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35 results on '"Abbondio, Marcello"'

9. Metagenomic Changes of Gut Microbiota following Treatment of Helicobacter pylori Infection with a Simplified Low-Dose Quadruple Therapy with Bismuth or Lactobacillus reuteri

Author

Dore, Maria Pina, primary, Sau, Rosangela, additional, Niolu, Caterina, additional, Abbondio, Marcello, additional, Tanca, Alessandro, additional, Bibbò, Stefano, additional, Loria, Mariafrancesca, additional, Pes, Giovanni Mario, additional, and Uzzau, Sergio, additional

Published
2022
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10. Metaproteomic Profile of the Colonic Luminal Microbiota From Patients With Colon Cancer

Author

Tanca, Alessandro, primary, Abbondio, Marcello, additional, Fiorito, Giovanni, additional, Pira, Giovanna, additional, Sau, Rosangela, additional, Manca, Alessandra, additional, Muroni, Maria Rosaria, additional, Porcu, Alberto, additional, Scanu, Antonio Mario, additional, Cossu-Rocca, Paolo, additional, De Miglio, Maria Rosaria, additional, and Uzzau, Sergio, additional

Published
2022
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14. Metaproteogenomic analyses of the gut microbiota in human and animal models: identification of changes induced by special diets in health and disease

Author

Abbondio, Marcello and Uzzau, Sergio

Subjects
MED/07 Microbiologia e microbiologia clinica
Abstract

Gut microbiota (GM) is known to have a role in immune system development, metabolism and protection from enteropathogen invasion. Characterizing the health-associated GM is key to formulate targeted interventions in case of dysbiotic states. As diet is one of the main factors with an impact on gut microbes, comprehending how it shapes the GM is also crucial. In view of this, through an integrated multi-omic approach based on 16S and shotgun metagenomics and metaproteomics, several studies were performed to obtain deeply characterized GMs in human, sheep and animal models and to shed light on specifically selected diet-GM interactions. First, the GM of a human healthy cohort and sheep were in-depth characterized, improving insights on taxonomy, active and taxon-specific functions. Further, butyrogenesis and glutamate degradation were detected as consistently active in all human subjects, becoming potential candidates for biomarkers of intestinal health. Second, the response of GM to caloric restriction (CR), and the GM possible changes at the onset of celiac disease (CD) were evaluated in animal models. Several changes solely due to CR were noted, regarding Lactobacillus spp. abundance and propionogenesis, while microbiota changes clearly related to the onset of CD were not observed. Lastly, preliminary data on two pilot studies on small human populations were reported, the first based on the analysis of different diet habits and the second as an actual dietary intervention.

Published
2018

16. Additional file 1: Table S1. of Potential and active functions in the gut microbiota of a healthy human cohort

Author

Tanca, Alessandro, Abbondio, Marcello, Palomba, Antonio, Fraumene, Cristina, Manghina, Valeria, Cucca, Francesco, Fiorillo, Edoardo, and Uzzau, Sergio

Abstract

Gender, age, and BMI of the human subjects selected for the study. Table S2. Metrics of metagenome and metaproteome analysis. Table S3. Taxonomic and functional annotation yields. Table S4. Percentage of taxa and functions with differential abundance between the human gut metagenomes and metaproteomes analyzed in this study. Table S5. Percentage distribution of conserved and variable features within the human gut metagenomes and metaproteomes analyzed in this study. (DOCX 35 kb)

Published
2017
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23. Atypical carcinoid and large cell neuroendocrine carcinoma of the lung: A proteomic dataset from formalin-fixed archival samples

Author

Tanca, Alessandro, Addis, Maria Filippa, Pisanu, Salvatore, Abbondio, Marcello, Pagnozzi, Daniela, Eccher, Albino, Rindi, Guido, Cossu Rocca, Paolo, Uzzau, Sergio, Fanciulli, Giuseppe, Rindi, Guido (ORCID:0000-0003-2996-4404), Tanca, Alessandro, Addis, Maria Filippa, Pisanu, Salvatore, Abbondio, Marcello, Pagnozzi, Daniela, Eccher, Albino, Rindi, Guido, Cossu Rocca, Paolo, Uzzau, Sergio, Fanciulli, Giuseppe, and Rindi, Guido (ORCID:0000-0003-2996-4404)

Abstract

Here we present a dataset generated using formalin-fixed paraffin-embedded archival samples from two rare lung neuroendocrine tumor subtypes (namely, two atypical carcinoids, ACs, and two large-cell neuroendocrine carcinomas, LCNECs). Samples were subjected to a shotgun proteomics pipeline, comprising full-length protein extraction, SDS removal through spin columns, in solution trypsin digestion, long gradient liquid chromatography peptide separation and LTQ-Orbitrap mass spectrometry analysis. A total of 1260 and 2436 proteins were identified in the AC and LCNEC samples, respectively, with FDR <1%. MS data are available in the PeptideAtlas repository at http://www.peptideatlas.org/PASS/PASS00375.

Published
2016

31. A first immunohistochemistry study of transketolase and transketolase-like 1 expression in canine hyperplastic and neoplastic mammary lesions.

Author

Burrai, Giovanni Pietro, Tanca, Alessandro, Cubeddu, Tiziana, Abbondio, Marcello, Polinas, Marta, Addis, Maria Filippa, and Antuofermo, Elisabetta

Subjects
IMMUNOHISTOCHEMISTRY, TRANSKETOLASE, PHOSPHOTRANSFERASES, ANTI-vaccination movement, PREVENTIVE medicine
Abstract

Background: Canine mammary tumors represent the most common neoplasm in female dogs, and the discovery of cancer biomarkers and their translation to clinical relevant assays is a key requirement in the war on cancer. Since the description of the 'Warburg effect', the reprogramming of metabolic pathways is considered a hallmark of pathological changes in cancer cells. In this study, we investigate the expression of two cancer-related metabolic enzymes, transketolase (TKT) and transketolase-like 1 (TKTL1), involved in the pentose phosphate pathway (PPP), an alternative metabolic pathway for glucose breakdown that could promote cancer by providing the precursors and energy required for rapidly growing cells. Results: TKT and TKTL1 protein expression was investigated by immunohistochemistry in canine normal (N = 6) and hyperplastic glands (N = 3), as well as in benign (N = 11) and malignant mammary tumors (N = 17). TKT expression was higher in hyperplastic lesions and in both benign and malignant tumors compared to the normal mammary gland, while TKTL1 levels were remarkably higher in hyperplastic lesions, simple adenomas and simple carcinomas than in the normal mammary glands (P < 0.05). Conclusions: This study reveals that the expression of a key PPP enzyme varies along the evolution of canine mammary neoplastic lesions, and supports a role of metabolic changes in the development of canine mammary tumors. [ABSTRACT FROM AUTHOR]

Published
2017
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33. Caloric restriction promotes rapid expansion and long-lasting increase of Lactobacillusin the rat fecal microbiota

Author

Fraumene, Cristina, Manghina, Valeria, Cadoni, Erika, Marongiu, Fabio, Abbondio, Marcello, Serra, Monica, Palomba, Antonio, Tanca, Alessandro, Laconi, Ezio, and Uzzau, Sergio

Abstract

ABSTRACTPrevious studies indicated that caloric restricted diet enables to lower significantly the risk of cardiovascular and metabolic diseases. In experimental animal models, life-long lasting caloric restriction (CR) was demonstrated to induce changes of the intestinal microbiota composition, regardless of fat content and/or exercise. To explore the potential impact of short and long-term CR treatment on the gut microbiota, we conducted an analysis of fecal microbiota composition in young and adult Fisher 344 rats treated with a low fat feed under ad libitum(AL) or CR conditions (70%). We report here significant changes of the rat fecal microbiota that arise rapidly in young growing animals after short-term administration of a CR diet. In particular, Lactobacillusincreased significantly after 8 weeks of CR treatment and its relative abundance was significantly higher in CR vs AL fed animals after 36 weeks of dietary intervention. Taken together, our data suggest that Lactobacillusintestinal colonization is hampered in AL fed young rats compared to CR fed ones, while health-promoting CR diet intervention enables the expansion of this genus rapidly and persistently up to adulthood.

Published
2018
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34. Metaproteogenomics Reveals Taxonomic and Functional Changes between Cecal and Fecal Microbiota in Mouse

Author

Tanca, Alessandro, Manghina, Valeria, Fraumene, Cristina, Palomba, Antonio, Abbondio, Marcello, Deligios, Massimo, Silverman, Michael, and Uzzau, Sergio

Subjects
gut microbiome, metabolic pathways, metagenomics, metaproteomics, microbial community, systems microbiology
Abstract

Previous studies on mouse models report that cecal and fecal microbial communities may differ in the taxonomic structure, but little is known about their respective functional activities. Here, we employed a metaproteogenomic approach, including 16S rRNA gene sequencing, shotgun metagenomics and shotgun metaproteomics, to analyze the microbiota of paired mouse cecal contents (CCs) and feces, with the aim of identifying changes in taxon-specific functions. As a result, Gram-positive anaerobes were observed as considerably higher in CCs, while several key enzymes, involved in oxalate degradation, glutamate/glutamine metabolism, and redox homeostasis, and most actively expressed by Bacteroidetes, were clearly more represented in feces. On the whole, taxon and function abundance appeared to vary consistently with environmental changes expected to occur throughout the transit from the cecum to outside the intestine, especially when considering metaproteomic data. The results of this study indicate that functional and metabolic differences exist between CC and stool samples, paving the way to further metaproteogenomic investigations aimed at elucidating the functional dynamics of the intestinal microbiota.

Published
2017
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