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7 results on '"Aakansha Manav"'

1. Complete Genomic RNA Sequence of Tuberose Mild Mosaic Virus and Tuberose Mild Mottle Virus Acquired by High-Throughput Sequencing

Author

Malyaj R. Prajapati, Aakansha Manav, Pankhuri Singhal, Venkidusamy K. Sidharthan, Ujjwal Sirohi, Mukesh Kumar, Mahesh Kumar Bharti, Jitender Singh, Pankaj Kumar, Ravindra Kumar, Satya Prakash, and Virendra Kumar Baranwal

Subjects
tuberose, TuMMV, TuMMoV, high-throughput sequencing, phylogenetic analysis, Medicine
Abstract

Tuberose (Polianthes tuberosa) is an ornamental flowering crop of the Amaryllidaceae family. Tuberose mild mosaic virus (TuMMV) and tuberose mild mottle virus (TuMMoV), members of the genus Potyvirus, are ubiquitously distributed in most tuberose growing countries worldwide with low biological incidence. Here, we report the first coding-complete genomic RNA of TuMMV and TuMMoV obtained through high-throughput sequencing (HTS) and further, the presence of both the viruses were confirmed using virus-specific primers in RT-PCR assays. Excluding the poly (A) tail, the coding-complete genomic RNA of TuMMV and TuMMoV was 9485 and 9462 nucleotides (nts) in length, respectively, and contained a single large open reading frame (ORF). Polyprotein encoded by both the viral genomes contained nine putative cleavage sites. BLASTn analysis of TuMMV and TuMMoV genomes showed 72.40–76.80% and 67.95–77% nucleotide sequence similarities, respectively, with the existing potyviral sequences. Phylogenetic analysis based on genome sequences showed that TuMMV and TuMMoV clustered in a distinct clade to other potyviruses. Further studies are required to understand the mechanism of symptom development, distribution, genetic variability, and their possible threat to tuberose production in India.

Published
2022
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2. Identification and Characterization of a Garlic Virus E Genome in Garlic (Allium sativum L.) Using High-Throughput Sequencing from India

Author

Malyaj R. Prajapati, Aakansha Manav, Jitender Singh, Pankaj Kumar, Amit Kumar, Ravindra Kumar, Satya Prakash, and Virendra Kumar Baranwal

Subjects
garlic, high-throughput sequencing, RT-PCR, phylogenetic analysis, Botany, QK1-989
Abstract

Garlic (Allium sativum L.) plants exhibiting mosaics, deformation, and yellow stripes symptoms were identified in Meerut City, Uttar Pradesh, India. To investigate the viruses in the garlic samples, the method of high-throughput sequencing (HTS) was used. Complete genome of the garlic virus E (GarV-E) isolate (NCBI accession No. MW925710) was retrieved. The virus complete genome comprises 8450 nucleotides (nts), excluding the poly (A) tail at the 3′ terminus, with 5′ and 3′ untranslated regions (UTRs) of 99 and 384 nts, respectively, and ORFs encoding replicase with a conserved motif for RNA-dependent RNA polymerase (RdRP), TGB1, TGB2, TGB3, serine-rich protein, coat protein, and nucleic acid binding protein (NABP). The sequence homology shared 83.49–90.40% and 87.48–92.87% with those of GarV-E isolates available in NCBI at the nucleotide and amino acid levels, respectively. Phylogenetic analysis showed a close relationship of this isolate from India (MW925710) with GarV-E isolate YH (AJ292230) from Zhejiang, China. The presence of GarV-E was also confirmed by RT-PCR. The present study is the first report of GarV-E in garlic cultivar Yamuna Safed-3 grown in northern India. However, further studies are needed to confirm its role in symptom development, nationwide distribution, genetic diversity, and potential yield loss to the garlic in India.

Published
2022
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3. Identification of Garlic virus A infecting Allium sativum L. through next generation sequencing technology

Author

Amit Kumar, Pankaj Kumar, Malyaj R. Prajapati, Aakansha Manav, Koushlesh Ranjan, Jitender Singh, Ravindra Kumar, Virendra Kumar Baranwal, and Manoj Kumar Singh

Subjects
Crop, Veterinary medicine, food.ingredient, food, Genetics, food and beverages, Horticulture, Biology, Allium sativum, DNA sequencing, Virus, Allexivirus
Abstract

Garlic virus A (GarV-A) severely damages and reduces the yield of economically important garlic crop worldwide. Here, we present the first comprehensive view of GarV-A infecting Yamuna Safed-3 (G28...

Published
2021
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4. List of contributors

Author

Samuel Abebrese, Noemi Lizbeth Acuña-Flores, Mustafa Adhab, Joseph Adjebeng-Danquah, Parinita Agarwal, Pradeep K. Agarwal, Richard Yaw Agyare, Nawres A. Alkuwaiti, Benjamin Annor, Leonardo D. Arévalo-Monterrubio, José Trinidad Ascencio-Ibáñez, Alexandre Autechaud, Bhagirath M. Baraiya, Natalia Barboza, Mritunjoy Barman, Kwabena Asare Bediako, Eduardo R. Bejarano, Zineb Belabess, Sachin Ashok Bhor, Araceli G. Castillo, Supriya Chakraborty, Swati Chakraborty, Chinnaraja Chinnadurai, Aparna Chodon, Tathagata Choudhuri, Henryk Czosnek, Sarbani Das, Samantha de Jesus Rivero-Montejo, Ragunathan Devendran, Subham Dutta, Vincent N. Fondong, Elizabeth P.B. Fontes, Murad Ghanim, Prabu Gnanasekaran, Alireza Golnaraghi, Gokul Uttamgir Gosavi, Martine Granier, Ramón Gerardo Guevara-González, Vipin Hallan, Luko Hilje, Shridhar Hiremath, Yasir Iftikhar, Shaikhul Islam, Margaux Jammes, Jayaraj Jayaraman, Ajeet Kumar Jha, Jeyalakshmi Karanthamalai, Jawaid A. Khan, Zainul A. Khan, Mounika Kollam, Nagendran Krishnan, Aditya Kulshreshtha, Abhinav Kumar, Alok Kumar, Manish Kumar, R. Vinoth Kumar, Rakesh Kumar, Sailendra Kumar, Shweta Kumari, C.N. Lakshminarayana Reddy, Rosa Lozano-Durán, Israel Macias-Bobadilla, Lalit Mahatma, T. Makeshkumar, V.G. Malathi, Aakansha Manav, Anirban Mandal, Mahsa Mansourpour, M. Mantesh, Humberto Martínez-Montoya, Yamila Martínez-Zubiaur, Laura Mejía-Teniente, Leander Dede Melomey, Ritesh Mishra, M. Mohanraj, Prashant More, Mustansar Mubeen, Arindam Mukherjee, S. Nakkeeran, Michael Kwabena Osei, Koshlendra Kumar Pandey, Gopal Pandi, Harshalkumar P. Patel, Michel Peterschmitt, Malyaj R. Prajapati, Ved Prakash, null Priyanka, Nguyen Bao Quoc, Gabriel S. Raimundo, S.K. Raj, Adesh Ramsubhag, Koushlesh Ranjan, P. Renukadevi, Kumari Rhaeva, Luisa Katiana Rivas-Ramirez, Poonam Roshan, Nabanita Roy Chattopadhyay, Faustine Ryckebusch, Snigdha Samanta, B. Sangeetha, V.K. Satya, Nicolas Sauvion, Sangeeta Saxena, M. Senthil Alias Sankar, Niayesh Shahmohammadi, K.S. Shankarappa, Fredy Davi A. Silva, Jitender Singh, Sneha Sinha, Sunil Kumar Snehi, Ashish Srivastava, Sukumaran Sunitha, Jayanta Tarafdar, Jebasingh Tennyson, Ajay Kumar Tiwari, Reyna Ivonne Torres-Acosta, Rodolfo Torres-delosSantos, Irineo Torres-Pacheco, Eric Troadec, Muhammad Umer, Cica Urbino, Marcela Vargas-Hernandez, V. Venkataravanappa, Heshani De Silva Weligodage, Mengshi Wu, Sneha Yogindran, and Muhammad Ahmad Zeshan

Published
2022
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6. First report of natural infection by two potyviruses on amaryllis (Hippeastrum hybridum) plants from India

Author

Virendra Kumar Baranwal, Aakansha Manav, Malyaj R. Prajapati, Amit Kumar, Pankaj Kumar, Rajendra Pant, and Jitender Singh

Subjects
Leek yellow stripe virus, biology, Potyviridae, Potyvirus, food and beverages, New Records, biology.organism_classification, Amaryllis, Virus, Horticulture, Infectious Diseases, Hippeastrum, Genus, Virology, Allium
Abstract

Amaryllis plants showing the chlorotic stripes and yellowing of the leaves were collected. Electron microscopy of the infected plants revealed flexuous filamentous particles and disease incidence was recorded up to 64%. The presence of virus was confirmed using potyvirus genus and species-specific primers using Reverse transcription PCR. Blastn analysis showed that the samples were infected with leek yellow stripe virus (LYSV) and onion yellow dwarf virus (OYDV) the of genus Potyvirus, family Potyviridae. To the best of our knowledge, this is the first report of an association between LYSV (MZ203474 to MZ203478) and OYDV (MZ203479–MZ203482) with amaryllis. The result also showed that infected Amaryllis plants may act as a reservoir of LYSV and OYDV for economically important Allium crops.

Published
2021

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