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A new C-glycosylflavone, identified as 6"'-O-acetylisospinosin (1), was isolated from the aerial parts of Cladanthus mixtus together with 30 known compounds. The structures of these compounds were established by interpretation of their spectral data, mainly UV, 1D and 2D NMR spectroscopic methods including (1H and 13C NMR, COSY, ROESY, HSQC and HMBC experiments), ESI-MS, and by comparison with the literature data.

International audience; A new phorbol-type diterpene ester, 4,20-dideoxy-4α-phorbol-12β-acetate-13α-isobutyrate, in addition to 11 known compounds were isolated from the latex and roots of Euphorbia clementei Boiss. Structure elucidation was performed by comprehensive 1D and 2D NMR analyses (1H and 13C NMR, COSY, ROESY, HSQC and HMBC experiments), mass spectrometry (HR-ESI-MS) and by comparison with literature data. The inhibitory activity of all isolated compounds was evaluated against promyelocytic leukemia HL60 and human erythromyeloblastoid leukemia K562 cell lines and seven of these compounds exhibited a weak cytotoxicity with IC50 values ranging from 40 to 97 μM.

A previously undescribed phenolic sulphate ester, potassium 34-dihydroxy-3-methoxybenzoic acid methyl ester-5-sulphate (1), along with nine known flavonoids, kaempferol-3-O-potassium sulphate-4′,7-dimethyl ether (2), kaempferol-4′,7-dimethyl ether (3), rhamnocitrin-3-O-potassium sulphate (4), rhamnocitrin (5), kaempferol (6), quercetin (7), afzelin (8), quercetin-3-O-α-l-rhamnopyranoside (9) and luteolin-3′-O-potassium sulphate (10) were isolated from the aerial parts of Tamarix balansae. Structures elucidation was performed by comprehensive 1D and 2D NMR analyses, mass spectrometry and by comparison with literature data. The antibacterial assay against Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa bacteria showed a good activity for 2, 3, 7 and 9, with MICs ranging from 62.5 to 250 μg/mL. The abilities of these compounds to scavenge the DPPH were evaluated. Compounds 1, 7, 9 and 10 exhibited a good antiradical activity potential with IC50 values ranging from 3.0 to 115.5 μg/mL, compared with ascorbic acid (IC50 7.4 μg/mL) which was used as positive control.

International audience; Two new phenolic glucosides, together with six known compounds, were isolated from the aerial part of Eryngium tricuspidatum L. (Apiaceae). The structures of the new compounds were established as 2-hydroxy- 3,5-dimethyl-acetophenon-4-O-β-d-glucopyranoside (1) and 2,3-dimethyl-4-hydroxymethylphenyl-1-hydroxymethyl-O-β-d-glucopyranoside (2) on the basis of detailed spectroscopic data including MS, 1D, and 2D NMR. The antibacterial, tyrosinase inhibitory and DPPH radical scavenging activities of hydromethanolic extract, fractions, and the eight isolated compounds were evaluated. The antibacterial assay showed a moderate activity for magnolioside (4) against Staphylococcus aureus CIP 53.154. Compound 7 (quercetin 3-O-β-d-glucopyranosyl-(1 → 6)-O-β-d-galactopyranoside) had moderate DPPH radical scavenging activity whereas compounds 2 exhibited good inhibitory effect against mushroom tyrosinase.

yellowish essential oil, having an intense and penetrating odor. Forty-one components were identified, representing 99.8% of the essential oil mainly characterized by camphor (27.4%), santolina triene (13.0%), thujone (12.9%), camphene (10.7%), and -curcumene (5.3%) (Table 1). Gas Chromatography-Mass Spectrometry. GC-MS was performed using a Shimadzu QP5050 mass selective detector and a cross-linked DB5-MS column (40 m 0.18 mm, film thickness 0.18 m). The oven temperature was programmed as isothermal at 60C for 5 min, then raised to 275C at 5C/min and held at this temperature for 5 min. Helium was used as the carrier gas at a rate of 1 ml/min. The oil (0.1 L) was introduced directly into the source of the MS via a transfer line (280C) with a split ratio of 1:50 and a linear velocity of 30.0 cm/sec. Ionization was obtained by electron impact (70 eV, source temperature 200C, resolution 1000). The present composition is quite different from the reported essential oil of Cotula coronopifolia L. leaves, which are mainly characterized by agarospirol (10.4%), and by hexacosane (31.7%) in flowers, and by 1-eicosanol (17.1%) in stems. The major constituents identified in the root oils were heptacosane (28.4%), 1-eicosanol (14.6%), octacosane (5.4%), and -amorphen (5.2%) [5].

Convolvulus is a fairly large genus with 250 species, almost cosmopolitan in distribution 1. Various biological activities such as anticancer [2], antinociceptive [3], antidiarrhea [4], and antifungal [5] have been reported for Convolvulus species, which are characterized by the presence of phenolic compounds and alkaloids [6–9]. Algerian flora is represented by 17 species [10]. We have been interested in the phytochemical study and antioxidant activity of the endemic species Convolvulus supinus Coss. & Kralik (Convolvulaceae). Aerial parts (1.5 kg) of Convolvulus supinus were macerated in a methanolic solution (80%) at room temperature. The extract was concentracted under low pressure, diluted with water, and filtered, then successively extracted with dichloromethane, ethyl acetate, and n-butanol. The butanolic extract (12 g) was column chromatographed on polyamid SC 6 , eluting with toluene–methanol with increasing polarity. A total of 103 fractions was obtained and combined into 19 main fractions (according to the number, color, and R f of the sports). Fraction F-9 was separated by column chromatography on silica gel and eluted with an isocratic system of EtOAc–MeOH–H 2 O (10:1:0.5) to afford compounds 1 and 2. Fraction F-14 was subjected to column chromatography on silica gel and eluted with an isocratic system of EtOAc–MeOH–H 2 O (10:1:1.5) to yield compound 3. These compounds were identified by the use of spectral methods: UV, 1 H NMR, and 13 C NMR in addition to acid hydrolysis.

A new phorbol-type diterpene ester, 4,20-dideoxy-4α-phorbol-12β-acetate-13α-isobutyrate, in addition to 11 known compounds were isolated from the latex and roots of Euphorbia clementei Boiss. Structure elucidation was performed by comprehensive 1D and 2D NMR analyses (1H and 13C NMR, COSY, ROESY, HSQC and HMBC experiments), mass spectrometry (HR-ESI-MS) and by comparison with literature data. The inhibitory activity of all isolated compounds was evaluated against promyelocytic leukemia HL60 and human erythromyeloblastoid leukemia K562 cell lines and seven of these compounds exhibited a weak cytotoxicity with IC50 values ranging from 40 to 97 μM.

A new phorbol-type diterpene ester, 4,20-dideoxy-4α-phorbol-12β-acetate-13α-isobutyrate, in addition to 11 known compounds were isolated from the latex and roots of Euphorbia clementei Boiss. Structure elucidation was performed by comprehensive 1D and 2D NMR analyses (1H and 13C NMR, COSY, ROESY, HSQC and HMBC experiments), mass spectrometry (HR-ESI-MS) and by comparison with literature data. The inhibitory activity of all isolated compounds was evaluated against promyelocytic leukemia HL60 and human erythromyeloblastoid leukemia K562 cell lines and seven of these compounds exhibited a weak cytotoxicity with IC50 values ranging from 40 to 97 μM.