan-Willem, Bitter, Wilbert, Luirink, Joen, Jong, Wouter S. P., ten Hagen-Jongman, Corinne M., Vikström, David, Dontje, Wendy, Abdallah, Abdallah M., de Gier, Jan-Willem, Bitter, Wilbert, and Luirink, Joen

Abstract

Whereas, bacterial inclusion bodies (IBs) for long were regarded as undesirable aggregates emerging during recombinant protein production, they currently receive attention as promising nanoparticulate biomaterials with diverse applications in biotechnology and biomedicine. We previously identified ssTorA, a signal sequence that normally directs protein export via the Tat pathway in E. coli, as a tag that induces the accumulation of fused proteins into IBs under overexpression conditions. Here, we used targeted mutagenesis to identify features and motifs being either critical or dispensable for IB formation. We found that IB formation is neither related to the function of ssTorA as a Tat-signal sequence nor is it a general feature of this family of signal sequences. IB formation was inhibited by co-overexpression of ssTorA binding chaperones TorD and DnaK and by amino acid substitutions that affect the propensity of ssTorA to form an alpha-helix. Systematic deletion experiments identified a minimal region of ssTorA required for IB formation in the center of the signal sequence. Unbiased genetic screening of a library of randomly mutagenized ssTorA sequences for reduced aggregation properties allowed us to pinpoint residues that are critical to sustain insoluble expression. Together, the data point to possible mechanisms for the aggregation of ssTorA fusions. Additionally, they led to the design of a tag with superior IB-formation properties compared to the original ssTorA sequence.

Published
2020
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34. Inhibition of autotransporter biogenesis by small molecules

Author

Steenhuis, Maurice, primary, Abdallah, Abdallah M., additional, de Munnik, Sabrina M., additional, Kuhne, Sebastiaan, additional, Sterk, Geert‐Jan, additional, van den Berg van Saparoea, Bart, additional, Westerhausen, Sibel, additional, Wagner, Samuel, additional, van der Wel, Nicole N., additional, Wijtmans, Maikel, additional, van Ulsen, Peter, additional, Jong, Wouter S. P., additional, and Luirink, Joen, additional

Published
2019
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35. Integrated transcriptomic and proteomic analysis of pathogenic mycobacteria and their esx-1 mutants reveal secretion-dependent regulation of ESX-1 substrates and WhiB6 as a transcriptional regulator

Author

Abdallah, Abdallah M., primary, Weerdenburg, Eveline M., additional, Guan, Qingtian, additional, Ummels, Roy, additional, Borggreve, Stephanie, additional, Adroub, Sabir A., additional, Malas, Tareq B., additional, Naeem, Raeece, additional, Zhang, Huoming, additional, Otto, Thomas D., additional, Bitter, Wilbert, additional, and Pain, Arnab, additional

Published
2019
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36. Accelerating early antituberculosis drug discovery by creating mycobacterial indicator strains that predict mode of action

Author

Boot, Maikel, Commandeur, Susanna, Subudhi, Amit K., Bahira, Meriem, Smith, Trever C., Abdallah, Abdallah M., van Gemert, Mae, Lelièvre, Joël, Ballell, Lluís, Aldridge, Bree B., Pain, Arnab, Speer, Alexander, Bitter, Wilbert, Boot, Maikel, Commandeur, Susanna, Subudhi, Amit K., Bahira, Meriem, Smith, Trever C., Abdallah, Abdallah M., van Gemert, Mae, Lelièvre, Joël, Ballell, Lluís, Aldridge, Bree B., Pain, Arnab, Speer, Alexander, and Bitter, Wilbert

Abstract

Due to the rise of drug-resistant forms of tuberculosis, there is an urgent need for novel antibiotics to effectively combat these cases and shorten treatment regimens. Recently, drug screens using whole-cell analyses have been shown to be successful. However, current high-throughput screens focus mostly on stricto sensu life/death screening that give little qualitative information. In doing so, promising compound scaffolds or nonoptimized compounds that fail to reach inhibitory concentrations are missed. To accelerate early tuberculosis (TB) drug discovery, we performed RNA sequencing on Mycobacterium tuberculosis and Mycobacterium marinum to map the stress responses that follow upon exposure to subinhibitory concentrations of antibiotics with known targets, ciprofloxacin, ethambutol, isoniazid, streptomycin, and rifampin. The resulting data set comprises the first overview of transcriptional stress responses of mycobacteria to different antibiotics. We show that antibiotics can be distinguished based on their specific transcriptional stress fingerprint. Notably, this fingerprint was more distinctive in M. marinum. We decided to use this to our advantage and continue with this model organism. A selection of diverse antibiotic stress genes was used to construct stress reporters. In total, three functional reporters were constructed to respond to DNA damage, cell wall damage, and ribosomal inhibition. Subsequently, these reporter strains were used to screen a small anti-TB compound library to predict the mode of action. In doing so, we identified the putative modes of action for three novel compounds, which confirms the utility of our approach.

Published
2018
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37. Genome-wide analysis of multi- and extensively drug-resistant Mycobacterium tuberculosis

Author

King Abdullah University of Science and Technology, Wellcome Trust, Burroughs Wellcome Fund, Fundação para a Ciência e a Tecnologia (Portugal), Fundação Calouste Gulbenkian, European Society of Clinical Microbiology and Infectious Diseases, Medical Research Council (UK), Biotechnology and Biological Sciences Research Council (UK), Japan Agency for Medical Research and Development, Coll, Francesc [0000-0002-7882-2325], Coll, Francesc, Phelan, Jody, Hill-Cawthorne, Grant A., Nair, Mridul B., Mallard, Kim, Ali, Shahjahan, Abdallah, Abdallah M., Alghamdi, Saad, Alsomali, Mona, Ahmed, Abdallah O., Portelli, Stephanie, Oppong, Yaa, Alves, Adriana, Barbosa Bessa, Theolis, Campino, Susana, Caws, Maxine, Chatterjee, Anirvan, Crampin, Amelia C., Dheda, Keertan, Furnham, Nicholas, Glynn, Judith R., Grandjean, Louis Louis, Ha, Dang Minh, Hasan, Rumina, Hasan, Zahra, Hibberd, Martin L., Joloba, Moses, Jones-López, Edward C., Matsumoto, Tomoshige, Miranda, Anabela, Moore, David J., Mocillo, Nora, Panaiotov, Stefan, Parkhill, Julian, Penha, Carlos, Perdigão, João, Portugal, Isabel, Rchiad, Zineb, Robledo, Jaime, Sheen, Patricia, Shesha, Nashwa Talaat, Sirgel, Frik A., Sola, Christophe, Oliveira Sousa, Erivelton, Streicher, Elizabeth M., Helden, Paul Van, Viveiros, Miguel, Warren, Robert M., McNerney, Ruth, Pain, Arnab, Clark, Taane G., King Abdullah University of Science and Technology, Wellcome Trust, Burroughs Wellcome Fund, Fundação para a Ciência e a Tecnologia (Portugal), Fundação Calouste Gulbenkian, European Society of Clinical Microbiology and Infectious Diseases, Medical Research Council (UK), Biotechnology and Biological Sciences Research Council (UK), Japan Agency for Medical Research and Development, Coll, Francesc [0000-0002-7882-2325], Coll, Francesc, Phelan, Jody, Hill-Cawthorne, Grant A., Nair, Mridul B., Mallard, Kim, Ali, Shahjahan, Abdallah, Abdallah M., Alghamdi, Saad, Alsomali, Mona, Ahmed, Abdallah O., Portelli, Stephanie, Oppong, Yaa, Alves, Adriana, Barbosa Bessa, Theolis, Campino, Susana, Caws, Maxine, Chatterjee, Anirvan, Crampin, Amelia C., Dheda, Keertan, Furnham, Nicholas, Glynn, Judith R., Grandjean, Louis Louis, Ha, Dang Minh, Hasan, Rumina, Hasan, Zahra, Hibberd, Martin L., Joloba, Moses, Jones-López, Edward C., Matsumoto, Tomoshige, Miranda, Anabela, Moore, David J., Mocillo, Nora, Panaiotov, Stefan, Parkhill, Julian, Penha, Carlos, Perdigão, João, Portugal, Isabel, Rchiad, Zineb, Robledo, Jaime, Sheen, Patricia, Shesha, Nashwa Talaat, Sirgel, Frik A., Sola, Christophe, Oliveira Sousa, Erivelton, Streicher, Elizabeth M., Helden, Paul Van, Viveiros, Miguel, Warren, Robert M., McNerney, Ruth, Pain, Arnab, and Clark, Taane G.

Abstract

To characterize the genetic determinants of resistance to antituberculosis drugs, we performed a genome-wide association study (GWAS) of 6,465 Mycobacterium tuberculosis clinical isolates from more than 30 countries. A GWAS approach within a mixed-regression framework was followed by a phylogenetics-based test for independent mutations. In addition to mutations in established and recently described resistance-associated genes, novel mutations were discovered for resistance to cycloserine, ethionamide and para-aminosalicylic acid. The capacity to detect mutations associated with resistance to ethionamide, pyrazinamide, capreomycin, cycloserine and para-aminosalicylic acid was enhanced by inclusion of insertions and deletions. Odds ratios for mutations within candidate genes were found to reflect levels of resistance. New epistatic relationships between candidate drug-resistance-associated genes were identified. Findings also suggest the involvement of efflux pumps (drrA and Rv2688c) in the emergence of resistance. This study will inform the design of new diagnostic tests and expedite the investigation of resistance and compensatory epistatic mechanisms.

Published
2018

38. Accelerating Early Antituberculosis Drug Discovery by Creating Mycobacterial Indicator Strains That Predict Mode of Action

Author

Boot, Maikel, primary, Commandeur, Susanna, additional, Subudhi, Amit K., additional, Bahira, Meriem, additional, Smith, Trever C., additional, Abdallah, Abdallah M., additional, van Gemert, Mae, additional, Lelièvre, Joël, additional, Ballell, Lluís, additional, Aldridge, Bree B., additional, Pain, Arnab, additional, Speer, Alexander, additional, and Bitter, Wilbert, additional

Published
2018
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40. ESX-5-deficient Mycobacterium marinum is hypervirulent in adult zebrafish

Author

Weerdenburg, Eveline M., Abdallah, Abdallah M., Mitra, Suman, de Punder, Karin, van der Wel, Nicole N., Bird, Steve, Appelmelk, Ben J., Bitter, Wilbert, van der Sar, Astrid M., and Academic Medical Center

Subjects
animal structures
Abstract

ESX-5 is a mycobacterial type VII protein secretion system responsible for transport of numerous PE and PPE proteins. It is involved in the induction of host cell death and modulation of the cytokine response in vitro. In this work, we studied the effects of ESX-5 in embryonic and adult zebrafish using Mycobacterium marinum. We found that ESX-5-deficient M. marinum was slightly attenuated in zebrafish embryos. Surprisingly, the same mutant showed highly increased virulence in adult zebrafish, characterized by increased bacterial loads and early onset of granuloma formation with rapid development of necrotic centres. This early onset of granuloma formation was accompanied by an increased expression of pro-inflammatory cytokines and tissue remodelling genes in zebrafish infected with the ESX-5 mutant. Experiments using RAG-1-deficient zebrafish showed that the increased virulence of the ESX-5 mutant was not dependent on the adaptive immune system. Mixed infection experiments with wild-type and ESX-5 mutant bacteria showed that the latter had a specific advantage in adult zebrafish and outcompeted wild-type bacteria. Together our experiments indicate that ESX-5-mediated protein secretion is used by M. marinum to establish a moderate and persistent infection

Published
2012
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42. Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations

Author

Abdallah, Abdallah M., primary, Hill-Cawthorne, Grant A., additional, Otto, Thomas D., additional, Coll, Francesc, additional, Guerra-Assunção, José Afonso, additional, Gao, Ge, additional, Naeem, Raeece, additional, Ansari, Hifzur, additional, Malas, Tareq B., additional, Adroub, Sabir A., additional, Verboom, Theo, additional, Ummels, Roy, additional, Zhang, Huoming, additional, Panigrahi, Aswini Kumar, additional, McNerney, Ruth, additional, Brosch, Roland, additional, Clark, Taane G., additional, Behr, Marcel A., additional, Bitter, Wilbert, additional, and Pain, Arnab, additional

Published
2015
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43. Essential Role of the ESX-5 Secretion System in Outer Membrane Permeability of Pathogenic Mycobacteria

Author

Ates, Louis S., primary, Ummels, Roy, additional, Commandeur, Susanna, additional, van der Weerd, Robert, additional, Sparrius, Marion, additional, Weerdenburg, Eveline, additional, Alber, Marina, additional, Kalscheuer, Rainer, additional, Piersma, Sander R., additional, Abdallah, Abdallah M., additional, Abd El Ghany, Moataz, additional, Abdel-Haleem, Alyaa M., additional, Pain, Arnab, additional, Jiménez, Connie R., additional, Bitter, Wilbert, additional, and Houben, Edith N.G., additional

Published
2015
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44. Genome-Wide Transposon Mutagenesis Indicates that Mycobacterium marinum Customizes Its Virulence Mechanisms for Survival and Replication in Different Hosts

Author

Weerdenburg, Eveline M., primary, Abdallah, Abdallah M., additional, Rangkuti, Farania, additional, Abd El Ghany, Moataz, additional, Otto, Thomas D., additional, Adroub, Sabir A., additional, Molenaar, Douwe, additional, Ummels, Roy, additional, ter Veen, Kars, additional, van Stempvoort, Gunny, additional, van der Sar, Astrid M., additional, Ali, Shahjahan, additional, Langridge, Gemma C., additional, Thomson, Nicholas R., additional, Pain, Arnab, additional, and Bitter, Wilbert, additional

Published
2015
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47. Complete Genome Sequence of Mycobacterium vaccae Type Strain ATCC 25954

Author

Ho, Yung S., primary, Adroub, Sabir A., additional, Abadi, Maram, additional, Al Alwan, Bader, additional, Alkhateeb, Reham, additional, Gao, Ge, additional, Ragab, Alaa, additional, Ali, Shahjahan, additional, van Soolingen, Dick, additional, Bitter, Wilbert, additional, Pain, Arnab, additional, and Abdallah, Abdallah M., additional

Published
2012
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50. ESX‐1‐mediated translocation to the cytosol controls virulence of mycobacteria

Author

Houben, Diane, primary, Demangel, Caroline, additional, van Ingen, Jakko, additional, Perez, Jorge, additional, Baldeón, Lucy, additional, Abdallah, Abdallah M., additional, Caleechurn, Laxmee, additional, Bottai, Daria, additional, van Zon, Maaike, additional, de Punder, Karin, additional, van der Laan, Tridia, additional, Kant, Arie, additional, Bossers‐de Vries, Ruth, additional, Willemsen, Peter, additional, Bitter, Wilbert, additional, van Soolingen, Dick, additional, Brosch, Roland, additional, van der Wel, Nicole, additional, and Peters, Peter J., additional

Published
2012
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